CN106995782A - A kind of dry type cell recovery instrument - Google Patents
A kind of dry type cell recovery instrument Download PDFInfo
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- CN106995782A CN106995782A CN201710271936.1A CN201710271936A CN106995782A CN 106995782 A CN106995782 A CN 106995782A CN 201710271936 A CN201710271936 A CN 201710271936A CN 106995782 A CN106995782 A CN 106995782A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
This application discloses a kind of dry type cell recovery instrument, including function cabin, function cabin includes nacelle and can be covered on the hatchcover of nacelle；Nacelle is provided with heat conduction pallet and the first heating element heater that can be heated to heat conduction pallet；Hatchcover is provided with heat conducting cover plate and the second heating element heater that can be heated to heat conducting cover plate；When hatchcover is covered on nacelle, working chamber is collectively forming between heat conducting cover plate and heat conduction pallet；Vibrating motor is provided with nacelle, nacelle bottom is provided with resilient support portion.Thus, dry type heating is carried out by heating element heater, pallet and cover plate that heat-conducting medium material is made is recycled to carry out heat conduction, cell work chamber is collectively forming between heat conducting cover plate and heat conduction pallet and carries out cell recovery, to replace traditional water bath device, using dry type cell recovery, it is to avoid the pollution risk that water-bath cell recovery is brought；Cell cryopreservation bag or cell cryopreservation tube concussion can also be made, the liquid of thawing is mixed in time, be heated evenly.
Description
Technical field
The present invention relates to Biomedical Instruments, more particularly to a kind of dry type cell recovery instrument.
Background technology
It is liquid nitrogen frozen to the important way that cell biological resource is preserved in cell biology, i.e., by cell
Freeze in liquid nitrogen container, when experiment needs, further take out freeze-stored cell and recovered.In cell recovery, optimum operation be by
The cell cryopreservation tube that liquid nitrogen container has just taken out is rapidly heated to 37 DEG C, and it is most easily damaged -5~0 DEG C to be allowed to run through cell, with
Avoid ice crystal from recrystallizing to cell damage, cause the death of cell.
In current practical operation, experimenter grips 2~4 cell cryopreservation tubes by tweezers, in common water-bath
Make cell quick-thawing by acutely rocking, but this ordinary operation mode is difficult the resuscitative efforts for completing high-volume cell；
There is company by adding single pallet in water-bath, realize the way for many solencytes that thaw simultaneously；But it is due to that cell freezes
Deposit self-temperature when pipe takes out out of liquid nitrogen just to spend more than subzero 100, while when many solencytes are put into water-bath, necessarily making
Water temperature into the region does not reach 37 DEG C in a long time, compared with conventional method defrosting single tube cell, extends cell recovery
Time, violate the principle of cell " freezing slowly instant ", increase repeats the risk of crystallization, reduces cytoactive.Water-bath is recovered
Cell also increases the possibility of cell contamination, it is impossible to meet cell produce, freeze, recovery whole process sterileization operation require.
In addition, traditional cell recovery still needs artificial operation resuscitation process, it is impossible to ensure the correct time of recovery；And
The change of resuscitator temperature can not be monitored in real time, it is impossible to ensure the accurate temperature of recovery.
The content of the invention
The purpose of the present invention is to be to provide a kind of dry type cell recovery instrument, solves one in above-mentioned prior art problem
Or it is multiple.
According to an aspect of the present invention there is provided a kind of dry type cell recovery instrument, including function cabin, function cabin includes nacelle
With can be covered on the hatchcover of nacelle；Nacelle is provided with heat conduction pallet and the first heating unit that can be heated to heat conduction pallet
Part；Hatchcover is provided with heat conducting cover plate and the second heating element heater that can be heated to heat conducting cover plate；When hatchcover is covered on nacelle,
Working chamber is collectively forming between heat conducting cover plate and heat conduction pallet；Vibrating motor is provided with nacelle, nacelle bottom is provided with resilient support
Portion.
