CN207243886U - A kind of biology shaking table - Google Patents
A kind of biology shaking table Download PDFInfo
- Publication number
- CN207243886U CN207243886U CN201721025882.2U CN201721025882U CN207243886U CN 207243886 U CN207243886 U CN 207243886U CN 201721025882 U CN201721025882 U CN 201721025882U CN 207243886 U CN207243886 U CN 207243886U
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- China
- Prior art keywords
- pallet
- rotating mechanism
- driving device
- biology
- shaking table
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- 238000004113 cell culture Methods 0.000 claims abstract description 28
- 230000033001 locomotion Effects 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims description 22
- 239000000523 sample Substances 0.000 claims description 21
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 abstract description 11
- 239000001963 growth medium Substances 0.000 abstract description 7
- 238000010008 shearing Methods 0.000 abstract description 5
- 230000010261 cell growth Effects 0.000 abstract description 4
- 239000006260 foam Substances 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
Abstract
A kind of biology shaking table, including：Housing, driving device, pallet, incubator and the rotating mechanism with angle of inclination and eccentric shaft；Driving device is installed in the housing, and the output terminal of driving device is connected to rotating mechanism, and pallet is installed on rotating mechanism, and incubator is arranged on pallet；Cell culture bags are positioned in pallet, and driving device driving rotating mechanism carries out 360 ° of rotations, and rotating mechanism drives pallet to be rotated in the range of 3d space, and pallet drives the cell culture bags in incubator to do corresponding 3D rotary motions.When being rotated due to driving rotating mechanism to carry out 360 ° by driving device, rotating mechanism drives pallet to be rotated in the range of 3d space, pallet drives the cell culture bags in incubator to do corresponding 3D rotary motions, the mixing of cell and culture medium can be made more uniform, it is more advantageous to the propagation growth of cell, compared with the shearing force that traditional zymotic jar has smaller, non-foam produces in cell cultivation process, improves cytoactive.
Description
Technical field
It the utility model is related to bio-medical technology field, and in particular to a kind of biology shaking table.
Background technology
The species of disposable bioreactor is very much, including membrane bioreactor, wave bioreactor, rock type life
Thing reactor, stirring type bioreactor, gas-powered formula reactor etc., its cell blending manner is different.Shaken with tradition
Exemplified by bottle and stirring type bioreactor, the effect for the shaking flask mixed-culture medium that it shakes back and forth horizontally or horizontal circumference shakes is not
It is fine, the dissolved oxygen content in nutrient solution is not very high.
The content of the invention
The application provides a kind of biological shaking table, including housing, driving device, pallet, incubator and with angle of inclination and
The rotating mechanism of eccentric shaft；
Driving device is installed in housing, and the output terminal of driving device is connected to rotating mechanism, and pallet is installed on whirler
Structure, incubator are arranged on pallet；
Cell culture bags are positioned in pallet, and driving device driving rotating mechanism carries out 360 ° of rotations, and then, rotating mechanism
Pallet is driven to be rotated in the range of 3d space, pallet drives the cell culture bags in incubator to do corresponding 3D rotations fortune
It is dynamic.
In a kind of embodiment, rotating mechanism includes：Swash plate, elastic component and Bearning mechanism；
Swash plate lower part is equipped with central channel, and swash plate top is equipped with the eccentric shaft of opposite central channel bias；
The output terminal of driving device is placed in central channel, and Bearning mechanism is socketed on eccentric shaft, and pallet connects with Bearning mechanism
Connect；
Elastic component is cylindrical, is sheathed on swash plate, and the bottom of elastic component is undertaken in housing upper surface, the upper end of elastic component
Portion is undertaken in below pallet.
In a kind of embodiment, the contact surface of swash plate and Bearning mechanism is inclined plane.
In a kind of embodiment, the bottom of elastic component is fixedly connected on housing upper surface.
In a kind of embodiment, driving device is motor.
In a kind of embodiment, elastic component is rubber ring.
In a kind of embodiment, temperature control equipment, temperature control equipment includes heating plate, temp probe and processor；
The center of pallet is equipped with the holding tank of upward opening, and the upper surface of pallet is respectively equipped with length in holding tank both sides
Strip-shaped clamp groove, heating plate are respectively arranged in strip card slot；
Temp probe is arranged in holding tank, and the top of temp probe at the top of heating plate with flushing；
The temperature for gathering cell culture bags is sent to processor by temp probe in real time, and processor is according to the temperature control of acquisition
The start and stop of heating plate processed.
