CN112342122A - Anti-pollution type bacterial culture device and bacterial culture method for clinical laboratory - Google Patents
Anti-pollution type bacterial culture device and bacterial culture method for clinical laboratory Download PDFInfo
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Abstract
The invention relates to the technical field of bacterial culture, and discloses an anti-pollution bacterial culture device for a clinical laboratory and a bacterial culture method. The culture dish is reasonable in design, the culture solution is stirred by the cooperation of the culture dish and the stirring rod while the culture dish is driven to rotate, so that the culture solution is circulated, thalli are suspended, the illumination effect is ensured, oxygen can be introduced into the culture dish to supplement oxygen required by growth of aerobic bacteria, and the thallus culture effect is ensured.
Description
Technical Field
The invention relates to the technical field of bacterial culture, in particular to an anti-pollution bacterial culture device and a bacterial culture method for a clinical laboratory.
Background
Bacterial culture is a technique in which bacteria are grown and propagated artificially. The bacteria are widely distributed in nature, large in quantity and multiple in types, can benefit mankind, and can also become a cause of diseases. Most bacteria can be cultured artificially by inoculating them on a culture medium and allowing them to grow and propagate. The cultured bacteria are used for research, identification and application. Aeration or stirring is required in the process of culturing the photosynthetic bacteria, and the function is to help the precipitated photosynthetic bacteria float upwards to obtain illumination and keep the good growth of bacterial cells. In small-scale anaerobic culture, bacterial cells are floated by shaking a culture container manually or the bacterial cells are kept suspended by using a small water pump to make culture solution slowly circulate.
Prior patent CN104531518B discloses a contamination-proof bacteria culture device for clinical laboratory, which can regulate and control the temperature and illumination in the device. However, at least the following technical problems still exist in the prior art device: 1) the device can't rock the culture dish, the motion is single, and the automaticity is poor, lead to bacterium come-up at the bottom of the efficiency, influence culture effect, 2) the device can't adjust the oxygen content in the device, can't be applicable to the cultivation of the bacterium that the oxygen demand is different simultaneously, 3) the device can't aerify, just can't make culture solution circulation operation to the culture dish, and the function is single, therefore we have proposed an anti-pollution formula bacteria culture device of clinical laboratory and bacteria culture method and are used for solving above-mentioned problem.
Disclosure of Invention
The invention aims to solve the defects that the prior bacteria culture device generally stirs and shakes a culture dish manually, has single movement mode and poor automation, causes low bacteria floating efficiency, influences the culture effect, needs to inflate the culture dish by an air pump and enables a culture solution to circularly operate by a water pump, causes complex device structure and large energy consumption, and provides an anti-pollution bacteria culture device and a bacteria culture method for a clinical laboratory.
In order to achieve the purpose, the invention adopts the following technical scheme:
an anti-pollution type bacteria culture device for a clinical laboratory comprises a box body, a culture dish and a placing table, wherein a vertical plate is fixedly arranged on the inner wall of one side of the box body through bolts, a transverse plate is fixedly arranged on one side of the vertical plate, an empty box is fixedly arranged on one side of the transverse plate, the bottom of the empty box is communicated with one end of a straight pipe, the other end of the straight pipe extends into the culture dish, a plurality of stirring rods are fixedly arranged on the outer side of the straight pipe, a placing groove is formed in the top of the placing table, the culture dish is placed in the placing groove, a supporting plate is rotatably connected to the bottom of the placing table through a hinge, a sleeve plate is slidably sleeved on the outer side of the supporting plate, one side of the top of the sleeve plate is rotatably connected with one end of a connecting rod through a hinge, the other end of the connecting rod is, a motor is fixedly arranged on the inner wall of the other side of the box body, one end of a worm is fixedly connected to an output shaft of the motor, one end of a transverse shaft is fixedly connected to the other end of the worm, bevel gears are fixedly arranged on the other end of the transverse shaft and a vertical shaft, the two bevel gears are meshed with each other, one end of two vertical rods is fixedly connected to the inner wall of the bottom of the box body, the outer sides of the two vertical rods are slidably sleeved with a sliding plate, one end of a push rod is fixedly connected to one side of the top of the sliding plate and is abutted against the bottom of a sleeve plate, a cam is fixedly arranged on the transverse shaft and is in rolling connection with the bottom of the sliding plate, a mounting plate is fixedly arranged on the inner wall of the other side of the box body, a barrel is fixedly arranged on the top, and fixed installation control valve on the trachea, sliding seal installs the piston plate in the barrel, and piston plate bottom fixed mounting has the push pedal, and the barrel slides and cup joints in the push pedal outside, fixed mounting has the fixed plate on the inner wall of box one side, and the fixed plate front side rotates and installs the worm wheel, and equal fixed mounting has the circle axle bottom worm wheel front side and push pedal front side, and two circle axle outsides rotate and cup joint same connecting plate.
