CN215627884U - Dynamic mechanical stimulation culture device - Google Patents
Dynamic mechanical stimulation culture device Download PDFInfo
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- CN215627884U CN215627884U CN202121512225.7U CN202121512225U CN215627884U CN 215627884 U CN215627884 U CN 215627884U CN 202121512225 U CN202121512225 U CN 202121512225U CN 215627884 U CN215627884 U CN 215627884U
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Abstract
The utility model belongs to the technical field of biomedicine, and particularly relates to a dynamic mechanical stimulation culture device which comprises a base, a motor, a rotary disc, a culture dish, a rotary shaft and a telescopic plate, wherein the motor is arranged inside the base and is connected with the rotary disc; the utility model discloses a culture device, which comprises a rotating shaft, a telescopic plate, a culture dish and a top plate, wherein the top plate is arranged at the top of the rotating shaft, the rotating shaft is provided with a through groove, one end of the telescopic plate penetrates through the through groove to be connected with the rotating shaft, and the other end of the telescopic plate is connected with the culture dish.
Description
Technical Field
The utility model belongs to the technical field of biomedicine, and particularly relates to a dynamic mechanical stimulation culture device.
Background
In recent years, clinical medicine has been developed to step into a new stage of "regenerative medicine", i.e., tissue engineering, with the development of cell culture technology, transplantation technology, and biomaterial technology. The tissue engineering is to inoculate relevant tissue cells cultured in vitro on a biological material with excellent compatibility and degradability to form a cell-material composite scaffold, then implant the cell-material composite scaffold into the damaged tissue part, and when the biological material carrying cells is degraded and absorbed by an organism along with time, the cells are continuously proliferated, migrated and differentiated to form new tissues, thereby achieving the purposes of tissue repair and function reconstruction. The main influencing factors for constructing the functional tissue engineering scaffold comprise seed cells, scaffold materials, external stimulation and the like. The in vitro functional culture of the cells is the key of tissue engineering research and is also the technical basis for applying the scaffold to clinic. Meanwhile, the in vitro cell survival conditions similar to the in vivo conditions are provided for the culture and the functionalization of the tissue engineering scaffold, wherein the physical factors such as mechanics, electricity and the like play a non-negligible role.
Cell culture technology is an important method for research in cell biology, molecular biology and the like. The study of cell function, metabolism and response to environmental factors requires a condition that can both isolate cells from the direct effects of a complex environment and maintain normal vital activities. This condition is established without isolation of the medium and the cell culture chamber. The nutrient environment for cell culture can be provided by the culture medium, and the environmental parameters for cell culture can be provided by the cell culture box. The conventional cell culture box can accurately provide temperature, humidity and gas environment required by cell culture, has stable performance, quick response and high reliability, and can effectively prevent the pollution to cultured cells. But none provide a microenvironment that mimics in vivo mechanics.
The mechanical microenvironment has important influence on the development and regeneration of organ tissues and the life activities of cells at each level, such as proliferation, migration, differentiation, apoptosis and the like. For example: the tendon is an important tissue connecting bones and muscles, and can continuously receive the action of mechanical load in the in vivo environment of the tendon, and the growth, development and regeneration of the tendon are closely related to mechanical stimulation. The biological effects and effects of mechanical stimulation have been studied in various cells and tissues, and it has been shown that tendon stimulation of different degrees can induce tendon stem cells to differentiate in different directions, mechanical stimulation of low intensity can promote proliferation of stem cells and differentiation of cells towards tendon, and mechanical stimulation of high intensity can lead to differentiation of stem cells towards fat, bone and cartilage.
Chinese patent application No. CN201620919052.3 discloses a tissue engineering dynamic culture apparatus using dynamic mechanical stimulation, which comprises one or more culture apparatus units, wherein each culture apparatus unit comprises a box body, a driving motor, a controller, a rotary support, an attraction block, a culture bottle and a rotor; the culture bottle is arranged above the box body, the bottom of the culture bottle faces the driving motor, and the rotor is placed in the culture bottle; at least one of the rotor and the attraction block is made of a magnetic material, and the rotor and the attraction block attract each other; the controller is connected with the driving motor circuit and is provided with a speed control module which can control the driving motor to work according to a set rotating speed. The culture instrument can not only realize the stimulation of general continuous constant shearing force, but also generate circularly changed stirring speed through program setting, provide the stimulation of circular dynamic shearing force for the cultured engineering tissue and further improve the culture effect of the engineering tissue.
