CN112980685A

CN112980685A - A biological cell breeding device for cultivateing, survey

Info

Publication number: CN112980685A
Application number: CN202110421597.7A
Authority: CN
Inventors: 赖振祥; 林楚玲
Original assignee: Cytate Biomedical(guangzhou) Technologies Inc
Current assignee: Cytate Biomedical(guangzhou) Technologies Inc
Priority date: 2021-04-20
Filing date: 2021-04-20
Publication date: 2021-06-18
Anticipated expiration: 2041-04-20
Also published as: CN112980685B

Abstract

The invention discloses a biological cell cultivating device for cultivation and determination, which comprises a box body, wherein a ventilation mechanism and a water replenishing tank are arranged in the box body, the ventilation mechanism is connected with a water replenishing tank pipeline, the water replenishing tank is connected with a box body pipeline, the ventilation mechanism carries out sterilization and dust removal treatment on air, and the water replenishing tank replenishes moisture to the air. Mechanism takes a breath successively heats and cools down the processing through the peltier effect to the air, make moisture in the air condense and with air separation, mechanism takes a breath adsorbs the dust in the air through the water droplet that cools down the condensation, thereby reach air-purifying's effect, and dry the air, thereby the convenience carries out accurate control to the humidity of air, the moisturizing case supplements moisture to dry air, the moisturizing case controls the moisture that contains in the air, thereby reach accurate control air humidity's purpose, and then reach the accurate control of biological cell growing environment.

Description

A biological cell breeding device for cultivateing, survey

Technical Field

The invention relates to the technical field of biological cell culture, in particular to a biological cell culture device for culture and determination.

Background

With the rapid development of the field of regenerative medicine, incubators are widely used to culture cells by adjusting the temperature and humidity in the incubator or adjusting the carbon dioxide concentration, nitrogen concentration, etc. as needed. The key to the success of biological cell culture lies in aseptic operation and temperature and humidity control, and it is important to ensure a clean, independent, constant-temperature and moderate-humidity environment.

Most simple structure of present biological cell culture device needs the manual work cooperation operation, and the inside temperature difference everywhere of incubator is bigger moreover, and the control of temperature is difficult for holding to the cultivation of microorganism has been influenced.

Meanwhile, in order to adjust the air humidity in the incubator, it is common to provide a humidifying plate in the incubator, store water therein, and adjust the humidity by natural evaporation of the stored water. However, the humidity of the natural evaporation is not easily controlled and not evenly distributed in the incubator. Therefore, a biological cell culturing apparatus for culturing and measuring is required to solve the above problems.

Disclosure of Invention

The present invention is directed to a biological cell culture apparatus for culturing and measuring, which solves the above-mentioned problems of the background art.

In order to solve the technical problems, the invention provides the following technical scheme: a biological cell culture device for culture and determination comprises a box body, wherein an air exchange mechanism and a water replenishing box are arranged in the box body, the air exchange mechanism is connected with a water replenishing box pipeline, the water replenishing box is connected with a box body pipeline, the air exchange mechanism carries out sterilization and dust removal treatment on air, and the water replenishing box replenishes moisture to the air; mechanism takes a breath successively heats and cools down the processing through the peltier effect to the air, make moisture in the air condense and with air separation, mechanism takes a breath adsorbs the dust in the air through the water droplet that cools down the condensation, thereby reach air-purifying's effect, and dry the air, thereby the convenience carries out accurate control to the humidity of air, the moisturizing case supplements moisture to dry air, the moisturizing case controls the moisture that contains in the air, thereby reach accurate control air humidity's purpose, and then reach the accurate control of biological cell growing environment.

A bearing plate is arranged in the box body, an incubator is arranged on the bearing plate, an air inlet pipe is arranged at one end of the bearing plate, and one end of the air inlet pipe is positioned above the incubator; the incubator is used for bearing the culture dish of cultivateing biological cell, and the loading board provides the support to placing of incubator, and the intake pipe is transmitted the incubator top with the air that flows out in the moisturizing case, makes the air that has moisture from the culture dish top toward moving.

The air exchange mechanism comprises two groups of fans, two groups of air inlet covers, two groups of sterilization polarization boxes and an impurity removal box, wherein each group of fans is fixed with the box body; the fan extracts external air and infuses sterilization polarization case through the air inlet cover, sterilization polarization case sterilizes the bacterium in the air through the electric field, simultaneously polarize the dust in the air through the electric field, sterilization polarization case heats the air, make the air inflation, thereby make the distance between bacterium and the dust enlarge, make the bacterium expose in the air, the bacterium is when through the electric field, the electric current that makes between the electric field flow kills, the edulcoration case is cooled down to the hot-air, make moisture in the air meet the condensation knot, make the dust in the air, the bacterium fuses with the water droplet that condenses, thereby the realization is to the purification and the dehydration drying of air, the dry air that flows out in the edulcoration case enters into the moisturizing case and replenishes moisture, carry out dehydration with the air, convenient follow-up accurate control to air humidity.

