CN106085855A - A kind of organizational project dynamic cultivation instrument - Google Patents
A kind of organizational project dynamic cultivation instrument Download PDFInfo
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- CN106085855A CN106085855A CN201610702394.4A CN201610702394A CN106085855A CN 106085855 A CN106085855 A CN 106085855A CN 201610702394 A CN201610702394 A CN 201610702394A CN 106085855 A CN106085855 A CN 106085855A
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- 239000000696 magnetic material Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 9
- 230000005611 electricity Effects 0.000 claims description 4
- 230000001276 controlling effect Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 19
- 230000008901 benefit Effects 0.000 description 8
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 239000012531 culture fluid Substances 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000010008 shearing Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000005307 ferromagnetism Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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Abstract
The present invention relates to a kind of organizational project dynamic cultivation instrument, unlike the prior art, including box body, drive motor, rotary support, attraction block, culture bottle and rotor；Described driving motor is placed in described tray interior, and its clutch end is the most upward；Described rotary support is fixing with the clutch end of described driving motor to be connected, and described attraction block is placed on described rotary support；Described culture bottle is placed in described cartridge upper surface, and is right against described driving motor at the bottom of bottle, and described rotor is put in described culture bottle；Described rotor is that magnetic material is made with described attraction block at least one, and both attract each other.This cultivation instrument is not only simple in structure, and cost is relatively low, and safe and reliable, and the suitability is higher.
Description
Technical field
The present invention relates to biomedical tissue engineering field, particularly relate to a kind of organizational project dynamic cultivation instrument.
Background technology
Tissue engineering, is one and combines with cytobiology and material science, carries out external or construct in vitro tissue
Or the new branch of science of organ.Its ultimate principle is to obtain a small amount of biological tissue from body, will with special enzyme or additive method
Cell (also known as seed cell) from tissue separate carry out in vitro cultivate amplification, then by the cell of amplification with have good
Good biocompatibility, degradability and absorbable biomaterial (support) mix according to a certain percentage, make cell adhesion give birth to
Cell-material composite is formed on thing material (support)；By tissue or the lesions position of this complex implanting to human body, along with
Biomaterial is the most gradually degraded and absorbs, and the cell of implantation is the most constantly bred and extracellular matrix secretion, finally
Form corresponding tissue or organ, thus reach to repair wound and the purpose of Reconstruction of The Function.
Organizational project development for many years makes people have successfully been obtained the tissue engineering bone/cartilage of various shape and purposes, but
Traditional cultural method mostly is static culture method.Its biomechanics characteristic of engineered tissue that static culture method is cultivated is poor,
Compressive property, the histological difference that elastic modelling quantity etc. is repaired with hope is bigger, it is impossible to realizes it and repairs target.For betterment works group
The mechanical property knitted, uses dynamic cultivation method, it is common that by the rotation of the culture fluid that stirring causes, Jin Erwei at present
Engineered tissue provides the stimulation of shearing force, can improve the biomechanics characteristic of engineered tissue.In carrying out dynamic cultivation, generally
Need to use the dynamic cultivation instrument with agitating function, be positioned in the incubator under gnotobasis and cultivate, but at present
Dynamic cultivation instrument one be that price is high, for the laboratory of domestic colleges and universities, it is impossible to realize bulk purchase；Two be volume relatively
Greatly, a standard culture case (internal diameter 46x46x48cm) at most puts 4, this instrument of single seat, 1, four these instruments, be for difference
Tissue gives different mechanical stimulation, then need the multiple incubator of multiple instrument, it is difficult to realize, when the culture apparatus of four uses,
The mixing speed at four seats is identical, it is impossible to meet experiment packet needs；Three is that base instrument cannot be carried out High Temperature High Pressure
Sterilization, is easily destroyed culture environment；Four is to use 220V Alternating Current Power Supply, after entirety inserts incubator, dangerous high, the diameter of wire
Greatly, and can only enter through incubator crack between a door and its frame, destroy airtight stable culture environment in incubator, accelerate incubator aging；Five
It is that the mode of the stirring to culture fluid typically realizes with the rotation of the stirring rod inserted in culture bottle or stirring vane, due to training
Support bottle long-pending general the most little, and be inserted into stirring rod or paddle sector-meeting makes the space in culture bottle narrower, with
As for accommodating bigger engineered tissue.
