CN111394228A - Energy-saving and environment-friendly medical bacteria shaking culture system and method - Google Patents
Energy-saving and environment-friendly medical bacteria shaking culture system and method Download PDFInfo
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- CN111394228A CN111394228A CN202010272536.4A CN202010272536A CN111394228A CN 111394228 A CN111394228 A CN 111394228A CN 202010272536 A CN202010272536 A CN 202010272536A CN 111394228 A CN111394228 A CN 111394228A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims description 8
- 238000003756 stirring Methods 0.000 claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
- 230000010355 oscillation Effects 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 230000036544 posture Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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Abstract
The invention relates to an energy-saving environment-friendly medical bacteria shaking culture system which comprises an incubator (200), a culture dish (300) and a shaking and stirring part (400), wherein a first gear (403) driven by a motor (401) drives a stirring rod (407) to reciprocate relative to the culture dish (300), and a second gear (414) meshed with the first gear (403) drives the culture dish (300) to rotate along the circumferential direction so as to shake culture solution. According to the invention, the vertical movement of the stirring rod (407) is realized by the oscillation stirring part (400) through a single motor (401) only, so as to ensure the uniform concentration of the culture solution; and ensure the culture solution and the culture gas to be fully mixed; and the vertical posture is kept, so that the culture solution is not easy to spill, the first motor (401) is multipurpose, and the cost for arranging a plurality of motors (401) and controlling the motors (401) in a linkage manner is saved.
Description
Technical Field
The invention relates to an experimental prop, in particular to a system for medicine bacteria time.
Background
Shaking culture, also known as shake flask culture. It is a culture method in which microbial cells are inoculated in a liquid medium and placed on a shaker or a shaker with constant shaking. The oscillation can make the culture medium fully contact with oxygen, improve the supply of dissolved oxygen, the thallus after the oscillation culture is propagated uniformly, the culture efficiency is high, and the method is widely applied to strain screening and microorganism amplification culture and is a common culture mode in the fields of microorganism physiology, biochemistry, fermentation and other life science researches.
In general, shaking culture is that an experimenter shakes a culture dish by hands to realize the mixing of a culture solution and a culture gas, and the wave of the culture solution is increased to increase the activity of cells and avoid agglomeration. However, in many cases of high oxygen, low oxygen, high temperature, high pressure, low temperature, low pressure, etc., experimenters are inconveniently involved.
Therefore, there is a need for an energy-saving and environment-friendly medical bacteria shaking culture system capable of automatically shaking and stirring the culture solution in the culture dish.
Disclosure of Invention
The invention aims to provide an energy-saving and environment-friendly medical bacteria shaking culture system which can automatically shake and stir culture solution in a culture dish.
The invention relates to an energy-saving and environment-friendly medical bacteria shaking culture system, which comprises
The incubator is fixed with the supporting plate, and culture gas is arranged in the incubator;
a culture dish in which a culture solution is placed;
and the oscillating and stirring part drives the stirring rod to reciprocate relative to the culture dish by a first gear driven by a motor, and drives the culture dish to rotate along the circumferential direction by a second gear meshed with the first gear so as to oscillate the culture solution, and then the culture dish and the stirring rod are kept in vertical postures by a guide rod and a guide cylinder connected with the stirring rod and the culture dish.
The invention relates to an energy-saving and environment-friendly medical bacteria oscillation culture system, wherein an oscillation stirring part comprises a motor, an output shaft, a first gear, a first shaft, a first pin shaft, a first supporting rod, a stirring rod, a first connecting rod, a guide cylinder, a guide rod, a second connecting rod, a second supporting rod, a second pin shaft, a second gear and a second shaft, the output shaft of the motor drives the first shaft to rotate so as to enable the first gear coaxially fixed with the first shaft to rotate, the first pin shaft is arranged on the side surface of the first gear through a bearing, the first pin shaft is fixed with one end of the first supporting rod, the other end of the first supporting rod is fixed with the upper end of the stirring rod, the side surface of the first supporting rod is fixed with one end of the first connecting rod, the other end of the first connecting rod is fixed with the guide cylinder, the guide rod moving along the guide rod is arranged in the guide cylinder, the lower extreme of guide arm with the one end of second connecting rod is fixed, the other end of second connecting rod with the side surface of second bracing piece is fixed, the one end of second bracing piece with the side surface of culture dish is fixed, the other end of second bracing piece with second round pin hub fixation, the second round pin axle is installed through the bearing the side surface of second gear, the coaxial fixed second axle of second gear passes through the bearing and installs in the backup pad, second gear and first gear engagement, the stirring rod is just right directly over the culture dish.
