CN109749929A - A kind of brain slice in vitro rewarming incubating device of real-time monitoring state - Google Patents
A kind of brain slice in vitro rewarming incubating device of real-time monitoring state Download PDFInfo
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- CN109749929A CN109749929A CN201910132954.0A CN201910132954A CN109749929A CN 109749929 A CN109749929 A CN 109749929A CN 201910132954 A CN201910132954 A CN 201910132954A CN 109749929 A CN109749929 A CN 109749929A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a kind of brain slice in vitro rewarming incubating device of real-time monitoring state, including shading cover, fixed plate, bath, panel, heating plate, brain piece hold slip through the net, oxygen supply system, monitoring system and control centre；Wherein, brain piece holds to slip through the net and be arranged in fixed plate, and the top of bath is arranged in fixed plate；Monitoring system includes osmotic pressure detection probe, temperature measuring probe, oxygen concentration monitoring probe, pH value monitoring probe and panel, and monitoring system connects control centre；The bottom in bath is arranged in oxygen supply system, and oxygen supply system connects source of oxygen；The medial surface of bath is arranged in heating plate, is provided with outlet behind bath；It realizes temperature to be precisely controlled, multiple brain piece rewarmings can be made to restore in body state in real time, brain piece keep-alive rate is improved, effectively increase brain slice in vitro rewarming and be incubated for success rate, had laid a good foundation for the record experiment of subsequent neuron.
Description
Technical field
The invention belongs to experimental provision fields, and in particular to a kind of brain slice in vitro rewarming incubation dress of real-time monitoring state
It sets.
Background technique
Patch clamp technique is the important research technology in Neuroscience Research field.The technology can record the electricity of single neuron
The electrical informations such as pressure, electric current, cell film resistance are widely used in Neuroscience Research, neurologic agent screening, neurological disease mould
Type detection, neuron state identification etc..Although the technology is one important technology of neuroscience field, factors system
About the technology universal and promote.Wherein, how to allow in vitro low temperature brain piece rewarming as far as possible be incubated for the shape similar in body
State is maximum limiting factor.In order to obtain the brain slice in vitro for record, experimental animal need to take out rapidly brain under narcosis
Tissue.Under stress situation, animal brain's faster metabolism reduces extraneous damage tolerance.In order to reduce damage as far as possible
Wound, brain tissue need to be bathed in the brain piece being uniformly cut into the artificial cerebrospinal fluid of 0 degrees centigrade with a thickness of 300 microns, at this time
Neuron is in " shock " state.In order to restore neuron to the state similar in body, the brain piece cut need to be transferred to 26
~28 degrees Celsius of 95%O2/ 5%CO2Rewarming is incubated for 1 hour in the artificial cerebrospinal fluid of sustainable supply.Whether rewarming incubation succeeds, directly
It connects and is related to the later period and can go on smoothly record.Influencing the principal element that brain piece rewarming is incubated for is following three points: temperature, oxygen are dense
Degree, pH value, osmotic pressure.
Existing rewarming incubating device is each laboratory self-control, is typically configured to water-bath (for heating up), beaker
(holding artificial cerebrospinal fluid), floating slips through the net and (holds brain piece) and oxygen hose (providing oxygen)；There are many deficiencies for existing apparatus
Place:
1, can not real-time monitoring artificial cerebrospinal fluid temperature.Water-bath can only monitor the temperature of water, and really influence rewarming
Be incubated for effect is artificial cerebrospinal fluid；
2, it uniformly can not supply oxygen and monitor oxygen saturation.Farther away brain piece, which is exported, from oxygen hose is usually unable to get enough oxygen
Gas often results in irreversible damage since neuron is extremely sensitive to anoxic.Existing apparatus can only estimate by rule of thumb, lack
Unified measurement index；
3, it is unable to monitor the variation of pH value.Preferably, meta-acid or meta-alkali will affect activity neuron within physiological range
Neuronal activity；
4, it is unable to monitor the osmotic pressure of artificial cerebrospinal fluid.Excessively high osmotic pressure will cause cell dehydration shrinkage, lower infiltration
Pressure causes cell water suction to burst thoroughly.
