CN107365700A - A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal - Google Patents
A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal Download PDFInfo
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- CN107365700A CN107365700A CN201710796007.2A CN201710796007A CN107365700A CN 107365700 A CN107365700 A CN 107365700A CN 201710796007 A CN201710796007 A CN 201710796007A CN 107365700 A CN107365700 A CN 107365700A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses a kind of placenta cells thawing apparatus and its method for resuscitation, water-bath that may indicate that recovery terminal, the recovery cell device composition being arranged in water-bath；Described recovery cell device is provided with recovery tank, clear water tanks and control panel, and control panel is provided with power switch, filtering controlling switch, temperature indicator, temperature control device and warning device, filtering controlling switch control pump working；The top of recovery tank is provided with recovery fixed plate, and the surface of recovery fixed plate is provided with fixing hole, and the hole shape of fixing hole and aperture are corresponding to cryopreservation tube, the form and dimension for freezing bag respectively；The lower surface of recovery fixed plate is provided with resilient clamp piece in the position of corresponding fixing hole.The present apparatus and method, bag or cryopreservation tube are frozen without man-hour manually hand-held in resuscitation process, realizes automation；The defects of whether recovering completion need to manually be visually inspected by overcoming, while overcome the problem of water capacity in water-bath easily brings pollution；The uniformity of recovery temperature is improved, improves the activity of cell.
Description
Technical field
The present invention relates to be exactly a kind of placenta cells thawing apparatus that may indicate that recovery terminal and its recovery side
Method.
Background technology
Patent CN204151347 discloses a kind of cell resuscitator of water-bath circulation, and the invention is by cryopreservation tube putting hole
Card hole is set to, cryopreservation tube is fixed in water-bath, and plays the effect with water segregation in resuscitator.But still have the disadvantage that
：1st, the terminal that cell recovery is completed needs artificial judgment；Although the 2, cell cryopreservation tube does not contact directly with water, introducing pollution is avoided
Risk, but the specific heat of air is much smaller than water, can slow down cryopreservation tube programming rate significantly, does not meet cell recovery " instant "
Technical requirements, cell additional injuries may be caused to reduce motility rate.
Patent CN201510694871 is disclosed a kind of to be implemented in combination with automatically by elevating mechanism and controller
High-volume cell recovery process, and time and the temperature of recovery can be accurately controlled, the procedure parameter of recovery is recorded, and avoid
Water in water-bath greatly improved cell recovery quality, alleviate the negative of operating personnel to freeze-stored cell possible pollution
Load, improves operating efficiency；But still have the disadvantage that：
1st, when its resuscitation process is controlled recovery by presetting the form of time combination measurement cell recovery environment temperature
Between and temperature, but its setting time cell not necessarily recover completely, its measurement temperature differ pole with cell actual temperature
Greatly.Therefore it still needs range estimation and judges whether cell recovery is completed, when otherwise cell temperature rises to more than 4 DEG C, in frozen stock solution
DMSO toxicity can rise significantly with temperature, so as to damaging cells.
Although the 2, cell cryopreservation tube does not contact directly with water, the risk for introducing pollution is avoided, the specific heat of air is remote
Less than water, cryopreservation tube programming rate can be slowed down significantly, the technical requirements of cell recovery " instant " are not met, cell volume may be caused
It is outer to damage so as to reduce motility rate.
The invention of above-mentioned cell recovery device, it is the device of recovery cell cryopreservation tube, but umbilical hemopoietic stem cell etc.
Field, cell, which is stored in, to be frozen in bag, and above-mentioned patent is not suitable for the recovery of cell cryopreservation bag.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of placenta cells thawing apparatus that may indicate that recovery terminal and its
Method for resuscitation, you can suitable for the recovery of cryopreservation tube, it is readily applicable to freeze the recovery of bag, and in resuscitation process, without
Man-hour manually hand-held freezes bag or cryopreservation tube, realizes automation；The defects of whether recovering completion need to manually be visually inspected by overcoming, together
When the water capacity that overcomes in water-bath the problem of easily bringing pollution；The uniformity of recovery temperature is improved, improves the activity of cell.
