CN104862223A - Automatic microorganism culture device - Google Patents
Automatic microorganism culture device Download PDFInfo
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- CN104862223A CN104862223A CN201510238137.5A CN201510238137A CN104862223A CN 104862223 A CN104862223 A CN 104862223A CN 201510238137 A CN201510238137 A CN 201510238137A CN 104862223 A CN104862223 A CN 104862223A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention provides an automatic microorganism culture device, belongs to the technical field of biology and solves the problems of low microorganism culture automation and the like in the prior art. The automatic microorganism culture device comprises a worktable and a frame, a culture dish is arranged on the worktable, a first sliding rail and a second sliding rail are fixedly arranged between the worktable and the culture dish, a first slider and a second slider are arranged on the first sliding rail and the second sliding rail respectively, an oscillation structure is arranged on the worktable and comprises a driving motor, a cam and a connecting rod, a lifting cylinder is fixed onto the frame, a servo motor is fixed on a piston rod of the lifting cylinder, and fan blades are fixed on an output shaft of the servo motor. The culture dish is provided with a first opening, a filter membrane and a backflow structure are arranged at the first opening, and a centrifugal structure is arranged on the worktable. The automatic microorganism culture device can simulate a swing bed for automatically culturing microorganisms, and culture solution can be recycled.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of culture apparatus, particularly the automatization culture apparatus of a kind of microorganism.
Background technology
Microorganism comprises: bacterium, virus, fungi and some small-sized protobionts, and its individual small being difficult to detects by an unaided eye, close with human relation, relates generally to the numerous areas such as food, medicine, industrial or agricultural, environmental protection.And the one of microorganism being cultivated in biological culture, mainly in order to obtain the microorganism being conducive to human being's production, life.Meanwhile, due to veryer long in culturing process to microorganism, and be easily subject to the temperature of nutrient solution, the impact of concentration, whole process can expend too much human and material resources resource.
Owing to there is above-mentioned problem, through retrieval, as Chinese patent literature discloses the microbial cultivation device [patent No.: ZL201420503437.2; Authorization Notice No.: CN204022836U].This microbial cultivation device, comprise microbial cultivation device body and base, and the bracing frame be arranged on microbial cultivation device body, and the microorganism culturing vessel be arranged on microbial cultivation device body, and the controller be arranged on microbial cultivation device body, and be arranged on the intrinsic microorganism storage box of microbial cultivation device, dividing plate is provided with in microbial cultivation device body, dividing plate is provided with supporting seat, microorganism culturing vessel are arranged on supporting seat, bracing frame is provided with drive unit, the output terminal of drive unit is connected with shaft coupling, shaft coupling bottom is connected with rotating shaft, rotating shaft extends in microorganism culturing vessel, temperature sensor is provided with in microorganism culturing vessel, well heater is provided with in supporting seat.
But this microbial cultivation device mainly adopts the mode of temperature control to cultivate microorganism, whole process still can expend too much human and material resources resource and level of automation is low, for those skilled in the art, need to develop a kind of can automatization microorganism be cultivated, and the microbial cultivation device of human and material resources resource can be saved to a greater extent again.
Summary of the invention
The object of the invention is to there are the problems referred to above for existing technology, propose the automatization culture apparatus of a kind of microorganism, the automatization culture apparatus of this microorganism has can simulate shaking table and carry out automatization cultivation to microorganism, nutrient solution is carried out to the feature of recycling simultaneously.
Object of the present invention realizes by following technical proposal: the automatization culture apparatus of a kind of microorganism, the frame comprising worktable and arrange on the table, it is characterized in that, described worktable is provided with the culture dish with cavity, the top of described culture dish offers opening, slide rail one and slide rail two is also fixed with between described worktable and culture dish, described slide rail one and slide rail two slide respectively and is provided with slide block one and slide block two, the bottom surface of described culture dish is fixed on slide block one and slide block two, described worktable is also provided with the oscillating structure that culture dish can be driven reciprocating, described oscillating structure comprises drive-motor, cam and connecting rod, described drive-motor is horizontally fixed on worktable, and the length direction of the output shaft of drive-motor and described slide rail one and slide rail two is perpendicular, described cam is fixed on the output shaft of drive-motor, one end of described connecting rod is hinged on cam, the other end of connecting rod is hinged on described culture dish, described frame is vertically fixed with lift cylinder, and the piston rod of described lift cylinder is towards the opening of described culture dish, and the piston rod end of lift cylinder is fixed with servomotor, and the output shaft of described servomotor straight down and be fixed with some flabellums, one sidewall of described culture dish offers opening one, described opening one place is provided with filtering membrane, and described opening one place is provided with the return-flow structure that can carry out recycle to nutrient solution, described worktable offers the groove of circular in cross-section, in described groove, be provided with the exocentric structure can collected the microorganism in culture dish.
