CN111996111A

CN111996111A - Cell shaking incubator

Info

Publication number: CN111996111A
Application number: CN202010634835.8A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-03
Filing date: 2020-07-03
Publication date: 2020-11-27

Abstract

The invention discloses a cell shaking incubator, comprising: the device comprises a sterile box body, a controller, a sealing door, a constant temperature device and a culture mechanism; the controller is embedded in the right end of the front side of the sterile box body; the sealing door is hinged at the opening at the front side of the inner cavity of the sterile box body; the inner cavity of the constant temperature device is arranged on the left side of the sterile box body, the right side of the constant temperature device extends into the inner cavity of the sterile box body, and the constant temperature device is electrically connected with the controller; the culture mechanism is arranged at the bottom end of the inner cavity of the sterile box body. This cell shaking culture case when carrying out the shake culture to the culture dish, can adjust in time oscillation amplitude and oscillation direction as required, the suitability is strong to can realize the integration of shake culture and rotary cultivation, reduce staff's operation item, improve the device practicality.

Description

Cell shaking incubator

Technical Field

The invention relates to the technical field of cell engineering, in particular to a cell shaking incubator.

Background

Shaking culture, also called suspension culture, means culture in which cells of microorganisms or animals and plants are inoculated in a liquid medium and placed on a shaker to be shaken continuously, shaking enables the medium to be in full contact with oxygen, increases the supply of dissolved oxygen, and ensures uniform reproduction of thalli after shaking culture, high culture efficiency, wide application in strain screening and microorganism expansion culture, compared with static culture or surface culture of mold, and improving contact with medium components and oxygen supply during bacterial cell culture, uniform reproduction and high efficiency, especially for mold culture, no pellicle and pellet (pel-let) formed by growing hyphae can be formed, some varieties can form yeast-like morphology, and substance metabolism forms are similar to yeast, because the development and substance metabolism quantities are very large, therefore, agitation culture (still culture) having the same effect as shaking culture has been widely used in the microbial industry;

in the prior art, the cell shaking incubator can only carry out ascending rocking of horizontal direction to the cell culture dish to cultivate the cell in the culture dish, cell culture medium and oxygen contact effect are relatively poor, and the suspension culture method adopts different shaking culture equipment or rotatory cultivation equipment according to cultivateing the cell kind difference, and the operation is comparatively troublesome.

Disclosure of Invention

The invention aims to provide a cell shaking incubator, which at least solves the problems that the cell shaking incubator in the prior art can only shake a cell culture dish in the horizontal direction to culture cells in the culture dish, the contact effect of a cell culture medium and oxygen is poor, and the suspension culture method adopts different shaking culture equipment or rotating culture equipment according to different types of cultured cells, so that the operation is troublesome.

In order to achieve the purpose, the invention provides the following technical scheme: a cell shaking incubator comprising:

a sterile box body;

the controller is embedded in the right end of the front side of the sterile box body;

the sealing door is hinged at the opening at the front side of the inner cavity of the sterile box body;

the inner cavity of the constant temperature device is arranged on the left side of the sterile box body, the right side of the constant temperature device extends into the inner cavity of the sterile box body, and the constant temperature device is electrically connected with the controller;

and the culture mechanism is arranged at the bottom end of the inner cavity of the sterile box body.

Preferably, the culture mechanism comprises: the device comprises a shaking assembly, an oscillating assembly and a rotating assembly; the shaking assembly is embedded in the center of the top end of the inner cavity of the sterile box body; the oscillating assembly is arranged at the top end of the shaking assembly; the rotating assembly is mounted at the top end of the oscillating assembly.