5, lack and be protected from light protective device.Some brain piece internal representations have fluorescence, and being exposed to will cause fluorescence under natural light and quench
It goes out, is unable to reach expected object of experiment；Secondly artificial cerebrospinal fluid exposes in air, vulnerable to pollution；
6, it is limited to hold brain piece position.Since brain piece can not stack during incubation and (stack the easy anoxic in place), Mei Gekong
One can be only placed in hole.It is unable to satisfy high-volume experiment demand.
Summary of the invention
The purpose of the present invention is to provide a kind of brain slice in vitro rewarming incubating device of real-time monitoring state and operating method,
To solve the restraining factors during brain slice in vitro rewarming is incubated for.
To reach realization above-mentioned purpose, the present invention adopts the following technical scheme: a kind of brain slice in vitro of real-time monitoring state
Rewarming incubating device, including shading cover, fixed plate, bath, panel, heating plate, brain piece hold and slip through the net, oxygen supply system, supervise
Examining system and control centre；Wherein, brain piece holds to slip through the net and be arranged in fixed plate, and the top of bath is arranged in fixed plate；Prison
Examining system includes osmotic pressure detection probe, temperature measuring probe, oxygen concentration monitoring probe, pH value monitoring probe and panel,
Monitoring system connects control centre, is provided with temperature display, osmotic pressure display screen, oxygen concentration display screen, pH value on panel
Display screen, warning lamp, temperature regulating key and switch；The bottom in bath, oxygen supply system is arranged in oxygen supply system
System connection source of oxygen；The medial surface of bath is arranged in heating plate, is provided with outlet on the outside of bath.
Oxygen supply system includes that unidirectional electric control valve, oxygen supply pipeline and oxygen froth head, oxygen supply pipeline
It is uniformly arranged including a main pipe rail and several bye-passes, bye-pass in bath bottom, oxygen is uniformly arranged on bye-pass and is frothed
Head；Unidirectional electric control valve is arranged at the oxygen intake on main line, and unidirectional electric control valve connects control centre.
Warning lamp includes heating lamp, thermostatical pilot lamp, oxygen warning lamp, pH value warning lamp and osmotic pressure instruction
Lamp, warning lamp connect control centre；It connects and is provided with overload protective device on the way circuit of outlet.
It is equal that brain piece holds the quantity slipped through the net and the oxygen quantity of head that froths, and brain piece holds to slip through the net and froth head positioned at oxygen
Surface.
The bottom of bath is provided with oxygen supply pipeline card slot, and oxygen supply pipeline is arranged in the oxygen supply pipeline card
In slot.
The total length of fixed plate is greater than the distance of the front and rear wall of bath, and the two sides of one end of fixed plate offer gap, Gu
Fixed board is uniformly provided with along its length holds the hole slipped through the net for installing brain piece.
Bath is cube groove, and U-type groove is opened up on bath wall, is equipped with fixed plate rest stand, U-type groove bottom surface inside bath
Height be higher than fixed plate rest stand top, one end of fixed plate is shelved on fixed plate rest stand, and handling section is caught in U-type groove,
Handheld terminal is additionally provided with anti-drop structure.
Fixed plate is bent structure, and the bent structure includes handling section, linkage section and incubation section；It is incubated for Duan Shangjun
Even setting brain piece, which is held, slips through the net, and handling section is parallel with section is incubated for.
Shading cover is lighttight black lid, and the bottom surface of shading cover is additionally provided with and U-type groove size, shape and quantity
Corresponding anti-dazzling screen.
Insulating layer is provided on the outside of bath.
Compared with prior art, the present invention at least has the advantages that the present invention using heating plate directly to artificial
Cerebrospinal fluid carries out plus mild temperature adjustment monitoring, and thermometric is more acurrate, temperature adjustment is more convenient；Temperature is realized to be precisely controlled, it can be in real time
Restore multiple brain piece rewarmings in body state, improve brain piece keep-alive rate, further increase experiment detection efficiency, save the time and
Brain piece resource, and testing result is also more acurrate；Be firstly introduced oxygen concentration, pH value and osmotic pressure real-time monitoring application so that
Brain piece rewarming is incubated for precision, standardizes and unitize, no longer the personal experience of height dependence experimenter；The present invention is from experiment
Demand is set out, and deft design is innovated for multinomial restraining factors, is effectively increased brain slice in vitro rewarming and is incubated for success rate,
It haves laid a good foundation for the record experiment of subsequent neuron.
Further, it is laid with oxygen pipeline in bath bottom, corresponding each brain piece holds the aerobic air-blowing bubble in lower section of slipping through the net
Head is supplied oxygen, and guarantees oxygen supply without dead angle.
