CN205205124U - Groove is hatched to porous brain piece - Google Patents
Groove is hatched to porous brain piece Download PDFInfo
- Publication number
- CN205205124U CN205205124U CN201521015016.6U CN201521015016U CN205205124U CN 205205124 U CN205205124 U CN 205205124U CN 201521015016 U CN201521015016 U CN 201521015016U CN 205205124 U CN205205124 U CN 205205124U
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- hole
- hatching
- brain
- groove
- hatch
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Abstract
The utility model discloses a groove is hatched to porous brain piece, it contains hatches the groove and holds box (1), and should hatch the groove and hold box (1) and go up one side of top surface and be provided with and detain complex top cap (2) rather than the lid, the groove of hatching hold the box and be provided with in (1) and hatch board (3) and be located base (5) of hatching below board (3), hatch and offer the brain piece that the several equidistance arranged on board (3) and hatch hole (4) and one oxygen hole (6), guiding gutter (11) of docking with adjacent side are seted up in the outside of hatching hole (4) and oxygen hole (6). The utility model discloses simple structure, it is relatively independent that the hole is hatched to the brain piece, is convenient for distinguish the order of brain piece, differentiates the festival section that the piece of requiring mental skill lies in brain district place interested, each brain piece is relatively independent, is difficult for receiving the disturbance, oxygenate the environment and in relatively independent oxygen hose, reduce the disturbance to the brain piece, it has sufficient space to hatch groove whole and hold the box bottom face, can ensure that the mechanical brains spinal fluid of oxygen saturation can freely circulate between the hole hatched to the brain piece.
Description
Technical field
The utility model relates to the processing and manufacturing field of Bioexperiment equipment, what be specifically related to is a kind of porous brain sheet incubation slot.
Background technology
Brain sheet (brainslice) is a kind of sample conventional in current Neuroscience Research, and method is by cerebral tissue or spinal cord is complete takes out in animal body, is cut into the thin slice of 200-500 μm in artificial cerebrospinal fluid.These thin slices remain activity and the function of partial nerve tissue.Compare with discrete or culturing cell, there is the advantage of the relatively complete neural circuitry of reservation and extracellular environment; Compare with body animal, have influence factor relatively simply, more controlled advantage.Usually can cut multiple brain sheets for interested brain district when preparing brain sheet, then brain sheet being placed in artificial cerebrospinal fluid and hatching.Common method is placed in the incubation slot of by all brain sheets to hatch.But each brain district configuration is also irregular, transition change can be had along different directions.Therefore, distinguish the function and form difference which sections that the brain sheet cut is positioned at whole brain district can help to understand brain district different segment in an experiment, have important meaning to the entirety understanding in interested brain district.In addition, brain sheet is to around culture environment is more responsive, and larger disturbance can have influence on the activity of brain sheet.The environment relative independentability of hatching of each brain sheet is kept to contribute to reducing the disturbance chosen a brain sheet and bring other brain sheet.
At present conventional brain sheet incubation slot mostly is all brain sheets cut to put together and hatches.The first, being difficult to accurately distinguish the brain sheet chosen is which sections being positioned at brain district interested, although can judge roughly according to general outline; The second, all brain sheets are mixed in together, when choose wherein a brain sheet time, be easy to produce disturbance to other brain sheet on side, this disturbance can upset the order that original brain sheet is placed on the one hand, obscures the hierarchical relationship that goes forward one by one of different brain sheet further.On the other hand, this disturbance can produce the neurone in brain sheet and neurogliocyte to stimulate, and causes the activation that neurone is unnecessary, has influence on neuronic activity and function.
Utility model content
The utility model object is to provide a kind of porous brain sheet incubation slot, and it can solve problem existing in background technology effectively.
In order to solve problem existing in background technology, it comprises incubation slot accommodation box 1, on this incubation slot accommodation box 1, the side of end face is provided with the top cover 2 coordinated with its cover buckle, be provided with in described incubation slot accommodation box 1 and hatch plate 3 and be positioned at the base 5 of hatching below plate 3, described brain sheet plate 3 offering several equidistant arrangement of hatching hatches hole 4 and an oxygen outlet 6, described outside of hatching hole 4 and oxygen outlet 6 offers the diversion trench 11 docked with adjacent side, described hatching between plate 3 with base 5 is connected by oxygen hose 7, one end of this oxygen hose 7 is docked with oxygen outlet 6, its the other end is fixed on base 5, described hatching in hole 4 is provided with silk screen screens circle 8, Bubbled stone 9 is placed with in described oxygen hose 7.
Described base 5 entirety is a rectangular platy structure, and the corner place of its bottom surface is separately installed with four footing 10.
The height of described silk screen screens circle 8 is contour with the height of hatching hole 4.
