CN111685105A - Isolated organ keeps device - Google Patents
Isolated organ keeps device Download PDFInfo
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- CN111685105A CN111685105A CN202010703877.2A CN202010703877A CN111685105A CN 111685105 A CN111685105 A CN 111685105A CN 202010703877 A CN202010703877 A CN 202010703877A CN 111685105 A CN111685105 A CN 111685105A
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- isolated organ
- light source
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0294—Electromagnetic, i.e. using electromagnetic radiation or electromagnetic fields
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Abstract
The utility model provides an isolated organ keeps device, relates to medical equipment technical field, including the casing, the inside of casing has the chamber of holding, holds the intracavity and is provided with the storage ware that is used for holding isolated organ, is used for setting up cell active liquid in the storage ware so that isolated organ can set up in the storage ware and lie in under the liquid level of cell active liquid, is provided with the low-light source that can the exitance low light on the internal face of casing, and the low-light source is used for shining the isolated organ in the storage ware. The isolated organ preservation device not only can effectively delay the inactivation speed of cells after the isolated organ is separated from the human tissue environment, but also can improve the activity of biological cells of the isolated organ, thereby effectively reducing the influence of the transportation process of the isolated organ on the isolated organ and further effectively improving the survival rate of the isolated organ in the organ transplantation operation.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to an isolated organ preservation device.
Background
Organ transplantation (Organ transplant) is the process of surgically transferring an Organ, either wholly or partially, from one individual to another. The organ donor may be a donor for organ transplantation, and may be a human or a human who has just died. One recipient of the organ is the recipient of the organ transplant. The goal of organ transplantation technology is to replace a damaged or dysfunctional organ of a recipient with a good organ from a donor.
In 1970, after scientists discovered the category of histocompatibility (i.e., the same effect on foreign body tissues), organ transplantation surgery was increasing. By 1989, organ transplantation techniques became mature. Uk council teaches that 1000 heart transplantation operations were performed over a period of approximately 10 years with a survival rate of approximately 80% over 5 years. In 1989, the first concurrent heart, liver and kidney surgery in the United states was performed.
Nowadays, large hospitals in China are gradually capable of performing organ transplantation operations on large organs. However, due to the particularity of organ transplantation, there are cases that the donor and the recipient are not in the same operation area or even in the same city. In this case, medical care personnel are required to cut the donor organ from the body and then to properly preserve the donor organ to the maximum extent and to transfer the donor to the recipient as soon as possible on the basis of the preservation of the donor organ activity, so as to perform surgical transplantation as soon as possible.
The prior art has been primarily directed to the success of transplantation by maintaining a preferred ambient temperature (typically a low temperature environment below 0 ℃) and sealing the donor organ to be transplanted by immersion in a specially formulated liquid that helps maintain cellular viability, to isolate contamination as much as possible and slow the rate of inactivation of cells in the donor organ. However, the prior art of organ preservation mainly tries to slow down the rate of cell inactivation, and there is no method to actively improve or stimulate the activity of organs during the ex vivo preservation of organs.
Disclosure of Invention
The invention aims to provide an isolated organ preservation device which not only can effectively delay the inactivation speed of cells after an isolated organ is separated from a human tissue environment, but also can improve the activity of biological cells of the isolated organ, thereby effectively reducing the influence of the transfer process of the isolated organ on the isolated organ and further effectively improving the survival rate of the isolated organ in an organ transplantation operation.
The embodiment of the invention is realized by the following steps:
the embodiment of the invention provides an isolated organ preservation device which comprises a shell, wherein a containing cavity is formed in the shell, a storage device used for containing an isolated organ is arranged in the containing cavity, a cell active liquid is arranged in the storage device, so that the isolated organ can be arranged in the storage device and is positioned below the liquid level of the cell active liquid, a weak light source capable of emitting weak light is arranged on the inner wall surface of the shell, and the weak light source is used for irradiating the isolated organ in the storage device. The isolated organ preservation device not only can effectively delay the inactivation speed of cells after the isolated organ is separated from the human tissue environment, but also can improve the activity of biological cells of the isolated organ, thereby effectively reducing the influence of the transportation process of the isolated organ on the isolated organ and further effectively improving the survival rate of the isolated organ in the organ transplantation operation.
Optionally, in a preferred embodiment of the present invention, a positioning groove is provided in the storage, and the positioning groove is adapted to the size of the profile of the isolated organ, so that the isolated organ is positioned in the storage.
