CN205223256U - Warm box of constant temperature - Google Patents
Warm box of constant temperature Download PDFInfo
- Publication number
- CN205223256U CN205223256U CN201520850092.2U CN201520850092U CN205223256U CN 205223256 U CN205223256 U CN 205223256U CN 201520850092 U CN201520850092 U CN 201520850092U CN 205223256 U CN205223256 U CN 205223256U
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- temperature
- heating
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- constant temperature
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Abstract
The utility model discloses an experimental device especially relates to a warm box of constant temperature, and including lid and box body, the lid upper shield is on the box body, the box body includes and shell and inner shell is equipped with the heat preservation between shell and inner shell, be equipped with the heat conduction partition plate that the level was placed inside the inner shell, heat conduction partition plate separates for sample layer and zone of heating inside the inner shell, the zone of heating includes heat accumulation medium, heating wire, insulating cover and control by temperature change probe, the last temp. Control module that installs of external surface of the housing, the control by temperature change probe is connected with temp. Control module, control by temperature change probe and inner shell be airtight connection, insulating ways is wrapped up in outside the heating wire, temp. Control module, heating wire and interior casing be airtight connection are connected to the heating wire, temp. Control module connects. This warm box of constant temperature can provide tentative constant temperature condition for the cell in experiment operation, be applicable to among the cell isolated culture experiment operation such as medical science, pharmacy, biology.
Description
Technical field
The utility model relates to a kind of experiment equipment, particularly relates to a kind of constant temperature and warms up box.
Background technology
Cell Culture Cells culture technique is also cell clone technology, and the regular noun in biology is cell culture technology.No matter for whole biotechnology, or the biological cloning technology of one of them, cell cultures is all a requisite process, the extensive clone of cell cultures inherently cell.Cell culture technology can be become the many cells of simply unicellular or few differentiation through mass propgation by a cell, this is the requisite link of clone technology, and the clone of cell cultures inherently cell.A large amount of cells or its meta-bolites is obtained by cell cultures.Because biological product is all get from cell, so can say that cell culture technology is most crucial in biotechnology, most basic technology.
Colony-formation assays condition comprises temperature, PH, osmotic pressure, nutrition, water, gas etc.When wherein temperature is too low, Growth of Cells does not slowly even grow, the growth conditions of low temperature effect cell, even also can affect the physiological change of cell.
In colony-formation assays experiment, need cell to be placed in 37 DEG C of incubators and cultivate.But recovering, go down to posterity, in the experimental implementation such as frozen, drug treating, need cell to take out from 37 DEG C of incubators, move into Bechtop and carry out experimental implementation, carry out in process in experiment, cell exposes at ambient temperature, and now temperature fluctuation becomes the factor affecting experimental result.
Also do not have a kind of volume little, convenient mobile in the market, the warm box of temporary transient constant temperature can be provided for cell in experimental implementation.
Utility model content
The utility model is intended to provide a kind of constant temperature to warm up box, can provide temporary transient constant temperature for cell in experimental implementation.
Base case in this programme is: constant temperature warms up box, comprises lid and box body, and lid upper shield is on box body; Box body comprises shell and inner casing, between shell and inner casing, be provided with thermal insulation layer; Inner housing inner is provided with the heat conduction baffle of horizontal positioned, and inner housing inner separates for sample layer and zone of heating by heat conduction baffle; Zone of heating comprises heat storage medium, nichrome wire, insulation covering and temperature probe; Outer shell outer wall is provided with temperature regulator, and the input end signal of temperature regulator is connected with the temperature probe gathering zone of heating temperature, and the output end signal of temperature regulator is connected with the rly. for controlling electric-heating-wire-heating; Temperature probe is connected with inner casing is airtight; Insulation covering cover is rolled in outside nichrome wire.Lid upper shield on box body, high insulating effect; Thermal insulation layer reduces heat loss, has both kept the relative constancy of temperature, again energy saving; Heat in zone of heating can be delivered to sample layer by heat conduction baffle; Nichrome wire and heat storage medium separate by insulation layer, shield; Nichrome wire energising heat release, heat storage is in heat storage medium; After the temperature that temperature probe is surveyed, by signal transmission to temperature control module, temperature control module controls the opening and closing of power supply.
