Summary of the invention
In order to overcome above-mentioned defective, the purpose of this invention is to provide a kind of cell culture apparatus, this device can place identical common lab or production plant to carry out isolated operation with other normal experiment instrument and equipments together, and the clean rank of environment is not had special requirement.
To achieve these goals, the present invention adopts following technical scheme:
Cell culture apparatus, it comprises Controlling System, ultraviolet lamp, equipment box, cell culture apparatus, and Controlling System is installed in the equipment box, and culturing room is set on the equipment box, and cell culture apparatus is installed in the culturing room.
In culturing room, the filling system that is applied to cell cultures can be installed as required.
Top in culturing room is provided with the air filter of being made up of blower fan and filtration unit.Wherein, air filter is motor installed device casing top, and filtration unit is installed on the top of culturing room.
Cell culture apparatus is a torrent formula bio-reactor, it comprises support, bearing, culture tank, culture bag, electric motor, excentric shaft, it is characterized in that: excentric shaft by bearing fixing between support and vibrator shaking tray, the bottom of culture tank is fixed on the vibrator shaking tray, electric motor connects drive shaft by belt, and culture bag places cultivates in the tank body.
Above-mentioned vibrator shaking tray is done vertical direction stepless speed regulation by the amplitude of design and is rotated.
Above-mentioned culture tank, culture bag are the rounding stage body.
Be applied to the filling system of cell cultures, it comprises support, support lift adjustment button, peristaltic pump, guide pillar, manual adjustment wheel, filling type culture bag container, filling type culture bag container is fixed between two guide pillars on the support by anchor, the guide pillar lower end is provided with support lift adjustment button, peristaltic pump is fixed in the cupboard, and manual adjustment wheel is regulated the portable plate that is fixed on the guide pillar.
Controlling System comprises pilot circuit and automatic control program, and pilot circuit comprises PLC, touch-screen, automatically controlled plate, peristaltic pump, blower fan, magnetic valve, liquid level pump, pneumatic pump, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly., heating film, perfusion lifting support, vibrator.Automatically controlled plate is connected with peristaltic pump, blower fan, magnetic valve, liquid level pump, pneumatic pump respectively, PLC is connected with touch-screen, automatically controlled plate, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly. respectively, the Frequency Converter Control vibrator, SF driver control perfusion lifting support, rly. control heating film.
One, system is divided into manual control and controls two kinds of operator schemes automatically.
Manual master mode: manually under the master mode, can be by the corresponding operating of upper computer operation control system, each parameter in the regulation system incubator makes the environment of incubator be fit to the needs of cell cultures.
Automatic control mode: according to the cell cultures needs, preestablish required each parameter of cell cultures, system regulates the environment in the incubator by self-regulating mode, and cell is freely cultivated and the required environment of growing to satisfy.
Two, the automatic control program of torrent formula bio-reactor
Before gathering first, temperature, pH, dissolved oxygen signal all need to carry out calibration operation.Simultaneously, also all need to calibrate for good harvest pump and fluid infusion pump, so that the amount of liquid in the incubator roughly keeps balance.
1, to temperature controlling:
Adopt PT100 to transfer the analog input channel that the 4-20mA current signal enters system to through transmitter.System is converted into corresponding temperature value automatically.And temperature value and default value that handle is gathered compare.If the collection value is much smaller than set(ting)value: the temperature heater valve is often opened.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, periodically the break-make of control output heater valve.Sampled value is greater than set(ting)value: system's heater valve disconnects.
2, to the control of PH:
The PH transmitter transfers the signal that collects to analog input channel that the 4-20mA current signal enters system through transmitter.System is converted into corresponding pH value automatically.
1) for the operation of the PH upper limit:
If the collection value is much larger than the set(ting)value upper limit: fill CO
2Valve often leaves.If sampled value greater than the set(ting)value upper limit within the specific limits: system carries out the PWM mode and controls, periodically control output CO
2The break-make of valve.Sampled value is less than the set(ting)value upper limit: fill CO
2Valve disconnects.
2) for the operation of PH lower limit:
If the collection value is much smaller than set(ting)value: add the alkali pump valve and often open.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, and periodically control output adds the break-make of alkali pump valve.Sampled value is greater than set(ting)value: system adds the alkali pump valve and disconnects.
3) for sampled value between the PH bound:
CO
2Valve and alkali addition valve are not opened.
3, to the control of dissolved oxygen:
The oxygen content transmitter transfers the signal that collects to analog input channel that the 4-20mA current signal enters system through transmitter.System is converted into corresponding oxygen dissolving value automatically.
1) for the operation of the dissolved oxygen upper limit:
If the collection value is much larger than the set(ting)value upper limit: fill N
2(nitrogen) valve is often opened.If sampled value greater than the set(ting)value upper limit within the specific limits: system carries out the PWM mode and controls, periodically control output N
2The break-make of valve.Sampled value is less than the set(ting)value upper limit: fill N
2Valve disconnects.