In a kind of embodiment, temperature control equipment further includes resilient support portion, and resilient support portion is installed in holding tank, bullet
Property the bottom of supporting part and the bottom of holding tank connect, the top in resilient support portion is supported to temp probe.
In a kind of embodiment, temperature control equipment further includes man-machine interface, and man-machine interface exchanges data with processor.
The biological shaking table of foundation above-described embodiment, when being rotated due to driving rotating mechanism to carry out 360 ° by driving device,
Rotating mechanism drives pallet to be rotated under 3d space scope, and then, pallet drives the cell culture bags in incubator to do phase
The 3D rotary motions answered, can provide the mixing pattern of milder, make the mixing of cell and culture medium more uniform, are more advantageous to
The propagation growth of cell, compared with the shearing force that traditional zymotic jar has smaller, non-foam produces in cell cultivation process, improves cell
Activity；In addition, cell culture bags are heated and kept the temperature to it by temperature control equipment during doing 3D rotary motions, profit
In cell mass culture.
Brief description of the drawings
Fig. 1 is biological cradle structure schematic diagram；
Fig. 2 is rotating mechanism structure diagram；
Fig. 3 is oblique disk structure schematic diagram；
Fig. 4 is temperature control equipment scheme of installation；
Fig. 5 installs close-up schematic view for temperature control equipment；
Fig. 6 is temperature control equipment schematic diagram.
Embodiment
The utility model is described in further detail below by embodiment combination attached drawing.
In terms of bioreactor blending manner, according to the difference of mechanical structure, " seesaw " formula peace generally can be divided into
Face Clothoid type." seesaw " formula generally provides power by motor, passes through the band moving axis of the reciprocating rotary in certain angle of motor
Reciprocating rotation, axis will drive the pallet on stent to do seesaw type movement；Plane Clothoid type is also to provide power by motor,
The pallet on stent is driven planar to rotate by the rotation of motor.Both blending manners cannot make cell and training
It is more uniform to support base mixing, is unfavorable for absorption of the cell to nutriment and oxygen；Therefore, this example provides a kind of with 3D rotations
The biological shaking table of mixing pattern, its structure chart are as shown in Figure 1.
The biological shaking table of this example includes housing 1, driving device 2, pallet 3, incubator 4 and rotating mechanism 5, the driving of this example
Device 2 is preferably motor, and driving device 2 is installed in housing 1, and the output terminal of driving device 2 is connected to rotating mechanism 5, pallet 3
Rotating mechanism 5 is installed on, incubator 4 is arranged on pallet 3；Cell culture bags 6 are positioned in pallet 3, the driving rotation of driving device 2
Rotation mechanism 5 carries out 360 degree of rotations, and then, rotating mechanism 5 drives pallet 3 to be rotated in the range of 3d space, and pallet 3 drives
Corresponding 3D rotary motions are done in 6 bags of cell culture in incubator 4.
The pattern that this example is mixed using 3D rotations, one side cell and culture medium mixing are more uniform, can further expand
Cell improves the amount of dissolved oxygen in nutrient solution, while reduces cell shearing power, be the propagation of cell with the contact area of culture medium
Growth provides more preferable external environment condition.On the other hand stirring slurry end of blade and bubbling are avoided in stirred-tank fermenter to the wound of cell
Evil, there is provided gentle, the cell culture microenvironment of low-shearing force, high dissolved oxygen, be conducive to improve cell state, improve cell density and
Yield.In addition, rotary relative to " seesaw " formula and plane, the pattern advantage that 3D rotations mix is：It can provide warmer
The mixing pattern of sum, make that cell and culture medium mix more uniformly, be more advantageous to the propagation growth of cell.
Realize in the technical solution that shaking table 3D rotations mix, it is preferred that emphasis is the design of rotating mechanism 5, the rotating mechanism of this example
5 have certain angle of inclination and bias, specifically, as shown in Figures 2 and 3, the rotating mechanism 5 of this example includes swash plate 51, elasticity
Part 52 and Bearning mechanism 53,51 lower part of swash plate are equipped with central channel 511, and 51 top of swash plate is equipped with the inclined of the opposite bias of central channel 511
Mandrel 512；The output terminal of driving device 2 is placed in central channel 511, and Bearning mechanism 53 is socketed on eccentric shaft 512, wherein, tiltedly
Disk 51 and the contact surface of Bearning mechanism 53 are inclined plane, and pallet 3 is connected with Bearning mechanism 53；Elastic component 52 is cylindrical, is arranged
In swash plate 51, the bottom of elastic component 52 is undertaken in 1 upper surface of housing, and the upper end of elastic component 52 is undertaken in the lower section of pallet 3.
Driving device 2 is engaged with rotating mechanism 5 realizes that the mode that shaking table 3D rotations mix is：Driving device 2 drives band
There is the swash plate 51 of certain angle of inclination and eccentric shaft to carry out 360 ° rotation movement, swash plate 51 and Bearning mechanism 53 rotate against fortune
It is dynamic, Bearning mechanism 53 and pallet 3 by screw connection, elastic component 52 it is spacing under, pallet 3 in the range of certain 3d space into
Row rotary motion, the movement of pallet 3 then drive 1 inner cell culture bag 6 of incubator to do corresponding 3D rotary motions (velocity interval 2
~40rpm), it is finally reached the well-mixed purposes of cell culture fluid of cell culture bags 6.
The function of the elastic component 52 of this example is：When pallet 3 is rotated in the range of certain 3d space, elastic component 52
Its Sloped rotating is carried out spacing；Preferably, the elastic component 52 of this example is rubber ring, and, the bottom of elastic component 52, which is fixed, to be connected
It is connected to 1 upper surface of housing.
Further, carry out to realize cell culture bags 2 in the range of 3d space it is rotating during it is heated,
Insulation, this example further include temperature control equipment 7, temperature control equipment 7 include heating plate 71, temp probe 72, processor 73 plus
Hot plate controller 74, man-machine interface 75 and resilient support portion 76.
The scheme of installation of temperature control equipment 7 as shown in figure 4, partial enlarged view as shown in figure 5, schematic diagram such as Fig. 6 institutes
Show, the specific mounting means and operation principle of temperature control equipment 7 are：The center of pallet 3 is equipped with the appearance of upward opening
Receive groove, the upper surface of pallet 3 is respectively equipped with strip card slot in holding tank both sides, and heating plate 71 is respectively arranged at strip card slot
Interior, temp probe 72 is arranged in holding tank, and the top of temp probe 72 is flushed with the top of heating plate 71,；Cell culture bags 6
It is put into pallet 3, covers all heating plate 71, the heat of heating plate 71 is conducted to cell culture bags 6, at this time, temperature is visited
First 72 top is supported to cell culture bags 6, covers incubator 4, ensures that temperature is not lost in incubator 4, processor 73 is set
After heating-up temperature, processor 73 sends command signal to heating plate controller 74, and heating plate controller 74 is according to the command signal
Control heating plate 71 is begun to warm up, and during heating, temp probe 72 gathers the temperature of cell culture bags 6 in real time, and will adopt
The temperature of collection is sent to processor 73, and processor 73 judges the temperature value of reception compared with default temperature value, if sentencing
The temperature value to break to reception exceedes default temperature value, then processor 73 sends stop instruction signal to heating plate controller 74,
Heating plate controller 74 controls heating plate 71 to stop heating according to the stop instruction signal.
Further, the temperature parameter of demand is inputted by man-machine interface 75, man-machine interface 75 carries out data with processor 73
Exchange.
Further, the resilient support portion 76 of this example is installed in holding tank, the bottom in resilient support portion 76 and holding tank
Bottom connects, and the top in resilient support portion 76 is supported to temp probe 72, wherein, resilient support portion 76 includes the bullet with amount of elasticity
The purpose of spring, setting spring is that the height for the temp probe 72 for making its upper part fluctuates with 6 pressure size of cell culture bags, with
Ensure that temp probe 72 and cell culture bags 6 contact closely, can push up cell culture bags 6 if the height of temp probe 72 is too high
Rise, can so cause 6 inner cell shearing force of cell culture bags excessive and dead, if temp probe 72 is highly too low, so meeting
Temp probe 72 and cell culture bags 6 are caused to contact the reaction time for closely, not influencing heat conduction efficiency and temp probe 72.
This biological shaking table can not only meet 3D spinfunctions, and with heating and heat insulation function, can provide more
Gentle mixing pattern, make that cell and culture medium mix more uniformly, be more advantageous to the propagation growth of cell.
Use above specific case is illustrated the utility model, is only intended to help and understands the utility model, and
Not limiting the utility model.For those skilled in the art of the present invention, the think of according to the utility model
Think, some simple deductions, deformation can also be made or replaced.