The present invention carries out dry type heating by heating element heater, recycles pallet and cover plate that heat-conducting medium material is made to carry out
Heat conduction, is collectively forming cell work chamber and carries out cell recovery between heat conducting cover plate and heat conduction pallet, to replace traditional water-bath to fill
Put, using dry type cell recovery, it is to avoid the pollution risk that water-bath cell recovery is brought；Also by setting vibrating motor and elasticity
Supporting part, can realize horizontal circumference concussion function, and vibrating motor drives function cabin to do horizontal circumference concussion, and resilient support portion is carried
The effect of high function cabin vibrations, then drives the cell cryopreservation bag in working chamber or cell cryopreservation tube concussion, makes the liquid of thawing
Mix, be heated evenly in time.
In some embodiments：Resilient support portion is four, and is respectively arranged on four corners of nacelle, resilient support portion
Including spring and sucker, spring one end is fixedly connected on nacelle, and the other end is fixedly connected on sucker.Thus, sucker can be by work(
Energy cabin is fixed, and spring can coordinate vibrating motor function cabin is done horizontal circumference concussion.
In some embodiments：First heating element heater and the second heating element heater are Peltier, graphene Electric radiant Heating Film or pottery
Porcelain heating board.Thereby, it is possible to provide dry type heating, pollution risk will not be brought to cell recovery.
In some embodiments：The one side that heat conduction pallet is located at working chamber is plane, and heat conducting cover plate is located at working chamber
One side be plane.Thus, it is possible to carry out cell recovery to cell cryopreservation bag.
In some embodiments：Heat conduction pallet is located at the one side of working chamber provided with multiple the first half arc flutings, heat conduction
Cover plate is located at the one side of working chamber provided with multiple the second half arc flutings that tube chamber is cooperatively formed with the first half arc flutings.Thus, it is possible to
It is enough that cell recovery is carried out to cell cryopreservation tube.
In some embodiments：Working chamber includes freezing bag working chamber and cryopreservation tube working chamber；Heat conduction pallet, which is located at, to be frozen
The one side for depositing bag working chamber is plane, and heat conducting cover plate, which is located at, freezes the one side of bag working chamber for plane；Heat conduction pallet is located at
The one side of cryopreservation tube working chamber is provided with multiple the first half arc flutings, and the one side that heat conducting cover plate is located at cryopreservation tube working chamber is provided with
Multiple the second half arc flutings that tube chamber is cooperatively formed with the first half arc flutings.Thus, cell can be carried out to cell cryopreservation bag to answer
Soviet Union, can carry out cell recovery to cell cryopreservation tube again.
In some embodiments：The one side that heat conducting cover plate is located at working chamber is provided with heat conductive silica gel.Thus, heat conductive silica gel
The 3D shape of cell cryopreservation bag upper surface will be voluntarily adapted to, make it between heat conducting cover plate completely without air gap, can
Carry out uniform quick heating.
In some embodiments：The one side that heat conducting cover plate is located at working chamber is provided with heat conductive silica gel；The of heat conduction pallet
Half arc fluting surface is provided with heat conductive silica gel.Thus, heat conductive silica gel will voluntarily adapt to cell cryopreservation bag or cell cryopreservation pipe surface
3D shape, make its respectively between heat conducting cover plate and heat conduction pallet completely without air gap, can carry out uniform fast
Speed heating.
In some embodiments：Also include controller and at least one temperature sensor, heat conduction pallet and heat conducting cover plate
Be equipped with temperature sensor, temperature sensor is used to measuring the temperature for being positioned over article in working chamber, temperature sensor with control
Device electrical connection processed, controller can control the open and close of the first heating element heater, the second heating element heater and vibrating motor；Data
Interface can be installed on pedestal, and data line interface can network and downloading data.Thus, temperature sensor is used for monitoring intracavitary
Cell cryopreservation bag or cell cryopreservation tube temperature, and the temperature signal is sent to controller, controller is believed according to the temperature
Number control the open and close of the first heating element heater, the second heating element heater and vibrating motor.
