CN204768805U - Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell - Google Patents
Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell Download PDFInfo
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- CN204768805U CN204768805U CN201520468778.5U CN201520468778U CN204768805U CN 204768805 U CN204768805 U CN 204768805U CN 201520468778 U CN201520468778 U CN 201520468778U CN 204768805 U CN204768805 U CN 204768805U
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Abstract
The utility model provides a be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell, by the water -bath box, take control panel the control box, constitute with the non - shock board be connected of coaxial hinge nature and coaxial rigid connection's agitating unit with transmission, wherein agitating unit is by the motor, vertical bent axle in the control box, vertical bent axle in the water -bath box, camshaft connecting rod, stirring fan and two horizontal dead levers that are fixed in water -bath box inner wall constitute, motor and the interior vertical the crankshaft of control box, this bent axle is provided with the stirring fan rather than coaxial rigid connection through camshaft connecting rod with the lower part again, upper portion is provided with rather than vertical the crankshaft in the water -bath box of the shock board of non - coaxial hinge nature connection, it is fixed with the interior wall connection of water -bath box that shock board four corners is provided with the spring respectively. This device can shorten to freeze to be deposited the cell used time of recovery to the messenger is frozen and is deposited the mouth of pipe and not in the water intrusion, reduced the probability that the recovery cell pollutes, and many can once only be handled to this device freezes and deposits the cell, has improved recovery efficiency.
Description
Technical field
The utility model relates to a kind of water-bath, particularly relates to a kind of even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell.
Background technology
A kind of technological means that freeze-stored cell recovery is biology and medically commonly uses, its principle is rapid fluid resuscitation in 37 DEG C of water-baths, and during to reduce recovery cell, DMSO is to the infringement of cell.Traditional recovery cell way cryopreservation tube is directly put into 37 DEG C of water-baths, and constantly rock cryopreservation tube with hand, makes its fast melt.But the mouth of pipe can be made unavoidably to be immersed in water-bath because hand-held cryopreservation tube rocks in the process, cause the probability of cell contamination in resuscitation process to increase.Meanwhile, because constantly rocking with hand-held cryopreservation tube, the contact probability of hand and cell and time increase, and also increase the risk of cell contamination in subsequent operation; And traditional method for resuscitation is due to the Limited Number of hand-held cryopreservation tube, also limit quantity and the operating efficiency of recovery cell.
The water-bath that current recovery freeze-stored cell uses due to heater following at water-bath, and water above contacts with air, the water temperature above water-bath can be caused lower than the water temperature below water-bath, the experiment condition inaccuracy of recovery cell can be made like this, thus affect cell recovery yield.And water-bath seldom has timer, when recovery cell, experimenter can only set separately the recovery cell time used or simply guess, causes cell recovery intricate operation or overlong time, causes cell recovery motility rate to reduce.
Utility model content
For the deficiencies in the prior art, problem to be solved in the utility model is a kind of even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell.
The even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell described in the utility model, the agitating device connected by the control cabinet of water-bath casing, band control panel, the concussion plate be connected with non-coaxial hinge property with transmission device and coaxial rigid is formed, it is characterized in that: the control cabinet of described band control panel is in the side of water-bath casing, control panel is made up of the LCDs of four buttons and switch key, I speed governing button, II speed governing button, timing button and an energy displaying time and rotating speed, wherein said switch key is connected with battery, I speed governing button or II speed governing button and motors, timing button is connected with LCDs clock, and motor is connected with battery respectively with clock, control cabinet is positioned at below control panel, box sealing waterproof and with water-bath casing with high, be provided with battery and motor in its casing, described agitating device is by motor, North-south crankshaft in control cabinet, North-south crankshaft in water-bath casing, crankshaft-link rod, agitation fan and two horizontal fixed bars being fixed on water-bath cabinet wall are formed, wherein motor is connected with North-south crankshaft in control cabinet, this bent axle is connected with North-south crankshaft in water-bath casing by crankshaft-link rod again, in described water-bath casing, North-south crankshaft bottom is provided with the agitation fan be connected with its coaxial rigid, top is provided with the concussion plate be connected with its non-coaxial hinge property, concussion plate corner is respectively arranged with spring and water-bath cabinet wall is connected and fixed.