Summary of the invention
For solving at least one problem above-mentioned, the present invention provides a kind of organizational project dynamic cultivation instrument, and this cultivation instrument is not only
Simple in construction, cost is relatively low, and safe and reliable, and the suitability is higher.
The technical scheme is that a kind of organizational project dynamic cultivation instrument, with existing skill
Unlike art, including box body, drive motor, rotary support, attraction block, culture bottle and rotor；Described driving motor is placed in institute
Stating tray interior, its clutch end is the most upward；Described rotary support is fixing with the clutch end of described driving motor to be connected
Connecing, described attraction block is placed on described rotary support；Described culture bottle is placed in described cartridge upper surface, and is right against institute at the bottom of bottle
Stating driving motor, described rotor is put in described culture bottle；Described rotor and described attraction block at least one are magnetic material system
Become, and both attract each other.
The invention has the beneficial effects as follows, by the driving motor being placed in box body, bearing is rotated and rotates, and then drive
The attraction block being placed on rotary support rotates, and is put in the top being right against driving motor of box body, and bottle due to culture bottle again
Inside being placed with can be with the rotor attracting block to attract each other, so rotor can rotate in bottle along with the rotation attracting block, and then
The culture fluid in bottle is driven to rotate, it is achieved the engineered tissue in culture fluid to carry out the stimulation of shearing force, improves engineered tissue
Mechanical property.Whole apparatus structure is simple, with low cost, the most safe and reliable.
On the basis of technique scheme, the present invention can also do following improvement.
Further, also including the battery being placed in described box body, described driving motor is connected with described battery circuit, by institute
Stating battery is that described driving motor is powered.
Above-mentioned further scheme is used to provide the benefit that, with battery for driving motor to provide electric energy, simple and easy to get, cost
Cheap, box body can be put into easily simultaneously.
Further, described attraction block is button Magnet, and the rotation axis of described attraction block and described driving motor it
Between distance adjustable.Button Magnet is utilized to provide captivation to bottle internal rotor, simple and reliable, simultaneously because attract block to drive with described
Distance between the rotation axis of galvanic electricity machine is adjustable, can adjust the radius of gyration attracting block, to adapt to different size of cultivation
Bottle, can also meet the more parameter requirements in experiment simultaneously.
Further, described rotary support is that ferrimagnet is made, and described attraction block is adsorbed on described rotary support
Fixing, described rotary support is provided with groove, in described attraction block is placed in described groove and can in described groove near or
Direction away from rotation axis is moved.
Use above-mentioned further scheme to provide the benefit that, rotary support is provided with groove, attraction block can be made stable
Be placed on rotary support, and convenient regulation attracts the radius of gyration of block.
Further, also including supporting seat, described support seating is in described tray interior, and described driving motor is fixed on described
Support on seat.
Use above-mentioned further scheme to provide the benefit that, utilize the fixation supporting seat, it is ensured that drive motor
Stability during work, improves the using effect cultivating instrument.
Further, also include that on and off switch, described on and off switch are installed on the upper surface of described support seat, and drive with described
Dynamic motor circuit connects, for controlling the start and stop of described driving motor.
Use above-mentioned further scheme to provide the benefit that, by and off switch can realize easily the unlatching of instrument with
Close.
Further, circuit controller and knob, described driving motor, described knob the most described circuit controller electricity are also included
Road connects, and described circuit controller is placed in described tray interior, and described knob is installed on the upside of described support seat, described knob
The rotating speed of described driving motor is regulated by described circuit controller.
Above-mentioned further scheme is used to provide the benefit that, due in the incubation of engineered tissue, it is often necessary to no
Same shearing force stimulates, and is driven the rotating speed of motor by regulation, can obtain the different mixing speeds of culture fluid, and then obtain not
Same shearing force stimulates, and meets the cultivation demand of various engineered tissue.