Optionally, in a preferred embodiment of the present invention, the medical device further includes a plurality of optical fibers, a weak laser light source capable of emitting weak laser light is further disposed on an inner wall surface of the housing, the plurality of optical fibers are respectively communicated with the weak laser light source, end portions of the plurality of optical fibers are used for respectively protruding through a large blood vessel of the isolated organ and an opening of the main lumen or emitting the weak laser light into the isolated organ in a manner that an epidermis of the isolated organ is pierced, wherein a length of a portion of the optical fibers protruding into the isolated organ is between 0.1cm and 20 cm.
Optionally, in a preferred embodiment of the present invention, the optical fiber is a full body optical fiber.
Optionally, in a preferred embodiment of the present invention, a refrigerator for storing a refrigerating member for refrigerating the inside of the housing is further disposed inside the housing.
Optionally, in a preferred embodiment of the present invention, the refrigeration member includes at least one of a semiconductor refrigeration sheet, an ice bag, and an ice cube, and when the refrigeration member includes the semiconductor refrigeration sheet, the isolated organ preservation apparatus further includes a power source electrically connected to the semiconductor refrigeration sheet for supplying power to the semiconductor refrigeration sheet.
Optionally, in a preferred embodiment of the present invention, the wavelength of the weak light source is in a range of 500nm to 10000nm, and the power density of the weak light source is in a range of 0.01mw/cm2～500mw/cm2。
Optionally, in a preferred embodiment of the present invention, the weak light source has a power density in a range of 0.01mw/cm2～100mw/cm2。
Optionally, in a preferred embodiment of the present invention, the wavelength of the weak light source is in a range of 600nm to 700nm or 780nm to 1100nm, and the power density of the weak light source is in a range of 0.01mw/cm2～30mw/cm2。
Optionally, in a preferred embodiment of the present invention, the weak light sources are point light sources, and the point light sources are uniformly distributed on an inner wall surface of the housing.
The embodiment of the invention has the beneficial effects that:
this isolated organ keeps device includes the casing, the inside of casing has the chamber of holding, it is provided with the storage device that is used for holding isolated organ to hold the intracavity, be used for setting up cell activity liquid in the storage device, thereby make isolated organ can hold in the storage device, and can soak under the liquid level of cell activity liquid, with the inactivation speed of isolated pollution as far as possible and delay isolated organ cell after breaking away from human tissue environment effectively, form the closed environment that is used for holding the storage device that holds isolated organ and cell activity liquid through the casing, thereby avoid the adverse effect of external environment to the biological cell activity of isolated organ. On this basis, through setting up the weak light source that can the exitance weak light on the internal face of casing, shine the isolated organ that sets up in the storage ware, can also improve the biological cell activity of isolated organ to can reduce the influence of isolated organ transportation process to the isolated organ effectively, and then can improve the survival rate of isolated organ in the organ transplantation operation effectively.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic structural diagram of an isolated organ preservation apparatus according to an embodiment of the present invention;
FIG. 2 is a second schematic structural view of an isolated organ preservation apparatus according to an embodiment of the present invention;
FIG. 3 is a third schematic view of the isolated organ preservation apparatus according to the present invention;
FIG. 4 is a fourth view of the isolated organ preservation apparatus according to the present invention;
fig. 5 is a fifth schematic structural view of an isolated organ preservation apparatus according to an embodiment of the present invention.
Icon: 100-isolated organ preservation apparatus; 10-a housing; 11-a containment chamber; 20-a weak light source; 30-a storage; 31-a positioning groove; 40-a refrigerator; 41-a refrigerating member; 50-an optical fiber; 60-weak laser light source; 200-isolated organ.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; either directly or indirectly through intervening media, or may be internal to both elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Examples
Referring to fig. 1 to 5, the isolated organ preservation apparatus 100 of the present embodiment includes a housing 10, a receiving cavity 11 is formed in the housing 10, a storage device 30 for receiving an isolated organ 200 is disposed in the receiving cavity 11, a cell activating liquid is disposed in the storage device 30, so that the isolated organ 200 can be disposed in the storage device 30 and located under the liquid level of the cell activating liquid, a weak light source 20 capable of emitting weak light is disposed on an inner wall surface of the housing 10, and the weak light source 20 is used for irradiating the isolated organ 200 in the storage device 30. The isolated organ preservation device 100 not only can effectively delay the inactivation speed of cells after the isolated organ 200 is separated from the human tissue environment, but also can improve the activity of biological cells of the isolated organ 200, thereby effectively reducing the influence of the transportation process of the isolated organ 200 on the isolated organ 200 and further effectively improving the survival rate of the isolated organ 200 in the organ transplantation operation.
