CN106554918A - A kind of microorganism cultivates integration apparatus - Google Patents

Abstract

The invention discloses an integrated equipment for cultivating microorganisms, which comprises a chamber, a constant temperature air bath circulation generator, an air filter, an ozone generator, an ultraviolet lamp, an operating table device, an oscillation device, a control system and the like arranged in the chamber. The operating table device is movably arranged in the chamber and used for inoculation operations. In the first state, it is unfolded to form a platform suspended in the chamber, and in the second state, it is folded on the side wall of the chamber; the oscillating device is used to drive the inoculated The culture flask of microorganisms performs horizontal repeated motion. The control system is used to control the actions of the above-mentioned devices to realize automatic and intelligent regulation. The integrated equipment of the present invention can perform multiple operations, ensures the continuity of the microorganism cultivation process, avoids bacterial contamination, and improves the success rate of microorganism cultivation.

Description

An integrated equipment for microbial cultivation

technical field

The invention relates to equipment, in particular to an integrated equipment for cultivating microorganisms.

Background technique

Microbial cultivation is of great significance in the fields of biology, medicine, food, sewage treatment, etc. The process of microbial cultivation is complex and cumbersome, and often requires step-by-step processing, requiring more equipment and switching between different equipment. Due to the strict requirements on temperature, humidity, and cleanliness during the microbial cultivation process, it is very easy to be contaminated when switching between different equipments, resulting in the failure of the cultivation. In addition, a variety of equipment not only takes up a lot of space, but also requires operators to use each equipment skillfully and regularly maintain and repair these equipment, which consumes a lot of manpower, material and financial resources, and also brings great difficulties to users. Therefore, the integration and intelligence of equipment is an urgent need for the development of the industry.

Contents of the invention

The invention provides an integrated equipment for cultivating microorganisms, which overcomes the shortcomings of the prior art for cultivating microorganisms.

The technical solution adopted by the present invention to solve its technical problems is: a kind of microbial cultivation integrated equipment, comprising:

A chamber, the chamber is provided with a movable window; a constant temperature air bath circulation generator, arranged at the bottom of the chamber, is used to generate a constant temperature circulating airflow in the chamber to maintain a temperature suitable for microbial cultivation; an air filter, It is installed on the top of the chamber to filter the constant-temperature circulating airflow entering the chamber; the ozone generator is arranged on the bottom of the chamber to generate ozone, and the generated ozone enters the constant-temperature circulating airflow to clean the chamber. Carry out ozone sterilization; the ultraviolet lamp is arranged on the top of the chamber, and is used to carry out ultraviolet disinfection on the chamber; the console device is movable in the chamber and is used for inoculation operation, and the console device is in a first state Expanding to form a platform suspended in the chamber, folded on the side wall of the chamber in a second state; the oscillating device is arranged in the chamber, and the oscillating device is provided with a number of fixed seats for placing culture bottles, To drive the culture bottle inoculated with microorganisms to perform horizontal repeated movements; the control system is used to control the operation of the constant temperature air bath cycle generator, air filter, ozone generator, ultraviolet lamp and oscillation device;

The use of the equipment for microbial cultivation includes the following steps: deploying the operating table device in the chamber and placing the equipment required for microbial inoculation; closing the movable window, and sequentially starting the constant temperature air bath circulation generator through the control system to generate constant temperature circulating air flow, the The air filter filters the constant temperature circulating airflow, and the ozone generator performs ozone disinfection; turn off the ozone generator, put in the culture bottle with the culture medium after 2-3 hours, close the movable window, and start the ultraviolet lamp for disinfection ; After the sterilization is completed, the inoculation is carried out in the box room, and the console device is put away after the inoculation, the inoculated culture bottle is placed on the fixed seat of the oscillation device, and the oscillation device is controlled by the control system to oscillate.

Preferably, the wall body of the chamber is made of stainless steel plate, and the movable window is a push-pull glass window whose opening degree can be adjusted up and down.

