CN212713578U

CN212713578U - Batch cell resuscitator

Info

Publication number: CN212713578U
Application number: CN202021377817.8U
Authority: CN
Inventors: 李志远; 汤沙; 严波
Original assignee: Hunan Huiyisen Cell Genetic Engineering Co ltd
Current assignee: Hunan Huiyisen Cell Genetic Engineering Co ltd
Priority date: 2020-07-14
Filing date: 2020-07-14
Publication date: 2021-03-16
Anticipated expiration: 2030-07-14

Abstract

The utility model discloses a batch cell resuscitator, including bowl cover, cup, cryopreserved pipe tray can be along with the swing of triangle axis of rotation for cell resuscitation makes the cell be heated evenly, prevents to form the ice crystal at the process of thawing. The cup seat is provided with a temperature adjusting button and a speed control button for accurately controlling the temperature and the speed, and the cup body and the freezing tube tray are made of transparent materials and can observe the cell recovery situation at any time. The cell resuscitator can be operated by one person, shortens the resuscitating time, ensures the cell survival rate, improves the efficiency and reduces the workload.

Description

Batch cell resuscitator

Technical Field

The utility model relates to a cell recovery experimental facilities field specifically is a batch cell recovery appearance.

Background

Cell recovery is a process reverse to cell cryopreservation, and is a process of thawing cells frozen in a liquid nitrogen tank below-196 ℃ and then allowing the cells to recover growth. Different from the cell freezing, the temperature rise in the cell recovery process is fast, so that the water is prevented from entering the cells in the unfreezing process to form ice crystals, and the cell recovery survival rate is prevented from being influenced.

When the cells are recovered, the best operation is to rapidly heat the cell freezing tube, and the cells are most easily damaged at-5-0 ℃ rapidly, so as to avoid the damage of the ice crystal recrystallization to the cells and the cell death. At the same time, the cell freezing tube is shaken in the water bath from time to heat the cell freezing tube uniformly. In actual operation, cell thawing is mostly achieved by manually clamping a cell freezing tube and placing the tube in a water bath kettle for manual water bath thawing, and the traditional method has the defects that the number of cells thawed at the same time is too small, the water temperature is not constant, shaking force is not uniform, the cells are heated unevenly, and the like.

The cell thawing condition of the resuscitator can not be observed at any time by the cell resuscitator provided by the prior or other invention patents and utility models, thereby shortening the cell resuscitating time and improving the cell survival rate. The cell freezing tubes at multiple ultralow temperatures cause the local water temperature of the cell resuscitator to drop, the water in the resuscitator cannot be stirred effectively and quickly to enable the water temperature to be integrally constant, the rapid heating effect is realized, the resuscitating time is prolonged, the repeated crystallization risk is increased, and the cell activity and the survival rate are influenced.

Therefore, it is urgently needed to provide a batch cell resuscitator which is uniformly heated and is convenient to observe and operate.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a batch cell recovery appearance solves the problem that the above-mentioned exists, realizes cell recovery steadily high-efficiently, the utility model discloses a following technical scheme:

a batch cell resuscitator comprises a cup cover 1, a cup body 2, a cup seat 3 and a cryopreservation tube tray 4, wherein the cup cover 1 is buckled at the upper end of the cup body 2; the cup body 2 is a cylindrical structure with a hollow interior; the cup base 3 is fixedly connected with the cup body 2 at the lower end of the cup body 2 in a sealing way, the center of the cup base is provided with a triangular rotating shaft 5 protruding out of the cup body 2, a control unit, a heater 6 and a motor 7 are arranged in the cup base 3, a protruding temperature sensor 8 is arranged at the upper side of the cup base 3, and a liquid crystal display 9, a speed control button 10, a temperature adjusting button 11 and a power switch 12 are arranged at the outer side of the cup base 3; the freezing pipe tray 4 be located inside cup 2, constitute by two concentric cylinders, interior circle 17 center has to hold movable hole 13 and triangle axis of rotation 5 to be connected, excircle 18 is equipped with several inner wall and horizontal plane vertically fixed orifices 14, interior circle is connected through 8 stirring vane 19 that personally submit 45 degrees angles with the horizontal plane with the excircle.

Preferably, the joint of the triangular rotating shaft 5 and the cup base 3 is a sealed leakproof bearing 15, and the triangular rotating shaft 5 is driven by a motor 7 in the cup base 3.

Preferably, the fixing hole 14 has a sample holder 16 at the center thereof, and is opened to the vial tray 4 to allow water to enter the fixing hole 14.

Preferably, the rotation mode of the freezing tube tray 4 is that the freezing tube tray rotates clockwise half circle and then rotates anticlockwise half circle at once, and the freezing tube tray rotates circularly.

Preferably, the upper surface of the cup holder 3 is made of stainless steel.

Preferably, the material of the cup body 2 is transparent glass or transparent Polycarbonate (PC) or styrene acrylonitrile or styrene-methyl methacrylate copolymer.

Preferably, the vial tray 4 is made of transparent Polycarbonate (PC) or styrene acrylonitrile.

The utility model has the advantages that:

1. the utility model provides a batch cell resuscitator, which drives a freezing tube tray to vibrate through a motor, and simultaneously, stirring blades in the tray can quickly stir water in the instrument, so that the integral constant water temperature is realized, and the cell thawing speed is improved;

2. the cup body and the freezing tube tray of the cell resuscitator are made of transparent materials, and the thawing condition of each freezing cell in the freezing tube tray can be observed at any time by closing the switch, so that the resuscitating time is shortened, and the cell survival rate is improved;

3. the freezing tube tray can bear a plurality of cell freezing containers once, realizes automatic large-batch cell resuscitation, can accurately control resuscitation time and temperature, greatly improves cell resuscitation quality and quantity, reduces workload of workers, and improves work efficiency.

Drawings

Fig. 1 is a schematic structural diagram of a batch cell resuscitator provided by the present invention.

Fig. 2 is a plan view of the freezing storage pipe tray of the present invention.

Labeled as: 1. a cup cover; 2. a cup body; 3. a cup holder; 4. freezing and storing the tube tray; 5. a triangular rotating shaft; 6. a heater; 7. an electric motor; 8. a temperature sensor; 9. a liquid crystal display; 10. a speed control button; 11. a temperature adjustment button; 12. a power switch; 13. a bearing hole; 14. a fixing hole; 15. sealing the leak-proof bearing; 16. a sample holder; 17. an inner circle; 18. an outer circle; 19. the blades are agitated.

Detailed Description

For the purpose of clearly illustrating the objects, technical solutions and advantages of the present invention, the following brief description of the drawings, which are needed for practical purposes in the description of the embodiments or the prior art, will be provided. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.

A batch cell resuscitator comprises a cup cover, a cup body, a cup seat and a cryopreservation tube tray, wherein the cup cover is buckled at the upper end of the cup body; the cup body is a cylindrical structure with a hollow interior; the cup seat is arranged at the lower end of the cup body and is fixedly connected with the cup body in a sealing way, the center of the cup seat is provided with a triangular rotating shaft protruding towards the cup body, a control unit, a heater and a motor are arranged in the cup seat, a protruding temperature sensor is arranged at the upper side of the cup seat, and a liquid crystal display, a speed control button, a temperature adjusting button and a power switch are arranged at the outer side of the cup seat; the freezing pipe tray is located inside the cup and comprises two concentric cylinders, the center of the inner circle is provided with a bearing hole connected with a triangular rotating shaft, the outer circle is provided with a plurality of inner walls and a fixing hole vertical to the horizontal plane, and the inner circle and the outer circle are connected through 8 stirring blades which are arranged at an angle of 45 degrees with the horizontal plane. The rotation mode of the freezing tube tray is clockwise half circle and then anticlockwise half circle at once, and the freezing tube tray rotates circularly. When the resuscitator is started, the stirring blades stir water in the cup body, and convection is accelerated to enable the temperature in the resuscitator to be integrally constant.

The junction of triangle axis of rotation and cup seat is sealed leak protection bearing, prevents that the water in the cup from leaking into the cup seat, and the triangle axis of rotation is driven by the inside motor of cup seat. There is the sample support fixed orifices middle part, and the trompil makes inside water entering fixed orifices to the cryopreservation pipe tray. The upper side surface of the cup base is made of stainless steel materials, and the stainless steel materials are tightly connected with a heater in the cup base, so that the heat conduction rate is improved, and the constancy of the temperature in an instrument is facilitated. The cup body and the freezing tube tray are made of transparent materials, so that the thawing condition of the freezing cells can be directly observed, and the recovery time is shortened.

The working process of the cell resuscitator is as follows: during the use, pour into appropriate amount of water into batch cell resuscitator, set up the operating temperature in the cup through temperature regulation button, reach the settlement temperature, place the cell that will resuscitate in the fixed orifices of freezing pipe tray after preheating the completion, cover the bowl cover, select suitable speed through speed regulation button, begin to resuscitate, close the instrument at any time through switch, directly see through the cup, the freezing pipe tray observation cell condition of unfreezing, ice-cube in the freezing pipe just melts completely and can shut down, accomplish cell resuscitation, shorten the time of resuscitating, improve the cell survival rate.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the same, and although the present invention is described in detail with reference to the examples, it should be understood that the technical solutions of the present invention can be modified or replaced with other equivalent solutions without departing from the spirit and principle of the technical solutions of the present invention, which should be included in the protection scope of the present invention.

Claims

1. The utility model provides a batch cell resuscitation appearance, a serial communication port, including bowl cover, cup, cryopreserved pipe tray, the bowl cover lock joint is in the upper end of cup, the cup be the inside fretwork of cylindrical structure, the cup at the cup lower extreme, with the sealed fixed connection of cup, central authorities have the outstanding triangle axis of rotation of cup, there are heater, motor in the cup inside, there is outstanding temperature-sensing ware the cup upside, there are LCD, speed control button, temperature regulation button, switch in the cup outside, cryopreserved pipe tray be located inside the cup, constitute by two concentric cylinders, the interior circle center has bearing hole and triangle axis of rotation to be connected, the excircle is equipped with several inner wall and horizontal plane vertically fixed orifices, interior circle is connected through 8 stirring blades that personally submit 45 degrees angles with the horizontal plane with the excircle.

2. The apparatus according to claim 1, wherein the triangular rotating shaft is connected to the cup holder by a sealing and anti-leaking bearing, and the triangular rotating shaft is driven by a motor inside the cup holder.

3. The apparatus according to claim 1, wherein the fixing hole has a sample holder at the middle part, and a hole is formed in the freezing tube tray to allow water to enter the fixing hole.

4. The apparatus according to claim 1, wherein the tray of the vial rotates clockwise and then counterclockwise in a circular manner.

5. The apparatus according to claim 1, wherein the upper surface of the cup holder is made of stainless steel material and is tightly connected to the heater in the cup holder.

6. The apparatus according to claim 1, wherein the cup is made of transparent glass or transparent Polycarbonate (PC) or styrene acrylonitrile or styrene-methyl methacrylate copolymer.

7. The apparatus according to claim 1, wherein the vial holder is made of transparent Polycarbonate (PC) or styrene acrylonitrile.