CN202465713U - Low-speed stirring system for cell culture - Google Patents
Low-speed stirring system for cell culture Download PDFInfo
- Publication number
- CN202465713U CN202465713U CN 201220096358 CN201220096358U CN202465713U CN 202465713 U CN202465713 U CN 202465713U CN 201220096358 CN201220096358 CN 201220096358 CN 201220096358 U CN201220096358 U CN 201220096358U CN 202465713 U CN202465713 U CN 202465713U
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- CN
- China
- Prior art keywords
- cell
- magnetic
- stirring
- culturing bottle
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a low-speed stirring system for cell culture, and relates to a piece of laboratory equipment, in particular to a stirring device for cell culture. The low-speed stirring system for cell culture comprises a controller, a magnetic rotor for cell culture, a magnetic cell stirring and culturing bottle bracket, and a magnetic cell stirring and culturing bottle, wherein the magnetic cell stirring and culturing bottle bracket is fixed on the magnetic rotor for cell culture, and the magnetic cell stirring and culturing bottle is arranged on the magnetic cell stirring and culturing bottle bracket; a stator coil and a rotor iron core arranged in the centre of the stator coil are arranged in the magnetic rotor for cell culture; a magnetic stirring unit is arranged in the magnetic cell stirring and culturing bottle, and the magnetic stirring unit is connected with the rotor iron core; and the controller is connected with the magnetic rotor for cell culture. The low-speed stirring system for cell culture solves the problem that the method for preparing the needed cell expression product or culturing the relative cells by using the prior art has shortcomings of time and labor consumption, high cost and easiness in pollution, and solves the problem of the differentiation of the cells.
Description
Technical field
The utility model relates to experiment and uses device, is specifically related to the whipping appts that cell cultures is used.
Background technology
Conventional cell is cultivated and is used square vase, or petridish, for the cultivation of suspension cell, then adopts square vase or petridish are placed on the cell cultures vibrator.With since the effective culture volume of each square vase probably also have only about 50ml.Thereby no matter be for the small-scale preparation of various representations of cell or cell or for the research work of cell cultures, generally need tens or even tens square vases to cultivate, and also can spend great amount of time therebetween.Simultaneously owing to want the numerous square vase of operation amount, cannot say for sure potential pollution problem in the operating process, and the differentiation between cell batch.There is consuming time, consumption power in existing installation if prepare required cell expressing thing or turn out relevant cell, and cost is high, is prone to pollute, and can make cell the shortcoming of differentiation occur simultaneously.
The utility model content
The utility model provides a kind of low speed cell cultures stirring system; The utility model has solved prior art for preparing and has gone out required cell expressing thing or turn out relevant cell, has consuming time, consumption power, and cost is high; Be prone to pollute, can make cell the problem of differentiation occur simultaneously.
For addressing the above problem; The utility model adopts following technical scheme: low speed cell cultures stirring system; Constitute with magnetic force rot 2, cell magnetic agitation culturing bottle support 3 and cell magnetic agitation culturing bottle 4 by unit 1, cell cultures; Cell cultures is provided with cell magnetic agitation culturing bottle 4 with being fixed with a cell magnetic agitation culturing bottle support 3 on the magnetic force rot 2 above the cell magnetic agitation culturing bottle support 3; Cell cultures is with stator coil 2-2 and the rotor core 2-1 that is arranged at stator coil 2-2 center are arranged in the magnetic force rot 2; Be provided with magnetic agitation unit 4-1 in the cell magnetic agitation culturing bottle 4, magnetic agitation unit 4-1 connects rotor core 2-1; Unit 1 connects cell cultures with magnetic force rot 2.
The utility model utilizes the limited condition in laboratory, realizes fast and effeciently preparing required cell expressing thing or turning out relevant cell; Can effectively avoid time-consuming, the effort of traditional method, the big problem of loss.
The utility model is a small-sized low speed cell cultures stirring system, and is economical and practical, can just satisfy a needed cell concentration of experiment level cell jar, is fit to experiment rank and pilot scale primary operation.
The utility model has solved traditional square vase cultivation and has taken time and effort to experiment level cell jar seed culture, and cost is high, is prone to pollute, and can make cell the differentiation problem occur simultaneously.The low speed cell cultures stirring system that the utility model provides can effective intensive culturing cell, saves incubation time, energy and cost, can farthest reduce the differentiation between cell batch simultaneously, reduces Pollution risk.The volume of cell magnetic agitation culturing bottle is about 500ml, approximately is 10 times of T225 square vase, and the cell concentration of 1 culturing bottle just can satisfy an experiment level cell jar inoculation demand.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the mounting structure synoptic diagram of the utility model cell cultures with the magnetic force rot.
Nomenclature among the figure: unit 1, cell cultures are with magnetic force rot 2, stator coil 2-2, rotor core 2-1, cell magnetic agitation culturing bottle support 3, cell magnetic agitation culturing bottle 4, magnetic agitation unit 4-1, supply lead 5.
Embodiment
With illustrated embodiments the utility model is done detailed explanation below.
Shown in Fig. 1-2; Low speed cell cultures stirring system; Constitute with magnetic force rot 2, cell magnetic agitation culturing bottle support 3 and cell magnetic agitation culturing bottle 4 by unit 1, cell cultures; Cell cultures is provided with cell magnetic agitation culturing bottle 4 with being fixed with a cell magnetic agitation culturing bottle support 3 on the magnetic force rot 2 above the cell magnetic agitation culturing bottle support 3; Cell cultures is with stator coil 2-2 and the rotor core 2-1 that is arranged at stator coil 2-2 center are arranged in the magnetic force rot 2; Be provided with magnetic agitation unit 4-1 in the cell magnetic agitation culturing bottle 4, magnetic agitation unit 4-1 connects rotor core 2-1; Unit 1 connects cell cultures with magnetic force rot 2.Culturing bottle 4 keeps it stable through cell magnetic agitation culturing bottle support 3.
