CN110195015A

CN110195015A - A kind of anhydrous dry type recovery instrument of universal cell cryopreservation product and working method

Info

Publication number: CN110195015A
Application number: CN201910571814.3A
Authority: CN
Inventors: 何向锋; 陆响明; 王晓军
Original assignee: Saimute Biological Engineering (shanghai) Co Ltd
Current assignee: Saimute Biological Engineering (shanghai) Co Ltd
Priority date: 2019-06-26
Filing date: 2019-06-26
Publication date: 2019-09-03

Abstract

The invention discloses a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product, including instrument box and instrument case lid, the instrument box to be flexibly connected with the instrument case lid；Far infrared transmitter is installed in the instrument box；Rewarming platform device, the rewarming platform device are mounted on inside the instrument box, and rewarming platform device is for placing cell cryopreservation bag or cell cryopreservation tube；Head motion is mixed, the mixing head motion is installed in the instrument box bottom, and is connected with the rewarming platform device；Cooling flow generating device, the cooling flow generating device are installed in rear portion in the instrument box；Non-contact temperature sensor, the non-contact temperature sensor are installed in the instrument box.The present invention is suitable for the anhydrous dry type recovery application of cell cryopreservation bag or cell cryopreservation tube.

Description

A kind of anhydrous dry type recovery instrument of universal cell cryopreservation product and working method

Technical field

The present invention relates to cell recovery fields, and in particular to a kind of anhydrous dry type recovery instrument of universal cell cryopreservation product and Working method.

Background technique

With the development of Life Science, cell therapy technology is maked rapid progress, main including stem cell therapy and thin with CAR-T Born of the same parents are the immune cell therapy of representative.Cell involved in the preparation process of cell product freeze and defrosting of recovering.Cell recovery When defrosting, optimum operation is that the cell cryopreservation bag or cell cryopreservation tube that are just taken out by liquid nitrogen container are rapidly heated to 37 DEG C, is allowed to Most easily damaged -5 DEG C~0 DEG C of cell is run through, recrystallizes to avoid ice crystal to cell damage, leads to the dead of cell It dies.

Cell cryopreservation container is common cell cryopreservation tube and two kinds of cell cryopreservation bag.Cell cryopreservation tube how small freeze capacity Cell cryopreservation demand multiselect cell cryopreservation bag in 5ml, more than 5ml.Cell cryopreservation bag is more suitable for stem cell and is immunized thin The commercial applications of born of the same parents, such as CAR-T cell therapy.Currently, water-bath recovery mode or dry can be used in the recovery of cell cryopreservation bag Formula recovery mode, the former water-bath recovery mode need whole manual operation, can not carry out normalizing operation, and it is thin to be easy pollution Born of the same parents gradually eliminate and use, and the current product of the latter's dry type recovery mode is limited, and recovery mode is single, and recovery efficiency is still not to the utmost Such as people's will, also it is significantly improved and growth requirement.

Currently, the use of cell cryopreservation tube and cell cryopreservation bag is relatively broad, demand one kind can meet cell jelly simultaneously Deposit the instrument of bag and the anhydrous dry type recovery of high-flux cell cryopreservation tube.

In any heating device, thermal energy of the heat source in the form of three kinds of convection current, radiation and conduction passes to heating object Body.It is thermally conductive to refer to when object each section is directly contacted without relative displacement or different objects by material molecule, atom and free electricity The warm-up movement of the microcosmic particles such as son and the heat transfer phenomenon carried out.Convection current is by fluid motion, heat by a transmitting The phenomenon that another place.Either thermally conductive or convection current, it is necessary to directly contact or rely on conventional substances by cold and hot object Heat is transmitted for medium.But the principle of heat radiation is then entirely different, it be externally emit by body surface it is visible and can not The ray seen transmits heat.The transmission speed of radiant heating is fast, and not by any medium, thus greatly reduces thermal energy biography Loss during passing, to improve heat utilization rate.

In far infrared heating technology based on prominent radiant heating.When far infrared is irradiated to the object being heated, one Ray is divided to be reflected back, a part is pierced over.When the far infrared wavelength of transmitting and the absorbing wavelength of heating object When consistent, the object being heated largely absorbs far infrared, so that " resonance " occurs for interior of articles molecule and atom --- it generates Strong vibration, rotation, and vibrate and rotation increases object temperature, to reach heating purpose.

Far infrared is a kind of light being invisible to the naked eye, it, which is different from general infrared ray (near infrared ray), only has human body Thermotherapy effect；It is converted into Far-infrared spoke ray via thermal energy of the specific infra-red material expeditiously by arround；And 3~10 μm The far infrared of wave-length coverage can be absorbed by hydrone and the intracorporal organic molecule of biology, therefore energy can be passed into In vivo, induce generate a succession of pair of human health beneficial to physiological action.Using this mechanism, far infrared can be answered widely With in health care, referred to as " far infrared therapy ".

The energy transmitting mode of carbon fiber electric heating tube is based on far infrared radiation, and wherein far infrared radiation efficiency reaches 70% or more.Carbon fiber electric heating tube can give off the infrared energy that can heat substance after energization.The infrared ray of radiation Wavelength is 2.3~14 μm, and the far infrared line spectrum of this section of wavelength is referred to as " light of life ", accounts for 80% or more of whole wavelength.It It can be absorbed by hydrone and generate resonance friction fuel factor, realize the effect for quickly improving heated material temperature.Especially Can effectively activating human body histocyte, promote blood circulation, accelerate newly to be metabolized, the enhancing health-care effects such as immunocompetence.

Currently, the anhydrous of cell cryopreservation bag or cell cryopreservation tube is not carried out using Far-infrared ray radiation heating principle still Dry type recovery application.To provide, one kind is energy-efficient, can meet cell cryopreservation bag simultaneously and high-flux cell cryopreservation tube is anhydrous The instrument of dry type recovery, the present invention is therefore.

The patent " a kind of cell cryopreservation bag dry type recovery device and working method " of first the applicant's application, application No. is In 201910119077.3, which still has in actual use continues Optimal improvements place, and such as: product lacks Versatility can only carry out heating recovery to cell cryopreservation bag, and cannot carry out heating recovery to cell cryopreservation tube；In cell cryopreservation Quick response cooling processing can not be carried out after the completion of bag rewarming, the cell recovery effect under high temperature recovery mode may be influenced；Add Have to sacrifice part heating surface (area) (HS because of the presence of temperature probe on hot pallet, influences pallet heating efficiency；Temperature probe Thermometric can only be carried out to a bit, and cell cryopreservation bag bulk temperature situation of change cannot be monitored, being easy to happen erroneous judgement influences cell Resuscitation effect；Heating pallet directly contacted with cell cryopreservation bag to be recovered, it is understood that there may be airspace and influence heat conduct.

The patent " a kind of universal cell cryopreservation product dry type resuscitation system and working method " of first the applicant's application, Application No. is in 201910457107.1, which also has continuation Optimal improvements place in actual use, such as: General rewarming workbench includes wraping plate, heating pallet, soft silica gel heat conductive pad, flexible separating film, flexible fastening net, temperature control device Equal components, structure is complicated, and technology realizes that difficulty is big；In the cell recovery device, heat source is in the form of two kinds of convection current and conduction Thermal energy passes to cell cryopreservation bag or cell cryopreservation tube, lacks radiation thermal conduction mode, cell cryopreservation bag or cell cryopreservation tube Internal and surface cannot heat simultaneously；Cell cryopreservation bag Cryopreservation Technology is required it is high, if cell cryopreservation bag freeze it is not flat enough It is whole, with heating pallet it is flat between there is airspace and will affect heat conduction efficiency.

Summary of the invention

It is an object of the invention to provide a kind of anhydrous dry type recovery instrument of universal cell cryopreservation product and working method, in solution State one or more in prior art problem.

A kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to the present invention, comprising:

Instrument box and instrument case lid, the instrument box are flexibly connected with the instrument case lid；

Rewarming platform device, the rewarming platform device are mounted on inside the instrument box, and rewarming platform device is thin for placing Born of the same parents freeze bag or cell cryopreservation tube；

Head motion is mixed, the mixing head motion is installed in the instrument box bottom, and fills with the rewarming platform It sets and is connected；

Far infrared transmitter is installed on inside the instrument box, for heating；

Cooling flow generating device, the cooling flow generating device is installed in inside the instrument box, for freezing；

Non-contact temperature sensor, the non-contact temperature sensor are installed in inside the instrument case lid.

The present invention is suitable for the anhydrous dry type recovery application of cell cryopreservation bag or cell cryopreservation tube.

The present invention: by Far-infrared ray radiation heating, contact conduction heating and head-on warm heat air brush heating three kinds heating Two or three of combined application in rewarming mode, rewarming are high-efficient；With can independently operated refrigeration module, have cold air Cooling method is brushed, fast-refrigerating function can be performed；The anhydrous dry type recovery instrument complete machine of universal cell cryopreservation product of the invention Temperature control is accurate, and sertoli cell freezes the high temperature recovery mode of bag or cell cryopreservation tube, and rewarming time is short, and cell recovery motility rate is high； Built-in rewarming platform device is set using in instrument box, can carry out sticking up the formula of returning back and forth mixing movement, elastic network(s) bottom and elastic network(s) Lid cooperates or rewarming saddle cooperates with fixed trampoline, can fix the position of cell cryopreservation bag or cell cryopreservation tube, Mixed effect is good；Using non-contact temperature sensor to the cell cryopreservation bag or cell cryopreservation tube progress temperature in rewarming platform device Degree monitoring avoids contact temperature probe sacrifice also contacts area and there are potential poor contacts will affect temperature control effect Etc. drawbacks, can be taken into account under the cooperation of AI intelligent numerical control software for the anhydrous dry of cell cryopreservation bag and high-flux cell cryopreservation tube Formula resuscitative efforts.

In some embodiments, the far infrared transmitter is equipped with far infrared radiated element, including far infrared Line heating plate and far-infrared heating tube, can be emitting far-infrared after energization, and the far infrared radiated element includes biology It is charcoal, carbon fiber product, silicon carbide, metal tube, resistance band, tourmaline, far-infrared ceramic, semiconductor, enamel, quartz ampoule, gold-plated Quartz ampoule, devitrified glass lamp, metal oxide, ruby heating tube, carbon fiber electric heating tube or far infrared oriented strong radiator.

In some embodiments, the far infrared transmitter is the first emitter of far infrared and far infrared Second emitter, first emitter of far infrared are set in the instrument box, and the far infrared second is sent out Injection device is set to the instrument case lid, first emitter of far infrared and the second emitter of far infrared difference Set on the two sides up and down of the rewarming platform device；It further include reflector and gas collection reflector, first emitter of far infrared Set on the lower section of the rewarming platform device, the reflector is set to the lower section of first emitter of far infrared, described remote The second emitter of infrared ray is installed on the top of the rewarming platform device, and the gas collection reflector is set to the far infrared the Above two emitters.

In some embodiments, the reflector and the gas collection reflector are integrally in a watt arc, the gas collection reflection Cover includes cover frame, internal layer reflection lobes, outer layer reflection lobes, the internal layer reflection lobes be it is multiple, it is equidistant evenly distributed, each other Spacing distance 5mm~5cm, the outer layer reflection lobes be it is multiple, it is equidistant evenly distributed, be spaced each other distance 5mm~5cm, institute It states internal layer reflection lobes and misses one another with the outer layer reflection lobes and be arranged, the reflector is located at the far infrared On the downside of one emitter.The far infrared that first emitter of far infrared emits downwards can be reflected through the reflector Upwards, the gas collection reflector is located on the upside of second emitter of far infrared, the transmitting of far infrared second dress Lower section can be reflected towards through the gas collection reflector by setting the far infrared emitted upwards, and second emitter of far infrared adds The air of heat can circulate through the impeller clearance of the gas collection reflector, and hot-air is sent to the head-on rewarming fan by hose The back side, then by it is described head-on rewarming fan blowout.