However, the culture device in the above prior art can only provide a single mechanical stimulation in one culture process, and the biological tissues cultured in the same culture device are subjected to the same mechanical stimulation, which does not play a control role, so that a plurality of culture devices are required to set different parameters for culturing the biological tissues, which is time-consuming and labor-consuming.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects in the prior art, the utility model provides a dynamic mechanical stimulation culture device, which is used for solving the problems that the conventional culture device can only provide single mechanical stimulation in one culture process, and biological tissues cultured in the same culture device are subjected to the same mechanical stimulation and cannot play a contrast role, so that a plurality of culture devices are required to set different parameters to culture the biological tissues, and time and labor are consumed.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the culture dish rack comprises a base, a motor, a rotary table, a culture dish, a rotary shaft and a telescopic plate, wherein the motor is arranged inside the base and is connected with the rotary table, the rotary table is connected with the rotary shaft, and the culture dish is placed on the rotary table;
the top plate is installed at the top of the rotating shaft, the rotating shaft is provided with a through groove, one end of the expansion plate penetrates through the through groove to be connected with the rotating shaft, and the other end of the expansion plate is connected with the culture dish.
Culture solution and cultured biological tissues are filled in the culture dish, the motor rotates to drive the rotary disc to rotate, the rotary disc drives the rotary shaft to rotate, and the rotation of the rotary disc and the rotary shaft provides mechanical stimulation for the biological tissues; because the length of the fixed connection between the expansion plate and the rotating shaft is different, the rotating radius of the culture dish on the expansion plate is determined to be different, and the centripetal force is further different, so that different mechanical stimuli can be provided in the same culture device; specifically, the telescopic plate is inserted into the through groove, the inserting depth determines the rotating radius of the culture dish on the telescopic plate, the deeper the insertion is, the smaller the rotating radius is, and then the telescopic plate is fixed by the bolt rod.
Further, the top plate is provided with a through hole, the expansion plate is provided with a plug pin hole, and the diameter of the plug pin hole is equal to that of the through hole.
Be equipped with a plurality of bolt holes on the expansion plate, the expansion plate of being convenient for still can porously fix after passing through the groove, and the through-hole mainly used fixes the bolt pole.
The telescopic plate is characterized by further comprising a bolt rod, the length of the bolt rod is longer than that of the rotating shaft, and the bolt rod penetrates through the through hole and the bolt hole to fixedly connect the telescopic plate with the rotating shaft.
The bolt rod firstly penetrates through the through hole and then penetrates through the bolt hole, and both sides of the expansion plate penetrating through the through groove are fixed by the bolt rod.
Furthermore, a fixing seat is arranged at one end, without the plug pin hole, of the telescopic plate, and the culture dish is connected with the fixing seat.
Furthermore, the number of the expansion plates is six, the number of the through grooves is also six, and the six through grooves are spirally distributed on the rotating shaft.
Further, still include the case lid, case lid and base swing joint.
After all the culture dishes and the expansion plates are installed, the box cover is covered to protect the equipment.
Compared with the prior art, the utility model has the following beneficial effects:
1. mechanical stimulation is provided by rotating the rotating shaft and the rotating disc, and the mechanical stimulation with different sizes is realized by setting the connection length of the telescopic plate;
2. the same culture device can culture a plurality of biological tissues and provide different mechanical stimulation for each biological tissue, a plurality of culture devices are not needed for testing, and the efficiency is improved.
Drawings
FIG. 1 is a schematic perspective view of an embodiment of a dynamic mechanical stimulation culture apparatus according to the present invention;
FIG. 2 is a schematic diagram of a right side view of an embodiment of the dynamic mechanical stimulation culture apparatus of the present invention;
FIG. 3 is a cross-sectional view A-A of FIG. 2;
FIG. 4 is a schematic perspective view of a case cover in an embodiment of the dynamic mechanical stimulation culture apparatus according to the present invention;
reference numerals in the drawings of the specification include:
the culture dish comprises a base 1, a box cover 2, a motor 3, a rotary disc 4, a culture dish 5, a rotary shaft 6, a through groove 601, a telescopic plate 7, a fixed seat 701, a bolt hole 702, a top disc 8, a through hole 801 and a bolt rod 9.
