CN108795745A - A kind of cell culture apparatus of biotechnology temperature-adjustable - Google Patents
A kind of cell culture apparatus of biotechnology temperature-adjustable Download PDFInfo
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- CN108795745A CN108795745A CN201810706996.6A CN201810706996A CN108795745A CN 108795745 A CN108795745 A CN 108795745A CN 201810706996 A CN201810706996 A CN 201810706996A CN 108795745 A CN108795745 A CN 108795745A
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- wall
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to biotechnology technical fields, especially a kind of cell culture apparatus of biotechnology temperature-adjustable, it is adjusted for low temperature is unable to, cultivate simple question, now propose following scheme, including chamber door, chamber door side outer wall is hinged with door hinge by the position of two close end, and the side outer wall of door hinge is welded with the same incubator, the chamber door and the opposite side outer wall of incubator are welded with fixed plate by the position of two close end, and the side outer wall of fixed plate has been bolted the same spring.The present invention can be by cell cryopreservation, improve the dormancy time of cell, want to take out at any time when culture and recover, incubation time is shortened, is improved work efficiency, heating or cooling that can be constant to incubator, it survives more suitable for cell proliferation, it to culture dish and effective culture medium can be frozen cleans before and after culture, it is ensured that wash the cell adhered on lower wall, and keep sterile working.
Description
Technical field
The present invention relates to biotechnology technical field more particularly to a kind of cell culture of biotechnology temperature-adjustable
Device.
Background technology
Each subdiscipline of rise with molecular biology and the infiltration to various aspects, bioscience also experiencings the ups and downs
The variation of replacement.From the point of view of current state of development, molecular biology will keep taking the lead the status of subdiscipline, primary study
Field be：The research of the structure and function of large biological molecule；The research of eukaryotic gene and gene expression regulation；Molecule god
Research through biology；The research of Medical Molecular Biology；The research of molecular biology of plants；The research etc. of molecular evolution, it is raw
It needs to cultivate cell, i.e. cell culture apparatus in object technology, along with stem-cell research, recycles medical development, it is desirable that big
The cell of amount modulation clinical application.The modulation of the cell of clinical application requires to meet the operation in the environment of stringent benchmark.
Through retrieval, Chinese patent grant number is the patent of 203187690 U of CN, discloses a kind of cell culture apparatus, is wrapped
Shaking table is included, the shaking table connects retort, and the retort connects outer tank body, the retort and the outer tank body
Between be equipped with heating device, the heating device connect thermostat, the retort connect circulation pipe.It is deposited in above-mentioned patent
In following deficiency：Not having low temperature adjusting, culture is too simple, is easy pollution, and above-mentioned patent cannot solve problems, because
This, there is an urgent need for a kind of cell culture apparatus of biotechnology temperature-adjustable.
Invention content
It is unable to low temperature based on cell culture apparatus to adjust, cultivates simple technical problem, the present invention proposes a kind of biology
The cell culture apparatus of technology temperature-adjustable.
A kind of cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention, including chamber door, the chamber door
Side outer wall is hinged with door hinge by the position of two close end, and the side outer wall of door hinge is welded with the same incubator, institute
It states chamber door and the opposite side outer wall of incubator and is welded with fixed plate by the position of two close end, and the side outer wall of fixed plate is logical
It crosses bolt and is fixed with the same spring, be bolted headlamp in the top inner wall of the incubator, and incubator
It has been bolted temperature sensor in top inner wall, alarm has been bolted in the top exterior walls of the incubator
Lamp, and liquid nitrogen case is welded on the side inner wall of incubator, inlet tube is welded in the top exterior walls of the liquid nitrogen case, and cultivate
Box top outer wall is provided with first through hole close to the position of liquid nitrogen case, and the inlet tube outside first through hole and incubator by being connected
It is logical, it is welded with connecting rod on the side inner wall of the liquid nitrogen case, is provided with and equidistantly distributes on the side outer wall of the connecting rod
First mounting hole, and cryopreservation tube is plugged on the circumferential inner wall of the first mounting hole, it is welded on the side inner wall of the incubator
First partition, and water-bath has been bolted in the top exterior walls of first partition.