Owing to have employed above technical scheme, the utility model has following beneficial effect: structure is simple, and it is relatively independent that brain sheet hatches hole, is convenient to the order distinguishing brain sheet, differentiate sheet of requiring mental skill be positioned at the sections at place, brain district interested; Each brain sheet is relatively independent, is not easily disturbed; Oxygenation environment, in relatively independent oxygen hose, reduces the disturbance to brain sheet; The diversion trench of hole and oxygen outlet is hatched by each, and the gap under base, incubation slot is overall has enough spaces with accommodation box bottom surface, can guarantee that oxygen-saturated artificial cerebrospinal fluid can be hatched between hole at brain sheet and freely circulate.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.Apparently, the accompanying drawing in the following describes is only case study on implementation more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is structural representation of the present utility model.
Embodiment
The technique means realized to make the utility model, creation characteristic, reaching object and effect is easy to understand, below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment being clearly and completely described.
Referring to Fig. 1, this embodiment is achieved by the following technical solutions, it comprises incubation slot accommodation box 1, on this incubation slot accommodation box 1, the side of end face is provided with the top cover 2 coordinated with its cover buckle, be provided with in described incubation slot accommodation box 1 and hatch plate 3 and be positioned at the base 5 of hatching below plate 3, described brain sheet plate 3 offering several equidistant arrangement of hatching hatches hole 4 and an oxygen outlet 6, described outside of hatching hole 4 and oxygen outlet 6 offers the diversion trench 11 docked with adjacent side, described hatching between plate 3 with base 5 is connected by oxygen hose 7, one end of this oxygen hose 7 is docked with oxygen outlet 6, its the other end is fixed on base 5, described hatching in hole 4 is provided with silk screen screens circle 8, for blocking silk screen, brain sheet is placed on silk screen and hatches.Bubbled stone 9 is placed with in described oxygen hose 7.
Described base 5 entirety is a rectangular platy structure, and the corner place of its bottom surface is separately installed with four footing 10.
The height of described silk screen screens circle 8 is contour with the height of hatching hole 4.
Owing to have employed above technical scheme, this embodiment has following beneficial effect: structure is simple, and it is relatively independent that brain sheet hatches hole, is convenient to the order distinguishing brain sheet, differentiate sheet of requiring mental skill be positioned at the sections at place, brain district interested; Each brain sheet is relatively independent, is not easily disturbed; Oxygenation environment, in relatively independent oxygen hose, reduces the disturbance to brain sheet; The diversion trench of hole and oxygen outlet is hatched by each, and the gap under base, incubation slot is overall has enough spaces with accommodation box bottom surface, can guarantee that oxygen-saturated artificial cerebrospinal fluid can be hatched between hole at brain sheet and freely circulate.
Last it is noted that above embodiment is only in order to illustrate the technical solution of the utility model, be not intended to limit; Although be described in detail the utility model with reference to previous embodiment, those of ordinary skill in the art is to be understood that, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of each embodiment technical scheme of the utility model.
Claims (3)
1. a porous brain sheet incubation slot, it is characterized in that it comprises incubation slot accommodation box (1), the side of the upper end face of this incubation slot accommodation box (1) is provided with the top cover (2) coordinated with its cover buckle, be provided with in described incubation slot accommodation box (1) and hatch plate (3) and be positioned at the base (5) of hatching plate (3) below, described brain sheet plate (3) offering several equidistant arrangement of hatching hatches hole (4) and an oxygen outlet (6), described outside of hatching hole (4) and oxygen outlet (6) offers the diversion trench (11) docked with adjacent side, described hatching between plate (3) with base (5) is connected by oxygen hose (7), one end of this oxygen hose (7) is docked with oxygen outlet (6), its the other end is fixed on base (5), described hatching in hole (4) is provided with silk screen screens circle (8), Bubbled stone (9) is placed with in described oxygen hose (7).
2. a kind of porous brain sheet incubation slot according to claim 1, is characterized in that described base (5) entirety is a rectangular platy structure, and the corner place of its bottom surface is separately installed with four footing (10).
3. a kind of porous brain sheet incubation slot according to claim 1, the height that it is characterized in that described silk screen screens circle (8) is contour with the height of hatching hole (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521015016.6U CN205205124U (en) | 2015-12-05 | 2015-12-05 | Groove is hatched to porous brain piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521015016.6U CN205205124U (en) | 2015-12-05 | 2015-12-05 | Groove is hatched to porous brain piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205205124U true CN205205124U (en) | 2016-05-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521015016.6U Expired - Fee Related CN205205124U (en) | 2015-12-05 | 2015-12-05 | Groove is hatched to porous brain piece |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205205124U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108106912A (en) * | 2018-01-10 | 2018-06-01 | 复旦大学 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
-
2015
- 2015-12-05 CN CN201521015016.6U patent/CN205205124U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108106912A (en) * | 2018-01-10 | 2018-06-01 | 复旦大学 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
| CN109749929A (en) * | 2019-02-22 | 2019-05-14 | 中国人民解放军第四军医大学 | A kind of brain slice in vitro rewarming incubating device of real-time monitoring state |
| CN109749929B (en) * | 2019-02-22 | 2022-08-19 | 中国人民解放军第四军医大学 | Device is hatched to separation brain piece rewarming of real-time supervision state |
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
| CN114252324B (en) * | 2022-03-02 | 2022-05-13 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160504 Termination date: 20161205 |
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| CF01 | Termination of patent right due to non-payment of annual fee |