CN111268241A - Cell freezing box - Google Patents

Cell freezing box Download PDF

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Publication number
CN111268241A
CN111268241A CN202010164533.9A CN202010164533A CN111268241A CN 111268241 A CN111268241 A CN 111268241A CN 202010164533 A CN202010164533 A CN 202010164533A CN 111268241 A CN111268241 A CN 111268241A
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CN
China
Prior art keywords
box
cell freezing
cover
cassette according
box body
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Granted
Application number
CN202010164533.9A
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Chinese (zh)
Other versions
CN111268241B (en
Inventor
张顺
蔡挺
庞建虎
史巧芸
施晓峰
廖振云
何丽荣
张沛瑶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningbo Huamei Hospital University of CAS
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Ningbo Huamei Hospital University of CAS
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Priority to CN202010164533.9A priority Critical patent/CN111268241B/en
Publication of CN111268241A publication Critical patent/CN111268241A/en
Application granted granted Critical
Publication of CN111268241B publication Critical patent/CN111268241B/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D11/00—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material
    • B65D11/02—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material of curved cross-section
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D11/00—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material
    • B65D11/18—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material collapsible, i.e. with walls hinged together or detachably connected
    • B65D11/1866—Containers having bodies formed by interconnecting or uniting two or more rigid, or substantially rigid, components made wholly or mainly of plastics material collapsible, i.e. with walls hinged together or detachably connected with detachable components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02—Internal fittings
    • B65D25/10—Devices to locate articles in containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D43/00—Lids or covers for rigid or semi-rigid containers
    • B65D43/02—Removable lids or covers
    • B65D43/08—Removable lids or covers having a peripheral flange fitting over the rim of the container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D43/00—Lids or covers for rigid or semi-rigid containers
    • B65D43/14—Non-removable lids or covers
    • B65D43/22—Devices for holding in closed position, e.g. clips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D53/00—Sealing or packing elements; Sealings formed by liquid or plastics material
    • B65D53/02—Collars or rings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Packages (AREA)

Abstract

The invention discloses a cell freezing box which comprises a box body, an accommodating cavity formed on the box body, a box cover detachably connected with the box body, a concave cavity formed on the box cover, a placing piece capable of being placed in the accommodating cavity, a plurality of first cavities formed on the placing piece, a cover body detachably connected with the box cover, and a plurality of second cavities formed on the cover body, wherein the first cavities correspond to the second cavities. According to the invention, the placing part and the cover body can be replaced, so that the placing part and the cover body with cavities of different sizes can be replaced when the freezing storage tubes of different sizes are masturbated and fixed, the replacement is convenient, and the cost is low.