First, Low-level light therapy (LLLT) or Photobiomodulation (PBM) involves the use of light to promote tissue repair, reduce inflammation, and produce analgesia, typically using Low-power light sources (lasers or LEDs). Due to the low power (the specific power level is usually determined by the target tissue and is usually lower than 500mW), no significant temperature increase is caused in the treated tissue, so that the total tissue structure of the target tissue does not change significantly. LLLT/PBM differs from other light-based treatment methods in that it does not ablate, nor is it based on heating. It also differs from photodynamic therapy (PDT), which is based on the action of light exciting exogenous chromophores to produce toxic Reactive Oxygen Species (ROS).
When weak light (such as red light) used in LLLT acts on cells of an organ, the activity of the cells can be improved to a certain extent, so that the activity of biological cells is enhanced, the activity of the biological cells of the isolated organ 200 can be improved in the process of in vitro transfer of the isolated organ 200, the influence of the transfer process of the isolated organ 200 on the isolated organ 200 can be effectively reduced, and the survival rate of the isolated organ 200 in an organ transplantation operation can be effectively improved.
Second, therapeutic light sources (lasers or LEDs) used in medicine can be generally divided into two categories: weak light and strong light. Because of the different biological action mechanisms of strong light and weak light, the purposes and methods of clinical application are different. In the medical field, the intensity of light is not measured by the physical parameters of light itself (such as power and energy), but is distinguished by the intensity of biological effect produced after the light acts on biological tissues. After the biological tissue is irradiated by light, if irreversible damage to the biological tissue is directly caused, the light at the irradiated surface is called strong light; if the irreversible damage is not directly caused, it is called weak light.
Third, the isolated organ preservation apparatus 100 is used in a closed state of the housing 10, and preferably, needs to be closed or sealed to preserve heat (or cool) and prevent contamination of the housing 10. As shown in fig. 1 to 5, the container 30 containing the isolated organ 200 and the cell viability fluid can be opened by being covered on one side or being rotated to be accessible, as required. Regarding the sealing manner of the housing 10, as shown in fig. 1 to 5, a sealing adhesive tape may be added to the edge of the panel where the housing 10 needs to be closed or opened by rotation. The closing manner of the housing 10 may be various, for example, a cover (not shown) on the top of the housing 10 may be directly opened in a manner of being able to cover and close, and the cover (not shown) on the top may be directly opened when the container 30 containing the isolated organ 200 and the cell active liquid is required to be taken and placed. Of course, in other embodiments, any one of the multiple side surfaces or the top surface enclosing the housing 10 may be configured as a hinged panel, that is, the panel can rotate relative to the connection point with the housing 10 through a hinge assembly to open or close, wherein the panel may be a single panel (similar to a single-door structure) or two separate panels (similar to a double-door structure) that are oppositely disposed. As shown in fig. 1 to 5, the panel is selected as a plate body of an integral structure in consideration of the case 10 required to ensure good sealing. The housing 10 may be specially designed according to the shape of the isolated organ 200, or may be directly selected from a bag-shaped container, a box-shaped vessel, a cup-shaped vessel, a bottle-shaped vessel, etc. having a suitable size in the existing medical equipment.
Fourthly, as shown in fig. 1 to 5, a storage 30 for accommodating the isolated organ 200 is disposed in the accommodating chamber 11 (or inside the housing 10), and a cell activating liquid is disposed in the storage 30, so that the isolated organ 200 can be accommodated in the storage 30 and can be soaked under the liquid level of the cell activating liquid, thereby isolating contamination as much as possible and effectively delaying the inactivation speed of cells after the isolated organ 200 is separated from the human tissue environment.
Regarding the structure of the container 30, the container 30 should have at least an internal hollow structure so as to be able to contain the isolated organ 200 and the cellular active liquid through the hollow structure, which means that the container 30 has at least a bottom panel and a side panel enclosing the bottom panel to form a semi-closed structure. Further, the top of the container 30 may have a top panel that is opened or closed with the side panel, so that the isolated organ 200 and the cell viability fluid can be disposed in the container 30, and the isolated organ 200 and the cell viability fluid disposed in the container 30 can be prevented from being contaminated by the external environment during the transportation of the isolated organ 200, i.e., the container 30 should preferably be closed or sealed during the use of the isolated organ preservation apparatus 100. As for the sealing manner of the depository 30, a sealing rubber strip may be added to the edge of the panel to be opened of the depository 30 similarly to the sealing manner of the housing 10. Preferably, the specific shape of the storage 30 is specially designed according to the shape of the isolated organ 200, and a cup-shaped vessel or a bottle-shaped vessel with a suitable size in the existing medical equipment can be directly selected. It is noted that the depositories 30 shown in fig. 1-5 are schematic structures and should not be used to limit the actual structure of the depositories 30, and that a person skilled in the art should be able to make appropriate designs and selections of the structure and dimensions of the depositories 30 according to the above-mentioned text or with reference to the structure of the casing 10.
