CN111334411A - A shock attenuation shaking table for growth of astragalus root hairy root - Google Patents

A shock attenuation shaking table for growth of astragalus root hairy root Download PDF

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Publication number
CN111334411A
CN111334411A CN202010134764.5A CN202010134764A CN111334411A CN 111334411 A CN111334411 A CN 111334411A CN 202010134764 A CN202010134764 A CN 202010134764A CN 111334411 A CN111334411 A CN 111334411A
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China
Prior art keywords
shaking
shaking table
bottom plate
plate
motor
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CN202010134764.5A
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Chinese (zh)
Inventor
张秀娟
白朕卿
随洋
田雨
吴佳文
高新新
陈启渊
张宇森
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Inner Mongolia Autonomous Region Institute Of Biotechnology
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Inner Mongolia Autonomous Region Institute Of Biotechnology
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Priority to CN202010134764.5A priority Critical patent/CN111334411A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a shock-absorbing shaking table for growth of astragalus membranaceus hairy roots, which comprises a bottom plate, a shaking plate and a mounting seat, wherein a control chamber is welded on one side of the upper surface of the bottom plate, a culture chamber is welded on the other side of the upper surface of the bottom plate, a supporting seat is welded on the lower surface of the bottom plate, a cavity is formed in the bottom end of the culture chamber, a motor is fixed in the cavity, an eccentric turntable is mounted on an output main shaft of the motor and is positioned at the bottom end of the inner wall of the culture chamber, an eccentric shaft is fixed on the eccentric turntable, a connecting rod is movably inserted into the lower surface of the shaking plate, a mounting block is rotatably mounted at the bottom end of the. The vibration reduction table is compact in structure and convenient and fast to use, and the vibration reduction structure is additionally arranged on the basis of the traditional table concentrator, so that the working noise of the table concentrator is reduced, and the use requirements of personnel are met.