Description

Cell freezing box
Technical Field
The invention belongs to the technical field of cell freezing, and particularly relates to a cell freezing box.
Background
The cell freezing is to ensure that the metabolism of the cells is basically suspended in a low-temperature environment for cell preservation, thereby ensuring that various biological characteristics of the cells are complete after long-term preservation for future use; at present, a plurality of cell freezing boxes exist in the market, a plurality of strip-shaped grooves are formed in the existing cell freezing boxes, when the cell freezing boxes are used, the freezing tubes are directly inserted into the grooves and then are covered with covers, but the existing freezing tubes have different sizes, the sizes of the grooves are certain, and therefore the freezing boxes with different sizes need to be replaced when the freezing tubes with different sizes are used.
Disclosure of Invention
The invention provides a cell freezing box which can adapt to freezing storage tubes with different sizes in order to overcome the defects of the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: a cell freezing box comprises a box body, an accommodating cavity formed in the box body, a box cover detachably connected with the box body, a cavity formed in the box cover, a placing piece capable of being placed in the accommodating cavity, a plurality of first cavities formed in the placing piece, a cover detachably connected with the box cover, and a plurality of second cavities formed in the cover, wherein the first cavities correspond to the second cavities.
According to the invention, the placing pieces are detachably connected with the box body, so that when the cryopreservation tubes with different sizes are stored, the first cavities with different numbers can be replaced according to the needs, and therefore, the same box body can be suitable for a plurality of placing pieces, and different cover bodies can be replaced according to the needs, so that the box body corresponds to the placing pieces; this mode is compared in traditional freezing box, only needs to purchase a small amount of box body and lid, purchases the piece and the lid of placing of sufficient quantity, and its is with low costs, and the availability factor is high to same box body can not change to the conduction of temperature, consequently when the cell is frozen, and when the box body used repeatedly, it is unanimous to carry out the effect of freezing to the cell, consequently can ensure the freezing effect when freezing to the cell.
Preferably, the bottom of the box body is provided with a first magnetic part, and the placing part is provided with a second magnetic part magnetically attracted with the first magnetic part; through setting up first magnetic part and second magnetic part, it is to placing a simple to operate, only need will place the piece put into the holding chamber in the middle of can to the magnetic part has the magnetic field, and it is to stimulatable cell, thereby ensures that the cell has certain activity before accomplishing the refrigeration, consequently the cell before freezing, homoenergetic enough is in and has under the active state.
Preferably, a first magnetic part is arranged on the box cover, and a second magnetic part magnetically attracted with the first magnetic part is arranged on the cover body; the cover body can be conveniently fixed through the first magnetic attraction piece and the second magnetic attraction piece, the cover body replacing efficiency is high, and the cover body is only required to be placed into the cavity.
Preferably, the upper end of the box body is provided with a first convex edge, and the lower part of the box cover is provided with a second convex edge, the side wall of which can be matched with the first convex edge.
Preferably, the second convex edge is provided with a sealing ring, and the first convex edge is provided with a sealing concave part for partially embedding the sealing ring; through setting up sealing ring and sealed concave part, it can ensure sealing performance, and then guarantee refrigeration effect.
Preferably, a through cavity is formed in the sealing ring, and sealing liquid which can be changed from a liquid state to a solid state at low temperature and is increased in volume is filled in the through cavity; can ensure through setting up sealed liquid when putting into the liquid nitrogen with box body and lid in the middle of, sealed liquid receives the cold solidification, and then the sealing ring inflation, closely laminates between sealing ring and the sealed concave part, consequently realizes sealing between box body and the lid, consequently ensures that the liquid nitrogen can progressively freeze the cell to the protection cell is not hurt.
Preferably, the sealing liquid is strong brine prepared in proportion; the sealing liquid is selected to be strong brine, which is easy to obtain and has low cost.
Preferably, a positioning convex part is arranged in the middle of the cover body, and a positioning cavity for the positioning convex part to insert is arranged on the placing piece; through setting up the location convex part and inserting can realize fixing a position lid and box body accuracy in the middle of the location chamber, consequently the lid with place and closely cooperate between the piece.
Preferably, a temperature gathering block is arranged in the positioning cavity, and a through groove part for liquid nitrogen to pass through and contact with the temperature gathering block is arranged at the bottom of the box body; through setting up logical slot part and gathering the temperature piece, the liquid nitrogen can follow logical slot part and gather the temperature piece and contact, consequently gather the cooling that the temperature piece can be rapid, and then when freezing the cell, can be from inside to outside transmission temperature, consequently freeze evenly to the cell.
Preferably, a plurality of anti-skid concave parts are arranged on the side wall of the box cover, and a plurality of anti-skid convex ribs are arranged on the anti-skid concave parts; through setting up anti-skidding concave part, will take off and the process of covering again for the lid, its and the laminating degree between the finger is high, and it is high to take the travelling comfort, sets up anti-skidding protruding muscle on anti-skidding concave part, has played anti-skidding effect.
In conclusion, the placing part and the cover body can be replaced, so that the placing part and the cover body with cavities of different sizes can be replaced when the freezing storage pipes of different sizes are pacified and fixed, the replacing is convenient, and the cost is low.
Drawings
Fig. 1 is a schematic structural diagram of a first embodiment of the present invention.
Fig. 2 is a cross-sectional view of a first embodiment of the present invention.
Fig. 3 is a state diagram of the case body and the case cover when they are opened according to the embodiment of the present invention.
Fig. 4 is an exploded view of the first embodiment of the present invention.
Fig. 5 is an exploded view of a partial structure according to an embodiment of the present invention.
FIG. 6 is a diagram of the second box and the lid of the second embodiment of the present invention when they are opened.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.
Example one
As shown in fig. 1-5, a cell freezing box comprises a box body 1, an accommodating cavity 10, a box cover 2, a placing member 3 and a cover body 4, wherein the box body 1 is made of polyurethane resin, and a cylindrical accommodating cavity 10 is formed on the box body 1; the bottom embedding of holding chamber 10 has first magnetic part 11, first magnetic part 11 is magnet, place the piece 3 and make for the polyurethane resin material equally place the bottom embedding on the piece 3 has second magnetic part 31, second magnetic part 31 with first magnetic part 11 can the magnetism attract mutually place have a plurality of first cavitys 30 on the piece 3, first cavity 30 is the cavity of cylindricality, prevents frostbite pipe insert in the middle of the first cavity 30.
Furthermore, the box cover 2 and the cover body 4 are both made of polyurethane resin, the box cover 2 is provided with a cavity 20, the bottom of the cavity 20 is provided with a first magnetic attraction piece 21, the cover body 4 is embedded with a second magnetic attraction piece 41, and when the cover body 4 is placed in the cavity 20, the first magnetic attraction piece 21 and the second magnetic attraction piece 41 attract each other, so that the box cover 2 and the cover body 4 are detachably connected; the cover 4 is provided with a plurality of second cavities 40, and the second cavities 40 correspond to the first cavities 30; the side wall of the box cover 2 has a plurality of anti-slip concave parts 24, and the side wall of the anti-slip concave parts 24 has a plurality of curved anti-slip ribs 241, and the anti-slip ribs 241 are integrally formed with the box cover 2.
Specifically, a positioning cavity 32 is formed in the middle of the placing member 3, a positioning protrusion 42 is arranged on the cover 4, and when the box cover 2 is connected with the box body 1, the positioning protrusion 42 is inserted into the positioning cavity 32; the upper end of the box body 1 is provided with a circle of first convex edge 12, the first convex edge 12 is a convex ring with the wall thickness thinner than that of the box body, and the first convex edge 12 and the box body 1 are integrally formed; a circle of sealing concave part 13 is arranged on the side wall of the first convex edge 12, and the sealing concave part 13 is a concave ring with an arc-shaped inner wall; the lower end of the box cover 2 is provided with a circle of second convex edge 22, the second convex edge 22 is a convex ring with the wall thickness smaller than that of the box cover, and the second convex edge 22 and the box cover 2 are integrally formed; a ring of sealing rings 23 is provided on the side wall of the second ledge 22.
The sealing ring 23 is a convex ring made of polyurethane resin, the sealing ring 23 is bonded to the second convex edge 22, the sealing ring 23 is provided with a through cavity 230, sealing liquid is filled in the through cavity 230, the sealing liquid is concentrated brine prepared according to a proportion, the sealing liquid can be changed from a liquid state to a solid state in liquid nitrogen, the volume of the sealing liquid can be increased, and therefore the sealing ring is abutted to the inner wall of the sealing concave portion, and sealing performance is achieved.
A temperature gathering block 321 is placed at the lower part of the positioning cavity 32, the temperature gathering block 321 is a metal round block, the bottom of the box body 1 is provided with a through groove part 14, and the through groove part 14 is a cylindrical cavity; part of the liquid nitrogen may enter from the through groove portion 14 and contact the temperature accumulating block 321, so that the temperature on the temperature accumulating block 321 may be transferred to the freezing tube.
Example two
As shown in fig. 6, the present embodiment is different from the first embodiment in that the number of the first cavities on the placing member is different, and the number of the second cavities on the cover body is different.
It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims (10)