Preferably, the operating table device includes an operating panel and two movable frames, one side of the operating panel is rotatably connected to the side wall of the chamber, and the two movable frames are respectively hinged to the two ends of the opposite side of the operating panel and the side of the chamber Between the walls; the movable frame includes two connecting rods that are rotatably connected. In the first state, the two connecting rods rotate to 180° so that the operating panel can be extended horizontally. In the second state, the two connecting rods rotate to overlap so that the operation panel can be folded and attached to the side wall of the chamber.

Preferably, the other side of the operation panel and the side wall of the corresponding box chamber are provided with matching buckles, and the operation panel is fixed to the side wall of the box chamber through the buckles in the second state.

Preferably, the operating platform device includes an operating panel and two poles, one side of the operating panel is rotatably connected to the side wall of the chamber, and the two poles are hinged to the side wall of the chamber below the operating panel in a horizontally rotatable manner; In the first state, the two poles rotate to be perpendicular to the side wall of the chamber and the operating panel is erected on the poles. In the second state, the two poles rotate to fit the side wall of the chamber. Sagging to fit the side wall of the chamber.

Preferably, the oscillating device includes a carrying platform and a driving mechanism for driving the carrying platform to perform horizontal reciprocating motion, the fixed seat is arranged on the carrying platform, and the driving mechanism is connected to and controlled by the control system.

Preferably, the carrying platform is provided with several through holes.

Preferably, the control system includes a main control module, a power supply module and an input module; the main control module receives instructions input from the input module and controls the constant temperature air bath cycle generator, air filter, ozone generator, ultraviolet lamp and oscillation device accordingly action; the power module is used for power supply.

Preferably, the main control module is a micro control unit, and the input module is a touch screen.

Preferably, fluorescent lamps are also arranged in the box chamber.

Compared with the prior art, the present invention has the following beneficial effects:

One, the box room of the integrated equipment of the present invention is provided with a constant temperature air bath circulation generator, an air filter, an ozone generator and an ultraviolet lamp, etc., which are used for disinfection and sterilization, so that the box room can maintain a dust-free, aseptic ultra- Clean and constant temperature environment; the box room is equipped with an oscillating device and a movable console device. The console device is unfolded to facilitate inoculation and other operations. It can be folded on the side wall during subsequent shaking culture, without occupying space. After inoculation The culture bottle can be directly placed on the oscillating device to effectively avoid bacterial contamination; the integrated equipment of the present invention can perform various operations, ensuring the continuity of the microorganism cultivation process and improving the success rate of microorganism cultivation.

Second, through the control system to control the work of the constant temperature air bath circulation generator and the oscillation device in the box, and connect the main control unit through the touch screen to operate, the disinfecting, oscillation, etc. can be set according to the growth habits of specific microorganisms. The temperature, duration and other parameters of the program are highly intelligent, can be automatically adjusted, and are easy to use.

Third, the integrated equipment reduces the space occupied by multiple equipment, simplifies the number of equipment that operators need to operate, reduces the workload of manpower, and is suitable for popularization and application.

The present invention will be further described in detail below with reference to the accompanying drawings and examples; however, the integrated equipment for cultivating microorganisms of the present invention is not limited to the examples.

Description of drawings

Fig. 1 is a structural representation of the present invention;

Fig. 2 is a schematic structural view (side view) of the console device in the first state according to an embodiment of the present invention;

Fig. 3 is a schematic structural view (side view) of the console device in Fig. 2 in a second state;

Fig. 4 is a schematic structural view (side view) of the console device in the first state according to another embodiment of the present invention;

Fig. 5 is the front view of Fig. 4 console device;

Fig. 6 is a schematic structural view (side view) of the console device in Fig. 4 in a second state;

Fig. 7 is a schematic diagram of a circuit module of the present invention.

