CN106267344A - A kind of cornea takes off cell system - Google Patents
A kind of cornea takes off cell system Download PDFInfo
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- CN106267344A CN106267344A CN201510290794.4A CN201510290794A CN106267344A CN 106267344 A CN106267344 A CN 106267344A CN 201510290794 A CN201510290794 A CN 201510290794A CN 106267344 A CN106267344 A CN 106267344A
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- cell
- cold
- producing medium
- retort
- cornea
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Abstract
The present invention relates to cornea and take off cell technology field, particularly relate to a kind of cornea and take off cell system.Substantial amounts of batch processing can be carried out while ensureing the cell free quality of cornea, and easy to use, and with low cost, large-scale production and application for cornea create conditions.Overcome batch processing amount in prior art little, relatively costly, and it is difficult to the large-scale production of cornea and the defect of application.The embodiment of the present invention provides a kind of cornea to take off cell system, including: de-cell apparatus, oscillator device, de-cell reagent tank and injection water pot；Described de-cell apparatus includes retort；Described oscillator device is arranged at described retort bottom, is used for driving described retort to vibrate；Described de-cell reagent tank and described injection water pot process for animal corneal carries out de-cell for the de-cell reagent of offer in described retort alternately and water for injection, described retort, it is thus achieved that take off cell cornea.
Description
Technical field
The present invention relates to cornea and take off cell technology field, particularly relate to a kind of cornea and take off cell line
System.
Background technology
Keratopathy is to be only second to cataractous second substantially blindness causing disease, tight due to people's corneal donor
Weight scarcity, the essential therapeutic arsenals of current keratopathy is corneal allograft, and it is suitable therefore to seek
Cornea substitute corneal transplant have great medical value.People are devoted to for a long time
Development good biocompatibility, immunogenicity are low, and the cornea that after eye implantation, repairing effect is good
Substitute, it is found that through research: high molecular polymer has that biocompatibility is low, mechanics
Controlled properties difference shortcoming and the demand of cornea therapy can not be met.
In recent years, along with the development of de-cell technology, researchers find de-cell cornea not only
Remain former organized 3-D solid structure, and it is mainly composed of the glue of reduced immunogenicity
Former albumen, has the spy that good biocompatibility, immunogenicity are low and similar to people's cornea structure
Point, for the cornea substitute that people's corneal transplantation is optimal.
The research and development of current cornea substitute, production etc. are all vibrated by shaking table in reaction bulb
Completing, in processing procedure, treating capacity is little, relatively costly, limits the large-scale production of cornea
And application.
Summary of the invention
For reaching above-mentioned purpose, the embodiment of the present invention provides a kind of cornea to take off cell system, it is possible to
While ensureing the cell free quality of cornea, carry out substantial amounts of batch processing, and easy to use,
With low cost, large-scale production and application for cornea create conditions.
The embodiment of the present invention provides a kind of cornea to take off cell system, including:
De-cell apparatus, oscillator device, de-cell reagent tank and injection water pot；
Described de-cell apparatus includes retort；
Described oscillator device is arranged at described retort bottom, is used for driving described retort to carry out
Vibration；
Described de-cell reagent tank connects with described retort respectively with described injection water pot, uses
In the de-cell reagent of offer in described retort alternately and water for injection, described retort is used
Process in animal corneal being carried out de-cell, it is thus achieved that de-cell cornea.
Preferably, described oscillator device includes base and pallet, is provided with motor in described base,
The outfan of described motor connects eccentric, and described eccentric is fixing with described pallet to be connected.
Preferably, also to include that couveuse, described retort are arranged at described for described de-cell apparatus
In couveuse；Described base is fixing with described couveuse to be connected, and described pallet is positioned at described insulation
In case, described eccentric stretches into described couveuse by the through hole arranged bottom described couveuse
In.
Preferably, described pallet is provided with snap part, solid for described retort is carried out
Fixed.
Further, described snap part is four, and it is relative to be symmetricly set on described pallet
On the surface of retort.
Optionally, the input of described de-cell reagent tank is provided with de-cell reagent make-up valve,
The outfan of described de-cell reagent tank connects with described retort；
The input of described injection water pot is provided with water for injection make-up valve, described water for injection
The outfan of tank connects with described retort；
Wherein, described de-cell reagent tank is provided with cold-producing medium the first circulating device, described note
Penetrate with water pot is provided with cold-producing medium the second circulating device, described couveuse is provided with cold-producing medium
3rd circulating device, described cold-producing medium the first circulating device, described cold-producing medium the second circulating device
It is used for the cold-producing medium that circulates with described cold-producing medium the 3rd circulating device.