The inside both sides of moisturizing case all are provided with the fixed plate, every group all be provided with the fly leaf on the fixed plate, it is a set of the fly leaf upper end is provided with the drainage plate, drainage plate below fly leaf, top be provided with a set of drainage plate fixed plate and four mutually support of moisturizing case are at the inside formation air flow space of moisturizing case, edulcoration case and air flow space intercommunication, the one end and the moisturizing case sliding connection of drainage plate are provided with the sealing layer between the other end of drainage plate and another group's fly leaf, be provided with the outlet duct on the sealing layer, outlet duct and intake pipe piping connection. The fixed plate mutually supports with the fly leaf and forms the play water space that follows the water level decline and reduce, the drainage carries out the drainage to dry air, the drainage board, two sets of fly leaves, two sets of fixed plates and sealing layer mutually support and form the moisturizing space, the sealing layer highly controls the flow of air, the drainage board is in the moisturizing space with air drainage from the air flow space, the sealing layer makes the air can only flow on the surface of water, the air that flows at a high speed makes the hydrone on surface shake off the constraint of other molecules and enter into the air, and the air that flows is hot-air, further the separation from of acceleration hydrone and enter into in the air, thereby realize the moisturizing effect to the air, the outlet duct provides the passageway for the outflow of moisturizing air, make the air behind the moisturizing enter into in the intake pipe.

Be provided with solid fixed ring in the sterilization polarization case, gu fixed ring top is provided with the sterilization polarization board, the middle part position of sterilization polarization board is provided with a set of vertical breather pipe, and the outside that lies in the middle part breather pipe on the sterilization polarization board is provided with other curved breather pipe that is of a plurality of groups, every group the outside of breather pipe is provided with a plurality of groups heating block, every group all be provided with the first semiconductor of two kinds of differences on the heating block, a plurality of groups the other end and the edulcoration case of first semiconductor are connected. The fixing ring supports the installation of the sterilization polarization plate, the sterilization polarization plate blocks the air flowing space in the sterilization polarization box, meanwhile, the air pipe supports the installation of the air pipe, the air pipe supports the flowing of the air, the arc-shaped pipeline prolongs the flowing time of the air in the pipeline, the times of mutual collision of dust in the air in the pipeline are increased, the heating block is a hot end in the Peltier effect, a plurality of groups of first semiconductors are equally divided into P-type semiconductors and N-type semiconductors, the current flows in the first semiconductors, the heating block is enabled to heat the air pipe, and therefore the air pipe is heated and sterilized.

The sterilization device is characterized in that a plurality of groups of electric field shafts are arranged in the ventilation pipe, two ends of each group of electric field shafts are fixed with the sterilization polarization plate, a plurality of groups of heating blocks are arranged in the ventilation pipe, two different second semiconductors are arranged on each group of heating blocks, the groups of heating blocks are connected in parallel through the second semiconductors, and the groups of second semiconductors are connected with the groups of electric field shafts. The plurality of groups of second semiconductors are also equally divided into P-type semiconductors and N-type semiconductors, the heating block is a metal plate which heats the two groups of semiconductors in the Seebeck effect, the heating plate enables electrons in the second semiconductors to flow under the heating of the vent pipe, the plurality of groups of electric field shafts are installed in the vent pipe, two ends of each electric field shaft are installed on the sterilization polarization plate, the plurality of groups of electric field shafts are divided into positive electric field shafts and negative electric field shafts, the plurality of groups of electric field shafts are electrically connected with the plurality of groups of second semiconductors, positive electrons and negative electrons are respectively distributed on the plurality of groups of electric field shafts, so that an electric field is generated between the plurality of groups of electric field shafts, the electric field polarizes dust in the air, and meanwhile, the electric field kills bacteria through the.

The upper end of each group of the sterilization polarization boxes is provided with a conveying pipe, each group of the conveying pipes comprises a polymerization pipe, the other end of the polymerization pipe is communicated with the impurity removal box, a plurality of groups of furling rings are arranged inside the polymerization pipe, and an expansion groove is formed between every two groups of furling rings inside the polymerization pipe. Draw in the ring and make the inside air flow space of polymerization pipe reduce, make the dust mutually collide when drawing in the ring in to the dust that makes after the polarization adsorbs each other and forms big dust particle, takes place the adhesion with the bacterium after killing when bumping simultaneously, and the expansion tank makes the pipe wall of polymerization pipe change, thereby destroys the laminar flow of air in the polymerization pipe, makes the air current disorder, thereby further improves the absorption between the dust, thereby improves dust removal effect.