Preferably, centrifuge, and the top of first partition have been bolted in the top exterior walls of the first partition
Hydraulic cylinder is bolted on outer wall, one end of hydraulic cylinder extension rod has been bolted culture dish, incubator
Side outer wall be welded with culture medium case, water pump, the water inlet of water pump have been bolted in the bottom outer wall of culture medium case
It is welded with water inlet pipe on inner wall, outlet pipe is welded on the water outlet inner wall of water pump, incubator side outer wall is close to culture medium case
Position be provided with the second through-hole, outlet pipe is connected by the second through-hole with incubator, is bolted on the side outer wall of outlet pipe
The nozzle equidistantly distributed, nozzle face culture dish.
Preferably, second partition is welded on the side inner wall of the incubator, and second partition is located at first partition
Underface is welded with tool box in the bottom outer wall of incubator.
Preferably, the culture bottle equidistantly distributed, and are bolted in the top exterior walls of the second partition
Two partition board top exterior walls are bolted having heaters by the position of two close end.
Preferably, accumulator has been bolted in the bottom interior wall in the tool box, and tool box bottom interior wall is leaned on
The position of nearly accumulator has been bolted processor.
Preferably, the centre position of the tool box bottom interior wall is welded with riser, and tool box bottom interior wall is close to perpendicular
The position of plate has been bolted compressor.
Preferably, the lower surface of the incubator is welded with damper close to four corners, and damper includes pad
The both sides of plate, backing plate bottom outer wall are welded with connecting plate, and automatic telescopic link, automatic telescopic are welded in the bottom outer wall of connecting plate
It is socketed with spring in the outer circumferential walls of bar extension rod, pedestal is welded in the bottom outer wall of automatic telescopic link.
Preferably, operation panel, and the side outer wall of incubator have been bolted on the side outer wall of the chamber door
On be provided with the second mounting hole, be bolted exhaust fan on the side inner wall of the second mounting hole.
Preferably, the temperature sensor is connected by signal wire with the input terminal of processor.
Preferably, the headlamp, centrifuge, alarm lamp, water pump, hydraulic cylinder, accumulator, compressor, heater, row
Gas is fanned and solenoid valve is respectively connected with switch, and switch is connected by signal wire with the output end of processor.
Having the beneficial effect that in the present invention：
1, by liquid nitrogen case, connecting rod and the cryopreservation tube of setting, cell cryopreservation can be improved the dormancy time of cell, be thought
It can take out and recover at any time when cultivating, shorten incubation time, improve work efficiency.
2, by the heater of setting, compressor, temperature sensor and alarm lamp, heating that can be constant to incubator or
Person cools down, and improves the temperature adjustability of device, survives more suitable for cell proliferation.
3, by culture medium case, water pump and the nozzle of setting, to culture dish and effective culture can be frozen before and after culture
Base is cleaned, it is ensured that is washed the cell adhered on lower wall, and is kept sterile working.
4, by the connecting plate of setting, spring, automatic telescopic link and pedestal, the pressure brought when device work has been buffered,
Extend the service life of device.
Description of the drawings
Fig. 1 is a kind of sectional structure signal of cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention
Figure；
Fig. 2 is a kind of main structure diagram of the cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention；
Fig. 3 is a kind of side structure schematic view of the cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention；
Fig. 4 is a kind of liquid nitrogen box structure signal of cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention
Figure；
Fig. 5 is a kind of shock absorber structure signal of cell culture apparatus of biotechnology temperature-adjustable proposed by the present invention
Figure.