The principle of work of the automatization culture apparatus of this microorganism is such: 1, servomotor drives the flabellum on output shaft to rotate, regulated the upper-lower position of flabellum by the lift cylinder in frame, thus the microorganism in culture dish and nutrient solution can be stirred; 2, because drive-motor can be with moving cam to rotate, by connecting rod hinged between cam and culture dish, and culture dish is fixed on slide block one and slide block two, thus culture dish is moved reciprocatingly on slide rail one and slide rail two, realize the shaking culture of simulation shaking-table; 3, by the return-flow structure on the filtering membrane at culture dish opening one place and worktable, thus can realize carrying out recycle to nutrient solution; 4, due to worktable being provided with exocentric structure, thus centrifugally to the microorganism in culture dish can be concentrated, and collected.
In the automatization culture apparatus of mentioned microorganism, described return-flow structure comprises the allotment ware be fixed in above-mentioned frame and the liquid pump one be fixed on above-mentioned worktable, described allotment ware is positioned at the top of above-mentioned culture dish, and a sidewall of described allotment ware offers fluid inlet and liquid outlet, pipe connecting one is provided with between described liquid pump one and described culture dish, one end of described pipe connecting one is connected with the opening one of described culture dish, the other end of described pipe connecting one is connected with the liquid feeding end of described liquid pump one, pipe connecting two is provided with between described allotment ware and described liquid pump one, one end of described pipe connecting two is connected with the outlet end of described liquid pump one, the other end of described pipe connecting two is connected with the fluid inlet of described allotment ware, the liquid outlet of described allotment ware is connected with return line, the other end of described return line is arranged in the opening of described culture dish.Nutrient solution can be separated with microorganism by the filtering membrane that culture dish opening one place is provided with; Simultaneously, liquid pump one is extracted isolating the nutrient solution after microorganism in culture dish by pipe connecting one, be transported in allotment ware by pipe connecting two, thus redistribution is carried out to the nutrient solution extracted, finally by return line deployed nutrient solution is back in culture dish and re-uses.
In the automatization culture apparatus of mentioned microorganism, described exocentric structure comprises the rotary electric machine be fixed in groove and the centrifugal drum with cavity, described rotary electric machine is vertically fixed on groove bottom wall, described centrifugal drum and described groove match, and described centrifugal drum is fixed on the output shaft of described rotary electric machine, the external diameter of described centrifugal drum is less than the internal diameter of described groove.Rotary electric machine drives centrifugal drum to rotate, thus realizes carrying out centrifugal being concentrated to the nutrient solution containing microorganism, and collects microorganism.
In the automatization culture apparatus of mentioned microorganism, described worktable is also fixed with liquid pump two, the liquid feeding end of described liquid pump two is connected by pipe connecting three with between above-mentioned centrifugal drum, and the outlet end of described liquid pump two is connected by pipe connecting four with between above-mentioned culture dish.Nutrient solution containing microorganism after carrying out centrifugal test in centrifugal drum is extracted by pipe connecting three by liquid pump two, is transported in culture dish, thus again cultivates by pipe connecting four.
In the automatization culture apparatus of mentioned microorganism, a sidewall of described culture dish offers opening two, described opening two is connected with drain pipe, the other end of described drain pipe is connected with above-mentioned centrifugal drum.The nutrient solution containing microorganism in culture dish is transported in centrifugal drum by drain pipe and carries out centrifugal being concentrated.
In the automatization culture apparatus of mentioned microorganism, a sidewall of described culture dish is fixed with well heater, described culture dish bottom wall of inner cavity is also provided with temperature sensor.By the temperature sensor in culture dish, thus can real-time monitored to the temperature conditions of nutrient solution, then by optimal temperature that well heater makes nutrient solution keep needed for microorganism.
In the automatization culture apparatus of mentioned microorganism, described pipe connecting one, pipe connecting three, return line and drain pipe are provided with magnetic valve.According to practical situation, the flow direction of nutrient solution can be automatically controlled by magnetic valve.
In the automatization culture apparatus of mentioned microorganism, described worktable is also provided with PLC, and above-mentioned drive-motor, lift cylinder, servomotor, well heater, temperature sensor and magnetic valve are all connected with described PLC by circuit.Whole microbial cultivation device can be realized by PLC and carry out automated operation.