The inside curved backup pad that is provided with of edulcoration incasement portion, the top of backup pad is provided with the edulcoration board that is the inversion of V type, it is provided with two sets of refrigeration boards, two sets of to remove on the miscellaneous board refrigeration board and the other end electric connection of the first semiconductor of a plurality of groups, one of them first semiconductor and power electric connection, the edulcoration case is gone up and is corresponded the position that removes miscellaneous board and seted up the outlet, and the edulcoration case is located the below of outlet and has seted up the gas vent, and the edulcoration case outside corresponds the position of outlet and is provided with the drain pipe, and the edulcoration case outside corresponds the position of gas vent and is provided with the gas-supply pipe, gas-supply pipe and moisturizing case pipe connection, a plurality of groups of air vents have been seted up in the backup. The support plate provides support for the installation of the impurity removing plate, the support plate and the impurity removing plate are matched with each other to form a flowing space of wastewater in the impurity removing box, the refrigeration plate is arranged on the inner side of the impurity removing plate, the refrigeration plate is a cold end in the Peltier effect, the refrigeration plate cools the impurity removing plate, hot air flows out of the polymerization pipe and is cooled in the impurity removing box and is in contact with the low-temperature impurity removing plate, so that water drops are formed on the surface of the impurity removing plate, meanwhile, dust particles, bacteria and the like in the air are attached to the impurity removing plate, the water drops are continuously formed along with the continuous entering of the hot air and slide down on the impurity removing plate, the water drops flow to the concave part of the support plate through the water through holes and flow into the water discharging pipe through the water discharging port, the water drops wash the attached dust, dust particles and bacteria when sliding on the impurity removing plate, the cleaning of the impurity removing plate is realized, and the partition plate separates, and the purified dry air enters the water replenishing box through the vent hole and the exhaust port.

The temperature control device comprises a gas conveying pipe, and is characterized in that a temperature control ring is arranged on the gas conveying pipe, an inner ring plate and an outer ring plate are arranged inside the temperature control ring from inside to outside, the temperature control ring and the outer ring plate are matched with each other to form a ventilation space, the pipe diameter of the inner ring plate is the same as that of the gas conveying pipe, a plurality of groups of first temperature control blocks are arranged on the inner side of the outer ring plate, a plurality of groups of second temperature control blocks are arranged on the outer side of the inner ring plate corresponding to the first temperature control blocks, the first temperature control blocks and the second temperature control blocks are connected through two different third semiconductors, and one of the two third semiconductors is electrically connected with a power supply. The third semiconductor is a P-type semiconductor and an N-type semiconductor, the temperature control ring is embedded in the gas pipe, the gas pipe is divided into two sections, the temperature control ring provides support for installation of the inner ring plate and the outer ring plate, an air exchange space exists between the temperature control ring and the outer ring plate, the air exchange space provides a space for air to flow between the temperature control ring and the outer ring plate, the air exchange space is communicated with an external pipeline, when cold air is in the air exchange space, the air exchange space can be communicated with a waste water flow space below the impurity removing plate, the temperature of the impurity removing plate is further reduced, but when hot air is in the air exchange space, the hot air can be used for temperature regulation of a biological cell growth environment and used for improving the temperature of biological cell growth, for example, when biological cells are cultured in winter. The outer ring plate and the inner ring plate provide support for the installation of the first temperature control block and the second temperature control block, the first temperature control block and the second temperature control block are equivalent to a hot end and a cold end or a cold end and a hot end of a Peltier effect, and the second temperature control block cools or heats the inner ring plate through heat absorption or heat dissipation to enable the inner ring plate to cool or heat air.

Two groups of screw nuts are arranged between the drainage plate and the other group of movable plates and above the sealing layer, a supporting plate is arranged between the two groups of screw nuts, the air outlet pipe is arranged in the middle of the supporting plate, the lower end of the air outlet pipe is connected with the middle of the sealing layer, a moving chute is formed in the middle of the upper end of the water replenishing tank, and the upper end of the air outlet pipe is located in the moving chute. The lead screw rotates and makes the nut drive the outlet duct round trip movement on the surface of water under the motor is electronic, when the outlet duct removed towards the direction of drainage plate, during hot-air entered into the intake pipe through the outlet duct after replenishing a small amount of moisture, constantly toward the direction motion of keeping away from the drainage plate when the outlet duct, moisture in the hot-air was more and more, and humidity is more and more big, realizes the regulation to air humidity through the position of adjusting the outlet duct.

The inside top that is located the incubator of box is provided with the inlet plate, a plurality of groups gas exchange hole has been seted up on the inlet plate, and the inlet plate lower extreme is provided with two sets of slide rails, the position that corresponds the slide rail on the incubator is provided with the slider, the slider is located the slide rail, one side that lies in the incubator in the box is provided with the baffle, the intake pipe is located between baffle and the box, and the other end of intake pipe runs through the baffle and is located the inlet plate top. The incubator slides in the box through slider and slide rail to make things convenient for the staff to take the incubator out from the box, the baffle blocks the position of incubator, prevents that the incubator from sliding in the box, and the inlet plate is located the below of the intake pipe port of giving vent to anger, and the inlet plate makes the air after the humidification enter into the incubator through a plurality of groups gas exchange hole.