In some embodiments：Also include indicator lamp and siren, indicator lamp and siren are electrically connected with the controller,
Controller can control indicator lamp and siren.When the work of function cabin is completed, it can be carried out by indicator lamp and siren
Remind.
In some embodiments：Also include touch screen and data-interface, touch screen and data-interface are electric with controller
Connection, can select mode of operation and working procedure, input control information, output data etc. by touch screen.
In some embodiments：The first convection space is provided between heat conduction pallet and nacelle bottom, cabin body sidewall is provided with
The second convection space, hatchcover top are provided between at the top of the first passage communicated with the first convection space, heat conducting cover plate and hatchcover
Portion is provided with the second passage communicated with the second convection space.Thus, when working chamber needs to start to cool or cool down, first pair
Fluid space, the second convection space, the first passage and the second passage can be to working chamber, the first heating element heater and the second heating
Element carries out quick heat radiating cooling.
In some embodiments：Vibrating motor includes motor and is arranged at the eccentric wheel of motor output end, turn of motor
Son is vertically installed in nacelle.Thus, during startup, the circular motion of eccentric wheel causes mass centre changing, drives function cabin to do horizontal circle
Week concussion.
Brief description of the drawings
Fig. 1 is a kind of structural representation of dry type cell recovery instrument of the invention；
Fig. 2 is that a kind of heat conduction pallet of dry type cell recovery instrument of the invention and the structure of first embodiment of heat conducting cover plate are shown
It is intended to；
Fig. 3 is that a kind of heat conduction pallet of dry type cell recovery instrument of the invention and the structure of second embodiment of heat conducting cover plate are shown
It is intended to；
Fig. 4 is that a kind of heat conduction pallet of dry type cell recovery instrument of the invention and the structure of 3rd embodiment of heat conducting cover plate are shown
It is intended to；
Fig. 5 is a kind of first embodiment structural representation of the controller setting of dry type cell recovery instrument of the invention；
Fig. 6 is a kind of second embodiment structural representation of the controller setting of dry type cell recovery instrument of the invention.
Embodiment
Illustrate below in conjunction with the accompanying drawings, the present invention is described in further detail.
As shown in figure 1, a kind of dry type cell recovery instrument, including function cabin, function cabin is including nacelle 1 and can be covered on cabin
The hatchcover 2 of body 1, specifically, the side of hatchcover 2 are articulated with the side of nacelle 1.Nacelle 1 is provided with heat conduction pallet 11 and can be right
The first heating element heater 12 that heat conduction pallet 11 is heated, specifically, heat conduction pallet 11 is arranged at the inside of nacelle 1, heat conduction
The edge of pallet 11 is fixedly connected on the inwall of nacelle 1, and the first heating element heater 12 is attached at the back side of heat conduction pallet 11, heat conduction
The back side of pallet 11 is that heat conduction pallet 11 is metal tray in the present embodiment, it is preferred that be metal towards the side of nacelle 1
Aluminium is made, and can carry out efficient heat transfer.Hatchcover 2 adds provided with heat conducting cover plate 21 with can be heated to heat conducting cover plate 21 second
Thermal element 23, heat conducting cover plate 21 is fixedly installed in hatchcover 2, and the second heating element heater 23 is attached at the back side of heat conducting cover plate 21, heat conduction
The back side of cover plate 21 is that in the present embodiment, heat conducting cover plate 21 is metal cover board, it is preferred that be metal towards the side of hatchcover 2
Aluminium is made, being capable of efficient heat transfer.When hatchcover 2 is covered on nacelle 1, work is collectively forming between heat conducting cover plate 21 and heat conduction pallet 11
Make chamber 4, working chamber 4 can be used for placing cell cryopreservation bag or cell cryopreservation tube carries out cell recovery.Provided with vibration electricity in nacelle 1
Machine 16, vibrating motor 16 can be fixedly installed in the bottom of heat conduction pallet 11, and vibrating motor 16 can drive function cabin to do level
Circumference shakes, in the present embodiment, and vibrating motor 16 can include motor and be arranged at the eccentric wheel of motor output end, and motor is solid
Dingan County is loaded on the bottom of nacelle 1, and the rotors normal of motor is arranged at function cabin.Thus, during startup, the circular motion of eccentric wheel draws
Mass centre changing is played, drives function cabin to do horizontal circumference concussion.The bottom of nacelle 1 is provided with resilient support portion, for coordinating vibration
Motor 16, improves vibrating effect.Thus, dry type heating is carried out by heating element heater, the support for recycling heat-conducting medium material to be made
Disk and cover plate carry out heat conduction, and cell work chamber is collectively forming between heat conducting cover plate and heat conduction pallet and carries out cell recovery, to replace
Traditional water bath device, using dry type cell recovery, it is to avoid the pollution risk that water-bath cell recovery is brought；Also shaken by setting
Dynamic motor and resilient support portion, realize the horizontal circumference concussion motion of nacelle, then drive cell cryopreservation bag in working chamber or
Cell cryopreservation tube shakes, and the liquid of thawing is mixed in time, is heated evenly.