Further, the dismounting of brain piece apparatus for placing flexibly, can carry out adding assembly and disassembly according to experiment, can meet all kinds of
Type tests demand.
Further, it is equipped with shading cover at the top of bath of the present invention, brain piece is effectively ensured not by external light influence, simultaneously to the greatest extent
Potentially reduce the pollution of artificial cerebrospinal fluid.
Further, oxygen pipeline passes through card slot and is fixed among bath, cleaning and replacement easy to disassemble.
Detailed description of the invention
Fig. 1 is positive structure diagram of the invention；
Fig. 2 is overlooking structure diagram of the invention；
Fig. 3 is right side structural representation of the invention；
Fig. 4 is functional mode figure of the invention；
Fig. 5 is oxygen supply system positive structure diagram of the invention；
Fig. 6 is oxygen supply system overlooking structure diagram of the invention；
Fig. 7 is oxygen supply system right side structural representation of the invention；
Fig. 8 is brain piece apparatus for placing positive structure diagram of the invention；
Fig. 9 is brain piece apparatus for placing overlooking structure diagram of the invention；
Figure 10 is brain piece apparatus for placing right side structural representation of the invention.
In attached drawing, 1- shading cover, 2- fixed plate, 3- bath, 4- panel, 5- temperature regulating key, 6- thermostatical pilot lamp, 7-
Switch, 8- overload protective device, 9- oxygen interface, 10- outlet, 11- fixed plate rest stand, 12- heating plate, 13- brain piece are contained
It puts and slips through the net, 14- oxygen supply pipeline, 15- oxygen froths head, 17- temperature monitoring probe, 18- oxygen concentration monitoring probe, 19-
PH value monitoring probe, the unidirectional electric control valve of 20-, 21- oxygen supply pipeline card slot,
Specific embodiment
Specific embodiments of the present invention are given below, it should be noted that the invention is not limited to implement in detail below
Example, all equivalent transformations made on the basis of the technical solutions of the present application each fall within protection scope of the present invention.
In compliance with the above technical solution, referring to figs. 1 to Figure 10, the brain slice in vitro rewarming incubating device of real-time monitoring state is specific
Embodiment and operating method improve brain slice in vitro rewarming and are incubated for success rate.
As shown in FIG. 1 to 3, the brain slice in vitro rewarming incubating device of a kind of real-time monitoring state, including it is shading cover 1, solid
Fixed board 2, bath 3, panel 4, heating plate 12, brain piece hold slip through the net 13, oxygen supply system, monitoring system and control centre；
Wherein, brain piece, which is held, slips through the net 13 settings in fixed plate 2, and the top of bath 3 is arranged in fixed plate 2；The connection control of monitoring system
Center, monitoring system include osmotic pressure detection probe 16, temperature measuring probe 17, oxygen concentration monitoring probe 18, pH value monitoring
Probe 19 and panel 4 is provided with temperature display, osmotic pressure display screen, oxygen concentration display screen, pH value on panel 4 and shows
Screen, warning lamp, temperature regulating key 5 and switch 7；The bottom in bath, oxygen supply system is arranged in oxygen supply system
Connect source of oxygen；The medial surface of bath 3 is arranged in heating plate 12, and the outside of bath 3 is provided with outlet 10.
As shown in Fig. 2, Fig. 5 and Fig. 7, oxygen supply system include unidirectional electric control valve 20, oxygen supply pipeline 14 and
Oxygen froths first 15, and oxygen supply pipeline 14 includes a main pipe rail and several bye-passes, and bye-pass is uniformly set in 3 bottom of bath
It sets, oxygen is uniformly arranged on bye-pass and froths first 15；Unidirectional electric control valve 20 is arranged at the oxygen intake on main line, unidirectionally
Electric control valve 20 connects control centre；The bottom of bath 3 is provided with oxygen supply pipeline card slot 21, and oxygen supply pipeline 14 is arranged
In the oxygen supply pipeline card slot 21.
Brain piece hold slip through the net 13 quantity and oxygen froth first 15 quantity it is equal, brain piece holds to slip through the net 13 to be blown positioned at oxygen
Steep the surface of head 15.
As shown in figure 4, warning lamp include heating lamp, thermostatical pilot lamp 6, oxygen warning lamp, pH value warning lamp and
Osmotic pressure indicator light, warning lamp connect control centre；It connects and is provided with overload protective device 8 on the way circuit of outlet 10.
