CN108728333A - A kind of biotechnology cell incubator and its cultural method - Google Patents
A kind of biotechnology cell incubator and its cultural method Download PDFInfo
- Publication number
- CN108728333A CN108728333A CN201810397180.XA CN201810397180A CN108728333A CN 108728333 A CN108728333 A CN 108728333A CN 201810397180 A CN201810397180 A CN 201810397180A CN 108728333 A CN108728333 A CN 108728333A
- Authority
- CN
- China
- Prior art keywords
- babinet
- cell
- case lid
- supporting plate
- rotating disk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 claims abstract description 30
- 238000002347 injection Methods 0.000 claims abstract description 11
- 239000007924 injection Substances 0.000 claims abstract description 11
- 238000010865 video microscopy Methods 0.000 claims abstract description 11
- 238000005485 electric heating Methods 0.000 claims abstract description 6
- 238000005096 rolling process Methods 0.000 claims abstract description 5
- 239000007789 gas Substances 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000001301 oxygen Substances 0.000 claims description 17
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 7
- 230000001954 sterilising Effects 0.000 claims description 5
- 238000010257 thawing Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000004698 Polyethylene (PE) Substances 0.000 claims description 4
- 230000001070 adhesive Effects 0.000 claims description 4
- 239000000853 adhesive Substances 0.000 claims description 4
- 125000004122 cyclic group Chemical group 0.000 claims description 4
- -1 polyethylene Polymers 0.000 claims description 4
- 229920000573 polyethylene Polymers 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 230000000875 corresponding Effects 0.000 claims description 3
- 230000001939 inductive effect Effects 0.000 claims description 3
- 230000001737 promoting Effects 0.000 claims description 3
- 238000004088 simulation Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000001276 controlling effect Effects 0.000 claims 1
- 230000000243 photosynthetic Effects 0.000 claims 1
- 230000004083 survival Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 230000001429 stepping Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a kind of biotechnology cell incubators, including babinet, the inner bottom of the babinet is installed with supporting plate by electric cylinders ontology, and the top center position of supporting plate is rotatably connected to rotating disk by rolling bearing, the top of the supporting plate, which is located on the outside of rotating disk, is inlaid with electric heating tube, and the interior side of babinet is equipped with the videomicroscopy ontology of end face culture vessel, the top rear of the babinet is hinged with case lid, and position is installed with headlamp among the bottom of case lid, the top center position of the case lid is installed with servo motor, the output shaft of the servo motor is fixedly connected with mounting bracket by supporting rod, the top side of the case lid is fixed with the air injection pipe for being plugged with rubber stopper.The inventive structure designs advantages of simple, easy to operate, vdiverse in function, reduces culture difficulty, improves the survival rate of cell culture, safety and stability is applied widely the case where being convenient for observing cell culture, is conducive to popularize.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of biotechnology cell incubator also relates to one
Kind biotechnology cell culture processes.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.No matter for
For entire biotechnology, or the biological cloning technology of one of them, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell.Cell culture technology can by a cell by mass propgation at
For many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself
It is exactly the clone of cell.A large amount of cell or its metabolite are obtained by cell culture.Because biological product is all from cell
It gets, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology.
Cell culture refers to all in vitro cultures, and the growth of cell needs nutrient environment, while also being wanted to extraneous factor
Ask relatively high, existing cell culture is cultivated using the insulating box that can only control temperature mostly, and for other adjustings
Or there is no good management and control for extraneous factor, to influence the survival rate and quality of cell culture, while in cell culture
When also need to using centrifugal device to after defrosting cell liquid carry out centrifugally operated, increase the difficulty of cell culture, and
Existing incubator is not easy to the quality of observation cell culture, is unfavorable for widely popularizing.
Invention content
The purpose of the present invention is to provide a kind of biotechnology cell incubator and its cultural methods, to solve the above-mentioned back of the body
The problem of cell culture difficulty is big, and survival rate is low and is not easy to observe cell culture situation is proposed in scape technology.