In some embodiments, the rewarming platform device is rewarming rack, and the rewarming rack includes rack frame, elasticity Net bottom, elastic square are flexibly connected the elastic square on the rack frame, and the rack frame is flexibly connected the mixings shake Mechanism, the mixing head motion are fixedly mounted on bottom in the instrument box, and the rack frame is movably arranged on described mixed The tache motorice of even head motion, the rack frame middle part are equipped with the elastic network(s) bottom, the surrounding edge at the elastic network(s) bottom and institute It states rack frame inner edge to be connected, the elasticated net bottom is cross-woven by several warps and Ruo Kai weft, the elasticity square Including frame and elasticated net, the frame is flexibly connected with the rack frame, and the elasticated net is equipped in the middle part of the frame, described Elasticated net is cross-woven by several warps and Ruo Kai weft.The frame material of the rack frame and elastic square is good thermally conductive Metal material, preferably aluminium, light weight, intensity height, good heat conductivity.Far-infrared ray radiation heating and head-on warm heat air is blown It strokes two kinds of heating methods of heating to match, the rewarming effect of cell cryopreservation bag or cell cryopreservation tube is more preferable.

Both elastic network(s) bottom and elastic square are used cooperatively: first is that making cell cryopreservation bag or cell cryopreservation tube in rewarming rack Stick up when the formula of returning back and forth mixes movement will not slide；Second is that in the rewarming heating period, enable head-on blow-through warm heat air-flow it is straight It connects and brushes cell cryopreservation bag or cell cryopreservation tube, be conducive to cell cryopreservation bag or cell cryopreservation tube absorbs heat rapid rewarming, or Person enables cold airflow directly to brush cell cryopreservation bag or cell cryopreservation tube in the cooling down stage, is conducive to cell cryopreservation bag Or cell cryopreservation tube fast cooling；Three are easy for non-contact temperature sensor engraving by elastic network(s) bottom and/or elastic square Vacancy carries out wide-angle omni-directional temperature monitoring to rewarming rack overall work face, can take into account under the cooperation of AI intelligent numerical control software For the dry type resuscitative efforts of cell cryopreservation bag and high-flux cell cryopreservation tube, temperature control is more accurate.

In some embodiments, the far infrared transmitter quantity is one, including far infrared transmitter It is mounted in instrument case lid with gas collection reflector, the gas collection reflector is mounted on the upside of the far infrared transmitter；Institute Stating rewarming platform device is that double function heat rewarming apparatus, double function heating rewarming apparatus include rewarming saddle, double function heating pallet, Isolation film fixes trampoline and mixes head motion, and fixed trampoline, the rewarming saddle activity are flexibly connected on the rewarming saddle Connection mixes head motion, and the mixing head motion is fixedly mounted on bottom in the instrument box, and the rewarming saddle is living Dynamic to be mounted on the tache motorice for mixing head motion, the rewarming saddle surrounding edge, which extends, is equipped with the isolation film, described The upside of rewarming saddle is pallet side, and pallet side is equipped with double function and heats pallet, double function heating pallets and the rewarming Saddle is flexibly connected, and double function heating pallets are detachable and interchangeable, and double function heating pallets are with the rewarming saddle by connecing Head device electrical connection, double function heating pallets include tray frame and calandria, and the tray frame is made of metal, described Calandria is set in the tray frame, and the calandria is that there are conduction heating and two kinds of Far-infrared ray radiation heating to add simultaneously The electric hot plate of heat function, the electric hot plate include carbon-crystal electric hot plate, carbon fiber electrothermal plate etc., the calandria and the tray frame Frame is fixedly connected, and the tray frame is equipped with temperature control temperature sensor, and the temperature control temperature number of sensors is~it is a, it is described double The operating temperature range that function heats pallet is DEG C~DEG C, is painted with cell cryopreservation bag or cell cryopreservation tube on double function heating pallets Placement position guide line；It is connected between the surrounding edge of the isolation film and the instrument box inner wall, the rewarming support Platform and the isolation film are by two spaced-aparts up and down of the instrument box inner space.Present embodiment adds contact conduction Heat, Far-infrared ray radiation heating and blow-through warm heat air are brushed three kinds of rewarming modes of heating and are combined, cell cryopreservation bag or The rewarming of cell cryopreservation tube is more efficient；Double function heating rewarming apparatus overall weights are light, and structure is simpler, are easy to technology realization, Importantly, built-in double function heating rewarming apparatus, which facilitates, is equipped with air cooling function, cell cryopreservation bag or cell are frozen When depositing pipe selection high temperature rapid rewarming mode, it can be transferred to rapidly air cooling mode after rewarming, be conducive to cell viability Holding, the preferred aluminum of material of the frame of the rewarming saddles of double function heating rewarming apparatus, tray frame and fixed trampoline, weight Gently.

In some embodiments, the mixing head motion includes power source, connecting rod and bracket, and the power source is solid Surely it is connected to bottom in the instrument box, the power source includes servo motor and eccentric wheel, the both ends of the servo motor Equipped with the eccentric wheel, the number of the servo motor is 1~2, and the number of the connecting rod is 1~4, the eccentric wheel Number be 1~4, the eccentric wheel and the rewarming platform device are flexibly connected by the connecting rod by~a tie point, By the regulation of the numerical control device, the mixing head motion can drive the rewarming platform device real for the operation of the servo motor It now sticks up the formula of returning and back and forth mixes movement.Bracket is flexibly connected the rewarming platform device by 2 supporting points, constitutes two o'clock support type Rewarming platform device, bracket are flexibly connected the rewarming platform device by 4 supporting points, constitute the rewarming platform dress of four-point supporting type It sets, by changing the revolving speed of servo motor and the starting angle of operation of eccentric wheel, the rewarming platform device of two o'clock support type or at 4 points The rewarming platform device of support type may be implemented the complicated and diversified formula of returning of sticking up and back and forth mix movement.

In some embodiments, the cooling flow generating device includes cold airflow spout, cold water conservancy diversion rudder piece, cold air Road, cooling fan and refrigeration source, the cold water conservancy diversion rudder piece are mounted on the cold airflow spout, and the cold airflow spout is mounted on described The air outlet in cold air road, the cold air road are connected to the refrigeration source, and the refrigeration source connects with the cooling fan, the refrigeration source It can be refrigeration machine or low temperature bin.The refrigeration machine can be built-in refrigeration machine or circumscribed refrigeration machine, the built-in refrigeration machine Inside the universal anhydrous dry type recovery instrument of cell cryopreservation product, the circumscribed refrigeration machine is located at described universal thin Born of the same parents freeze outside the anhydrous dry type recovery instrument of product, and the refrigeration machine can be compression refrigerating machine, Absorption Refrigerator, steam injection Formula refrigeration machine or semiconductor refrigerator；Cryogenic substance can be placed in the low temperature bin, the low temperature bin can be built-in low temperature bin Or circumscribed low temperature bin, the built-in low temperature bin are set to inside the universal anhydrous dry type recovery instrument of cell cryopreservation product, The circumscribed low temperature bin is set to outside the universal anhydrous dry type recovery instrument of cell cryopreservation product, the cold airflow spout side To for the rewarming platform device；Compression refrigerating machine can be piston type, rotary, screw or centrifugal, and cryogenic substance can be Dry ice, liquid nitrogen, pre- freezing ice bag or ice cube, cooling flow generating device include regulating and controlling temperature and regulation wind speed function, refrigeration temperature control Effect is more preferable, and cooling flow generating device can make instrument box internal temperature reduce rapidly, and it is living to be conducive to raising cell recovery Rate.

In some embodiments, the non-contact temperature sensor is fixedly mounted in the instrument box, described The temperature monitoring direction of non-contact temperature sensor is the upside or downside of the rewarming platform device, the non-contact temperature There are Real Data Exchangs with the numerical control device for sensor.Among the above, non-contact temperature sensor includes thermopile array Non-contact temperature sensor, infrared temperature probe temperature sensor, thermal infrared imager temperature sensor etc.；Non-contact temperature Sensor be set to instrument container it is intracorporal above or below, can engraving by elastic network(s) bottom and/or elastic square and/or fixed trampoline Vacancy carries out wide-angle omni-directional temperature monitoring to overall work face above or below rewarming platform device, passes through non-contact detection rewarming The infrared heat feature of the cell cryopreservation bag or cell cryopreservation tube placed in platform device, and be converted and generate thermal image and temperature Value, and then show over the display, and temperature value can be calculated, timely feedback adjustment far infrared transmission device and rewarming The working condition of platform device.The rewarming progress feelings of cell cryopreservation tube or cell cryopreservation bag are monitored using non-contact temperature sensor Condition can both avoid contact with formula temperature probe sacrifice also contacts area and there are potential poor contacts to influence temperature control effect etc. Drawback, and the anhydrous dry type resuscitative efforts of high-flux cell cryopreservation tube can be competent under the cooperation of AI intelligent numerical control software.

In some embodiments, further include head-on rewarming fan, agitation fan and air temperature sensor；It is described to head on Rewarming fan, agitation fan and air temperature sensor are mounted in instrument box, and the head-on rewarming fan is for will be through Warm heat air after the heating of far infrared injection device head-on directly brushes the cell cryopreservation bag or cell cryopreservation of rewarming platform device Pipe surface, the agitation fan are used to air themperature parameter everywhere in instrument box tending to identical, and the air themperature passes Sensor is used for the intracorporal air themperature of at-once monitor instrument container, adjusts the intracorporal temperature of the instrument container.

In some embodiments, the side of the far infrared transmitter, institute is arranged in the head-on rewarming fan The blowing direction for stating head-on rewarming fan is head-on to blow to the rewarming platform device, and the number of the head-on rewarming fan is 1~4 It is a；The agitation fan is set to the intracorporal any position of the instrument container, and the number of the agitation fan is 1~4；The row Fan is set on the tank wall of the instrument box, and the number of the exhaust fan is 1~4；The head-on rewarming fan, stirring The start and stop of fan and exhaust fan receive the control of the numerical control device；The air temperature sensor is set to the instrument The intracorporal different location of device case, the number of the air temperature sensor are 1~4, the air temperature sensor with it is described There are Real Data Exchangs for numerical control device.Agitation fan is set to the intracorporal any position of the instrument container, generates rough air, makes Air themperature parameter tends to identical everywhere in instrument box；When the air temperature sensor monitors specified for temperature ranges When, the numerical control device can make corresponding control feedback according to real time temperature.

It in some embodiments, further include exhaust fan, numerical control device, DEU data exchange unit and touch screen, the air draft Fan is mounted on the instrument container body wall, and the numerical control device and the DEU data exchange unit are mounted in the instrument box, The touch screen is installed on the instrument box；

The rewarming platform device mixes head motion, far infrared transmitter, cooling flow generating device, heads on again Regulation of the operation of warm fan, agitation fan and exhaust fan by the numerical control device.Wherein, bag or cell cryopreservation tube are frozen Resuscitation relevant information can with internet or local area network by wired or wireless networking, carry out data exchange, data storage and Data query.