1. The utility model provides a cell freezing box, includes box body (1), is formed in holding chamber (10) on box body (1), with box body (1) can dismantle lid (2) of connection and be formed in cavity (20) on lid (2), its characterized in that: the storage box is characterized by further comprising a placing piece (3) capable of being placed in the containing cavity (10), a plurality of first cavities (30) formed in the placing piece (3), a cover body (4) detachably connected with the box cover (2) and a plurality of second cavities (40) formed in the cover body (4), wherein the first cavities (30) correspond to the second cavities (40).
2. A cell freezing cassette according to claim 1, wherein: the box body (1) bottom is equipped with first magnetism spare (11), be equipped with on placing piece (3) with second magnetism spare (31) that first magnetism spare (11) magnetism attracted mutually.
3. A cell freezing cassette according to claim 2, wherein: the box cover (2) is provided with a first magnetic attraction piece (21), and the cover body (4) is provided with a second magnetic attraction piece (41) which is magnetically attracted with the first magnetic attraction piece (21).
4. A cell freezing cassette according to claim 1, wherein: the upper end of the box body (1) is provided with a first convex edge (12), and the lower part of the side wall of the box cover (2) is provided with a second convex edge (22) which can be matched with the first convex edge (12).
5. A cell freezing cassette according to claim 4, wherein: and a sealing ring (23) is arranged on the second convex edge (22), and a sealing concave part (13) for partially embedding the sealing ring (23) is arranged on the first convex edge (12).
6. A cell freezing cassette according to claim 5, wherein: the sealing ring (23) is provided with a through cavity (230), and sealing liquid which can be changed from liquid state to solid state at low temperature and is increased in volume is filled in the through cavity (230).
7. A cell freezing cassette according to claim 6, wherein: the sealing liquid is strong brine prepared according to a proportion.
8. A cell freezing cassette according to claim 1, wherein: the middle part of the cover body (4) is provided with a positioning convex part (42), and the placing piece (3) is provided with a positioning cavity (32) for inserting the positioning convex part (42).
9. A cell freezing cassette according to claim 8 wherein: a temperature gathering block (321) is arranged in the positioning cavity (32), and a through groove part (14) for liquid nitrogen to contact with the temperature gathering block is arranged at the bottom of the box body (1).
10. A cell freezing cassette according to claim 9, wherein: the anti-skidding box is characterized in that a plurality of anti-skidding concave parts (24) are arranged on the side wall of the box cover (2), and a plurality of anti-skidding convex ribs (241) are arranged on the anti-skidding concave parts (24).
CN202010164533.9A 2020-03-11 2020-03-11 Cell freezing box Active CN111268241B (en)