Regarding the material of the storage 30, the storage 30 may be preferably made of a light-transmitting material, so that the isolated organ 200 can obtain a better irradiation effect no matter where the weak light source 20 is disposed in the housing 10. Further, a placing plate or a placing table may be further added inside the housing 10, and the storage 30 is placed on the placing plate or the placing table, so that a certain distance is provided between the storage 30 and the bottom of the housing 10, and the weak light source 20 may be disposed not only on the top surface or the side wall surface of the housing 10, but also on the bottom surface of the housing 10. When the storage 30 is placed on the placing plate or the placing table and the weak light source 20 is disposed on the bottom surface of the housing 10, the placing plate or the placing table should be selected to use a light-transmitting material in addition to the storage 30 so that the weak light emitted from the weak light source 20 can sequentially transmit through the placing plate or the placing table and the storage 30 to irradiate the side of the isolated organ 200 contacting with the bottom of the storage 30. The transparent material can be selected from glass, plastic, etc.
Meanwhile, considering that the storage 30 is contaminated by the living organisms after the storage 30 is used, that is, after a certain storage 30 has accommodated a certain isolated organ 200, it is required to perform strict and complicated processes for completely cleaning and sterilizing the storage 30 contaminated by the living organisms due to gaps or dead corners formed in the storage 30, and therefore, it is preferable that the storage 30 be used as a disposable medical product and discarded after one use, and the discarded storage 30 should be collected and recycled as medical waste to prevent environmental pollution.
Fifth, as shown in fig. 1 to 5, in order to keep the isolated organ 200 below the liquid level of the cell activation fluid, the amount of the cell activation fluid should be based on the fact that the top of the isolated organ 200 can be at least immersed below the liquid level of the cell activation fluid, so as to avoid that when the amount of the cell activation fluid is small, a part of the isolated organ 200 is not immersed in the cell activation fluid, so that the cell activation fluid does not play a role in delaying the cell inactivation speed of the part of the isolated organ 200, and the cell inactivation speed of the part of the isolated organ 200 is faster than the cell inactivation speed of the part of the isolated organ 200 immersed in the cell activation fluid, which may result in a decrease in survival rate of the isolated organ 200 in the organ transplantation operation.
As described above, the isolated organ preservation apparatus 100 includes the housing 10, the housing 10 has the accommodating cavity 11 inside, the accommodating cavity 11 is provided with the storage device 30 for accommodating the isolated organ 200, and the storage device 30 is used for arranging the cell active liquid, so that the isolated organ 200 can be accommodated in the storage device 30 and can be soaked under the liquid level of the cell active liquid, so as to isolate the contamination as much as possible and effectively delay the inactivation speed of the cells after the isolated organ 200 is separated from the human tissue environment, and the closed environment of the storage device 30 for accommodating the isolated organ 200 and the cell active liquid is formed through the housing 10, so as to avoid the adverse effect of the external environment on the biological cell activity of the isolated organ 200. On this basis, through set up weak light source 20 that can emit the low light on the internal wall face of casing 10, shine the isolated organ 200 that sets up in storage device 30, can also improve the biological cell activity of isolated organ 200 to can reduce the influence of isolated organ 200 transportation process to isolated organ 200 effectively, and then can improve the survival rate of isolated organ 200 in the organ transplantation operation effectively.
In order to avoid the injury of the isolated organ 200 caused by the collision of the isolated organ 200 with the inner wall surface of the storage device 30 during the isolated transportation, as shown in fig. 1 to 5, in the present embodiment, a positioning groove 31 for positioning the isolated organ 200 is provided in the storage device 30, and the positioning groove 31 is adapted to the size of the isolated organ 200, so that the isolated organ 200 can be positioned in the storage device 30. Illustratively, the isolated organs 200 represented by the heart, the liver, the kidney, and the like, the isolated organs 200 represented by the severed finger, the severed toe, the severed limb, and the like, and the isolated organs 200 represented by the intestine, the esophagus, the blood vessel, and the like, which have a cavity, have different or even greatly different profile sizes, and at this time, the shape and the size of the positioning groove 31 should be specially designed according to the profile size of the specific isolated organ 200, so that the positioning groove 31 can perform better positioning and limiting functions, and the positioning failure caused by the non-matching of the shape and the size of the positioning groove 31 and the profile size of the isolated organ 200 is avoided.
