CN205933843U - Organizational project developments culture device - Google Patents
Organizational project developments culture device Download PDFInfo
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- CN205933843U CN205933843U CN201620917595.1U CN201620917595U CN205933843U CN 205933843 U CN205933843 U CN 205933843U CN 201620917595 U CN201620917595 U CN 201620917595U CN 205933843 U CN205933843 U CN 205933843U
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- box body
- motor
- organizational project
- instrument according
- dynamic cultivation
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Abstract
The utility model relates to an organizational project developments culture device, the different with prior art is, including box body, driving motor, rotatory support, attraction piece, blake bottle and rotor, driving motor arranges in inside the box body, its power take off end is up vertical, rotatory support with driving motor's power take off end fixed connection, attract the piece to arrange in on the rotatory support, the blake bottle is arranged in the box body upper surface to the bottle end just to driving motor, the rotor put in in the blake bottle, the rotor with attract piece at least to make for magnetic material to both inter attractions. This culture device is simple structure not only, and the cost is lower, safe and reliable moreover, and the suitability is stronger.
Description
Technical field
This utility model is related to biomedical tissue engineering field, more particularly, to a kind of organizational project dynamic cultivation instrument.
Background technology
Tissue engineering, is one and is combined with cytobiology and material science, carries out external or construct in vitro tissue
Or the new branch of science of organ.Its ultimate principle is to obtain a small amount of biological tissue from body, will with special enzyme or additive method
Cell (also known as seed cell) from tissue separate carry out in vitro culture amplification, then by the cell of amplification with have good
Good biocompatibility, degradability and absorbable biomaterial (support) mix according to a certain percentage, so that cell adhesion is being given birth to
Thing material (support) is upper to form cell-material composite；By the tissue of this complex implanting to human body or lesions position, with
Biomaterial is gradually degraded in vivo and absorbs, and the cell of implantation is constantly bred and extracellular matrix secretion, finally in vivo
Form corresponding tissue or organ, thus reaching the purpose repairing wound and Reconstruction of The Function.
Organizational project development for many years makes people have successfully been obtained the tissue engineering bone/cartilage of various shapes and purposes, but
Traditional cultural method mostly is static culture method.Its biomechanics characteristic of engineered tissue that static culture method is cultivated is poor,
Compressive property, elastic modelling quantity etc. larger with the histological difference of hope reparation it is impossible to realize its repair target.For betterment works group
The mechanical property knitted, the rotation of the culture fluid using dynamic cultivation method, causing typically by stirring at present more, Jin Erwei
Engineered tissue provides the stimulation of shearing force, can improve the biomechanics characteristic of engineered tissue.In carrying out dynamic cultivation, generally
Need, using the dynamic cultivation instrument with agitating function, to be positioned in the incubator under gnotobasiss and cultivated, but at present
Dynamic cultivation instrument one be that price is high, it is impossible to realize bulk purchase for the laboratory of domestic colleges and universities；Two be volume relatively
Greatly, a standard culture case (internal diameter 46x46x48cm) at most puts 4, this instrument of single seat, 1, four this instruments, be for difference
Tissue gives different mechanical stimulations, then need the multiple incubator of multiple instruments it is difficult to realize, when the culture apparatus of four use,
The mixing speed at four seats is identical it is impossible to meet experiment packet needs；Three is that base instrument cannot be carried out High Temperature High Pressure
Sterilization, is easily destroyed culture environment；Four is using 220V Alternating Current Power Supply, after integrally inserting incubator, dangerous high, the diameter of wire
Enter can only greatly, and through incubator crack between a door and its frame, destroy airtight stable culture environment in incubator, accelerate incubator aging；Five
It is that the mode of the stirring to culture fluid is typically realized with the stirring rod in insertion culture bottle or the rotation of stirring vane, due to training
Foster bottle amasss general all little, and is inserted into stirring rod or paddle sector-meeting makes the space in culture bottle narrower, with
As for larger engineered tissue cannot be accommodated.
