CN103756874A - Alga culture device with indoor controllable culture conditions - Google Patents

Alga culture device with indoor controllable culture conditions Download PDF

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Publication number
CN103756874A
CN103756874A CN201410025665.8A CN201410025665A CN103756874A CN 103756874 A CN103756874 A CN 103756874A CN 201410025665 A CN201410025665 A CN 201410025665A CN 103756874 A CN103756874 A CN 103756874A
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China
Prior art keywords
ring groove
rotation axis
plug
flow blade
adjustable motor
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Pending
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CN201410025665.8A
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Chinese (zh)
Inventor
李林
朱伟
谢奉君
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Jiangxi Normal University
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Jiangxi Normal University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Catching Or Destruction (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model provides an alga culture apparatus of indoor controllable condition of cultivateing, the bottom of ring channel is equipped with the bottom plate, and the rotation axis is connected to the bearing is vertical, and the top horizontal connection of rotation axis rotates the xarm, and rotation axis connection adjustable motor passes through the connecting wire and connects the speed regulator, the both ends of rotating the xarm respectively vertical connection impeller blade, impeller blade is in the vallecular cavity of ring channel, the bilateral symmetry of ring channel places the light source. The invention has the advantages that: realizes temperature control and light control, and provides hydrodynamic condition tests of different culture conditions. In the process of culturing algae by the experimental device, the blades rotate at a constant speed, so that the problems of unstable and uneven flow rate and the like can be effectively solved.