The invention relates to an energy-saving and environment-friendly medical bacteria shaking culture system, wherein a motor is fixed with a support plate, an output shaft of the motor is coaxially fixed with a first shaft, and the side surface of the support plate and the side surface of an incubator are integrally fixed.
The invention relates to an energy-saving and environment-friendly medical bacteria shaking culture system, wherein an output shaft of a motor is fixed with the inner surface of an incubator, the output shaft of the motor passes through a first through hole formed in a support plate and is coaxially fixed with a first shaft, and the support plate is fixed with the bottom of the incubator.
The invention relates to an energy-saving and environment-friendly medical bacteria shaking culture system, wherein the upper part of a culture dish is communicated with one end of a water inlet pipe, the water inlet pipe is communicated with a water outlet of a water pump, a water inlet of the water pump is communicated with a water outlet pipe, and the water outlet pipe is communicated with the bottom of the culture dish.
The invention relates to an energy-saving and environment-friendly medical bacteria shaking culture system, wherein a stirring rod is a straight rod.
The invention relates to an energy-saving and environment-friendly medical bacteria shake culture system, wherein a stirring rod is a spiral rod body.
The invention relates to an energy-saving and environment-friendly medical bacteria shake culture system, wherein a stirring rod is a wavy rod body.
The invention relates to a method of an energy-saving and environment-friendly medical bacteria shaking culture system, which comprises the following steps:
step 1, introducing culture gas into an incubator, and keeping the concentration of the culture gas;
step 2, placing bacteria to be cultured in a culture dish;
step 3, electrifying the water pump to circulate the culture solution in the culture dish;
step 4, electrifying the motor for a preset time;
step 5, powering off the motor;
step 6, the water pump is powered off;
and 7, taking out the bacteria in the culture dish for observation.
The energy-saving and environment-friendly medical bacteria shaking culture system is different from the prior art in that the energy-saving and environment-friendly medical bacteria shaking culture system realizes the vertical movement of the stirring rod through the shaking stirring part only through a single motor so as to ensure the uniform concentration of a culture solution; the culture dish moves circumferentially to ensure the culture solution and the culture gas to be fully mixed; and the vertical posture is kept, so that the culture solution is not easy to spill, the first motor is multipurpose, and the cost for arranging a plurality of motors and performing linkage control on the motors is saved.
The energy-saving and environment-friendly medical bacteria shaking culture system is further described with reference to the accompanying drawings.
Drawings
FIG. 1 is a front view of an energy-saving and environment-friendly medical bacteria shaking culture system;
FIG. 2 is a dynamic variation diagram of FIG. 1;
FIG. 3 is a top view of FIG. 1;
fig. 4 is a top view of fig. 2.
Detailed Description
As shown in figures 1-4, referring to figure 1, the energy-saving and environment-friendly medical bacteria shaking culture system comprises
An incubator 200 fixed to the support plate 201 and having a culture gas provided therein;
a culture dish 300 in which a culture solution is placed;
and a shaking and stirring part 400 which reciprocates the stirring rod 407 relative to the culture dish 300 by a first gear 403 driven by a motor 401, shakes the culture solution by rotating the culture dish 300 in a circumferential direction by a second gear 414 engaged with the first gear 403, and maintains the culture dish 300 and the stirring rod 407 in a vertical posture by a guide rod 410 and a guide sleeve 409 connected to the stirring rod 407 and the culture dish 300.
According to the invention, the vertical movement of the stirring rod 407 is realized by the oscillation stirring part 400 through only a single motor 401, so as to ensure the uniform concentration of the culture solution; and the culture dish 300 moves circumferentially to ensure the culture solution and the culture gas to be fully mixed; and the vertical posture is kept, so that the culture solution is not easy to spill, the first motor 401 is multipurpose, and the cost for arranging a plurality of motors 401 and controlling the motors 401 in a linkage manner is saved.