Compared with the prior art, the invention has the following beneficial effects:

1. mechanism takes a breath successively heats and cools down the processing through the peltier effect to the air, make moisture in the air condense and with air separation, mechanism takes a breath adsorbs the dust in the air through the water droplet that cools down the condensation, thereby reach air-purifying's effect, and dry the air, thereby the convenience carries out accurate control to the humidity of air, the moisturizing case supplements moisture to dry air, the moisturizing case controls the moisture that contains in the air, thereby reach accurate control air humidity's purpose, and then reach the accurate control of biological cell growing environment.

2. The sterilization polarization case heats the air, makes the air inflation to make the distance between bacterium and the dust enlarge, make the bacterium expose in the air, the bacterium is when through the electric field, and the electric current that makes flow between the electric field kills, and the edulcoration case is cooled down to the hot-air, makes the moisture in the air meet the condensation knot, makes dust, bacterium in the air and the water droplet fusion that condenses, thereby realizes the purification and the dehydration drying to the air.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic front view of the overall structure of the present invention;

FIG. 2 is a schematic internal front view of the overall structure of the present invention;

FIG. 3 is a schematic internal left view of the overall structure of the present invention;

FIG. 4 is a schematic view showing a connection structure of a polymerization tube and a trash box according to the present invention;

FIG. 5 is a schematic view of the internal structure of the trash box of the present invention;

FIG. 6 is a schematic view of the internal structure of the sterilization polarization box of the present invention;

FIG. 7 is a schematic top view of a sterilization polarization plate of the present invention;

FIG. 8 is a schematic front half-section view of a sterilization polarizing plate of the present invention;

FIG. 9 is a schematic top view of the vent pipe of the present invention;

FIG. 10 is a simulated view of the field shaft of the present invention in a snorkel to heat block connection;

FIG. 11 is a schematic view of the internal structure of the temperature control ring of the present invention;

fig. 12 is a schematic view of the internal structure of the refill tank of the present invention.

In the figure: 1. a box body; 2. a ventilation mechanism; 3. a water replenishing tank; 1-1, an incubator; 1-2, an air inlet plate; 1-3, bearing plates; 1-4, a baffle; 1-5, an air inlet pipe; 2-1, an air inlet cover; 2-2, sterilizing and polarizing box; 2-3, a conveying pipe; 2-4, removing impurities; 2-5, a drain pipe; 2-6, a gas transmission pipe; 2-7, a fan; 2-8, temperature control ring; 2-21, fixing ring; 2-22, sterilizing and polarizing plate; 2-23, a vent pipe; 2-24, a heating block; 2-25, electric field axis; 2-26, a heated block; 2-31, a polymeric tube; 2-32, folding the ring; 2-33, expansion groove; 2-41, a support plate; 2-42, an impurity removing plate; 2-43, a refrigeration plate; 2-44, a water outlet; 2-45, exhaust port; 2-81, outer ring plate; 2-82, inner ring plate; 2-83, a first temperature control block; 2-84, a second temperature control block; 3-1, a movable plate; 3-2, fixing plates; 3-3, moving the sliding chute; 3-4, a drainage plate; 3-5, sealing layer; 3-6, screw rod nut; 3-7 and an air outlet pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 12, the present invention provides a technical solution: the utility model provides a biological cell breeding device for cultivateing, survey, this biological cell breeding device includes box 1, installs in box 1 and takes a breath mechanism 2, moisturizing case 3, takes a breath mechanism 2 and moisturizing case 3 pipe connection, moisturizing case 3 and box 1 pipe connection, and mechanism 2 takes a breath carries out the sterilization and removes dust to the air and handles, and moisturizing case 3 supplyes moisture to the air.

The middle part in the box body 1 is provided with a bearing plate 1-3, the bearing plate 1-3 is provided with an incubator 1-1 in a sliding way, the left end of the bearing plate 1-3 is provided with an air inlet pipe 1-5, and one end of the air inlet pipe 1-5 is positioned above the incubator 1-1.

An air inlet plate 1-2 is arranged above an incubator 1-1 inside a box body 1, a plurality of groups of air exchange holes are formed in the air inlet plate 1-2, two groups of sliding rails are arranged at the lower end of the air inlet plate 1-2, a sliding block is arranged on the incubator 1-1 and corresponds to the sliding rails and is positioned in the sliding rails, a baffle plate 1-4 is arranged on the left side of the incubator 1-1 in the box body 1, an air inlet pipe 1-5 is positioned between the baffle plate 1-4 and the box body 1, and the other end of the air inlet pipe 1-5 penetrates through the baffle plate 1-4 and is positioned above the air inlet plate 1-2.