Cover lid, to temperature control module design temperature 37 DEG C, to nichrome wire energising, heat storage medium temperature raises, and heat is delivered to sample layer by heat conduction baffle, and the temperature in sample layer raises; When the temperature that temperature probe is surveyed reaches 37 DEG C, by signal transmission to temperature control module, temperature control module auto-breaking, nichrome wire stops heating; When the temperature that temp probe is surveyed is lower than 37 DEG C, to temperature control module transmission of signal, temperature control module is energized automatically, electric-heating-wire-heating, and heat storage medium heats up.So, the temperature of zone of heating controlled at about 37 DEG C, sample layer temperature is slightly lower than 37 DEG C.In incubator, take out the culture dish of culturing cell, culture dish is placed on sample layer, cover lid, wait experimental implementation or not lid lid, directly carry out experimental implementation.
The beneficial effect of this programme is: this constant temperature warms up box can provide temporary transient constant temperature for cell in experimental implementation, and cell to warm up in box residing envrionment temperature at constant temperature and is more or less the same in incubator, reduces the impact of temperature on whole experimental error; This warm box body amasss little, convenient mobile, is convenient to use in experiment table or Bechtop.
Prioritization scheme one, based on the optimization of scheme, heat storage medium is sizing paraffin ball or surface-crosslinked type HDPE.These two kinds belong to matrix material heat storage medium, and matrix material heat storage medium does not need to encapsulate utensil, decreases packaging cost and encapsulation difficulty, avoid the danger that material leaks, add the security of materials'use, reduce the heat transfer resistance of container, be conducive to heat exchange.
Prioritization scheme two, as the optimization to prioritization scheme one, thermal insulation layer is built with the one in rock wool heat-preservation felt, polyurethane foam or phenol formaldehyde foam.Rock wool heat-preservation felt thermal conductivity is low; Polyurethane foam has insulation and water-proof function, and thermal conductivity is minimum in current all lagging materials, and its most outstanding feature is difficult combustion, low cigarette, high temperature resistance distortion; Phenol formaldehyde foam overcomes the shortcoming that original porous plastics type lagging material is inflammable, thickness, heat are out of shape, and remains the features such as original porous plastics type lagging material light weight, easy construction.Above three kinds of thermal insulation layers all can play good heat insulation effect.
Prioritization scheme three, as the optimization to prioritization scheme two, heat conduction baffle is the one in stainless steel separator, plastic septum or ceramic substrate.Stainless steel separator is heat-resisting, corrosion-resistant; Plastic septum moisture proof, corrosion-resistant, quality is light, loading strength is large; Ceramic substrate mechanical stress is strong, dimensionally stable, high thermal conductivity, high-insulativity, protection against corrosion.Above three kinds of heat conduction baffles all can meet not only heat conduction but also durable requirement.
Prioritization scheme four, as the optimization to prioritization scheme three, sample layer is provided with specimen holder.Culture dish layering can prevent by specimen holder, can improve the utilization ratio that constant temperature warms up box.
Accompanying drawing explanation
Fig. 1 is the sectional view that the utility model constant temperature warms up box embodiment one.
Embodiment
Below by embodiment, the utility model is described in further detail:
Reference numeral in Figure of description comprises: lid 10, shell 21, inner casing 22, thermal insulation layer 23, dividing plate 24, sample layer 25, zone of heating 26, specimen holder 251, sizing paraffin ball 261, insulation covering 262, temperature probe 31, temperature regulator 32.