Preferably, described cornea takes off cell system and also includes refrigeration machine, the output of described refrigeration machine
End respectively with entrance, described cold-producing medium second circulating device of described cold-producing medium the first circulating device
Entrance and described cold-producing medium the 3rd circulating device entrance connection；Input is respectively with described
The outlet of cold-producing medium the first circulating device, the outlet of described cold-producing medium the second circulating device and institute
State the outlet of cold-producing medium the 3rd circulating device；The outfan of described refrigeration machine and described refrigeration
The entrance of agent the first circulating device, the entrance of described cold-producing medium the second circulating device and described system
Be respectively arranged with between the entrance of cryogen the 3rd circulating device cold-producing medium one valve, cold-producing medium two valve and
Cold-producing medium three valve.
Optionally,
It is connected between described de-cell reagent tank and described retort and has the first peristaltic pump；
It is connected between described injection water pot and described retort and has the second peristaltic pump.
Preferably, the first filtration dress it is provided with between described first peristaltic pump and described retort
Put；It is provided with the second defecator between described second peristaltic pump and described retort.
Optionally, described cornea takes off cell system and also includes waste liquid tank and membrane pump, described waste liquid
The entrance of tank connects with barrier film delivery side of pump, and the input of described membrane pump connects waste liquid respectively
Pipe and one end of foamed pipe；Described sewer pipe is connected to row with the other end of described foamed pipe
Liquid valve and row's foam valve, and connect with described retort respectively.
Preferably, described cornea takes off cell system and also includes that control equipment, described control equipment divide
Not with described de-cell reagent make-up valve, water for injection make-up valve, cold-producing medium one valve, cold-producing medium
Two valves, cold-producing medium three valve, tapping valve are connected with row's foam valve, for controlling described de-cell respectively
Reagent make-up valve, water for injection make-up valve, cold-producing medium one valve, cold-producing medium two valve, cold-producing medium three
Valve, tapping valve and the on an off of row's foam valve；
Described control equipment respectively with described oscillator device, the first peristaltic pump, the second peristaltic pump,
First defecator, the second defecator, membrane pump and described refrigeration machine connect, and are used for controlling
Described oscillator device is vibrated, and controls described first peristaltic pump and the second peristaltic pump by described de-
Cell reagent and described water for injection are transported to described retort, control described first defecator
With the second defecator, the de-cell reagent entering described retort was carried out with water for injection
Filter bacterium, and control described refrigeration machine respectively to described de-cell reagent tank, described injection
Water pot and described couveuse freeze.
The embodiment of the present invention provides a kind of cornea to take off cell system, when needs corneal carries out de-thin
When born of the same parents process, can will be positioned in described retort with viral inactivation treatment cornea through swelling,
Offer in described retort is replaced by described de-cell reagent tank and described injection water pot
Appropriate de-cell reagent so that the cornea in described retort with height ooze-hypotonic form takes off
Cell processes, opens oscillator device so that oscillator device is vibrated, and drives described retort to enter
Row vibration, the cornea being in retort and de-cell reagent or water for injection are at oscillation action
Under be fully contacted, carry out de-cell, after de-cell processes completes, by the waste liquid after de-cell
Discharge, it is thus achieved that de-cell cornea.Can carry out while ensureing the cell free quality of cornea
Substantial amounts of batch processing, and easy to use, with low cost, for cornea large-scale production and should
With creating conditions.Overcome batch processing amount in prior art little, relatively costly, and it is difficult to reality
The large-scale production of existing cornea and the defect of application.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below
The accompanying drawing used required in embodiment or description of the prior art will be briefly described, aobvious and
Easily insight, the accompanying drawing in describing below is only some embodiments of the present invention, for this area
From the point of view of those of ordinary skill, on the premise of not paying creative work, it is also possible to according to these
Accompanying drawing obtains other accompanying drawing.