Wherein, resilient support portion is four, and is respectively arranged on four corners of nacelle 1, and resilient support portion includes the He of spring 5
Sucker 6, one end of spring 5 is fixedly connected on nacelle 1, and the other end is fixedly connected on sucker 6.Thus, sucker can enter function cabin
Row is fixed, and spring can coordinate vibrating motor function cabin is done horizontal circumference concussion.
Wherein, the first heating element heater 12 and the second heating element heater 23 are Peltier, graphene Electric radiant Heating Film or ceramic heating plate.
Thereby, it is possible to provide dry type heating, pollution risk will not be brought to cell recovery.In the present embodiment, the first heating element heater 12
It is Peltier with the second heating element heater 23, wherein, Peltier is semiconductor chilling plate, is also thermoelectric module, is a kind of heat
Pump, it is not necessary to any refrigerant, can continuously work, and not shaken and noise during work, long lifespan.Semiconductor chilling plate has two
Function is planted, can be freezed, and can be heated, therefore discrete heating system and refrigeration system can be just replaced using an element.
Semiconductor chilling plate is electric current transducing type element, by the control of input current, and high-precision temperature control can be achieved, adds
Temperature detection and microprocessor control device, it is easy to constitute automatic control system.Semiconductor chilling plate thermal inertia is very small, system
It is cold to heat the time quickly, in the case where the good cold end of hot-side heat dissipation is unloaded, it is powered less than one minute, cooling piece is with regard to that can reach most
The big temperature difference.
Embodiment 1, as shown in Fig. 2 the one side that heat conduction pallet 11 is located at working chamber 4 is plane, heat conducting cover plate 21 is located at
The one side of working chamber 4 is plane.Thus, it is possible to carry out cell recovery to cell cryopreservation bag.
Embodiment 2, as shown in figure 3, heat conduction pallet 11 is located at the one side of working chamber 4 provided with multiple the first half arc flutings,
It is recessed provided with multiple the second half arcs that tube chamber is cooperatively formed with the first half arc flutings that heat conducting cover plate 21 is located at the one side of working chamber 4
Groove.Thus, it is possible to carry out cell recovery to cell cryopreservation tube.
Embodiment 3, as shown in figure 4, working chamber 4 includes freezing bag working chamber and cryopreservation tube working chamber；11, heat conduction pallet
In freezing the one side of bag working chamber for plane, heat conducting cover plate 21, which is located at, freezes the one side of bag working chamber for plane；Heat conduction support
Disk 11 is located at the one side of cryopreservation tube working chamber provided with multiple the first half arc flutings, and heat conducting cover plate 21 is located at cryopreservation tube working chamber
One side is provided with multiple the second half arc flutings that tube chamber is cooperatively formed with the first half arc flutings.Thus, can be to cell cryopreservation bag
Cell recovery is carried out, cell recovery can be carried out to cell cryopreservation tube again.
