CN106267344B - A kind of de- cell system of cornea - Google Patents
A kind of de- cell system of cornea Download PDFInfo
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- CN106267344B CN106267344B CN201510290794.4A CN201510290794A CN106267344B CN 106267344 B CN106267344 B CN 106267344B CN 201510290794 A CN201510290794 A CN 201510290794A CN 106267344 B CN106267344 B CN 106267344B
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Abstract
Cell technology field is taken off the present invention relates to cornea more particularly to a kind of cornea takes off cell system.A large amount of batch processing can be carried out, and easy to use while guarantee cornea cell free quality, it is low in cost, it creates conditions for the large-scale production and application of cornea.It is small to overcome batch processing amount in the prior art, higher cost, and it is difficult to realize the defect of large-scale production and the application of cornea.The embodiment of the present invention provides a kind of de- cell system of cornea, comprising: de- cell apparatus, oscillator device, de- cell reagent tank and injection water pot；The de- cell apparatus includes reactor tank；The oscillator device is set to the reactor tank lower part, for driving the reactor tank to be vibrated；The de- cell reagent tank and the injection water pot are used to carry out animal corneal de- cell processing, obtain de- cell cornea for alternately providing de- cell reagent and water for injection, the reactor tank into the reactor tank.
Description
Technical field
Cell technology field is taken off the present invention relates to cornea more particularly to a kind of cornea takes off cell system.
Background technique
Keratonosus is the second substantially blindness causing disease for being only second to cataract, due to the serious scarcity of people's corneal donor, angle at present
The essential therapeutic arsenals of film disease be corneal allograft, therefore seek suitable cornea substitute have to corneal transplantation it is great
Medical value.People are dedicated to developing repairing effect after good biocompatibility, immunogenicity be low and eye implantation for a long time
Good cornea substitute, after study it is found that: high molecular polymer has that biocompatibility is low, mechanical characteristic poor controllability
The shortcomings that without being able to satisfy the demand of cornea therapy.
In recent years, with the development of de- cell technology, researchers have found that de- cell cornea not only remains original tissue
Three-dimensional structure, and its main component be low immunogenicity collagen, have good biocompatibility, immunogenicity
Low and similar to people's cornea structure feature is the optimal cornea substitute of people's corneal transplantation.
Research and development, production of current cornea substitute etc. are completed in reaction flask by shaking table oscillation, processed
Treating capacity is small in journey, higher cost, limits the large-scale production and application of cornea.
Summary of the invention
In order to achieve the above objectives, the embodiment of the present invention provides a kind of de- cell system of cornea, can guarantee that cornea is de- thin
While the quality of born of the same parents, carry out a large amount of batch processing, and easy to use, it is low in cost, for cornea large-scale production and answer
With creating conditions.
The embodiment of the present invention provides a kind of de- cell system of cornea, comprising:
De- cell apparatus, oscillator device, de- cell reagent tank and injection water pot；
The de- cell apparatus includes reactor tank；
The oscillator device is set to the reactor tank lower part, for driving the reactor tank to be vibrated；
The de- cell reagent tank is connected to the reactor tank respectively with the injection water pot, for alternately to described
De- cell reagent and water for injection are provided in reactor tank, the reactor tank is used to carry out animal corneal de- cell processing, obtains
De- cell cornea.
Preferably, the oscillator device includes pedestal and pallet, and motor, the output of the motor are provided in the pedestal
End is connected with eccentric wheel, and the eccentric wheel is fixedly connected with the pallet.
Preferably, the de- cell apparatus further includes incubator, and the reactor tank is set in the incubator；The bottom
Seat is fixedly connected with the incubator, and the pallet is located in the incubator, and the eccentric wheel passes through the incubator bottom
The through-hole of setting protrudes into the incubator.
Preferably, it is provided with snap part on the pallet, for the reactor tank to be fixed.
Further, the snap part is four, and is symmetricly set on surface of the pallet relative to reactor tank
On.
Optionally, the input terminal of the de- cell reagent tank is provided with de- cell reagent make-up valve, the de- cell reagent
The output end of tank is connected to the reactor tank；
The input terminal of the injection water pot is provided with water for injection make-up valve, the output end of the injection water pot and institute
State reactor tank connection；
Wherein, it is provided with refrigerant first circulation device in the de- cell reagent tank, be arranged in the injection water pot
There is refrigerant second circulation device, refrigerant third circulator, the refrigerant first circulation are provided in the incubator
Device, the refrigerant second circulation device and the refrigerant third circulator are for the refrigerant that circulates.