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Application Number Priority Date Filing Date Title
CN202010164533.9A CN111268241B (en) 2020-03-11 2020-03-11 Cell freezing box

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Application Number Priority Date Filing Date Title
CN202010164533.9A CN111268241B (en) 2020-03-11 2020-03-11 Cell freezing box

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CN111268241B CN111268241B (en) 2021-08-06

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112586493A (en) * 2020-11-18 2021-04-02 顺德职业技术学院 Box is collected to shower body of checking
CN113212936A (en) * 2021-04-27 2021-08-06 曹高博 Cell transportation is shifted and is deposited case with low temperature freezing

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110308271A1 (en) * 2010-06-18 2011-12-22 Biocision, Inc. Specimen freezing rate regulator device
WO2011159934A2 (en) * 2010-06-18 2011-12-22 Biocision, Inc. Specimen freezing rate regulator device
US20130025298A1 (en) * 2011-07-28 2013-01-31 Biocision, Inc. Preventing sample degradation from transient temperature fluctuations
CN103704199A (en) * 2013-09-30 2014-04-09 杭州柏恒科技有限公司 Cell freezing box
CN108990965A (en) * 2018-08-20 2018-12-14 四川妙和生物科技有限公司 A kind of cell cryopreservation box
CN212087796U (en) * 2020-03-11 2020-12-08 中国科学院大学宁波华美医院 Device for freezing cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110308271A1 (en) * 2010-06-18 2011-12-22 Biocision, Inc. Specimen freezing rate regulator device
WO2011159934A2 (en) * 2010-06-18 2011-12-22 Biocision, Inc. Specimen freezing rate regulator device
US20130025298A1 (en) * 2011-07-28 2013-01-31 Biocision, Inc. Preventing sample degradation from transient temperature fluctuations
CN103704199A (en) * 2013-09-30 2014-04-09 杭州柏恒科技有限公司 Cell freezing box
CN108990965A (en) * 2018-08-20 2018-12-14 四川妙和生物科技有限公司 A kind of cell cryopreservation box
CN212087796U (en) * 2020-03-11 2020-12-08 中国科学院大学宁波华美医院 Device for freezing cells

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112586493A (en) * 2020-11-18 2021-04-02 顺德职业技术学院 Box is collected to shower body of checking
CN113212936A (en) * 2021-04-27 2021-08-06 曹高博 Cell transportation is shifted and is deposited case with low temperature freezing

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