Utility model content
For solving at least one problem above-mentioned, this utility model provides a kind of organizational project dynamic cultivation instrument, this culture instrument
It is not only simple in structure, cost is relatively low, and safe and reliable, and the suitability is higher.
The technical scheme that this utility model solves above-mentioned technical problem is as follows：A kind of organizational project dynamic cultivation instrument, with now
Have unlike technology, including box body, motor, rotary support, attract block, culture bottle and rotor；Described motor is put
In described tray interior, its clutch end is vertically upward；Described rotary support is solid with the clutch end of described motor
Fixed connection, described attraction block is placed on described rotary support；Described culture bottle is placed in described cartridge upper surface, and bottom of bottle is just right
In described motor, described rotor is put in described culture bottle；Described rotor with described attraction block at least one be magnetic material
Material is made, and both attract each other.
The beneficial effects of the utility model are, by the motor being placed in box body, bearing rotation are rotated, and then
Drive the attraction block rotation being placed on rotary support, and because culture bottle is put in the top being right against motor of box body, and
And in bottle, it is placed with the rotor that can attract each other with attraction block, so rotor can rotate in bottle with the rotation attracting block,
And then the culture fluid in drive bottle rotates, realize the engineered tissue in culture fluid is carried out with the stimulation of shearing force, improve engineering
The mechanical property of tissue.Whole device structure is simple, with low cost, simultaneously safe and reliable.
On the basis of technique scheme, this utility model can also do following improvement.
Further, also include the battery that is placed in described box body, described motor is connected with described battery circuit, by institute
State battery to power for described motor.
Beneficial effect using above-mentioned further scheme is to provide electric energy, simple and easy to get, cost with battery for motor
Cheap, can easily put in box body simultaneously.
Further, described attraction block is button Magnet, and the described rotation axiss attracting block and described motor it
Between distance adjustable.There is provided captivation using button Magnet to bottle internal rotor, simple and reliable, simultaneously because attracting block and described drive
The distance between rotation axiss of galvanic electricity machine are adjustable, can adjust the radius of gyration attracting block, to adapt to different size of culture
Bottle, can also meet the more parameter requirements in experiment simultaneously.
Further, described rotary support is made up of ferrimagnet, and described attraction block is adsorbed on described rotary support
Fixing, described rotary support is provided with groove, described attraction block be placed in described groove and can in described groove near or
Direction away from rotation axiss is moved.
Beneficial effect using above-mentioned further scheme is to be provided with groove on rotary support, and attraction block can be made stable
Be placed on rotary support, and convenient adjust the radius of gyration attracting block.
Further, also include support base, described support base is placed in described tray interior, described motor is fixed on described
On support base.
Beneficial effect using above-mentioned further scheme is, using support base fixation it is ensured that motor
Stability during work, improves the using effect of culture instrument.
Further, also include on and off switch, described on and off switch is installed on the upper surface of described support base, and with described drive
Dynamic motor circuit connects, for controlling the start and stop of described motor.
Beneficial effect using above-mentioned further scheme is, by and off switch can easily realize the unlatching of instrument with
Close.
Further, circuit controller and knob are also included, all described circuit controller electricity of described motor, described knob
Road connects, and described circuit controller is placed in described tray interior, and described knob is installed on the upside of described support base, described knob
Adjust the rotating speed of described motor by described circuit controller.
Beneficial effect using above-mentioned further scheme is, due in the incubation of engineered tissue it is often necessary to not
Same shearing force stimulates, and by adjusting the rotating speed of motor, can obtain the different mixing speeds of culture fluid, and then obtain not
Same shearing force stimulates, and meets the culture demand of various engineered tissues.
Further, also include being installed on the electronic display on the upside of described support base, described electronic display and described electricity
Road controller circuitry connects, the rotating speed current for showing described motor.
Beneficial effect using above-mentioned further scheme is, by current the turning of motor described in electronic display screen displays
Speed is in that context it may be convenient to carry out data record to the culture experiment of engineered tissue, and can be adjusted to required turning as needed
Speed.
Further, described box body is made up of the material of high temperature high voltage resistant, and described box body includes lid and bottom seat, described upper lid
Hinged in side with described base.
