CN209039502U - Animal and plant cell large-scale culture device - Google Patents
Animal and plant cell large-scale culture device Download PDFInfo
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- CN209039502U CN209039502U CN201821506111.XU CN201821506111U CN209039502U CN 209039502 U CN209039502 U CN 209039502U CN 201821506111 U CN201821506111 U CN 201821506111U CN 209039502 U CN209039502 U CN 209039502U
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- incubator
- water tank
- plant cells
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Abstract
The utility model discloses an animal and plant cell large-scale culture apparatus, including the incubator, holding water box is installed to the interior bottom of incubator, holding water box's upper portion is provided with the recess, the internally mounted of recess has the culture dish, the hole of permeating water with the inside intercommunication of holding water box is seted up to bottom one side of recess, the ultrasonic wave board that shakes is installed respectively to holding water box's inside both sides, the internally mounted of incubator has supersonic generator, and a side wall mounting of holding water box has the temperature sensor who is used for surveying the inside temperature of holding water box, the both sides of incubator communicate respectively has intake pipe and outlet duct, the internally mounted of intake pipe has dustproof filter screen and sterilamp. The utility model discloses an add holding water box, culture dish and supersonic generator's design, culture cell under the condition of carrying out the water-bath heating culture dish, and can promote cell proliferation through intermittent low-intensity ultrasonic wave to the cell stimulation.
Description
Technical field
The utility model relates to technical field of cell culture, specially a kind of animal and plant cells large-scale culture device.
Background technique
No matter for entire biotechnology, or for one of them biological cloning technology, cell culture is all
One essential process, the extensive clone of cell culture inherently cell.Cell culture technology can be thin by one
Born of the same parents become many cells that are simple unicellular or seldom breaking up by mass propgation, this is the essential link of clone technology.
A large amount of cell or its metabolite are obtained by cell culture.Because biological product is got from cell, it is possible to say
Cell culture technology is most crucial, most basic technology in biotechnology.Cell culture work be widely used in biology,
The every field such as medicine, new drug development become one of most important basic science.
The environment that existing cell culture technology needs is more harsh, not only needs to keep good gnotobasis, and also
Need certain temperature and air quality.Due to the limitation of environment, common cell culture apparatus be difficult can on a large scale into
Row culture, and in culture, the reproduction speed of cell is slower, is unable to satisfy, the demand of quick large-scale culture.Therefore, we
Need to propose a kind of animal and plant cells large-scale culture device.
Utility model content
The purpose of this utility model is to provide a kind of animal and plant cells large-scale culture devices, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the utility model provides the following technical solutions:
The interior bottom of a kind of animal and plant cells large-scale culture device, including incubator, the incubator is equipped with heat preservation
The top setting of water tank, the insulated water tank is fluted, and the inside of the groove is equipped with culture dish, the bottom one of the groove
Side offers the permeable hole being connected to insulated water tank inside, and the inside two sides of the insulated water tank are separately installed with ultrasonic wave vibration
Plate, the inside of the incubator are equipped with supersonic generator, and the supersonic generator passes through connecting line and ultrasonic wave vibration plate
It is electrically connected, the interior bottom of the insulated water tank is equipped with electric heating wire, and is equipped in the one side wall of insulated water tank for visiting
The temperature sensor of insulated water tank internal temperature is surveyed, the two sides of the incubator have been respectively communicated with air inlet pipe and an air outlet pipe, described
The inside of air inlet pipe is equipped with anti-dust filter mesh and ultraviolet germicidal lamp, and the inside of the escape pipe is equipped with air ejector fan.
Preferably, the top of the culture dish is fixedly connected with fixed ring, and the bottom of the fixed ring is fixedly connected with iron
Pi Huan, and the top of fixed ring is fixedly connected with handle, the upper outside of the groove is fixedly connected with annular magnet.
Preferably, the depth of the culture dish is less than the depth of groove, and the diameter of the fixed ring is greater than the diameter of groove.