Description

A shock attenuation shaking table for growth of astragalus root hairy root
Technical Field
The invention relates to the technical field of astragalus membranaceus hairy root culture, in particular to a vibration-damping shaking table for growth of astragalus membranaceus hairy roots.
Background
The hairy root culture is a technique combining gene engineering and cell engineering, by integrating T-DNA contained in Ri plasmid in agrobacterium rhizogenes on DNA of plant cells to induce the plant cells to generate hairy roots, the hairy roots belong to hormone autotrophic type, have the characteristics of high content of effective components, stable physiological biochemistry and heredity, easy operation control and the like, can show the capability of synthesizing secondary metabolites under the condition of in vitro culture, can also synthesize a plurality of substances which can not be synthesized by suspension cell culture, the yield of certain products is even higher than that of normal plants and suspension cell culture, therefore, the hairy root culture system shows unique superiority in the aspects of biomass increase, medicinal effective component accumulation and production stability, so that the production of plant secondary metabolites by hairy root culture has great production potential.
The hairy roots of medicinal plants such as henbane, astragalus, lithospermum, safflower, sweet wormwood, cassia tora and the like are successfully cultured, and the ginsenoside, the berberine and the like are industrially produced by culturing the hairy roots. How to realize the large-scale culture of the hairy roots of the medicinal plants and improve the accumulation capacity of effective secondary metabolites in the hairy roots become the focus of attention of many researchers.
Generally, a shaking table required in the process of culturing the astragalus hairy roots is not provided with a damping structure, so that the dead weight of a culture medium is larger, the working noise is larger, the culture of the hairy roots is not facilitated, and the use requirement of personnel cannot be met.
Disclosure of Invention
The invention aims to provide a vibration-damping shaking table for astragalus membranaceus hairy root growth, which has the advantages of being compact in structure and convenient to use, reducing the working noise of the shaking table by additionally arranging a vibration-damping structure on the basis of a traditional shaking table, meeting the use requirements of personnel, and solving the problems that the shaking table required in the general astragalus membranaceus hairy root culture process is not provided with a vibration-damping structure, the dead weight of a culture medium is larger, the working noise of the shaking table is larger, the culture of the hairy roots is not facilitated, and the use requirements of the personnel cannot be met.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a shock attenuation shaking table for growth of astragalus root hairy root, includes the bottom plate, shakes movable plate and mount pad, the welding of upper surface one side of bottom plate has the control room, and the welding of the upper surface opposite side of bottom plate has the room of cultivateing, and the lower skin weld of bottom plate has the supporting seat, cultivate the bottom of room and seted up the cavity, the cavity internal fixation has the motor, and installs eccentric carousel on the output main shaft of motor, and eccentric carousel is located cultivates the inner wall bottom of room, and is fixed with the eccentric shaft on the eccentric carousel, the lower surface activity of shaking the movable plate is pegged graft and is had the connecting rod, and the bottom of connecting rod is rotated and is installed.
Preferably, a sealing bearing is installed at the connecting position of the output main shaft of the motor and the culture chamber, a heat dissipation window is arranged at the lower end of the outer wall of the culture chamber, and the position of the heat dissipation window corresponds to the position of the motor.
Preferably, the upper surface welding of mount pad has the gag lever post, and the top slidable mounting of gag lever post is in shaking the lower surface of board, and the lower surface of mount pad rotates and installs the roller pearl.
Preferably, the limiting rod is movably sleeved with a spring, the spring is located between the lower surface of the shaking plate and the upper surface of the mounting base, and the original length of the spring is greater than the length of the connecting rod.
Preferably, the culture chamber is rotatably provided with a movable door through a hinged chain, and the movable door is provided with a door handle in a threaded manner.
Preferably, the number of the supporting seats is four, and the four supporting seats are distributed on the lower surface of the bottom plate in a rectangular array.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention relates to a shock-absorbing shaking table for the growth of astragalus root hairy roots, which can place materials to be cultured in a substrate in a flask, then place the flask on a shaking plate, the surface of the shaking plate can adopt a frosted rough design to increase the friction force between the shaking plate and the flask, then control the work of a motor through an external power supply, a driving shaft of the motor drives an eccentric turntable to rotate so as to drive an eccentric shaft to rotate, the shaking plate is driven by a connecting rod to generate shaking similar to the circumference under the eccentric operation of the eccentric shaft, roll beads designed at four corners of the lower surface of the shaking plate can provide supporting force for the shaking plate while the shaking plate shakes so as to ensure the moving balance of the shaking plate, when needed, a tool can rotate an installation block, and the installation block is separated from the eccentric shaft through the thread meshing effect between the installation block and the eccentric shaft so as to detach the shaking plate, is convenient to clean.
2. When the shock absorption shaking table for the growth of the astragalus membranaceus hairy roots is used, through the design of the springs, when a heavy object is placed on the shaking plate, the shaking plate moves downwards under the action of gravity, and then the connecting rod and the limiting rod are driven to retract into the shaking plate, at the moment, the springs are extruded by the shaking plate to deform to generate elasticity, an effective shock absorption effect can be achieved under the action of the elasticity of the springs, the shock absorption structure is additionally arranged on the basis of the traditional shaking table, the working noise of the shaking table is reduced, and the use requirements of personnel are met.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic view of the external structure of the present invention;
FIG. 3 is a schematic top view of the culture chamber of the present invention;
FIG. 4 is a schematic diagram of the structure A of FIG. 1 according to the present invention;
FIG. 5 is a schematic structural diagram of B in FIG. 1 according to the present invention.
In the figure: 1. a base plate; 2. a control room; 3. a culture chamber; 4. a cavity; 5. a motor; 6. a supporting seat; 7. an eccentric turntable; 8. a shaking plate; 9. an eccentric shaft; 10. mounting blocks; 11. a connecting rod; 12. a limiting rod; 13. a spring; 14. a mounting seat; 15. rolling beads; 16. a movable door; 17. a door handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 5, an embodiment of the present invention includes: the utility model provides a shock attenuation shaking table for growth of hairy root of radix astragali, includes bottom plate 1, shake movable plate 8 and mount pad 14, and the welding of upper surface one side of bottom plate 1 has control room 2, and the welding of the upper surface opposite side of bottom plate 1 has cultivates room 3, and the lower surface welding of bottom plate 1 has supporting seat 6, and supporting seat 6 is totally four, and four supporting seats 6 are rectangular array and distribute at the lower surface of bottom plate 1, cultivates and install dodge gate 16 through articulated chain rotation on the room 3, and the door 17 is installed to the screw thread on the dodge gate 16.