Preferably, a limiting plate is fixedly mounted at the front end of the circular shaft, and the limiting plate is movably abutted against the front side of the connecting plate to limit the connecting plate.
Preferably, the two sides of the supporting plate are fixedly provided with the baffle, the bottom of the baffle is fixedly connected with one end of a connecting spring, and the other end of the connecting spring is fixedly connected with the top of the sleeve plate to reset the sleeve plate.
Preferably, place platform one side and seted up threaded hole, the threaded hole internal thread is installed the threaded rod, and threaded rod one end is convenient for fix the culture dish with culture dish outside activity butt.
Preferably, a positioning plate is fixedly mounted on the inner wall of the bottom of the box body, and the positioning plate is rotatably sleeved on the outer side of the cross shaft to rotatably position the cross shaft.
Preferably, two montant top fixed mounting have same roof, and roof bottom fixedly connected with two reset spring's one end, the top of the equal fixedly connected with slide of the other end of two reset spring resets the slide.
Preferably, the box top is the opening setting to be connected with the case lid through the hinge rotation, case lid and box top activity sealing butt, box one side intercommunication has the one end of breather pipe, is convenient for open and seal the box.
The invention also provides a method for culturing pollution-proof bacteria in the clinical laboratory, which comprises the following steps:
s1: opening the box cover, screwing the direction bolt to disassemble the vertical plate, then placing the culture dish in the placing groove, driving the threaded rod to move towards one side close to the culture dish by rotating the threaded rod, and fixing the culture dish in the placing groove by abutting the threaded rod with the outer side of the culture dish;
s2: the vertical plate is installed back to the original position through the bolt, then the box cover is closed, and air in the box body is replaced through the ventilation pipe;
s3: the motor is started, the motor output shaft drives the worm to rotate, the worm drives the transverse shaft to rotate, the transverse shaft drives the vertical shaft to rotate through the meshing between the two bevel gears, the vertical shaft drives the supporting plate to rotate, the supporting plate drives the placing table to rotate, meanwhile, the transverse shaft drives the cam to rotate, the cam pushes the sliding plate to move upwards and compress the return spring, the sliding plate drives the push rod to move upwards, the push rod pushes the sleeve plate to move upwards and compress the connecting spring, the sleeve plate drives one side of the placing table to move upwards through the connecting rod, the cam continues to rotate, the sliding plate returns to move downwards under the elastic force of the return spring, the sleeve plate returns to slide reversely under the elastic force of the connecting spring and drives one side of the placing table to move downwards through the connecting rod, the cam continues to rotate and drives the sleeve plate to reciprocate upwards and downwards, so that the placing table, the culture solution in the culture dish is stirred through the cooperation of the culture dish and a fixedly arranged stirring rod, so that the thallus floats upwards, and the precipitated photosynthetic bacteria float upwards to obtain illumination, and the good growth of the photosynthetic bacteria is ensured;
s4: the worm drives the worm wheel to rotate in the rotating process, the worm wheel drives the circular shaft positioned below to do circular motion based on the rotating center of the worm wheel and drives the push plate to do up-and-down reciprocating motion through the connecting plate, the push plate drives the piston plate to slide up and down in the cylinder body in a reciprocating manner, when aerobic bacteria are cultured, the control valve is opened, the air pressure in the empty box is reduced in the descending process of the piston plate, the culture solution enters the empty box through the straight pipe, when the piston plate descends to the position below the air pipe, the air in the air pipe enters the cylinder body, the culture solution in the empty box flows back to the culture dish under the action of gravity, the oxygen in the cylinder body is pushed to enter the culture dish through the communicating pipe, the empty box and the straight pipe in the ascending process of the piston plate to supplement the oxygen required by the growth of the aerobic bacteria, meanwhile, the shaking of the culture dish and the stirring, thereby prevent that oxygen from passing through in the trachea gets into the culture dish, the up-and-down reciprocating motion of piston plate drives the empty case and carries out intermittent type nature absorption and release to the culture solution in the culture dish to make the culture solution form the circulation, make the thallus suspension.