Preferably, the inside of the air inlet pipe is fixedly connected with two groups of barn doors, and two groups of barn doors are separately positioned on purple
The two sides of outside line bactericidal lamp.
Preferably, low intensity pulsed ultrasound out, ultrasonic frequency 0.8- occur for the supersonic generator
1.2MHz, sound wave active strength are 0.1-0.7W/cm2, radiation pattern is pulsed.
Preferably, the inner top of the incubator is equipped with ozone generator.
Preferably, the top of the insulated water tank offers string holes and water filling port, and the side bottom connection of insulated water tank
There is drainpipe, water discharging valve is installed on the drainpipe.
Preferably, the outside of the incubator is equipped with temperature controller, the signal output end of the temperature sensor and temperature control
The signal input part of device is electrically connected, and the control output end of the temperature controller and the automatically controlled end of electric heating wire are electrically connected.
Preferably, resilient rubbber cushion is fixedly connected on the inner wall of the groove.
Compared with prior art, the utility model has the beneficial effects that the utility model passes through addition
1, the design of insulated water tank, culture dish and supersonic generator is trained in the case where carrying out heating water bath culture dish
Cell is supported, and cell Proliferation can be promoted by the low-intensity ultrasonic of discontinuity to cytositimulation;
2, by the design of ultraviolet germicidal lamp, anti-dust filter mesh and air ejector fan, so that the inner air of incubator
Circulation keeps good cell proliferation air quality, improves cell proliferation speed;
3, by the way that the design of groove is arranged on insulated water tank top, culture dish is heated convenient for allround fastness, and culture dish is just
In taking-up, while the cell being convenient in culture dish can receive the stimulation of ultrasonic wave, easy to use, be worth of widely use.
Detailed description of the invention
Fig. 1 is a kind of schematic diagram of internal structure of animal and plant cells large-scale culture device incubator of the utility model;
Fig. 2 is a kind of schematic diagram of internal structure of animal and plant cells large-scale culture device air inlet pipe of the utility model;
Fig. 3 is a kind of schematic diagram of internal structure of animal and plant cells large-scale culture device escape pipe of the utility model;
Fig. 4 is a kind of structural schematic diagram of animal and plant cells large-scale culture device insulated water tank of the utility model;
Fig. 5 is a kind of structural schematic diagram of animal and plant cells large-scale culture device culture dish of the utility model;
Fig. 6 is that a kind of temperature control of animal and plant cells large-scale culture device of the utility model is electrically connected block diagram.
In figure: 1 incubator, 2 ozone generators, 3 air inlet pipe, 4 supersonic generators, 5 temperature controllers, 6 temperature sensors, 7
Ultrasonic wave vibration plate, 8 electric heating wires, 9 permeable holes, 10 drainpipes, 11 water discharging valves, 12 escape pipes, 13 water filling ports, 14 handles, 15
Fixed ring, 17 iron sheet rings, 16 culture dishes, 18 annular magnets, 19 string holes, 20 connecting lines, 21 ultraviolet germicidal lamps, 22 dustproof filters
Net, 23 barn doors, 24 air ejector fans, 25 grooves, 26 insulated water tanks.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-6 is please referred to, the utility model provides a kind of technical solution:
The interior bottom of a kind of animal and plant cells large-scale culture device, including incubator 1, the incubator 1 is equipped with guarantor
Reservoir 26, the top setting fluted 25 of the insulated water tank 26, the inside of the groove 25 is equipped with culture dish 16, described
The bottom side of groove 25 offers the permeable hole 9 being connected to inside insulated water tank 26, the inside two sides of the insulated water tank 26
It is separately installed with ultrasonic wave vibration plate 7, the inside of the incubator 1 is equipped with supersonic generator 4, the supersonic generator 4
It is electrically connected by connecting line 20 and ultrasonic wave vibration plate 7, the interior bottom of the insulated water tank 26 is equipped with electric heating wire 8, and protects
Temperature sensor 6 for detecting 26 internal temperature of insulated water tank is installed in the one side wall of reservoir 26, the incubator 1
Two sides have been respectively communicated with air inlet pipe 3 and escape pipe 12, and the inside of the air inlet pipe 3 is equipped with anti-dust filter mesh 22 and ultraviolet light kills
The inside of bacterium lamp 21, the escape pipe 12 is equipped with air ejector fan 24.