Compared with prior art, the automatization culture apparatus of this microorganism has the following advantages:
1, drive-motor of the present invention can be with moving cam to rotate, and by connecting rod hinged between cam and culture dish, thus culture dish is moved reciprocatingly on slide rail one and slide rail two, realizes the shaking culture of simulation shaking-table.
2, the nutrient solution that liquid pump one of the present invention will isolate microorganism by pipe connecting one extracts, and is transported in allotment ware and carries out redistribution, to be back in culture dish by deployed nutrient solution to re-use finally by return line by pipe connecting two.
3, servomotor of the present invention drives flabellum to rotate, and the lift cylinder in frame can regulate the upper-lower position of flabellum, thus the microorganism in culture dish and nutrient solution can be stirred.
4, rotary electric machine of the present invention drives centrifugal drum to rotate, thus realizes being concentrated the microorganism in nutrient solution is centrifugal and collects.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 of the present inventionly faces structural representation;
Fig. 3 is left TV structure schematic diagram of the present invention;
Fig. 4 is broken section structural representation of the present invention;
Fig. 5 is the sectional structure schematic diagram of culture dish in the present invention.
In figure, 1, worktable; 2, frame; 3, culture dish; 4, slide rail one; 5, slide rail two; 6, slide block one; 7, slide block two; 8, drive-motor; 9, cam; 10, connecting rod; 11, lift cylinder; 12, servomotor; 13, flabellum; 14, filtering membrane; 15, ware is allocated; 16, liquid pump one; 17, pipe connecting one; 18, pipe connecting two; 19, return line; 20, rotary electric machine; 21, centrifugal drum; 22, liquid pump two; 23, pipe connecting three; 24, pipe connecting four; 25, drain pipe; 26, well heater; 27, temperature sensor; 28, magnetic valve.
Embodiment
Be below specific embodiments of the invention and by reference to the accompanying drawings, technical scheme of the present invention is further described, but the present invention be not limited to these embodiments.
As shown in Figure 1, Figure 2 and Figure 3, the automatization culture apparatus of a kind of microorganism, the frame 2 comprising worktable 1 and be arranged on worktable 1, worktable 1 is provided with the culture dish 3 with cavity, and the top of culture dish 3 offers opening.Frame 2 is vertically fixed with lift cylinder 11, and the piston rod of lift cylinder 11 is towards the opening of culture dish 3, and the piston rod end of lift cylinder 11 is fixed with servomotor 12, and the output shaft of servomotor 12 straight down and be fixed with four flabellums 13.
As shown in Figure 4, specifically, be also fixed with slide rail 1 and slide rail 25 between worktable 1 and culture dish 3, slide rail 1 and slide rail 25 slide respectively is provided with slide block 1 and slide block 27, and the bottom surface of culture dish 3 is fixed on slide block 1 and slide block 27.Simultaneously, worktable 1 is also provided with the oscillating structure that culture dish 3 can be driven reciprocating, oscillating structure comprises drive-motor 8, cam 9 and connecting rod 10, drive-motor 8 is horizontally fixed on worktable 1, and the length direction of the output shaft of drive-motor 8 and slide rail 1 and slide rail 25 is perpendicular, cam 9 is fixed on the output shaft of drive-motor 8, and one end of connecting rod 10 is hinged on cam 9, and the other end of connecting rod 10 is hinged on culture dish 3.Drive-motor 8 can be with moving cam 9 to rotate, and by connecting rod 10 hinged between cam 9 and culture dish 3, thus culture dish 3 can be moved reciprocatingly on slide rail 1 and slide rail 25, realizes the shaking culture of simulation shaking-table.
As shown in Figure 5, a sidewall of culture dish 3 offers opening one, opening one place is provided with filtering membrane 14, and opening one place is provided with the return-flow structure that can carry out recycle to nutrient solution.More specifically, return-flow structure comprises the allotment ware 15 be fixed in frame 2 and the liquid pump 1 be fixed on worktable 1, and allotment ware 15 is positioned at the top of culture dish 3, and a sidewall of allotment ware 15 offers fluid inlet and liquid outlet.Wherein, pipe connecting 1 is provided with between liquid pump 1 and culture dish 3, one end of pipe connecting 1 is connected with the opening one of culture dish 3, the other end of pipe connecting 1 is connected with the liquid feeding end of liquid pump 1, pipe connecting 2 18 is provided with between allotment ware 15 and liquid pump 1, one end of pipe connecting 2 18 is connected with the outlet end of liquid pump 1, the other end of pipe connecting 2 18 is connected with the fluid inlet of allotment ware 15, the liquid outlet of allotment ware 15 is connected with return line 19, and the other end of return line 19 is arranged in the opening of culture dish 3.Nutrient solution can be separated with microorganism by the filtering membrane 14 that culture dish 3 opening one place is provided with; Simultaneously, liquid pump 1 is extracted isolating the nutrient solution after microorganism in culture dish 3 by pipe connecting 1, be transported in allotment ware 15 by pipe connecting 2 18, thus redistribution is carried out to the nutrient solution extracted, finally by return line 19, deployed nutrient solution is back to re-using in culture dish 3.