Fig. 1 takes off the structural representation of cell system for a kind of cornea that the embodiment of the present invention provides；
The oscillator device that Fig. 2 provides for the embodiment of the present invention and the structural representation of retort；
Fig. 3 drives retort to move reciprocatingly for a kind of eccentric that the embodiment of the present invention provides
Structural representation；
A kind of retort that Fig. 4 provides for the embodiment of the present invention is fixed by snap on pallet
Structural representation；
A kind of cornea including couveuse that Fig. 5 provides for the embodiment of the present invention takes off cell system
Structural representation；
Fig. 6 takes off the structural representation of cell system for the another kind of cornea that the embodiment of the present invention provides
Figure；
Fig. 7 takes off the structural representation of cell system for the another kind of cornea that the embodiment of the present invention provides
Figure；
Fig. 8 takes off the structural representation of cell system for the another kind of cornea that the embodiment of the present invention provides
Figure；
Fig. 9 takes off the structural representation of cell system for the another kind of cornea that the embodiment of the present invention provides
Figure；
Figure 10 takes off the structural representation of cell system for the another kind of cornea that the embodiment of the present invention provides
Figure；
Figure 11 takes off the structural representation of cell system for another cornea that the embodiment of the present invention provides
Figure.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that described embodiment is only the present invention one
Divide embodiment rather than whole embodiments.Based on the embodiment in the present invention, this area is general
The every other embodiment that logical technical staff is obtained under not making creative work premise,
Broadly fall into the scope of protection of the invention.
In describing the invention, it is to be understood that term " " center ", " on ", D score,
"front", "rear", "left", "right", " vertically ", " level ", " top ",
Orientation or the position relationship of the instruction such as " end ", " interior ", " outward " are based on shown in the drawings
Orientation or position relationship, be for only for ease of the description present invention and simplify description rather than instruction
Or the hint device of indication or element must have specific orientation, with specific azimuth configuration and
Operation, is therefore not considered as limiting the invention.
Seeing Fig. 1, a kind of cornea provided for the embodiment of the present invention takes off the structure of cell system and shows
It is intended to, including:
De-cell apparatus 1, oscillator device 2, de-cell reagent tank 3 and injection water pot 4；
Described de-cell apparatus 1 includes retort 12；
Described oscillator device 2 is arranged at described retort 12 bottom, is used for driving described reaction
Tank 12 vibrates；
Described de-cell reagent tank 3 and described injection water pot 4 respectively with described retort 12
Connection, for the de-cell reagent of offer in described retort 12 alternately and water for injection,
Described retort 12 processes for animal corneal carries out de-cell, it is thus achieved that de-cell cornea.
The embodiment of the present invention provides a kind of cornea to take off cell system, when needs corneal carries out de-thin
When born of the same parents process, can will be positioned over described retort 12 through swelling with viral inactivation treatment cornea
In, replaced to described retort with described injection water pot 4 by described de-cell reagent tank 3
Appropriate de-cell reagent and water for injection is provided so that the cornea in described retort 12 in 12
Be at a temperature of suitably with height ooze-hypotonic form carries out de-cell processes, opens oscillator device
2 so that oscillator device 2 is vibrated, and drive described retort 12 to vibrate, it is in reaction
Cornea in tank 12 is fully contacted under oscillation action with de-cell reagent or water for injection,
And carry out de-cell, after de-cell processes completes, the waste liquid after de-cell is discharged, it is thus achieved that
De-cell cornea.
From the foregoing, it will be observed that in whole operating process, by physical oscillation method to pending angle
Film carries out de-cell, it is possible to while ensureing the cell free quality of cornea, carry out substantial amounts of batch
Process, and easy to use, and with low cost, bar is created in large-scale production and application for cornea
Part.Overcome batch processing amount in prior art little, relatively costly, and it is difficult to cornea
Large-scale production and the defect of application.
Wherein, the structure of described oscillator device 2 is not limited, if described oscillator device 2
Described retort 12 can be driven to vibrate.In one embodiment of the invention, see
Fig. 2, described oscillator device 2 includes base 21 and pallet 22, is provided with in described base 21
Motor, the outfan of described motor connects eccentric 23, described eccentric 23 and described torr
Dish 22 is fixing to be connected.When opening oscillator device 2, the motor in described base 21 moves,
Described eccentric 23 is driven to rotate, due to the axis hole secundly of eccentric 23, therefore,
When axle rotates, described eccentric 23 drives described pallet 22 to move back and forth so that position
Retort 12 above pallet 22 is vibrated, so that in described retort 12
Cornea carries out de-cell with the vibration of de-cell reagent.
Seeing Fig. 3, do not limit the number of described eccentric 23, described eccentric 23 can
Think 4, and be uniformly distributed in bottom described pallet 22, fix bottom described pallet 22
Connect, the fixed form bottom described eccentric 23 and described pallet 22 is not limited, can
To be attached by bolt, it is also possible to be attached by modes such as welding, clampings.To institute
The number stating motor does not limits, and described motor can be one, it is also possible to for multiple, each
The outfan of individual described motor is connected with described eccentric 23, drives described eccentric 23 to rotate.