Further, also include being installed on the electronic display on the upside of described support seat, described electronic display and described electricity
Road controller circuitry connects, for showing the rotating speed that described driving motor is current.
Above-mentioned further scheme is used to provide the benefit that, by driving current the turning of motor described in electronic display screen displays
Speed, in that context it may be convenient to the culture experiment of engineered tissue is carried out data record, and required turning can be adjusted to as required
Speed.
Further, described box body is that the material of high temperature high voltage resistant is made, and described box body includes upper cover and base, described upper cover
Hinged in side with described base.
Above-mentioned further scheme is used to provide the benefit that, before needing to be placed in aseptic incubator due to cultivation instrument, must
Must guarantee it is aseptic condition, need its disinfection, and disinfection way the most frequently used in laboratory is High Temperature High Pressure
Sterilization, so box body uses the material of high temperature high voltage resistant to make can carry out laboratory sterilization, simultaneously upper cover and base easily
After hinged, in that context it may be convenient to carry out folding.
Further, described box body top is provided with and protrudes from table on described box body with described culture bottle external diameter size adapts
The circular back-up ring in face, described back-up ring is centrally located on the rotation axis of described driving motor.
Above-mentioned further scheme is used to provide the benefit that, owing to culture bottle needs to be positioned on box body, in order to ensure training
The center supporting bottle is positioned on the rotation axis driving motor as far as possible, is provided with on box body top and described culture bottle external diameter size
The circular back-up ring protruding from described cartridge upper surface adapted, contributes to operator and places culture bottle accurately,
Have only to during placement put into back-up ring, i.e. can ensure that and aligned driving motor, and without looking in driving motor through upper cover
The heart, and once upper cover use be not transparent material, then be more difficult to carry out accurate and effective placement.
Accompanying drawing explanation
Fig. 1 is that the overall stereochemical structure of the specific embodiment of a kind of organizational project dynamic cultivation instrument provided by the present invention is shown
It is intended to；
Fig. 2 is the structural representation after opening upper cover in the specific embodiment shown in Fig. 1；
Fig. 3 is the structural representation after opening upper cover in the specific embodiment shown in Fig. 1 and removing front panel；
Fig. 4 is the facade structures schematic diagram of specific embodiment shown in Fig. 1；
Fig. 5 is the structural representation without culture bottle of specific embodiment shown in Fig. 1.
In accompanying drawing, the list of parts representated by each label is as follows:
Box body 1, drives motor 2, rotary support 3, attracts block 4, culture bottle 5, rotor 6, battery 7, supports seat 8, and power supply is opened
Pass 9, circuit controller 10, knob 11, electronic display 12, back-up ring 13, groove 31, upper cover 101, base 102.
Detailed description of the invention
Being described principle and the feature of the present invention below in conjunction with accompanying drawing, example is served only for explaining the present invention, and
Non-for limiting the scope of the present invention.
As shown in Figures 1 to 5, Fig. 1 is the specific embodiment of a kind of organizational project dynamic cultivation instrument provided by the present invention
Overall perspective view；Fig. 2 is the structural representation after opening upper cover in the specific embodiment shown in Fig. 1；Fig. 3 is figure
Structural representation after opening upper cover in specific embodiment shown in 1 and removing front panel；Fig. 4 is specific embodiment shown in Fig. 1
Facade structures schematic diagram；Fig. 5 is the structural representation after the removing culture bottle of specific embodiment shown in Fig. 1.
In the specific embodiment of a kind of organizational project dynamic cultivation instrument provided by the present invention, a kind of organizational project is dynamic
Cultivate instrument, including box body 1, drive motor 2, rotary support 3, attraction block 4, culture bottle 5 and rotor 6；Also include being placed in described box
Battery 7 in body 1, described driving motor 2 is connected with described battery 7 circuit, described battery 7 powers for described driving motor 2.