Since the weak light source 20 irradiates the surface of the isolated organ 200 irradiated by the isolated organ 200, as shown in fig. 3 to 5, in order to not only improve the activity of the biological cells outside the isolated organ 200, but also improve the activity of the biological cells inside the isolated organ 200, in the present embodiment, the isolated organ preservation apparatus 100 further includes a plurality of optical fibers 50, as shown in fig. 3 and 5, a weak laser light source 60 capable of emitting weak laser light is further disposed on the inner wall surface of the housing 10 (as shown in fig. 4, the weak laser light source 60 may also be disposed on the inner wall surface of the storage 30 at a position corresponding to the opening of the main lumen and the large blood vessel of the isolated organ 200, so that the storage 30 and the optical fibers 50 can be used as disposable medical supplies and disposed after being used at one time), the plurality of optical fibers 50 are respectively communicated with the weak laser light source 60, and the ends of the plurality of optical fibers 50 are used for respectively passing through the large blood vessel of the, The opening of the main cavity extends into or emits weak laser to the inside of the isolated organ 200 in a mode of penetrating the epidermis of the isolated organ 200, wherein the length of the part of the optical fiber 50 extending into the isolated organ 200 is between 0.1cm and 20 cm.
It should be noted that a weak Laser (Low Level Laser) is a Laser that is relatively strong, and is also called a Low intensity Laser, which refers to a Laser with Low power density or Low energy radiation, and refers to a Low power Laser that does not cause irreversible damage to a target tissue when directly irradiated with such a Laser. Is often used as a physiological stimulus source in biomedicine for scientific research and clinical treatment. The radiation power of weak lasers is usually in the range of a few milliwatts to several hundred milliwatts, and it is generally of more interest to irradiate the intensity, i.e. the optical radiation power per unit area (mw/cm)2) The irradiation intensity of continuous laser applied to medical treatment and cosmetology is generally not more than dozens of mw/cm2Mostly several mw/cm2. The low-intensity laser may include He-Nc laser (wavelength 632.8nm), GaAlAs laser (wavelength 820nm, 830nm), GaAs laser (wavelength 904nm), Nd: YAG laser (wavelength 1064nm), etc., different types of lasers are selected, and the corresponding lasers for generating the laser are different, and those skilled in the art can select the laser according to actual needs, and there is no specific limitation.
Exemplarily, as shown in fig. 5, taking a liver as an example, the liver includes 5 large blood vessels, 5 optical fibers 50 connected to the weak laser light source 60 are disposed in the housing 10 in a one-to-one correspondence with the 5 large blood vessels of the liver, and the end of the optical fiber 50 that is blunt and away from the weak laser light source 60 is inserted into the 5 large blood vessels of the liver through the 5 large blood vessel fractures of the liver, so that the weak laser light source 60 can irradiate the interior of the liver, thereby improving the cell activity inside the liver (i.e., the isolated organ 200).
Alternatively, as shown in fig. 3 and 4, taking the kidney as an example, the kidney includes 2 large blood vessels and 1 ureter (i.e., main lumen), 3 optical fibers 50 connected to the weak laser light source 60 are disposed inside the housing 10 in one-to-one correspondence with the 2 large blood vessels and 1 ureter of the kidney, and the blunt end of the optical fiber 50 away from the weak laser light source 60 is inserted into the 2 large blood vessels and 1 ureter of the kidney via the 2 large blood vessels and 1 ureter fracture of the kidney, so that the weak laser light source 60 can irradiate the inside of the kidney, thereby improving the cell activity inside the kidney (i.e., the isolated organ 200).
Alternatively, in the case of the severed finger, the optical fiber 50 may be selected to be an optical fiber 50 with a small diameter (e.g., 0.4mm), and the sharp end of the optical fiber 50 away from the weak laser source 60 is directly penetrated into the severed finger through the skin surface of the severed finger, so that the weak laser source 60 can irradiate the inside of the severed finger, thereby improving the cell activity inside the severed finger (i.e., the isolated organ 200).
It should be noted that, firstly, the optical fiber 50 may be a whole body optical fiber, also called a side optical fiber, an illumination optical fiber, and its obvious feature is that the whole root is luminous, therefore, when the whole body optical fiber is inserted into a large blood vessel, a main channel of the isolated organ 200 or pricked into the epidermis of the isolated organ 200, the part of the whole body optical fiber inserted into the isolated organ 200 may not only emit weak laser from the end part, but also emit weak laser from the side wall of the whole body optical fiber, so that the range of the isolated organ 200 irradiated by the weak laser is larger. Of course, in other embodiments, the optical fiber 50 may be a conventional optical fiber, and the weak laser light may be emitted only through the end of the optical fiber 50 that extends into the great vessels, main lumen, or into the epidermis of the isolated organ 200.