Preferably, eccentric 23 described in each described driven by motor is synchronized with the movement.
In order to avoid when described retort 12 occurs vibration, described retort 12 is at pallet
22 occur over displacement, even topple over when frequency of oscillation is higher, it is preferred that see
Fig. 4, described pallet 22 is provided with snap part 24, for carrying out described retort 12
Fixing.
Wherein, the number of described snap part 24 is not limited, if can by described instead
Tank 12 is answered to be fixed on described pallet 22, it is preferred that described snap part 24 is four
Individual, and be symmetricly set on the described pallet 22 surface relative to retort 12.So,
Under the drive of eccentric 23, described retort 12 can occur jointly with described pallet 22
Vibration, and ensure that described retort 12 will not be subjected to displacement on described pallet 22 simultaneously,
Improve the safety of operation.
In one embodiment of the invention, seeing Fig. 5, described de-cell apparatus also includes couveuse
11, described retort 12 is arranged in described couveuse 11；Described base 21 and described guarantor
Incubator 11 is fixing to be connected, and described pallet 22 is positioned at described couveuse 11, described eccentric
23 are stretched in described couveuse 11 by the through hole arranged bottom described couveuse 11.Pass through
Couveuse 11 is set, it is possible to described retort 12 is lowered the temperature, is incubated so that described instead
Answer the cornea in tank 12 can be maintained at a temperature of optimal and carry out de-cell.
Wherein, described de-cell reagent tank 3 is not limited with described injection water pot 4, only
Want the described de-cell reagent tank 3 can be to entering in retort 12 with described injection water pot 4
De-cell reagent and water for injection carry out pre-cooling.In one embodiment of the invention, see
Fig. 6, the input of described de-cell reagent tank 3 is provided with de-cell reagent make-up valve a, institute
The outfan stating de-cell reagent tank 3 connects with described retort 12；Described injection water pot
The input of 4 is provided with water for injection make-up valve b, the outfan of described injection water pot 4 with
Described retort 12 connects；Wherein, described de-cell reagent tank 3 is provided with cold-producing medium
One circulating device 31, is provided with cold-producing medium the second circulating device 41 in described injection water pot 4,
Described couveuse 11 is provided with cold-producing medium the 3rd circulating device 111, described cold-producing medium first
Circulating device 31, described cold-producing medium the second circulating device 41 and described cold-producing medium the 3rd circulation dress
Put 111 for the cold-producing medium that circulates.
Use this structure, open described de-cell reagent make-up valve a, can be by de-cell reagent
Flow to described de-cell reagent tank 3, open described water for injection make-up valve b, can be to institute
State injection water pot 4 and carry water for injection, when described de-cell reagent is flowed to described reaction
Before tank 12, cold-producing medium is flowed through cold-producing medium the first circulating device 31, it is possible to described de-thin
De-cell reagent in born of the same parents' reagent pot 3 freezes, described instead for the entrance of described de-cell reagent
Reaction temperature needed for answering tank 12 to keep de-cell creates conditions, same, when alternately by institute
State before water for injection flows to described retort 12, cold-producing medium is flowed through cold-producing medium second and follows
Loop device 41, it is possible to freeze the water for injection in described injection water pot 4, for institute
State the temperature needed for water for injection enters the de-cell of described retort 12 holding to create conditions, when
Before described de-cell reagent or water for injection are delivered to described retort 12, will refrigeration
Agent flows through described cold-producing medium the 3rd circulating device 111, is carrying out cornea for described retort 12
Temperature needed for de-cell creates conditions.
Wherein, the structure of described de-cell reagent tank 3 and described injection water pot 4 is not limited
Fixed, described de-cell reagent tank 3 and described injection water pot 4 can be cylinder tank body, also
It can be cube tank body.
To the setting in described de-cell reagent tank 3 of described cold-producing medium the first circulating device 31
Mode does not limits, to described cold-producing medium the second circulating device 41 at described injection water pot 4
In set-up mode do not limit, follow as long as making described cold-producing medium flow through described cold-producing medium first
After loop device 31 and described cold-producing medium the second circulating device 41, it is possible to described de-cell reagent
De-cell reagent in tank 3 and the water for injection in described injection water pot 4 carry out refrigeration i.e.
Can.
Such as, described de-cell reagent tank 3 and described injection water pot 4 can be to have interlayer
The tank body of structure, described cold-producing medium the first circulating device 31 and described cold-producing medium second circulate dress
Putting 41 can be spiral coil pipe, and described spiral coil pipe lays respectively at described de-cell
In the sandwich of reagent pot 3 and described injection water pot 4, described cold-producing medium flows through described system
When cryogen the first circulating device 31 and described cold-producing medium the second circulating device 41, it is possible to described
De-cell reagent and water for injection in de-cell reagent tank 3 and described injection water pot 4 are carried out
Continuously heat exchange.