In order to solve the above technical problems, the present invention uses following technological means：
A kind of placenta cells thawing apparatus that may indicate that recovery terminal, including water-bath, the recovery cell being arranged in water-bath
Device, described recovery cell device are provided with recovery tank, clear water tanks and control panel, and the tank wall of recovery tank is provided with water inlet,
Water inlet is connected by water inlet pipe with water purification filter case, and the outlet pipe of water purification filter case is provided with water pump；In water purification filter case
Water is transported to recovery tank by water pump, and the side wall of recovery tank is provided with delivery port；Control panel is provided with power switch, filtering control is opened
Pass, temperature indicator, temperature control device and warning device, filtering controlling switch control pump working；The top of recovery tank is provided with multiple
Revive fixed plate, the surface of recovery fixed plate is provided with fixing hole, the hole shape of fixing hole and aperture respectively with cryopreservation tube, freeze the shape of bag
Shape and size are corresponding；The lower surface of recovery fixed plate is provided with resilient clamp piece, resilient clamp piece court in the position of corresponding fixing hole
To the side fixed temperature probe of fixing hole, on the resilient clamp piece of fixing hole corresponding with freezing bag temp probe point it is upper, in,
It is lower to set three；Temp probe is connected with the temperature control device in control panel, temperature control device connection control temperature indicator, alarm
Device.
Recovery fixed plate, the panel are detachable panel, by changing this panel, realize can recovery different size jelly
Pipe is deposited, can also recover freezes the function of bag；It is provided with fixing hole on panel, flexible holding piece in fixing hole, resilient clamp piece
The cryopreservation tube for cryopreservation tube can be fixed, freezing bag and can meet different-diameter uses.
The device and method of the technical program, for those skilled in the art, can be with beyond all doubt determination, i.e.,
Can be used for placenta cells, can be used for other cells, it is every by cryopreservation device as frozen bag or cryopreservation tube preserves,
It is applicable.
Resilient clamp piece, which also acts as, to be closely contacted to cryopreservation tube by surface temperature probe, freezes the effect of bag.
Described resilient clamp piece is provided with arc convex to inner side, and open-work, through-hole position fixed temperature are set at arc convex
Probe.
Above-mentioned setting, temp probe can be made to push against cryopreservation tube or freeze the surface of bag, thermometric is accurate.
Described recovery cell device is provided with casing, and one end of casing is provided with dividing plate, and casing is separated into recovery tank by dividing plate,
Clear water tanks；The top of clear water tanks sets control panel.
By setting dividing plate, casing is divided into two parts.
Described dividing plate is provided with the permeable hole being uniformly arranged, and the water purification of water pump output is transported to recovery by permeable hole
Case.
By setting permeable hole, make into recovery tank water evenly, thaw evenly.
The placenta cells thawing apparatus defreezing method of recovery terminal is may indicate that, is comprised the steps of：
（1）Temperature range is set：Set four temperature ranges to be monitored display to thawing by temperature control device, be respectively：-5
Low-temperature range below DEG C；- 5 DEG C -- 0 DEG C close to recovery temperature range；0 DEG C -- 4 DEG C of cell recovery temperature range；4 DEG C with
Upper is high temperature section；
（2）Temp probe and temperature indicator are set：Surface temperature measurement instrument connects temperature indicator, the setting of temperature indicator
It is as follows, for cryopreservation tube, at least provided with 2 temperature indicators, one temp probe is being set in face of the direction of current, carried
The direction of current sets a temp probe, the corresponding temp probe of each temperature indicator；For freezing bag, fixing hole
Both ends be respectively facing permeable hole, delivery port, symmetrically divide in two sides of bag upper, middle and lower set three temp probes, three temperature
Degree indicator lamp corresponds to the upper, middle and lower temp probe for freezing bag respectively；
（3）Temperature indicator color is set：For different temperature ranges, shown with different colors；
（4）Alarm setting：When the surface temperature of temp probe monitoring is more than 4 DEG C；Warning device sends alarm.
Using the present invention of above-mentioned technical proposal, compared with prior art, the characteristics of it is protruded, is：
（1）Temp probe is in close contact with testee, and the temperature of measurement is the surface temperature of testee, and monitoring is more accurate；
（2）The recovery applied widely for being applicable to cryopreservation tube, is readily applicable to freeze the recovery of bag；
（3）Recovery efficiency high, in resuscitation process, bag or cryopreservation tube are frozen without man-hour manually hand-held, realizes automation；
（4）The cytoactive of recovery is good, and the defects of whether recovering completion need to be manually visually inspected due to overcoming, is overcome simultaneously
The problem of water capacity in water-bath easily brings pollution, the uniformity of recovery temperature is improved, greatly improve the activity of cell.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram of the present invention.