In figure：1 chamber door, 2 fixed plates, 3 springs, 4 door hinges, 5 liquid nitrogen casees, 6 incubators, 7 headlamps, 8 centrifuges, 9 reports
Warning lamp, 10 temperature sensors, 11 culture medium casees, 12 nozzles, 13 outlet pipes, 14 water pumps, 15 water inlet pipes, 16 culture dishes, 17 hydraulic pressure
Oil cylinder, 18 first partitions, 19 culture bottles, 20 second partitions, 21 accumulators, 22 processors, 23 backing plates, 24 risers, 25 compressors,
26 heaters, 27 water-baths, 28 operation panels, 29 exhaust fans, 30 solenoid valves, 31 inlet tubes, 32 connecting rods, 33 cryopreservation tubes, 34
Connecting plate, 35 springs, 36 automatic telescopic links, 37 pedestals, 38 tool boxes.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1-5, a kind of cell culture apparatus of biotechnology temperature-adjustable, including chamber door 1,1 side of chamber door
Outer wall is hinged with door hinge 4 by the position of two close end, and the side outer wall of door hinge 4 is welded with the same incubator 6, chamber door
1 and the opposite side outer wall of incubator 6 be welded with fixed plate 2 by the position of two close end, and the side outer wall of fixed plate 2 passes through
Bolt is fixed with the same spring 3, and headlamp 7, and the top of incubator 6 have been bolted in the top inner wall of incubator 6
It has been bolted temperature sensor 10 on portion's inner wall, alarm lamp 9 has been bolted in the top exterior walls of incubator 6,
And liquid nitrogen case 5 is welded on the side inner wall of incubator 6, inlet tube 31, and incubator are welded in the top exterior walls of liquid nitrogen case 5
6 top exterior walls are provided with first through hole close to the position of liquid nitrogen case 5, and inlet tube 31 is connected by first through hole with 6 outside of incubator
It is logical, it is welded with connecting rod 32 on the side inner wall of liquid nitrogen case 5, first equidistantly distributed is provided on the side outer wall of connecting rod 32
Mounting hole, and cryopreservation tube 33 is plugged on the circumferential inner wall of the first mounting hole, cell cryopreservation can be improved the suspend mode of cell
Time, it is desirable to it can take out and recover at any time when cultivating, shorten incubation time, improve work efficiency, the one of incubator 6
It is welded with first partition 18 on the inner wall of side, and has been bolted water-bath 27 in the top exterior walls of first partition 18, uses
When, it is first turned on liquid nitrogen case 5, is taken out cryopreservation tube 33, is then put into 37 DEG C of water-bath 27.
In the present invention, centrifuge 8, and the top of first partition 18 have been bolted in the top exterior walls of first partition 18
Hydraulic cylinder 17 is bolted on portion's outer wall, one end of 17 extension rod of hydraulic cylinder has been bolted culture dish
16, the side outer wall of incubator 6 is welded with culture medium case 11, and water pump has been bolted in the bottom outer wall of culture medium case 11
14, water inlet pipe 15 is welded on the water inlet inner wall of water pump 14, outlet pipe 13 is welded on the water outlet inner wall of water pump 14, is cultivated
6 side outer wall of case is provided with the second through-hole close to the position of culture medium case 11, and outlet pipe 13 is connected by the second through-hole with incubator 6
It is logical, the nozzle 12 equidistantly distributed is bolted on the side outer wall of outlet pipe 13, nozzle 12 faces culture dish 16, waits for that liquid melts
After change, takes out and cell suspension is drawn onto in centrifuge tube, be then placed in centrifuge 8, after the completion of centrifugation, outwell supernatant, add
Cell is suspended by 1ml culture mediums, is then started water pump 14 and is cleaned culture dish 16 using nozzle 12, is then drawn onto cell
It gently shakes, is cultivated in culture dish 16, to culture dish and effective culture medium can be frozen clean before and after culture, really
Guarantor washes the cell adhered on lower wall, and keeps sterile working.
Be welded with second partition 20 on the side inner wall of incubator 6, and second partition 20 be located at first partition 18 just under
It is square, it is welded with tool box 38 in the bottom outer wall of incubator 6, has been bolted in the top exterior walls of second partition 20 equidistant
Culture bottle 19 from distribution, and 20 top exterior walls of second partition are bolted having heaters 26, work by the position of two close end
It is bolted accumulator 21 in the bottom interior wall for having case 38, and 38 bottom interior wall of tool box is close to the position of accumulator 21
It is bolted processor 22, the centre position of 38 bottom interior wall of tool box is welded with riser 24, and 38 bottom of tool box
Inner wall has been bolted compressor 25 close to the position of riser 24, and heating or cooling that can be constant to incubator carry
The high temperature adjustability of device, survives more suitable for cell proliferation.