Detailed Description
In order that those skilled in the art can better understand the present invention, the following technical solutions are further described with reference to the accompanying drawings and examples.
Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "inner", "outer", etc. are used for indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not indicated or implied that the referred device or element must have a specific orientation, be constructed in a specific orientation and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limitations of the present patent, and the specific meanings of the terms may be understood by those skilled in the art according to specific situations.
In the description of the present invention, unless otherwise explicitly specified or limited, the term "connected" or the like, if appearing to indicate a connection relationship between the components, is to be understood broadly, for example, as being fixed or detachable or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through one or more other components or may be in an interactive relationship with one another. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The first embodiment is as follows:
as shown in fig. 1-4, a dynamic mechanical stimulation culture device comprises a base 1, a motor 3, a turntable 4, a culture dish 5, a rotating shaft 6 and a retractable plate 7, wherein the motor 3 is installed inside the base 1, the motor 3 is connected with the turntable 4, the turntable 4 is connected with the rotating shaft 6, and the culture dish 5 is placed on the turntable 4;
Culture solution and cultured biological tissues are filled in the culture dish 5, the motor 3 rotates to drive the rotary disc 4 to rotate, the rotary disc 4 drives the rotary shaft 6 to rotate, and the rotation of the rotary disc 4 and the rotary shaft 6 provides mechanical stimulation for the biological tissues; because the length of the fixed connection between the expansion plate 7 and the rotating shaft 6 is different, the rotating radius of the culture dish 5 on the expansion plate 7 is determined to be different, and the centripetal force is different, so that different mechanical stimuli can be provided in the same culture device; specifically, the extension plate 7 is inserted into the through groove 601, the depth of the insertion determines the radius of rotation of the culture dish 5 on the extension plate 7, the deeper the insertion, the smaller the radius of rotation, and then the latch rod 9 is used to fix the extension plate 7.
The top disk 8 is provided with a through hole 801, the telescopic plate 7 is provided with a pin hole 702, and the diameter of the pin hole 702 is equal to that of the through hole 801.
The telescopic plate 7 is provided with a plurality of bolt holes 702, so that the telescopic plate 7 can be fixed in a hole after penetrating through the through groove 601, and the through holes 801 are mainly used for fixing the bolt rods 9.
Example two:
as a further improvement of the previous embodiment, as shown in fig. 1-4, a dynamic mechanical stimulation culture apparatus includes a base 1, a motor 3, a turntable 4, a culture dish 5, a rotation shaft 6, and a retractable plate 7, wherein the motor 3 is installed inside the base 1, the motor 3 is connected with the turntable 4, the turntable 4 is connected with the rotation shaft 6, and the culture dish 5 is placed on the turntable 4;
Culture solution and cultured biological tissues are filled in the culture dish 5, the motor 3 rotates to drive the rotary disc 4 to rotate, the rotary disc 4 drives the rotary shaft 6 to rotate, and the rotation of the rotary disc 4 and the rotary shaft 6 provides mechanical stimulation for the biological tissues; because the length of the fixed connection between the expansion plate 7 and the rotating shaft 6 is different, the rotating radius of the culture dish 5 on the expansion plate 7 is determined to be different, and the centripetal force is different, so that different mechanical stimuli can be provided in the same culture device; specifically, the extension plate 7 is inserted into the through groove 601, the depth of the insertion determines the radius of rotation of the culture dish 5 on the extension plate 7, the deeper the insertion, the smaller the radius of rotation, and then the latch rod 9 is used to fix the extension plate 7.
The top disk 8 is provided with a through hole 801, the telescopic plate 7 is provided with a pin hole 702, and the diameter of the pin hole 702 is equal to that of the through hole 801.
The telescopic plate 7 is provided with a plurality of bolt holes 702, so that the telescopic plate 7 can be fixed in a hole after penetrating through the through groove 601, and the through holes 801 are mainly used for fixing the bolt rods 9.
The telescopic plate is characterized by further comprising a latch rod 9, the length of the latch rod 9 is longer than that of the rotating shaft 6, and the latch rod 9 penetrates through the through hole 801 and the latch hole 702 to fixedly connect the telescopic plate 7 and the rotating shaft 6.