detailed description

Embodiment, see also shown in Fig. 1, a kind of microbial cultivation integrated equipment of the present invention comprises a box room 1, and the wall body of box room 1 is made of stainless steel plate and is provided with movable window, and this window is The push-pull glass window structure can be adjusted up and down to meet the height requirements of the operation without opening too much, so as not to damage the cleanliness of the interior of the chamber. The bottom of the chamber 1 is provided with a constant temperature air bath circulation generator 2 and an ozone generator 3 , and the top is provided with an air filter 4 and an ultraviolet lamp 5 . The constant temperature air bath circulation generator 2 is used to generate a constant temperature circulating airflow in the chamber 1 to maintain a temperature suitable for microbial cultivation; the air filter 4 is used to filter the constant temperature circulating airflow entering the chamber 1; the ozone generator 3 It is used to generate ozone, and the generated ozone enters the constant temperature circulating air flow to disinfect the chamber 1 with ozone; the ultraviolet lamp 5 is used to disinfect the chamber 1 with ultraviolet light. Through the above device, the chamber maintains an ultra-clean and constant temperature environment.

A console device 6 and an oscillating device 7 are arranged in the chamber. The console device 6 is movably arranged in the chamber 1 and is used for inoculation operation. The console device 6 is unfolded in a first state to form a platform suspended in the chamber 1, and is folded in the chamber in a second state. 1 side wall. The oscillating device 7 is used to drive the culture bottle inoculated with microorganisms to perform horizontal repeated motion.

In this embodiment, with reference to Fig. 2 to Fig. 4, the console device 6 includes an operating panel 61 and two movable frames 62, and one side of the operating panel 61 is rotatably connected to the side wall of the chamber 1 so that it can be moved relative to the chamber 1. The side walls are turned over; the two movable frames 62 are respectively hinged between the opposite ends of the operating panel 1 and the side walls of the chamber 1 . Specifically, the movable frame 62 includes two connecting rods that are rotatably connected. In the first state, the two connecting rods are rotated to 180° so that the operating panel 61 is horizontally unfolded and suspended above by the movable frame 62, that is, the operating panel 61 1. A right triangle structure is formed between the movable frame 62 and the side wall of the chamber, and the movable frame 62 is used as the hypotenuse of the right triangle. The operating panel 61 is expanded horizontally to form an operating table, so that equipment such as alcohol lamps, lighters, alcohol watering cans, inoculation loops, alcohol cotton balls, pipette guns and tip boxes required for inoculation can be placed, and the inoculation can be performed. After the inoculation is completed, the inoculation equipment is taken out, and the two connecting rods are rotated to overlap and fit on the side wall of the chamber 1 in the second state, so that the operation panel 61 can be folded vertically to fit the side wall of the chamber 1. The other side of the operating panel 61 and the side wall corresponding to the box chamber 1 are provided with matching buckles (not shown in the figure). The space in the box chamber 1 can be vacated.

In another embodiment, referring to Fig. 5 to Fig. 6, the console device 6 includes an operating panel 61 and two poles 63, and one side of the operating panel 61 is rotatably connected to the side wall of the chamber 1 so as to be able to rotate relative to the chamber. 1 side wall is turned over; two struts 63 are horizontally rotatable and hinged on the side wall of the box chamber 1 below the operation panel 61 . Lift the operating panel 61 in the first state, and rotate the two poles 63 to be perpendicular to the side wall of the box chamber. The operating panel 61 can be erected horizontally on the poles 63 and used to place the required equipment for inoculation and perform inoculation operations, preferably Yes, the two poles 63 can be respectively located at the two ends of the bottom of the operation panel 61 to increase the stability. In the second state, the two support rods 63 are rotated to fit the side wall of the box chamber, and the operating panel 61 naturally hangs down to fit the side wall of the box chamber and is retracted, so that the space in the box chamber 1 can be released.