A box door is arranged at a position, corresponding to the incubator 1-1, on the box body 1, a transverse plate (not shown in the figure) for placing a culture dish is arranged in the middle of the incubator 1-1, an air outlet is formed below the transverse plate on the end surface of one side, close to the baffle plate 1-4, of the incubator 1-1, a connecting pipe (not shown in the figure) is arranged at the outer side of the air outlet, an exhaust pipe (not shown in the figure) is arranged at a position, corresponding to the air outlet, on the baffle plate 1-4, an end cover of the exhaust pipe is sleeved on the connecting pipe and is in sliding connection with the connecting pipe, the other end of the exhaust pipe is communicated with the space at the.

The box body 1 is also provided with a control system, a temperature sensor, a humidity sensor, a PH value detection device and other devices for detecting the cultivation of biological cells, the humidity sensor detects the humidity of air flowing out of the air inlet pipe 1-2 and the air inlet pipe 1-5, the temperature sensor detects the temperature of the air flowing out of the air inlet pipe 1-5, the control system controls the current connected with the temperature control ring 2-8 and controls the rotation of the screw nuts 3-6, when the cultivated biological cells need to be measured, the box door is opened, and the incubator 1-1 is taken out of the box body 1 to measure related work.

The air exchange mechanism 2 comprises two groups of fans 2-7, two groups of air inlet covers 2-1, two groups of sterilization polarization boxes 2-2 and an impurity removal box 2-4, wherein each group of fans 2-7 is fixed with the box body 1, a filter screen (not shown in the figure) is arranged on the box body 1 and positioned on the outer side of each fan 2-7, each group of air inlet covers 2-1 is arranged on each fan 2-7, one end of each group of sterilization polarization boxes 2-2 is respectively connected with the other end of each group of air inlet covers 2-1 through a pipeline, and the other end of each group of sterilization polarization boxes 2-2 is respectively connected with the impurity removal box 2-4 through a pipeline.

A fixed ring 2-21 is fixed in a sterilization polarization box 2-2, a sterilization polarization plate 2-22 is arranged above the fixed ring 2-21, a group of vertical installation grooves are arranged in the middle of the sterilization polarization plate 2-22, a plurality of other arc-shaped installation grooves are arranged on the sterilization polarization plate 2-22 and positioned on the outer side of the middle vertical installation groove, the inner wall of each installation groove is coated with heat insulating materials, a plurality of groups of installation grooves are provided with vent pipes 2-23, a group of vent pipes 2-23 in the middle are straight pipelines, the vent pipes 2-23 at other positions are all of arc structures, a plurality of groups of heating blocks 2-24 are arranged on the outer side of each vent pipe 2-23, each group of heating blocks 2-24 is connected with two different first semiconductors, and the other ends of the plurality of groups of first semiconductors are connected with an impurity removal, one of the first semiconductors is electrically connected to a power source.

The wall of the air pipe 2-23 is provided with a plurality of groups of through holes, a plurality of groups of electric field shafts 2-25 are arranged in the air pipe 2-23 through the through holes, the electric field shafts 2-25 are not contacted with the air pipe 2-23, two ends of the electric field shafts 2-25 are arranged on the sterilization polarization plates 2-22, the plurality of groups of electric field shafts 2-25 are divided into three groups, each group is respectively provided with two small groups of electric field shafts 2-25 in the horizontal direction and the vertical direction in the vertical direction, the horizontal and longitudinal electric field shafts 2-25 form a structure similar to a filter screen, and the electric field shafts 2-25 block the air flow in the air pipe 2-23, so that the air flow speed is reduced.

A plurality of groups of heating blocks 2-26 are arranged in the ventilating pipe 2-23, two different second semiconductors are arranged on each group of heating blocks 2-26, the plurality of groups of heating blocks 2-26 are connected in parallel through the second semiconductors, and the plurality of groups of second semiconductors are connected with the plurality of groups of electric field shafts 2-25.

The upper end of each group of sterilization polarization boxes 2-2 is provided with a conveying pipe 2-3, each group of conveying pipes 2-3 comprises a polymerization pipe 2-31, the other ends of the two groups of polymerization pipes 2-31 are communicated with the inside of the impurity removal box 2-4, a plurality of groups of furling rings 2-32 are arranged inside the polymerization pipes 2-31, and an expansion groove 2-33 is arranged between each two groups of furling rings 2-32 inside the polymerization pipes 2-31.

The inside of the impurity removing box 2-4 is provided with an arc-shaped supporting plate 2-41, the supporting plate 2-41 is provided with a plurality of groups of air vents, the V-shaped inverted impurity removing plate 2-42 is arranged above the supporting plate 2-41, the lower end of the impurity removing plate 2-42 is provided with a plurality of groups of water through holes, the inner side of the impurity removing plate 2-42 is provided with two groups of refrigerating plates 2-43, and the two groups of refrigerating plates 2-43 are electrically connected with the other ends of the first semiconductors.

A water outlet 2-44 is arranged on the impurity removing box 2-4 corresponding to the impurity removing plate 2-42, an air outlet 2-45 is arranged on the impurity removing box 2-4 and below the water outlet 2-44, a water outlet pipe 2-5 is arranged on the outer side of the impurity removing box 2-4 corresponding to the water outlet 2-44, an air pipe 2-6 is arranged on the outer side of the impurity removing box 2-4 corresponding to the air outlet 2-45, the air pipe 2-6 is connected with a water supplementing box 3 through a pipeline, and a partition plate is arranged inside the impurity removing box 2-4 and between a polymerization pipe 2-31 and a support plate 2-41.

The air delivery pipe 2-6 is provided with a temperature control ring 2-8, the air delivery pipe 2-6 is divided into two sections by the temperature control ring 2-8, an inner ring plate 2-82 and an outer ring plate 2-81 are arranged in the temperature control ring 2-8 from inside to outside, an air exchange space is formed by the temperature control ring 2-8 and the outer ring plate 2-81 in a matching way, the outer side of the temperature control ring 2-8 is communicated with an external pipeline (not shown in the figure) which is used for communicating the air exchange space, the pipe diameter of the inner ring plate 2-82 is the same as that of the air delivery pipe 2-6, a plurality of groups of first temperature control blocks 2-83 are arranged on the inner side of the outer ring plate 2-81, a plurality of groups of second temperature control blocks 2-84 are arranged on the outer side of the inner ring plate 2-82 corresponding to the positions of the first temperature control blocks 2-83, the plurality of groups of first temperature control blocks 2-83 are connected with, one of the two different third semiconductors is electrically connected to a power source.

The left and the right of the inside of the water replenishing tank 3 are respectively provided with a fixed plate 3-2, one side of each group of fixed plates 3-2 close to the water replenishing tank 3 is provided with a movable plate 3-1 in a sliding way, the fixed plate 3-2 is connected with the movable plate 3-1 in a sealing way, the upper end of the right movable plate 3-1 is provided with a drainage plate 3-4, the upper end of the right movable plate 3-1 is positioned below the drainage plate 3-4 and is provided with an air inlet groove, the drainage plate 3-4, the movable plate 3-1 below the drainage plate 3-4, the fixed plate 3-2 on the right side and the water replenishing tank 3 are matched with each other to form an air flowing space in the water replenishing tank 3, an air pipe 2-6 is communicated with the air flowing space, the right end of the drainage plate 3-4 is connected with the water replenishing tank 3 in a, the sealing layers 3-5 are rubber layers.

Two groups of screw rod nuts 3-6 are arranged between the drainage plate 3-4 and the left movable plate 3-1 and above the sealing layer 3-5, a motor (not shown) is arranged at the position corresponding to the screw rod outside the movable plate 3-1 and connected with the screw rod shaft, a supporting plate is arranged between the nuts of the two groups of screw rod nuts 3-6, an air outlet pipe 3-7 is arranged in the middle of the supporting plate, the lower end of the air outlet pipe 3-7 is connected with the middle of the sealing layer 3-5, a movable sliding groove 3-3 is arranged in the middle of the upper end of the water replenishing tank 3, the upper end of the air outlet pipe 3-7 is arranged in the movable sliding groove 3-3, and the air outlet.

The working principle of the invention is as follows:

the fan 2-7 extracts outside air and infuses the sterilization polarization box 2-2 through the air inlet cover 2-1, the air enters the vent pipe 2-23, the heating block 2-24 heats the vent pipe 2-23 under the Peltier effect, the heated block 2-26 absorbs heat in the vent pipe 2-23 and generates current through the second semiconductor under the Seebeck effect, and an electric field is generated among a plurality of groups of electric field axes 2-25 due to the distribution of positive electrons and negative electrons.

The electric field axis 2-25 blocks the air flow, at the same time, the inside of the air pipe 2-23 is heated to expand the air, the air density in the air pipe 2-23 is reduced, the distance between the dust and the bacteria is enlarged, when the dust and the bacteria flow through the electric field axis 2-25, the dust is polarized by a plurality of groups of electric fields, and the bacteria are killed by the electric current between the electric fields.

Air passing through the sterilization polarization box 2-2 enters the conveying pipe 2-3 and enters the polymerization pipe 2-31, and dust in the air is continuously gathered together to form dust particles under the action of the gathering ring 2-32 and the expansion groove 2-33 and is adhered to bacteria.