Above-mentioned is suitable in the even temperature timing water-bath of rapid fluid resuscitation freeze-stored cell: the corner of described water-bath casing is preferably fixed with a soft iron sheet, each soft iron sheet top is fixed with a permanent magnet, magnet attachment is used at water-bath inwall the concussion plate that fixing corner is provided with spring, along with soft iron bending tablet can corresponding adjustment magnet attachment position, and then realize the adjustment concussion plate effect fair with water-bath liquid level.
Above-mentioned is suitable in the even temperature timing water-bath of rapid fluid resuscitation freeze-stored cell: described concussion plate is evenly distributed with the hole of placing cryopreservation tube, and the diameter of its diameter and cryopreservation tube matches.
The utility model provides a kind of safety and the even temperature timing water-bath of rapid fluid resuscitation freeze-stored cell, and this device can shorten the freeze-stored cell recovery time used, improves the recovery motility rate of freeze-stored cell; Simultaneously because the frozen mouth of pipe is not immersed in the water, reduce the probability of recovery cell contamination; And this device can the branched freeze-stored cell of disposable process, improves recovery efficiency.
The principle that the even temperature of the utility model rocks cryopreservation tube is: by the rotation controlling motor, North-south crankshaft in control cabinet is rotated, this bent axle drives North-south crankshaft in water-bath casing to rotate by crankshaft-link rod again, in described water-bath casing, North-south crankshaft bottom is provided with the agitation fan be connected with its coaxial rigid, top is provided with the concussion plate be connected with its non-coaxial hinge property, and the rotation of bent axle can drive the rotation of agitation fan and concussion plate to do circumference concussion.The equilibrium and concussion plate and the water-bath liquid level that achieve water temperature are at a stalemate flat, make the process of cryopreservation tube constant temperature and the mouth of pipe is not immersed in water-bath, reduce the probability of pollution, improve cell recovery motility rate.
Device of the present utility model compares with existing product, advantageously:
1, this device adds concussion plate, shortens recovery cell stage, improves recovery Cell viability and anabiosis rate.And disposablely can process the recovery of branched freeze-stored cell, improve recovery cell efficiency.
2, the free display screen of control cabinet, makes experimenter have the cell recovery time and experiences intuitively.
3, control cabinet can control the speed of motor by speed governing button, thus controls the rotating speed of agitation fan and the concussion speed of concussion frame, to adapt to different cell recovery.
4, this device utilizes agitation fan, makes the water above water-bath consistent with water temperature below, precision test condition.
5, this device utilizes magnet to be fixed on water-bath inwall, and can adjust attachment position, and adjustment concussion plate and water-bath liquid level flat position at a stalemate, make the cryopreservation tube mouth of pipe not be immersed in water-bath, reduces the probability polluted.
6, this device utilizes soft iron sheet to adjust stretching degree, to adapt to the water-bath of different size.
7, experimenter has departed from hand operated, uses this device more convenient.
Accompanying drawing explanation
Fig. 1: the three-dimensional structure diagram being suitable for the even temperature timing water-bath of rapid fluid resuscitation freeze-stored cell described in the utility model.
Wherein: 1-spring, 2-shakes frame, 3-battery, 4-motor, 5-control cabinet, and 6, North-south crankshaft in control cabinet, 7-casing, 8-soft iron sheet, 9-magnet, 10-fixed bar, 11-agitation fan, 12-crankshaft-link rod, North-south crankshaft in 13-water-bath casing.
Fig. 2: the even temperature timing water-bath assembly connecting mode schematic diagram being suitable for rapid fluid resuscitation freeze-stored cell described in the utility model.
In figure
represent hinged connected mode;
represent the connected mode just connect; Other same Fig. 1.
Detailed description of the invention
Embodiment 1:
The novel core of this practicality is to provide a kind of even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell, utilizes this device can rapid fluid resuscitation freeze-stored cell effectively, thus the motility rate improving recovery cell with improve recovery efficiency.