Preferably, the wobble assembly comprises: the rocking assembly comprises a shell, a circular plate, an inner gear ring, a first motor and a gear; the shaking assembly shell is embedded in the center of the top end of the inner cavity of the sterile box body; the circular plate is rotatably connected to the upper surface of the shaking assembly shell through a bearing, an inner ring of the bearing is in interference fit with an outer wall of the circular plate, an outer ring of the bearing is fixedly connected with the inner wall of the shaking assembly shell, and the bottom end of the circular plate extends into an inner cavity of the shaking assembly shell; the inner gear ring is arranged on the lower surface of the circular plate; the first motor is arranged at the bottom end of the inner cavity of the shaking component shell along the up-down direction and is electrically connected with the controller; the gear screw is connected at the output end of the first motor, and the gear is meshed with the inner gear ring.

Preferably, the wobble assembly further comprises: the device comprises a limiting groove, a screw rod, a first gear, a second motor, a second gear, a screw rod nut and a supporting rod; the limiting groove is formed in the upper surface of the circular plate along the left-right direction; the screw rod is rotatably connected to the inner cavity of the limiting groove through a bearing along the left-right direction, an inner ring of the bearing is in interference fit with the outer wall of the screw rod, and an outer ring of the bearing is fixedly connected with the inner wall of the limiting groove; the first gear key is connected to the left side of the outer wall of the screw rod and the screw rod; the second motor is arranged in the middle of the top end of the circular plate along the left direction and is electrically connected with the controller; the second gear screw is connected to the output end of the second motor, and the second gear is meshed with the first gear; the screw nut is in threaded connection with the outer wall of the screw rod, and the outer wall of the screw nut is in adaptive insertion connection with the inner cavity of the limiting groove; the support rod is arranged at the top end of the screw nut along the up-down direction.

Preferably, the oscillating assembly includes: the bottom plate, the third motor, the cam and the limiting unit; the bottom plate is arranged at the top end of the supporting rod; the third motor is arranged at the center of the top end of the bottom plate through a bracket and is electrically connected with the controller; the cam screw is connected to the output end of the third motor; the number of the limiting units is four, and the four limiting units are arranged at four corners of the top end of the bottom plate respectively along the vertical direction.

Preferably, the limiting unit comprises: the limiting rod, the connecting rod and the limiting spring; the number of the limiting rods is four, the four limiting rods are respectively arranged at four corners of the top end of the bottom plate along the up-down direction, and the top end of an inner cavity of each limiting rod is communicated with the outer wall of the inner cavity of each limiting rod; the number of the connecting rods is four, and the four connecting rods are respectively inserted at the top end of the inner cavity of the limiting rod along the up-down direction; the limiting springs are arranged at the bottom ends of the inner cavities of the limiting rods in the vertical direction respectively, and the upper ends and the lower ends of the limiting springs are fixedly connected with the inner walls of the limiting rods and the bottom ends of the connecting rods respectively.

Preferably, the rotating assembly includes: the rotating assembly comprises a shell, a rotating plate, a rotating shaft, a worm wheel, a fourth motor and a worm; the rotating assembly shell is arranged at the top end of the limiting spring along the left-right direction, and the lower surface of the rotating assembly shell is in contact with the outer wall of the cam; the rotating plate is rotatably connected to the upper surface of the rotating assembly shell through a bearing, an inner ring of the bearing is in interference fit with the outer wall of the rotating plate, an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly shell, and the bottom end of the rotating plate extends into an inner cavity of the rotating assembly shell; the rotating shaft is rotatably connected to the bottom end of the inner cavity of the rotating assembly shell through a bearing in the vertical direction, an inner ring of the bearing is in interference fit with the outer wall of the rotating shaft, an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly shell, and the top end of the rotating shaft is fixedly connected with the lower surface of the rotating plate; the worm gear is in interference fit with the top end of the outer wall of the rotating shaft; the fourth motor is arranged at the bottom end of the inner cavity of the rotating assembly shell along the left-right direction and is electrically connected with the controller; and the worm is connected to the output end of the fourth motor along the left-right direction through screws, and the worm is meshed with the worm wheel.