Preferably, the first level electrode (figure it is also provided with in described de-cell reagent tank 3
Not shown in) and the first temperature electrode (not shown), respectively to described de-cell reagent
Liquid level and the temperature of the de-cell reagent in tank 3 detect, in order to described de-cell reagent
The amount of the de-cell reagent in tank 3 adjusts in time with temperature；In described injection water pot 4
It is additionally provided with the second level electrode (not shown) and the second temperature electrode (not shown),
Liquid level and temperature to the water for injection in described injection water pot 4 detect respectively, in order to
The amount of the water for injection in described injection water pot 4 is adjusted in time with temperature.
To described cold-producing medium the 3rd circulating device 111 set-up mode in described couveuse 11
Do not limit, as long as described couveuse 11 can be to the retort in described couveuse 11
12 carry out being incubated.Preferably, Fig. 7, described cold-producing medium the 3rd circulating device 111 are seen
It is arranged on the top in described couveuse 11, described cold-producing medium the 3rd circulating device 111 times
Side is provided with fan 112, when described cold-producing medium the 3rd circulating device 31 is through cold-producing medium,
More preferable to the space refrigeration effect around described cold-producing medium the 3rd circulating device 111, at this moment described
The air that fan 112 enables in described couveuse 11 space flows, thus to institute
The air stating couveuse 11 each corner interior freezes, and couveuse 11 is lowered the temperature, protected
Temperature so that the retort 12 in described couveuse 11 can uniformly be freezed.
Wherein, to the structure of cold-producing medium the 3rd circulating device 111 in described couveuse 11 not
Limiting, it can be condenser coil that described 3rd refrigerant cycle arranges 111, described cold-producing medium
Flow through described condenser coil, carry out heat exchange with the air in described couveuse 11, reach institute
State the purpose that the air in couveuse 11 carries out freezing.
The preparation material of described couveuse 11 is not limited, owing to described retort 12 is positioned at
Described couveuse 11 is internal, it is preferred that described couveuse 11 is made for lucite.Outside
Portion is able to observe that the situation in described couveuse 11, it is simple in time to contingent exception
Situation processes.
In one embodiment of the invention, seeing Fig. 8, described cornea takes off cell system and also includes system
Cold 7, the outfan of described refrigeration machine 7 respectively with described cold-producing medium the first circulating device 31
Entrance, the entrance of described cold-producing medium the second circulating device 41 and described cold-producing medium the 3rd follow
The entrance connection of loop device；Input respectively with the going out of described cold-producing medium the first circulating device 31
Mouth, the outlet of described cold-producing medium the second circulating device 41 and described cold-producing medium the 3rd circulation dress
Put the outlet of 111；The outfan of described refrigeration machine 7 circulates dress with described cold-producing medium first
Put the entrance of 31, the entrance of described cold-producing medium the second circulating device 41 and described cold-producing medium
Cold-producing medium one valve c, cold-producing medium two valve d it is respectively arranged with between the entrance of three circulating device 111
With cold-producing medium three valve e.By arranging refrigeration machine 7, it is possible to continuously to described cold-producing medium
First circulating device 31, described cold-producing medium the second circulating device 41 are followed with described cold-producing medium the 3rd
Loop device 111 provides cold-producing medium, opens described cold-producing medium one valve c, cold-producing medium two valve d and system
Cryogen three valve e, described cold-producing medium flows separately through described cold-producing medium the first circulating device 31, described
Cold-producing medium the second circulating device 41 and described cold-producing medium the 3rd circulating device 111 are the most right
Air in described de-cell reagent, water for injection and couveuse 11 freezes, and
By arranging described cold-producing medium one valve c, cold-producing medium two valve d and cold-producing medium three valve e, it is possible to right
Described cold-producing medium one valve c, cold-producing medium two valve d and cold-producing medium three valve e are controlled realizing system
Cold, it is to avoid antibacterial is brought into described de-cell reagent by manual intervention or water for injection causes dirt
Dye.