A kind of working method of the anhydrous dry type recovery instrument of universal cell cryopreservation product, includes the following steps:

(1) start instrument, select recovery mode, open pre- heat function, the first emitter of far infrared and far infrared Second emitter starts to preheat, and after preheating, cell cryopreservation bag or cell cryopreservation tube are placed on to the elastic network(s) of rewarming rack On bottom, elastic square is covered, fixes cell cryopreservation bag or cell cryopreservation tube, covers instrument case lid, sends out far infrared second Injection device is placed exactly in the top of rewarming rack, and cell recovery starts, and rewarming rack carries out under the drive for mixing head motion Sticking up the formula of returning, back and forth mixing acts, and the first emitter of far infrared is emitting far-infrared, is radiated by the hollow part at elastic network(s) bottom Cell cryopreservation bag or cell cryopreservation tube are acted on, the second emitter of far infrared is emitting far-infrared, passes through elastic square Hollow part radiation effects is in cell cryopreservation bag or cell cryopreservation tube；

(2) itself meeting temperature increases at work for the first emitter of far infrared and the second emitter of far infrared, The head-on rewarming fan of heat ambient air, the first emitter of far infrared and far infrared the second emitter side is simultaneously It starts to work, head-on these warm heat air are head-on directly brushed cell cryopreservation bag or the cell in rewarming rack by rewarming fan Pipe surface is frozen, with the increase of face velocity, air side heat transfer coefficient be will increase, and add cell cryopreservation bag or cell cryopreservation tube Hot rewarming is more rapidly；

(3) agitation fan starts simultaneously at work, and agitation fan generates rough air, makes Air Temperature everywhere in instrument box It spends parameter and tends to identical, the intracorporal air themperature of air temperature sensor at-once monitor instrument container, detection data is transferred to numerical control Device, if instrument container is intracorporal, air temperature is too high, and more than the given threshold of specific rewarming mode, then numerical control device is controlled automatically Exhaust fan starting processed, the intracorporal hot-air of instrument container is discharged, while sucking the normal temperature air outside instrument box in time, is being stirred Under the action of fan, the normal temperature air of fresh sucking is mixed with the warm heat air that head-on rewarming fan brushes, and is reduced and is maintained The intracorporal air themperature of instrument container is in the fluctuation range of permission；

(4) resuscitative efforts mode has room temperature recovery and two kinds of high temperature rapid fluid resuscitation: under room temperature recovery mode, far infrared First emitter and the second emitter of far infrared work in a low-power state, and head-on rewarming fan generates head-on straight Blowing heating air-flow is warm wind, and brushing to the current of warm air temperature of cell cryopreservation bag or cell cryopreservation pipe surface is 37 DEG C~50 DEG C；? Under high temperature rapid fluid resuscitation mode, the first emitter of infrared ray and the second emitter of the far infrared work under high power state Make, the head-on blow-through heating air-flow that head-on rewarming fan generates is hot wind, is brushed to cell cryopreservation bag or cell cryopreservation pipe surface Hot stream temperature be 50 DEG C~70 DEG C；

(5) non-contact temperature sensor is frozen by the cell cryopreservation bag or cell placed in non-contact detection rewarming rack The infrared heat feature of pipe is deposited, and is converted and generates thermal image and temperature value, and then is shown over the display, and can be to temperature Angle value is calculated, and non-contact temperature sensor is provided simultaneously with visual identity function, can be according to freezing the outer of bag or cryopreservation tube Shape, precise positioning infrared heat monitoring point, the interference for avoiding other heat sources of maximum possible, in matching for AI intelligent numerical control software Under conjunction, detects cell cryopreservation bag or whether cell cryopreservation tube Current Temperatures match with the rewarming target temperature of setting, matching is stablized Determine that the time is not higher than 5 seconds, if being lower than rewarming target temperature, opens and keep heating mode, if being higher than rewarming target temperature, Then stop heating immediately, opens refrigeration mode rapidly；

(6) when being in heating mode, above the first emitter of far infrared and rewarming rack below rewarming rack The second emitter of far infrared is emitting far-infrared, by the hollow part radiation effects of elastic network(s) bottom and elastic square in cell Bag or cell cryopreservation tube are frozen, carries out rewarming using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, far The head-on rewarming fan of the first emitter of infrared ray and far infrared the second emitter side provides the warm heat for the blow-through that heads on Air stream, warm heat air stream are head-on directly brushed to cell cryopreservation bag or cell cryopreservation tube by the hollow part of elastic network(s) lid, are passed It passs heat and carries out rewarming；

(7) when non-contact temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether that cell freezes It deposits after the ice cube in bag or cell cryopreservation tube melts completely, then closes heating mode, open refrigeration mode, it is remote below rewarming rack The second emitter of far infrared below the first emitter of infrared ray and rewarming rack stops working, and far infrared first is sent out The head-on rewarming fan of injection device and far infrared the second emitter side is simultaneously stopped work, and the stopping of warm heat air stream is blown It strokes, while exhaust fan total power starts, the warm heat air in instrument box space, numerical control device intelligent control cooling flow is discharged Generating device is started to work, and is freezed by refrigeration machine or refrigeration source, and cooling fan starting is quickly converted to cold wind is brushed, freezes cell It deposits bag or cell cryopreservation tube cools down rapidly；

(8) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time, if do not taken away temporarily, Then refrigeration mode starts always, is run by numerical control device intelligent control, the automatic cell cryopreservation bag maintained in rewarming rack or The temperature of cell cryopreservation tube is conducive to keep cell viability at 0~5 DEG C.

(9) the resuscitation relevant information for freezing bag or cell cryopreservation tube can pass through wired or nothing with internet or local area network Line networking carries out data exchange, data storage and data query.

The working method of another universal anhydrous dry type recovery instrument of cell cryopreservation product, includes the following steps:

(1) start instrument, select recovery mode, open pre- heat function, double function heating pallets and far infrared transmitter Start to preheat, the temperature of double function heating pallets and far infrared transmitter starts to increase, and emitting far-infrared, and preheating terminates Afterwards, cell cryopreservation bag or cell cryopreservation tube are placed on double function heating pallets, cover fixed trampoline, fix cell cryopreservation bag or Cell cryopreservation tube covers instrument case lid, and cell recovery starts, and rewarming saddle carries out sticking up the formula of returning back and forth mixing movement on one side, on one side Pallet, which is heated, by double function passes through conduction heating and radiant heating two ways biography simultaneously to cell cryopreservation bag or cell cryopreservation tube Heat is passed, the far infrared transmitter in instrument case lid is emitting far-infrared, passes through the hollow part radiation effects of fixed trampoline In cell cryopreservation bag or cell cryopreservation tube；

(2) itself meeting temperature increases far infrared transmitter at work, heat ambient air, far infrared transmitting dress The head-on rewarming fan for setting side starts simultaneously at work, and head-on the gas collection of rewarming wind inlet of fan connection gas collection reflector is said Mouthful, the air of far infrared transmitter heating can be under the head-on suction of rewarming fan, the impeller clearance through gas collection reflector Into collection air flue, head-on the hollow part that these warm heat air pass through fixed trampoline is head-on directly brushed double function and added by rewarming fan Cell cryopreservation bag or cell cryopreservation pipe surface on hot rewarming apparatus, with the increase of face velocity, air side heat transfer coefficient meeting Increase, cell cryopreservation bag or cell cryopreservation tube is made to heat rewarming more rapidly；

(4) resuscitative efforts mode has room temperature recovery and two kinds of high temperature rapid fluid resuscitation: under room temperature recovery mode, double function heating The maximum operating temperature of pallet is 37 DEG C -39 DEG C, and far infrared transmitter works in a low-power state, head-on rewarming fan The head-on blow-through heating air-flow generated is warm wind, and brushing to the current of warm air temperature of cell cryopreservation bag or cell cryopreservation pipe surface is 37 DEG C~50 DEG C；Under high temperature rapid fluid resuscitation mode, the maximum temperature of double function heating pallets is 40 DEG C -70 DEG C, infrared ray transmitting dress It sets and works under high power state, the head-on blow-through heating air-flow that head-on rewarming fan generates is hot wind, is brushed to cell cryopreservation Bag or the hot stream temperature of cell cryopreservation pipe surface are 50 DEG C~70 DEG C；

(5) operating temperature of the double function heating pallets of temperature control temperature sensor real-time monitoring, detection data are transferred to numerical control dress It sets, numerical control device Intelligence Feedback regulates and controls the operating temperature of double function heating pallets in setting range, and non-contact temperature sensor is logical The infrared heat feature of non-contact detection cell cryopreservation bag or cell cryopreservation tube is crossed, detection data is transferred to numerical control device, and will It, which is converted, generates thermal image and temperature value, and then shows over the display, and can calculate temperature value, contactless temperature Degree sensor can be provided simultaneously with visual identity function, can be according to the shape for freezing bag or cryopreservation tube, precise positioning infrared heat prison Measuring point, the interference for avoiding other heat sources of maximum possible, under the cooperation of AI intelligent numerical control software, detect cell cryopreservation bag or Whether cell cryopreservation tube Current Temperatures match with the rewarming target temperature of setting, match and stablize the judgement time not higher than 5 seconds, if low In rewarming target temperature, then heating mode is opened and kept, if being higher than rewarming target temperature, stops heating immediately, open rapidly Open refrigeration mode；

(6) when being in heating mode, double function heating pallets are in heating state, and disk body temperature increases, and emits far infrared Line, heat is conducted through contact and radiation two ways is to cell cryopreservation bag or cell cryopreservation tube transmitting heat progress rewarming, meanwhile, Far infrared transmitter in instrument case lid is emitting far-infrared, is frozen by the hollow part radiation effects of fixed trampoline in cell Bag or cell cryopreservation tube are deposited, carries out rewarming using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, it is remote red The head-on rewarming fan of outside line emitter side provides the warm heat air stream for the blow-through that heads on, and warm heat air stream passes through fixed trampoline Hollow part head-on directly brush to cell cryopreservation bag or cell cryopreservation tube, transmit heat and carry out rewarming；

(7) when non-contact temperature sensor detects cell cryopreservation bag or cell cryopreservation tube temperature is more than 0 DEG C~8 DEG C, After can determine whether that the ice cube in cell cryopreservation bag or cell cryopreservation tube melts completely, then heating mode is closed, opens refrigeration mode, instrument The intracorporal double function heating rewarming apparatus of device case stop working, and the far infrared transmitter in instrument case lid stops working, remote red The head-on rewarming fan of outside line emitter side is simultaneously stopped work, and the stopping of warm heat air stream is brushed, while the full function of exhaust fan Rate starting, is discharged the warm heat air in work chamber upper space, and numerical control device intelligent control cooling flow generating device starts work Make, freezed by refrigeration machine or refrigeration source, cooling fan starting is quickly converted to cold wind is brushed, makes cell cryopreservation bag or cell cryopreservation Pipe cooling rapidly；

(8) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time, if do not taken away temporarily, Then refrigeration mode starts always, is run by numerical control device intelligent control, the automatic cell maintained in double function heating rewarming apparatus The temperature of bag or cell cryopreservation tube is frozen at 0~5 DEG C, is conducive to keep cell viability.

The present invention have the advantage that and the utility model has the advantages that

1, the present invention can be not only used for the anhydrous dry type recovery of cell cryopreservation bag, and can be used for the nothing of high-flux cell cryopreservation tube The recovery of water dry type.

2, the present invention by Far-infrared ray radiation heating, contact conduction heating and head-on warm heat air brush heating three kinds of rewarmings Two or three of combined application in mode, keeps the rewarming of cell cryopreservation bag or cell cryopreservation tube more efficient: rewarming platform device Using rewarming rack embodiment, Far-infrared ray radiation heating and head-on warm heat air are brushed into two kinds of rewarming modes of heating and combined Together；Rewarming platform device heats rewarming apparatus embodiment using double function, by Far-infrared ray radiation heating, contact conduction heating Head-on warm heat air brushes three kinds of rewarming modes of heating and combines.