As shown in Fig. 8~Figure 10, the total length of fixed plate 2 is greater than the distance of the front and rear wall of bath 3, one end of fixed plate 2
Two sides offer gap, fixed plate 2 be uniformly provided with along its length for install brain piece hold slip through the net 13 hole.
Bath 3 is cube groove, and U-type groove 22 is opened up on bath wall, and fixed plate rest stand 11 is equipped with inside bath 3, U-shaped
The height of 22 bottom surface of slot is higher than 11 top of fixed plate rest stand, and one end of fixed plate 2 is shelved on fixed plate rest stand 11, holds
Section is caught in U-type groove 22, and handheld terminal is additionally provided with anti-drop structure.
Fixed plate 2 is bent structure, and the bent structure includes handling section, linkage section and incubation section；It is incubated in section
It is uniformly arranged brain piece and holds and slip through the net 13, handling section is parallel with section is incubated for.
Shading cover 1 is lighttight black lid, and the bottom surface of shading cover 1 is additionally provided with and U-type groove size, shape and number
Measure corresponding anti-dazzling screen.
The outside of bath 3 is provided with insulating layer.
A kind of brain slice in vitro rewarming incubating device of real-time monitoring state of the present invention, including monitor control system, display surface
Plate, oxygen supply system, brain piece apparatus for placing, bath 3, shading cover 1；
As shown in Figure 1 and Figure 4, monitoring system include temperature monitoring system, oxygen concentration monitoring system, pH value monitoring system and
Permeate pressure monitoring system；Monitoring system is all connected with control centre, and bath 3 and power supply wiring way circuit are equipped with overload protective device 8,
Overload protective device 8 is for preventing circuit overloads from damaging electronic component.
Temperature monitoring system includes temperature monitoring probe 17, temperature display, thermostatical pilot lamp 6, when energized, temperature
Spend monitoring probe 17 by the temperature feedback of liquid in the bath 3 measured on a display screen.User is as needed, passes through temperature
Regulating key 5 sets desired temperature.If temperature is lower than preset temperature, heating lamp is lighted, and heating plate 12 is started to work, to bath
Liquid in slot 3 heats up；If temperature reaches preset temperature, thermostatical pilot lamp is lighted, and heating plate 12 stops working, stopping pair
Liquid heating.
It includes oxygen concentration monitoring probe 18, oxygen concentration display screen, oxygen warning lamp that oxygen concentration, which monitors system,；When connecing
When energization source, oxygen concentration monitoring probe 18 detects the oxygen concentration of bath tank liquid automatically, and Real-time Feedback is in oxygen concentration
On display screen；If oxygen concentration is not within physiological range, the physiological range of oxygen concentration is 80~100mmHg, and oxygen is alert
Show that lamp lights；When lower than 80mmHg, 20 automatic opening of unidirectional electric control valve is supplied oxygen in oxygen supply system；Oxygen concentration is super
100mmHg is crossed, unidirectional electric control valve 20 is closed, oxygen feeding stop.
It includes pH value monitoring probe 19, pH value display screen and pH value warning lamp that pH value, which monitors system,；When energized, p
The pH value of the detection bath tank liquid automatically of H value monitoring probe 19, and Real-time Feedback is on pH value display screen, if liquid pH is not in life
It manages in range, the physiological range of pH value is 7.35~7.45, and pH value warning lamp lights, and experimenter is prompted to be adjusted.
Permeating pressure monitoring system includes osmotic pressure monitoring probe 16, osmotic pressure display screen and osmotic pressure warning lamp；Work as connection
When power supply, osmotic pressure monitoring probe 16 detects the osmotic pressure of bath tank liquid automatically, and Real-time Feedback is on osmotic pressure display screen；
If Liquid Penetrant pressure, not in physiological range, the physiological range of osmotic pressure is 290mOsm/L~300mOsm/L, osmotic pressure warning
Lamp lights, and experimenter is prompted to be adjusted.
Display panel includes temperature, oxygen concentration, four display areas pH and osmotic pressure；Temperature display region is aobvious equipped with temperature
Predetermined temperature, temperature is arranged by temperature regulating key 5 in display screen, temperature regulating key 5, heating lamp and thermostatical pilot lamp 6
Spend the temperature that display screen shows artificial cerebrospinal fluid in current state bath；When temperature is lower than preset temperature in bath 3, heating refers to
Show that lamp lights, heats up to the liquid in bath 3；When reaching preset temperature, thermostatical pilot lamp 6 is lighted, and stops heating.