Description

A kind of algatron of indoor controlled culture condition
Technical field
The present invention relates to micro-algae and cultivate biological technical field, be specifically related to a kind of algatron of indoor controlled culture condition.
Background technology
With regard to the origin cause of formation and the primary condition of breakout of cyanobacteria blooms, carried out large quantity research both at home and abroad, the many environmental factorss such as physilogical characteristics, nutrition salinity and temperature, illumination and other biology of breaking out general and blue-green algae that obtain blue-green alga bloom are closely related.Wherein, very important effect has been played in hydrodynamism, this act in shallow lake more remarkable.By the analysis to 12 Taihu Lake Blue-green Algae Fast Growth Typical Cases of 1986-2007, find that flow condition is one of main induction factor causing Blue-green Algae Fast Growth; Also have some research reports, in lake, flowing of water body may more can affect the growth of blue-green algae than the content of nutrient salt in water itself.Visible significant on the concrete research of blue algae growth impact with regard to current.
Existing indoor culture device, as isothermal vibration incubator, large-scale tank, the devices such as rotor carry out lab simulation culture experiment, the device using forms loaded down with trivial details, culture environment condition is wayward, and water volume flow rate is wayward etc., is not well positioned to meet the indoor cultivation test requirements document of single analysis water power factor pair blue-green algae physiology, biochemistry.
According to frustule growth characteristics, in its process of growth, higher to illumination, temperature, dissolved oxygen, the environmental requirement such as aseptic, so culture apparatus needs the envrionment conditions of accurate Growth of Microcystis in Water.There is the problems such as design is loaded down with trivial details, test operation is complicated, and repeatable poor, test fee material is required great effort, and energy consumption is larger, test poor operability in traditional algatron.
Summary of the invention
The object of the present invention is to provide a kind of algatron of indoor controlled culture condition, can under hydrodynamic(al) water condition, understand the impact of different in flow rate on algal grown, not only manufacture craft is simple, starting material cost is low, energy saving economy, and easy to operate, practical.
The present invention is such, it comprises speed regulator, wire, adjustable motor, ring groove, vallecular cavity, rotate transverse arm, rotation axis, lamp, plug-flow blade, bearing, base plate, it is characterized in that: the bottom of ring groove is provided with base plate, the vertical connection of rotating axle of bearing, the top level connection joint of rotation axis rotates transverse arm, rotate transverse arm and vertically connect plug-flow blade, rotation axis is connected with adjustable motor, adjustable motor connects speed regulator by wire, the two ends of described rotation transverse arm vertically connect respectively plug-flow blade, plug-flow blade is in the vallecular cavity of ring groove, the zygomorphy of described ring groove is placed illuminator.
Described plug-flow blade and ring groove are synthetic glass material.
Described plug-flow blade and the inside and outside wall of ring groove gap 0.5mm.
Blade, under the driving of adjustable motor, promotes the mobile current that produce of trough inner water body, by regulating the flow velocity of water body in motor speed control flume, guarantees the stability of current.Plug-flow blade and ring groove material used is synthetic glass, can ultraviolet light sterilization before cultivation.
Adjustable motor is crossed rotating shaft with blade pass and is connected, thereby promotes blade movement.Adjustable motor, by being connected with controller, is controlled its speed, can make blade movement realize stepless time adjustment.The symmetrical lamp of placing of ring groove both sides external, as light source, provides illumination, can control light intensity, for realizing different light intensity test.
The invention has the advantages that:
1. experimental installation, in the process of algae culture, has been realized temperature control, control light, and the hydrodynamic condition test of different culture condition is provided.
2. experimental installation is in the process of algae culture, and blade at the uniform velocity high-efficient operation has solved unstable, inhomogeneous etc. the problem of flow velocity effectively.
3. experimental installation, in the process of algae culture, has been realized the impact of avoiding bacterium simultaneously, strictly under aseptic condition, cultivates.
4. the flow rate of water flow radial distribution producing in ring groove is even, is convenient to understand the impact of water volume flow rate on algal grown.
5. experimental installation is easy and simple to handle, and the equipment such as motor, lamp are convenient to regulation and control.
6. experimental installation is simple in structure, and small portable is practical, and repeatability is strong.
7. experimental installation starting material wide material sources, technique is simple, easy to process, saves human and material resources and financial resources.
8. experimental installation motor and blade all can be dismantled flexibly, are convenient to cleaning, sterilizing.
Accompanying drawing explanation
Accompanying drawing 1 is structural representation of the present invention.
In figure 1 is speed regulator, the 2nd, and wire, the 3rd, adjustable motor, the 4th, ring groove, the 5th, vallecular cavity, the 6th, rotate transverse arm, the 7th, rotation axis, the 8th, illuminator, the 9th, plug-flow blade, the 10th, bearing, the 11st, base plate.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described.
Contrast accompanying drawing 1, the present invention is such, the bottom of ring groove 4 is provided with base plate 11, the upper surface of base plate 11 is by the vertical connection of rotating axle 7 of bearing 10, and the top level connection joint of rotation axis 7 rotates transverse arm 6, rotates transverse arm 6 and connects adjustable motor 3, adjustable motor 3 connects speed regulator 1 by wire 2, the two ends of described rotation transverse arm 6 vertically connect respectively plug-flow blade 9, and plug-flow blade 9 is in the vallecular cavity 5 of ring groove 4, and illuminator 8 is placed respectively in the both sides of described ring groove 4.Plug-flow blade and ring groove are synthetic glass material.Rotating shaft and adjustable motor are settled in ring groove center, the inner plug-flow blade of placing of ring groove.Adjustable motor is crossed rotating shaft with plug-flow blade pass and is connected, the running of motor driven vane.Motor, blade are all detachable, adjust flexibly.
Motor rated power is 60w, and it is connected with speed regulator, and power supply is used 220v alternating-current.By speed regulator, regulate adjustable motor rotating speed, thereby drive plug-flow blade movement, promote trough inner water body and flow, and water body flow in control flume, stability and the continuity of assurance current.The inside and outside wall of plug-flow blade and ring groove gap 0.5mm, avoids frictional influence also can evenly promote water body simultaneously.Promoting current is to make the state of algae in suspending, and make it carry out photosynthesis, and nutrient distribution is even.Ring groove maximum is held substratum 1800ml, and working volume is 1500ml.Water intaking sampling point is set in groove, is positioned at 75mm eminence.Lamp is placed as light source in ring slot outside both sides, conveniently can control light intensity, and can carry out illumination condition test.
Whole device technique is simple, easy to operate, has the fine water power culture experiment effect that realizes simultaneously.