Below by embodiment, the utility model will be further described by reference to the accompanying drawings:
As shown in Figure 1 and Figure 2, the present embodiment describes the even temperature regularly water-bath being suitable for rapid fluid resuscitation freeze-stored cell, and the agitation fan 11 connected by the control cabinet 5 of water-bath casing 7, band control panel, the concussion plate 2 cutting with scissors property connection with non-coaxial and coaxial rigid is formed.The control cabinet of wherein said band control panel is in the side of water-bath casing 7, control panel is made up of the LCDs of four buttons and switch key, I speed governing button, II speed governing button, timing button and an energy displaying time and rotating speed, wherein said switch key is connected with battery 3, I speed governing button or II speed governing button are connected with motor 4, timing button is connected with LCDs clock, and motor is connected with battery respectively with clock, control cabinet 5 is positioned at below control panel, box sealing waterproof and with water-bath casing with high, be provided with battery and motor in its casing, described agitating device is by motor, North-south crankshaft 6 in control cabinet, North-south crankshaft 13 in water-bath casing, crankshaft-link rod 12, agitation fan 11 and two horizontal fixed bars 10 being fixed on water-bath cabinet wall are formed, wherein motor is connected with North-south crankshaft in control cabinet, this bent axle is connected with North-south crankshaft in water-bath casing by crankshaft-link rod again, in described water-bath casing, North-south crankshaft bottom is provided with the agitation fan be connected with its coaxial rigid, top is provided with the concussion plate 2 be connected with its non-coaxial hinge property, concussion plate corner is respectively arranged with spring 1 and is connected and fixed with water-bath cabinet wall.
The Corner Strapped of above-mentioned water-bath casing has a soft iron sheet 8, each soft iron sheet top is fixed with a permanent magnet 9, magnet attachment is used at water-bath inwall the concussion plate 2 that fixing corner is provided with spring, along with soft iron bending tablet can corresponding adjustment magnet attachment position, and then realize the adjustment concussion plate effect fair with water-bath liquid level.
Above-mentioned concussion plate 2 is evenly distributed with the hole of placing cryopreservation tube, and the diameter of its diameter and cryopreservation tube matches.
Concrete, the battery of described motor uses rechargeable battery, and control cabinet, motor, battery need to do water-proofing treatment.Each angle of casing 7 is fixed with a soft iron sheet 8, and each soft iron sheet 8 top is fixed with a permanent magnet 9.Soft iron sheet 8 can adjust stretching degree, to adapt to the water-bath of different size, this device can be attached to water-bath inwall by magnet, play fixation, and can by adjustment attachment position, and then play adjustment concussion frame and water-bath liquid level flat position at a stalemate, the cryopreservation tube mouth of pipe is not immersed in water-bath, reduces the probability polluted.
Control panel on control cabinet 5 has three buttons and a LCDs, button is switch, I speed governing button, II speed governing button respectively, switch control rule power supply, the rotating speed of key control motor is pressed in I speed governing button and II speed governing, and then control the concussion frequency of concussion frame, to adapt to the requirement of different cell recoveries.LCDs display recovery starts the rear time used, allows experimenter have the recovery cell time used and experiences intuitively.
Power set motor 4 directly and in control cabinet North-south crankshaft 6 be rigidly connected, in control cabinet, North-south crankshaft 6 is connected with North-south crankshaft 13 in water-bath casing by crankshaft-link rod 12, in water-bath casing, North-south crankshaft 13 bottom is connected with agitation fan 11 coaxial rigid, in water-bath casing, North-south crankshaft 13 top is connected with concussion frame 2 non-coaxial hinge property, because North-south crankshaft 13 in water-bath casing is connected with concussion frame 2 non-coaxial, there is wheelbase between the two, so when in water-bath casing, North-south crankshaft 13 rotates, concussion frame 12 also can along with doing circumference vibrations.Bent axle 6,13 is fixed by being fixed on casing 7 inwall two fixed bars 10, and the connected mode of bent axle 6,13 and fixed bar 10 is hinged connected mode.
When using the utility model, regulate the stretching degree of soft iron sheet 8, make it and water-bath match specifications.This device box 7 is anchored at water-bath inwall by magnet 9, and adjust set position to concussion plate 2 and water-bath water-bath liquid level flat position at a stalemate, cryopreservation tube is put into the hole of concussion plate 2, press the shift knob of control cabinet 5, characteristic according to cell selects rotating speed I speed governing button or II speed governing button, after pressing speed governing button, LCDs on control cabinet 5 will self-clocking, the showed cell recovery time used, concussion plate 2 can be driven to shake for motor 4 rotation simultaneously and agitation fan 11 stirs, after arriving the scheduled time, freeze-stored cell is taken out from concussion plate 7, carry out next step test.
Claims (3)
1. be suitable for an even temperature timing water-bath for rapid fluid resuscitation freeze-stored cell, the agitating device connected by the control cabinet of water-bath casing, band control panel, the concussion plate be connected with non-coaxial hinge property with transmission device and coaxial rigid is formed, it is characterized in that: the control cabinet of described band control panel is in the side of water-bath casing, control panel is made up of the LCDs of four buttons and switch key, I speed governing button, II speed governing button, timing button and an energy displaying time and rotating speed, wherein said switch key is connected with battery, I speed governing button or II speed governing button and motors, timing button is connected with LCDs clock, and motor is connected with battery respectively with clock, control cabinet is positioned at below control panel, box sealing waterproof and with water-bath casing with high, be provided with battery and motor in its casing, described agitating device is by motor, North-south crankshaft in control cabinet, North-south crankshaft in water-bath casing, crankshaft-link rod, agitation fan and two horizontal fixed bars being fixed on water-bath cabinet wall are formed, wherein motor is connected with North-south crankshaft in control cabinet, this bent axle is connected with North-south crankshaft in water-bath casing by crankshaft-link rod again, in described water-bath casing, North-south crankshaft bottom is provided with the agitation fan be connected with its coaxial rigid, top is provided with the concussion plate be connected with its non-coaxial hinge property, concussion plate corner is respectively arranged with spring and water-bath cabinet wall is connected and fixed.
2. the even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell according to claim 1, it is characterized in that: the Corner Strapped of described water-bath casing has a soft iron sheet, each soft iron sheet top is fixed with a permanent magnet, magnet attachment is used at water-bath inwall the concussion plate that fixing corner is provided with spring, along with soft iron bending tablet can corresponding adjustment magnet attachment position, and then realize the adjustment concussion plate effect fair with water-bath liquid level.
3. the even temperature timing water-bath being suitable for rapid fluid resuscitation freeze-stored cell according to claim 1, is characterized in that: described concussion plate is evenly distributed with the hole of placing cryopreservation tube, and the diameter of its diameter and cryopreservation tube matches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520468778.5U CN204768805U (en) | 2015-07-03 | 2015-07-03 | Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520468778.5U CN204768805U (en) | 2015-07-03 | 2015-07-03 | Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell |
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| CN204768805U true CN204768805U (en) | 2015-11-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520468778.5U Active CN204768805U (en) | 2015-07-03 | 2015-07-03 | Be suitable for quick recovery to freeze even temperature water -bath regularly of depositing cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365700A (en) * | 2017-09-06 | 2017-11-21 | 银丰生物工程集团有限公司 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
| CN109647560A (en) * | 2019-01-23 | 2019-04-19 | 南方医科大学南方医院 | A kind of cryopreservation tube reconstitution device and its implementation |
-
2015
- 2015-07-03 CN CN201520468778.5U patent/CN204768805U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365700A (en) * | 2017-09-06 | 2017-11-21 | 银丰生物工程集团有限公司 | A kind of placenta cells thawing apparatus and its method for resuscitation that may indicate that recovery terminal |
| CN109647560A (en) * | 2019-01-23 | 2019-04-19 | 南方医科大学南方医院 | A kind of cryopreservation tube reconstitution device and its implementation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 250101 Shandong Jinan Hi-tech Zone (Comprehensive Protection Zone) 13 Floors, Block B, Building No. 1, Yaogu, Jinan, North Section of Gangxing No. 3 Road Patentee after: Ji'nan biological Polytron Technologies Inc Address before: Room B-201, Comprehensive Building of University Science Park, 750 Shunhua Road, Jinan High-tech Zone, Shandong Province, 250100 Patentee before: Jinan Cell Bio-Technology Co., Ltd. |
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| CP03 | Change of name, title or address |