Compared with the prior art, the invention provides an anti-pollution type bacterial culture device and a bacterial culture method for a clinical laboratory, and the anti-pollution type bacterial culture device and the bacterial culture method have the following beneficial effects:
(1) the placing table and the culture dish are driven by the cooperation of the components to rotate around the vertical shaft and shake up and down, and culture solution in the culture dish is stirred by the cooperation of the placing table and the culture dish and a fixedly arranged stirring rod, so that thalli float upwards, and precipitated photosynthetic bacteria float upwards to obtain illumination and ensure good growth of the photosynthetic bacteria;
(2) the invention can be simultaneously suitable for the culture of bacteria with different oxygen demands, when aerobic bacteria are cultured, oxygen in the trachea is introduced into the culture dish, oxygen required by the growth of the aerobic bacteria is supplemented, meanwhile, culture solution is circulated and operated, and meanwhile, the shaking of the culture dish and the stirring of the stirring rod can ensure that the oxygen is distributed more uniformly;
(3) when cultivateing anaerobic bacteria, the control valve prevents that oxygen from passing through in the trachea gets into the culture dish, and the up-and-down reciprocating motion of piston plate drives the empty case and carries out intermittent type nature absorption and release to the culture solution in to the culture solution formation circulation makes the thallus suspension, guarantees the illumination effect, thereby guarantees the effect that the thallus was cultivateed.
The invention can realize stirring of the culture solution on the whole, can realize up-and-down shaking to suspend the thalli, can adjust the oxygen content in the equipment, is suitable for culturing different types of bacteria, and in addition, by reasonably arranging the components, the mechanical motion of the invention can be realized by only using one power source, thereby saving energy.
Drawings
FIG. 1 is a schematic cross-sectional structural view of an anti-pollution type bacteria culture device in a clinical laboratory according to the present invention;
FIG. 2 is a front view of an antipollution bacteria culturing device for clinical laboratory according to the present invention;
FIG. 3 is a schematic structural view of part A of an anti-pollution type bacteria culture device in a clinical laboratory according to the present invention;
FIG. 4 is a schematic structural view of part B of an anti-pollution type bacteria culture device in a clinical laboratory according to the present invention;
fig. 5 is a right side view of the race plate, cross shaft, slide plate, cam and push rod of the present invention.