Claims (3)

1. the algatron of an indoor controlled culture condition, it comprises speed regulator, wire, adjustable motor, ring groove, vallecular cavity, rotate transverse arm, rotation axis, lamp, plug-flow blade, bearing, base plate, it is characterized in that: the bottom of ring groove is provided with base plate, the vertical connection of rotating axle of bearing, the top level connection joint of rotation axis rotates transverse arm, rotation axis connects adjustable motor, adjustable motor connects speed regulator by wire, the two ends of described rotation transverse arm vertically connect respectively plug-flow blade, plug-flow blade is in the vallecular cavity of ring groove, the zygomorphy of described ring groove is placed illuminator.
2. the algatron of a kind of indoor controlled culture condition according to claim 1, is characterized in that: described plug-flow blade and ring groove are synthetic glass material.
3. the algatron of a kind of indoor controlled culture condition according to claim 1, is characterized in that: described plug-flow blade and ring groove inwall gap 0.5mm.
CN201410025665.8A 2014-01-21 2014-01-21 Alga culture device with indoor controllable culture conditions Pending CN103756874A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106066379A (en) * 2016-06-21 2016-11-02 常州市环境科学研究院 Bloom blue algae is by wind disturbance Vertical Migration analog round the clock
CN108243933A (en) * 2017-12-26 2018-07-06 华北电力大学 A kind of experimental provision simulated hydraulics and influenced on plankton community
JP2020018191A (en) * 2018-07-31 2020-02-06 岡部株式会社 Filament agitation culture device for seaweed
CN113925004A (en) * 2021-11-29 2022-01-14 重庆交通大学 Fish egg hatching system based on river course live simulation
CN113951186A (en) * 2021-11-29 2022-01-21 重庆交通大学 Test method for researching influence of turbulence on development process of roe embryo body
CN113951185A (en) * 2021-11-29 2022-01-21 重庆交通大学 Drifting fish egg hatching method
CN113951187A (en) * 2021-11-29 2022-01-21 重庆交通大学 Drifting fish egg embryo body development test device

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN201517107U (en) * 2009-08-31 2010-06-30 环境保护部南京环境科学研究所 Alga experiment analog device for controlling temperature, light and flow rate

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Publication number Priority date Publication date Assignee Title
CN201517107U (en) * 2009-08-31 2010-06-30 环境保护部南京环境科学研究所 Alga experiment analog device for controlling temperature, light and flow rate

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吴晓辉和李其军: "水动力条件对藻类影响的研究进展", 《生态环境学报》, vol. 19, no. 7, 31 December 2010 (2010-12-31) *
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106066379A (en) * 2016-06-21 2016-11-02 常州市环境科学研究院 Bloom blue algae is by wind disturbance Vertical Migration analog round the clock
CN108243933A (en) * 2017-12-26 2018-07-06 华北电力大学 A kind of experimental provision simulated hydraulics and influenced on plankton community
JP2020018191A (en) * 2018-07-31 2020-02-06 岡部株式会社 Filament agitation culture device for seaweed
JP7091182B2 (en) 2018-07-31 2022-06-27 岡部株式会社 Stirring and culturing equipment for filamentous bodies of seaweed
CN113925004A (en) * 2021-11-29 2022-01-14 重庆交通大学 Fish egg hatching system based on river course live simulation
CN113951186A (en) * 2021-11-29 2022-01-21 重庆交通大学 Test method for researching influence of turbulence on development process of roe embryo body
CN113951185A (en) * 2021-11-29 2022-01-21 重庆交通大学 Drifting fish egg hatching method
CN113951187A (en) * 2021-11-29 2022-01-21 重庆交通大学 Drifting fish egg embryo body development test device
CN116138192A (en) * 2021-11-29 2023-05-23 重庆交通大学 A push pedal structure for fish egg hatching
CN116138192B (en) * 2021-11-29 2024-04-19 重庆交通大学 A push pedal structure for fish egg hatching

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Application publication date: 20140430