The oscillating device 7 includes a carrying platform and a driving mechanism for driving the carrying platform to perform horizontal reciprocating motion. The carrying platform can be arranged under the operating plate 61 and is provided with a number of fixed seats 71 for placing culture bottles, and the culture bottles inoculated with microorganisms can be placed on the fixed seats 711 for vibration culture. Since the culture bottle has a certain height, the console device 6 is designed to be foldable, so that the space can be made, the height of the chamber 1 can be reduced, and the oscillation and follow-up operations can be facilitated. A number of through holes are opened on the carrying platform 71 to facilitate the passage of constant temperature circulating air.

The integrated equipment of the present invention also includes an operating system, which is used to control the operation of the constant temperature air bath circulation generator, air filter, ozone generator, ultraviolet lamp and oscillating device. The control system includes a main control module, a power supply module and an input module. The main control module receives the instructions input by the input module and controls the operation of the constant temperature air bath circulation generator, air filter, ozone generator, ultraviolet lamp and oscillating device accordingly; the power supply module is used for power supply. The main control module is a micro control unit 8, and the input module is a touch screen 9 for easy operation.

In one embodiment, a fluorescent lamp 10 is also provided in the chamber for lighting. The fluorescent lamp 10 is also connected and controlled by the control system.

Microbial cultivation using the above-mentioned equipment includes the following steps:

The first step is to prepare the culture medium in the culture bottle according to the process requirements, fill the pipette tip box with tips, and sterilize them by high pressure steam for later use;

The second step is to turn on the power of the integrated device and set parameters through the touch screen. The parameters include but are not limited to the temperature and duration of microbial cultivation, the speed of the oscillator, the disinfecting time of the ozone generator, and the time when the ultraviolet lamp is turned on.

The third step is to unfold the operation panel of the operation table device, and place the alcohol lamp, lighter, alcohol spray can, inoculation loop, alcohol cotton ball, pipette gun and tip box and other equipment required for inoculation on the operation panel.

The fourth step is to start the constant temperature air bath circulation generator through the control system to generate a constant temperature circulating air bath. Bacteria-free and dust-free; start the ozone generator, and the generated ozone enters the constant temperature air bath circulation system to kill the ozone in the chamber and its surroundings. At this time, the micro-control unit MCU starts the ozone disinfecting time, and when the ozone disinfecting time is reached, the ozone is turned off Generator, after 2-3 hours, proceed to the next step to ensure that the ozone is completely decomposed by itself.

The fifth step is to keep the constant temperature air bath circulation generator and the air filter in operation, so as to keep the sterilized chamber in a sterile and dust-free state. Push up the movable window to a height that meets the operation requirements, place the culture bottle containing the sterilized medium on the operation board, start the ultraviolet lamp for ultraviolet disinfection, turn off the ultraviolet lamp after the disinfection is completed, and turn on the fluorescent lamp for lighting .

The sixth step is to inoculate the bacterial solution into the culture bottle according to the requirements of the aseptic inoculation operation of microorganisms. After inoculation, take out the equipment for inoculation, put away the operation panel of the console device, place the culture bottle on the fixed seat of the oscillation device, and start the oscillation device.

Step 7: According to the growth characteristics of microorganisms, the cultivation is completed after the cultivation time is reached, the oscillating device is turned off sequentially through the operating system, the constant temperature air bath cycle generator is turned off, the air filter is turned off, the movable window is opened, and the culture bottle is taken out and then closed. Finally, turn on the ultraviolet light to kill the chamber.

The above-mentioned embodiment is only used to further illustrate a kind of microbial cultivation integrated equipment of the present invention, but the present invention is not limited to the embodiment, any simple modification, equivalent change and modification made to the above-mentioned embodiment according to the technical essence of the present invention , all fall within the scope of protection of the technical solution of the present invention.