Wherein, in above-described embodiment 1, heat conducting cover plate 21 can be sticked positioned at the one side of working chamber 4 thermal conductive silicon
Glue 24.Thus, heat conductive silica gel will voluntarily adapt to the 3D shape of cell cryopreservation bag upper surface, make it complete between heat conducting cover plate
There is no air gap, can uniformly be heated.In above-described embodiment 2 and 3, heat conducting cover plate 21 is located at the side of working chamber 4
Face, which is sticked, heat conductive silica gel 24；The first half arc fluting surfaces of heat conduction pallet 11 are sticked and have heat conductive silica gel 24.Thus, heat conductive silica gel
The 3D shape of cell cryopreservation bag or cell cryopreservation pipe surface will voluntarily be adapted to, make its respectively with heat conducting cover plate and heat conduction pallet it
Between completely without air gap, uniform quick heating can be carried out.
In addition, being provided with the first convection space 13 between heat conduction pallet 11 and the bottom of nacelle 1, the side wall of nacelle 1 is offered and the
The first passage (not shown) that one convection space 13 is communicated, in the present embodiment, is provided with dividing plate (not shown) in nacelle 1, every
Form the installation portion of vibrating motor 16 between plate and the bottom of nacelle 1, the first convection space 13 be located at heat conduction pallet 11 and dividing plate it
Between.The second convection space 22 is provided between heat conducting cover plate 21 and the top of hatchcover 2, the top of hatchcover 2 is offered and the second convection space
22 the second passage (not shown) communicated.Thus, when working chamber needs to start to cool or cool down, the first convection space, the
Two convection spaces, the first passage and the second passage can be carried out to working chamber, the first heating element heater and the second heating element heater
Quick heat radiating cools.
In order to realize intelligent control, controller 17 and at least one temperature sensor 4, the He of heat conduction pallet 11 can also be included
Heat conducting cover plate 21 is equipped with temperature sensor 4, and temperature sensor 4 is used to measure the temperature for being positioned over article in working chamber 4, specifically
For, the probe of temperature sensor 4 can be located in working chamber 4, and temperature sensor 4 is electrically connected with controller 17, controller
17 can control the open and close of the first heating element heater 12, the second heating element heater 23 and vibrating motor 16.Thus, TEMP
Device is used for the cell cryopreservation bag of monitoring intracavitary or the temperature of cell cryopreservation tube, and the temperature signal is sent into controller,
Controller controls the open and close of the first heating element heater, the second heating element heater and vibrating motor according to the temperature signal.
In addition, it can include siren 15, siren 15 can be installed on the outer wall of nacelle 1, siren 15 is electrically connected
Controller 17 is connected to, siren is installed on pedestal 1.Thus, when cell recovery is completed, alarm can be carried out by siren 15 and carried
Wake up.Indicator lamp 14 can also be included, indicator lamp 14 can be installed on the outer wall of nacelle 1, and indicator lamp 14 is electrically connected to controller
17, indicator lamp 14 can be installed on pedestal 1.Thus, when cell recovery is completed, it can be reminded by indicator lamp.Can be with
Including touch screen 18 and data-interface, touch screen 18 and data-interface are electrically connected to controller 17, and touch screen 18 is used to set
The running parameters such as heating-up temperature, vibrating motor rotating speed, data-interface can be installed on the side wall of nacelle 1, and data-interface can be with
Carry out data transmission.
Wherein, as shown in figure 5, being also provided with housing 7, controller 17 and touch screen 18 are installed on housing 7, controller
17 can pass through the first heating element heater in wire linkage function cabin, the second heating element heater, vibrating motor, temperature sensor, police
Report device and indicator lamp.In addition, as shown in fig. 6, can also include pedestal 9, pedestal 9 can substitute sucker 6, that is to say, that spring 5
One end is fixedly connected on nacelle 1, and the other end is fixedly connected on pedestal 9, and controller 17 and touch screen 18 can be installed on pedestal 9
On, controller 17 can pass through the first heating element heater in wire linkage function cabin, the second heating element heater, vibrating motor, temperature
Sensor, siren, data-interface and indicator lamp.Thus, it is possible to so that overall structure advantages of simple.