Preferably, the cornea take off cell system further include refrigeration machine, the output end of the refrigeration machine respectively with the system
The entrance of cryogen first circulation device, the entrance of the refrigerant second circulation device and the refrigerant third circulator
Entrance connection；Input terminal respectively with the outlet of the refrigerant first circulation device, the refrigerant second circulation device
The outlet of outlet and the refrigerant third circulator；The output end of the refrigeration machine is followed with the refrigerant first
Between the entrance of the entrance of loop device, the entrance of the refrigerant second circulation device and the refrigerant third circulator
It is respectively arranged with three valve of one valve of refrigerant, two valve of refrigerant and refrigerant.
Optionally,
The first peristaltic pump is connected between the de- cell reagent tank and the reactor tank；
The second peristaltic pump is connected between the injection water pot and the reactor tank.
Preferably, the first filter device is provided between first peristaltic pump and the reactor tank；Described second wriggles
The second filter device is provided between pump and the reactor tank.
Optionally, it further includes waste liquid tank and diaphragm pump, the entrance and diaphragm of the waste liquid tank that the cornea, which takes off cell system,
The output end of pump is connected to, and the input terminal of the diaphragm pump is separately connected one end of sewer pipe and foamed pipe；The sewer pipe and institute
The other end for stating foamed pipe is connected separately with tapping valve and row's foam valve, and is connected to respectively with the reactor tank.
Preferably, the cornea take off cell system further include control equipment, the control equipment respectively with the de- cell
Reagent make-up valve, water for injection make-up valve, one valve of refrigerant, two valve of refrigerant, three valve of refrigerant, tapping valve and row's foam valve connect
It connects, for controlling the de- cell reagent make-up valve, water for injection make-up valve, one valve of refrigerant, two valve of refrigerant, refrigeration respectively
The on and off of three valve of agent, tapping valve and row's foam valve；
The control equipment respectively with the oscillator device, the first peristaltic pump, the second peristaltic pump, the first filter device,
Two filter devices, diaphragm pump are connected with the refrigeration machine, are vibrated for controlling the oscillator device, and it is compacted to control described first
The de- cell reagent and the water for injection are transported to the reactor tank with the second peristaltic pump by dynamic pump, control first mistake
Filter device and the second filter device are filtered degerming, and control to the de- cell reagent and water for injection that enter the reactor tank
The refrigeration machine is made respectively to freeze to the de- cell reagent tank, the injection water pot and the incubator.
The embodiment of the present invention provides a kind of cornea and takes off cell system, can will when needing to carry out cornea de- cell processing
It is swollen and the cornea of viral inactivation treatment is placed in the reactor tank, pass through the de- cell reagent tank and the injection
Water pot alternately provides de- cell reagent in right amount into the reactor tank, so that the cornea in the reactor tank is with hypertonic-hypotonic shape
Formula carries out de- cell processes, opens oscillator device, so that oscillator device vibrates, and the reactor tank is driven to be vibrated, is in
Cornea in reactor tank comes into full contact under oscillation action with de- cell reagent or water for injection, carries out de- cell, takes off cell
After process is completed, the waste liquid after de- cell is discharged, obtains de- cell cornea.It can guarantee the cell free quality of cornea
Meanwhile a large amount of batch processing is carried out, and easy to use, and it is low in cost, it creates conditions for the large-scale production and application of cornea.
It is small to overcome batch processing amount in the prior art, higher cost, and it is difficult to realize the defect of large-scale production and the application of cornea.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the structural schematic diagram that a kind of cornea provided in an embodiment of the present invention takes off cell system；
Fig. 2 is the structural schematic diagram of oscillator device provided in an embodiment of the present invention and reactor tank；
Fig. 3 is the structural schematic diagram that a kind of eccentric wheel provided in an embodiment of the present invention drives reactor tank to move reciprocatingly；
Fig. 4 is a kind of structural schematic diagram that reactor tank is fixed by snap on pallet provided in an embodiment of the present invention；
Fig. 5 is the structural schematic diagram that a kind of cornea including incubator provided in an embodiment of the present invention takes off cell system；
Fig. 6 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system；
Fig. 7 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system；
Fig. 8 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system；
Fig. 9 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system；
Figure 10 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system；
Figure 11 is the structural schematic diagram that another cornea provided in an embodiment of the present invention takes off cell system.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that, term " center ", "upper", "lower", "front", "rear", " left side ",
The orientation or positional relationship of the instructions such as " right side ", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on the figure
Orientation or positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device of indication or suggestion meaning or
Element must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
Referring to Fig. 1, for a kind of structural schematic diagram of the de- cell system of cornea provided in an embodiment of the present invention, comprising:
De- cell apparatus 1, oscillator device 2, de- cell reagent tank 3 and injection water pot 4；
The de- cell apparatus 1 includes reactor tank 12；
The oscillator device 2 is set to 12 lower part of reactor tank, for driving the reactor tank 12 to be vibrated；
The de- cell reagent tank 3 is connected to the reactor tank 12 respectively with the injection water pot 4, for alternately to
De- cell reagent and water for injection are provided in the reactor tank 12, and the reactor tank 12 is used to animal corneal is carried out at de- cell
Reason obtains de- cell cornea.
The embodiment of the present invention provides a kind of cornea and takes off cell system, can will when needing to carry out cornea de- cell processing
It is swollen and the cornea of viral inactivation treatment is placed in the reactor tank 12, pass through the de- cell reagent tank 3 and the note
It penetrates and de- cell reagent and water for injection in right amount is alternately provided into the reactor tank 12 with water pot 4, so that in the reactor tank 12
Cornea be at temperature appropriate and carry out de- cell processes in the form of hypertonic-hypotonic, oscillator device 2 is opened, so that oscillation is set
Standby 2 oscillation, and the reactor tank 12 is driven to be vibrated, the cornea in reactor tank 12 and de- cell reagent or injection
Water comes into full contact under oscillation action, and carries out de- cell, and after taking off cell processes completion, the waste liquid after de- cell is discharged,
Obtain de- cell cornea.
From the foregoing, it will be observed that carrying out de- cell, energy to cornea to be processed by physical oscillation method during whole operation
It is enough to carry out a large amount of batch processing, and easy to use while guarantee cornea cell free quality, it is low in cost, it is cornea
Large-scale production and application create conditions.It is small to overcome batch processing amount in the prior art, higher cost, and it is difficult to realize angle
The defect of large-scale production and the application of film.
Wherein, without limitation to the structure of the oscillator device 2, as long as the oscillator device 2 is able to drive the reaction
Tank 12 vibrates.In one embodiment of the invention, referring to fig. 2, the oscillator device 2 includes pedestal 21 and pallet 22,
Motor is provided in the pedestal 21, the output end of the motor is connected with eccentric wheel 23, the eccentric wheel 23 and the pallet
22 are fixedly connected.When opening oscillator device 2, the motor in the pedestal 21 is moved, and the eccentric wheel 23 is driven to occur to turn
It is dynamic, due to the axis hole secundly of eccentric wheel 23, in axis rotation, the eccentric wheel 23 drives the pallet 22 to occur
Move back and forth so that the reactor tank 12 for being located at the top of pallet 22 vibrates so that the cornea in the reactor tank 12 and
De- cell reagent oscillation carries out de- cell.
Referring to Fig. 3, without limitation to the number of the eccentric wheel 23, the eccentric wheel 23 can be 4, and be uniformly distributed
It in 22 bottom of pallet, is fixedly connected with 22 bottom of pallet, the eccentric wheel 23 and 22 bottom of pallet is consolidated
Determine mode without limitation, can be attached by bolt, can also be attached by the modes such as welding, being clamped.To described
Without limitation, the motor can be one to the number of motor, or multiple, the output end of each motor and institute
The connection of eccentric wheel 23 is stated, the eccentric wheel 23 is driven to rotate.Preferably, each described motor drives the eccentric wheel 23 synchronous
Movement.
In order to avoid when the reactor tank 12 vibrates, the reactor tank 12 is subjected to displacement above pallet 22, very
To toppling over when frequency of oscillation is higher, it is preferred that referring to fig. 4, be provided with snap part 24 on the pallet 22, be used for pair
The reactor tank 12 is fixed.