The end of the expansion plate 7 without the plug hole 702 is provided with a fixed seat 701, and the culture dish 5 is connected with the fixed seat 701.
The number of the expansion plates 7 is six, the number of the through grooves 601 is also six, and the six through grooves 601 are spirally distributed on the rotating shaft 6.
Still include case lid 2, case lid 2 and base 1 swing joint.
In the second embodiment, compared with the first embodiment, the plug pin rod 9 in the second embodiment firstly passes through the through hole 801 and then passes through the plug pin hole 702, both sides of the expansion plate 7 passing through the through groove 601 are fixed by the plug pin rod 9, and after all the culture dishes 5 and the expansion plates 7 are installed, the box cover 2 is covered to protect the equipment.
The use method of the device comprises the following steps:
culture solution and cultured biological tissues are filled in the culture dish 5, the motor 3 rotates to drive the rotary disc 4 to rotate, the rotary disc 4 drives the rotary shaft 6 to rotate, and the rotation of the rotary disc 4 and the rotary shaft 6 provides mechanical stimulation for the biological tissues; because the length of the fixed connection between the expansion plate 7 and the rotating shaft 6 is different, the rotating radius of the culture dish 5 on the expansion plate 7 is determined to be different, and the centripetal force is different, so that different mechanical stimuli can be provided in the same culture device; specifically, the extension plate 7 is inserted into the through groove 601, the depth of the insertion determines the radius of rotation of the culture dish 5 on the extension plate 7, the deeper the insertion, the smaller the radius of rotation, and then the latch rod 9 is used to fix the extension plate 7.
The foregoing are merely exemplary embodiments of the present invention, and no attempt is made to show structural details of the utility model in more detail than is necessary for the fundamental understanding of the art, the description taken with the drawings making apparent to those skilled in the art how the several forms of the utility model may be embodied in practice with the teachings of the utility model. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent.
Claims (6)
1. A dynamic mechanical stimulation culture device is characterized in that: the automatic culture dish fixing device comprises a base (1), a motor (3), a rotary table (4), a culture dish (5), a rotary shaft (6) and a telescopic plate (7), wherein the motor (3) is installed inside the base (1), the motor (3) is connected with the rotary table (4), the rotary table (4) is connected with the rotary shaft (6), and the culture dish (5) is placed on the rotary table (4);
top plate (8) are installed at pivot (6) top, pivot (6) are equipped with logical groove (601), expansion plate (7) one end is passed logical groove (601) and is connected with pivot (6), the other end and the culture dish (5) of expansion plate (7) are connected.
2. The dynamic mechanical stimulation culture device of claim 1, wherein: the top disc (8) is provided with a through hole (801), the expansion plate (7) is provided with a plug pin hole (702), and the diameter of the plug pin hole (702) is equal to that of the through hole (801).
3. The dynamic mechanical stimulation culture device of claim 2, wherein: the telescopic plate is characterized by further comprising a bolt rod (9), the length of the bolt rod (9) is longer than that of the rotating shaft (6), and the bolt rod (9) penetrates through the through hole (801) and the bolt hole (702) to fixedly connect the telescopic plate (7) and the rotating shaft (6).
4. The dynamic mechanical stimulation culture device of claim 3, wherein: and a fixing seat (701) is arranged at one end, without the plug pin hole (702), of the expansion plate (7), and the culture dish (5) is connected with the fixing seat (701).
5. The dynamic mechanical stimulation culture device of claim 4, wherein: the number of the expansion plates (7) is six, the number of the through grooves (601) is also six, and the six through grooves (601) are spirally distributed on the rotating shaft (6).
6. The dynamic mechanical stimulation culture device of claim 5, wherein: still include case lid (2), case lid (2) and base (1) swing joint.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121512225.7U CN215627884U (en) | 2021-07-05 | 2021-07-05 | Dynamic mechanical stimulation culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121512225.7U CN215627884U (en) | 2021-07-05 | 2021-07-05 | Dynamic mechanical stimulation culture device |
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| Publication Number | Publication Date |
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| CN215627884U true CN215627884U (en) | 2022-01-25 |
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| CN202121512225.7U Active CN215627884U (en) | 2021-07-05 | 2021-07-05 | Dynamic mechanical stimulation culture device |
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