Said cell cultures is with magnetic force rot 2, and stator coil 2-2, rotor core 2-1 structure are identical with electric machine structure and principle.
The utility model centers on the intensive culture apparatus of cell before jar cultivation on the cell first, has invented this kind of low speed cell cultures stirring system.This stirring system can effective intensive culturing cell, saves incubation time, energy and cost, can farthest reduce the differentiation between cell batch simultaneously.Generally the maximum surface-area like the commercialization square vase is 225cm
2(T225 square vase), working volume is generally 50ml, and the working volume of a cell blender jar is 500ml.An experiment level cell jar seed dose needs tens square vases to cultivate; If adopt low speed cell cultures stirring system, 1-2 cell blender jar just can satisfy the inoculation demand, therefore can improve the working efficiency of cell cultures greatly; Reduce Pollution risk, reduce the cell differentiation that operation causes.This machine mainly is intended for middle-size and small-size pharmaceutical factory, biological enterprise, and a grade cell jar seed culture operation experimentizes.
The cell cultures stirring system is constantly removed through rotation through the refuse that stirring produces cellular metabolism, and new nutritive ingredient constantly moves to cell peripheral.Ultralow stirring velocity can guarantee that cell rises in the middle of the suspension-s with alap speed, avoids the excessive turbulent flow of media, eliminates the damage of pair cell, improves the yield of cell.Therefore, small-sized square vase cultivation is compared with immobilized, and the cell cultures stirring system not only can increase the working volume of cultivation on a large scale, and through stirring, can guarantee that cell can absorb the growth of nutrient, optimization cell uniformly.This practical cell cultures stirring system, the blender jar that can to place 1 working volume be 500ml is very suitable for breadboard conventional cell cultures, because advantages of small volume can be matched with any incubator rapidly.
The square vase that the utility model cultivate to use with respect to conventional cell, that the cell cultures stirring system has is convenient, safe, advantage fast; With respect to traditional training mode, have and take up room for a short time, experimental situation is on every side required low, be convenient to supporting cell culture incubator, advantage such as economical and practical.
What should explain at last is: obviously, the foregoing description only be for explain clearly that the utility model does for example, and be not qualification to embodiment.For the those of ordinary skill in affiliated field, on the basis of above-mentioned explanation, can also make other multi-form variation or change.Here need not also can't give exhaustive to all embodiments.And conspicuous variation of being amplified out thus or change still are among the protection domain of the utility model.
Claims (1)
1. low speed cell cultures stirring system; It is characterized in that; Constitute with magnetic force rot (2), cell magnetic agitation culturing bottle support (3) and cell magnetic agitation culturing bottle (4) by unit (1), cell cultures; Cell cultures is provided with cell magnetic agitation culturing bottle (4) with being fixed with a cell magnetic agitation culturing bottle support (3) on the magnetic force rot (2) above the cell magnetic agitation culturing bottle support (3); Cell cultures is with stator coil (2-2) being arranged in the magnetic force rot (2) and being arranged at the rotor core (2-1) at stator coil (2-2) center; Be provided with magnetic agitation unit (4-1) in the cell magnetic agitation culturing bottle (4), magnetic agitation unit (4-1) connects rotor core (2-1); Unit (1) connects cell cultures with magnetic force rot (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220096358 CN202465713U (en) | 2012-03-15 | 2012-03-15 | Low-speed stirring system for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220096358 CN202465713U (en) | 2012-03-15 | 2012-03-15 | Low-speed stirring system for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202465713U true CN202465713U (en) | 2012-10-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220096358 Expired - Fee Related CN202465713U (en) | 2012-03-15 | 2012-03-15 | Low-speed stirring system for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202465713U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085855A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
| CN106085856A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of combination type organizational project dynamic cultivation instrument |
| CN106318868A (en) * | 2016-08-22 | 2017-01-11 | 中国医学科学院整形外科医院 | Tissue engineering dynamic culture system adopting dynamic mechanical stimulation |
| CN114699971A (en) * | 2016-03-31 | 2022-07-05 | 环球生命科技咨询美国有限责任公司 | Magnetic mixer |
| US11969701B2 (en) | 2016-03-31 | 2024-04-30 | Global Life Sciences Solutions Usa Llc | Magnetic mixers |
-
2012
- 2012-03-15 CN CN 201220096358 patent/CN202465713U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114699971A (en) * | 2016-03-31 | 2022-07-05 | 环球生命科技咨询美国有限责任公司 | Magnetic mixer |
| US11969701B2 (en) | 2016-03-31 | 2024-04-30 | Global Life Sciences Solutions Usa Llc | Magnetic mixers |
| CN106085855A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
| CN106085856A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of combination type organizational project dynamic cultivation instrument |
| CN106318868A (en) * | 2016-08-22 | 2017-01-11 | 中国医学科学院整形外科医院 | Tissue engineering dynamic culture system adopting dynamic mechanical stimulation |
| CN106085855B (en) * | 2016-08-22 | 2019-01-15 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
| CN106318868B (en) * | 2016-08-22 | 2019-04-16 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument stimulated using dynamic mechanical |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121003 Termination date: 20150315 |
|
| EXPY | Termination of patent right or utility model |