The lower surface of incubator 6 is welded with damper close to four corners, and damper includes backing plate 23, backing plate 23
The both sides of bottom outer wall are welded with connecting plate 34, and automatic telescopic link 36, automatic telescopic are welded in the bottom outer wall of connecting plate 34
It is socketed with spring 35 in the outer circumferential walls of 36 extension rod of bar, pedestal 37, chamber door 1 are welded in the bottom outer wall of automatic telescopic link 36
Side outer wall on be bolted operation panel 28, and the second mounting hole is provided on the side outer wall of incubator 6, second
It has been bolted exhaust fan 29 on the side inner wall of mounting hole, then can select to be put into culture bottle 19 and carry out continuing to train
It supports, compressor 15 and heater 26 can carry out temperature adjustment to 6 inside of incubator, and temperature sensor 10 monitors the temperature inside the box, temperature
Degree sensor 10 is connected by signal wire with the input terminal of processor 22, headlamp 7, centrifuge 8, alarm lamp 9, water pump 14, liquid
Compressing cylinder 17, accumulator 21, compressor 25, heater 26, exhaust fan 29 and solenoid valve 30 are respectively connected with switch, and switch passes through
Signal wire is connected with the output end of processor 22, the model ARM10TDMI of processor 22.
In use, being first turned on liquid nitrogen case 5, it is taken out cryopreservation tube 33, then puts into 37 DEG C of water-bath 27, waits for liquid
After body melts, takes out and cell suspension is drawn onto in centrifuge tube, be then placed in centrifuge 8, after the completion of centrifugation, outwell supernatant
Liquid adds 1ml culture mediums that cell suspends, and then starts water pump 14 and is cleaned culture dish 16 using nozzle 12, then will be thin
Born of the same parents are drawn onto in culture dish 16 and gently shake, and are cultivated, and then can select to be put into culture bottle 19 and carry out continuing to cultivate, and compress
Machine 15 and heater 26 can carry out temperature adjustment to 6 inside of incubator, and temperature sensor 10 monitors the temperature inside the box.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of cell culture apparatus of biotechnology temperature-adjustable, including chamber door（1）, which is characterized in that the chamber door
（1）Side outer wall is hinged with door hinge by the position of two close end（4）, and door hinge（4）Side outer wall be welded with it is same
Incubator（6）, the chamber door（1）And incubator（6）Opposite side outer wall is welded with fixed plate by the position of two close end
（2）, and fixed plate（2）Side outer wall be bolted the same spring（3）, the incubator（6）Top inner wall
On be bolted headlamp（7）, and incubator（6）Top inner wall on be bolted temperature sensor
（10）, the incubator（6）Top exterior walls on be bolted alarm lamp（9）, and incubator（6）Side inner wall on
It is welded with liquid nitrogen case（5）, the liquid nitrogen case（5）Top exterior walls on be welded with inlet tube（31）, and incubator（6）Top exterior walls
Close to liquid nitrogen case（5）Position be provided with first through hole, the inlet tube（31）Pass through first through hole and incubator（6）Outside is connected
It is logical, the liquid nitrogen case（5）Side inner wall on be welded with connecting rod（32）, the connecting rod（32）Side outer wall on be provided with
First mounting hole of range distribution, and it is plugged with cryopreservation tube on the circumferential inner wall of the first mounting hole（33）, the incubator（6）'s
It is welded with first partition on the inner wall of side（18）, and first partition（18）Top exterior walls on be bolted water-bath
（27）.
2. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that institute
State first partition（18）Top exterior walls on be bolted centrifuge（8）, and first partition（18）Top exterior walls on
It has been bolted hydraulic cylinder（17）, hydraulic cylinder（17）One end of extension rod has been bolted culture dish（16）,
Incubator（6）Side outer wall be welded with culture medium case（11）, culture medium case（11）Bottom outer wall on be bolted
Water pump（14）, water pump（14）Water inlet inner wall on be welded with water inlet pipe（15）, water pump（14）Water outlet inner wall on be welded with
Outlet pipe（13）, incubator（6）Side outer wall is close to culture medium case（11）Position be provided with the second through-hole, outlet pipe（13）Pass through
Second through-hole and incubator（6）It is connected, outlet pipe（13）Side outer wall on be bolted with the nozzle equidistantly distributed（12）, spray
Head（12）Face culture dish（16）.
3. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that institute
State incubator（6）Side inner wall on be welded with second partition（20）, and second partition（20）Positioned at first partition（18）Just
Lower section, incubator（6）Bottom outer wall on be welded with tool box（38）.
4. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 3, which is characterized in that institute
State second partition（20）Top exterior walls on be bolted the culture bottle equidistantly distributed（19）, and second partition（20）
Top exterior walls are bolted having heaters by the position of two close end（26）.
5. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 3, which is characterized in that institute
State tool box（38）Bottom interior wall on be bolted accumulator（21）, and tool box（38）Bottom interior wall is close to electric power storage
Pond（21）Position be bolted processor（22）.
6. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 3, which is characterized in that institute
State tool box（38）The centre position of bottom interior wall is welded with riser（24）, and tool box（38）Bottom interior wall is close to riser（24）
Position be bolted compressor（25）.
7. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that institute
State incubator（6）Lower surface be welded with damper close to four corners, and damper includes backing plate（23）, backing plate（23）
The both sides of bottom outer wall are welded with connecting plate（34）, connecting plate（34）Bottom outer wall on be welded with automatic telescopic link（36）, from
Dynamic telescopic rod（36）It is socketed with spring in the outer circumferential walls of extension rod（35）, automatic telescopic link（36）Bottom outer wall on be welded with
Pedestal（37）.
8. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that institute
State chamber door（1）Side outer wall on be bolted operation panel（28）, and incubator（6）Side outer wall on be provided with
Two mounting holes have been bolted exhaust fan on the side inner wall of the second mounting hole（29）.
9. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that institute
State temperature sensor（10）Pass through signal wire and processor（22）Input terminal be connected.
10. a kind of cell culture apparatus of biotechnology temperature-adjustable according to claim 1, which is characterized in that
The headlamp（7）, centrifuge（8）, alarm lamp（9）, water pump（14）, hydraulic cylinder（17）, accumulator（21）, compressor
（25）, heater（26）, exhaust fan（29）And solenoid valve（30）It is respectively connected with switch, and switchs and passes through signal wire and processor
（22）Output end be connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810706996.6A CN108795745A (en) | 2018-07-02 | 2018-07-02 | A kind of cell culture apparatus of biotechnology temperature-adjustable |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810706996.6A CN108795745A (en) | 2018-07-02 | 2018-07-02 | A kind of cell culture apparatus of biotechnology temperature-adjustable |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108795745A true CN108795745A (en) | 2018-11-13 |
Family
ID=64074021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810706996.6A Withdrawn CN108795745A (en) | 2018-07-02 | 2018-07-02 | A kind of cell culture apparatus of biotechnology temperature-adjustable |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108795745A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111334411A (en) * | 2020-03-02 | 2020-06-26 | 内蒙古自治区生物技术研究院 | A shock attenuation shaking table for growth of astragalus root hairy root |
| CN112980685A (en) * | 2021-04-20 | 2021-06-18 | 广州赛太特生物医学科技有限公司 | A biological cell breeding device for cultivateing, survey |
| CN113150987A (en) * | 2021-04-21 | 2021-07-23 | 浙江大学 | Cell biology experiment sample culture equipment |
-
2018
- 2018-07-02 CN CN201810706996.6A patent/CN108795745A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111334411A (en) * | 2020-03-02 | 2020-06-26 | 内蒙古自治区生物技术研究院 | A shock attenuation shaking table for growth of astragalus root hairy root |
| CN112980685A (en) * | 2021-04-20 | 2021-06-18 | 广州赛太特生物医学科技有限公司 | A biological cell breeding device for cultivateing, survey |
| CN113150987A (en) * | 2021-04-21 | 2021-07-23 | 浙江大学 | Cell biology experiment sample culture equipment |
| CN113150987B (en) * | 2021-04-21 | 2023-12-05 | 浙江大学 | Cell biology experiment sample culture equipment |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181113 |
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| WW01 | Invention patent application withdrawn after publication |