In the using process, the material to be cultured can be placed in the matrix in the flask, then the flask is placed on the shaking plate 8, the surface of the shaking plate 8 can adopt a frosted rough design, and the friction force between the shaking plate and the flask is increased.
Cultivate the bottom of room 3 and seted up cavity 4, cavity 4 internal fixation has motor 5, and install eccentric carousel 7 on motor 5's the output main shaft, eccentric carousel 7 is located cultivates the inner wall bottom of room 3, and be fixed with eccentric shaft 9 on the eccentric carousel 7, it has connecting rod 11 to rock the activity of the lower surface of board 8 and peg graft, the bottom of connecting rod 11 is rotated and is installed installation piece 10, installation piece 10 screw thread is installed on eccentric shaft 9, through external power supply control motor 5 work, motor 5's driving shaft drives eccentric carousel 7 and rotates, and then drive eccentric shaft 9 and rotate, it shakes the rocking plate 8 production of being similar to the circumference to shake through connecting rod 11 drive under eccentric operation of eccentric shaft 9.
The motor 5 is BDY-200B in model number, and has the advantages of small size, low energy consumption, low noise and the like.
A sealing bearing is arranged at the connecting position of an output main shaft of the motor 5 and the culture chamber 3, a heat radiation window is arranged at the lower end of the outer wall of the culture chamber 3, and the position of the heat radiation window corresponds to the position of the motor 5.
Through shaking the roller 15 of 8 lower surface four corners designs of movable plate, can be when shaking plate 8 and rock and provide the holding power for shaking plate 8, ensure to shake movable plate 8's removal balance, when needs, the accessible instrument rotates installation piece 10, thereby makes installation piece 10 break away from eccentric shaft 9 through the thread engagement effect between installation piece 10 and the eccentric shaft 9 and will shake movable plate 8 and pull down, be convenient for clear up it.
The upper surface of the mounting seat 14 is welded with a limiting rod 12, the top end of the limiting rod 12 is slidably mounted on the lower surface of the shaking plate 8, and the lower surface of the mounting seat 14 is rotatably provided with a roller ball 15, the limiting rod 12 is movably sleeved with a spring 13, the spring 13 is positioned between the lower surface of the shaking plate 8 and the upper surface of the mounting seat 14, the original length of the spring 13 is greater than that of the connecting rod 11, through the design of the spring 13, when a heavy object is placed on the shaking plate 8, the shaking plate 8 moves downwards under the pressing action of gravity, and then the connecting rod 11 and the limiting rod 12 are both driven to retract into the shaking plate 8, at this time, the spring 13 is extruded by the shaking plate 8 to generate deformation and further generate elasticity, can play effectual damping effect under spring 13's elasticity effect, add the damping structure on the basis of traditional shaking table, reduce the noise at work of shaking table, satisfy personnel's user demand.
The working principle is as follows: the shock absorption shaking table for the growth of the astragalus mongholicus hairy roots can place materials to be cultured in a substrate in a flask in the using process, then the flask is placed on a shaking plate 8, the surface of the shaking plate 8 can adopt a frosted rough design to increase the friction force between the shaking plate and the flask, then the motor 5 is controlled to work by an external power supply, a driving shaft of the motor 5 drives the eccentric turntable 7 to rotate, further drives the eccentric shaft 9 to rotate, under the eccentric operation of the eccentric shaft 9, the connecting rod 11 drives the shaking plate 8 to shake similar to the circumference, and through the roller beads 15 arranged at four corners of the lower surface of the shaking plate 8, can provide supporting force for the shaking plate 8 when the shaking plate 8 shakes, ensures the movement balance of the shaking plate 8, when necessary, the mounting block 10 is turned by a tool, and the shaking plate 8 is removed by disengaging the mounting block 10 from the eccentric shaft 9 through the screw engagement between the mounting block 10 and the eccentric shaft 9.
Through the design of spring 13, when the heavy object was placed on rocking plate 8, rocking plate 8 moved down under the oppression effect of gravity, and then drove connecting rod 11 and gag lever post 12 and all retracted in rocking plate 8, at this moment, thereby spring 13 receives the extrusion of rocking plate 8 and produces deformation and then produce elasticity, can play effectual damping effect under spring 13's elastic force effect.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. The utility model provides a shock attenuation shaking table for growth of astragalus hairy root, includes bottom plate (1), rocks board (8) and mount pad (14), its characterized in that: the welding of upper surface one side of bottom plate (1) has control room (2), and the welding of the upper surface opposite side of bottom plate (1) has cultivation room (3), and the lower surface welding of bottom plate (1) has supporting seat (6), cultivate the bottom of room (3) and seted up cavity (4), cavity (4) internal fixation has motor (5), and installs eccentric carousel (7) on the output main shaft of motor (5), and eccentric carousel (7) are located the inner wall bottom of cultivating room (3), and are fixed with eccentric shaft (9) on eccentric carousel (7), the lower surface activity of shaking movable plate (8) is pegged graft and is had connecting rod (11), and the bottom of connecting rod (11) is rotated and is installed installation piece (10), and installation piece (10) screw thread is installed on eccentric shaft (9).
2. The vibration-damping shaking table for astragalus mongholicus hairy root growth according to claim 1, which is characterized in that: a sealing bearing is installed at the connecting position of an output main shaft of the motor (5) and the culture chamber (3), a heat dissipation window is arranged at the lower end of the outer wall of the culture chamber (3), and the position of the heat dissipation window corresponds to that of the motor (5).
3. The vibration-damping shaking table for astragalus mongholicus hairy root growth according to claim 1, which is characterized in that: the upper surface welding of mount pad (14) has gag lever post (12), and the top slidable mounting of gag lever post (12) is at the lower surface of shaking board (8), and the lower surface of mount pad (14) rotates and installs roller pearl (15).
4. The vibration-damping shaking table for astragalus mongholicus hairy root growth according to claim 1, which is characterized in that: the spring (13) is movably sleeved on the limiting rod (12), the spring (13) is located between the lower surface of the shaking plate (8) and the upper surface of the mounting base (14), and the original length of the spring (13) is larger than that of the connecting rod (11).
5. The vibration-damping shaking table for astragalus mongholicus hairy root growth according to claim 1, which is characterized in that: the culture room (3) is rotatably provided with a movable door (16) through a hinged chain, and the movable door (16) is provided with a door handle (17) through threads.
6. The vibration-damping shaking table for astragalus mongholicus hairy root growth according to claim 1, which is characterized in that: the number of the supporting seats (6) is four, and the four supporting seats (6) are distributed on the lower surface of the bottom plate (1) in a rectangular array.
CN202010134764.5A 2020-03-02 2020-03-02 A shock attenuation shaking table for growth of astragalus root hairy root Pending CN111334411A (en)