Preferably, the rotating assembly further comprises: the fixing seat, the electric push rod and the arc-shaped clamping plate; the number of the fixed seats is two, and the two fixed seats are respectively arranged on the left side and the right side of the top end of the rotating plate; the number of the electric push rods is two, the two electric push rods are respectively arranged at the outer sides of the left fixing seat and the right fixing seat, the inner sides of the electric push rods extend into the inner sides of the fixing seats, and the electric push rods are electrically connected with the controller; the number of the arc-shaped clamping plates is two, and the two arc-shaped clamping plates are respectively arranged on the inner sides of the left electric push rod and the right electric push rod.

Compared with the prior art, the invention has the beneficial effects that: the cell oscillation incubator drives the gear to rotate through the first motor, so that the inner gear ring drives the circular plate to drive the culture dish to rotate circumferentially under the action of the gear rotating force, a culture medium in the culture dish is in full contact with oxygen, the second motor drives the second gear to rotate clockwise or anticlockwise, so that the first gear drives the screw rod to rotate anticlockwise or clockwise under the action of the second gear rotating force, the screw nut drives the support rod to move inwards or outwards under the action of the screw rod rotating force, the distance between the support rod and the axis of the circular plate is changed, and the horizontal oscillation amplitude of the cell culture dish can be changed; the cam is driven to rotate by the third motor, so that the far hub end and the near hub end of the outer wall of the cam are in intermittent contact with the lower surface of the rotating assembly shell, the rotating assembly shell is driven to intermittently reciprocate up and down to drive the culture dish to oscillate up and down, and different oscillation modes can be adopted as required to improve the sufficient contact area of the culture medium in the culture dish and oxygen; through the rotation of fourth motor drive worm, the worm wheel drives the pivot under the effect of worm rotatory power and drives the culture dish and rotate under the cooperation of rotor plate, electric push rod and arc pinch-off blades, and then carry out rotary culture to the cell in the culture dish, thereby when carrying out the shake culture to the culture dish, can adjust vibration amplitude and oscillation direction as required immediately, the suitability is strong, and can realize the integration of shake culture and rotary culture, reduce staff's operation item, improve the device practicality.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an exploded view of FIG. 1;

FIG. 3 is an exploded view of the culture mechanism of FIG. 2;

FIG. 4 is an exploded view of the spacing unit of FIG. 3;

FIG. 5 is an exploded view of the wobble assembly of FIG. 2;

fig. 6 is an exploded view of the rotating assembly of fig. 2.

In the figure: 1. the sterile box body, 2, a controller, 3, a sealing door, 4, a constant temperature device, 5, a culture mechanism, 6, a shaking component, 61, a shaking component shell, 62, a circular plate, 63, an internal gear ring, 64, a first motor, 65, a gear, 66, a limiting groove, 67, a screw rod, 68, a first gear, 69, a second motor, 610, a second gear, 611, a screw nut, 612, a supporting rod, 7, an oscillating component, 71, a bottom plate, 72, a third motor, 73, a cam, 74, a limiting unit, 741, a limiting rod, 742, a limiting spring, 743, a connecting rod, 8, a rotating component, 81, a rotating component shell, 82, a rotating plate, 83, a rotating shaft, 84, a worm wheel, 85, a fourth motor, 86, a worm, 87, a fixed seat, 88, an electric push rod, 89 and an arc-shaped clamping plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, the present invention provides a technical solution: a cell shaking incubator comprising: the device comprises an aseptic box body 1, a controller 2, a sealing door 3, a constant temperature device 4 and a culture mechanism 5, wherein the aseptic box body 1 ensures that the interior of the box body is in an aseptic environment; the controller 2 is embedded at the right end of the front side of the sterile box body 1, and the specific use model of the controller 2 is directly purchased, installed and used from the market according to the actual use requirement; the sealing door 3 is hinged at the opening at the front side of the inner cavity of the sterile box body 1; the inner cavity of the constant temperature device 4 is arranged on the left side of the sterile box body 1, the right side of the constant temperature device 4 extends into the inner cavity of the sterile box body 1, the constant temperature device 4 is electrically connected with the controller 2, the specific use model of the constant temperature device 4 is directly purchased, installed and used from the market according to the actual use requirement, and the temperature of the inner cavity of the sterile box body 1 can be adjusted by controlling the constant temperature device 4 through the controller 2 so that the cultured cells are at the constant temperature for growth; the culture mechanism 5 is arranged at the bottom end of the inner cavity of the sterile box body 1.