That is, the agitation portion 400 ensures that the motion posture of the agitating bar 407 with respect to the culture dish 300 is only vertical motion without lateral component motion, thereby preventing the culture solution from spilling and ensuring the concentration of the culture solution at any position in the culture dish 300 to be uniform. Moreover, the culture dish 300 simulates the shaking of the culture dish 300 by a human hand along with the rotation of the second gear 414, so that the culture solution is fully mixed with the culture gas, the concentration of the culture gas in the culture solution is improved, and in addition, the culture dish 300 and the stirring rod 407 are kept in a vertical posture through the action of the guide rod 410 and the guide cylinder 409, so that the culture solution is prevented from being spilled out of the inclined culture dish 300, or the culture solution is stirred out by the stirring rod 407 with transverse component motion, and therefore, the culture solution is saved, the cost of the plurality of motors 401 is saved, the cost of the linkage control of the plurality of motors 401 is saved, and the power consumption of the plurality of motors 401 is saved.
Wherein the culture gas includes, but is not limited to: low oxygen low pressure gas, high oxygen high pressure gas.
Wherein, the culture solution can be: normal saline or nutrient solution.
The supporting plate 201 may be a plate or a rod, or a plate or a rod integrated with the side surface of the incubator 200.
As a further explanation of the present embodiment, referring to fig. 1 and 3, the oscillating stirring portion 400 includes a motor 401, an output shaft 402, a first gear 403, a first shaft 404, a first pin 405, a first support 406, a stirring rod 407, a first link 408, a guide tube 409, a guide rod 410, a second link 411, a second support 412, a second pin 413, a second gear 414, and a second shaft 415, the output shaft 402 of the motor 401 drives the first shaft 404 to rotate, so that the first gear 403 fixed coaxially with the first shaft 404 rotates, a side surface of the first gear 403 is mounted with the first pin 405 through a bearing, the first pin 405 is fixed to one end of the first support 406, the other end of the first support 406 is fixed to an upper end of the stirring rod 407, a side surface of the first support 406 is fixed to one end of the first link 408, and the other end of the first link 408 is fixed to the guide tube 409, a guide rod 410 moving along the guide cylinder 409 is arranged in the guide cylinder 409, the lower end of the guide rod 410 is fixed with one end of a second connecting rod 411, the other end of the second connecting rod 411 is fixed with the side surface of a second supporting rod 412, one end of the second supporting rod 412 is fixed with the side surface of the culture dish 300, the other end of the second supporting rod 412 is fixed with a second pin 413, the second pin 413 is installed on the side surface of a second gear 414 through a bearing, a second shaft 415 coaxially fixed with the second gear 414 is installed on the supporting plate 201 through a bearing, the second gear 414 is meshed with the first gear 403, and the stirring rod 407 is right above the culture dish 300.
In the present invention, the first gear 403 and the second gear 414 are simultaneously driven and rotated by the motor 401, so that the stirring rod 407 is driven to rotate in a circular motion by the first gear 403, and the culture solution is stirred by moving in the vertical direction of the culture dish 300 by the relative reverse rotation of the first gear 403 and the second gear 414; and the culture dish 300 is driven to rotate by the second gear 414 to simulate the operation of shaking culture of the culture solution by human hands, so that the culture solution is mixed with the culture gas by the shaking culture to increase the concentration of the culture gas in the culture solution; and the culture dish 300 and the stirring rod 407 do not move relatively in the left-right direction but only move relatively in the vertical direction through the guide rod 410 and the guide cylinder 409 which are associated with the first gear 403 and the second gear 414, so that the stirring rod 407 can not only fully stir the culture solution, but also prevent the culture solution from being stirred out of the culture dish 300 by the stirring rod 407 moving relatively in the lateral direction, and therefore the culture solution is saved, the energy is saved, and the cost of a plurality of motors 401 is saved.
The second gear 414 is disposed below the first gear 403.
The first support rod 406 is perpendicular to the stirring rod 407 and the first connecting rod 408, that is, as shown in fig. 1, the first support rod 406 may be disposed along the Z-axis direction, the first connecting rod 408 may be disposed along the X-axis direction, and the stirring rod 407 may be disposed along the Y-axis direction.
As a further explanation of the present embodiment, referring to fig. 2 and 4, the motor 401 is fixed to the support plate 201, the output shaft 402 of the motor 401 is coaxially fixed to the first shaft 404, and the side surface of the support plate 201 is integrally fixed to the side surface of the incubator 200.
In the present invention, the motor 401 can be directly fixed to the supporting plate 201 and engaged with the first gear 403 through the third gear 429 to drive the first gear 403 to rotate, so that the supporting plate 201 can be integrated with the side surface of the incubator 200, and even the side surface of the incubator 200 is used as the supporting plate 201, the process for arranging the supporting plate 201 can be reduced as much as possible, and the cost of parts can be reduced.