In the figure: 1. a box body; 2. a culture dish; 3. a box cover; 4. a placing table; 5. a placement groove; 6. a support plate; 7. sheathing; 8. a connecting rod; 9. a motor; 10. a worm; 11. a horizontal axis; 12. a vertical axis; 13. a bevel gear; 14. a vertical rod; 15. a slide plate; 16. a cam; 17. a push rod; 18. a vertical plate; 19. a transverse plate; 20. empty boxes; 21. a barrel; 22. a communicating pipe; 23. an air tube; 24. a control valve; 25. a fixing plate; 26. a worm gear; 27. a circular shaft; 28. pushing the plate; 29. a connecting plate; 30. a limiting plate; 31. a piston plate; 32. a straight pipe; 33. a stirring rod; 34. a threaded rod; 35. a baffle plate; 36. a connecting spring; 37. a top plate; 38. a return spring; 39. and (7) positioning the plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-5, an anti-pollution type bacteria culture device for clinical laboratory, comprising a box body 1, a culture dish 2 and a placing table 4, wherein a vertical plate 18 is fixedly arranged on the inner wall of one side of the box body 1 through bolts, a horizontal plate 19 is fixedly arranged on one side of the vertical plate 18, an empty box 20 is fixedly arranged on one side of the horizontal plate 19, the bottom of the empty box 20 is communicated with one end of a straight pipe 32, the other end of the straight pipe 32 extends into the culture dish 2, a plurality of stirring rods 33 are fixedly arranged on the outer side of the straight pipe 32, a placing groove 5 is arranged on the top of the placing table 4, the culture dish 2 is placed in the placing groove 5, the bottom of the placing table 4 is rotatably connected with a supporting plate 6 through hinges, a sleeve plate 7 is slidably sleeved on the outer side of the supporting plate 6, one side of the top of the sleeve plate 7 is rotatably connected with one end of, the inner wall of the bottom of the box body 1 is provided with a mounting groove, a vertical shaft 12 is rotatably arranged in the mounting groove, the inner wall of the other side of the box body 1 is fixedly provided with a motor 9, the output shaft of the motor 9 is fixedly connected with one end of a worm 10, the other end of the worm 10 is fixedly connected with one end of a horizontal shaft 11, the other end of the horizontal shaft 11 and the vertical shaft 12 are both fixedly provided with bevel gears 13, the two bevel gears 13 are meshed with each other, the inner wall of the bottom of the box body 1 is fixedly connected with one end of two vertical rods 14, the outer sides of the two vertical rods 14 are slidably sleeved with a same sliding plate 15, one side of the top of the sliding plate 15 is fixedly connected with one end of a push rod 17, the push rod 17 is abutted against the bottom of a sleeve plate 7, the horizontal shaft 11 is fixedly provided with a cam 16, the cam 16 is in rolling connection with the bottom of, thereby pushing the slide plate 15 to move up and down. Fixed mounting has the mounting panel on the 1 opposite side inner wall of box, mounting panel top fixed mounting has barrel 21, barrel 21 top intercommunication has the one end of communicating pipe 22, the other end and the top of empty case 20 one side of communicating pipe 22 are linked together, barrel 21 one side intercommunication has the one end of trachea 23, the other end of trachea 23 extends to the 1 outside of box, and fixed mounting control valve 24 on the trachea 23, sliding seal installs piston plate 31 in barrel 21, piston plate 31 bottom fixed mounting has push pedal 28, barrel 21 slides and cup joints in the push pedal 28 outside, fixed mounting has fixed plate 25 on the 1 one side inner wall of box, fixed plate 25 front side rotates and installs worm wheel 26, equal fixed mounting has round axle 27 bottom worm wheel 26 front side and push pedal 28 front side, two round axle 27 outsides rotate and cup joint same connecting plate 29.
In this embodiment, circular shaft 27 front end fixed mounting has limiting plate 30, and limiting plate 30 and the movable butt of connecting plate 29 front side carry on spacingly to connecting plate 29, and 6 both sides of backup pad fixed mounting have baffle 35, and baffle 35 bottom fixedly connected with connecting spring 36's one end, connecting spring 36's the other end and lagging 7 top fixed connection reset lagging 7.
In this embodiment, place 4 one sides of platform and seted up threaded hole, threaded hole internal thread installs threaded rod 34, and threaded rod 34 one end is convenient for fix culture dish 2 with 2 outside activity butts in the culture dish, rotates the location to cross axle 11.
In this embodiment, two 14 top fixed mounting of montants have same roof 37, roof 37 bottom two reset spring 38's of fixedly connected with one end, the top of the equal fixedly connected with slide 15 of the other end of two reset spring 38, reset slide 15, 1 top of box is the opening setting, and be connected with case lid 3 through hinge rotation, case lid 3 and the sealed butt of 1 top activity of box, 1 one side intercommunication of box has the one end of taking a breath the pipe, be convenient for open and seal box 1.
The embodiment also provides a method for culturing pollution-prevention bacteria in the clinical laboratory, which comprises the following steps:
s1: opening the box cover 3, screwing the bolts to detach the vertical plates 18, then placing the culture dish 2 in the placing groove 5, driving the threaded rod 34 to move towards one side close to the culture dish 2 by rotating the threaded rod 34, and fixing the culture dish 2 in the placing groove 5 by abutting the threaded rod 34 with the outer side of the culture dish 2;
through the cooperation of bolt and riser 18, the convenience is dismantled the culture dish, is convenient for change the washing, through the cooperation of threaded rod 34 with culture dish 2, can be applicable to the fixed to the culture dish of different bottom surface diameters, and the suitability is wider.
S2: the vertical plate 18 is installed back to the original position through a bolt, then the box cover 3 is closed, and the air in the box body 1 is replaced through a ventilation pipe;
s3: starting the motor 9, the output shaft of the motor 9 drives the worm 10 to rotate, the worm 10 drives the transverse shaft 11 to rotate, the transverse shaft 11 drives the vertical shaft 12 to rotate through the meshing between the two bevel gears 13, the vertical shaft 12 drives the supporting plate 6 to rotate, the supporting plate 6 drives the placing table 4 to rotate, meanwhile, the transverse shaft 11 drives the cam 16 to rotate, the cam 16 pushes the sliding plate 15 to move upwards and compress the return spring 38, the sliding plate 15 drives the push rod 17 to move upwards, the push rod 17 pushes the sleeve plate 7 to move upwards and compress the connecting spring 36, the sleeve plate 7 drives one side of the placing table 4 to move upwards through the connecting rod 8, the cam 16 continues to rotate, the sliding plate 15 returns to move downwards under the elastic force of the return spring 38, the sleeve plate 7 returns to slide reversely under the elastic force of the connecting spring 36 and drives one side of the placing table 4 to move downwards through the connecting rod 8, and the cam 16 continuously, thereby driving the placing platform 4 and the culture dish 2 to rotate around the vertical shaft 12 as an axis and simultaneously shake up and down, and stirring the culture solution in the culture dish 2 through the cooperation with the fixedly arranged stirring rod 33 to enable the thalli to float up, so that the precipitated photosynthetic bacteria float up to obtain illumination and ensure good growth of the photosynthetic bacteria;
s4: the worm 10 drives the worm wheel 26 to rotate in the rotating process, the worm wheel 26 drives the circular shaft 27 located below to do circular motion based on the rotating center of the worm wheel 26, the push plate 28 is driven to do reciprocating motion up and down by the connecting plate 29, the push plate 28 drives the piston plate 31 to do reciprocating motion up and down in the cylinder 21, when aerobic bacteria are cultured, the control valve 24 is opened, the pressure in the empty box 20 is reduced in the descending process of the piston plate 31, the culture solution enters the empty box 20 through the straight pipe 32, when the piston plate 31 descends to the lower part of the air pipe 23, the air in the air pipe 23 enters the cylinder 21, the culture solution in the empty box 20 returns to the culture dish 2 under the action of gravity, the oxygen in the cylinder 21 is pushed by the ascending process of the piston plate 31 to enter the culture dish 2 through the communicating pipe 22, the empty box 20 and the straight pipe 32, the culture solution can circulate, the oxygen required by the aerobic bacteria can be supplemented, meanwhile, the shaking of the culture dish 2 and the stirring of the, when cultivateing anaerobic bacteria, closing control valve 24 to prevent that oxygen from passing through trachea 23 entering culture dish 2 in, the up-and-down reciprocating motion of piston plate 31 drives empty case 20 and carries out intermittent type nature's absorption and release to the culture solution in the culture dish 2, thereby makes the culture solution form the circulation, makes the thallus suspension, guarantees the illumination effect, thereby guarantees the effect that the thallus was cultivateed.
The standard parts used in the invention can be purchased from the market, the special-shaped parts can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts conventional means such as bolts, rivets, welding and the like mature in the prior art, the machines, the parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, so that the detailed description is omitted.
Claims (8)
1. An anti-pollution type bacteria culture device for a clinical laboratory comprises a box body (1), a culture dish (2) and a placing table (4), and is characterized in that a vertical plate (18) is fixedly arranged on the inner wall of one side of the box body (1) through bolts, a transverse plate (19) is fixedly arranged on one side of the vertical plate (18), an empty box (20) is fixedly arranged on one side of the transverse plate (19), the bottom of the empty box (20) is communicated with one end of a straight pipe (32), the other end of the straight pipe (32) extends into the culture dish (2), a plurality of stirring rods (33) are fixedly arranged on the outer side of the straight pipe (32), a placing groove (5) is formed in the top of the placing table (4), the culture dish (2) is placed in the placing groove (5), a supporting plate (6) is rotatably connected to the bottom of the placing table (4) through hinges, a sleeve plate (7) is slidably sleeved on the outer side of the supporting plate (6), one side of, the other end of the connecting rod (8) is rotatably connected with one side of the bottom of the placing table (4) through a hinge, one end of a vertical shaft (12) is fixedly connected with the bottom of the supporting plate (6), a mounting groove is formed in the inner wall of the bottom of the box body (1), the vertical shaft (12) is rotatably mounted in the mounting groove, a motor (9) is fixedly mounted on the inner wall of the other side of the box body (1), one end of a worm (10) is fixedly connected onto an output shaft of the motor (9), the other end of the worm (10) is fixedly connected with one end of a transverse shaft (11), bevel gears (13) are fixedly mounted on the other end of the transverse shaft (11) and the vertical shaft (12), the two bevel gears (13) are meshed with each other, one end of two vertical rods (14) is fixedly connected onto the inner wall of the bottom of the box body (1), the same sliding plate (15) is, push rod (17) and lagging (7) bottom butt, fixed mounting has cam (16) on cross axle (11), cam (16) and slide (15) bottom roll connection, fixed mounting has the mounting panel on box (1) opposite side inner wall, and mounting panel top fixed mounting has barrel (21), and barrel (21) top intercommunication has the one end of communicating pipe (22), and the other end and the top of empty case (20) one side of communicating pipe (22) are linked together, barrel (21) one side intercommunication has the one end of trachea (23), and the other end of trachea (23) extends to the box (1) outside, and fixed mounting control valve (24) on trachea (23), sliding seal installs piston plate (31) in barrel (21), and piston plate (31) bottom fixed mounting has push pedal (28), and barrel (21) slip cup joints in the push pedal (28) outside, fixed mounting has fixed plate (25) on box (1) one side inner wall, the front side of the fixed plate (25) is rotatably provided with a worm wheel (26), the front side of the worm wheel (26) and the bottom of the front side of the push plate (28) are fixedly provided with round shafts (27), and the outer sides of the two round shafts (27) are rotatably sleeved with the same connecting plate (29).
2. The contamination-proof type bacteria culture device for the clinical laboratory according to claim 1, wherein a limiting plate (30) is fixedly mounted at the front end of the circular shaft (27), and the limiting plate (30) is movably abutted against the front side of the connecting plate (29).
3. The contamination-proof type bacteria culture device for the clinical laboratory according to claim 1, wherein a baffle plate (35) is fixedly mounted on each of two sides of the support plate (6), one end of a connecting spring (36) is fixedly connected to the bottom of the baffle plate (35), and the other end of the connecting spring (36) is fixedly connected to the top of the sleeve plate (7).
4. The contamination-proof type bacteria culture device for the clinical laboratory according to claim 1, wherein a threaded hole is formed in one side of the placing table (4), a threaded rod (34) is mounted in the threaded hole in a threaded manner, and one end of the threaded rod (34) is movably abutted against the outer side of the culture dish (2).
5. The contamination-proof bacteria culture device for the clinical laboratory according to claim 1, wherein a positioning plate (39) is fixedly mounted on the inner wall of the bottom of the box body (1), and the positioning plate (39) is rotatably sleeved on the outer side of the transverse shaft (11).
6. The contamination-proof bacteria cultivation device for clinical laboratory according to claim 1, characterized in that the top end of two vertical rods (14) is fixedly installed with the same top plate (37), one end of two return springs (38) is fixedly connected to the bottom of the top plate (37), and the other end of two return springs (38) is fixedly connected to the top of the sliding plate (15).
7. The contamination-proof type bacteria culture device for the clinical laboratory according to claim 1, wherein the top of the box body (1) is provided with an opening, a box cover (3) is rotatably connected through a hinge, the box cover (3) is movably and hermetically abutted against the top of the box body (1), and one end of a ventilation pipe is communicated with one side of the box body (1).
8. An anti-pollution type bacterial culture method for a clinical laboratory is characterized by comprising the following steps:
s1: the box cover (3) is opened, the vertical plate (18) is disassembled by screwing the bolt, then the culture dish (2) is placed in the placing groove (5), the threaded rod (34) is driven to move towards one side close to the culture dish (2) by rotating the threaded rod (34), and the culture dish (2) is fixed in the placing groove (5) by the abutting joint of the threaded rod (34) and the outer side of the culture dish (2);
s2: the vertical plate (18) is installed back to the original position through a bolt, then the box cover (3) is closed, and air in the box body (1) is replaced through a ventilation pipe;
s3: starting a motor (9), driving a worm (10) to rotate by an output shaft of the motor (9), driving a transverse shaft (11) to rotate by the worm (10), driving a vertical shaft (12) to rotate by the transverse shaft (11) through meshing between two bevel gears (13), driving a supporting plate (6) to rotate by the vertical shaft (12), driving a placing table (4) to rotate by the supporting plate (6), driving a cam (16) to rotate by the transverse shaft (11), driving a sliding plate (15) to move upwards and compressing a return spring (38) by the cam (16), driving a push rod (17) to move upwards by the sliding plate (15), driving a sleeve plate (7) to move upwards and compressing a connecting spring (36), driving one side of the placing table (4) to move upwards by the connecting rod (8) by the sleeve plate (7), continuously rotating the cam (16), and resetting the sliding plate (15) to move downwards under the elastic force of the return spring (38), the sleeve plate (7) resets and slides reversely under the elastic force action of the connecting spring (36), the connecting rod (8) drives one side of the placing table (4) to move downwards, the cam (16) continuously rotates to drive the sleeve plate (7) to reciprocate up and down, so that the placing table (4) and the culture dish (2) are driven to swing up and down while rotating by taking the vertical shaft (12) as an axis, culture solution in the culture dish (2) is stirred through the cooperation of the cam and a fixedly arranged stirring rod (33), thalli are floated upwards, and precipitated photosynthetic bacteria are floated upwards to obtain illumination, and good growth of the photosynthetic bacteria is guaranteed;
s4: the worm (10) drives a worm wheel (26) to rotate in the rotating process, the worm wheel (26) drives a circular shaft (27) located below to do circular motion based on the rotating center of the worm wheel (26), and drives a push plate (28) to do up-and-down reciprocating motion through a connecting plate (29), the push plate (28) drives a piston plate (31) to do up-and-down reciprocating sliding in a cylinder body (21), when aerobic bacteria are cultured, a control valve (24) is opened, in the descending process of the piston plate (31), the air pressure in an empty box (20) is reduced, a culture solution enters the empty box (20) through a straight pipe (32), when the piston plate (31) descends to the lower side of an air pipe (23), the air in the air pipe (23) enters the cylinder body (21), the culture solution in the empty box (20) flows back to a culture dish (2) under the action of gravity, and in the ascending process of the piston plate (31), the oxygen in the cylinder body (21) is pushed to pass, Empty case (20) and straight tube (32) get into in culture dish (2), supply the required oxygen of aerobic bacteria growth, the rocking of culture dish (2) can make oxygen distribution more even with the stirring of puddler (33) simultaneously, when cultivateing anaerobic bacteria, close control valve (24), thereby prevent that oxygen from passing through in trachea (23) gets into culture dish (2), the up-and-down reciprocating motion of piston plate (31) drives empty case (20) and carries out intermittent type nature's absorption and release to the culture solution in culture dish (2), thereby make the culture solution form the circulation, make the thallus suspension.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011251272.0A CN112342122A (en) | 2020-11-11 | 2020-11-11 | Anti-pollution type bacterial culture device and bacterial culture method for clinical laboratory |
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| CN202011251272.0A CN112342122A (en) | 2020-11-11 | 2020-11-11 | Anti-pollution type bacterial culture device and bacterial culture method for clinical laboratory |
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Application publication date: 20210209 |