Preferably, the culture means 5 further comprises: a shaking assembly 6, an oscillating assembly 7 and a rotating assembly 8; the shaking component 6 is embedded in the center of the top end of the inner cavity of the sterile box body 1; the oscillating assembly 7 is arranged at the top end of the shaking assembly 6; the rotating assembly 8 is mounted on top of the oscillating assembly 7.

Preferably, the wobble assembly 6 further comprises: a wobble assembly housing 61, a circular plate 62, an inner gear ring 63, a first motor 64, and a gear 65; the shaking assembly shell 61 is embedded in the center of the top end of the inner cavity of the sterile box body 1; the circular plate 62 is rotatably connected to the upper surface of the shaking assembly housing 61 through a bearing, the inner ring of the bearing is in interference fit with the outer wall of the circular plate 62, the outer ring of the bearing is fixedly connected with the inner wall of the shaking assembly housing 61, and the bottom end of the circular plate 62 extends into the inner cavity of the shaking assembly housing 61; an inner gear ring 63 is provided on the lower surface of the circular plate 62; the first motor 64 is arranged at the bottom end of the inner cavity of the shaking assembly shell 61 in the vertical direction, the first motor 64 is electrically connected with the controller 2, the specific use model of the first motor 64 is directly purchased, installed and used from the market according to the actual use requirement, and the controller 2 controls the driving gear 65 to rotate clockwise for the first motor 64; the gear 65 is screwed on the output end of the first motor 64, the gear 65 is meshed with the internal gear ring 63, and the internal gear ring 63 can drive the circular plate 62 to rotate under the rotating force of the gear 65.

Preferably, the wobble assembly 6 further comprises: the limiting groove 66, the screw rod screw 67, the first gear 68, the second motor 69, the second gear 610, the screw nut 611 and the supporting rod 612; the limiting groove 66 is formed in the upper surface of the circular plate 62 in the left-right direction, and the limiting groove 66 can limit the screw nut 611 to prevent the screw nut 611 from rotating along with the screw rod 67; the screw rod screw 67 is rotatably connected to the inner cavity of the limiting groove 66 through a bearing along the left-right direction, the inner ring of the bearing is in interference fit with the outer wall of the screw rod screw 67, and the outer ring of the bearing is fixedly connected with the inner wall of the limiting groove 66; the first gear 68 is connected to the left side of the outer wall of the screw rod screw 67 in a key mode; the second motor 69 is arranged in the middle of the top end of the circular plate 62 along the left direction, the second motor 69 is electrically connected with the controller 2, the specific usage model of the second motor 69 is directly purchased and installed from the market and used according to the actual usage requirement, and the controller 2 controls the second motor 69 to drive the second gear 610 to rotate clockwise or anticlockwise; the second gear 610 is screwed at the output end of the second motor 69, the second gear 610 is meshed with the first gear 68, and the first gear 68 can drive the lead screw 67 to rotate counterclockwise or clockwise under the action of the rotating force of the second gear 610; the screw nut 611 is screwed on the outer wall of the screw rod screw 67, the outer wall of the screw nut 611 is in adaptive insertion with the inner cavity of the limiting groove 66, and the screw nut 611 can drive the support rod 612 to move inwards or outwards under the rotating force of the screw rod screw 67; the support rod 612 is disposed at the top end of the screw nut 611 in the up-down direction.