Wherein, without limitation to the number of the snap part 24, as long as the reactor tank 12 can be fixed on described
On pallet 22, it is preferred that the snap part 24 is four, and is symmetricly set on the pallet 22 relative to reactor tank
On 12 surface.In this way, the reactor tank 12 can vibrate jointly with the pallet 22 under the drive of eccentric wheel 23,
And can guarantee that the reactor tank 12 will not be subjected to displacement on the pallet 22 simultaneously, improve the safety of operation.
In one embodiment of the invention, referring to Fig. 5, the de- cell apparatus further includes incubator 11, the reactor tank 12
It is set in the incubator 11；The pedestal 21 is fixedly connected with the incubator 11, and the pallet 22 is located at the heat preservation
In case 11, the eccentric wheel 23 is protruded into the incubator 11 by the through-hole that 11 bottom of incubator is arranged.Pass through setting
Incubator 11 can cool down to the reactor tank 12, be kept the temperature, so that the cornea in the reactor tank 12 is able to maintain most
De- cell is carried out at a temperature of good.
Wherein, without limitation to the de- cell reagent tank 3 and the injection water pot 4, as long as the de- cell reagent
The de- cell reagent and water for injection entered in reactor tank 12 can be pre-chilled in tank 3 and the injection water pot 4.This
In one embodiment of invention, referring to Fig. 6, the input terminal of the de- cell reagent tank 3 is provided with de- cell reagent make-up valve a, institute
The output end for stating de- cell reagent tank 3 is connected to the reactor tank 12；The input terminal of the injection water pot 4 is provided with injection
Water make-up valve b, the output end of the injection water pot 4 are connected to the reactor tank 12；Wherein, in the de- cell reagent tank 3
It is provided with refrigerant first circulation device 31, refrigerant second circulation device 41, the guarantor are provided in the injection water pot 4
Refrigerant third circulator 111 is provided in incubator 11, the refrigerant first circulation device 31, the refrigerant second follow
Loop device 41 and the refrigerant third circulator 111 are for the refrigerant that circulates.
Using this structure, the de- cell reagent make-up valve a is opened, de- cell reagent can be conveyed to the de- cell
Reagent pot 3 opens the water for injection make-up valve b, can to the injection water pot 4 convey water for injection, when will it is described take off
Cell reagent is conveyed to before the reactor tank 12, and refrigerant is flowed through refrigerant first circulation device 31, can be to described de-
De- cell reagent in cell reagent tank 3 freezes, and enters the reactor tank 12 for the de- cell reagent and keeps de- cell
Required reaction temperature creates conditions, likewise, will make before the water for injection is alternately conveyed to the reactor tank 12
Cryogen flows through refrigerant second circulation device 41, can freeze to the water for injection in the injection water pot 4, be described
Water for injection enters temperature needed for the reactor tank 12 keeps de- cell and creates conditions, when by the de- cell reagent or note
It penetrates before being delivered to the reactor tank 12 with water, refrigerant is flowed through into the refrigerant third circulator 111, be the reaction
The temperature needed for carrying out cornea and taking off cell of tank 12 creates conditions.
Wherein, without limitation to the structure of the de- cell reagent tank 3 and the injection water pot 4, the de- cell examination
Agent tank 3 and the injection water pot 4 can be cylindrical body tank body, or cube tank body.
Without limitation to set-up mode of the refrigerant first circulation device 31 in the de- cell reagent tank 3, right
Set-up mode of the refrigerant second circulation device 41 in the injection water pot 4 without limitation, as long as making the system
It, can be to the de- cell after cryogen flows through the refrigerant first circulation device 31 and the refrigerant second circulation device 41
The water for injection in de- cell reagent and the injection water pot 4 in reagent pot 3 is freezed.
For example, the de- cell reagent tank 3 and the injection water pot 4 can be the tank body with sandwich, it is described
Refrigerant first circulation device 31 and the refrigerant second circulation device 41 can be spiral coil pipe, described spiral
Coil pipe is located in the sandwich of the de- cell reagent tank 3 and the injection water pot 4, and the refrigerant flows through described
It, can be to the de- cell reagent tank 3 and institute when refrigerant first circulation device 31 and the refrigerant second circulation device 41
The de- cell reagent and water for injection stated in injection water pot 4 are continuously exchanged heat.
Preferably, the first level electrode (not shown) and first are also provided in the de- cell reagent tank 3
Temperature electrode (not shown) respectively examines the liquid level and temperature of the de- cell reagent in the de- cell reagent tank 3
It surveys, so that the amount to the de- cell reagent in the de- cell reagent tank 3 is adjusted in time with temperature；The injection water pot
The second level electrode (not shown) and second temperature electrode (not shown) are additionally provided in 4, respectively to the injection
It is detected with the liquid level of the water for injection in water pot 4 and temperature, so as to the amount to the water for injection in the injection water pot 4
It is adjusted in time with temperature.
Also without limitation to set-up mode of the refrigerant third circulator 111 in the incubator 11, as long as
The incubator 11 can keep the temperature the reactor tank 12 in the incubator 11.Preferably, referring to Fig. 7, the system
The top in the incubator 11, the lower section of the refrigerant third circulator 111 is arranged in cryogen third circulator 111
It is provided with fan 112, when the refrigerant third circulator 31 is by refrigerant, to the refrigerant third circulator
Space refrigeration effect around 111 is more preferable, and at this moment the fan 112 enables to the air in 11 space of incubator to occur
Flowing, to freeze to the air in each corner in the incubator 11, cools down to incubator 11, is kept the temperature, so that
Reactor tank 12 in the incubator 11 can uniformly be freezed.
Wherein, without limitation to the structure of the refrigerant third circulator 111 in the incubator 11, the third system
Refrigerant cycle setting 111 can be condenser coil, and the refrigerant flows through the condenser coil, with the sky in the incubator 11
Gas exchanges heat, and achievees the purpose that freeze to the air in the incubator 11.
Without limitation to the preparation material of the incubator 11, since the reactor tank 12 is located in the incubator 11
Portion, it is preferred that the incubator 11 is made of organic glass.The situation in the incubator 11 is able to observe that in outside, just
The abnormal conditions that may occur are handled in time.
In one embodiment of the invention, referring to Fig. 8, it further includes refrigeration machine 7, the refrigeration machine that the cornea, which takes off cell system,
The 7 output end entrance with the entrance of the refrigerant first circulation device 31, the refrigerant second circulation device 41 respectively
And the entrance connection of the refrigerant third circulator；Input terminal respectively with the refrigerant first circulation device 31 go out
The outlet of mouth, the outlet of the refrigerant second circulation device 41 and the refrigerant third circulator 111；It is described
The entrance of the output end of refrigeration machine 7 and the refrigerant first circulation device 31, the refrigerant second circulation device 41 enter
Mouthful and the refrigerant third circulator 111 entrance between be respectively arranged with one valve c of refrigerant, two valve d of refrigerant and
Three valve e of refrigerant.It, can be continuously to the refrigerant first circulation device 31, the refrigeration by the way that refrigeration machine 7 is arranged
Agent second circulation device 41 and the refrigerant third circulator 111 provide refrigerant, open the one valve c of refrigerant, system
Three valve e of two valve d of cryogen and refrigerant, the refrigerant flow separately through the refrigerant first circulation device 31, the refrigerant
Second circulation device 41 and the refrigerant third circulator 111, respectively to the de- cell reagent, water for injection and
Air in incubator 11 freezes, and by the way that the one valve c of refrigerant, two valve d of refrigerant and three valve of refrigerant is arranged
E can control the one valve c of refrigerant, two valve d of refrigerant and three valve e of refrigerant to realize refrigeration, avoid manually dry
It brings bacterium into the de- cell reagent in advance or water for injection pollutes.
In a preferred embodiment of the present invention, referring to Fig. 9, connect between the de- cell reagent tank 3 and the reactor tank 12
It is connected to the first peristaltic pump 81；The second peristaltic pump 82 is connected between the injection water pot 4 and the reactor tank 12.Pass through setting
First peristaltic pump 81 can not be limited in the positional relationship between the de- cell reagent tank 3 and the reactor tank 12
In the case where, the de- cell reagent in the de- cell reagent tank 3 is conveyed under the power effect of first peristaltic pump 81
To the reactor tank 12, likewise, by the way that second peristaltic pump 82 is arranged, can the injection water pot 4 with it is described anti-
In the case where answering the positional relationship between tank 12 not limit, by the water for injection in the injection water pot 3 described second
The reactor tank 12 is delivered under the power effect of peristaltic pump 82.
In another preferred embodiment of the invention, first is provided between first peristaltic pump 81 and the reactor tank 12
Filter device 91；The second filter device 92 is provided between second peristaltic pump 82 and the reactor tank.By described in setting
First filter device 91, when the de- cell reagent is transported to the reactor tank 12 by first peristaltic pump 81, energy
Enough de- cell reagents to before entering the reactor tank 12 are filtered degerming, avoid first peristaltic pump 81 that from may bringing into
Bacterium enter the reactor tank 12 cornea to be processed is polluted；Likewise, by the way that second filter device is arranged
92, it, can be described anti-to entering when the water for injection is transported to the reactor tank 12 by second peristaltic pump 82
Water for injection before answering tank 12 is filtered degerming, and the bacterium for avoiding second peristaltic pump 82 that from may bringing into enters described anti-
Tank 12 is answered to pollute cornea to be processed.
Wherein, without limitation to first filter device 91 and the second filter device 92, first filter device 91
It can be the filter with filter core with the second filter device 92, it is preferred that the diameter of the filter core is 0.22 micron.Described
Before de- cell reagent and the water for injection enter the reactor tank 12 through first peristaltic pump 81 and the second peristaltic pump 82,
The bacterium that may be brought into retains through first filter device 91 with the filter core in second filter device 92, can be avoided thin
Bacterium enters in the reactor tank 12.
In another embodiment of the present invention, referring to Figure 10, it further includes waste liquid tank 10 and diaphragm that the cornea, which takes off cell system,
Pump 101, the entrance of the waste liquid tank 10 is connected to the output end of diaphragm pump 101, and the input terminal of the diaphragm pump 101 is separately connected
One end of sewer pipe 102 and foamed pipe 103, the sewer pipe 102 and the other end of the foamed pipe 103 are connected separately with drain
Valve f and row foam valve g, and be connected to respectively with the reactor tank 12.
Using this structure, when in reactor tank 12 cornea and de- cell reagent after setting time completes de- cell, beat
The tapping valve f and row foam valve g are opened, the waste liquid after the de- cell is extracted out from the reactor tank 12 through the diaphragm pump 101,
Wherein, a large amount of foams generated in the waste liquid are disposed in the waste liquid tank 8 by the foamed pipe 103, most of
Waste liquid is disposed in the waste liquid tank 8 by the sewer pipe 102, wherein diaphragm pump 101 can prevent the waste liquid from arranging
Reflux occurs during putting and enters in the reactor tank 12.
Wherein, without limitation to the production material of the sewer pipe 102 and the foamed pipe 103, it is preferred that the waste liquid
Pipe 102 can be stainless steel flexible hose, and the foamed pipe 103 can be silica gel hose, the sewer pipe 102 and the foamed pipe
103 are provided with ready-packaged joint with 12 connectivity part of reactor tank, when in use, facilitate installation.
In order to easy to detect, it is additionally provided with foam sensor in the reactor tank 12, foam can be detected, work as bubble
When foam reaches set amount, opens row's foam valve g and the foam in the waste liquid is discharged in time.
Wherein, without limitation to the structure of the reactor tank 12, the reactor tank 12 can be cylindrical body, cube etc..
Preferably, the reactor tank 12 is cylindrical body, and the interior of the reactor tank 12 is additionally provided with third level electrode, energy
Enough de- cell reagent amounts or injection water in the reactor tank 12 carry out real-time monitoring, in time to the reactor tank 12
It is middle to supplement de- cell reagent or water for injection, and the waste liquid amount in the reactor tank 12 can also be measured in real time,
The waste liquid in the reactor tank 12 is drained into the waste liquid tank 8 in time.
In another embodiment of the present invention, it further includes control equipment (not shown), institute that the cornea, which takes off cell system,
State control equipment respectively with the de- cell reagent make-up valve a, water for injection make-up valve b, one valve c of refrigerant, two valve of refrigerant
D, three valve e of refrigerant, tapping valve f are connect with row foam valve g, for controlling the de- cell reagent make-up valve a, water for injection respectively
The on and off of make-up valve b, one valve c of refrigerant, two valve d of refrigerant, three valve e of refrigerant, tapping valve f and row foam valve g；The control
Equipment respectively with the oscillator device 2, the first peristaltic pump 81, the second peristaltic pump 82, the first filter device 91, the second filter device
92, diaphragm pump 101 and the refrigeration machine 7 connect, and are vibrated for controlling the oscillator device 2, control described first and wriggle
The de- cell reagent and the water for injection are transported to the reactor tank 12 by pump 81 and the second peristaltic pump 82, control described the
The de- cell reagent that one filter device 91 enters the reactor tank 12 with 92 pairs of the second filter device is filtered with water for injection
Degerming, and the control refrigeration machine 7 is respectively to the de- cell reagent tank 3, the injection water pot 4 and the incubator 11
Freeze.By the way that the control equipment is arranged, it can be realized automation control, save manpower.
Further, referring to Figure 11, it further includes being connected to the de- cell reagent make-up valve a that the cornea, which takes off cell system,
De- cell reagent storage device 5, the water for injection storage device 6 being connected to the water for injection make-up valve b, and setting exist
The first lifting device 51 below the de- cell storage device 5, and be arranged in below the water for injection storage device 6
Two lifting devices 61.
Using this structure, when the de- cell reagent liquid level of the de- cell reagent storage device 5 is lower than the de- cell examination
When agent tank 3, the height of the de- cell reagent storage device 5 is adjusted by first lifting device 51, so that described de- thin
De- cell reagent in born of the same parents' reagent storage device 5 can be delivered under the effect of gravity when opening de- cell reagent make-up valve a
In the de- cell reagent tank 3, likewise, the height of the water for injection storage device 6 can also be gone up and down by described second
Device 61 is adjusted, so that the water for injection in the water for injection storage device 6 is opening the water for injection make-up valve b
When can be delivered under the effect of gravity in the injection water pot 4.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (8)
1. a kind of cornea takes off cell system characterized by comprising
De- cell apparatus, oscillator device, de- cell reagent tank and injection water pot；
The de- cell apparatus includes reactor tank；
The oscillator device is set to the reactor tank lower part, for driving the reactor tank to be vibrated；
The de- cell reagent tank is connected to the reactor tank respectively with the injection water pot, for alternately to the reaction
De- cell reagent and water for injection are provided in tank, the reactor tank is used to carry out animal corneal de- cell processing, obtains de- thin
Born of the same parents' cornea；
The first peristaltic pump is connected between the de- cell reagent tank and the reactor tank；
The second peristaltic pump is connected between the injection water pot and the reactor tank；
The first filter device is provided between first peristaltic pump and the reactor tank；Second peristaltic pump is reacted with described
The second filter device is provided between tank；
It further includes waste liquid tank and diaphragm pump that the cornea, which takes off cell system, and the entrance of the waste liquid tank and the output end of diaphragm pump connect
Logical, the input terminal of the diaphragm pump is separately connected one end of sewer pipe and foamed pipe；The sewer pipe is another with the foamed pipe
One end is connected separately with tapping valve and row's foam valve, and is connected to respectively with the reactor tank.
2. cornea according to claim 1 takes off cell system, which is characterized in that
The oscillator device includes pedestal and pallet, and motor is provided in the pedestal, and the output end of the motor is connected with partially
Heart wheel, the eccentric wheel are fixedly connected with the pallet.
3. cornea according to claim 2 takes off cell system, which is characterized in that
The de- cell apparatus further includes incubator, and the reactor tank is set in the incubator；The pedestal and the guarantor
Incubator is fixedly connected, and the pallet is located in the incubator, and the eccentric wheel passes through the through-hole that the incubator bottom is arranged
It protrudes into the incubator.
4. cornea according to claim 3 takes off cell system, which is characterized in that it is provided with snap part on the pallet,
For the reactor tank to be fixed.
5. cornea according to claim 4 takes off cell system, it is characterised in that snap part described in is four, and right
The pallet is arranged in relative on the surface of reactor tank in title.