Fig. 2 is a kind of structural representation of embodiment of recovery fixed plate of the present invention.
Fig. 3 is the structural representation of second of embodiment of recovery fixed plate of the present invention.
Fig. 4 is structural representation of the recovery tank with water purification filter case of the present invention.
Fig. 5 is a kind of top view of embodiment of fixing hole of the present invention.
Fig. 6 is the top view of second of embodiment of fixing hole of the present invention.
Fig. 7 be resilient clamp piece of the present invention, temp probe a kind of embodiment structural representation.
Description of reference numerals：1- water-baths；2- recovery cell devices；3- water inlets；4- power switches；5- temperature
Indicator lamp；6- control panels；7- temperature control devices；8- filters controlling switch；9- warning devices；10- recovery tanks；11- is answered
Soviet Union's fixed plate；12- fixing holes；13- water purification filter casees；14- water pumps；15- dividing plates；16- permeable holes；17- delivery ports；
18- temp probes；19- resilient clamp pieces；20- arc convex.
Embodiment
With reference to embodiment, the present invention is further illustrated.
Understood referring to Fig. 1-Fig. 3, a kind of placenta cells thawing apparatus that may indicate that recovery terminal of the invention, by water-bath
1, the recovery cell device 2 being arranged in water-bath 1 forms；Described recovery cell device 2 is provided with recovery tank 10, clear water tanks and
Control panel 6, the tank wall of recovery tank 10 are provided with water inlet 3, and water inlet 3 is connected by water inlet pipe with water purification filter case 13, only
The outlet pipe of water filtration case 13 is provided with water pump 14；Water in water purification filter case 13 is transported to recovery tank 10 by water pump 14, multiple
The side wall of Soviet Union's case 10 is provided with delivery port 17；Control panel 6 is provided with power switch 4, filtering controlling switch 8, temperature indicator 5, temperature
Device 7 and warning device 9 are controlled, filtering controlling switch 8 controls water pump 14 to work；The top of recovery tank 10 is provided with recovery fixed plate
11, the surface of recovery fixed plate 11 is provided with fixing hole 12, the hole shape of fixing hole 12 and aperture respectively with cryopreservation tube, freeze the shape of bag
Shape and size are corresponding；The lower surface of recovery fixed plate 11 is provided with resilient clamp piece 19, elastic clip in the position of corresponding fixing hole 12
Side fixed temperature probe 18 of the piece 19 towards fixing hole 12 is held, on the resilient clamp piece 19 of fixing hole 12 corresponding with freezing bag
Temp probe 18 divides upper, middle and lower to set three；Temp probe 18 is connected with the temperature control device 7 in control panel 6, temperature control device 7
Connection control temperature indicator 5, warning device 9.
Recovery fixed plate 11, the panel are detachable panel, and by changing this panel, realizing can recovery different size
Cryopreservation tube, it can also recover and freeze the function of bag；It is provided with fixing hole 12 on panel, flexible holding piece 19 in fixing hole 12, bullet
Property holding piece 19 can fix cryopreservation tube, the cryopreservation tube that freezes bag and can meet different-diameter uses.
Resilient clamp piece 19, which also acts as, to be closely contacted to cryopreservation tube by surface temperature probe 18, freezes the effect of bag.
Described resilient clamp piece 19 is provided with arc convex 20 to inner side, sets open-work at arc convex 20, and through-hole position is solid
Determine temp probe 18.
Above-mentioned setting, temp probe 18 can be made to push against cryopreservation tube or freeze the surface of bag, thermometric is accurate.
Described recovery cell device 2 is provided with casing, and one end of casing is provided with dividing plate 15, and casing is separated into multiple by dividing plate 15
Soviet Union's case 10, clear water tanks；The top of clear water tanks sets control panel 6.
By setting dividing plate 15, casing is divided into two parts.
Described dividing plate 15 is provided with the permeable hole 16 being uniformly arranged, and the water purification that water pump 14 exports is conveyed by permeable hole 16
To recovery tank 10.
By setting permeable hole 16, make into recovery tank 10 water evenly, thaw evenly.