Described box body 1 is that the material of high temperature high voltage resistant is made, and the most medical high temperature high voltage resistant plastics, glass etc., described box body 1 includes
Lid 101 and base 102, described upper cover 101 is hinged in side with described base 102.Upper cover 101 can be made with transparent material,
So that inside is observed.
Described box body 1 top is provided with described box body 1 upper surface that protrudes from adapted with described culture bottle 5 external diameter size
Circular back-up ring 13, described back-up ring 13 is centrally located on the rotation axis of described driving motor 2.
Also including supporting seat 8, it is internal that described support seat 8 is placed in described box body 1, and described driving motor 2 is fixed on described
On support seat 8.Support seat 8 and can be set as board-like, and deck is in the top of described base 102, in order to the bottom for base 2 is empty
Between enough placing battery 7 parts such as grade.
It is internal that described driving motor 2 is placed in described box body 1, and its clutch end is the most upward；Described rotary support 3 and institute
Stating the fixing connection of clutch end driving motor 2, described attraction block 4 is placed on described rotary support 3；Described culture bottle 5 is put
In described box body 1 upper surface, and being right against described driving motor 2 at the bottom of bottle, described rotor 6 is put in described culture bottle 5；Described
Rotor 6 is that magnetic material is made with described attraction block 4 at least one, and both attract each other.
In a preferred embodiment, described attraction block 4 is button Magnet, and described rotary support 3 is ferromagnetism material
Material is made, and described attraction block 4 is adsorbed in be fixed on described rotary support 3, and described attraction block 4 and described driving motor 2
Rotation axis between distance adjustable.Rotor 6 is also made for magnetic material, can use the most more magnetic agitation
Son, outer layer is surrounded by anticorrosive, is shaped as spheroidal or capsule shape or rugby shape or other shape.In order to more square
Just regulation attracts the distance between block 4 and the rotation axis driving motor 2, can be provided with groove on described rotary support 3
31, in making described attraction block 4 be placed in described groove 31 and can be to the direction closer or far from rotation axis in described groove 31
Mobile.
In this specific embodiment, also include that on and off switch 9, described on and off switch 9 are installed on the upper table of described support seat 8
Face, for controlling the start and stop of described driving motor 2.Also include circuit controller 10 and knob 11, described driving motor 2, described
Knob 11 the most described circuit controller 10 circuit connects, and it is internal that described circuit controller 10 is placed in described box body 1, described knob 11
Being installed on the upside of described support seat 8, described knob 11 regulates turning of described driving motor 2 by described circuit controller 10
Speed.
In order to accurately obtain when the rotating speed of front wheel driving motor 2, supporting the electronic display 12 on the upside of seat 8, described electronics
Display screen 12 is connected with described circuit controller 10 circuit, for showing the rotating speed that described driving motor is current.
In this specific embodiment, box body 1 is in substantially rectangular structure, and length, width and height size is respectively 12x8x5cm, in training
Supporting in the use of bottle, the most all use 100 and 250ml two kinds of specifications, its height is respectively 99mm and 138mm, so
In the incubator of a 46x46x48cm, at least can place two-layer, place three row four row i.e. 12, amount at least for every layer
24 cultivation instrument can be placed, it is sufficient to meet the demand of experiment packet.
And owing to each cultivation instrument is independent control, required rotating speed can be set respectively, it is possible to the most right
Multiple cultivation instrument are respectively adopted different rotating speeds, for cultivating the mechanical stimulation that different destination organizations provides different, many to meet
The demand of individual experiment packet, and in prior art, four these instruments to give different mechanical stimulation for different tissues, then need
The multiple incubator of multiple instruments, can increase cost and inefficiency undoubtedly；And during the culture apparatus of four use, four seats
The mixing speed of position is identical, it is impossible to meet experiment packet needs.
It addition, box body can also be set as cylinder, structure is compacter, takies volume less.Box body may be designed in
Other variously-shaped such as ellipse, polygon etc., it will be appreciated that the variously-shaped spirit belonging to the present invention of box body and former
Then interior, should be included within the scope of the present invention.