When in use, this dry type cell recovery instrument is positioned over to smooth operating desk surface first, consolidated by sucker 6
It is fixed, then switch on power, operated on touch screen 18, the running parameter such as setting heating-up temperature, vibrating motor rotating speed preheats heat conduction
Cell cryopreservation tube or cell cryopreservation bag are placed in the heat conduction pallet 11 of function cabin 2 by pallet 11 and heat conducting cover plate 21 to design temperature
On, close the three-dimensional that the heat conductive silica gel 24 on hatchcover 2, hatchcover 2 will voluntarily adapt to cell cryopreservation tube or cell cryopreservation bag upper surface
Shape, makes it between heat conducting cover plate 21 completely without air gap.Then cell recovery is carried out, controller 17 sends control letter
Number, the second heating element heater 23 quick heat production simultaneously on the first heating element heater 12 and hatchcover 2 in control nacelle 2 passes through heat conduction
Pallet 11, heat conducting cover plate 21 and heat conductive silica gel 24 transfer heat to cell cryopreservation tube or cell cryopreservation bag.Controller 17 is simultaneously
Control signal is sent to vibrating motor 16, vibrating motor 16 starts running, drive function cabin vibrations, spring 5 can increase function
The vibrating effect in cabin, drives function cabin 2 to do horizontal circumference concussion, then drives cell cryopreservation tube or cell in working chamber 4 to freeze
Bag concussion is deposited, the liquid of thawing is mixed in time, is heated evenly.Liquid thawing in cell cryopreservation tube or cell cryopreservation bag,
When temperature increases to over 0~4 DEG C, temperature sensor 4 detects the temperature change of cell cryopreservation tube or cell cryopreservation bag, by signal
Controller 17 is passed to, negative-feedback signal is sent, makes the second heating unit on the first heating element heater 12 and the hatchcover 2 in nacelle 2
Part 23 terminates heating, and vibrating motor 16 shuts down, and being simultaneously emitted by control information makes indicator lamp 14 light, and makes siren sounding,
Teaching process person's cell recovery terminates, and cell cryopreservation tube or cell cryopreservation bag is now taken out in time, you can carry out next step operation.
Or when temperature sensor 4 detect cell cryopreservation tube or cell cryopreservation bag temperature meet or exceed 0~4 DEG C when, immediately will
Signal feeds back to controller 17, sends negative-feedback signal, vibrating motor 16 is shut down, the first heating element heater in nacelle 2
12 and hatchcover 2 on the second heating element heater 23 terminate heating, switch to refrigeration mode, cool the temperature to and maintain 4 DEG C.Send out simultaneously
Going out control information makes indicator lamp 14 light, and makes siren sounding, teaching process person's cell recovery terminates, and cell is now taken out in time
Cryopreservation tube or cell cryopreservation bag, you can carry out next step operation.
Described above is only one embodiment of the present invention, it is noted that to those skilled in the art,
Without departing from the concept of the premise of the invention, some similar modification and improvement can also be made, these also should be regarded as this
Within the protection domain of invention.
Claims (13)
1. a kind of dry type cell recovery instrument, it is characterised in that：Including function cabin, the function cabin includes nacelle (1) and can covered
Together in the hatchcover (2) of the nacelle (1)；The nacelle (1) is provided with heat conduction pallet (11) and can be to the heat conduction pallet (11)
The first heating element heater (12) heated；The hatchcover (2) is provided with heat conducting cover plate (21) and can be to the heat conducting cover plate
(21) the second heating element heater (23) heated；When the hatchcover (2) is covered on the nacelle (1), the heat conducting cover plate
(21) working chamber (4) is collectively forming between the heat conduction pallet (11)；Vibrating motor (16), institute are provided with the nacelle (1)
Nacelle (1) bottom is stated provided with resilient support portion.
2. a kind of dry type cell recovery instrument according to claim 1, it is characterised in that：The resilient support portion is four,
And four corners of the nacelle (1) are respectively arranged on, the resilient support portion includes spring (5) and sucker (6), the spring
(5) one end is fixedly connected on the nacelle (1), and the other end is fixedly connected on the sucker (6).