In a preferred embodiment of the present invention, see Fig. 9, described de-cell reagent tank 3 and institute
State to connect between retort 12 and have the first peristaltic pump 81；Described injection water pot 4 with described instead
Answer to connect between tank 12 and have the second peristaltic pump 82.By arranging described first peristaltic pump 81, energy
Enough position relationships between described de-cell reagent tank 3 and described retort 12 do not limit
In the case of, by the de-cell reagent in described de-cell reagent tank 3 at described first peristaltic pump
Described retort 12 it is delivered under the dynamic action of 81, same, by arranging described second
Peristaltic pump 82, it is possible to the position between described injection water pot 4 and described retort 12 is closed
In the case of system does not limit, by the water for injection in described injection water pot 3 described second
Described retort 12 it is delivered under the dynamic action of peristaltic pump 82.
In the another preferred embodiment of the present invention, described first peristaltic pump 81 and described retort
The first defecator 91 it is provided with between 12；Described second peristaltic pump 82 and described retort it
Between be provided with the second defecator 92.By arranging described first defecator 91, when described
De-cell reagent is when described first peristaltic pump 81 is transported to described retort 12, it is possible to
The de-cell reagent entered before described retort 12 is carried out filtration sterilization, it is to avoid described the
The antibacterial that one peristaltic pump 81 may be brought into enters described retort 12 and causes pending cornea
Pollute；Same, by arranging described second defecator 92, when described water for injection warp
Cross described second peristaltic pump 82 when being transported to described retort 12, it is possible to enter described instead
The water for injection before tank 12 is answered to carry out filtration sterilization, it is to avoid described second peristaltic pump 82 may
The antibacterial brought into enters described retort 12 and pollutes pending cornea.
Wherein, described first defecator 91 is not limited with the second defecator 92, institute
State the first defecator 91 and the second defecator 92 can be the filter with filter element, excellent
Choosing, a diameter of 0.22 micron of described filter element.In described de-cell reagent and described injection
With water before described first peristaltic pump 81 and the second peristaltic pump 82 enter described retort 12,
The antibacterial that may bring into is in described first defecator 91 and described second defecator 92
Filter element retains, it is possible to avoid antibacterial to enter in described retort 12.
In another embodiment of the present invention, seeing Figure 10, described cornea takes off cell system and also wraps
Including waste liquid tank 10 and membrane pump 101, the entrance of described waste liquid tank 10 is defeated with membrane pump 101
Going out end connection, the input of described membrane pump 101 connects sewer pipe 102 and foamed pipe respectively
One end of 103, described sewer pipe 102 is connected to the other end of described foamed pipe 103
Tapping valve f and row foam valve g, and connect with described retort 12 respectively.
Use this structure, when the cornea in retort 12 and de-cell reagent are complete in the time of setting
After becoming de-cell, open described tapping valve f and row foam valve g, the waste liquid after described de-cell
Extract out from described retort 12 through described membrane pump 101, wherein, described waste liquid produces
A large amount of foams be disposed in described waste liquid tank 8 by described foamed pipe 103, major part
Waste liquid is disposed in described waste liquid tank 8 by described sewer pipe 102, wherein, and membrane pump
101 are prevented from described waste liquid occurs backflow to enter described retort 12 in discharge process
In.
Wherein, the making material of described sewer pipe 102 with described foamed pipe 103 is not limited,
Preferably, described sewer pipe 102 can be stainless steel flexible hose, and described foamed pipe 103 can be
Silica gel hose, described sewer pipe 102 connects with described retort 12 with described foamed pipe 103
Place is provided with ready-packaged joint, in use, and convenient installation.
In order to easy to detect, it is additionally provided with foam sensor in described retort 12, it is possible to right
Foam detects, and when foam reaches set amount, opens described row foam valve g by described waste liquid
In foam discharge in time.
Wherein, not limiting the structure of described retort 12, described retort 12 can be
Cylinder, cube etc..
Preferably, described retort 12 also sets up in being cylinder, and described retort 12
There is the 3rd level electrode, it is possible to the de-cell reagent amount in described retort 12 or injection
Water consumption is monitored in real time, supplement in described retort 12 in time de-cell reagent or
Water for injection, and also the waste liquid amount in described retort 12 can be detected in real time,
In time the waste liquid in described retort 12 is drained in described waste liquid tank 8.