Oxygen concentration display area is equipped with oxygen concentration display screen and oxygen warning lamp, when oxygen concentration is above or below 80
When~100m mHg, oxygen warning lamp is lighted；If being lower than 80mmHg, oxygen supply system supplies oxygen automatically；If oxygen concentration is more than
100m mHg, oxygen supply system oxygen feeding stop；PH value display area is equipped with pH value display screen and pH value warning lamp, when pH value is inclined
When from 7.35~7.45 normal range (NR), pH value warning lamp is lighted；Osmotic pressure display area is equipped with osmotic pressure display screen and osmotic pressure
Warning lamp, when osmotic pressure deviates 290~300mOsm/L normal range (NR), osmotic pressure warning lamp is lighted.
Oxygen supply system includes that oxygen interface 9, unidirectional electric control valve 20, oxygen supply pipeline 14 and oxygen froth head
15；The external 95%O of oxygen interface 92/ 5%CO2Gaseous mixture；Unidirectional electric control valve 20 can be such that oxygen flows into from 9 direction of oxygen interface
Oxygen froths first 15, and prevents the artificial cerebrospinal fluid in bath from inversely passing through oxygen supply pipeline 14 and flow out from oxygen interface 9；Always
Four branches are set up on condominium road separately, and every branch road is impartial to froth first 15 there are four oxygen away from setting, oxygen froth first 15 just on
Side, which respectively corresponds to be provided with brain piece and hold, slips through the net 13 so that each brain piece hold the brain piece slipped through the net in 13 can obtain it is sufficient and
The suitable oxygen supply of concentration.
Brain piece apparatus for placing includes fixed plate 2 and brain piece holds and slips through the net 13；2 one end of fixed plate is placed in fixed plate rest stand
11, one section is placed in bath 3, and default recess, the design of " Z " font fixed plate slip through the net 13 deeper into bath so that brain piece is held
Slot reduces the dosage of artificial cerebrospinal fluid, more saving reagent.It is reserved in fixed plate by four circular holes, holds and slip through the net for brain piece
13 set-down locations.Brain piece holds 13 convenience of slipping through the net and slips through the net for circle, and brain piece is incubated in this rewarming, is facilitated in internal liquid and bath
Liquid can exchange in time.
Bath 3 is cube groove, is equipped with fixed plate rest stand 11, heating plate 12 and oxygen supply pipe inside bath 3
An outpost of the tax office's slot 21, bath wall are equipped with U-type groove 22, outlet 7 and oxygen interface 9；Fixed plate rest stand 11 is for placing brain
Piece holds one end of fixed plate 2, and U-type groove 22 is used for 2 other end of fixed plate that brain piece is held, and heating plate 12 is used for people in bath
The heating of work cerebrospinal fluid；Oxygen supply pipeline card slot 21 fixes oxygen supply pipeline 14, prevents its shaking, facilitates oxygen supply pipeline
Installing and dismounting cleaning and replacement.
Shading cover 1 is black rectangle lid, opaque, convenient for being incubated for the protection of brain piece in vitro rewarming.
The working process of the invention is as follows:
The brain slice in vitro rewarming incubating device of real-time monitoring state need to carry out instrument testing before carrying out rewarming incubation；It is first
It need to first be packed into configured artificial cerebrospinal fluid in bath 3, power supply line is inserted into power cord jack 10 and power on, utilize modeling
Expect hose connection oxygen interface 10 and oxygen cylinder；Shading cover 1 is covered, turns on the power switch 7；Brain slice in vitro rewarming is incubated for dress at this time
The monitoring probe set will monitor temperature, four oxygen concentration, pH value and osmotic pressure core index of liquid automatically.
Temperature monitoring system include temperature display, temperature control circuit, heating plate 12, heating lamp, thermostatical pilot lamp with
And temperature monitoring probe 17；After powering on, the temperature of artificial cerebrospinal fluid in 17 automatic sensing bath 3 of temperature monitoring probe, and
On temperature display, experimenter is incubated for feedback as desired by 5 set temperature of temperature regulating key, brain slice in vitro rewarming
Preference temperature between 26~28 DEG C.If fluid temperature is lower than set temperature, heating lamp is lighted, and heating plate 12 works,
It heats up to artificial cerebrospinal fluid.After temperature monitoring probe 17 monitors that fluid temperature reaches set temperature, heating lamp
Extinguish, thermostatical pilot lamp 6 lights, and heating plate 12 stops working.