Preferably, the top of the culture dish 16 is fixedly connected with fixed ring 15, the bottom of the fixed ring 15 is fixed to be connected
It is connected to iron sheet ring 17, and the top of fixed ring 15 is fixedly connected with handle 14, the upper outside of the groove 25 is fixedly connected with
Annular magnet 18.
By adopting the above technical scheme, it is convenient for the pick-and-place of culture dish 16 by handle 14, is fixed by annular magnet 18
Culture dish 16.
Preferably, the depth of the culture dish 16 is less than the depth of groove 25, the diameter of the fixed ring 15 is greater than groove
25 diameter.
By adopting the above technical scheme, culture dish 16 is easily installed in groove 25.
Preferably, the inside of the air inlet pipe 3 is fixedly connected with two groups of barn doors 23, and two groups of barn doors 23 are respectively set
In the two sides of ultraviolet germicidal lamp 21.
By adopting the above technical scheme, by the design of barn door 23, ultraviolet light is avoided to leak out, avoids influencing cell culture,
And it not will cause the ultraviolet radiation of staff.
Preferably, low intensity pulsed ultrasound out, ultrasonic frequency 0.8- occur for the supersonic generator 4
1.2MHz, sound wave active strength are 0.1-0.7W/cm2, radiation pattern is pulsed.
By adopting the above technical scheme, low-intensity ultrasonic can expand cell membrane, accelerate the proliferation of cell.
Preferably, the inner top of the incubator 1 is equipped with ozone generator 2.
By adopting the above technical scheme, by the design of ozone generator 2, it is convenient for before and after incubator 1 cultivates cell to training
It supports and carries out ozone sterilization inside case 1.
Preferably, the top of the insulated water tank 26 offers string holes 19 and water filling port 13, and the side of insulated water tank 26
Bottom is communicated with drainpipe 10, is equipped with water discharging valve 11 on the drainpipe 10.
By adopting the above technical scheme, convenient for keeping the skin wet or draining inside insulated water tank 26, while string holes 19 is convenient for heat preservation
The energization of 26 inside electrical part of water tank connects.
Preferably, the outside of the incubator 1 is equipped with temperature controller 5, the signal output end and temperature of the temperature sensor 6
The signal input part for controlling device 5 (its model SCT-9100) is electrically connected, the control output end of the temperature controller 5 and electric heating wire
8 automatically controlled end is electrically connected.
By adopting the above technical scheme, realize that the temperature of heating water bath controls by temperature controller 5, it is easy to use.
Preferably, being fixedly connected with resilient rubbber cushion on the inner wall of the groove 25.
By adopting the above technical scheme, when ultrasonic wave vibration plate 7 works, the caused available buffering of vibration prevents from cultivating
Ware 16 and the inner wall of groove 25 collide.