Preferably, the oscillating assembly 7 further comprises: a bottom plate 71, a third motor 72, a cam 73 and a limit unit 74; the bottom plate 71 is arranged at the top end of the supporting rod 612; the third motor 72 is installed at the center of the top end of the bottom plate 71 through a bracket, the third motor 72 is electrically connected with the controller 2, the specific use model of the third motor 72 is directly purchased and installed and used from the market according to the actual use requirement, and the controller 2 controls the third motor 72 to drive the cam 73 to rotate clockwise; the cam 73 is screwed at the output end of the third motor 72, and the distal hub end and the proximal hub end of the outer wall of the cam 73 are intermittently in contact with the lower surface of the rotating assembly housing 81; the number of the limiting units 74 is four, and the four limiting units 74 are respectively arranged at four corners of the top end of the bottom plate 71 along the vertical direction.

Preferably, the limiting unit 74 further includes: a limit rod 741, a connection rod 742, and a limit spring 743; the number of the limiting rods 741 is four, the four limiting rods 741 are respectively arranged at four corners of the top end of the bottom plate 71 in the vertical direction, and the top end and the outer wall of an inner cavity of each limiting rod 741 are communicated; the number of the connecting rods 742 is four, the four connecting rods 742 are respectively inserted into the top end of the inner cavity of the limiting rod 741 in the vertical direction, and the connecting rods 742 can move upwards or downwards in the inner cavity of the limiting rod 741 at corresponding positions, so that the rotating assembly housing 81 is limited and supported; the number of the limiting springs 743 is four, the four limiting springs 743 are respectively arranged at the bottom end of an inner cavity of the limiting rod 741 in the up-down direction, the upper end and the lower end of each limiting spring 743 are respectively fixedly connected with the inner wall of the limiting rod 741 and the bottom end of the connecting rod 742, each limiting spring 743 is a compression spring, the limiting springs 743 are stretched or extruded to generate elastic deformation, and after external force is removed, the springs are restored to the initial state.

Preferably, the rotating assembly 8 further comprises: a rotation assembly housing 81, a rotation plate 82, a rotation shaft 83, a worm wheel 84, a fourth motor 85, and a worm 86; the rotating block housing 81 is provided at the top end of the connecting rod 742 in the left-right direction, and the lower surface of the rotating block housing 81 is in contact with the outer wall of the cam 73; the rotating plate 82 is rotatably connected to the upper surface of the rotating assembly shell 81 through a bearing, an inner ring of the bearing is in interference fit with the outer wall of the rotating plate 82, an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly shell 81, and the bottom end of the rotating plate 82 extends into an inner cavity of the rotating assembly shell 81; the rotating shaft 83 is rotatably connected to the bottom end of the inner cavity of the rotating assembly housing 81 through a bearing in the up-down direction, an inner ring of the bearing is in interference fit with the outer wall of the rotating shaft 83, an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly housing 81, and the top end of the rotating shaft 83 is fixedly connected with the lower surface of the rotating plate 82; the worm gear 84 is in interference fit with the top end of the outer wall of the rotating shaft 83; the fourth motor 85 is arranged at the bottom end of the inner cavity of the rotating assembly shell 81 in the left-right direction, the fourth motor 85 is electrically connected with the controller 2, the specific usage model of the fourth motor 85 is directly purchased, installed and used from the market according to the actual usage requirement, and the controller 2 controls the fourth motor 85 to drive the worm 86 to rotate clockwise; the worm 86 is screwed to the output end of the fourth motor 85 in the left-right direction, the worm 86 is engaged with the worm wheel 84, and the worm wheel 84 can drive the rotation shaft 83 to rotate under the rotation force of the worm 86.