Of course, a variation of this embodiment may also be that, referring to fig. 1 and 3, the output shaft 402 of the motor 401 is fixed to the inner surface of the incubator 200, the output shaft 402 of the motor 401 passes through the first through hole 420 formed in the support plate 201 and is coaxially fixed to the first shaft 404, and the support plate 201 is fixed to the bottom of the incubator 200.
According to the invention, the motor 401 is isolated from the movable first support rod 406, the movable second support rod 412, the guide cylinder 409, the guide rod 410, the culture dish 300 and the stirring rod 407 by the support plate 201, so that the phenomenon that the normal operation of the motor 401 is affected by the culture solution spilled in the culture dish 300 wetting the motor 401 is avoided. Furthermore, the power module of the motor 401 should be disposed at the same side of the incubator 200 as the motor 401, rather than being wetted.
The motor 401 can also be connected with a power module outside the incubator 200 through a wire, of course, the power module can also be configured on one side of the incubator 200, which is far away from the culture dish 300 and is separated by the support plate 201, the power module can be a battery with a circuit board, the power module is connected with the motor 401 through a wire, and the power module is connected with a user terminal through a wireless communication module to adjust the rotating speed and the rotating direction of the motor 401.
As a further explanation of the present embodiment, referring to fig. 1 and 3, the upper portion of the culture dish 300 is communicated with one end of a water inlet pipe 432, the water inlet pipe 432 is communicated with a water outlet of a water pump 431, a water inlet of the water pump 431 is communicated with a water outlet pipe 433, and the water outlet pipe 433 is communicated with the bottom of the culture dish 300.
In the present invention, the water pump 431 is used to continuously circulate the culture medium in the culture dish 300, so that the culture medium in the culture dish 300 can be used to continue culturing bacteria through the circulated culture medium after being consumed by bacteria, thereby providing nutrition. The water pump 431 may be turned off at variable times and the water inlet connected to the water outlet pipe 432 may be turned off to ensure that the culture dish 300 is filled with the culture solution, and the culture solution may be continuously mixed with the culture gas.
In the invention, the water pump 431 can be arranged in the water tank, the water outlet pipe 432, the water inlet pipe 432 and the water pump 431 are arranged in the incubator 200 or the water pump 431 is arranged outside the incubator 200, the water outlet pipe 432 and the water inlet pipe 432 pass through a first through hole formed in the incubator 200 to be communicated with the culture dish 300 in the incubator 200, and a sealing ring is arranged in the first through hole to ensure the sealing property.
As a further explanation of this embodiment, referring to fig. 1 and 3, the stirring rod 407 is a straight rod.
The present invention can prevent the culture solution from overflowing by the straight rod-shaped stirring rod 407, and thus can save the culture solution as much as possible.
Of course, a variant of this embodiment is that, referring to fig. 2, the stirring rod 407 is a helical rod body.
The present invention increases the uniformity of the culture solution and the chance of mixing the culture solution with the culture gas by the above-mentioned spiral stirring rod 407 to improve the stirring efficiency when it moves longitudinally.
Of course, a variation of this embodiment is that, referring to fig. 2, the stirring rod 407 is a wave-shaped rod body.
The present invention increases the stirring efficiency by the above-mentioned wavy stirring rod 407 in its longitudinal movement, and increases the uniformity of the culture solution and the chance of mixing the culture solution with the culture gas.
The invention relates to a method of an energy-saving and environment-friendly medical bacteria shaking culture system, which comprises the following steps:
step 1, introducing culture gas into an incubator 200, and maintaining the concentration of the culture gas;
step 2, placing bacteria to be cultured in the culture dish 300;
step 3, electrifying the water pump 431 to circulate the culture solution in the culture dish 300;
step 4, electrifying the motor 401 for a preset time;
step 5, the motor 401 is powered off;
step 6, the water pump 431 is powered off;
in step 7, the bacteria in the culture dish 300 are removed and observed.
The method ensures that the bacteria have a sufficient culture environment when being subjected to shaking culture, and avoids the influence on the culture condition caused by excessive contact with the outside.
The preset time in the present invention may be 2 minutes to 24 hours.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (9)
1. An energy-concerving and environment-protective medical bacterium shake culture system which characterized in that: comprises that
An incubator (200) fixed to the support plate (201) and containing culture gas therein;
a culture dish (300) in which a culture solution is placed;
and a shaking and stirring part (400) which drives the stirring rod (407) to reciprocate relative to the culture dish (300) by a first gear (403) driven by a motor (401), drives the culture dish (300) to rotate along the circumferential direction by a second gear (414) meshed with the first gear (403) to shake the culture solution, and keeps the culture dish (300) and the stirring rod (407) in a vertical posture by a guide rod (410) and a guide cylinder (409) connected with the stirring rod (407) and the culture dish (300).
2. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 1, characterized in that: the oscillating stirring part (400) comprises a motor (401), an output shaft (402), a first gear (403), a first shaft (404), a first pin shaft (405), a first support rod (406), a stirring rod (407), a first connecting rod (408), a guide cylinder (409), a guide rod (410), a second connecting rod (411), a second support rod (412), a second pin shaft (413), a second gear (414) and a second shaft (415), wherein the output shaft (402) of the motor (401) drives the first shaft (404) to rotate so as to enable the first gear (403) coaxially fixed with the first shaft (404) to rotate, the first pin shaft (405) is mounted on the side surface of the first gear (403) through a bearing, the first pin shaft (405) is fixed with one end of the first support rod (406), and the other end of the first support rod (406) is fixed with the upper end of the stirring rod (407), the side surface of the first supporting rod (406) is fixed with one end of the first connecting rod (408), the other end of the first connecting rod (408) is fixed with the guide cylinder (409), a guide rod (410) moving along the guide cylinder (409) is arranged in the guide cylinder (409), the lower end of the guide rod (410) is fixed with one end of the second connecting rod (411), the other end of the second connecting rod (411) is fixed with the side surface of the second supporting rod (412), one end of the second supporting rod (412) is fixed with the side surface of the culture dish (300), the other end of the second supporting rod (412) is fixed with the second pin shaft (413), the second pin shaft (413) is installed on the side surface of the second gear (414) through a bearing, a second shaft (415) coaxially fixed with the second gear (414) is installed on the supporting plate (201) through a bearing, and the second gear (414) is meshed with the first gear (403), the stirring rod (407) is right above the culture dish (300).
3. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 2, characterized in that: the motor (401) is fixed with the support plate (201), an output shaft (402) of the motor (401) is coaxially fixed with the first shaft (404), and the side surface of the support plate (201) is integrally fixed with the side surface of the incubator (200).
4. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 2, characterized in that: an output shaft (402) of the motor (401) is fixed with the inner surface of the incubator (200), the output shaft (402) of the motor (401) penetrates through a first through hole (420) formed in the supporting plate (201) to be coaxially fixed with the first shaft (404), and the supporting plate (201) is fixed with the bottom of the incubator (200).
5. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 4, characterized in that: the upper portion of the culture dish (300) is communicated with one end of a water inlet pipe (432), the water inlet pipe (432) is communicated with a water outlet of a water pump (431), a water inlet of the water pump (431) is communicated with a water outlet pipe (433), and the water outlet pipe (433) is communicated with the bottom of the culture dish (300).
6. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 5, characterized in that: the stirring rod (407) is a straight rod.
7. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 5, characterized in that: the stirring rod (407) is a spiral rod body.
8. The energy-saving environment-friendly medical bacteria shaking culture system according to claim 5, characterized in that: the stirring rod (407) is a wave-shaped rod body.
9. The method for the energy-saving and environment-friendly medical bacteria shaking culture system of claim 8, is characterized by comprising the following steps:
step 1, introducing culture gas into an incubator (200), and maintaining the concentration of the culture gas;
step 2, placing bacteria to be cultured in a culture dish (300);
step 3, electrifying the water pump (431) to circulate the culture solution in the culture dish (300);
step 4, electrifying the motor (401) for a preset time;
step 5, powering off the motor (401);
step 6, the water pump (431) is powered off;
step 7, the bacteria in the culture dish (300) are taken out and observed.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113019202A (en) * | 2021-03-03 | 2021-06-25 | 邓新 | Pediatric and gynecological test device and medicament brewing device |
| CN116769583A (en) * | 2021-05-21 | 2023-09-19 | 刘洋 | Full-automatic bacterial culture device |
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|---|---|---|---|---|
| EP0000512A1 (en) * | 1977-07-21 | 1979-02-07 | BASF Aktiengesellschaft | Self-supporting helical coil type stirrer |
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| CN113019202A (en) * | 2021-03-03 | 2021-06-25 | 邓新 | Pediatric and gynecological test device and medicament brewing device |
| CN116769583A (en) * | 2021-05-21 | 2023-09-19 | 刘洋 | Full-automatic bacterial culture device |
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Application publication date: 20200710 |