Oxygen monitoring system includes oxygen concentration display area, control circuit, oxygen concentration monitoring probe 18 and unidirectional
Electric control valve 20 is constituted；In 18 real-time monitoring bath of oxygen concentration monitoring probe the oxygen concentration of artificial cerebrospinal fluid and feed back in oxygen
On gas display screen, oxygen concentration is measured with partial pressure of oxygen, and the partial pressure of oxygen for being suitable for neuronal survival is 80~100mmHg.Oxygen concentration
When not in the normal range, oxygen warning lamp is lighted.After oxygen concentration is more than the upper limit, unidirectional electric control valve 20 is closed, and is stopped
Oxygen supply, after oxygen concentration is lower than lower limit, unidirectional electric control valve 20 is opened greatly, increases oxygen supply；It is incubated for neuron living, can be gradually used up
The oxygen being dissolved in bath foam is more than to stop in time after physiological range, as oxygen consumes, oxygen concentration meeting in artificial spinal fluid
It lowers, is lower than lower limit when dissolving oxygen concentration in a liquid, then needs to be replenished in time.
It includes pH value monitoring probe 19 and pH value display screen, 19 real-time monitoring bath of pH value monitoring probe that pH value, which monitors system,
The pH of interior artificial cerebrospinal fluid changes and result is fed back to pH value display screen, and pH value normal range (NR) is 7.35~7.45, when exceeding
After this range, pH value warning lamp is lighted, and experimenter is prompted to adjust in time.
Oxygen supply system includes that oxygen interface 9, unidirectional electric control valve 20, oxygen supply pipeline 14 and oxygen froth head
15.The external 95%O of oxygen interface 92/ 5%CO2Gaseous mixture；Oxygen supply pipeline is divided into a main pipe rail and four bye-passes.Branch
Pipeline is equidistantly bonded on main pipe rail, and is connected to main pipe rail, oxygen supply pipeline be fixed on oxygen supply pipeline card slot 21 it
On.Every bye-pass is equidistantly set there are four outlet, can be placed oxygen and be frothed first 15.
Brain piece apparatus for placing includes fixed plate 2 and brain piece holds and slips through the net 13；Brain piece is held first slip through the net 13 be placed in it is solid
In the circle cavity reserved on fixed board 2, each fixed plate, which is arranged four brain pieces and holds, slips through the net 13, and after installing, fixed plate is put
It is placed among bath, one end is held on fixed plate rest stand 11, and the other end is held in U-type groove 22, and fixed plate 2 and brain piece are contained
Putting to slip through the net ensures to stablize after the completion of 13 placements, and brain piece holds to slip through the net and 13 be completely submerged among artificial cerebrospinal fluid, the brain piece cut
It can be placed in brain piece and hold to slip through the net and carry out rewarming incubation on 13.
As the preferred embodiment of the invention, when use brain piece need to be held slip through the net 13 should froth with oxygen it is first 15 corresponding,
Guarantee that brain piece can obtain enough oxygen supplies, shading cover 1 should be covered when having artificial cerebrospinal fluid and brain piece in bath, as far as possible
Reduce artificial cerebrospinal fluid volatilization and the damage of brain piece.
The operating method of the brain slice in vitro rewarming incubating device of real-time monitoring state of the present invention, comprising the following steps:
1, prepared artificial cerebrospinal fluid is poured into bath 3, and brain piece apparatus for placing is fixed in bath 3；
2, oxygen is connected, 7 is turned on the power switch, covers shading cover 1, device detects the temperature of artificial cerebrospinal fluid, oxygen automatically
Concentration, pH value and osmotic pressure；
3, set temperature is 28 DEG C, and heating plate 12 automatically heats artificial cerebrospinal fluid；
4, after temperature, oxygen concentration, pH value and osmotic pressure reach parameter preset, it is sliced；
5, it the brain piece cut is put into brain piece holds to slip through the net and carry out rewarming incubation in 13；
6, rewarming can record brain piece after being incubated for one hour, remaining brain piece continues constant-temperature incubation in a device.