In a kind of organizational project dynamic cultivation instrument provided by the present invention, owing to box body 1 and inner member are separate,
So in use, the device within box body 1 can individually be taken out and carry out disinfection by ultraviolet light, and box body 1 can carry out high temperature height
Pressure sterilization, then loads internal each device in base 102, mixes up the radius of gyration attracting block 4, power-on switch 9, regulation
Build upper cover 101 after rotating speed, then culture bottle is put in upper cover 101 back-up ring 13 above, can entirety put in incubator.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (10)
1. an organizational project dynamic cultivation instrument, it is characterised in that include box body (1), drive motor (2), rotary support (3),
Attract block (4), culture bottle (5) and rotor (6)；
It is internal that described driving motor (2) is placed in described box body (1), and its clutch end is the most upward；
Described rotary support (3) is fixing with the described clutch end of described driving motor (2) to be connected, and described attraction block (4) is solid
On described rotary support (3)；
Described culture bottle (5) is placed at the bottom of described box body (1) upper surface, and bottle and is right against described driving motor (2), described rotor
(6) it is put in described culture bottle (5)；
Described rotor (6) is that magnetic material is made with described attraction block (4) at least one, and both attract each other.
A kind of organizational project dynamic cultivation instrument the most according to claim 1, it is characterised in that also include being placed in described box body
(1) battery (7) in, described driving motor (2) is connected with described battery (7) circuit, described battery (7) be described driving electricity
Machine (2) is powered.
A kind of organizational project dynamic cultivation instrument the most according to claim 1, it is characterised in that described attraction block (4) is knob
Button distance between Magnet, and the rotation axis of described attraction block (4) and described driving motor (2) is adjustable.
A kind of organizational project dynamic cultivation instrument the most according to claim 3, it is characterised in that described rotary support (3) is
Ferrimagnet is made, and described attraction block (4) is adsorbed in described rotary support (3) and above fixes, and described rotary support sets on (3)
Fluted (31), described attraction block (4) is placed in described groove (31) and can be introversive closer or far from rotation at described groove (31)
The direction of shaft axis is moved.
A kind of organizational project dynamic cultivation instrument the most according to claim 1, it is characterised in that also include supporting seat (8), institute
Stating support seat (8) and be placed in described box body (1) inside, described driving motor (2) is fixed on described support seat (8).
A kind of organizational project dynamic cultivation instrument the most according to claim 5, it is characterised in that also include on and off switch (9),
Described on and off switch (9) is installed on the upper surface of described support seat (8), and is connected with described driving motor (2) circuit, is used for controlling
Make the start and stop of described driving motor (2).
A kind of organizational project dynamic cultivation instrument the most according to claim 6, it is characterised in that also include circuit controller
(10) and knob (11), described driving motor (2), described knob (11) the most described circuit controller (10) circuit connect, described
It is internal that circuit controller (10) is placed in described box body (1), and described knob (11) is installed on the upper surface of described support seat (8), institute
State knob (11) and regulated the rotating speed of described driving motor (2) by described circuit controller (10).
A kind of organizational project dynamic cultivation instrument the most according to claim 7, it is characterised in that also include being installed on described
The electronic display (12) of support seat (8) upside, described electronic display (12) is connected with described circuit controller (10) circuit, uses
In the rotating speed showing that described driving motor (2) is current.
9. according to a kind of organizational project dynamic cultivation instrument described in any one of claim 1 to 8, it is characterised in that described box body
(1) being that the material of high temperature high voltage resistant is made, described box body (1) includes upper cover (101) and base (102), and described upper cover (101) cuts with scissors
It is connected on described base (102).
10. according to a kind of organizational project dynamic cultivation instrument described in any one of claim 1 to 8, it is characterised in that described box body
(1) top is provided with the circular back-up ring protruding from described box body (1) upper surface adapted with described culture bottle (5) external diameter size
(13), described back-up ring (13) is centrally located on the rotation axis of described driving motor (2).
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| CN201610702394.4A CN106085855B (en) | 2016-08-22 | 2016-08-22 | A kind of organizational project dynamic cultivation instrument |
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