Claims (9)
- A kind of 1. biology shaking table, it is characterised in that including：Housing, driving device, pallet, incubator and with angle of inclination and The rotating mechanism of eccentric shaft；The driving device is installed in the housing, and the output terminal of the driving device is connected to the rotating mechanism, described Pallet is installed on the rotating mechanism, and the incubator is arranged on the pallet；Cell culture bags are positioned in the pallet, and the driving device drives the rotating mechanism to carry out 360 ° of rotations, described Rotating mechanism drives the pallet to be rotated in the range of 3d space, and the pallet drives the cell culture in the incubator Bag does corresponding 3D rotary motions.
- 2. biology shaking table as claimed in claim 1, it is characterised in that the rotating mechanism includes：Swash plate, elastic component and bearing Mechanism；The swash plate lower part is equipped with central channel, and the swash plate top is equipped with the eccentric shaft of relatively described central channel bias；The output terminal of the driving device is placed in the central channel, and the Bearning mechanism is socketed on the eccentric shaft, described Pallet is connected with the Bearning mechanism；The elastic component is cylindrical, is sheathed on the swash plate, and the bottom of the elastic component is undertaken in the housing upper surface, The upper end of the elastic component is undertaken in below the pallet.
- 3. biology shaking table as claimed in claim 2, it is characterised in that the contact surface of the swash plate and the Bearning mechanism is to incline Inclined-plane.
- 4. biology shaking table as claimed in claim 2, it is characterised in that the bottom of the elastic component is fixedly connected on the shell Body upper surface.
- 5. biology shaking table as claimed in claim 2, it is characterised in that the driving device is motor.
- 6. biology shaking table as claimed in claim 2, it is characterised in that the elastic component is rubber ring.
- 7. biology shaking table as claimed in claim 1, it is characterised in that further include temperature control equipment, the temperature control dress Put including heating plate, temp probe and processor；The center of the pallet is equipped with the holding tank of upward opening, and the upper surface of the pallet is in the holding tank both sides point Not She You strip card slot, the heating plate is respectively arranged in the strip card slot；The temp probe is arranged in the holding tank, and the top of the temp probe at the top of heating plate with flushing；The temperature for gathering cell culture bags is sent to the processor by the temp probe in real time, and the processor is according to acquisition Temperature control described in heating plate start and stop.
- 8. biology shaking table as claimed in claim 7, it is characterised in that the temperature control equipment further includes resilient support portion, The resilient support portion is installed in the holding tank, and the bottom in the resilient support portion is connected with the bottom of the holding tank, The top in the resilient support portion is supported to the temp probe.
- 9. biology shaking table as claimed in claim 7, it is characterised in that the temperature control equipment further includes man-machine interface, institute State man-machine interface and exchange data with the processor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721025882.2U CN207243886U (en) | 2017-08-16 | 2017-08-16 | A kind of biology shaking table |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721025882.2U CN207243886U (en) | 2017-08-16 | 2017-08-16 | A kind of biology shaking table |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207243886U true CN207243886U (en) | 2018-04-17 |
Family
ID=61881429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721025882.2U Active CN207243886U (en) | 2017-08-16 | 2017-08-16 | A kind of biology shaking table |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207243886U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823094A (en) * | 2018-04-23 | 2018-11-16 | 宁波生动细胞科技有限公司 | cell culture system and method |
| CN113736655A (en) * | 2021-09-13 | 2021-12-03 | 衷博瑞佳生物技术(上海)有限公司 | Biological cell culture system |
-
2017
- 2017-08-16 CN CN201721025882.2U patent/CN207243886U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108823094A (en) * | 2018-04-23 | 2018-11-16 | 宁波生动细胞科技有限公司 | cell culture system and method |
| CN113736655A (en) * | 2021-09-13 | 2021-12-03 | 衷博瑞佳生物技术(上海)有限公司 | Biological cell culture system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190301 Address after: Room 301, Building C5, 555 Pioneer Road, Dayun Town, Jiashan County, Jiaxing City, Zhejiang Province Patentee after: Jiaxing Oufei Biotechnology Co.,Ltd. Address before: Room 802, 3388 Canghai Road, Yinzhou District, Ningbo City, Zhejiang Province Patentee before: NINGBO SHENGDONG CELL TECHNOLOGY CO.,LTD. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240118 Address after: Unit 330A, Building A2, No. 218 Xinghu Street, Industrial Park, Suzhou City, Jiangsu Province, 215000 Patentee after: Suzhou vivid Cell Biotechnology Co.,Ltd. Address before: Room 301, Building C5, 555 Pioneer Road, Dayun Town, Jiashan County, Jiaxing City, Zhejiang Province Patentee before: Jiaxing Oufei Biotechnology Co.,Ltd. |
|
| TR01 | Transfer of patent right |