Claims (10)
1. a kind of brain slice in vitro rewarming incubating device of real-time monitoring state, which is characterized in that including shading cover (1), fixed plate
(2), bath (3), panel (4), heating plate (12), brain piece, which are held, slips through the net (13), oxygen supply system, monitors system and control
Center；Wherein, brain piece, which is held, slips through the net (13) setting on fixed plate (2), and fixed plate (2) is arranged on the top of bath (3)；Monitoring
System connects control centre, and monitoring system includes osmotic pressure detection probe (16), temperature measuring probe (17), oxygen concentration monitoring
Pop one's head in (18), pH value monitoring probe (19) and panel (4), be provided on panel (4) temperature display, osmotic pressure display screen,
Oxygen concentration display screen, pH value display screen, warning lamp, temperature regulating key (5) and switch (7)；The setting of oxygen supply system
Bottom in bath, oxygen supply system connect source of oxygen；The medial surface in bath (3), bath (3) is arranged in heating plate (12)
Outside be provided with outlet (10).
2. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 1, which is characterized in that oxygen supplies
Answering system includes that unidirectional electric control valve (20), oxygen supply pipeline (14) and oxygen froth head (15), oxygen supply pipeline
It (14) include a main pipe rail and several bye-passes, bye-pass is uniformly arranged in bath (3) bottom, and oxygen is uniformly arranged on bye-pass
Head (15) are steeped in air-blowing；Unidirectional electric control valve (20) is arranged at the oxygen intake on main line, unidirectional electric control valve (20) connection
Control centre.
3. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 2, which is characterized in that warning lamp
Including heating lamp, thermostatical pilot lamp (6), oxygen warning lamp, pH value warning lamp and osmotic pressure indicator light, warning lamp connection
Control centre；It connects and is provided with overload protective device (8) on the way circuit of outlet (10).
4. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 2, which is characterized in that brain piece is contained
It is equal to put quantity and the oxygen quantity of head (15) that froths of (13) of slipping through the net, brain piece hold slip through the net (13) be located at oxygen and froth head (15)
Surface.
5. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 2, which is characterized in that bath
(3) bottom is provided with oxygen supply pipeline card slot (21), and oxygen supply pipeline (14) is arranged in the oxygen supply pipeline card
In slot (21).
6. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 2, which is characterized in that fixed plate
(2) total length is greater than the distance of the front and rear wall of bath (3), and the two sides of one end of fixed plate (2) offer gap, fixed plate
(2) it is uniformly provided with along its length and holds the hole of (13) of slipping through the net for installing brain piece.
7. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 1, which is characterized in that bath
(3) it is cube groove, is opened up U-type groove (22) on bath wall, is equipped with fixed plate rest stand (11) inside bath (3), U-type groove
(22) height of bottom surface is higher than fixed plate rest stand (11) top, and one end of fixed plate (2) is shelved on fixed plate rest stand (11)
On, handling section is caught in U-type groove (22), and handheld terminal is additionally provided with anti-drop structure.
8. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 7, which is characterized in that fixed plate
It (2) is bent structure, the bent structure includes handling section, linkage section and incubation section；It is incubated in section and is uniformly arranged brain piece
It holds slip through the net (13), handling section is parallel with section is incubated for.
9. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 7, which is characterized in that shading cover
It (1) is lighttight black lid, the bottom surface of shading cover (1) is additionally provided with screening corresponding with U-type groove size, shape and quantity
Mating plate.
10. the brain slice in vitro rewarming incubating device of real-time monitoring state according to claim 7, which is characterized in that bath
(3) insulating layer is provided on the outside of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910132954.0A CN109749929B (en) | 2019-02-22 | 2019-02-22 | Device is hatched to separation brain piece rewarming of real-time supervision state |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910132954.0A CN109749929B (en) | 2019-02-22 | 2019-02-22 | Device is hatched to separation brain piece rewarming of real-time supervision state |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109749929A true CN109749929A (en) | 2019-05-14 |
| CN109749929B CN109749929B (en) | 2022-08-19 |
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| CN113956977A (en) * | 2021-11-09 | 2022-01-21 | 上海交通大学 | Multifunctional bath for in vitro electrophysiological recording |
| CN114167042A (en) * | 2021-11-17 | 2022-03-11 | 上海交通大学 | Multifunctional platform for patch clamp recording and use method thereof |
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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