Preferably, the rotating assembly 8 further comprises: a fixed seat 87, an electric push rod 88 and an arc-shaped clamping plate 89; the number of the fixed seats 87 is two, and the two fixed seats 87 are respectively arranged at the left side and the right side of the top end of the rotating plate 82; the number of the electric push rods 88 is two, the two electric push rods 88 are respectively arranged at the outer sides of the left fixing seat 87 and the right fixing seat 87, the inner sides of the electric push rods 88 extend into the inner sides of the fixing seats 87, the electric push rods 88 are electrically connected with the controller 2, the electric push rods 88 are specifically purchased, installed and used from the market according to actual use requirements, and the electric push rods 88 are controlled by the controller 2 to be lengthened and shortened by themselves so as to push the arc-shaped clamping plates 89 at the corresponding positions to move inwards or outwards; the number of the arc-shaped clamping plates 89 is two, and the two arc-shaped clamping plates 89 are respectively arranged on the inner sides of the left electric push rod 88 and the right electric push rod 88.

All the electrical components in the present application can be connected with an externally adapted power supply through a wire, and an adapted external controller should be selected to connect according to a specific actual use condition to meet a control requirement for all the electrical components, and a specific connection manner and a control sequence thereof should be referred to in the following working principle, in which the electrical components are electrically connected in sequence, a detailed connection means thereof is a known technology in the art and is not described, and a working principle and a working process thereof are mainly described below).

Step 1: the culture dish containing cells and liquid culture medium is placed on the upper surface of the rotating plate 82 and located on the inner sides of the left and right fixed seats 87 by a worker, the electric push rod 88 and the constant temperature device 4 are sequentially started by the controller 2 while the sealing door 3 is closed, the electric push rod 88 extends to push the arc-shaped clamping plates 89 on the corresponding positions to move inwards, the outer walls of the culture dish are clamped by the left and right arc-shaped clamping plates 89 to fix the culture dish, and the temperature of the inner cavity of the sterile box body 1 is adjusted by the constant temperature device 4 to enable the cultured cells to be at a constant temperature for growth;

step 2: when cells need to be subjected to horizontal direction oscillation culture, the controller 2 controls the first motor 64 to be started, the first motor 64 drives the gear 65 to rotate clockwise, the inner gear ring 63 is meshed with the gear 65, the inner gear ring 63 is enabled to drive the circular plate 62 to rotate under the action of the rotating force of the gear 65, the circular plate 62 is enabled to drive the culture dish to rotate circumferentially with the axis of the circular plate 62 as the center of a circle under the matching of the limiting groove 66, the lead screw nut 611, the lead screw nut 612, the oscillation assembly 7 and the oscillation assembly 8, and the distance between the axes of the circular plates 62 of the circular plate 612 and the radius is set as the distance, so that a culture medium in the culture dish is in full contact with oxygen, and the supply amount of dissolved oxygen;

and step 3: the operator controls the controller 2 to drive the second motor 69, so that the second motor 69 drives the second gear 610 to rotate clockwise or counterclockwise, the first gear 68 is meshed with the second gear 610, the first gear 68 drives the lead screw 67 to rotate counterclockwise or clockwise under the action of the rotation force of the second gear 610, and the lead screw nut 611 is screwed with the lead screw 67, so that the lead screw nut 611 drives the support rod 612 to move inwards or outwards under the action of the rotation force of the lead screw 67 and the limiting action of the limiting groove 66, so as to change the distance between the support rod 612 and the axis of the circular plate 62, and further change the horizontal oscillation amplitude of the cell culture dish;

and 4, step 4: when the cells need to be cultured by shaking in the vertical direction, the operator controls the third motor 72 to drive the cam 73 to rotate clockwise, so that the distal hub end and the proximal hub end of the outer wall of the cam 73 intermittently contact with the lower surface of the rotating assembly housing 81, because the diameters of the distal hub end and the proximal hub end of the cam 73 are different, when the distal hub end of the cam 73 contacts with the lower surface of the rotating assembly housing 81, the rotating assembly housing 81 moves upward under the pushing of the distal hub end of the cam 73, and drives the connecting rod 742 to move upward in the inner cavity of the limiting rod 741 at the corresponding position, so as to achieve the limiting effect, and simultaneously, the connecting rod 742 stretches the limiting spring 743 at the corresponding position, when the proximal hub end of the cam 73 contacts with the lower surface of the rotating assembly housing 81, the limiting spring 743 pulls the rotating assembly housing 81 downward through the connecting rod 742 under the self-elastic effect, so as to promote the rotating assembly, the culture dish fixed in the rotating component 8 is driven to oscillate up and down, and different oscillation modes can be adopted according to requirements so as to improve the sufficient contact area of the culture medium in the culture dish and oxygen;