The placenta cells thawing apparatus defreezing method of recovery terminal is may indicate that, is comprised the steps of：
（1）Temperature range is set：Set four temperature ranges to be monitored display to thawing by temperature control device 7, be respectively：-5
Low-temperature range below DEG C；- 5 DEG C -- 0 DEG C close to recovery temperature range；0 DEG C -- 4 DEG C of cell recovery temperature range；4 DEG C with
Upper is high temperature section；
（2）Temp probe 18 and temperature indicator 5 are set：Surface temperature measurement instrument connects temperature indicator 5, temperature indicator 5
Setting it is as follows, for cryopreservation tube, at least provided with 2 temperature indicators 5, one temp probe is being set in face of the direction of current
18, a temp probe 18, the corresponding temp probe 18 of each temperature indicator 5 are set in the direction for carrying current；It is right
In freezing bag, the both ends of fixing hole 12 are respectively facing permeable hole 16, delivery port 17, and in two sides of bag, symmetrically a point upper, middle and lower is set
Three temp probes 18 are put, three temperature indicators 5 correspond to the upper, middle and lower temp probe 18 for freezing bag respectively；
（3）The color of temperature indicator 5 is set：For different temperature ranges, shown with different colors；
（4）Alarm setting：When the surface temperature that temp probe 18 monitors is more than 4 DEG C；Warning device 9 sends alarm.
Recovery contrast experiment one：Description of test：In same environment, 100 cryopreservation tubes are respectively taken；Using traditional approach and originally
Device and method solves to freeze.
Recovery contrast experiment two：Description of test：In same environment, 100 are respectively taken to freeze bag；Using traditional approach and originally
Device and method solves to freeze.
Experiment conclusion：The present apparatus and method, first, recovery efficiency can be improved, second, the consistent of recovery temperature can be improved
Property, the activity of average cell is improved, reduces cell contamination rate in resuscitation process.
Using the present invention of above-mentioned technical proposal, compared with prior art, the characteristics of it is protruded, is：
（1）Temp probe 18 is in close contact with testee, and the temperature of measurement is the surface temperature of testee, and monitoring is more accurate
Really；
（2）The recovery applied widely for being applicable to cryopreservation tube, is readily applicable to freeze the recovery of bag；
（3）Recovery efficiency high, in resuscitation process, bag or cryopreservation tube are frozen without man-hour manually hand-held, realizes automation；
（4）The cytoactive of recovery is good, and the defects of whether recovering completion need to be manually visually inspected due to overcoming, is overcome simultaneously
The problem of water capacity in water-bath easily brings pollution, the uniformity of recovery temperature is improved, greatly improve the activity of cell.
Due to the foregoing is only the embodiment of the present invention, but the protection not limited to this of the present invention, any skill
The technical staff in art field it is contemplated that the equivalent change of the technical program technical characteristic or replacement, all cover the present invention's
Within protection domain.
Claims (5)
1. a kind of placenta cells thawing apparatus that may indicate that recovery terminal, including water-bath, the recovery being arranged in water-bath are thin
Born of the same parents' device, it is characterised in that：Described recovery cell device is provided with recovery tank, clear water tanks and control panel, the tank wall of recovery tank
Water inlet is provided with, water inlet is connected by water inlet pipe with water purification filter case, and the outlet pipe of water purification filter case is provided with water pump；Only
Water in water filtration case is transported to recovery tank by water pump, and the side wall of recovery tank is provided with delivery port；Control panel is opened provided with power supply
Close, filtering controlling switch, temperature indicator, temperature control device and warning device, filtering controlling switch control pump working；Recovery tank
Top be provided with recovery fixed plate, the surface of recovery fixed plate is provided with fixing hole, and the hole shape of fixing hole and aperture are respectively with freezing
The form and dimension for managing, freezing bag is corresponding；The lower surface of recovery fixed plate is provided with resilient clamp piece in the position of corresponding fixing hole,
The side fixed temperature of resilient clamp piece towards fixing hole is popped one's head in, temperature on the resilient clamp piece of fixing hole corresponding with freezing bag
Probe divides upper, middle and lower to set three；Temp probe is connected with the temperature control device in control panel, temperature control device connection control temperature
Spend indicator lamp, warning device.
