CN218736823U - Simple and convenient storage device of human hair follicle stem cell - Google Patents
Simple and convenient storage device of human hair follicle stem cell Download PDFInfo
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- CN218736823U CN218736823U CN202221318997.1U CN202221318997U CN218736823U CN 218736823 U CN218736823 U CN 218736823U CN 202221318997 U CN202221318997 U CN 202221318997U CN 218736823 U CN218736823 U CN 218736823U
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Abstract
The utility model discloses a simple and convenient storage device of human-derived hair follicle stem cell relates to stem cell and preserves technical field, and this simple and convenient storage device includes storage cylinder, heating device, circulating device, the storage cylinder is used for depositing stem cell culture solution, heating device is arranged in keeping warm to the stem cell culture solution in the storage cylinder, the opening of storage cylinder uses the cover sealed, circulating device is used for making stem cell culture solution at the inside and outside circulation of storage cylinder, circulating device's junction is equipped with the thermometer, the thermometer is used for measuring to leave the temperature of the stem cell culture solution of storage cylinder. The utility model is used for stem cell's short-term storage can provide suitable environment for stem cell storage, avoids stem cell inactivation after unfreezing.
Description
Technical Field
The utility model relates to a stem cell preserves technical field, specifically is a simple and convenient storage device of human-derived hair follicle stem cell.
Background
The hair follicle stem cell is an adult stem cell existing in the bulge part of the external root sheath of the hair follicle, has the biological characteristics of self-renewal capacity and long period, and has strong proliferation capacity and multidirectional differentiation potential like other adult stem cells.
The hair follicle stem cells can promote wound healing, are mostly applied to scar repair in the medical field, and are also related reports for treating alopecia caused by hair follicle atrophy. The hair follicle stem cells are usually frozen and stored at ultralow temperature by liquid nitrogen before being transplanted, and are heated and thawed during treatment, because the stem cell transplantation operation cannot be immediately performed after thawing, the stem cells are extremely easy to inactivate in the process of waiting for the operation to start, so that the transplantation success rate is reduced, the hair follicle stem cells need to be stored in a proper environment after thawing, the activity of the cells is maintained, and the cells can be transplanted at any time.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: provides a simple and convenient storage device for human-derived hair follicle stem cells, which aims to solve the storage problem after the hair follicle stem cells are unfrozen.
The utility model adopts the technical scheme as follows:
a convenient storage device for human hair follicle stem cells comprises: the stem cell culture solution circulating device comprises a storage cylinder, a heating device and a circulating device, wherein the storage cylinder is used for storing a stem cell culture solution, the heating device is used for preserving the heat of the stem cell culture solution in the storage cylinder, an opening of the storage cylinder is sealed by using a cylinder cover, the circulating device is used for enabling the stem cell culture solution to circulate inside and outside the storage cylinder, a thermometer is arranged at the joint of the storage cylinder and the circulating device, and the thermometer is used for measuring the temperature of the stem cell culture solution leaving the storage cylinder.
Preferably, the heating device is an infrared heater, a heating pipe of the heating device surrounds the storage cylinder, the heating pipe is sealed by a glass cover, the glass cover is internally vacuumized, and one side of the glass cover, which is close to the storage cylinder, is transparent.
Preferably, a reflective layer is coated on one side of the glass cover far away from the storage cylinder.
Preferably, a heat insulation pad is arranged below the storage cylinder, and the heat insulation pad is made of a refractory material.
Preferably, the heat insulation pad is fixedly connected with the base of the heating device, the upper surface of the heat insulation pad is provided with a limiting protrusion, and the limiting protrusion is used for keeping the axes of the storage cylinder and the heating pipe coincident.
Preferably, the thermometer is an electronic thermometer, a temperature controller is arranged on a cylinder cover of the storage cylinder, a display screen and a temperature adjusting knob are arranged on the temperature controller, the display screen is used for displaying a measured value of the thermometer, and the temperature adjusting knob is used for adjusting the power of the heating device.
Preferably, the circulating device comprises a circulating pump, a liquid pumping pipe and a liquid sending pipe, the liquid pumping pipe is used for communicating the bottom of the storage cylinder with an inlet of the circulating pump, the liquid sending pipe is used for communicating the upper part of the storage cylinder with an outlet of the circulating pump, and the liquid pumping pipe and the liquid sending pipe are detachably connected with the storage cylinder.