Working principle: when in use, the utility model passes through insulated water tank 26, culture dish 16 and supersonic generator 4
Design, cell is cultivated in the case where carrying out heating water bath culture dish 16, and can pass through the low-intensity ultrasonic pair of discontinuity
Cytositimulation (every 10-15 minutes, opens supersonic generator 4 and works 20-40 seconds), promotes cell Proliferation;Pass through ultraviolet light
The design of bactericidal lamp 21, anti-dust filter mesh 22 and air ejector fan 24 keeps good so that the inner air of incubator 1 circulates
Cell proliferation air quality, improve cell proliferation speed;By the way that the design of groove 25, culture are arranged on 26 top of insulated water tank
Ware 16 is heated convenient for allround fastness, and culture dish 16 is convenient for taking out, while can receive and surpassing convenient for the cell in culture dish 16
The stimulation of sound wave, it is easy to use, it is worth of widely use.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (9)
1. a kind of animal and plant cells large-scale culture device, including incubator (1), it is characterised in that: the incubator (1) it is interior
Bottom is equipped with insulated water tank (26), and fluted (25) are arranged in the top of the insulated water tank (26), the groove (25) it is interior
Portion is equipped with culture dish (16), and the bottom side of the groove (25) offers and the internal permeable hole being connected to of insulated water tank (26)
(9), the inside two sides of the insulated water tank (26) are separately installed with ultrasonic wave vibration plate (7), the inside installation of the incubator (1)
Have supersonic generator (4), the supersonic generator (4) is electrically connected by connecting line (20) and ultrasonic wave vibration plate (7), institute
The interior bottom for stating insulated water tank (26) is equipped with electric heating wire (8), and is equipped in the one side wall of insulated water tank (26) for visiting
The temperature sensor (6) of insulated water tank (26) internal temperature is surveyed, the two sides of the incubator (1) have been respectively communicated with air inlet pipe (3)
With escape pipe (12), the inside of the air inlet pipe (3) is equipped with anti-dust filter mesh (22) and ultraviolet germicidal lamp (21), it is described go out
The inside of tracheae (12) is equipped with air ejector fan (24).
2. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the culture dish
(16) top is fixedly connected with fixed ring (15), and the bottom of the fixed ring (15) is fixedly connected with iron sheet ring (17), and solid
The top for determining ring (15) is fixedly connected with handle (14), and the upper outside of the groove (25) is fixedly connected with annular magnet
(18)。
3. a kind of animal and plant cells large-scale culture device according to claim 2, it is characterised in that: the culture dish
(16) depth is less than the depth of groove (25), and the diameter of the fixed ring (15) is greater than the diameter of groove (25).
4. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the air inlet pipe
(3) inside is fixedly connected with two groups of barn doors (23), and two groups of barn doors (23) are separately positioned on ultraviolet germicidal lamp (21)
Two sides.
5. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the ultrasonic wave hair
Low intensity pulsed ultrasound out, ultrasonic frequency 0.8-1.2MHz occur for raw device (4), and sound wave active strength is 0.1-
0.7W/cm2, radiation pattern is pulsed.
6. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the incubator
(1) inner top is equipped with ozone generator (2).
7. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the insulated water tank
(26) top offers string holes (19) and water filling port (13), and a side bottom of insulated water tank (26) is communicated with drainpipe
(10), water discharging valve (11) are installed on the drainpipe (10).
8. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the incubator
(1) outside is equipped with temperature controller (5), the signal output end of the temperature sensor (6) and the signal input part of temperature controller (5)
It is electrically connected, the control output end of the temperature controller (5) and the automatically controlled end of electric heating wire (8) are electrically connected.
9. a kind of animal and plant cells large-scale culture device according to claim 1, it is characterised in that: the groove (25)
Inner wall on be fixedly connected with resilient rubbber cushion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821506111.XU CN209039502U (en) | 2018-09-14 | 2018-09-14 | Animal and plant cell large-scale culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821506111.XU CN209039502U (en) | 2018-09-14 | 2018-09-14 | Animal and plant cell large-scale culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209039502U true CN209039502U (en) | 2019-06-28 |
Family
ID=67033295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821506111.XU Active CN209039502U (en) | 2018-09-14 | 2018-09-14 | Animal and plant cell large-scale culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209039502U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115175709A (en) * | 2020-01-03 | 2022-10-11 | Uv合伙有限公司 | Sterilization system and method |
-
2018
- 2018-09-14 CN CN201821506111.XU patent/CN209039502U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115175709A (en) * | 2020-01-03 | 2022-10-11 | Uv合伙有限公司 | Sterilization system and method |
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