3, rewarming platform device wants cell cryopreservation bag Cryopreservation Technology without heating plate using rewarming rack embodiment Ask not high, though cell cryopreservation bag freeze it is not smooth enough, nor affect on influence cell cryopreservation bag rewarming process.Rewarming rack With elastic network(s) bottom and elastic square, cell cryopreservation bag or cell cryopreservation tube can softly be pressurizeed and are fixed in rewarming rack, Rewarming rack stick up when the formula of returning back and forth mixes movement and will not be slided, and the elastic network(s) bottom of hollow out and elastic square both can permit far The first emitter of infrared ray and the far infrared of the second emitter of far infrared transmitting pass through elastic network(s) bottom and elastic square Hollow-out part be directly radiated to cell cryopreservation bag or cell cryopreservation pipe surface, and can make head-on blow-through heating air-flow or refrigeration gas Stream can directly brush cell cryopreservation bag or cell cryopreservation pipe surface by the hollow-out part at elastic network(s) bottom and elastic square, have Heat rapid rewarming is absorbed conducive to cell cryopreservation bag or cell cryopreservation tube or takes away heat fast cooling, the elastic network(s) bottom of hollow out Non-contact temperature sensor is not influenced also with elastic square to the cell cryopreservation bag or cell cryopreservation being fixed in rewarming rack The thermal imaging of pipe.

4, rewarming platform device heats rewarming apparatus embodiment using double function, and the use of fixed trampoline can be by cell cryopreservation Bag or cell cryopreservation tube, which softly pressurizes, to be fixed on double function heating pallets, rewarming saddle stick up the formula of returning back and forth mix move when It will not slide, the far infrared that the fixation trampoline of hollow out both can permit far infrared transmitter transmitting passes through fixed trampoline Hollow-out part is directly radiated to cell cryopreservation bag or cell cryopreservation pipe surface, and can make head-on blow-through heating air-flow or cooling flow Cell cryopreservation bag or cell cryopreservation pipe surface can be directly brushed by the hollow-out part of fixed trampoline, be conducive to cell cryopreservation Bag or cell cryopreservation tube absorb heat rapid rewarming or take away heat fast cooling, and the fixation trampoline of hollow out does not influence non-connect also Thermal imaging of the touch temperature sensor to cell cryopreservation bag or cell cryopreservation tube.

5, rewarming platform device heats rewarming apparatus embodiment using double function, and the use of isolation film is total together with rewarming saddle With by two spaced-aparts up and down of instrument box inner space, air does not circulate mutually, make the intracorporal upper space of instrument container into The dry type resuscitation of capable cell cryopreservation bag or cell cryopreservation tube does not interfere with the items of the intracorporal lower space of instrument container The operation of device.

6, rewarming platform device is placed in instrument box, can carry out sticking up the formula of returning back and forth mixing movement, built-in mixing work Mode is compared to the mixing operating mode of external, and it is not necessary that seal operation cabin is arranged on rewarming platform device, rewarming platform device is whole Body weight is light, and structure is simpler, is easy to technology realization, it is often more important that, built-in rewarming platform device facilitates the air system of outfit Cold function can be transferred to rapidly when to cell cryopreservation bag or cell cryopreservation tube selection high temperature rapid rewarming mode after rewarming Air cooling mode, is conducive to the holding of cell viability, and the preferred aluminum of material of the frame structure of rewarming platform device is light-weight.

7, the number of the servo motor of the mixing head motion of the rewarming platform device of four-point supporting type is 2 or 4, is utilized 4 eccentric wheels are flexibly connected with rewarming platform device by 4 supporting points by 4 connecting rods, and the operation of servo motor receives numerical control The regulation of device, by changing the revolving speed of servo motor and the starting angle of operation of eccentric wheel, the rewarming platform dress of four-point supporting type Setting may be implemented complicated and diversified stick up and returns formula reciprocating motion, and mixed effect is significantly stronger than the two o'clock branch of only 1 servo motor The mixed effect of support type seesaw type shaking table.

8, under high temperature recovery mode, the sticking up of rewarming platform device, which returns formula reciprocating motion, to be made in cell cryopreservation bag or cell cryopreservation tube Mixed effect is more preferable, and it is low the relative temperature of ice cube can be closed on inside by the high liquid of the relative temperature heated in periphery Liquid mixes in time, on the one hand cell is protected to be not affected by high temperatures and inactivate, on the other hand convenient for heat is rapid and uniform It takes to around ice cube, realizes that quick-thawing melts.

9, the rewarming fan that heads on is located at the side of the second emitter of the first emitter of far infrared and far infrared, far The first emitter of infrared ray and the second emitter of far infrared can generate high temperature at work, make neighbouring air themperature liter Height, head-on rewarming fan collects these heated air by the gas collection guide functions of gas collection reflector, and by these warm heats Air head-on directly brushes cell cryopreservation bag or cell cryopreservation pipe surface on rewarming platform device, can improve cell cryopreservation bag or The heated situation of cell cryopreservation tube makes cell cryopreservation bag or cell cryopreservation tube heat rewarming more rapidly, and more evenly, rewarming efficiency is more It is high.

10, independent refrigerating operaton module is arranged in cooling flow generating device, prestores cold source, once system detection is to cell It freezes bag or cell cryopreservation tube rewarming is completed, immediately intelligent starting cooling flow generating device, quick response, starting refrigeration wind Fan, be quickly converted makes the cooling of cell cryopreservation bag or cell cryopreservation tube more rapid to cold wind is brushed, moreover, refrigeration machine or low Warm storehouse both can be set to built-in, can also be also configured as the external of optional accessory, using it is more convenient flexibly.

11, non-contact temperature sensor is transferred to numerical control device by non-contact detection infrared heat, detection data, can It is converted and generates thermal image and temperature value, and then show over the display, and temperature value can be calculated, connect using non- Touch temperature sensor monitors the rewarming progress of cell cryopreservation tube or cell cryopreservation bag, can both avoid contact with formula temperature control spy Head sacrifices also contacts area and there are potential poor contacts to influence the drawbacks such as temperature control effect, and can be soft in AI intelligent numerical control The anhydrous dry type resuscitative efforts of competent high-flux cell cryopreservation tube under the cooperation of part.

12, it is provided with air temperature sensor in instrument box, to the more adaptable of working environment, the intelligence of complete machine Degree is higher, can command the rewarming fan that heads on, stirring by numerical control device feedback in time according to the intracorporal observed temperature of instrument container The operational mode of fan and exhaust fan maintains the intracorporal air themperature of instrument container in the fluctuation range that rewarming mode is set.

Detailed description of the invention

Fig. 1 is a kind of structure of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Schematic diagram；

Fig. 2 is a kind of rewarming of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Rack and the axis for mixing head motion survey overlooking structure diagram；

Fig. 3 is a kind of rewarming of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Rack and the axis for mixing head motion survey present invention looks up structural representation；

Fig. 4 is a kind of refrigeration of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Air-stream generating device structural schematic diagram；

Fig. 5 is a kind of gas collection of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Reflector and the head-on structural schematic diagram of rewarming fan；

Fig. 6 is a kind of entirety of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Structural schematic diagram；

Fig. 7 is a kind of double function of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention Heat rewarming apparatus structural schematic diagram；

Fig. 8 is a kind of double function of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention It heats rewarming apparatus axis and surveys bottom view；

Fig. 9 is a kind of the remote red of universal anhydrous dry type recovery instrument of cell cryopreservation product of one embodiment of the present invention The structural schematic diagram of outside line emitter, gas collection reflector and blow-through rewarming fan.

Specific embodiment

With reference to the accompanying drawings of the specification, the present invention is described in more detail.

Heating cells recovery is carried out using far infrared transmission device to cell cryopreservation bag or cell cryopreservation tube in the application to make Industry.

Far infrared transmitter is equipped with far infrared radiated element, including far-infrared heating plate and FAR INFRARED HEATING Pipe, can be emitting far-infrared after energization, and far infrared radiated element includes charcoal, carbon fiber product, silicon carbide, metal Pipe, resistance band, tourmaline, far-infrared ceramic, semiconductor, enamel, quartz ampoule, gold-plated quartz ampoule, devitrified glass lamp, metal oxidation Object, ruby heating tube, carbon fiber electric heating tube or far infrared oriented strong radiator.

According to the difference of the structure of the quantity of far infrared transmitter, installation site and rewarming platform device 3, be divided into as Lower embodiment:

Embodiment 1:

As shown in fig. 1, a kind of anhydrous dry type recovery instrument of universal cell cryopreservation product, comprising:

Instrument box 1 and instrument case lid 2, are flexibly connected between instrument box 1 and instrument case lid 2, and instrument case lid 2 can cover conjunction Or it opens on instrument box 1；

Far infrared transmitter is the first emitter of far infrared 4 and the second emitter of far infrared 6, far infrared The first emitter of line 4 is set in instrument box 1, and the second emitter of far infrared 6 is set in instrument case lid 2, far infrared First emitter 4 and the second emitter of far infrared 6 are respectively arranged on the two sides up and down of rewarming platform device 3；

It further include reflector 5 and gas collection reflector 7 except this, the first emitter of far infrared 4 is set to rewarming platform device 3 Lower section, reflector 5 is set to the lower section of the first emitter of far infrared 4, and the second emitter of far infrared 6 is installed on rewarming The top of platform device 3, gas collection reflector 7 are set to 6 top of the second emitter of far infrared.

Rewarming platform device 3, rewarming platform device 3 are mounted on inside instrument box 1, and rewarming platform device 3 is for placing cell jelly Bag or cell cryopreservation tube are deposited, the use scope of rewarming platform device 3 is wide, can be thin in recovery cell cryopreservation bag or cell cryopreservation tube Born of the same parents；Head motion 8 is mixed, mixes head motion 8 by being fixedly installed in 1 bottom of instrument box, and between rewarming platform device 3 It is connected by bolt；

Cooling flow generating device 13 is installed in 1 inside rear of instrument box；

Non-contact temperature sensor 14, is installed in 1 inside back upper place of instrument box, and non-contact temperature sensor 14 is logical It crosses bolt or is welded and fixed and be mounted in instrument box 1, the temperature monitoring direction of non-contact temperature sensor 14 is rewarming platform The upside or downside of device 3, there are Real Data Exchangs with numerical control device 15 for non-contact temperature sensor 14.

Among the above, non-contact temperature sensor 14 includes thermopile array non-contact temperature sensor, infrared measurement of temperature Probe temperature sensor, thermal infrared imager temperature sensor etc.；Non-contact temperature sensor is set to the intracorporal top of instrument container Or lower section, overall work face above or below rewarming rack can be carried out by the hollow part at elastic network(s) bottom and/or elastic square Wide-angle omni-directional temperature monitoring detects the infrared of the cell cryopreservation bag or cell cryopreservation tube placed in rewarming rack by non-contact Heat signature, and be converted and generate thermal image and temperature value, and then show over the display, and temperature value can be counted It calculates.The rewarming progress of cell cryopreservation tube or cell cryopreservation bag is monitored using non-contact temperature sensor, it both can be to avoid Contact temperature probe sacrifices also contacts area and influences the drawbacks such as temperature control effect there are potential poor contact, and can be The anhydrous dry type resuscitative efforts of competent high-flux cell cryopreservation tube under the cooperation of AI intelligent numerical control software.

As shown in Fig. 2, the rewarming platform device 3 of embodiment 1 is rewarming rack, rewarming rack includes rack frame 301, elastic network(s) Bottom 302, elastic square 303 are flexibly connected elastic square 303 on rack frame 301, and rack frame 301, which is flexibly connected, mixes shakeout machine Structure 8 mixes head motion 8 and is fixedly mounted on bottom in instrument box 1, and rack frame 301, which is movably arranged on, mixes head motion 8 Tache motorice, elastic network(s) bottom 302 is equipped in the middle part of rack frame 301, and the surrounding edge at elastic network(s) bottom 302 is connected with 301 inner edge of rack frame It connects, elasticated net bottom 302 is cross-woven by several warps and Ruo Kai weft, and elastic square 303 includes frame 3031 and elastic force Net 3032, frame 3031 are flexibly connected with rack frame 301, in the middle part of frame 3031 be equipped with elasticated net 3032, if elasticated net 3032 by Xeromenia line and Ruo Kai weft are cross-woven.301 material of rack frame is good thermal conductive metallic material, preferably aluminium, light weight, Intensity height, good heat conductivity.

Rewarming platform device 3 uses rewarming rack embodiment, without heating plate, to the requirement of cell cryopreservation bag Cryopreservation Technology It is not high, though cell cryopreservation bag freeze it is not smooth enough, nor affect on influence cell cryopreservation bag rewarming process.

Rewarming rack has elastic network(s) bottom 302 and elastic square 303, can softly add cell cryopreservation bag or cell cryopreservation tube Pressing is scheduled in rewarming rack, and stick up when the formula of returning back and forth mixes movement in rewarming rack will not slide, the elastic network(s) bottom of hollow out 302 and elastic square 303 both can permit the first emitter of far infrared 3 and the second emitter of far infrared 6 emits Far infrared is directly radiated to cell cryopreservation bag or cell cryopreservation by the hollow-out part at elastic network(s) bottom 302 and elastic square 303 Pipe surface, and the hollow out of head-on blow-through heating air-flow or cooling flow by elastic network(s) bottom 302 and elastic square 303 can be enable Cell cryopreservation bag or cell cryopreservation pipe surface are directly brushed in part, are conducive to cell cryopreservation bag or cell cryopreservation tube absorbs heat Rapid rewarming takes away heat fast cooling, and the elastic network(s) bottom 302 of hollow out and elastic square 303 do not influence contactless temperature also Spend thermal imaging of the sensor to the cell cryopreservation bag or cell cryopreservation tube that are fixed in rewarming rack.

Rewarming platform device 3 uses rewarming rack embodiment, makes Far-infrared ray radiation heating and head-on warm heat air brushes It heats two kinds of heating methods to match, the rewarming effect of cell cryopreservation bag or cell cryopreservation tube is more preferable.

As shown in figure 3, mixing head motion 8 includes power source 801, connecting rod 802 and bracket 803, power source 801 passes through Bolt or welding manner are fixedly connected on bottom in instrument box 1, and power source 801 includes servo motor 8011 and eccentric wheel 8012, the end of servo motor 8011 is equipped with eccentric wheel 8012, and the number of servo motor 8011 is 1~2, connecting rod 802 Number is 1~4, and the number of eccentric wheel 8012 is 1~4, connecting rod 802 by eccentric wheel 8012 and rack frame 301 by 1~ 4 tie points are flexibly connected, and the quantity of above-mentioned mentioned servo motor 8011, connecting rod 802 and eccentric wheel 8012 is according to reality Border use demand and select.

By the regulation of numerical control device 15, mixing head motion 8 can drive rewarming platform device 3 real for the operation of servo motor 8011 It now sticks up the formula of returning and back and forth mixes movement；Bracket 803 is flexibly connected rewarming platform device 3 by 2 supporting points, constitutes two o'clock support type Rewarming platform device 3, bracket 803 are flexibly connected rewarming platform device 3 by 4 supporting points, constitute the rewarming platform dress of four-point supporting type 3 are set, by changing the revolving speed of servo motor 8011 and the starting angle of operation of eccentric wheel 8012, the rewarming platform dress of two o'clock support type Set 3 or the rewarming platform device 3 of four-point supporting type the complicated and diversified formula of returning of sticking up may be implemented back and forth mix movement.

As shown in figure 4, cooling flow generating device 13 includes cold airflow spout 1301, cold water conservancy diversion rudder piece 1302, cold air road 1303, cooling fan 1304 and refrigeration source 1305, cold water conservancy diversion rudder piece 1302 are mounted on cold airflow spout 1301, cold airflow spout 1301 It is mounted on the air outlet in cold air road 1303, cold air road 1303 is connected to refrigeration source 1305, and refrigeration source 1305 connects with cooling fan 1304, Refrigeration source 1305 can be refrigeration machine or low temperature bin.

Above-mentioned, refrigeration machine can be built-in refrigeration machine or circumscribed refrigeration machine, and built-in refrigeration machine is located at universal cell It freezes inside the anhydrous dry type recovery instrument of product, circumscribed refrigeration machine is located at outside the anhydrous dry type recovery instrument of universal cell cryopreservation product Portion, refrigeration machine can be compression refrigerating machine, Absorption Refrigerator, steam jet refrigerator or semiconductor refrigerator；Low temperature bin Interior to place cryogenic substance, low temperature bin can be built-in low temperature bin or circumscribed low temperature bin, and built-in low temperature bin is set to universal Inside the anhydrous dry type recovery instrument of cell cryopreservation product, circumscribed low temperature bin is set to the universal anhydrous dry type of cell cryopreservation product Outside recovery instrument, cold airflow spout direction is rewarming platform device 3.

Compression refrigerating machine can be piston type, rotary, screw or centrifugal, and cryogenic substance can be dry ice, liquid nitrogen, pre- Freezing ice bag or ice cube, cooling flow generating device include regulating and controlling temperature and regulation wind speed function, and temperature control effect is more preferable, cooling flow Generating device can make instrument box internal temperature reduce rapidly, and be conducive to improve cell recovery motility rate.

As shown in figure 5, gas collection reflector 7 includes cover frame 701, internal layer reflection lobes 702, outer layer reflection lobes 703, internal layer Reflection lobes 702 be it is multiple, it is equidistant evenly distributed, be spaced each other distance 5mm~5cm, outer layer reflection lobes 703 be it is multiple, etc. Away from evenly distributed, it is spaced each other distance 5mm~5cm, internal layer reflection lobes 702 and outer layer reflection lobes 703 miss one another arrangement Setting, to collect air flue 54 between internal layer reflection lobes and cover, collection air flue 54 is communicated with impeller clearance, is collected the outlet of air flue 54 and is headed on The connection of 6 air inlet of rewarming fan, gas collection reflector 5 are set to 6 upside of the second emitter of far infrared, and far infrared second emits The far infrared that device 6 emits upwards can be reflected towards lower section through gas collection reflector 5, and the second emitter of far infrared 6 heats Air can be under the head-on power of rewarming fan 6, the impeller clearance through gas collection reflector 5 enters collection air flue 54, then through heading on The blow-through that rewarming fan 6 heads on heats on the upside of rewarming apparatus 3 to double function.

It further include head-on rewarming fan 9, agitation fan 10, exhaust fan 11 and air temperature sensor 12 in embodiment 1；It meets Face rewarming fan 9, agitation fan 10 and air temperature sensor 12 are mounted in instrument box 1, and exhaust fan 11 is mounted on instrument On 1 wall of device cabinet.

It further include numerical control device 15, DEU data exchange unit 16 and touch screen 17 except this, numerical control device 15 and data exchange fill It sets 16 to be mounted in instrument box 1, touch screen 17 is installed on instrument box 1.

Head-on rewarming fan 9 is mounted on the side of far infrared transmitter, and head-on the blowing direction of rewarming fan 9 is to meet Face blows to rewarming platform device 3, and head-on the number of rewarming fan 9 is 1~4；Agitation fan 10 is mountable in instrument box 1 Any position, the number of agitation fan 10 are 1~4；Exhaust fan 11 is installed on the tank wall of instrument box 1, the number of exhaust fan Mesh is 1~4；Head-on the start and stop of rewarming fan 9, agitation fan 10 and exhaust fan 11 receive the control of numerical control device 15 System；Head-on the quantity of rewarming fan 9, agitation fan 10 and exhaust fan 11 also according to actual needs depending on；Air temperature sensor 12 are installed on the different location in instrument box 1, and the number of air temperature sensor 12 is 1~4, air temperature sensor 12 There are Real Data Exchangs with numerical control device 15.

Head-on rewarming fan 9 is used to the warm heat air after the heating of far infrared injection device head-on directly brushing rewarming The cell cryopreservation bag or cell cryopreservation pipe surface of platform device 3；Agitation fan 10 is used for air themperature everywhere in instrument box 1 Parameter tends to identical；Air temperature sensor 12 is used for the intracorporal air themperature of at-once monitor instrument container；Agitation fan 10 generates Rough air makes air themperature parameter everywhere in instrument box 1 tend to identical；When air temperature sensor 12 monitors to specify When temperature range, numerical control device 15 can make corresponding control feedback according to real time temperature.

Embodiment 2:

As shown in fig. 6, a kind of anhydrous dry type recovery instrument of universal cell cryopreservation product, comprising:

Far infrared transmitter quantity is one, including far infrared transmitter 40 and gas collection reflector 70 are mounted on In instrument case lid 2, gas collection reflector 70 is mounted on 40 upside of far infrared transmitter；Rewarming platform device 3 is that double function add Hot rewarming apparatus, double function heating rewarming apparatus include rewarming saddle 31, double function heating pallet 32, isolation film 33, fixed trampoline 34 With mix head motion 8, fixed trampoline 34 is flexibly connected on rewarming saddle 31, rewarming saddle 31, which is flexibly connected, mixes head motion 8, it mixes head motion 8 and is fixedly mounted on bottom in instrument box 1, rewarming saddle 31, which is movably arranged on, mixes head motion 8 Tache motorice, 31 surrounding edge of rewarming saddle, which extends, is equipped with isolation film 33, and the upside of rewarming saddle 31 is pallet side, and pallet side is equipped with Double function heat pallet 32, and double function heating pallets 32 are flexibly connected with rewarming saddle 31, and double function heating pallets 32 are detachable and interchangeable, double Function heating pallet 32 is electrically connected with rewarming saddle 31 by piecing devices, the surrounding edge and 1 inner wall of instrument box of isolation film 33 Between be connected, rewarming saddle 31 and isolation film 33 are by two spaced-aparts up and down of 1 inner space of instrument box.

Rewarming platform device 3, rewarming platform device 3 are mounted on inside instrument box 1, and rewarming platform device 3 is for placing cell jelly Bag or cell cryopreservation tube are deposited, the use scope of rewarming platform device 3 is wide, can be thin in recovery cell cryopreservation bag or cell cryopreservation tube Born of the same parents；

Mix head motion 8, mix head motion 8 by being fixedly installed in 1 bottom of instrument box, and with rewarming platform device It is connected between 3 by bolt；

Among the above, non-contact temperature sensor 14 includes thermopile array non-contact temperature sensor, infrared measurement of temperature Probe temperature sensor, thermal infrared imager temperature sensor etc.；Non-contact temperature sensor is set to the intracorporal top of instrument container, Overall work face above rewarming apparatus can be heated to double function by the hollow part of fixed trampoline 34 and carry out wide-angle omni-directional temperature prison It surveys, passes through the non-contact infrared heat spy for detecting double function and heating the cell cryopreservation bag or cell cryopreservation tube placed in rewarming apparatus Sign, and be converted and generate thermal image and temperature value, and then show over the display, and temperature value can be calculated.It adopts The rewarming progress that cell cryopreservation tube or cell cryopreservation bag are monitored with non-contact temperature sensor, can both avoid contact with formula Temperature probe sacrifices also contacts area and there are potential poor contacts to influence the drawbacks such as temperature control effect, and can be in AI intelligence The anhydrous dry type resuscitative efforts of competent high-flux cell cryopreservation tube under the cooperation of numerical control software.