Hot air enters the impurity removing box 2-4 through the polymerization pipe 2-31 and impacts the impurity removing plate 2-42, the refrigeration plate 2-43 absorbs heat under the Peltier effect to reduce the temperature of the impurity removing box 2-4, so that the temperature in the impurity removing box 2-4 is reduced, the hot air impacts the low-temperature impurity removing plate 2-42, moisture in the hot air is condensed into water on the impurity removing plate 2-42, meanwhile, dust particles, bacteria and the like are attached to water drops, the water drops slide on the impurity removing plate 2-42 and flow to grooves of the supporting plate 2-41 along with the continuous entering of the hot air, and the water drops scour the dust particles and the bacteria in the sliding process, so that the dust particles and the bacteria enter the water discharge pipe 2-5 along with the water flow and are discharged out of the device.

The cold air without dust and bacteria enters the air delivery pipe 2-6 through the supporting plate 2-41, the inner ring plate 2-82 in the temperature control ring 2-8 is heated under the Peltier effect, the temperature control ring 2-8 heats the air, the heated air enters the air flowing space and flows below the sealing layer 3-5 under the guiding of the drainage plate 3-4, the hot air flows on the surface of the water under the pressing of the sealing layer 3-5 and evaporates the surface of the water, so that the hot air is supplemented with moisture, when the hot air supplemented with moisture flows below the air outlet pipe 3-7, the hot air flows into the air inlet pipe 1-5 through the air outlet pipe 3-7 and flows to the upper part of the air inlet plate 1-2 under the transmission of the air inlet pipe 1-5, the hot air enters the culture box 1-1 through the ventilation holes, heat, air and moisture are supplied for the culture of the biological cells, and the air in the incubator 1-1 flows out of the apparatus through the exhaust pipe.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological cell culture device for culture and assay, characterized in that: the biological cell cultivating device comprises a box body (1), wherein an air exchange mechanism (2) and a water replenishing tank (3) are arranged in the box body (1), the air exchange mechanism (2) is in pipeline connection with the water replenishing tank (3), the water replenishing tank (3) is in pipeline connection with the box body (1), the air exchange mechanism (2) is used for sterilizing and dedusting air, and the water replenishing tank (3) is used for replenishing moisture to the air;

a bearing plate (1-3) is arranged in the box body (1), an incubator (1-1) is arranged on the bearing plate (1-3), an air inlet pipe (1-5) is arranged at one end of the bearing plate (1-3), and one end of the air inlet pipe (1-5) is positioned above the incubator (1-1);

the air exchange mechanism (2) comprises two groups of fans (2-7), two groups of air inlet covers (2-1), two groups of sterilization polarization boxes (2-2) and impurity removal boxes (2-4), wherein each group of fans (2-7) is fixed with the box body (1), each group of air inlet covers (2-1) is arranged on the fans (2-7), one end of each sterilization polarization box (2-2) is connected with the other end of each air inlet cover (2-1) through a pipeline, the other ends of the two groups of sterilization polarization boxes (2-2) are connected with the impurity removal boxes (2-4) through pipelines, and the impurity removal boxes (2-4) are connected with the water supplement box (3) through pipelines;

fixing plates (3-2) are arranged on two sides inside the water replenishing tank (3), a movable plate (3-1) is arranged on each fixing plate (3-2), a drainage plate (3-4) is arranged at the upper end of one movable plate (3-1), the movable plates (3-1) below the drainage plate (3-4) and the fixed plates (3-2) and the water replenishing tank (3) above the drainage plate (3-4) are matched with each other to form an air flowing space inside the water replenishing tank (3), the impurity removing tank (2-4) is communicated with the air flowing space, one end of the drainage plate (3-4) is connected with the water replenishing tank (3) in a sliding manner, a sealing layer (3-5) is arranged between the other end of the drainage plate (3-4) and the other movable plate (3-1), an air outlet pipe (3-7) is arranged on the sealing layer (3-5), and the air outlet pipe (3-7) is connected with an air inlet pipe (1-5) through a pipeline.

2. A biological cell culturing device for culturing and assaying according to claim 1, characterized in that: the sterilization polarization box is characterized in that a fixing ring (2-21) is arranged in the sterilization polarization box (2-2), a sterilization polarization plate (2-22) is arranged above the fixing ring (2-21), a group of vertical vent pipes (2-23) are arranged in the middle of the sterilization polarization plate (2-22), a plurality of groups of other arc vent pipes (2-23) are arranged on the sterilization polarization plate (2-22) and located on the outer side of the middle vent pipes (2-23), a plurality of groups of heating blocks (2-24) are arranged on the outer side of each vent pipe (2-23), two different first semiconductors are arranged on each heating block (2-24), and the other ends of the first semiconductors are connected with an impurity removal box (2-4).