To achieve the above object, present invention employs following technical solutions：
The inner bottom of a kind of biotechnology cell incubator, including babinet, the babinet is fixed by electric cylinders ontology
Supporting plate is installed, and the top center position of supporting plate is rotatably connected to rotating disk, the top of the rotating disk by rolling bearing
Activity is placed with culture vessel, and the bottom of supporting plate is installed with the stepping electricity that output shaft is fixedly connected with rotation pan bottom
Machine, the top of the supporting plate, which is located on the outside of rotating disk, is inlaid with electric heating tube, and the interior side of babinet is equipped with end face
The videomicroscopy ontology of culture vessel, the side of the babinet be equipped with the matched observation eyepiece of videomicroscopy ontology,
The inside other side of the babinet is equipped with sensor module, and the top rear of babinet is hinged with case lid, and in the bottom of case lid
Between position be installed with headlamp, the bottom of the case lid is located on the outside of headlamp is inlaid with ultraviolet radiator, and case lid
Top center position is installed with servo motor, and the output shaft of the servo motor is fixedly connected with installation by supporting rod
Frame, and the top of mounting bracket is equipped with the test tube putting hole of adhesive seal washer, the top side of the case lid, which is fixed with, is plugged with rubber
The air injection pipe of rubber plug.
Preferably, four groups of yielding rubber pads, and the positive top of babinet are symmetrically pasted at the bottom corners of the babinet
Both sides and the front end both sides of case lid are equipped with mutually matched hasp.
Preferably, the electric cylinders ontology is at least arranged with four groups, and the surface of electric cylinders ontology is cased with damping spring.
Preferably, the top of the rotating disk is equipped with groove corresponding with culture vessel low side size, and groove is interior
Surface mount has anti-skid rubber cushion.
Preferably, the sensor module includes temperature sensor, gas concentration lwevel sensor and oxygen concentration sensing
Device.
Preferably, the mounting bracket is cyclic annular plate body made of polyethylene, and test tube putting hole is angularly at least provided with eight
Group.
Preferably, the front of the babinet is embedded with the FPGA controller with LED display, and electric cylinders ontology, stepping electricity
Mechanical, electrical heating tube, videomicroscopy ontology, sensor module, headlamp, ultraviolet radiator and servo motor by conducting wire with
FPGA controller is electrically connected.
The present invention also provides a kind of cultural methods of biotechnology cell, include the following steps：
S1, first the cell solution after defrosting is put into centrifuge tube, the test tube then centrifuge tube being inserted at the top of mounting bracket
In putting hole, then by the drive mounting bracket rotation of FPGA controller control servomotor, the cell liquid in centrifuge tube is made to centrifuge 3-5
Minute, and the rotating speed of servo motor is in 2000-4000r/min；
Culture vessel, then is put into support by S2 and then being poured into culture vessel after the cell liquid removal supernatant after centrifugation
It is fixed in rotating disk at the top of plate, it is worked using FPGA controller control point heating tube to adding in babinet after covering end cap
Heat makes the temperature in babinet be maintained at 25 DEG C -45 DEG C；
Temperature, gas concentration lwevel and oxygen concentration in the real-time induction-box body of S3, sensor module, and the number of induction
Control is shown after passing to FPGA controller processing analysis on the LED display on its surface, then from air injection pipe toward babinet
Interior injection oxygen and carbon dioxide gas, make the gas concentration lwevel in babinet be maintained at 20%-30%, and oxygen concentration is maintained at
40%-50%；
S4, when to cell culture by FPGA controller control electric cylinders ontology lifting so that supporting plate is shaken, same to time control
Stepper motor processed drives rotating disk rotation, keeps culture solution uniform with cell distribution to make the cell liquid in culture vessel shake,
And it is heated and to receive extraneous factor uniform；
S5, control headlamp work simulation sunlight may be implemented to be irradiated plant cell the light for promoting plant cell
Cooperation is used, and the work of control ultraviolet radiator can be reduced bacterium and polluted to cell to carrying out sterilizing operation in babinet.
The technique effect and advantage of the present invention：A kind of biotechnology cell incubator proposed by the present invention and its culture side
Method, compared with prior art, have it is following a little：
1, rotation rolling can be carried out to the culture vessel in supporting plate rotary-top disk by electric cylinders body fits stepper motor
It is dynamic, to realize that cell in culture vessel and culture solution are uniformly mixed and receive the uniform of extraneous factor, improve cell culture
Effect；
2, coordinate sensor module can be in order to the temperature in babinet, gas concentration lwevel by heating tube and air injection pipe
Management and control is carried out with oxygen concentration, and headlamp can simulate the photosynthesis that sunlight promotes plant cell；
It 3, can be in order to the cell culture situation progress in culture vessel using videomicroscopy ontology and observation eyepiece
Observation shooting, while ultraviolet radiator may be implemented, to carrying out sterilizing operation in babinet, to reduce bacterium and cause cell culture
It influences；
It 4, can be in centrifuge tube by the test tube putting hole at the top of servo motor at the top of the mounting bracket and mounting bracket of connection
Cell carry out centrifugally operated, reduce the operation difficulty of cell culture, improve the quality of cell culture.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the front section view of the present invention；
Fig. 3 is the circuit module schematic diagram of the present invention；
In figure：1 babinet, 2 electric cylinders ontologies, 3 supporting plates, 4 rotating disks, 5 stepper motors, 6 culture vessels, 7 electric heating tubes, 8 regard
Frequency microscope ontology, 9 observation eyepieces, 10 sensor modules, 11 case lids, 12 headlamps, 13 ultraviolet radiator, 14 servo motors, 15
Mounting bracket, 16 test tube putting holes, 17 air injection pipes, 18 hasps, 19 FPGA controllers.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Herein
Described specific embodiment is only used to explain the present invention, is not intended to limit the present invention.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
The present invention provides a kind of biotechnology cell incubator as shown in Figs. 1-3, including babinet 1, the babinets 1
Inner bottom supporting plate 3 is installed with by electric cylinders ontology 2, and the top center position of supporting plate 3 is rotated by rolling bearing
Be connected with rotating disk 4, the top movable of the rotating disk 4 is placed with culture vessel 6, and the bottom of supporting plate 3 be installed with it is defeated
The stepper motor 5 that shaft is fixedly connected with 4 bottom of rotating disk, the outside that the top of the supporting plate 3 is located at rotating disk 4 are inlaid with electricity
Heating tube 7, and videomicroscopy ontology 8 of the interior side of babinet 1 equipped with end face culture vessel 6, the one of the babinet 1
Side is equipped with is equipped with sensor module with 8 matched observation eyepiece 9 of videomicroscopy ontology, the inside other side of the babinet 1
10, and the top rear of babinet 1 is hinged with case lid 11, and position is installed with headlamp 12, institute among the bottom of case lid 11
The bottom for stating case lid 11 is located at the outside of headlamp 12 and is inlaid with ultraviolet radiator 13, and peace is fixed at the top center position of case lid 11
Equipped with servo motor 14, the output shaft of the servo motor 14 is fixedly connected with mounting bracket 15, and mounting bracket 15 by supporting rod
Top be equipped with the test tube putting hole 16 of adhesive seal washer, the top side of the case lid 11 is fixed with the note for being plugged with rubber stopper
Tracheae 17.
Preferably, four groups of yielding rubber pads are symmetrically pasted at the bottom corners of the babinet 1, and the front top of babinet 1
Both sides and the front end both sides of case lid 11 are held to be equipped with mutually matched hasp 18.
The stability and cushioning ability of babinet 1 can be improved by yielding rubber pad, and hasp 18 can be in order to case lid 11
Closing and opening.
Preferably, the electric cylinders ontology 2 is at least arranged with four groups, and the surface of electric cylinders ontology 2 is cased with damping spring.
By the damping spring of multigroup electric cylinders ontology 2 and its surface can improve supporting plate 3 electric cylinders ontology 2 drive under shake
When stability and cushioning ability.
Preferably, the top of the rotating disk 4 is equipped with corresponding with 6 low side size of culture vessel groove, and groove
Inner surface is pasted with anti-skid rubber cushion.
Groove by being pasted with anti-skid rubber cushion can be fixed in order to the placement of culture vessel 6, while prevent incubator
Ware 6 slides, and improves stability.
It is passed preferably, the sensor module 10 includes temperature sensor, gas concentration lwevel sensor and oxygen concentration
Sensor.
It can in real time be detected in babinet 1 by temperature sensor, gas concentration lwevel sensor and oxygen concentration sensor
Temperature, gas concentration lwevel and oxygen concentration, consequently facilitating to temperature, gas concentration lwevel and oxygen concentration in babinet 1
Carry out management and control.
Preferably, the mounting bracket 15 is cyclic annular plate body made of polyethylene, and test tube putting hole 16 is angularly at least set
There are eight groups.
The test tube putting hole 16 of cyclic annular mounting bracket 15 and multigroup adhesive seal washer made of polyethylene may be implemented
Placement fixation to centrifuge tube and realization centrifugally operated.
Preferably, the front of the babinet 1 is embedded with the FPGA controller 19 with LED display, and electric cylinders ontology 2, step
Stepper motor 5, electric heating tube 7, videomicroscopy ontology 8, sensor module 10, headlamp 12, ultraviolet radiator 13 and servo motor
14 are electrically connected with FPGA controller 19 by conducting wire.
The present invention also provides a kind of cultural methods of biotechnology cell, include the following steps：
S1, first the cell solution after defrosting is put into centrifuge tube, the test tube then centrifuge tube being inserted at the top of mounting bracket
In putting hole, then by the drive mounting bracket rotation of FPGA controller control servomotor, the cell liquid in centrifuge tube is made to centrifuge 3-5
Minute, and the rotating speed of servo motor is in 2000-4000r/min；
Culture vessel, then is put into support by S2 and then being poured into culture vessel after the cell liquid removal supernatant after centrifugation
It is fixed in rotating disk at the top of plate, it is worked using FPGA controller control point heating tube to adding in babinet after covering end cap
Heat makes the temperature in babinet be maintained at 25 DEG C -45 DEG C；
Temperature, gas concentration lwevel and oxygen concentration in the real-time induction-box body of S3, sensor module, and the number of induction
Control is shown after passing to FPGA controller processing analysis on the LED display on its surface, then from air injection pipe toward babinet
Interior injection oxygen and carbon dioxide gas, make the gas concentration lwevel in babinet be maintained at 20%-30%, and oxygen concentration is maintained at
40%-50%；
S4, when to cell culture by FPGA controller control electric cylinders ontology lifting so that supporting plate is shaken, same to time control
Stepper motor processed drives rotating disk rotation, keeps culture solution uniform with cell distribution to make the cell liquid in culture vessel shake,
And it is heated and to receive extraneous factor uniform；
S5, control headlamp work simulation sunlight may be implemented to be irradiated plant cell the light for promoting plant cell
Cooperation is used, and the work of control ultraviolet radiator can be reduced bacterium and polluted to cell to carrying out sterilizing operation in babinet.
In summary：The cell solution after defrosting is put into centrifuge tube first, centrifuge tube is then inserted into mounting bracket top
In the test tube putting hole in portion, mounting bracket rotation is driven by servo motor, so that the cell liquid in centrifuge tube is carried out centrifugally operated, leads to
Rotational wobble can be carried out to the culture vessel in supporting plate rotary-top disk by crossing electric cylinders body fits stepper motor, to realize training
The cell and culture solution supported in vessel are uniformly mixed and receive the uniform of extraneous factor, pass through heating tube and air injection pipe cooperation sensing
Device assembly can be in order to carry out management and control to temperature, gas concentration lwevel and the oxygen concentration in babinet, and headlamp can simulate
Sunlight promotes the photosynthesis of plant cell, can be in order to culture vessel using videomicroscopy ontology and observation eyepiece
Interior cell culture situation carries out observation shooting, while ultraviolet radiator may be implemented to carrying out sterilizing operation in babinet, should
Inventive structure designs advantages of simple, easy to operate, vdiverse in function, reduces culture difficulty, improves the survival rate of cell culture, safety
The case where stablizing, being convenient for observation cell culture, it is applied widely, be conducive to popularize.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (8)
1. a kind of biotechnology cell incubator, including babinet (1), it is characterised in that：The inner bottom of the babinet (1) is logical
It crosses electric cylinders ontology (2) and is installed with supporting plate (3), and the top center position of supporting plate (3) is rotatably connected to by rolling bearing
The top movable of rotating disk (4), the rotating disk (4) is placed with culture vessel (6), and the bottom of supporting plate (3) is installed with
The top of the stepper motor (5) that output shaft is fixedly connected with rotating disk (4) bottom, the supporting plate (3) is located at the outer of rotating disk (4)
Side is inlaid with electric heating tube (7), and the interior side of babinet (1) is equipped with the videomicroscopy sheet of end face culture vessel (6)
The side of body (8), the babinet (1) is equipped with and videomicroscopy ontology (8) matched observation eyepiece (9), the babinet (1)
The inside other side be equipped with sensor module (10), and the top rear of babinet (1) is hinged with case lid (11), and case lid (11)
Position is installed with headlamp (12) among bottom, and the bottom of the case lid (11), which is located on the outside of headlamp (12), to be inlaid with
Ultraviolet radiator (13), and the top center position of case lid (11) is installed with servo motor (14), the servo motor (14)
Output shaft mounting bracket (15) is fixedly connected with by supporting rod, and the top of mounting bracket (15) is equipped with the examination of adhesive seal washer
Pipe putting hole (16), the top side of the case lid (11) are fixed with the air injection pipe (17) for being plugged with rubber stopper.
2. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The bottom of the babinet (1)
Corner of portion is symmetrically pasted with four groups of yielding rubber pads, and the front end both sides of the positive top both sides and case lid (11) of babinet (1)
Equipped with mutually matched hasp (18).
3. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The electric cylinders ontology (2)
Four groups are at least arranged with, and the surface of electric cylinders ontology (2) is cased with damping spring.
4. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The rotating disk (4)
Top is equipped with groove corresponding with culture vessel (6) low side size, and the inner surface of groove is pasted with anti-skid rubber cushion.
5. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The sensor module
(10) include temperature sensor, gas concentration lwevel sensor and oxygen concentration sensor.
6. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The mounting bracket (15) is
Cyclic annular plate body made of polyethylene, and test tube putting hole (16) is angularly at least provided with eight groups.
7. a kind of biotechnology cell incubator according to claim 1, it is characterised in that：The babinet (1) is just
Face is embedded with the FPGA controller (19) with LED display, and electric cylinders ontology (2), stepper motor (5), electric heating tube (7), regards
Frequency microscope ontology (8), sensor module (10), headlamp (12), ultraviolet radiator (13) and servo motor (14) pass through conducting wire
It is electrically connected with FPGA controller (19).
8. a kind of cultural method of biotechnology cell according to claim 1, which is characterized in that including walking as follows
Suddenly：
S1, first the cell solution after defrosting is put into centrifuge tube, then the test tube that centrifuge tube is inserted at the top of mounting bracket is placed
In hole, then by the drive mounting bracket rotation of FPGA controller control servomotor, the cell liquid in centrifuge tube is made to centrifuge 3-5 points
Clock, and the rotating speed of servo motor is in 2000-4000r/min；
Culture vessel, then is put into supporting plate top by S2 and then being poured into culture vessel after the cell liquid removal supernatant after centrifugation
It is fixed in the rotating disk in portion, it is worked to being heated in babinet, is made using FPGA controller control point heating tube after covering end cap
Temperature in babinet is maintained at 25 DEG C -45 DEG C；
Temperature, gas concentration lwevel and oxygen concentration in the real-time induction-box body of S3, sensor module, and the numerical control of induction is passed
It shows on the LED display on its surface after passing FPGA controller processing analysis, is then noted in babinet from air injection pipe
Enter oxygen and carbon dioxide gas, the gas concentration lwevel in babinet is made to be maintained at 20%-30%, oxygen concentration is maintained at
40%-50%；
S4, the lifting of electric cylinders ontology is controlled by FPGA controller when to cell culture supporting plate is made to shake, while controlling step
Stepper motor drives rotating disk rotation, keeps culture solution uniform with cell distribution to make the cell liquid in culture vessel shake, and
It is heated and to receive extraneous factor uniform；
S5, control headlamp work simulation sunlight may be implemented to be irradiated plant cell in the photosynthetic work for promoting plant cell
With the work of control ultraviolet radiator can be reduced bacterium and polluted to cell to carrying out sterilizing operation in babinet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810397180.XA CN108728333A (en) | 2018-04-28 | 2018-04-28 | A kind of biotechnology cell incubator and its cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810397180.XA CN108728333A (en) | 2018-04-28 | 2018-04-28 | A kind of biotechnology cell incubator and its cultural method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108728333A true CN108728333A (en) | 2018-11-02 |
Family
ID=63939434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810397180.XA Pending CN108728333A (en) | 2018-04-28 | 2018-04-28 | A kind of biotechnology cell incubator and its cultural method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108728333A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452819A (en) * | 2019-07-29 | 2019-11-15 | 西安医学院 | A kind of observable hypobaric hypoxia cell incubator |