Secondly, the optical fibers 50 should be disposed in one-to-one correspondence with the great vessels and the main channels of the isolated organ 200, that is, the number of the optical fibers 50 is in one-to-one correspondence with the number of the great vessels and the main channels of the isolated organ 200, and meanwhile, the positions of the optical fibers 50 are also in one-to-one correspondence with the positions of the great vessels and the main channels of the isolated organ 200, so that when the isolated organ 200 stored by the isolated organ storage apparatus 100 is different, the optical fibers 50 can be inserted into the great vessels and the main channels of the isolated organ 200 in one-to-one correspondence, and joints and broken points of the optical fibers 50 in the light transmission process are reduced as much as possible, thereby improving the stability of connection and transmission.
Third, as shown in fig. 3 to 5, when the optical fiber 50 is additionally provided in the isolated organ preservation apparatus 100, in order to enable the optical fiber 50 to be inserted into the great vessel and the main channel of the isolated organ 200 in a one-to-one correspondence manner, firstly, when the isolated organ 200 is placed in the storage 30, a placement angle capable of sufficiently exposing the great vessel and the main channel of the isolated organ 200 should be selected as much as possible, and secondly, since the storage 30 should be sealed as much as possible to prevent the isolated organ 200 and the cell viability fluid disposed in the storage 30 from being contaminated, a through hole for passing the optical fiber 50 therethrough may be correspondingly formed at a position near the top surface or the side surface of the storage 30 (i.e., a position higher than the liquid level of the cell viability fluid), and preferably, a circular sealing ring may be further provided in the through hole to enable the optical fiber 50 to extend into the storage 30 through the through hole, and simultaneously, a gap between the outer surface of the optical fiber 50 and the inner wall surface of the, thereby improving the sealing of the storage 30 as much as possible.
It should be noted that the end of the optical fiber 50 extending into the isolated organ 200 through the opening of the main lumen of the great vessel of the isolated organ 200 should be blunt, and the end of the optical fiber 50 penetrating into the isolated organ 200 through the epidermis of the isolated organ 200 should be sharp, so that the optical fiber 50 can be inserted into the isolated organ 200 to reduce the damage of the surrounding tissues.
In addition, the placing manner of the isolated organ 200 shown in fig. 1 to 5 is only illustrative and is not used to limit the actual placing manner of the isolated organ 200, and those skilled in the art should be able to reasonably design and select the placing manner of the isolated organ 200 according to the above description or actual situation.
As shown in fig. 2 to 5, in the present embodiment, a refrigerator 40 for storing a cooling member 41 is further provided inside the housing 10, and the cooling member 41 is used for cooling the inside of the housing 10. Of course, in other embodiments, the cooling element 41 may be directly placed inside the housing 10, without adding a special cooler 40, which has the advantage of saving certain manufacturing cost and space inside the housing 10 compared to adding a special cooler 40, but also has the disadvantage of possibly affecting the storage 30 or the weak light source 20.
It should be noted that, during the use of the isolated organ preservation apparatus 100, unlike the aforementioned housing 10 and storage device 30, the refrigerator 40 may have an open structure with at least one side capable of being opened, so that the refrigerating member 41 placed in the refrigerator 40 can release as much generated cold air to the inside of the housing 10 as possible, thereby reducing the ambient temperature inside the housing 10 as much as possible, and achieving the purpose of effectively delaying the cell inactivation speed of the isolated organ 200 by reducing the ambient temperature of the isolated organ 200. The refrigerator 40 may be an additional independent container for storing the refrigeration element 41, such as a cup-shaped container or a bottle-shaped container, or may be a plate-shaped structure directly disposed on the inner wall surface of the casing 10, for example, a plate body obliquely disposed on the inner wall surface of the casing 10 and forming an included angle with the inner wall surface of the casing 10, one end of the plate body is fixedly connected with the inner wall surface of the casing 10, and the other end of the plate body extends in a direction away from the inner wall surface of the casing 10, so that a receiving groove with a V-shaped cross section is defined between the plate body and the inner wall surface of the casing 10, and the refrigeration element 41 can be received in. Of course, in other embodiments, the refrigerator 40 may also be a closed structure, and a certain number of gaps are formed on a side surface or a top surface of the refrigerator 40, so that the refrigerating element 41 can only be accommodated in the refrigerator 40 and cannot move to the outside of the refrigerator 40 to collide with the housing 10 or the storage 30, and at the same time, the cold air generated by the refrigerating element 41 placed in the refrigerator 40 can be released to the inside of the housing 10, thereby achieving the purpose of reducing the ambient temperature inside the housing 10. It is noted that the refrigerator 40 shown in fig. 2 to 5 is a schematic structure and should not be used to limit the actual structure of the refrigerator 40, and those skilled in the art should be able to reasonably design and select the structure and size of the refrigerator 40 according to the above text or with reference to the structure of the housing 10/depository 30.