Beneficial effect using above-mentioned further scheme is, before needing to be placed in aseptic incubator due to culture instrument, must
Must guarantee it is aseptic condition, need to its disinfection, and the most frequently used disinfection way in laboratory is High Temperature High Pressure
Sterilization, so box body is made and can easily be carried out laboratory sterilization using the material of high temperature high voltage resistant, goes up lid and bottom seat simultaneously
In that context it may be convenient to carry out folding after hinged.
Further, described box body top be provided with described culture bottle external diameter size be adapted protrude from described box body upper table
The circular back-up ring in face, described back-up ring is centrally located on the rotation axiss of described motor.
Beneficial effect using above-mentioned further scheme is, because culture bottle needs to be positioned on box body, in order to ensure training
The center of foster bottle is located on the rotation axiss of motor as far as possible, is provided with and described culture bottle external diameter size on box body top
The adaptable circular back-up ring protruding from described cartridge upper surface, contributes to operator and is accurately placed culture bottle,
Only need to during placement put in back-up ring, you can guarantee to have aligned motor, and need not look in motor through upper lid
The heart, and once upper lid employing is not transparent material, then be more difficult to carry out accurate and effective placement.
Brief description
Fig. 1 is a kind of overall three-dimensional knot of the specific embodiment of organizational project dynamic cultivation instrument provided by the utility model
Structure schematic diagram；
Fig. 2 is the structural representation after opening lid in the specific embodiment shown in Fig. 1；
Fig. 3 is to open the structural representation after covering and remove front panel in the specific embodiment shown in Fig. 1；
Fig. 4 is the facade structures schematic diagram of specific embodiment shown in Fig. 1；
Fig. 5 is the structural representation of the no culture bottle of specific embodiment shown in Fig. 1.
In accompanying drawing, the list of parts representated by each label is as follows：
Box body 1, motor 2, rotary support 3, attract block 4, culture bottle 5, rotor 6, battery 7, support base 8, power supply is opened
Pass 9, circuit controller 10, knob 11, electronic display 12, back-up ring 13, groove 31, upper lid 101, base 102.
Specific embodiment
Below in conjunction with accompanying drawing, principle of the present utility model and feature are described, example is served only for explaining this practicality
New, it is not intended to limit scope of the present utility model.
As shown in Figures 1 to 5, Fig. 1 is a kind of concrete reality of organizational project dynamic cultivation instrument provided by the utility model
Apply the entirety perspective view of example；Fig. 2 is the structural representation after opening lid in the specific embodiment shown in Fig. 1；Fig. 3
The structural representation after covering and remove front panel is opened in specific embodiment shown in Fig. 1；Fig. 4 is specifically real shown in Fig. 1
Apply the facade structures schematic diagram of example；Fig. 5 is the structural representation after the removing culture bottle of specific embodiment shown in Fig. 1.
In a kind of specific embodiment of organizational project dynamic cultivation instrument provided by the utility model, a kind of organizational project
Dynamic cultivation instrument, including box body 1, motor 2, rotary support 3, attracts block 4, culture bottle 5 and rotor 6；Also include being placed in institute
State the battery 7 in box body 1, described motor 2 is connected with described battery 7 circuit, be described motor 2 by described battery 7
Power supply.Described box body 1 is made up of the material of high temperature high voltage resistant, such as medical high temperature high voltage resistant plastics, glass etc., described box body 1
Including on lid 101 and base 102, described upper lid 101 is hinged in side with described base 102.Upper lid 101 can use transparent material
Make, in order to observe to inside.
Described box body 1 top be provided be adapted with described culture bottle 5 external diameter size protrude from described box body 1 upper surface
Circular back-up ring 13, described back-up ring 13 is centrally located on the rotation axiss of described motor 2.
Also include support base 8, described support base 8 is placed in inside described box body 1, described motor 2 is fixed on described
On support seat 8.Support base 8 can be set as board-like, and deck is in the top of described base 102, empty in order to the bottom for base 2
Between enough placing battery 7 grade parts.