Preferably, a sampling port is arranged on the circulating pump.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
(1) The whole device is in a sealed state, so that microorganisms in the external environment can be prevented from invading and competing for nutrients in the culture solution, the stem cell culture solution in the storage cylinder can be uniformly heated by arranging the infrared heating device on the outer side of the storage cylinder, the heating pipe is not in direct contact with the storage cylinder and the stem cell culture solution, and the problem that stem cells are inactivated due to overhigh temperature near a heat source of contact type heating devices such as an electric bar and the like can be avoided;
(2) By arranging the circulating device, the stem cell culture solution is uniformly mixed in the circulating process, the condition that nutrient substances in the culture solution are not uniformly distributed during standing can be avoided, and stem cells are prevented from gradually precipitating to the bottom of the storage cylinder;
(3) The glass cover is arranged outside the heating pipe, and the vacuumizing treatment is carried out, so that the heat conduction between the heating pipe and the storage cylinder can be further avoided, the side wall of the storage cylinder is prevented from being heated and increasing the temperature and exceeding the appropriate temperature for storing stem cells, and the stem cells near the inner wall of the storage cylinder are prevented from being inactivated due to overhigh temperature;
(4) The below of storage cylinder is equipped with the heat insulating mattress, can prevent that the heat from running off from the bottom of storage cylinder, solves the problem that storage cylinder bottom temperature is low on the one side, and the upper surface of heat insulating mattress is equipped with spacing arch for the axis that makes storage cylinder, heating pipe keeps the coincidence, and the outer wall that can ensure the storage cylinder is even with the heating pipe distance, makes stem cell culture liquid thermally equivalent.
Drawings
Fig. 1 is a perspective view of embodiment 1.
Fig. 2 is an enlarged view of a point a in fig. 1.
Fig. 3 is a front view structural diagram of embodiment 1.
Fig. 4 is an enlarged view of fig. 2 at B.
Fig. 5 is a perspective view of embodiment 2.
Fig. 6 is a perspective view of embodiment 3.
The mark in the figure is: 1. a storage cylinder; 2. a heating device; 3. a circulation device; 4. a thermometer; 5. a temperature controller; 101. a cylinder cover; 102. a heat insulating pad; 201. heating a tube; 202. a glass cover; 301. a circulation pump; 302. a liquid pumping pipe; 303. a liquid delivery pipe; 501. a display screen; 502. a temperature adjusting knob; 1021. a limiting bulge; 2021. a light-reflecting layer; 3011. a sampling port.
Detailed Description
To make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the present invention is provided with reference to the accompanying drawings and embodiments, and it should be understood that the specific embodiments described herein are only used for explaining the present invention, and are not used for limiting the present invention.
Example 1
As shown in the drawings, the present embodiment provides a simple storage device for human hair follicle stem cells, which is mainly used for short-term storage of human hair follicle stem cells and also for storage of other stem cells that need to be stored in an environment of 36-40 ℃, and the simple storage device includes: the device comprises a storage cylinder 1, a heating device 2 and a circulating device 3, wherein the storage cylinder 1 is in a cylindrical shape with an open upper part and is used for storing stem cell culture solution, the heating device 2 is used for preserving the heat of the stem cell culture solution in the storage cylinder 1 to maintain the stem cell culture solution at 36-40 ℃, the opening of the storage cylinder 1 is sealed by a cylinder cover 101 so as to prevent the stem cell preservation solution from contacting with the air outside the storage cylinder 1, the circulating device 3 is used for circulating the stem cell culture solution inside and outside the storage cylinder 1, a thermometer 4 is arranged at the joint of the storage cylinder 1 and the circulating device 3, and the thermometer 4 is used for measuring the temperature of the stem cell culture solution leaving the storage cylinder 1.
The side surface of the storage cylinder 1 is transparent, the heating device 2 is an infrared heater, the heating pipe 201 of the heating device 2 surrounds the outside of the storage cylinder 1, the heating pipe 201 is sealed by a glass cover 202, and the inside of the glass cover 202 is vacuumized to eliminate heat conduction between the heating pipe 201 and the storage cylinder 1 as much as possible, and one side of the glass cover 202 close to the storage cylinder 1 is transparent to make infrared rays emitted by the heating pipe 201 penetrate through the glass cover 202 as much as possible.
In order to reduce the infrared radiation in the direction away from the storage cylinder 1 and reduce the energy loss of the heating tube 201, a reflective layer 2021 is coated on the side of the glass cover 202 away from the storage cylinder 1, specifically, the reflective layer 2021 is a silver coating.
The thermometer 4 is an electronic thermometer, the barrel cover 101 of the storage barrel 1 is provided with a temperature controller 5, the temperature controller 5 is provided with a display screen 501 and a temperature adjusting knob 502, the thermometer 4 is connected with the temperature controller 5 through an electric wire, the measured value of the thermometer 4 is displayed through the display screen 501, and the temperature adjusting knob 502 is used for adjusting the power of the heating device 2 so as to adjust the temperature of the stem cell culture solution.
Circulating device 3 includes circulating pump 301, liquid suction pipe 302, liquid delivery pipe 303, and liquid suction pipe 302 is with the bottom of storage cylinder 1 and the entry intercommunication of circulating pump 301, and liquid delivery pipe 303 is with the upper portion of storage cylinder 1 and the export intercommunication of circulating pump 301, and liquid suction pipe 302, liquid delivery pipe 303 realize dismantling with storage cylinder 1 through the hickey and be connected, are convenient for pull down circulating device 3 from storage cylinder 1.