and 5: when cells need to be subjected to shake culture, a worker controls the rotating assembly 4 to start the fourth motor 85, the fourth motor 85 drives the worm 86 to rotate clockwise, the worm wheel 84 is meshed with the worm 86, the worm wheel 84 drives the rotating shaft 83 to rotate under the rotating force of the worm 86, the rotating shaft 83 drives the culture dish to rotate under the matching of the rotating plate 82, the rotating plate 87, the electric push rod 88 and the arc-shaped clamping plate 89, and then the cells in the culture dish are subjected to rotary culture;

thereby when carrying out the shake culture to the culture dish, can adjust vibration amplitude and oscillation direction as required in time, the suitability is strong to can realize the integration of shake culture and rotatory cultivation, reduce staff's operation item, improve the device practicality.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell shaking incubator, comprising:

a sterile box (1);

the controller (2) is embedded in the right end of the front side of the sterile box body (1);

the sealing door (3) is hinged at the opening at the front side of the inner cavity of the sterile box body (1);

the inner cavity of the constant temperature device (4) is arranged on the left side of the sterile box body (1), the right side of the constant temperature device (4) extends into the inner cavity of the sterile box body (1), and the constant temperature device (4) is electrically connected with the controller (2);

the culture mechanism (5) is arranged at the bottom end of the inner cavity of the sterile box body (1).

2. A cell shaking incubator according to claim 1, wherein: the culture mechanism (5) comprises:

the shaking assembly (6) is embedded in the center of the top end of the inner cavity of the sterile box body (1);

an oscillating assembly (7) arranged at the top end of the shaking assembly (6);

and the rotating assembly (8) is mounted at the top end of the oscillating assembly (7).

3. A cell shaking incubator according to claim 2, wherein: the wobble assembly (6) comprises:

the shaking assembly shell (61) is embedded in the center of the top end of the inner cavity of the sterile box body (1);

the circular plate (62) is rotatably connected to the upper surface of the first motor (64) through a bearing, an inner ring of the bearing is in interference fit with an outer wall of the circular plate (62), an outer ring of the bearing is fixedly connected with an inner wall of the shaking assembly shell (61), and the bottom end of the circular plate (62) extends into an inner cavity of the shaking assembly shell (61);

an inner gear ring (63) provided on a lower surface of the circular plate (62);

the first motor (64) is arranged at the bottom end of the inner cavity of the shaking component shell (61) in the vertical direction, and the first motor (64) is electrically connected with the controller (2);

and the gear (65) is connected to the output end of the first motor (64) through a screw, and the gear (65) is meshed with the inner gear ring (63).

4. A cell shaking incubator according to claim 3, wherein: the wobble assembly (6) further comprises:

a limit groove (66) which is arranged on the upper surface of the circular plate (62) along the left-right direction;

the screw rod screw (67) is rotatably connected to the inner cavity of the limiting groove (66) through a bearing along the left-right direction, an inner ring of the bearing is in interference fit with the outer wall of the screw rod screw (67), and an outer ring of the bearing is fixedly connected with the inner wall of the limiting groove (66);

a first gear (68) which is connected to the left side of the outer wall of the screw rod screw (67) in a key mode;

the second motor (69) is arranged in the middle of the top end of the circular plate (62) along the left direction, and the second motor (69) is electrically connected with the controller (2);

a second gear (610) which is connected with the output end of the second motor (69) in a screw mode, wherein the second gear (610) is meshed with the first gear (68);

the screw rod nut (611) is screwed on the outer wall of the screw rod (67), and the outer wall of the screw rod nut (611) is in adaptive insertion connection with the inner cavity of the limiting groove (66);

and a support rod (612) which is arranged at the top end of the screw nut (611) along the vertical direction.