Embodiment one is substantially as shown in Figure 1:
Constant temperature warms up box, comprises lid 10 and box body, and lid 10 upper shield is on box body; Box body comprises shell 21 and inner casing 22, is provided with thermal insulation layer 23 between shell 21 and inner casing 22, in thermal insulation layer 23, polyurethane foam is housed; Inner casing 22 inside is provided with the stainless steel separator 24 of horizontal positioned, and inner casing 22 inside separates for sample layer 25 and zone of heating 26 by stainless steel separator 24; Sample layer 25 is provided with specimen holder 251; Zone of heating 26 comprises sizing paraffin ball 261, nichrome wire, insulation covering 262 and temperature probe 31; Shell 21 outer wall is provided with temperature regulator 32, and the input end signal of temperature regulator 32 is connected with the temperature probe 31 gathering zone of heating 26 temperature, and the output end signal of temperature regulator 32 is connected with the rly. for controlling electric-heating-wire-heating; Temperature regulator 32 and rly. are arranged on the outside of shell 21, and it is inner that temperature probe 31 is arranged on zone of heating 26; Temperature regulator 32 also comprises the temperature indicator regulating temperature and displays temperature; Temperature probe 31 is connected with inner casing 22 is airtight; Insulation covering 262 cover is rolled in outside nichrome wire.Cover lid 10, to temperature regulator 32 design temperature 37 DEG C, be energized to nichrome wire, sizing paraffin ball 261 temperature raises, sizing paraffin ball 261 by phase transformation, by heat storage and slow releasing, the heat of release is delivered to sample layer 25 by heat conduction baffle 24, and the temperature in sample layer 25 raises; When the temperature that temperature probe 31 is surveyed reaches 37 DEG C, by signal transmission to temperature regulator 32, rly. auto-breaking, nichrome wire stops heating; Polyurethane foam trap heat can be delivered to external environment, makes the temperature variation of sample layer 25 and zone of heating 26 less; When the temperature that temp probe is surveyed obviously reduces, to temperature regulator 32 transmission of signal, rly. is energized automatically, electric-heating-wire-heating, and sizing paraffin ball 261 heats up.So, the temperature of zone of heating 26 controlled at about 37 DEG C, sample layer 25 temperature is slightly lower than 37 DEG C.In incubator, take out the culture dish of culturing cell, culture dish is placed on sample layer 25, cover lid, wait for experimental implementation.Cell to warm up in box residing envrionment temperature at constant temperature and is more or less the same in incubator, reduces the impact of temperature on whole experimental error.This constant temperature warms up box can provide the subenvironment of a temperature relative constancy for cell.
Embodiment two: the difference of the present embodiment and embodiment one is, heat storage medium adopts surface-crosslinked type HDPE; Rock wool heat-preservation felt is housed in thermal insulation layer; Heat conduction baffle adopts plastic septum.The present embodiment can realize the technique effect of constant insulation.
Embodiment three: the difference of the present embodiment and embodiment one is, in thermal insulation layer, phenol formaldehyde foam is housed; Heat conduction baffle adopts ceramic substrate.The present embodiment can realize the technique effect of constant insulation.
Above-described is only preferred implementation of the present utility model; should be understood that; for a person skilled in the art; under the prerequisite not departing from the utility model structure; some distortion and improvement can also be made; these also should be considered as protection domain of the present utility model, and these all can not affect effect and the practical applicability of the utility model enforcement.
Claims (5)
1. constant temperature warms up box, comprises lid and box body, and lid upper shield is on box body; It is characterized in that: box body comprises shell and inner casing, between shell and inner casing, be provided with thermal insulation layer; Inner housing inner is provided with the heat conduction baffle of horizontal positioned, and inner housing inner separates for sample layer and zone of heating by heat conduction baffle; Heat storage medium, nichrome wire, insulation covering and temperature probe is provided with in zone of heating; Outer shell outer wall is provided with temperature regulator, and the input end signal of temperature regulator is connected with the temperature probe gathering zone of heating temperature, and the output end signal of temperature regulator is connected with the rly. for controlling electric-heating-wire-heating; Temperature probe is connected with inner casing is airtight; Insulation covering cover is rolled in outside nichrome wire.
2. constant temperature according to claim 1 warms up box, it is characterized in that: described heat storage medium is sizing paraffin ball or surface-crosslinked type HDPE.
3. constant temperature according to claim 2 warms up box, it is characterized in that: described thermal insulation layer is built with the one in rock wool heat-preservation felt, polyurethane foam or phenol formaldehyde foam.
4. constant temperature according to claim 3 warms up box, it is characterized in that: described heat conduction baffle is the one in stainless steel separator, plastic septum or ceramic substrate.
5. constant temperature according to claim 4 warms up box, it is characterized in that: described sample layer is provided with specimen holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520850092.2U CN205223256U (en) | 2015-10-30 | 2015-10-30 | Warm box of constant temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520850092.2U CN205223256U (en) | 2015-10-30 | 2015-10-30 | Warm box of constant temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205223256U true CN205223256U (en) | 2016-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520850092.2U Active CN205223256U (en) | 2015-10-30 | 2015-10-30 | Warm box of constant temperature |
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| Country | Link |
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| CN (1) | CN205223256U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893666A (en) * | 2017-04-24 | 2017-06-27 | 何向锋 | A kind of multi-functional biomedical instrument |
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2015
- 2015-10-30 CN CN201520850092.2U patent/CN205223256U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893666A (en) * | 2017-04-24 | 2017-06-27 | 何向锋 | A kind of multi-functional biomedical instrument |
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