3. a kind of dry type cell recovery instrument according to claim 1, it is characterised in that：First heating element heater (12) and
Second heating element heater (23) is Peltier, graphene Electric radiant Heating Film or ceramic heating plate.
4. a kind of dry type cell recovery instrument according to claim 3, it is characterised in that：The heat conduction pallet (11) is located at institute
The one side of working chamber (4) is stated provided with multiple the first half arc flutings, the heat conducting cover plate (21) is located at the one of the working chamber (4)
Side is provided with multiple the second half arc flutings that tube chamber is cooperatively formed with the first half arc fluting.
5. a kind of dry type cell recovery instrument according to claim 3, it is characterised in that：The heat conduction pallet (11) is located at institute
The one side for stating working chamber (4) is plane, and the one side that the heat conducting cover plate (21) is located at the working chamber (4) is plane.
6. a kind of dry type cell recovery instrument according to claim 3, it is characterised in that：The working chamber (4) includes freezing
Bag working chamber and cryopreservation tube working chamber；It is plane, institute that the heat conduction pallet (11), which is located at the one side for freezing bag working chamber,
It is plane to state heat conducting cover plate (21) to be located at the one side for freezing bag working chamber；The heat conduction pallet (11) is located at described freeze
The one side of pipe working chamber is located at the one of the cryopreservation tube working chamber provided with multiple the first half arc flutings, the heat conducting cover plate (21)
Side is provided with multiple the second half arc flutings that tube chamber is cooperatively formed with the first half arc fluting.
7. a kind of dry type cell recovery instrument according to any one of claim 5, it is characterised in that：The heat conducting cover plate (21)
One side positioned at the working chamber (4) is provided with heat conductive silica gel (24).
8. a kind of dry type cell recovery instrument according to any one of claim 4 or 6, it is characterised in that：The heat conducting cover plate
(21) one side positioned at the working chamber (4) is provided with heat conductive silica gel (24)；The first half arc flutings of the heat conduction pallet (11)
Surface is provided with heat conductive silica gel (24).
9. a kind of dry type cell recovery instrument according to claim 1, it is characterised in that：Also including controller (17) and at least
One temperature sensor (3), the heat conduction pallet (11) and the heat conducting cover plate (21) are equipped with the temperature sensor (3),
The temperature sensor (3) is used for the temperature that measurement is positioned over the working chamber (4) interior article, and the temperature sensor (3) is
Electrically connected with the controller (17), the controller (17) can control first heating element heater (12), described second to add
The open and close of thermal element (23) and the vibrating motor (16).
10. a kind of dry type cell recovery instrument according to claim 9, it is characterised in that：Also include siren (15) and refer to
Show lamp (14), the indicator lamp (14) and siren (15) are electrically connected with the controller (17), controller (17) energy
Enough control the indicator lamp (14) and siren (15).
11. a kind of dry type cell recovery instrument according to claim 9, it is characterised in that：Also include touch screen (18) sum
According to interface, the touch screen (18) and data-interface are electrically connected with the controller (17), and the touch screen (18) can be defeated
Enter and output information.
12. a kind of dry type cell recovery instrument according to claim 1, it is characterised in that：The heat conduction pallet (11) and institute
State and the first convection space (13) is provided between nacelle (1) bottom, nacelle (1) side wall is provided with and first convection space
(13) the second convection space is provided between at the top of the first passage communicated, the heat conducting cover plate (21) and the hatchcover (2)
(22), provided with the second passage communicated with second convection space (22) at the top of the hatchcover (2).