As shown in fig. 7, the rewarming platform device 3 of embodiment 2 is that double function heat rewarming apparatus, double function heating rewarming apparatus include Rewarming saddle 31, isolation film 33, fixes trampoline 34 at double function heating pallet 32, and fixed trampoline 34 is flexibly connected on rewarming saddle 31, Rewarming saddle 31, which is flexibly connected, mixes head motion 8, mixes head motion 8 and is fixedly mounted on bottom in instrument box 1, rewarming support Platform 31 is movably arranged on the tache motorice for mixing head motion 8, and 31 surrounding edge of rewarming saddle, which extends, is equipped with isolation film 33, rewarming support The upside of platform 31 is pallet side, and pallet side is equipped with double function and heats pallet 32, and double function heating pallets 32 and 31 activity of rewarming saddle are even It connects, double function heating pallets 32 are detachable and interchangeable, and double function heating pallets 32 are electrically connected with rewarming saddle 31 by piecing devices, double function Heating pallet 32 includes tray frame and calandria, and tray frame is made of metal, and calandria is set in tray frame, calandria To have the electric hot plate of two kinds of heating functions of conduction heating and Far-infrared ray radiation heating simultaneously, calandria is fixed with tray frame Connection, tray frame are equipped with temperature control temperature sensor, and temperature control temperature number of sensors is 1~6, the work of double function heating pallets 32 Making temperature range is 10 DEG C~70 DEG C, is painted with cell cryopreservation bag on double function heating pallets 32 or the placement position of cell cryopreservation tube refers to Conducting wire；It is connected between the surrounding edge of isolation film 33 and 1 inner wall of instrument box, rewarming saddle 31 and isolation film 33 are by instrument container Two spaced-aparts up and down of 1 inner space of body.

Rewarming platform device 3 heats rewarming apparatus embodiment using double function, and the use of fixed trampoline 34 can be by cell cryopreservation Bag or cell cryopreservation tube, which softly pressurizes, to be fixed on double function and heats on pallets, carries out sticking up the formula of returning back and forth mixing movement in rewarming saddle 31 Shi Buhui sliding, the far infrared that the fixation trampoline 34 of hollow out both can permit far infrared transmitter transmitting pass through fixed bullet The hollow-out part of net 34 is directly radiated to cell cryopreservation bag or cell cryopreservation pipe surface, and can make head-on blow-through heating air-flow or system Cold airflow can directly brush cell cryopreservation bag or cell cryopreservation pipe surface by the hollow-out part of fixed trampoline 34, be conducive to Cell cryopreservation bag or cell cryopreservation tube absorb heat rapid rewarming or take away heat fast cooling, and the fixation trampoline 34 of hollow out is also Temperature monitoring and thermal imaging of the non-contact temperature sensor to cell cryopreservation bag or cell cryopreservation tube are not influenced.

Rewarming platform device 3 uses double function to heat rewarming apparatus embodiments, adds Far-infrared ray radiation heating, contact conduction Heat and head-on warm heat air brush three kinds of heating methods of heating and match, and the rewarming effect of cell cryopreservation bag or cell cryopreservation tube is more It is good.

As shown in figure 8, mixing head motion 8 includes power source 801, connecting rod 802 and bracket 803, power source 801 passes through Bolt or welding manner are fixedly connected on bottom in instrument box 1, and power source 801 includes servo motor 8011 and eccentric wheel 8012, the end of servo motor 8011 is equipped with eccentric wheel 8012, and the number of servo motor 8011 is 1~2, connecting rod 802 Number is 1~4, and the number of eccentric wheel 8012 is 1~4, connecting rod 802 by eccentric wheel 8012 and rack frame 301 by 1~ 4 tie points are flexibly connected, and the quantity of above-mentioned mentioned servo motor 8011, connecting rod 802 and eccentric wheel 8012 is according to reality Border use demand and select.

The cooling flow generating device 13 of embodiment 2 includes cold airflow spout 1301, cold water conservancy diversion rudder piece 1302, cold air road 1303, cooling fan 1304 and refrigeration source 1305, cold water conservancy diversion rudder piece 1302 are mounted on cold airflow spout 1301, cold airflow spout 1301 It is mounted on the air outlet in cold air road 1303, cold air road 1303 is connected to refrigeration source 1305, and refrigeration source 1305 connects with cooling fan 1304, Refrigeration source 1305 can be refrigeration machine or low temperature bin.

Above-mentioned, refrigeration machine can be built-in refrigeration machine or circumscribed refrigeration machine, and built-in refrigeration machine is located at universal cell It freezes inside the anhydrous dry type recovery instrument of product, circumscribed refrigeration machine is located at outside the anhydrous dry type recovery instrument of universal cell cryopreservation product Portion, refrigeration machine can be compression refrigerating machine, Absorption Refrigerator, steam jet refrigerator or semiconductor refrigerator；Low temperature bin Interior to place cryogenic substance, low temperature bin can be built-in low temperature bin or circumscribed low temperature bin, and built-in low temperature bin is set to universal Inside the anhydrous dry type recovery instrument of cell cryopreservation product, circumscribed low temperature bin is set to the universal anhydrous dry type of cell cryopreservation product Outside recovery instrument, cold airflow spout direction is the rewarming platform device 3.

As shown in figure 9, it is in watt arc that gas collection reflector 70 is whole, including cover 71, outer layer reflection lobes 72, internal layer reflect Blade 73, outer layer reflection lobes 72 be it is multiple, it is equidistant evenly distributed, be spaced each other distance 5mm~5cm, internal layer reflection lobes 73 It is equidistant evenly distributed to be multiple, it is spaced each other distance 5mm~5cm, outer layer reflection lobes 72 is wrong each other with internal layer reflection lobes 73 Position is arranged, and is collection air flue 74 between internal layer reflection lobes 73 and cover 71, and collection air flue 74 is communicated with impeller clearance, collects air flue 74 Outlet is connected to head-on 9 air inlet of rewarming fan, and gas collection reflector 7 is located at 40 upside of far infrared transmitter, far infrared The far infrared that emitter 40 emits upwards can be reflected towards lower section through gas collection reflector 5, and far infrared transmitter 40 adds The air of heat can be under the head-on power of rewarming fan 9, and the impeller clearance through gas collection reflector 70 enters collection air flue 74, then through meeting The blow-through that face rewarming fan 9 heads on heats on the upside of rewarming platform device 3 to double function.

Embodiment 2 further includes head-on rewarming fan 9, agitation fan 10, exhaust fan 11 and air temperature sensor 12；Head-on Rewarming fan 9, agitation fan 10 and air temperature sensor 12 are mounted in instrument box 1, and exhaust fan 11 is mounted on instrument On 1 wall of cabinet.

The application method of a kind of anhydrous dry type recovery instrument of of the invention universal cell cryopreservation product and working method, including Following operating procedure:

1, power supply is opened, running parameter is set on touch screen, selects rewarming mode, machine preheating；

2, instrument case lid is opened, then opens the elastic square or fixed trampoline of rewarming platform device, cell to be recovered is frozen Deposit bag or cell cryopreservation tube be placed on rewarming platform device, then cover elastic square or fixed trampoline, close instrument case lid, then without Water dry type recovery instrument is started to work；

3, cell cryopreservation bag or cell cryopreservation tube are taken out after recovering, and shut instrument case lid；

4, after cell cryopreservation bag or cell cryopreservation tube rewarming, power supply is closed.

It is as follows based on above-mentioned anhydrous dry type recovery instrument:

In embodiment 1, a kind of working method of the universal anhydrous dry type recovery instrument of cell cryopreservation product, including walk as follows It is rapid:

1, start instrument, select recovery mode, open pre- heat function, the first emitter of far infrared and far infrared the Two emitters start to preheat, and after preheating, cell cryopreservation bag or cell cryopreservation tube are placed on to the elastic network(s) bottom of rewarming rack On, elastic square is covered, cell cryopreservation bag or cell cryopreservation tube are fixed, covers instrument case lid, emits far infrared second Device is placed exactly in the top of rewarming rack, and cell recovery starts, and rewarming rack is stuck up under the drive for mixing head motion Returning formula, back and forth mixing acts, and the first emitter of far infrared is emitting far-infrared, is radiated and is made by the hollow part at elastic network(s) bottom For cell cryopreservation bag or cell cryopreservation tube, the second emitter of far infrared is emitting far-infrared, passes through engraving for elastic square Vacancy radiation effects is in cell cryopreservation bag or cell cryopreservation tube；

2, itself meeting temperature increases at work for the first emitter of far infrared and the second emitter of far infrared, adds The head-on rewarming fan of hot surrounding air, the first emitter of far infrared and far infrared the second emitter side is opened simultaneously Beginning work, head-on these warm heat air are head-on directly brushed the cell cryopreservation bag or cell jelly in rewarming rack by rewarming fan Pipe surface is deposited, with the increase of face velocity, air side heat transfer coefficient be will increase, and heat cell cryopreservation bag or cell cryopreservation tube Rewarming is more rapidly；

3, agitation fan starts simultaneously at work, and agitation fan generates rough air, makes Air Temperature everywhere in instrument box It spends parameter and tends to identical, the intracorporal air themperature of air temperature sensor at-once monitor instrument container, detection data is transferred to numerical control Device, if instrument container is intracorporal, air temperature is too high, and more than the given threshold of specific rewarming mode, then numerical control device is controlled automatically Exhaust fan starting processed, the intracorporal hot-air of instrument container is discharged, while sucking the normal temperature air outside instrument box in time, is being stirred Under the action of fan, the normal temperature air of fresh sucking is mixed with the warm heat air that head-on rewarming fan brushes, and is reduced and is maintained The intracorporal air themperature of instrument container is in the fluctuation range of permission；

4, resuscitative efforts mode has room temperature recovery and two kinds of high temperature rapid fluid resuscitation: under room temperature recovery mode, far infrared First emitter and the second emitter of far infrared work in a low-power state, and head-on rewarming fan generates head-on straight Blowing heating air-flow is warm wind, and brushing to the current of warm air temperature of cell cryopreservation bag or cell cryopreservation pipe surface is 37 DEG C~50 DEG C；? Under high temperature rapid fluid resuscitation mode, the first emitter of infrared ray and the second emitter of the far infrared work under high power state Make, the head-on blow-through heating air-flow that head-on rewarming fan generates is hot wind, is brushed to cell cryopreservation bag or cell cryopreservation pipe surface Hot stream temperature be 50 DEG C~70 DEG C；

5, non-contact temperature sensor is frozen by the cell cryopreservation bag or cell placed in non-contact detection rewarming rack The infrared heat feature of pipe is deposited, and is converted and generates thermal image and temperature value, and then is shown over the display, and can be to temperature Angle value is calculated, and non-contact temperature sensor is provided simultaneously with visual identity function, can be according to freezing the outer of bag or cryopreservation tube Shape, precise positioning infrared heat monitoring point, the interference for avoiding other heat sources of maximum possible, in matching for AI intelligent numerical control software Under conjunction, detects cell cryopreservation bag or whether cell cryopreservation tube Current Temperatures match with the rewarming target temperature of setting, matching is stablized Determine that the time is not higher than 5 seconds, if being lower than rewarming target temperature, opens and keep heating mode, if being higher than rewarming target temperature, Then stop heating immediately, opens refrigeration mode rapidly；

6, remote above the first emitter of far infrared and rewarming rack below rewarming rack when being in heating mode The second emitter of infrared ray is emitting far-infrared, is frozen by the hollow part radiation effects at elastic network(s) bottom and elastic square in cell Bag or cell cryopreservation tube are deposited, carries out rewarming using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, it is remote red The warm heat that the head-on rewarming fan of the first emitter of outside line and far infrared the second emitter side provides the blow-through that heads on is empty Air-flow, warm heat air stream are head-on directly brushed by the hollow part of elastic network(s) lid to cell cryopreservation bag or cell cryopreservation tube, transmitting Heat carries out rewarming；

7, when non-contact temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether cell cryopreservation After ice cube in bag or cell cryopreservation tube melts completely, then heating mode is closed, open refrigeration mode, rewarming rack lower section is remote red The second emitter of far infrared below the first emitter of outside line and rewarming rack stops working, and far infrared first emits The head-on rewarming fan of device and far infrared the second emitter side is simultaneously stopped work, and the stopping of warm heat air stream is brushed, The warm heat air in instrument box space, numerical control device intelligent control cooling flow hair is discharged in exhaust fan total power starting simultaneously Generating apparatus is started to work, and is freezed by refrigeration machine or refrigeration source, and cooling fan starting is quickly converted to cold wind is brushed, makes cell cryopreservation Bag or cell cryopreservation tube cool down rapidly；

8, the cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time, if do not taken away temporarily, Then refrigeration mode starts always, is run by numerical control device intelligent control, the automatic cell cryopreservation bag maintained in rewarming rack or The temperature of cell cryopreservation tube is conducive to keep cell viability at 0~5 DEG C.