As shown in fig. 2 to 5, in the present embodiment, the cooling member 41 includes at least one of a semiconductor cooling plate, an ice bag, and an ice cube, and when the cooling member 41 includes a semiconductor cooling plate, the isolated organ preservation apparatus 100 further includes a power supply electrically connected to the semiconductor cooling plate for supplying power to the semiconductor cooling plate. When the refrigerating member 41 is selected as the ice bag and/or the ice cubes, only the ice bag and/or the ice cubes need to be placed in the refrigerator 40.
It should be noted that the semiconductor refrigeration piece, also called thermoelectric refrigeration piece, is a heat pump. Its advantages are no slide part, limited space, high reliability and no pollution of refrigerant. By using the Peltier effect of the semiconductor materials, when direct current passes through a galvanic couple formed by connecting two different semiconductor materials in series, heat can be absorbed and released at two ends of the galvanic couple respectively, and the aim of refrigeration can be fulfilled. The refrigerating technology which generates negative thermal resistance is characterized by no moving parts and higher reliability. Since the semiconductor refrigeration sheet is used for refrigeration by direct current acting on the couple, when the semiconductor refrigeration sheet is used as the refrigeration member 41, the isolated organ preservation device 100 further needs to have a power supply communicated with the semiconductor refrigeration sheet to provide direct current for the semiconductor refrigeration sheet.
Optionally, the wavelength range of the weak light source 20 is 500nm to 10000nm, and the power density range of the weak light source 20 is 0.01mw/cm2～500mw/cm2. Preferably, the weak light source 20 has a power density in the range of 0.01mw/cm2～100mw/cm2. In this embodiment, the wavelength range of the weak light source 20 is 600 nm-700 nm or 780 nm-1100 nm, and the power density range of the weak light source 20 is 0.01mw/cm2～30mw/cm2. It is to be noted that the wavelength and power density of the weak light source 20 can be reasonably selected and designed by those skilled in the art according to practical situations, and are not particularly limited herein.
It should be noted that mitochondria are the most absorptive of red light, and after red light irradiates the isolated organ 200, the catalase activity of mitochondria can be increased, so as to increase the metabolism of cells, so that the glycogen content is increased, the protein synthesis is increased, and the adenosine triphosphate decomposition is increased, thereby promoting the synthesis of cells, the healing of wounds and fester, and the clinical application is very wide.
As shown in fig. 1 to 5, in the present embodiment, the weak light sources 20 are point light sources, and the point light sources are uniformly distributed on the inner wall surface of the housing 10, so that each part of the isolated organ 200 can be uniformly irradiated by the weak light sources 20, and the biological cell activity of each part of the isolated organ 200 can be uniformly improved. Of course, in other embodiments, the weak light source 20 may also be a linear light source, the linear light sources may be arranged in parallel on the inner wall surface of the housing 10, and two adjacent linear light sources may be arranged at equal intervals, so that the extending directions of the two adjacent linear light sources are parallel and the distances between the two adjacent linear light sources are equal. Generally, the number of the line light sources may be set to 2-6 to maximize the effect of increasing the activity of the biological cells of the isolated organ 200. When the number of the linear light sources is less, the range of the linear light sources irradiating the isolated organ 200 may be smaller, and the purpose of remarkably improving the biological cell activity of the isolated organ 200 cannot be achieved; when the number of the line light sources is large, the heat generated when the line light sources irradiate the isolated organ 200 is large, which may affect the activity of the biological cells of the isolated organ 200.
It should be noted that the weak light source 20 may be selected from different illumination modes, for example, a continuous illumination mode of the weak light source 20 and a pulse illumination mode of the weak light source 20, where the continuous illumination mode refers to a continuous and stable weak light output, the illumination mode is suitable for any environment of the weak light source 20, and the pulse illumination mode refers to an intermittent weak light illumination. Both of the above two irradiation methods can make the isolated organ 200 reach the purpose of being irradiated by the weak light source 20.