13. a kind of dry type cell recovery instrument according to claim 1, it is characterised in that：The vibrating motor (16) includes
Motor and the eccentric wheel for being arranged at the motor output end, the rotors normal of the motor are arranged at the nacelle (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710271936.1A CN106995782A (en) | 2017-04-24 | 2017-04-24 | A kind of dry type cell recovery instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710271936.1A CN106995782A (en) | 2017-04-24 | 2017-04-24 | A kind of dry type cell recovery instrument |
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| CN106995782A true CN106995782A (en) | 2017-08-01 |
Family
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|---|---|---|---|
| CN201710271936.1A Pending CN106995782A (en) | 2017-04-24 | 2017-04-24 | A kind of dry type cell recovery instrument |
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| CN107365700A (en) * | 2017-09-06 | 2017-11-21 | 银丰生物工程集团有限公司 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
| CN107746799A (en) * | 2017-11-21 | 2018-03-02 | 赛慕特生物工程（上海）有限公司 | A kind of hand-held dry type cell recovery instrument |
| CN107881101A (en) * | 2017-11-21 | 2018-04-06 | 赛慕特生物工程（上海）有限公司 | One kind rubs formula cell cryopreservation bag dry type recovery instrument |
| CN108260587A (en) * | 2018-01-15 | 2018-07-10 | 赛慕特生物工程（上海）有限公司 | It a kind of large capacity cell cryopreservation tube and its mating freezes and dry type recovery device |
| CN109059418A (en) * | 2018-09-15 | 2018-12-21 | 乔燕春 | A kind of biological products freeze and recovery device and freeze and method for resuscitation |
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| CN110157599A (en) * | 2019-07-10 | 2019-08-23 | 陕西中医药大学 | A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells |
| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
| CN110305786A (en) * | 2019-06-20 | 2019-10-08 | 大连理工大学 | A kind of hand-held, can be from the dry type cell resuscitator of disinfection |
| CN113491797A (en) * | 2021-07-29 | 2021-10-12 | 青岛海尔生物医疗科技有限公司 | Vibration control method of dry type slurry melting machine |
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| CN107365700A (en) * | 2017-09-06 | 2017-11-21 | 银丰生物工程集团有限公司 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
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| CN108260587A (en) * | 2018-01-15 | 2018-07-10 | 赛慕特生物工程（上海）有限公司 | It a kind of large capacity cell cryopreservation tube and its mating freezes and dry type recovery device |
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| CN109059418A (en) * | 2018-09-15 | 2018-12-21 | 乔燕春 | A kind of biological products freeze and recovery device and freeze and method for resuscitation |
| CN109609347A (en) * | 2019-02-15 | 2019-04-12 | 赛慕特生物工程（上海）有限公司 | A kind of cell cryopreservation bag dry type recovery device and working method |
| CN109609347B (en) * | 2019-02-15 | 2024-04-16 | 江苏赛拉纳生物医药有限公司 | Dry-type resuscitator for cell cryopreservation bag and working method |
| CN110093267A (en) * | 2019-05-31 | 2019-08-06 | 江苏普瑞康生物医药科技有限公司 | A kind of cell recovery instrument |
| CN110305786A (en) * | 2019-06-20 | 2019-10-08 | 大连理工大学 | A kind of hand-held, can be from the dry type cell resuscitator of disinfection |
| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
| CN110157599A (en) * | 2019-07-10 | 2019-08-23 | 陕西中医药大学 | A kind of culture device of pilot scale culture umbilical cord mesenchymal stem cells |
| CN113491797A (en) * | 2021-07-29 | 2021-10-12 | 青岛海尔生物医疗科技有限公司 | Vibration control method of dry type slurry melting machine |
| CN114410468A (en) * | 2022-01-26 | 2022-04-29 | 衷博瑞佳生物技术(上海)有限公司 | High-efficient biological cell recovery equipment |
| CN114410468B (en) * | 2022-01-26 | 2024-05-28 | 衷博瑞佳生物技术(上海)有限公司 | High-efficient biological cell resuscitator |
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Effective date of registration: 20171026 Address after: 200000 Shanghai Free Trade Zone Branch Road No. 58 Building 2 Xiang B zone 2 room 209 Applicant after: Saimute Biological Engineering (Shanghai) Co., Ltd. Address before: 226000 3 West Port, nine port, Gangzha District, Jiangsu, Nantong Applicant before: He Xiangfeng Applicant before: Wang Xiaojun |