In another embodiment of the present invention, described cornea takes off cell system and also includes control equipment
(not shown), described control equipment respectively with described de-cell reagent make-up valve a, note
Penetrate with water make-up valve b, cold-producing medium one valve c, cold-producing medium two valve d, cold-producing medium three valve e, discharge opeing
Valve f is connected with row foam valve g, for controlling described de-cell reagent make-up valve a, injection respectively
With water make-up valve b, cold-producing medium one valve c, cold-producing medium two valve d, cold-producing medium three valve e, tapping valve
The on an off of f and row foam valve g；Described control equipment respectively with described oscillator device 2, first
Peristaltic pump the 81, second peristaltic pump the 82, first defecator the 91, second defecator 92, every
Membrane pump 101 and described refrigeration machine 7 connect, and are used for controlling described oscillator device 2 and vibrate,
Control described first peristaltic pump 81 and the second peristaltic pump 82 by described de-cell reagent and described note
Penetrate and be transported to described retort 12 with water, control described first defecator 91 and second and filter
The de-cell reagent entering described retort 12 was filtered by device 92 with water for injection
Bacterium, and control described refrigeration machine 7 respectively to described de-cell reagent tank 3, described injection
Water pot 4 and described couveuse 11 freeze.By arranging described control equipment, it is possible to real
Existing Automated condtrol, saves manpower.
Further, seeing Figure 11, described cornea takes off cell system and also includes de-thin with described
The de-cell reagent storage device 5 of born of the same parents' reagent make-up valve a connection, with described water for injection fluid infusion
The water for injection storage device 6 of valve b connection, and it is arranged on described de-cell storage device 5
The first following lowering or hoisting gear 51, and be arranged on below described water for injection storage device 6
Second lowering or hoisting gear 61.
Use this structure, when the de-cell reagent liquid level of described de-cell reagent storage device 5 is low
When described de-cell reagent tank 3, regulated described de-thin by described first lowering or hoisting gear 51
The height of born of the same parents' reagent storage device 5 so that taking off in described de-cell reagent storage device 5 is thin
Born of the same parents' reagent can be delivered to described under gravity when opening de-cell reagent make-up valve a
In de-cell reagent tank 3, same, the height of described water for injection storage device 6 can also
It is adjusted by described second lowering or hoisting gear 61 so that described water for injection storage device 6
In water for injection can be the most defeated when opening described water for injection make-up valve b
Deliver in described injection water pot 4.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is also
Being not limited to this, any those familiar with the art is at the technology model that the invention discloses
In enclosing, change can be readily occurred in or replace, all should contain within protection scope of the present invention.
Therefore, protection scope of the present invention should be as the criterion with described scope of the claims.
Claims (11)
1. a cornea takes off cell system, it is characterised in that including:
De-cell apparatus, oscillator device, de-cell reagent tank and injection water pot；
Described de-cell apparatus includes retort；
Described oscillator device is arranged at described retort bottom, is used for driving described retort to shake
Swing；
Described de-cell reagent tank connects with described retort respectively with described injection water pot, is used for
Providing in described retort alternately takes off cell reagent and water for injection, and described retort is for right
Animal corneal carries out de-cell and processes, it is thus achieved that de-cell cornea.
Cornea the most according to claim 1 takes off cell system, it is characterised in that
Described oscillator device includes base and pallet, is provided with motor, described motor in described base
Outfan connect and have an eccentric, described eccentric is fixed with described pallet and is connected.
Cornea the most according to claim 2 takes off cell system, it is characterised in that
Described de-cell apparatus also includes that couveuse, described retort are arranged in described couveuse；
Described base and described couveuse are fixing to be connected, and described pallet is positioned at described couveuse, described partially
Heart wheel is stretched in described couveuse by the through hole arranged bottom described couveuse.
4. take off cell system according to the cornea described in Claims 2 or 3, it is characterised in that described
Snap part it is provided with, for described retort is fixed on pallet.
Cornea the most according to claim 4 takes off cell system, it is characterised in that. described buckle
Parts are four, and are symmetricly set on described pallet relative on the surface of retort.
Cornea the most according to claim 3 takes off cell system, it is characterised in that described de-thin
The input of born of the same parents' reagent pot is provided with de-cell reagent make-up valve, the output of described de-cell reagent tank
End connects with described retort；
The input of described injection water pot is provided with water for injection make-up valve, described injection water pot
Outfan connect with described retort；
Wherein, described de-cell reagent tank is provided with cold-producing medium the first circulating device, described injection
With water pot is provided with cold-producing medium the second circulating device, described couveuse is provided with cold-producing medium the 3rd
Circulating device, described cold-producing medium the first circulating device, described cold-producing medium the second circulating device are with described
Cold-producing medium the 3rd circulating device is used for the cold-producing medium that circulates.