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CN202010134764.5A CN111334411A (en) 2020-03-02 2020-03-02 A shock attenuation shaking table for growth of astragalus root hairy root

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CN202010134764.5A CN111334411A (en) 2020-03-02 2020-03-02 A shock attenuation shaking table for growth of astragalus root hairy root

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103357326A (en) * 2012-04-08 2013-10-23 昆山市锦鑫生物科技有限公司 Rocking bed
CN207357055U (en) * 2017-10-08 2018-05-15 徐志华 A kind of double drive coating agitator tanks
CN207641392U (en) * 2017-11-28 2018-07-24 天津慧智同科技有限责任公司 A kind of reciprocal concussion water bath with thermostatic control shaking table
CN108795745A (en) * 2018-07-02 2018-11-13 郑州丽福爱生物技术有限公司 A kind of cell culture apparatus of biotechnology temperature-adjustable
CN109055214A (en) * 2018-08-20 2018-12-21 唐燕 A kind of cell incubator of the transfer easy to carry to genetic engineering
CN208791654U (en) * 2018-07-12 2019-04-26 湖南未名三胞转化医学科技有限公司 Cell culture apparatus is used in a kind of detection of stem cell
CN209260100U (en) * 2018-12-13 2019-08-16 田娟 A kind of pair of damaged cell has the human stem cell culture instrument of On Reactivation
CN209412230U (en) * 2018-11-06 2019-09-20 广州百惟宁生物科技有限公司 A kind of biological reagent cell incubator

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103357326A (en) * 2012-04-08 2013-10-23 昆山市锦鑫生物科技有限公司 Rocking bed
CN207357055U (en) * 2017-10-08 2018-05-15 徐志华 A kind of double drive coating agitator tanks
CN207641392U (en) * 2017-11-28 2018-07-24 天津慧智同科技有限责任公司 A kind of reciprocal concussion water bath with thermostatic control shaking table
CN108795745A (en) * 2018-07-02 2018-11-13 郑州丽福爱生物技术有限公司 A kind of cell culture apparatus of biotechnology temperature-adjustable
CN208791654U (en) * 2018-07-12 2019-04-26 湖南未名三胞转化医学科技有限公司 Cell culture apparatus is used in a kind of detection of stem cell
CN109055214A (en) * 2018-08-20 2018-12-21 唐燕 A kind of cell incubator of the transfer easy to carry to genetic engineering
CN209412230U (en) * 2018-11-06 2019-09-20 广州百惟宁生物科技有限公司 A kind of biological reagent cell incubator
CN209260100U (en) * 2018-12-13 2019-08-16 田娟 A kind of pair of damaged cell has the human stem cell culture instrument of On Reactivation

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Application publication date: 20200626