The human hair follicle stem cells need to be stored in an environment close to the body temperature before transplantation, and the stem cell culture solution can be maintained at 36-40 ℃ by the heating device 2 and the circulating device 3 so as to ensure the survival of the stem cells as far as possible; when the temperature measured by the thermometer 4 is close to 40 ℃, the heating power of the heating device 2 can be finely adjusted through the temperature adjusting knob 502, so that the heating power is slightly reduced, and the overtemperature is avoided; when the temperature measured by the thermometer 4 is close to 36 ℃, the heating power of the heating device 2 can be slightly increased by the temperature adjusting knob 502, and the temperature is prevented from being too low.
Example 2
In order to prevent heat loss from the bottom of the storage cylinder 1 and avoid the temperature of the stem cell culture fluid at the bottom of the storage cylinder 1 from being too low, a heat insulation pad 102 is arranged below the storage cylinder 1, the heat insulation pad 102 is made of alumina bubble brick, and in other embodiments, refractory materials such as high alumina brick and silica brick can be used.
The heat insulation pad 102 is fixedly connected with the base of the heating device 2, the upper surface of the heat insulation pad 102 is provided with a limiting protrusion 1021, the limiting protrusion 1021 is used for keeping the axes of the storage cylinder 1 and the heating pipe 201 coincident, and the distance between the heating pipe 201 and the outer wall of the storage cylinder 1 is kept consistent, so that the storage cylinder 1 is uniformly heated.
Example 3
In addition to example 1, the circulation pump 301 is provided with a sampling port 3011 from which a stem cell culture solution can be sampled to analyze data such as the distribution density of stem cells, the content of nutrients, and the content of harmful substances in the culture solution.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not intended to limit the present invention, and all modifications, equivalents, improvements and the like that are made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A simple and convenient storage device of human hair follicle stem cell, which is characterized by comprising: storage cylinder (1), heating device (2), circulating device (3), storage cylinder (1) is used for depositing stem cell culture liquid, heating device (2) are arranged in keeping warm to the stem cell culture liquid in storage cylinder (1), the opening of storage cylinder (1) uses cover (101) sealed, circulating device (3) are used for making stem cell culture liquid at storage cylinder (1) internal-external circulation, the junction of storage cylinder (1), circulating device (3) is equipped with thermometer (4), thermometer (4) are used for measuring to leave the temperature of the stem cell culture liquid of storage cylinder (1).
2. The convenient storage device for the human-derived hair follicle stem cells as claimed in claim 1, wherein the side surface of the storage cylinder (1) is transparent, the heating device (2) is an infrared heater, a heating pipe (201) of the heating device (2) is surrounded on the outer side of the storage cylinder (1), the heating pipe (201) is sealed by a glass cover (202), a vacuum is pumped in the glass cover (202), and the side of the glass cover (202) close to the storage cylinder (1) is transparent.
3. The convenient storage device for the human hair follicle stem cells as claimed in claim 2, wherein the glass cover (202) is coated with a reflective layer (2021) on the side far away from the storage cylinder (1).
4. The convenient storage device for the human hair follicle stem cells as claimed in claim 2, wherein a heat insulation pad (102) is arranged below the storage cylinder (1), and the heat insulation pad (102) is made of a refractory material.
5. The convenient storage device for the human-derived hair follicle stem cells as claimed in claim 4, wherein the heat insulation pad (102) is fixedly connected with the base of the heating device (2), the upper surface of the heat insulation pad (102) is provided with a limiting protrusion (1021), and the limiting protrusion (1021) is used for keeping the axes of the storage cylinder (1) and the heating pipe (201) coincident.
6. The convenient storage device for the human-derived hair follicle stem cells as claimed in any one of claims 1 to 5, wherein the thermometer (4) is an electronic thermometer, a temperature controller (5) is arranged on a cover (101) of the storage cylinder (1), a display screen (501) and a temperature adjusting knob (502) are arranged on the temperature controller (5), the display screen (501) is used for displaying the measured value of the thermometer (4), and the temperature adjusting knob (502) is used for adjusting the power of the heating device (2).
7. The convenient storage device for the human hair follicle stem cells as claimed in any one of claims 1 to 5, wherein the circulation device (3) comprises a circulation pump (301), a liquid suction tube (302) and a liquid delivery tube (303), the liquid suction tube (302) connects the bottom of the storage cylinder (1) with the inlet of the circulation pump (301), the liquid delivery tube (303) connects the upper part of the storage cylinder (1) with the outlet of the circulation pump (301), and the liquid suction tube (302) and the liquid delivery tube (303) are detachably connected with the storage cylinder (1).
8. The device for conveniently storing human hair follicle stem cells as claimed in claim 7, wherein the circulating pump (301) is provided with a sampling port (3011).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221318997.1U CN218736823U (en) | 2022-05-30 | 2022-05-30 | Simple and convenient storage device of human hair follicle stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221318997.1U CN218736823U (en) | 2022-05-30 | 2022-05-30 | Simple and convenient storage device of human hair follicle stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218736823U true CN218736823U (en) | 2023-03-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221318997.1U Active CN218736823U (en) | 2022-05-30 | 2022-05-30 | Simple and convenient storage device of human hair follicle stem cell |
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| CN (1) | CN218736823U (en) |
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2022
- 2022-05-30 CN CN202221318997.1U patent/CN218736823U/en active Active
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