5. A cell shaking incubator according to claim 2, wherein: the oscillating assembly (7) comprises:

a bottom plate (71) disposed at the top end of the support rod (612);

the third motor (72) is installed at the center of the top end of the bottom plate (71) through a support, and the third motor (72) is electrically connected with the controller (2);

a cam (73) which is connected with the output end of the third motor (72) through a screw;

the number of the limiting units (74) is four, and the four limiting units (74) are respectively arranged at four corners of the top end of the bottom plate (71) along the vertical direction.

6. A cell shaking incubator according to claim 5, wherein: the stopper unit (74) includes:

the number of the limiting rods (741) is four, the four limiting rods (741) are respectively arranged at four corners of the top end of the bottom plate (71) along the vertical direction, and the top end of an inner cavity of each limiting rod (741) is communicated with the outer wall of the corresponding limiting rod;

the number of the connecting rods (742) is four, and the four connecting rods (742) are respectively inserted into the top end of the inner cavity of the limiting rod (741) in the vertical direction;

the limiting device comprises limiting springs (743), the number of the limiting springs (743) is four, the four limiting springs (743) are arranged at the bottom end of an inner cavity of the limiting rod (741) along the up-down direction respectively, and the upper end and the lower end of each limiting spring (743) are fixedly connected with the inner wall of the limiting rod (741) and the bottom end of the connecting rod (742) respectively.

7. A cell shaking incubator according to claim 2, wherein: the rotating assembly (8) comprises:

a rotating assembly housing (81) provided at the top end of the connecting rod (742) in the left-right direction, the lower surface of the rotating assembly housing (81) being in contact with the outer wall of the cam (73);

the rotating plate (82) is rotatably connected to the upper surface of the rotating assembly shell (81) through a bearing, an inner ring of the bearing is in interference fit with the outer wall of the rotating plate (82), an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly shell (81), and the bottom end of the rotating plate (82) extends into an inner cavity of the rotating assembly shell (81);

the rotating shaft (83) is rotatably connected to the bottom end of the inner cavity of the rotating assembly shell (81) through a bearing in the vertical direction, an inner ring of the bearing is in interference fit with the outer wall of the rotating shaft (83), an outer ring of the bearing is fixedly connected with the outer wall of the rotating assembly shell (81), and the top end of the rotating shaft (83) is fixedly connected with the lower surface of the rotating plate (82);

the worm wheel (84) is in interference fit with the top end of the outer wall of the rotating shaft (83);

the fourth motor (85) is arranged at the bottom end of the inner cavity of the rotating assembly shell (81) in the left-right direction, and the fourth motor (85) is electrically connected with the controller (2);

and the worm (86) is in screw connection with the output end of the fourth motor (85) along the left-right direction, and the worm (86) is meshed with the worm wheel (84).

8. A cell shaking incubator according to claim 7, wherein: the rotating assembly (8) further comprises:

the number of the fixed seats (87) is two, and the two fixed seats (87) are respectively arranged on the left side and the right side of the top end of the rotating plate (82);

the number of the electric push rods (88) is two, the two electric push rods (88) are respectively arranged on the outer sides of the left fixing seat and the right fixing seat (87), the inner sides of the electric push rods (88) extend into the inner sides of the fixing seats (87), and the electric push rods (88) are electrically connected with the controller (2);

arc pinch-off blades (89), the quantity of arc pinch-off blades (89) is two, two arc pinch-off blades (89) set up the inboard of controlling two electric push rods (88) respectively.