9, the resuscitation relevant information for freezing bag or cell cryopreservation tube can pass through wired or nothing with internet or local area network Line networking carries out data exchange, data storage and data query.

In embodiment 2, the working method of another universal anhydrous dry type recovery instrument of cell cryopreservation product, wherein including Following steps:

1, start instrument, select recovery mode, open pre- heat function, double function heating pallets and far infrared transmitter are opened Begin to preheat, the temperature of double function heating pallets and far infrared transmitter starts to increase, and emitting far-infrared, and preheating terminates Afterwards, cell cryopreservation bag or cell cryopreservation tube are placed on double function heating pallets, cover fixed trampoline, fix cell cryopreservation bag or Cell cryopreservation tube covers instrument case lid, and cell recovery starts, and rewarming saddle carries out sticking up the formula of returning back and forth mixing movement on one side, on one side Pallet, which is heated, by double function passes through conduction heating and radiant heating two ways biography simultaneously to cell cryopreservation bag or cell cryopreservation tube Heat is passed, the far infrared transmitter in instrument case lid is emitting far-infrared, passes through the hollow part radiation effects of fixed trampoline In cell cryopreservation bag or cell cryopreservation tube；

2, itself meeting temperature increases far infrared transmitter at work, heat ambient air, far infrared transmitting dress The head-on rewarming fan for setting side starts simultaneously at work, and head-on the gas collection of rewarming wind inlet of fan connection gas collection reflector is said Mouthful, the air of far infrared transmitter heating can be under the head-on suction of rewarming fan, the impeller clearance through gas collection reflector Into collection air flue, head-on the hollow part that these warm heat air pass through fixed trampoline is head-on directly brushed double function and added by rewarming fan Cell cryopreservation bag or cell cryopreservation pipe surface on hot rewarming apparatus, with the increase of face velocity, air side heat transfer coefficient meeting Increase, cell cryopreservation bag or cell cryopreservation tube is made to heat rewarming more rapidly；

4, resuscitative efforts mode has room temperature recovery and two kinds of high temperature rapid fluid resuscitation: under room temperature recovery mode, double function heating The maximum operating temperature of pallet is 37 DEG C -39 DEG C, and far infrared transmitter works in a low-power state, head-on rewarming fan The head-on blow-through heating air-flow generated is warm wind, and brushing to the current of warm air temperature of cell cryopreservation bag or cell cryopreservation pipe surface is 37 DEG C~50 DEG C；Under high temperature rapid fluid resuscitation mode, the maximum temperature of double function heating pallets is 40 DEG C -70 DEG C, infrared ray transmitting dress It sets and works under high power state, the head-on blow-through heating air-flow that head-on rewarming fan generates is hot wind, is brushed to cell cryopreservation Bag or the hot stream temperature of cell cryopreservation pipe surface are 50 DEG C~70 DEG C；

5, the operating temperature of the double function heating pallets of temperature control temperature sensor real-time monitoring, detection data are transferred to numerical control dress It sets, numerical control device Intelligence Feedback regulates and controls the operating temperature of double function heating pallets in setting range, and non-contact temperature sensor is logical The infrared heat feature of non-contact detection cell cryopreservation bag or cell cryopreservation tube is crossed, detection data is transferred to numerical control device, and will It, which is converted, generates thermal image and temperature value, and then shows over the display, and can calculate temperature value, contactless temperature Degree sensor can be provided simultaneously with visual identity function, can be according to the shape for freezing bag or cryopreservation tube, precise positioning infrared heat prison Measuring point, the interference for avoiding other heat sources of maximum possible, under the cooperation of AI intelligent numerical control software, detect cell cryopreservation bag or Whether cell cryopreservation tube Current Temperatures match with the rewarming target temperature of setting, match and stablize the judgement time not higher than 5 seconds, if low In rewarming target temperature, then heating mode is opened and kept, if being higher than rewarming target temperature, stops heating immediately, open rapidly Open refrigeration mode；

6, when being in heating mode, double function heating pallets are in heating state, and disk body temperature increases, and emits far infrared Line, heat is conducted through contact and radiation two ways is to cell cryopreservation bag or cell cryopreservation tube transmitting heat progress rewarming, meanwhile, Far infrared transmitter in instrument case lid is emitting far-infrared, is frozen by the hollow part radiation effects of fixed trampoline in cell Bag or cell cryopreservation tube are deposited, carries out rewarming using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, it is remote red The head-on rewarming fan of outside line emitter side provides the warm heat air stream for the blow-through that heads on, and warm heat air stream passes through fixed trampoline Hollow part head-on directly brush to cell cryopreservation bag or cell cryopreservation tube, transmit heat and carry out rewarming；

It 7, can when non-contact temperature sensor detects cell cryopreservation bag or cell cryopreservation tube temperature is more than 0 DEG C~8 DEG C After judging that the ice cube in cell cryopreservation bag or cell cryopreservation tube melts completely, then heating mode is closed, opens refrigeration mode, instrument The intracorporal double function heating rewarming apparatus of case stop working, and the far infrared transmitter in instrument case lid stops working, far infrared The head-on rewarming fan of line emitter side is simultaneously stopped work, and the stopping of warm heat air stream is brushed, while exhaust fan total power The warm heat air in work chamber upper space is discharged in starting, and numerical control device intelligent control cooling flow generating device is started to work, Freezed by refrigeration machine or refrigeration source, cooling fan starting is quickly converted to cold wind is brushed, keeps cell cryopreservation bag or cell cryopreservation tube fast Prompt drop temperature；

8, the cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time, if do not taken away temporarily, Then refrigeration mode starts always, is run by numerical control device intelligent control, the automatic cell maintained in double function heating rewarming apparatus The temperature of bag or cell cryopreservation tube is frozen at 0~5 DEG C, is conducive to keep cell viability.

The above is only preferred embodiment of the invention, it is noted that those skilled in the art, not Under the premise of being detached from the invention design, some similar deformation and improvement can be made, these are also considered as invention protection Within.

Claims

1. a kind of anhydrous dry type recovery instrument of universal cell cryopreservation product, it is characterised in that: including

Instrument box (1) and instrument case lid (2), the instrument box (1) are flexibly connected with the instrument case lid (2)；

It is internal to be installed on the instrument box (1) for far infrared transmitter；

Rewarming platform device (3), the rewarming platform device (3) are mounted on the instrument box (1) inside, and rewarming platform device (3) is used In placement cell cryopreservation bag or cell cryopreservation tube；

Mix head motion (8), the mixing head motion (8) is installed in the instrument box (1) bottom, and with the rewarming Platform device (3) is connected；

Cooling flow generating device (13), it is internal that the cooling flow generating device (13) is installed in the instrument box (1)；

Non-contact temperature sensor (14), the non-contact temperature sensor (14) are installed in the instrument box (1) Portion.

2. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1, it is characterised in that: described Far infrared transmitter is equipped with far infrared radiated element, including far-infrared heating plate and far-infrared heating tube, is powered After can be emitting far-infrared, the far infrared radiated element includes charcoal, carbon fiber product, silicon carbide, metal tube, electricity It is stopband, tourmaline, far-infrared ceramic, semiconductor, enamel, quartz ampoule, gold-plated quartz ampoule, devitrified glass lamp, metal oxide, red Jewel heating tube, carbon fiber electric heating tube or far infrared oriented strong radiator.

3. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 2, it is characterised in that: described Far infrared transmitter is the first emitter of far infrared (4) and the second emitter of far infrared (6), the far infrared The first emitter of line (4) is set in the instrument box (1), and second emitter of far infrared (6) is set to the instrument In device case lid (2), first emitter of far infrared (4) and the second emitter of the far infrared (6) are respectively arranged on The two sides up and down of the rewarming platform device (3)；The far infrared transmitter further includes reflector (5) and gas collection reflector (7), first emitter of far infrared (4) is set to the lower section of the rewarming platform device (3), and the reflector (5) is set to The lower section of first emitter of far infrared (4), second emitter of far infrared (6) are installed on the rewarming platform The top of device (3), the gas collection reflector (7) are set to above second emitter of far infrared (6).

4. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 3, it is characterised in that: described anti- Penetrate cover (5) and the gas collection reflector (7) integrally in watt arc, the gas collection reflector (7) includes that cover frame (701), internal layer are anti- Penetrate blade (702), outer layer reflection lobes (703), the internal layer reflection lobes (702) be it is multiple, it is equidistant evenly distributed, to each other Gauge from 5mm~5cm, the outer layer reflection lobes (703) be it is multiple, it is equidistant evenly distributed, be spaced each other distance 5mm~5cm, The internal layer reflection lobes (702) misses one another with the outer layer reflection lobes (703) and is arranged, the internal layer reflection lobes It is collection air flue (54) between the cover, the collection air flue (54) communicates with impeller clearance, collection air flue (54) outlet and institute Head-on rewarming fan (6) air inlet connection is stated, the gas collection reflector (5) is set on the upside of the second emitter of far infrared (6), The far infrared that second emitter of far infrared (6) emits upwards can be reflected towards down through the gas collection reflector (5) The air of side, the second emitter of far infrared (6) heating can be under the head-on power of rewarming fan (6), through the collection The impeller clearance of gas reflector (5) enters the collection air flue (54), then head on blow-through Xiang Shuangong through head-on rewarming fan (6) It heats on the upside of rewarming apparatus (3).

5. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 4, it is characterised in that: described Rewarming platform device (3) is rewarming rack, and the rewarming rack includes rack frame (301), elastic network(s) bottom (302) and elastic square (303), the elastic square (303) is flexibly connected on the rack frame (301), described in the rack frame (301) is flexibly connected It mixes head motion (8), the mixing head motion (8) is fixedly mounted on the instrument box (1) interior bottom, the rack frame (301) it is movably arranged on the tache motorice for mixing head motion (8), is equipped with the elastic network(s) in the middle part of the rack frame (301) Bottom (302), the surrounding edge of the elastic network(s) bottom (302) are connected with rack frame (301) inner edge, the elasticated net bottom (302) it is cross-woven by several warps and several wefts, the elasticity square (303) includes frame (3031) and elasticated net (3032), the frame (3031) is flexibly connected with the rack frame (301), and the elastic force is equipped in the middle part of the frame (3031) Net (3032), the elasticated net (3032) are cross-woven by several warps and Ruo Kai weft.

6. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 2, it is characterised in that: described Far infrared transmitter quantity is one, including far infrared transmitter (40) and gas collection reflector (70) are mounted on instrument In case lid (2), the gas collection reflector (70) is mounted on the upside of the far infrared transmitter (40)；The rewarming platform device (3) rewarming apparatus are heated for double function, double function heating rewarming apparatus include rewarming saddle (31), double function heating pallet (32), Isolation film (33) fixes trampoline (34), and fixed trampoline (34), the rewarming saddle are flexibly connected on the rewarming saddle (31) (31) it is flexibly connected the mixing head motion (8), the mixing head motion (8) is fixedly mounted on the instrument box (1) Interior bottom, the rewarming saddle (31) are movably arranged on the tache motorice for mixing head motion (8), the rewarming saddle (31) Surrounding edge, which extends, is equipped with the isolation film (33), and the upside of the rewarming saddle (31) is pallet side, and pallet side is equipped with described Double function heat pallet (32), and double function heating pallet (32) are flexibly connected with the rewarming saddle (31), double function heating Pallet (32) is detachable and interchangeable, and double function heating pallet (32) are electrically connected with the rewarming saddle (31) by piecing devices, Double function heating pallet (32) include tray frame and calandria, and the tray frame is made of metal, and the calandria is set In in the tray frame, the calandria is while having two kinds of heating functions of conduction heating and Far-infrared ray radiation heating Electric hot plate, the calandria are fixedly connected with the tray frame, and the tray frame is equipped with temperature control temperature sensor, the temperature Controlling temperature number of sensors is 1~6, and the operating temperature range of double function heating pallet (32) is 10 DEG C~70 DEG C, described The placement position guide line of cell cryopreservation bag or cell cryopreservation tube is painted on double function heating pallet (32)；The isolation film (33) It is connected between surrounding edge and the instrument box (1) inner wall, the rewarming saddle (31) and the isolation film (33) are by institute State two spaced-aparts up and down of instrument box (1) inner space.

7. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1, it is characterised in that: described Mixing head motion (8) includes power source (801), connecting rod (802) and bracket (803), and the power source (801) is fixedly connected In the instrument box (1) interior bottom, the power source (801) includes servo motor (8011) and eccentric wheel (8012), described The both ends of servo motor (8011) are equipped with the eccentric wheel (8012), and the number of the servo motor (8011) is 1~2, institute The number for stating connecting rod (802) is 1~4, and the number of the eccentric wheel (8012) is 1~4, and the connecting rod (802) will The eccentric wheel (8012) is flexibly connected with the rewarming platform device (3) by 1~4 tie point, the servo motor (8011) by the regulation of the numerical control device (15), the mixing head motion (8) can drive the rewarming platform device for operation (3) realize that sticking up the formula of returning back and forth mixes movement.

8. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1, it is characterised in that: described Cooling flow generating device (13) includes cold airflow spout (1301), cold water conservancy diversion rudder piece (1302), cold air road (1303), cooling fan (1304) and refrigeration source (1305), the cold water conservancy diversion rudder piece (1302) are mounted on the cold airflow spout (1301), the cold air Stream spout (1301) is mounted on the air outlet of the cold air road (1303), and the cold air road (1303) is connected to the refrigeration source (1305), the refrigeration source (1305) connects with the cooling fan (1304), and the refrigeration source (1305) can be refrigeration machine or low Wen Cang can place cryogenic substance in the low temperature bin.

9. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1, it is characterised in that: described Non-contact temperature sensor (14) is fixedly mounted in the instrument box (1), the non-contact temperature sensor (14) Temperature monitoring direction be the rewarming platform device (3) upside or downside, the non-contact temperature sensor (14) and institute Stating numerical control device (15), there are Real Data Exchangs, and the non-contact temperature sensor (14) includes that thermopile array is non-contact Formula temperature sensor, infrared temperature probe temperature sensor, thermal infrared imager temperature sensor etc..

10. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1, it is characterised in that: also Including the rewarming fan (9) that heads on, agitation fan (10) and air temperature sensor (12)；Head-on rewarming fan (9), stirring Fan (10) and air temperature sensor (12) are mounted in instrument box (1), and described head-on rewarming fan (9) are for will be through Warm heat air after the heating of far infrared injection device head-on directly brushes the cell cryopreservation bag of rewarming platform device (3) or cell freezes Deposit pipe surface, the agitation fan (10) is used to air themperature parameter everywhere in instrument box (1) tending to identical, the sky Gas temperature sensor (12) is used for the intracorporal air themperature of at-once monitor instrument container.

11. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 10, it is characterised in that: institute State the side that the far infrared transmitter is arranged in head-on rewarming fan (9), the blowing side of head-on rewarming fan (9) To head-on to blow to the rewarming platform device (3), the number of head-on rewarming fan (9) is 1~4；The agitation fan (10) it is set to any position in the instrument box (1), the number of the agitation fan (10) is 1~4；The exhaust fan (11) it is set on the tank wall of the instrument box (1), the number of the exhaust fan is 1~4；The head-on rewarming fan (9), the start and stop of agitation fan (10) and exhaust fan (11) receive the control of the numerical control device (15)；The air Temperature sensor (12) is set to the different location in the instrument box (1), and the number of the air temperature sensor (12) is 1 ~4, there are Real Data Exchangs for the air temperature sensor (12) and the numerical control device (15).

12. a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 10, it is characterised in that: also Including exhaust fan (11), numerical control device (15), DEU data exchange unit (16) and touch screen (17), the exhaust fan (11) is mounted on On instrument box (1) wall, the numerical control device (15) and the DEU data exchange unit (16) are mounted on the instrument box (1) in, the touch screen (17) are installed on the instrument box (1)；

The rewarming platform device (3), mix head motion (8), far infrared transmitter, cooling flow generating device (13), Head-on the operation of rewarming fan (9), agitation fan (10) and exhaust fan (11) is by the regulation of the numerical control device (15).

13. based on a kind of universal anhydrous dry type of cell cryopreservation product described in any one of Claims 1 to 5 and 7~12 The working method of recovery instrument, wherein include the following steps:

(1) start instrument, select recovery mode, open pre- heat function, the first emitter of far infrared and far infrared second Emitter starts to preheat, and after preheating, cell cryopreservation bag or cell cryopreservation tube are placed on the elastic network(s) bottom of rewarming rack, Elastic square is covered, cell cryopreservation bag or cell cryopreservation tube are fixed, covers instrument case lid, rewarming rack is mixing head motion Drive under carry out seesaw type back and forth mixing act, cell recovery starts；

(2) the first emitter of far infrared and the second emitter of far infrared are started to work, the head-on rewarming fan of side Start simultaneously at work；

(3) agitation fan starts simultaneously at work, and agitation fan generates rough air, makes air themperature ginseng everywhere in instrument box Number tends to identical, and the intracorporal air themperature of air temperature sensor at-once monitor instrument container, detection data is transferred to numerical control device, If instrument container is intracorporal, air temperature is too high, and more than the given threshold of specific rewarming mode, then numerical control device automatically controls row The intracorporal hot-air of instrument container is discharged in time, while sucking the normal temperature air outside instrument box by starting fan, in agitation fan Under the action of, the normal temperature air of fresh sucking is mixed with the warm heat air that head-on rewarming fan brushes, and is reduced and is maintained instrument The intracorporal air themperature of case is in the fluctuation range of permission；

(4) resuscitative efforts mode has room temperature recovery and two kinds of high temperature rapid fluid resuscitation；

(5) cell cryopreservation bag or cell cryopreservation tube that non-contact temperature sensor passes through placement in non-contact detection rewarming rack Infrared heat feature, detection data is transferred to numerical control device, and is converted and generates thermal image and temperature value, and then be shown in On display, and temperature value can be calculated；

(6) remote red above the first emitter of far infrared and rewarming rack below rewarming rack when being in heating mode The second emitter of outside line is emitting far-infrared, by the hollow part radiation effects of elastic network(s) bottom and elastic square in cell cryopreservation Bag or cell cryopreservation tube carry out rewarming using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, far infrared The head-on rewarming fan of the first emitter of line and far infrared the second emitter side provides the warm heat air for the blow-through that heads on Stream, warm heat air stream are head-on directly brushed by the hollow part of elastic network(s) lid to cell cryopreservation bag or cell cryopreservation tube, transmitting heat Amount carries out rewarming；

(7) it when non-contact temperature sensor detects cell cryopreservation bag or cryopreservation tube temperature is more than 0 DEG C~8 DEG C, can determine whether thin Born of the same parents freeze after the ice cube in bag or cell cryopreservation tube melts completely, then close heating mode, open refrigeration mode, under rewarming rack The second emitter of far infrared below square the first emitter of far infrared and rewarming rack stops working, far infrared The head-on rewarming fan of one emitter and far infrared the second emitter side is simultaneously stopped work, and warm heat air stream stops It brushes, while exhaust fan total power starts, the warm heat air in instrument box space, numerical control device intelligent control refrigeration gas is discharged Flow generating apparatus is started to work, and is freezed by refrigeration machine or refrigeration source, and cooling fan starting is quickly converted to cold wind is brushed, makes cell It freezes bag or cell cryopreservation tube cools down rapidly；

(8) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed.

14. based on a kind of universal anhydrous dry type of cell cryopreservation product described in any one of claim 1~2 and 6~12 The working method of recovery instrument, wherein include the following steps:

(1) start instrument, select recovery mode, open pre- heat function, double function heating pallets and far infrared transmitter start Preheating after preheating, cell cryopreservation bag or cell cryopreservation tube is placed on double function heating pallets, fixed trampoline is covered, fixed Good cell cryopreservation bag or cell cryopreservation tube, cover instrument case lid, and cell recovery starts, and rewarming saddle carries out sticking up the formula of returning on one side reciprocal Mixing movement heats pallet by double function on one side and passes through contact conduction and radiation two simultaneously to cell cryopreservation bag or cell cryopreservation tube Kind mode transmits heat, and the far infrared transmitter in instrument case lid is emitting far-infrared, passes through the hollow part of fixed trampoline Radiant heating acts on cell cryopreservation bag or cell cryopreservation tube；

(2) itself meeting temperature increases far infrared transmitter at work, heat ambient air, by far infrared transmitter The head-on rewarming fan of side starts simultaneously at work；

(5) operating temperature of the double function heating pallets of temperature control temperature sensor real-time monitoring, detection data are transferred to numerical control device, number It controls device Intelligence Feedback and regulates and controls pair operating temperature of function heating pallets in setting range, non-contact temperature sensor is connect by non- Feeler inspection surveys the infrared heat feature of cell cryopreservation bag or cell cryopreservation tube, and detection data is transferred to numerical control device, and is converted Thermal image and temperature value are generated, and then is shown over the display, and temperature value can be calculated；

(6) when being in heating mode, double function heating pallets are in heating state, and disk body temperature increases, and emitting far-infrared, heat Amount is conducted through contact and radiation two ways transmits heat to cell cryopreservation bag or cell cryopreservation tube and carries out rewarming, meanwhile, instrument Far infrared transmitter in case lid is emitting far-infrared, by the hollow part radiation effects of fixed trampoline in cell cryopreservation bag Or cell cryopreservation tube, rewarming is carried out using Far-infrared ray radiation heating cell cryopreservation bag or cell cryopreservation tube, meanwhile, far infrared The head-on rewarming fan of emitter side provides the warm heat air stream for the blow-through that heads on, and warm heat air stream passes through engraving for fixed trampoline Vacancy is head-on directly brushed to cell cryopreservation bag or cell cryopreservation tube, is transmitted heat and is carried out rewarming；

(7) when non-contact temperature sensor detects cell cryopreservation bag or cell cryopreservation tube temperature is more than 0 DEG C~8 DEG C, can sentence After ice cube in disconnected cell cryopreservation bag or cell cryopreservation tube melts completely, then heating mode is closed, opens refrigeration mode, instrument container Intracorporal double function heating rewarming apparatus stop working, and the far infrared transmitter in instrument case lid stops working, far infrared The head-on rewarming fan of emitter side is simultaneously stopped work, and the stopping of warm heat air stream is brushed, while exhaust fan total power opens It is dynamic, the warm heat air in work chamber upper space is discharged, numerical control device intelligent control cooling flow generating device is started to work, by Refrigeration machine or refrigeration source refrigeration, cooling fan starting are quickly converted to cold wind is brushed, keep cell cryopreservation bag or cell cryopreservation tube rapid Cooling；

15. the work of described in any item a kind of universal anhydrous dry type recovery instrument of cell cryopreservation product according to claim 1~12 Make method, wherein freeze bag or cell cryopreservation tube resuscitation relevant information can with internet or local area network by wired or Wireless Networking carries out data exchange, data storage and data query.