| CN110724628A (en) * | 2019-11-21 | 2020-01-24 | 姚小珍 | Cell test tube internal environment adjustment mechanism |
| WO2022036807A1 (en) * | 2020-08-21 | 2022-02-24 | 南京大学 | Living cell culture and real-time observation system and method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002064728A2 (en) * | 2001-02-09 | 2002-08-22 | Daniel Attias | Incubator and incubation method monitoring the organism to be incubated |
| CN102093954A (en) * | 2010-12-16 | 2011-06-15 | 汪华 | Cell culture device and cell culture method |
| CN105251075A (en) * | 2015-09-21 | 2016-01-20 | 闫丽 | Blood transfusion type oncotherapy device |
| CN205528839U (en) * | 2016-04-22 | 2016-08-31 | 广东普奥思生物科技有限公司 | Novel cell culture device |
| WO2017007278A1 (en) * | 2015-07-09 | 2017-01-12 | 사회복지법인 삼성생명공익재단 | Automated cell cultivation device, and method for operating cultivation device |
| CN206502829U (en) * | 2017-02-27 | 2017-09-19 | 苏州大学 | Rotary cell incubator |
| CN207227452U (en) * | 2017-09-20 | 2018-04-13 | 新乡医学院三全学院 | A kind of centrifugation cell incubator |
-
2018
- 2018-04-28 CN CN201810397180.XA patent/CN108728333A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002064728A2 (en) * | 2001-02-09 | 2002-08-22 | Daniel Attias | Incubator and incubation method monitoring the organism to be incubated |
| CN102093954A (en) * | 2010-12-16 | 2011-06-15 | 汪华 | Cell culture device and cell culture method |
| WO2017007278A1 (en) * | 2015-07-09 | 2017-01-12 | 사회복지법인 삼성생명공익재단 | Automated cell cultivation device, and method for operating cultivation device |
| CN105251075A (en) * | 2015-09-21 | 2016-01-20 | 闫丽 | Blood transfusion type oncotherapy device |
| CN205528839U (en) * | 2016-04-22 | 2016-08-31 | 广东普奥思生物科技有限公司 | Novel cell culture device |
| CN206502829U (en) * | 2017-02-27 | 2017-09-19 | 苏州大学 | Rotary cell incubator |
| CN207227452U (en) * | 2017-09-20 | 2018-04-13 | 新乡医学院三全学院 | A kind of centrifugation cell incubator |
Non-Patent Citations (2)
| Title |
|---|
| FREDERIQUE TRIAUD ET AL.: "Evaluation of Automated Cell Culture Incubators", 《JALA TUTORIAL》 * |
| 杨军 等: "一种便携式细胞培养箱的研制", 《传感器与微系统》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452819A (en) * | 2019-07-29 | 2019-11-15 | 西安医学院 | A kind of observable hypobaric hypoxia cell incubator |
| CN110724628A (en) * | 2019-11-21 | 2020-01-24 | 姚小珍 | Cell test tube internal environment adjustment mechanism |
| WO2022036807A1 (en) * | 2020-08-21 | 2022-02-24 | 南京大学 | Living cell culture and real-time observation system and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108728333A (en) | A kind of biotechnology cell incubator and its cultural method | |
| CN102199537B (en) | Membrane bioreactor used in microgravity environment and simulated microgravity environment | |
| CN209383811U (en) | A kind of dedicated cell incubator of medical experiment | |
| CN103230623A (en) | Method for in-vitro construction of tissue engineered nerves | |
| CN109439544A (en) | A kind of carbon dioxide cell incubator | |
| CN202730113U (en) | Microalgae high-density culture plant | |
| CN104694390A (en) | Automatic chick embryo incubator for balanced displacement experiment | |
| CN103484356A (en) | Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose | |
| CN207227452U (en) | A kind of centrifugation cell incubator | |
| CN203144384U (en) | Illumination fermentation pot | |
| CN209584228U (en) | A kind of high school student's biofermentation experimental provision | |
| CN111334429A (en) | Modular multifunctional cell workstation | |
| CN208081875U (en) | A kind of separate type evaporator for bio-pharmaceuticals | |
| CN101353624B (en) | Apparatus for observing cell biology behaviors under external DC electric field | |
| CN112899161A (en) | Cell culture device | |
| KR102006427B1 (en) | Apparatus for Cultivating Psychrophilic Microalgae | |
| CN205266557U (en) | Simple and easy plant separation culture apparatus | |
| CN211595675U (en) | Online ultrasonic stripping cell disruption bioreactor | |
| CN209352920U (en) | A kind of photosynthetic cultivation reactor of culture algae | |
| CN205473827U (en) | Cell slide's slide glass support | |
| CN109929762A (en) | A kind of cell culture apparatus | |
| CN106281989A (en) | Laboratory combination type tray solid-state fermentation installation | |
| CN206266577U (en) | A kind of microorganism bed | |
| CN104017768B (en) | Cells in vitro collection method and application, cell in vitro material and application thereof | |
| CN206553525U (en) | A kind of enzyme engineering mushroom prepares special incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181102 |