Described motor 2 is placed in inside described box body 1, and its clutch end is vertically upward；Described rotary support 3 and institute
The clutch end stating motor 2 is fixedly connected, and described attraction block 4 is placed on described rotary support 3；Described culture bottle 5 is put
In described box body 1 upper surface, and bottom of bottle is right against described motor 2, and described rotor 6 is put in described culture bottle 5；Described
Rotor 6 and described attraction block 4 at least one be made up of magnetic material, and both attract each other.
In a preferred embodiment, described attraction block 4 is button Magnet, and described rotary support 3 is ferromagnetism material
Material is made, and described attraction block 4 is adsorbed on described rotary support 3 to be fixed, and described attraction block 4 and described motor 2
The distance between rotation axiss adjustable.Rotor 6 is also made up of magnetic material, can be using magnetic agitation more in the market
Son, outer layer is surrounded by anticorrosive, is shaped as spheroidal or capsule shape or rugby shape or other shapes.In order to more square
Just regulation attracts the distance between rotation axiss of block 4 and motor 2, can be provided with groove on described rotary support 3
31, so that described attraction block 4 is placed in described groove 31 and can be to the direction closer or far from rotation axiss in described groove 31
Mobile.
In this specific embodiment, also include on and off switch 9, described on and off switch 9 is installed on the upper table of described support base 8
Face, for controlling the start and stop of described motor 2.Also include circuit controller 10 and knob 11, described motor 2, described
All described circuit controller 10 circuit of knob 11 connects, and described circuit controller 10 is placed in inside described box body 1, described knob 11
It is installed on the upside of described support base 8, described knob 11 adjusts turning of described motor 2 by described circuit controller 10
Speed.
In order to accurately obtain the rotating speed of current motor 2, in the electronic display 12 of support base 8 upside, described electronics
Display screen 12 is connected with described circuit controller 10 circuit, the rotating speed current for showing described motor.
In this specific embodiment, box body 1 is respectively 12x8x5cm in substantially rectangular structure, length, width and height size, in training
In the use of foster bottle, in most cases all using 100 and 250ml two kinds of specifications, its height is respectively 99mm and 138mm, so
In the incubator of a 46x46x48cm, at least can place two-layer, place three row four row i.e. 12, altogether at least for every layer
24 culture instrument can be placed it is sufficient to meet the demand of experiment packet.
And because each culture instrument is independent control, required rotating speed can be set respectively it is possible to simultaneously right
Multiple culture instrument are respectively adopted different rotating speeds, provide different mechanical stimulations for cultivating different destination organizations, many to meet
The demand of individual experiment packet, and in prior art, four this instruments will give different mechanical stimulations for different tissues, then need
The multiple incubator of multiple instrument, undoubtedly can increase cost and inefficiency；And during the culture apparatus of four use, four seats
The mixing speed of position is identical it is impossible to meet experiment packet needs.
In addition, box body can also be set as cylinder, structure is compacter, takies volume less.Box body may be designed in
Other variously-shaped such as ellipse, polygons etc. are it will be appreciated that the variously-shaped of box body belongs to spirit of the present utility model
Only interior with principle, should be included within protection domain of the present utility model.
In a kind of organizational project dynamic cultivation instrument provided by the utility model, because box body 1 and inner member are mutual
Independent, so when using, the device within box body 1 can individually be taken out and carry out disinfection by ultraviolet light, and box body 1 can carry out height
Warm autoclave sterilization, then loads internal each device in base 102, mixes up the radius of gyration attracting block 4, power-on switchs 9,
Cover 101 after adjusting rotating speed, then culture bottle is put in the back-up ring 13 above upper lid 101, you can entirety puts into incubator
In.
The foregoing is only preferred embodiment of the present utility model, not in order to limit this utility model, all in this practicality
Within new spirit and principle, any modification, equivalent substitution and improvement made etc., should be included in guarantor of the present utility model
Within the scope of shield.