Claims (10)

1. An integrated equipment for microbial cultivation, characterized in that it comprises: a box room, the box room is provided with a movable window; A constant temperature air bath circulation generator, arranged at the bottom of the chamber, is used to generate a constant temperature circulating airflow in the chamber to maintain a temperature suitable for microbial cultivation; An air filter, arranged on the top of the chamber, is used to filter the constant temperature circulating airflow entering the chamber; The ozone generator is arranged at the bottom of the chamber for generating ozone, and the generated ozone enters the constant temperature circulating air flow to disinfect the chamber by ozone; The ultraviolet lamp is arranged on the top of the chamber, and is used to disinfect the chamber by ultraviolet rays; The operating table device is movably arranged in the chamber and used for inoculation operations. The operating table device is deployed in a first state to form a platform suspended in the chamber, and is folded on the side wall of the chamber in a second state. ; An oscillating device is arranged in the chamber, and the oscillating device is provided with a number of fixed seats for placing culture bottles, so as to drive the culture bottles inoculated with microorganisms to move horizontally repeatedly; The control system is used to control the operation of the constant temperature air bath circulation generator, air filter, ozone generator, ultraviolet lamp and oscillating device; The use of the equipment for microbial cultivation includes the following steps: deploying the operating table device in the chamber and placing the equipment required for microbial inoculation; closing the movable window, and sequentially starting the constant temperature air bath circulation generator through the control system to generate constant temperature circulating air flow, the The air filter filters the constant temperature circulating airflow, and the ozone generator performs ozone disinfection; turn off the ozone generator, put in the culture bottle with the culture medium after 2-3 hours, close the movable window, and start the ultraviolet lamp for disinfection ; After the sterilization is completed, the inoculation is carried out in the box room, and the console device is put away after the inoculation, the inoculated culture bottle is placed on the fixed seat of the oscillation device, and the oscillation device is controlled by the control system to oscillate. 2. The integrated microbial cultivation equipment according to claim 1, characterized in that: the wall of the chamber is made of stainless steel plate, and the movable window is a push-pull glass window whose opening degree can be adjusted up and down . 3. The integrated equipment for microbial cultivation according to claim 1, characterized in that: the operating table device includes an operating panel and two movable frames, one side of the operating panel is rotatably connected to the side wall of the chamber, and the two movable frames Respectively hinged between the two ends of the opposite side of the operating panel and the side wall of the box chamber; the movable frame includes two connecting rods that are rotatably connected, and in the first state, the two connecting rods rotate to 180° so that the The operation panel is unfolded horizontally, and in the second state, the two connecting rods are rotated to overlap so that the operation panel is folded and attached to the side wall of the chamber. 4. The integrated equipment for microbial cultivation according to claim 3, characterized in that: the other side of the operation panel and the side wall of the corresponding chamber are provided with matching buckles, and when the operation panel is in the second state, it passes through the clips. The buckle is fixed to the side wall of the box chamber. 5. The microbial cultivation integrated equipment according to claim 1, characterized in that: the console device includes an operation panel and two poles, one side of the operation panel is rotatably connected to the side wall of the chamber, and the two poles It can be horizontally rotatably hinged on the side wall of the box chamber below the operating panel; in the first state, the two poles rotate to be perpendicular to the side wall of the box chamber and the operating panel is mounted on the poles; in the second state, the The two poles are rotated to fit the side wall of the chamber, and the operating panel hangs down to fit the side wall of the chamber. 6. The microbial cultivation integrated equipment according to claim 1, characterized in that: the oscillating device includes a carrying platform and a driving mechanism for driving the carrying platform to perform horizontal reciprocating motion, the fixing seat is arranged on the carrying platform, The driving mechanism is connected to and controlled by the control system. 7. The integrated microbial cultivation equipment according to claim 6, characterized in that: the carrying platform is provided with several through holes. 8. The integrated equipment for microbial cultivation according to claim 1, characterized in that: the control system includes a main control module, a power supply module and an input module; the main control module receives instructions input from the input module and controls the constant temperature air bath cycle accordingly The generator, air filter, ozone generator, ultraviolet lamp and oscillation device operate; the power module is used for power supply. 9. The integrated microorganism cultivation equipment according to claim 8, characterized in that: the main control module is a micro control unit, and the input module is a touch screen. 10. The integrated equipment for microbial cultivation according to claim 1, characterized in that: a fluorescent lamp is also arranged in the chamber.