2. the placenta cells thawing apparatus according to claim 1 that may indicate that recovery terminal, it is characterised in that：Described bullet
Property holding piece be provided with arc convex to inner side, open-work, through-hole position fixed temperature probe are set at arc convex.
3. the placenta cells thawing apparatus according to claim 1 that may indicate that recovery terminal, it is characterised in that：Described answers
Soviet Union's cell device is provided with casing, and one end of casing is provided with dividing plate, and casing is separated into recovery tank, clear water tanks by dividing plate；Clear water tanks
Top sets control panel.
4. the placenta cells thawing apparatus according to claim 3 that may indicate that recovery terminal, it is characterised in that：It is described every
Plate is provided with the permeable hole being uniformly arranged, and the water purification of water pump output is transported to recovery tank by permeable hole.
5. the placenta cells thawing apparatus defreezing method according to claim 1 that may indicate that recovery terminal, it is characterised in that
Comprise the steps of：(1) temperature range is set：Four temperature ranges are set to be monitored display to thawing by temperature control device,
Respectively：Less than -5 DEG C of low-temperature range；- 5 DEG C -- 0 DEG C close to recovery temperature range；0 DEG C -- 4 DEG C of cell recovery temperature
Section；More than 4 DEG C are high temperature section；（2）Temp probe and temperature indicator are set：The connection temperature instruction of surface temperature measurement instrument
Lamp, the setting of temperature indicator is as follows, for cryopreservation tube, at least provided with 2 temperature indicators, is set in face of the direction of current
One temp probe, a temp probe is set in the direction for carrying current, and the corresponding temperature of each temperature indicator is visited
Head；For freezing bag, the both ends of fixing hole are respectively facing permeable hole, delivery port, and in two sides of bag, symmetrically a point upper, middle and lower is set
Three temp probes are put, three temperature indicators correspond to the upper, middle and lower temp probe for freezing bag respectively；（3）Temperature indicator color
Set：For different temperature ranges, shown with different colors；（4）Alarm setting：The surface temperature of temp probe monitoring is big
When 4 DEG C；Warning device sends alarm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710796007.2A CN107365700A (en) | 2017-09-06 | 2017-09-06 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710796007.2A CN107365700A (en) | 2017-09-06 | 2017-09-06 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
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| Publication Number | Publication Date |
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| CN107365700A true CN107365700A (en) | 2017-11-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710796007.2A Pending CN107365700A (en) | 2017-09-06 | 2017-09-06 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
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| CN204768805U (en) * | 2015-07-03 | 2015-11-18 | 济南赛尔生物科技有限公司 | Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell |
| CN204816593U (en) * | 2015-06-24 | 2015-12-02 | 佛山迪安医学检验所有限公司 | Water bath with dual temperature monitoring |
| CN205473725U (en) * | 2016-04-08 | 2016-08-17 | 西安医学院 | Cell recovery box is deposited with portable freezing in laboratory |
| CN206157132U (en) * | 2016-08-24 | 2017-05-10 | 天津市康婷生物工程有限公司 | Handy cell recovery device |
| CN106995782A (en) * | 2017-04-24 | 2017-08-01 | 何向锋 | A kind of dry type cell recovery instrument |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1220645A (en) * | 1985-01-18 | 1987-04-21 | Maritonex Inc. | Alarm system for monitoring the temperature of a liquid contained in a reservoir |
| CN204509348U (en) * | 2015-03-13 | 2015-07-29 | 通威股份有限公司 | A kind of use for laboratory enzymatic reaction device |
| CN204816593U (en) * | 2015-06-24 | 2015-12-02 | 佛山迪安医学检验所有限公司 | Water bath with dual temperature monitoring |
| CN204768805U (en) * | 2015-07-03 | 2015-11-18 | 济南赛尔生物科技有限公司 | Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell |
| CN205473725U (en) * | 2016-04-08 | 2016-08-17 | 西安医学院 | Cell recovery box is deposited with portable freezing in laboratory |
| CN206157132U (en) * | 2016-08-24 | 2017-05-10 | 天津市康婷生物工程有限公司 | Handy cell recovery device |
| CN106995782A (en) * | 2017-04-24 | 2017-08-01 | 何向锋 | A kind of dry type cell recovery instrument |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| CN109749929B (en) * | 2019-02-22 | 2022-08-19 | 中国人民解放军第四军医大学 | Device is hatched to separation brain piece rewarming of real-time supervision state |
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