Claims (10)
1. a kind of organizational project dynamic cultivation instrument it is characterised in that include box body (1), motor (2), rotary support (3),
Attract block (4), culture bottle (5) and rotor (6)；
Described motor (2) is placed in described box body (1) inside, and its clutch end is vertically upward；
Described rotary support (3) is fixedly connected with the described clutch end of described motor (2), and described attraction block (4) is solid
On described rotary support (3)；
Described culture bottle (5) is placed in described box body (1) upper surface, and bottom of bottle is right against described motor (2), described rotor
(6) it is put in described culture bottle (5)；
Described rotor (6) and described attraction block (4) at least one be made up of magnetic material, and both attract each other.
2. a kind of organizational project dynamic cultivation instrument according to claim 1 is it is characterised in that also include being placed in described box body
(1) battery (7) in, described motor (2) is connected with described battery (7) circuit, is described driving electricity by described battery (7)
Machine (2) is powered.
3. a kind of organizational project dynamic cultivation instrument according to claim 1 is it is characterised in that described attraction block (4) is knob
Button Magnet, and described attraction block (4) is adjustable with the distance between rotation axiss of described motor (2).
4. a kind of organizational project dynamic cultivation instrument according to claim 3 is it is characterised in that described rotary support (3) is
Ferrimagnet is made, and described attraction block (4) is adsorbed in described rotary support (3) and above fixes, and described rotary support (3) sets
Fluted (31), described attraction block (4) is placed in described groove (31) and can be in described groove (31) introversion closer or far from rotation
The direction of shaft axis is moved.
5. a kind of organizational project dynamic cultivation instrument according to claim 1 is it is characterised in that also include support base (8), institute
State support base (8) and be placed in described box body (1) inside, described motor (2) is fixed on described support base (8).
6. a kind of organizational project dynamic cultivation instrument according to claim 5 is it is characterised in that also include on and off switch (9),
Described on and off switch (9) is installed on the upper surface of described support base (8), and is connected with described motor (2) circuit, for controlling
Make the start and stop of described motor (2).
7. a kind of organizational project dynamic cultivation instrument according to claim 6 is it is characterised in that also include circuit controller
(10) and knob (11), all described circuit controller (10) circuit of described motor (2), described knob (11) connects, described
Circuit controller (10) is placed in described box body (1) inside, and described knob (11) is installed on the upper surface of described support base (8), institute
State the rotating speed that knob (11) adjusts described motor (2) by described circuit controller (10).
8. a kind of organizational project dynamic cultivation instrument according to claim 7 is it is characterised in that also include being installed on described
Electronic display (12) on the upside of support seat (8), described electronic display (12) is connected with described circuit controller (10) circuit, uses
In the current rotating speed of the described motor of display (2).
9. a kind of organizational project dynamic cultivation instrument according to any one of claim 1 to 8 is it is characterised in that described box body
(1) it is made up of the material of high temperature high voltage resistant, lid (101) and base (102) in the inclusion of described box body (1), described upper lid (101) hinge
It is connected on described base (102).
10. a kind of organizational project dynamic cultivation instrument according to any one of claim 1 to 8 is it is characterised in that described box body
(1) top is provided with the circular back-up ring protruding from described box body (1) upper surface being adapted with described culture bottle (5) external diameter size
(13), described back-up ring (13) is centrally located on the rotation axiss of described motor (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620917595.1U CN205933843U (en) | 2016-08-22 | 2016-08-22 | Organizational project developments culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620917595.1U CN205933843U (en) | 2016-08-22 | 2016-08-22 | Organizational project developments culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205933843U true CN205933843U (en) | 2017-02-08 |
Family
ID=57947527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620917595.1U Expired - Fee Related CN205933843U (en) | 2016-08-22 | 2016-08-22 | Organizational project developments culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205933843U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085855A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
-
2016
- 2016-08-22 CN CN201620917595.1U patent/CN205933843U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085855A (en) * | 2016-08-22 | 2016-11-09 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
| CN106085855B (en) * | 2016-08-22 | 2019-01-15 | 中国医学科学院整形外科医院 | A kind of organizational project dynamic cultivation instrument |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170208 Termination date: 20200822 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |