CN101603006B - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- CN101603006B CN101603006B CN200910100606A CN200910100606A CN101603006B CN 101603006 B CN101603006 B CN 101603006B CN 200910100606 A CN200910100606 A CN 200910100606A CN 200910100606 A CN200910100606 A CN 200910100606A CN 101603006 B CN101603006 B CN 101603006B
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- cell culture
- culture apparatus
- vibrator
- culturing room
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Abstract
The invention relates to a cell culture device, which is characterized in that the device comprises a control system, an equipment box body, an ultraviolet lamp and a cell culture apparatus, wherein the control system is arranged in the equipment box body; the inside of the equipment box body is a purification culture chamber; and the cell culture apparatus is arranged in the culture chamber. The device can be miniaturized, does not need individual sterile houses, can be applied to laboratories, and can also be used for industrial production.
Description
Technical field:
The present invention relates to a kind of cell culture apparatus.
Background technology
In the animal cell culture process, very high to environment requirement, need prevent microbiological contamination.Yet existing cell culture apparatus mostly places clean environment more than 100,000 grades, and scientific research institution or enterprise need special in animal cell culture makes up an independent laboratory or production plant, have increased investment and running cost virtually.
Summary of the invention
In order to overcome above-mentioned defective; The purpose of this invention is to provide a kind of cell culture apparatus; This device can place identical common lab or production plant to carry out isolated operation with other normal experiment instrument and equipments together, and the clean rank of environment is not had special requirement.
To achieve these goals, the present invention adopts following technical scheme:
Cell culture apparatus, it comprises system, uv lamp, equipment box, cell culture apparatus, and system is installed in the equipment box, and culturing room is set on the equipment box, and cell culture apparatus is installed in the culturing room.
In culturing room, installation application is in the filling system of cell cultures as required.
Top in culturing room is provided with the air filter of being made up of blower fan and filtration unit.Wherein, air filter is motor installed device casing top, and filtration unit is installed on the top of culturing room.
Cell culture apparatus is a torrent formula bio-reactor; It comprises support, bearing, culture tank, culture bag, phonomoter, excentric shaft; It is characterized in that: excentric shaft passes through bearing fixing between support and vibrator shaking tray; The bottom of culture tank is fixed on the vibrator shaking tray, and phonomoter connects drive shaft through belt, and culture bag places cultivates in the tank body.
Above-mentioned vibrator shaking tray is done vertical direction stepless speed regulation by the amplitude of design and is rotated.
Above-mentioned culture tank, culture bag are the rounding stage body.
Be applied to the filling system of cell cultures; It comprises support, support up-down adjusting knob, peristaltic pump, guide pillar, manual adjustment wheel, filling type culture bag container; Filling type culture bag container is fixed between two guide pillars on the support through anchor; The guide pillar lower end is provided with support up-down adjusting knob, and peristaltic pump is fixed in the cupboard, and manual adjustment wheel is regulated and is fixed on the portable plate on the guide pillar.
System comprises pilot circuit and automatic control program, and pilot circuit comprises PLC, touch-screen, automatically controlled plate, peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly., heating film, perfusion lifting support, vibrator.Automatically controlled plate is connected with peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump respectively; PLC is connected with touch-screen, automatically controlled plate, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly. respectively; The Frequency Converter Control vibrator; SF driver control perfusion lifting support, rly. control heating film.
One, system is divided into manual control and controls two kinds of operator schemes automatically.
Manual master mode: manually under the master mode, can be through the corresponding operating of upper computer operation control system, each parameter in the regulation system incubator makes the environment of incubator be fit to the needs of cell cultures.
Automatic control mode: according to the cell cultures needs, preestablish required each parameter of cell cultures, system regulates the environment in the incubator through self-regulating mode, and cell is freely cultivated and the required environment of growing to satisfy.
Two, the automatic control program of torrent formula bio-reactor
Before gathering first, temperature, pH, dissolved oxygen signal all need carry out calibration operation.Simultaneously, also all need calibrate for good harvest pump and fluid infusion pump, so that the amount of liquid in the incubator roughly keeps balance.
1, to temperature controlling:
Adopt PT100 to transfer the analog input channel that the 4-20mA current signal gets into system to through transmitter.System is converted into corresponding temperature value automatically.And temperature value and default value that handle is gathered compare.If the collection value is much smaller than set(ting)value: the temperature heater valve is often opened.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, periodically the break-make of control output heater valve.Sampled value is greater than set(ting)value: system's heater valve breaks off.
2, to the control of PH:
The PH transmitter transfers the signal that collects to analog input channel that the 4-20mA current signal gets into system through transmitter.System is converted into corresponding pH value automatically.
1) for the operation of the PH upper limit:
If the collection value is much larger than the set(ting)value upper limit: fill CO
2Valve often leaves.If sampled value greater than the set(ting)value upper limit within the specific limits: system carries out the PWM mode and controls, periodically control output CO
2The break-make of valve.Sampled value is less than the set(ting)value upper limit: fill CO
2Valve breaks off.
2) for the operation of PH lower limit:
If the collection value is much smaller than set(ting)value: add the alkali pump valve and often open.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, and periodically control output adds the break-make of alkali pump valve.Sampled value is greater than set(ting)value: system adds the alkali pump valve and breaks off.
3) for sampled value between the PH bound:
CO
2Valve and alkali addition valve are not opened.
3, to the control of dissolved oxygen:
The oxygen content transmitter transfers the signal that collects to analog input channel that the 4-20mA current signal gets into system through transmitter.System is converted into corresponding oxygen dissolving value automatically.
1) for the operation of the dissolved oxygen upper limit:
If the collection value is much larger than the set(ting)value upper limit: fill N
2(nitrogen) valve is often opened.If sampled value greater than the set(ting)value upper limit within the specific limits: system carries out the PWM mode and controls, periodically control output N
2The break-make of valve.Sampled value is less than the set(ting)value upper limit: fill N
2Valve breaks off.
2) for the operation of dissolved oxygen lower limit:
If the collection value is much smaller than set(ting)value: add O
2(oxygen) valve is often opened.If sampled value less than set(ting)value within the specific limits: system carries out the PWM mode and controls, periodically control output O
2The break-make of valve.Sampled value is greater than set(ting)value: system O
2The valve valve breaks off.
3) for sampled value between the dissolved oxygen bound:
N
2Valve and O
2Valve is not opened.
4, for the good harvest of system, the control of fluid infusion:
At the nutrient solution that consumes in the incubator always, system needs regularly cover a certain amount of nutrient solution to guarantee the growth of cell to incubator in the process of cell cultures.For making in the incubator contained amount of liquid constant basically, good harvest amount and amount infused need reach a balance basically, and this target can realize through system's pump nutrient solution of in fluid infusion, having a good harvest of having a good harvest.
System calculates the time that each divides master slave system need carry out fluid infusion or good harvest automatically through a corresponding fluid infusion total amount or good harvest total amount and time corresponding total amount are set.Therefore, adopting above-mentioned this PWM mode is the work of corresponding fluid infusion pump of may command or good harvest pump.
5, the setting of shaking speed and control:
Set given shaking speed signal through upper computer, system through the analog output channel, makes motor turn round by given rotating speed through the conversion back.In operation process, import the detection system actual speed through photoelectric detection switch.If actual speed and given rotating speed have deviation, system will adjust automatically, and actual speed value and setting tachometer value are complementary.
6, the warning of system output:
After the warning bounds such as temperature, pH, dissolved oxygen and shaking speed of system were set, if the actual parameter value of system is prescribed a time limit greater than the upper limit of reporting to the police or less than the following of warning, the system alarm signal promptly was triggered, and the hummer alarm is sounded.Show it is that what reason has triggered guard signal at upper computer simultaneously, so that operator search.
7, the automatic control program of perfusion servosystem
A) control of filling system liquid level:
The filling system liquid level adopts high low level photoelectric detection switch to control, and when system's liquid level was between a high position and low level, the filling system motor was not worked.Perfusion motor liquid feeding when detecting low makes liquid get between the high low level and continuation operation certain hour; When detecting high signal, perfusion motor food taken late at night makes liquid get between the high low level and continuation operation certain hour; Thereby the system liquid level of making is in a metastable liquid level zone all the time.It is interlocking that the perfusion liquid feeding starts with the perfusion depletion.
B) filling system servomotor control:
Need carry out zero point correction earlier in the filling system, set rising or falling speed and target location after, press executive button, filling system promptly moves towards the target location.
C) uv lamp, high low speed blower fan interlock control:
Manually under the running condition, preestablish the working time of uv lamp and high-speed fan.After pressing the start button startup, uv lamp moves to setting-up time earlier; Then, after blower fan moves for some time with the high speed mode, by the high speed steering SR to the scheduled time.In addition, operations such as uv lamp, fluorescent lamp, blower fan high speed, blower fan low speed all can be operated separately respectively in manual case, but blower fan high speed and two kinds of operation scheme interlockings of blower fan low speed.
Apparatus of the present invention can miniaturized, does not need the individual sterile house, can be applicable to the laboratory, also can be used for industrial production.
Description of drawings
Fig. 1 structural representation of the present invention;
Fig. 2 is a torrent cell reactor structure iron;
But Fig. 3 is the filling system structure iron;
Fig. 4 is the system skeleton diagram.
Embodiment
Further specify below in conjunction with accompanying drawing:
As shown in Figure 1, cell culture apparatus, it comprises system 1, cupboard 2, cell culture apparatus 3, and system 1 is installed in the cupboard 2, and culturing room 4 is set on the cupboard 2, and cell culture apparatus 3 is installed in the culturing room 4.But culturing room 4 also installation application in the filling system 5 of cell cultures; At the top of culturing room 4 air filter 6 is set.
As shown in Figure 2; Cell culture apparatus is a torrent formula vibrator; It comprises support 7, bearing 8, culture tank 9, culture bag 10, phonomoter 11, excentric shaft 12, it is characterized in that: excentric shaft 12 is fixed between support 7 and the vibrator shaking tray through bearing 8, and the bottom of culture tank 9 is fixed on the vibrator shaking tray; Phonomoter 11 is through belt connection bearing 8, and culture bag 10 is fixed in the culture tank 9.
As shown in Figure 3; Be applied to the filling system of cell cultures; It comprises support 14, peristaltic pump, guide pillar 17, motor 18, filling type culture bag container 19, and filling type culture bag container 19 is fixed between two guide pillars 17 on the support 14 through anchor 15, and peristaltic pump is fixedly in the cupboard 2; On motor 18 supports 14, motor 18 output shafts connect bull stick 16.
As shown in Figure 4; System comprises pilot circuit and automatic control program, and pilot circuit comprises PLC, touch-screen, automatically controlled plate, peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly., heating film, perfusion lifting support, vibrator.Automatically controlled plate is connected with peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump respectively; PLC is connected with touch-screen, automatically controlled plate, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly. respectively; The Frequency Converter Control vibrator; SF driver control perfusion lifting support, rly. control heating film.
Claims (7)
1. cell culture apparatus, it is characterized in that: comprise system, equipment box, uv lamp, cell culture apparatus, system is installed in the equipment box, and equipment box inside is culturing room, and cell culture apparatus is installed in the culturing room; Culturing room also installation application in the filling system of cell cultures; Be applied to the filling system of cell cultures; It comprises support, support up-down adjusting knob, peristaltic pump, guide pillar, manual adjustment wheel or drive-motor, filling type culture bag container; Filling type culture bag container is fixed between two guide pillars on the support through sliding rail; Guide pillar lower end or upper end configuration driven motor or manual adjustment wheel are regulated, culturing room's internal configuration peristaltic pump.
2. according to the said cell culture apparatus of claim 1; It is characterized in that: cell culture apparatus is a torrent formula vibrator; It comprises support, bearing, culture tank, culture bag, phonomoter, excentric shaft, and between support and vibrator shaking tray, the bottom of culture tank is fixed on the vibrator shaking tray excentric shaft through bearing fixing; Phonomoter connects drive shaft through belt, and culture bag is fixed in the culture tank.
3. according to the said cell culture apparatus of claim 2, it is characterized in that: above-mentioned vibrator shaking tray is done vertical direction stepless speed regulation by the amplitude of design and is rotated.
4. according to the said cell culture apparatus of claim 2, it is characterized in that: above-mentioned culture tank, culture bag are the rounding stage body.
5. according to the said cell culture apparatus of claim 1; It is characterized in that: at the top of culturing room the high-efficiency air filtering device is set, air filter is by blower fan, and air channel and filtration unit are formed; Blower fan cooperates the top of installed device casing with the air channel, filtration unit is installed the top of culturing room.
6. according to the said cell culture apparatus of claim 1; It is characterized in that: system comprises pilot circuit and automatic control program; Pilot circuit comprises PLC, touch-screen, automatically controlled plate, peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly., heating film, perfusion lifting support, vibrator; Automatically controlled plate is connected with peristaltic pump, blower fan, SV, liquid level pump, pneumatic pump respectively; PLC is connected with touch-screen, automatically controlled plate, electronic ruler, tachogenerator, DO transmitter, PH transmitter, T electrode, frequency transformer, SF driving mechanism, rly. respectively; The Frequency Converter Control vibrator, SF driver control perfusion lifting support, rly. control heating film.
7. according to the said cell culture apparatus of claim 1, it is characterized in that: through logical aseptic wind, ultra violet lamp can form 100,000 grades to hundred grades aseptic clean room in the cupboard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910100606A CN101603006B (en) | 2009-07-10 | 2009-07-10 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910100606A CN101603006B (en) | 2009-07-10 | 2009-07-10 | Cell culture device |
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| CN101603006A CN101603006A (en) | 2009-12-16 |
| CN101603006B true CN101603006B (en) | 2012-10-10 |
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| CN200910100606A Active CN101603006B (en) | 2009-07-10 | 2009-07-10 | Cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107532132A (en) * | 2015-02-09 | 2018-01-02 | 优尼沃赛尔斯公司 | For producing the systems, devices and methods of cell and/or cell products |
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| CN103194390B (en) * | 2013-04-19 | 2014-08-06 | 南方医科大学南方医院 | Double-loop oscillation perfusion-type biological reaction system |
| CN108138116A (en) * | 2015-08-25 | 2018-06-08 | 通用电气公司 | In biological manufacturing equipment and it is related to its improvement |
| CN106554918B (en) * | 2016-05-12 | 2019-04-16 | 厦门医学院 | A kind of microculture integration apparatus |
| WO2017202192A1 (en) * | 2016-05-27 | 2017-11-30 | 中国科学院广州生物医药与健康研究院 | Multi-purpose tool tip for stem cell induction and culturing apparatus, and stem cell induction and culturing apparatus |
| CN108018204A (en) * | 2016-11-03 | 2018-05-11 | 浙江金仪盛世生物工程有限公司 | A kind of parallel bioreactor system |
| CN107389961B (en) * | 2017-08-16 | 2019-11-08 | 领航基因科技（杭州）有限公司 | A kind of heating device applied to biosystem |
| CN109652299A (en) * | 2018-11-15 | 2019-04-19 | 上海量能生物科技有限公司 | Bioreactor with resistance to movement function |
| CN110093267A (en) * | 2019-05-31 | 2019-08-06 | 江苏普瑞康生物医药科技有限公司 | A kind of cell recovery instrument |
| CN111019826A (en) * | 2019-12-23 | 2020-04-17 | 深圳赛动生物自动化有限公司 | Stem cell centrifugal bin control system based on programmable controller and control method thereof |
| CN112980685B (en) * | 2021-04-20 | 2021-08-06 | 广州赛太特生物医学科技有限公司 | A biological cell breeding device for cultivateing, survey |
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Cited By (1)
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| CN107532132A (en) * | 2015-02-09 | 2018-01-02 | 优尼沃赛尔斯公司 | For producing the systems, devices and methods of cell and/or cell products |
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| CN101603006A (en) | 2009-12-16 |
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Owner name: ZHEJIANG JINYI SHENGSHI BIOLOGICAL ENGINEERING CO. Free format text: FORMER OWNER: HANGZHOU AMPROTEIN BIOENGINEERING CO., LTD. Effective date: 20140902 |
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Free format text: CORRECT: ADDRESS; FROM: 310019 HANGZHOU, ZHEJIANG PROVINCE TO: 313099 HUZHOU, ZHEJIANG PROVINCE |
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Effective date of registration: 20140902 Address after: 313099, room 3, building 1366, 1201-2 Hongfeng Road, Zhejiang, Huzhou Patentee after: Zhejiang Jinyi Shengshi Biological Engineering Co., Ltd. Address before: 310019, No. nine, 63 Ring Road, Jianggan science and Technology Park, Hangzhou, Zhejiang, Jianggan District Patentee before: Hangzhou AmProtein Bioengineering Co., Ltd. |