Worktable 1 offers the groove of circular in cross-section, in groove, be provided with the exocentric structure can collected the microorganism in culture dish 3.More specifically, a sidewall of culture dish 3 offers opening two, opening two is connected with drain pipe 25, the other end of drain pipe 25 is connected with centrifugal drum 21.Exocentric structure comprises the rotary electric machine 20 be fixed in groove and the centrifugal drum 21 with cavity, rotary electric machine 20 is vertically fixed on groove bottom wall, centrifugal drum 21 and groove match, and centrifugal drum 21 is fixed on the output shaft of rotary electric machine 20, the external diameter of centrifugal drum 21 is less than the internal diameter of groove.Be transported in centrifugal drum 21 by the nutrient solution containing microorganism in culture dish 3 by drain pipe 25, rotary electric machine 20 drives centrifugal drum 21 to rotate, thus realizes carrying out centrifugal being concentrated to the nutrient solution containing microorganism, and collects microorganism.
Wherein, worktable 1 is also fixed with liquid pump 2 22, the liquid feeding end of liquid pump 2 22 is connected by pipe connecting 3 23 with between centrifugal drum 21, and the outlet end of liquid pump 2 22 is connected by pipe connecting 4 24 with between culture dish 3.Nutrient solution containing microorganism after carrying out centrifugal test in centrifugal drum 21 is extracted by pipe connecting 3 23 by liquid pump 2 22, is transported in culture dish 3, thus again cultivates by pipe connecting 4 24.
In addition, a sidewall of culture dish 3 is fixed with well heater 26, culture dish 3 bottom wall of inner cavity is also provided with temperature sensor 27.By the temperature sensor 27 in culture dish 3, thus can real-time monitored to the temperature conditions of nutrient solution, then by optimal temperature that well heater 26 makes nutrient solution keep needed for microorganism.And pipe connecting 1, pipe connecting 3 23, return line 19 and drain pipe 25 are provided with magnetic valve 28.According to practical situation, the flow direction of nutrient solution can be automatically controlled by magnetic valve 28.Meanwhile, worktable 1 is also provided with PLC, drive-motor 8, lift cylinder 11, servomotor 12, well heater 26, temperature sensor 27 are all connected with PLC by circuit with magnetic valve 28.Whole microbial cultivation device can be realized by PLC and carry out automated operation, thus more efficient, convenient.
Comprehensively above-mentioned, the principle of work of the automatization culture apparatus of this microorganism is such: 1, servomotor 12 drives the flabellum 13 on output shaft to rotate, regulated the upper-lower position of flabellum 13 by the lift cylinder 11 in frame 2, thus the microorganism in culture dish 3 and nutrient solution can be stirred; 2, because drive-motor 8 can be with moving cam 9 to rotate, by connecting rod 10 hinged between cam 9 and culture dish 3, and culture dish 3 is fixed on slide block 1 and slide block 27, thus culture dish 3 can be moved reciprocatingly on slide rail 1 and slide rail 25, realize the shaking culture of simulation shaking-table; 3, nutrient solution can be separated with microorganism by filtering membrane 14, and liquid pump 1 is extracted isolating the nutrient solution after microorganism in culture dish 3 by pipe connecting 1, be transported in allotment ware 15 by pipe connecting 2 18, thus redistribution is carried out to the nutrient solution extracted, finally by return line 19, deployed nutrient solution is back to re-using in culture dish 3; 4, the nutrient solution containing microorganism in culture dish 3 is transported in centrifugal drum 21 by drain pipe 25, rotary electric machine 20 drives centrifugal drum 21 to rotate, thus realize carrying out centrifugal being concentrated to the nutrient solution containing microorganism, and microorganism is collected, simultaneously, by pipe connecting 3 23, the nutrient solution containing microorganism after carrying out centrifugal test in centrifugal drum 21 is extracted by liquid pump 2 22, be transported in culture dish 3 by pipe connecting 4 24, thus again cultivate; 5, whole microbial cultivation device can be realized by PLC and carry out automated operation, thus more efficient, convenient.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment, but can't depart from spirit of the present invention or surmount the scope that appended claims defines.
Although more employ 1 herein, worktable; 2, frame; 3, culture dish; 4, slide rail one; 5, slide rail two; 6, slide block one; 7, slide block two; 8, drive-motor; 9, cam; 10, connecting rod; 11, lift cylinder; 12, servomotor; 13, flabellum; 14, filtering membrane; 15, ware is allocated; 16, liquid pump one; 17, pipe connecting one; 18, pipe connecting two; 19, return line; 20, rotary electric machine; 21, centrifugal drum; 22, liquid pump two; 23, pipe connecting three; 24, pipe connecting four; 25, drain pipe; 26, well heater; 27, temperature sensor; 28, the term such as magnetic valve, but do not get rid of the possibility using other term.These terms are used to be only used to describe and explain essence of the present invention more easily; The restriction that they are construed to any one additional is all contrary with spirit of the present invention.
Claims (8)
1. the automatization culture apparatus of a microorganism, the frame comprising worktable and arrange on the table, it is characterized in that, described worktable is provided with the culture dish with cavity, the top of described culture dish offers opening, slide rail one and slide rail two is also fixed with between described worktable and culture dish, described slide rail one and slide rail two slide respectively and is provided with slide block one and slide block two, the bottom surface of described culture dish is fixed on slide block one and slide block two, described worktable is also provided with the oscillating structure that culture dish can be driven reciprocating, described oscillating structure comprises drive-motor, cam and connecting rod, described drive-motor is horizontally fixed on worktable, and the length direction of the output shaft of drive-motor and described slide rail one and slide rail two is perpendicular, described cam is fixed on the output shaft of drive-motor, one end of described connecting rod is hinged on cam, the other end of connecting rod is hinged on described culture dish, described frame is vertically fixed with lift cylinder, and the piston rod of described lift cylinder is towards the opening of described culture dish, and the piston rod end of lift cylinder is fixed with servomotor, and the output shaft of described servomotor straight down and be fixed with some flabellums, one sidewall of described culture dish offers opening one, described opening one place is provided with filtering membrane, and described opening one place is provided with the return-flow structure that can carry out recycle to nutrient solution, described worktable offers the groove of circular in cross-section, in described groove, be provided with the exocentric structure can collected the microorganism in culture dish.
2. the automatization culture apparatus of microorganism according to claim 1, it is characterized in that, described return-flow structure comprises the allotment ware be fixed in above-mentioned frame and the liquid pump one be fixed on above-mentioned worktable, described allotment ware is positioned at the top of above-mentioned culture dish, and a sidewall of described allotment ware offers fluid inlet and liquid outlet, pipe connecting one is provided with between described liquid pump one and described culture dish, one end of described pipe connecting one is connected with the opening one of described culture dish, the other end of described pipe connecting one is connected with the liquid feeding end of described liquid pump one, pipe connecting two is provided with between described allotment ware and described liquid pump one, one end of described pipe connecting two is connected with the outlet end of described liquid pump one, the other end of described pipe connecting two is connected with the fluid inlet of described allotment ware, the liquid outlet of described allotment ware is connected with return line, the other end of described return line is arranged in the opening of described culture dish.
3. the automatization culture apparatus of microorganism according to claim 2, it is characterized in that, described exocentric structure comprises the rotary electric machine be fixed in groove and the centrifugal drum with cavity, described rotary electric machine is vertically fixed on groove bottom wall, described centrifugal drum and described groove match, and described centrifugal drum is fixed on the output shaft of described rotary electric machine, the external diameter of described centrifugal drum is less than the internal diameter of described groove.
4. the automatization culture apparatus of microorganism according to claim 3, it is characterized in that, described worktable is also fixed with liquid pump two, the liquid feeding end of described liquid pump two is connected by pipe connecting three with between above-mentioned centrifugal drum, and the outlet end of described liquid pump two is connected by pipe connecting four with between above-mentioned culture dish.
5. the automatization culture apparatus of microorganism according to claim 4, it is characterized in that, one sidewall of described culture dish offers opening two, described opening two is connected with drain pipe, the other end of described drain pipe is connected with above-mentioned centrifugal drum.
6. the automatization culture apparatus of microorganism according to claim 5, is characterized in that, a sidewall of described culture dish is fixed with well heater, and described culture dish bottom wall of inner cavity is also provided with temperature sensor.
7. the automatization culture apparatus of microorganism according to claim 6, is characterized in that, described pipe connecting one, pipe connecting three, return line and drain pipe are provided with magnetic valve.
8. the automatization culture apparatus of microorganism according to claim 7, it is characterized in that, described worktable is also provided with PLC, and above-mentioned drive-motor, lift cylinder, servomotor, well heater, temperature sensor and magnetic valve are all connected with described PLC by circuit.
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