Cornea the most according to claim 6 takes off cell system, it is characterised in that described cornea
De-cell system also includes refrigeration machine, the outfan of described refrigeration machine respectively with described cold-producing medium first
The entrance of circulating device, the entrance of described cold-producing medium the second circulating device and described cold-producing medium the 3rd
The entrance connection of circulating device；Input respectively with the outlet of described cold-producing medium the first circulating device,
The outlet of described cold-producing medium the second circulating device and the outlet of described cold-producing medium the 3rd circulating device are even
Logical；The outfan of described refrigeration machine and the entrance of described cold-producing medium the first circulating device, described refrigeration
Set respectively between the entrance of agent the second circulating device and the entrance of described cold-producing medium the 3rd circulating device
It is equipped with cold-producing medium one valve, cold-producing medium two valve and cold-producing medium three valve.
Cornea the most according to claim 1 takes off cell system, it is characterised in that
It is connected between described de-cell reagent tank and described retort and has the first peristaltic pump；
It is connected between described injection water pot and described retort and has the second peristaltic pump.
Cornea the most according to claim 8 takes off cell system, it is characterised in that described first
The first defecator it is provided with between peristaltic pump and described retort；Described second peristaltic pump is with described
The second defecator it is provided with between retort.
Cornea the most according to claim 1 takes off cell system, it is characterised in that also include
Waste liquid tank and membrane pump, the entrance of described waste liquid tank connects with barrier film delivery side of pump, described barrier film
The input of pump connects one end of sewer pipe and foamed pipe respectively；Described sewer pipe and described foamed pipe
The other end be connected to tapping valve and row's foam valve, and connect with described retort respectively.
11. take off cell system according to the cornea described in any one of claim 1-10, it is characterised in that
Described cornea takes off cell system and also includes control equipment, described control equipment respectively with described de-cell
Reagent make-up valve, water for injection make-up valve, cold-producing medium one valve, cold-producing medium two valve, cold-producing medium three valve,
Tapping valve is connected with row's foam valve, for controlling de-cell reagent make-up valve, water for injection fluid infusion respectively
Valve, cold-producing medium one valve, cold-producing medium two valve, cold-producing medium three valve, tapping valve and the on an off of row's foam valve；
Described control equipment respectively with described oscillator device, the first peristaltic pump, the second peristaltic pump,
One defecator, the second defecator, membrane pump and described refrigeration machine connect, and are used for controlling described
Oscillator device is vibrated, and controls described first peristaltic pump and the second peristaltic pump and is tried by described de-cell
Agent and described water for injection are transported to described retort, control described first defecator and the second mistake
Filter device carries out filtration sterilization to the de-cell reagent entering described retort with water for injection, and
Control described refrigeration machine respectively to described de-cell reagent tank, described injection water pot and described insulation
Case freezes.
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| CN201510290794.4A CN106267344B (en) | 2015-05-29 | 2015-05-29 | A kind of de- cell system of cornea |
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| CN107012085A (en) * | 2017-05-31 | 2017-08-04 | 陕西瑞盛生物科技有限公司 | A kind of automatic liquid supply formula takes off cell system and method |
| CN111658827A (en) * | 2019-12-22 | 2020-09-15 | 西安定华电子股份有限公司 | Organ stent preparation device and organ stent preparation method |
| CN113262328A (en) * | 2021-03-19 | 2021-08-17 | 宁波市第一医院 | Full-automatic and standardized biological tissue decellularization device |
| CN114870772A (en) * | 2022-03-10 | 2022-08-09 | 上海威高医疗技术发展有限公司 | Decellularization reaction kettle device and decellularization method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012085A (en) * | 2017-05-31 | 2017-08-04 | 陕西瑞盛生物科技有限公司 | A kind of automatic liquid supply formula takes off cell system and method |
| CN107012085B (en) * | 2017-05-31 | 2020-01-03 | 陕西瑞盛生物科技有限公司 | Automatic fluid infusion type cell removal system and method |
| CN111658827A (en) * | 2019-12-22 | 2020-09-15 | 西安定华电子股份有限公司 | Organ stent preparation device and organ stent preparation method |
| CN113262328A (en) * | 2021-03-19 | 2021-08-17 | 宁波市第一医院 | Full-automatic and standardized biological tissue decellularization device |
| CN113262328B (en) * | 2021-03-19 | 2023-07-14 | 宁波市第一医院 | Full-automatic and standardized biological tissue cell removing device |
| CN114870772A (en) * | 2022-03-10 | 2022-08-09 | 上海威高医疗技术发展有限公司 | Decellularization reaction kettle device and decellularization method |
| CN114870772B (en) * | 2022-03-10 | 2024-07-12 | 上海威高医疗技术发展有限公司 | Decellularization reaction kettle device and decellularization method |
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| CN106267344B (en) | 2019-04-05 |
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