6. cornea according to claim 3 takes off cell system, which is characterized in that the input terminal of the de- cell reagent tank is set
It is equipped with de- cell reagent make-up valve, the output end of the de- cell reagent tank is connected to the reactor tank；
The input terminal of the injection water pot is provided with water for injection make-up valve, the output end of the injection water pot and described anti-
Tank is answered to be connected to；
Wherein, it is provided with refrigerant first circulation device in the de- cell reagent tank, is provided with system in the injection water pot
Cryogen second circulation device, is provided with refrigerant third circulator in the incubator, the refrigerant first circulation device,
The refrigerant second circulation device and the refrigerant third circulator are for the refrigerant that circulates.
7. cornea according to claim 6 takes off cell system, which is characterized in that it further includes system that the cornea, which takes off cell system,
Cold, the output end of the refrigeration machine respectively with the entrance of the refrigerant first circulation device, the refrigerant second circulation
The entrance of device and the connection of the entrance of the refrigerant third circulator；Input terminal respectively with the refrigerant first circulation
The outlet of the outlet of device, the outlet of the refrigerant second circulation device and the refrigerant third circulator；
The entrance of the entrance of the output end of the refrigeration machine and the refrigerant first circulation device, the refrigerant second circulation device
And one valve of refrigerant, two valve of refrigerant and refrigerant three are respectively arranged between the entrance of the refrigerant third circulator
Valve.
8. cornea according to claim 1-7 takes off cell system, which is characterized in that the cornea takes off cell system
Further include control equipment, the control equipment respectively with the de- cell reagent make-up valve, water for injection make-up valve, refrigerant one
Valve, two valve of refrigerant, three valve of refrigerant, tapping valve are connect with row's foam valve, for controlling de- cell reagent make-up valve, injection respectively
With water make-up valve, the on and off of one valve of refrigerant, two valve of refrigerant, three valve of refrigerant, tapping valve and row's foam valve；
The control equipment respectively with the oscillator device, the first peristaltic pump, the second peristaltic pump, the first filter device, the second mistake
Filter device, diaphragm pump connects with refrigeration machine, are vibrated for controlling the oscillator device, control first peristaltic pump and the
The de- cell reagent and the water for injection are transported to the reactor tank by two peristaltic pumps, control first filter device with
Second filter device is filtered degerming, and the control system to the de- cell reagent and water for injection that enter the reactor tank
Cold respectively freezes to the de- cell reagent tank, the injection water pot and incubator.
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| CN201510290794.4A CN106267344B (en) | 2015-05-29 | 2015-05-29 | A kind of de- cell system of cornea |
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| CN201510290794.4A CN106267344B (en) | 2015-05-29 | 2015-05-29 | A kind of de- cell system of cornea |
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| CN107012085B (en) * | 2017-05-31 | 2020-01-03 | 陕西瑞盛生物科技有限公司 | Automatic fluid infusion type cell removal system and method |
| CN111658827A (en) * | 2019-12-22 | 2020-09-15 | 西安定华电子股份有限公司 | Organ stent preparation device and organ stent preparation method |
| CN113262328B (en) * | 2021-03-19 | 2023-07-14 | 宁波市第一医院 | Full-automatic and standardized biological tissue cell removing device |
| CN114870772A (en) * | 2022-03-10 | 2022-08-09 | 上海威高医疗技术发展有限公司 | Decellularization reaction kettle device and decellularization method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985051A (en) * | 2010-10-21 | 2011-03-16 | 暨南大学 | Acellular cornea or acellular corneal stroma, preparation method and application thereof |
| CN104001215A (en) * | 2014-06-13 | 2014-08-27 | 深圳艾尼尔角膜工程有限公司 | Acellular corneal stroma and preparing method for acellular corneal stroma |
| CN104001217A (en) * | 2014-06-13 | 2014-08-27 | 深圳艾尼尔角膜工程有限公司 | Decellularized method for cornea |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985051A (en) * | 2010-10-21 | 2011-03-16 | 暨南大学 | Acellular cornea or acellular corneal stroma, preparation method and application thereof |
| CN104001215A (en) * | 2014-06-13 | 2014-08-27 | 深圳艾尼尔角膜工程有限公司 | Acellular corneal stroma and preparing method for acellular corneal stroma |
| CN104001217A (en) * | 2014-06-13 | 2014-08-27 | 深圳艾尼尔角膜工程有限公司 | Decellularized method for cornea |
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