In this embodiment, the housing 10 is opaque to light so as to avoid interference of the external environment light on the photobiological effect of the isolated organ 200. Specifically, the housing 10 may be made of a light-proof material, and/or the inner wall of the housing 10 may be provided with a light-absorbing layer to prevent light from being transmitted through the housing 10.
It should be noted that the casing 10 is made of opaque material, and/or a light absorbing layer is disposed on an inner wall surface of the casing 10, which specifically includes the following three conditions: 1. the housing 10 is made of opaque material, for example, the housing 10 can be made of metal, alloy, ceramic, etc. 2. The inner wall surface of the case 10 is provided with a light absorbing layer, and the inner wall surface of the case 10 may be coated with a light absorbing material, for example. 3. The housing 10 is made of opaque material, and the light absorbing layer is disposed on the inner wall surface of the housing 10, that is, under the precondition of no contradiction, the third condition is to freely combine the features included in the first two conditions, thereby achieving the above purpose. Illustratively, the housing 10 is made of a metal material and the inner wall surface of the housing 10 is coated with a light absorbing material, or the housing 10 is made of an alloy material and the inner wall surface of the housing 10 is coated with a light absorbing material, or the housing 10 is made of a ceramic material and the inner wall surface of the housing 10 is coated with a light absorbing material.
Second, when the light absorbing layer is disposed on the inner wall surface of the housing 10, wherever the weak light source 20 is disposed, the weak light emitted from the weak light source 20 can be absorbed when the weak light irradiates the inner wall surface of the housing 10, so that the weak light emitted from the weak light source 20 can avoid the loss of the energy of the weak light caused by the weak light source 20 emitting outside the housing 10.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The isolated organ preservation device is characterized by comprising a shell, wherein a containing cavity is formed in the shell, a storage device used for containing an isolated organ is arranged in the containing cavity, cell active liquid is arranged in the storage device, so that the isolated organ can be arranged in the storage device and is positioned below the liquid level of the cell active liquid, a weak light source capable of emitting weak light is arranged on the inner wall surface of the shell, and the weak light source is used for irradiating the isolated organ in the storage device.
2. The isolated organ preservation apparatus according to claim 1, wherein a positioning slot is provided in the holder, the positioning slot being sized to match a profile of the isolated organ to allow the isolated organ to be positioned within the holder.
3. The isolated organ preservation apparatus according to claim 1, further comprising a plurality of optical fibers, wherein the inner wall surface of the housing is further provided with a weak laser light source capable of emitting weak laser light, the plurality of optical fibers are respectively communicated with the weak laser light source, end portions of the plurality of optical fibers are used for respectively extending into the isolated organ through a large blood vessel of the isolated organ and an opening of the main lumen or emitting the weak laser light into the isolated organ through a manner of skin penetration of the isolated organ, and a length of a portion of the optical fibers extending into the isolated organ is between 0.1cm and 20 cm.
4. The isolated organ preservation apparatus according to claim 3, wherein the optical fiber is a full body optical fiber.
5. The isolated organ preservation device according to claim 1, wherein a refrigerator for storing a refrigeration member for refrigerating the interior of the housing is further provided to the interior of the housing.
6. The isolated organ preservation apparatus according to claim 5, wherein the refrigeration member comprises at least one of a semiconductor refrigeration plate, an ice bag, and an ice cube, and wherein the isolated organ preservation apparatus further comprises a power source electrically connected to the semiconductor refrigeration plate for supplying power to the semiconductor refrigeration plate when the refrigeration member comprises the semiconductor refrigeration plate.
7. The isolated organ preservation apparatus according to claim 1, wherein the weak light source has a wavelength ranging from 500nm to 10000nm, and the weak light source has a power density ranging from 0.01mw/cm2～500mw/cm2。
8. The isolated organ preservation apparatus according to claim 7, wherein the weak light source has a power density in the range of 0.01mw/cm2～100mw/cm2。
9. The isolated organ preservation apparatus according to claim 8, wherein the weak light source has a wavelength ranging from 600nm to 700nm or 780nm to 1100nm, and the weak light source has a power density ranging from 0.01mw/cm2～30mw/cm2。
10. The isolated organ preservation apparatus according to claim 1, wherein the weak light source is a point light source that is uniformly distributed on an inner wall surface of the housing.
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| CN202010703877.2A CN111685105A (en) | 2020-07-21 | 2020-07-21 | Isolated organ keeps device |
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| CN202010703877.2A CN111685105A (en) | 2020-07-21 | 2020-07-21 | Isolated organ keeps device |
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