3. A biological cell culturing device for culturing and assaying according to claim 2, characterized in that: the sterilization device is characterized in that a plurality of groups of electric field shafts (2-25) are arranged inside the ventilating pipes (2-23), two ends of the plurality of groups of electric field shafts (2-25) are fixed with the sterilization polarization plates (2-22), a plurality of groups of heating blocks (2-26) are arranged inside the ventilating pipes (2-23), two different second semiconductors are arranged on the heating blocks (2-26), the plurality of groups of heating blocks (2-26) are connected in parallel through the second semiconductors, and the plurality of groups of second semiconductors are connected with the plurality of groups of electric field shafts (2-25).

4. A biological cell culturing device for culturing and assaying according to claim 3, characterized in that: the upper end of each group of the sterilization polarization boxes (2-2) is provided with a conveying pipe (2-3), each group of the conveying pipes (2-3) comprises a polymerization pipe (2-31), the other end of the polymerization pipe (2-31) is communicated with an impurity removal box (2-4), a plurality of groups of furling rings (2-32) are arranged inside the polymerization pipe (2-31), and an expansion groove (2-33) is formed in the polymerization pipe (2-31) and positioned between each two groups of furling rings (2-32).

5. A biological cell culturing device for culture and assay according to claim 4, wherein: an arc-shaped supporting plate (2-41) is arranged inside the impurity removing box (2-4), an inverted V-shaped impurity removing plate (2-42) is arranged above the supporting plate (2-41), two groups of refrigerating plates (2-43) are arranged on the impurity removing plate (2-42), the two groups of refrigerating plates (2-43) are electrically connected with the other ends of a plurality of groups of first semiconductors, one group of first semiconductors is electrically connected with a power supply, a water outlet (2-44) is arranged on the impurity removing box (2-4) corresponding to the impurity removing plate (2-42), an air exhaust port (2-45) is arranged on the impurity removing box (2-4) below the water outlet (2-44), a water drainage pipe (2-5) is arranged on the outer side of the impurity removing box (2-4) corresponding to the water outlet (2-44), the position, corresponding to the air outlet (2-45), of the outer side of the impurity removing box (2-4) is provided with an air conveying pipe (2-6), the air conveying pipe (2-6) is in pipe connection with the water replenishing box (3), a plurality of groups of air holes are formed in the supporting plate (2-41), a plurality of groups of water holes are formed in the lower end of the impurity removing plate (2-42), and a partition plate is arranged between the polymerization pipe (2-31) and the supporting plate (2-41) inside the impurity removing box (2-4).

6. A biological cell culturing device for culture and assay according to claim 5, wherein: the temperature control device is characterized in that the gas transmission pipe (2-6) is provided with a temperature control ring (2-8), an inner ring plate (2-82) and an outer ring plate (2-81) are arranged inside the temperature control ring (2-8) from inside to outside, the temperature control ring (2-8) and the outer ring plate (2-81) are matched with each other to form a ventilation space, the pipe diameter of the inner ring plate (2-82) is the same as that of the gas transmission pipe (2-6), a plurality of groups of first temperature control blocks (2-83) are arranged on the inner side of the outer ring plate (2-81), a plurality of groups of second temperature control blocks (2-84) are arranged on the outer side of the inner ring plate (2-82) corresponding to the positions of the first temperature control blocks (2-83), and the plurality of groups of first temperature control blocks (2-83) and the plurality of groups of second temperature control blocks (2-84) are connected, one of the two third semiconductors is electrically connected with a power supply.

7. A biological cell culturing device for culture and assay according to claim 6, wherein: two groups of screw rod nuts (3-6) are arranged between the drainage plate (3-4) and the other group of movable plates (3-1) and above the sealing layer (3-5), a supporting plate is arranged between the two groups of screw rod nuts (3-6), the air outlet pipe (3-7) is arranged in the middle of the supporting plate, the lower end of the air outlet pipe (3-7) is connected with the middle of the sealing layer (3-5), the middle of the upper end of the water replenishing tank (3) is provided with a movable chute (3-3), and the upper end of the air outlet pipe (3-7) is positioned in the movable chute (3-3).

8. A biological cell culturing device for culturing and assaying according to claim 7, wherein: an air inlet plate (1-2) is arranged above an incubator (1-1) inside the incubator body (1), a plurality of groups of air exchange holes are formed in the air inlet plate (1-2), two groups of sliding rails are arranged at the lower end of the air inlet plate (1-2), a sliding block is arranged on the incubator (1-1) corresponding to the sliding rails and located in the sliding rails, a baffle (1-4) is arranged on one side, located on the incubator (1-1), in the incubator body (1), the air inlet pipe (1-5) is located between the baffle (1-4) and the incubator body (1), and the other end of the air inlet pipe (1-5) penetrates through the baffle (1-4) and is located above the air inlet plate (1-2).