2) for the operation of dissolved oxygen lower limit:
If the collection value is much smaller than set(ting)value: add O
2(oxygen) valve is often opened.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, periodically control output O
2The break-make of valve.Sampled value is greater than set(ting)value: system O
2The valve valve disconnects.
3) for sampled value between the dissolved oxygen bound:
N
2Valve and O
2Valve is not opened.
4, for the good harvest of system, the control of fluid infusion:
At the nutrient solution that consumes in the incubator always, system needs regularly cover a certain amount of nutrient solution to guarantee the growth of cell to incubator in the process of cell cultures.For making in the incubator contained amount of liquid constant substantially, good harvest amount and amount infused need reach a balance substantially, and this target can realize by system's pump nutrient solution of having a good harvest in fluid infusion of having a good harvest.
System calculates the time that each divides master slave system need carry out fluid infusion or good harvest automatically by a corresponding fluid infusion total amount or good harvest total amount and time corresponding total amount are set.The work of fluid infusion pump that therefore, to adopt above-mentioned this PWM mode be the may command correspondence or good harvest pump.
5, the setting of shaking speed and control:
Set given shaking speed signal by upper computer, system by the analog output channel, makes motor turn round by given rotating speed through the conversion back.In operation process, import the detection system actual speed by photoelectric detection switch.If actual speed and given rotating speed have deviation, system will adjust automatically, and actual speed value and setting tachometer value are complementary.
6, the warning of system output:
After the warning bounds such as temperature, pH, dissolved oxygen and shaking speed of system were set, if the actual parameter value of system is prescribed a time limit greater than the upper limit of reporting to the police or less than the following of warning, the system alarm signal promptly was triggered, and the hummer alarm is sounded.Show it is that what reason has triggered guard signal at upper computer simultaneously, so that operator search.
7, the automatic control program of perfusion servosystem
A) control of filling system liquid level:
The filling system liquid level adopts high low level photoelectric detection switch to control, and when system's liquid level was between a high position and low level, the filling system motor was not worked.Perfusion motor liquid feeding when detecting the low level signal makes liquid enter between the high low level and continuation operation certain hour; When detecting high signal, perfusion motor food taken late at night makes liquid enter between the high low level and continuation operation certain hour; Thereby the system liquid level of making is in a metastable liquid level zone all the time.It is interlocking that perfusion liquid feeding and perfusion depletion start.
B) filling system servomotor control:
Need to carry out earlier zero point correction in the filling system, set rising or falling speed and target location after, press executive button, filling system promptly moves towards the target location.
C) ultraviolet lamp, high low speed blower fan interlock control:
Manually under the running condition, preestablish the working time of ultraviolet lamp and high-speed fan.After pressing the start button startup, ultraviolet lamp moves to setting-up time earlier; Then, after blower fan moves for some time in the high speed mode, by the high speed steering low cruise to the scheduled time.In addition, operations such as ultraviolet lamp, fluorescent lamp, blower fan high speed, blower fan low speed all can be operated separately respectively in manual case, but blower fan high speed and two kinds of operation scheme interlockings of blower fan low speed.
Apparatus of the present invention can miniaturization, does not need independent aseptic house, can be applicable to the laboratory, also can be used for industrial production.
Embodiment
Further specify below in conjunction with accompanying drawing:
As shown in Figure 1, cell culture apparatus, it comprises Controlling System 1, cupboard 2, cell culture apparatus 3, and Controlling System 1 is installed in the cupboard 2, and culturing room 4 is set on the cupboard 2, and cell culture apparatus 3 is installed in the culturing room 4.The filling system 5 that is applied to cell cultures also can be installed in culturing room 4; At the top of culturing room 4 air filter 6 is set.
As shown in Figure 2, cell culture apparatus is a torrent formula vibrator, it comprises support 7, bearing 8, culture tank 9, culture bag 10, electric motor 11, excentric shaft 12, it is characterized in that: excentric shaft 12 is fixed between support 7 and the vibrator shaking tray 13 by bearing 8, the bottom of culture tank 9 is fixed on the vibrator shaking tray 13, electric motor 11 is by belt connection bearing 8, and culture bag 10 is fixed in the culture tank 9.
As shown in Figure 3, be applied to the filling system of cell cultures, it comprises support 14, peristaltic pump, guide pillar 17, motor 18, filling type culture bag container 19, filling type culture bag container 19 is fixed between two guide pillars 17 on the support 14 by anchor 15, peristaltic pump is fixedly in the cupboard 2, on motor 18 supports 14, motor 18 output shafts connect bull stick 16.
As shown in Figure 4, Controlling System comprises pilot circuit and automatic control program, and pilot circuit comprises PLC, touch-screen, automatically controlled plate, peristaltic pump, blower fan, magnetic valve, liquid level pump, pneumatic pump, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly., heating film, perfusion lifting support, vibrator.Automatically controlled plate is connected with peristaltic pump, blower fan, magnetic valve, liquid level pump, pneumatic pump respectively, PLC is connected with touch-screen, automatically controlled plate, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly. respectively, the Frequency Converter Control vibrator, SF driver control perfusion lifting support, rly. control heating film.