CN108106912A - Immunohistochemical staining experiment cleaning, which is protected from light, dries box - Google Patents
Immunohistochemical staining experiment cleaning, which is protected from light, dries box Download PDFInfo
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- CN108106912A CN108106912A CN201810023307.1A CN201810023307A CN108106912A CN 108106912 A CN108106912 A CN 108106912A CN 201810023307 A CN201810023307 A CN 201810023307A CN 108106912 A CN108106912 A CN 108106912A
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- layer
- incubation
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- cleaning
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- Pending
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- 238000004140 cleaning Methods 0.000 title claims abstract description 39
- 238000002474 experimental method Methods 0.000 title claims abstract description 11
- 238000011532 immunohistochemical staining Methods 0.000 title claims abstract description 8
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 22
- 238000011534 incubation Methods 0.000 claims description 38
- 239000011521 glass Substances 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000001727 in vivo Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 10
- 239000000428 dust Substances 0.000 abstract description 3
- 238000010166 immunofluorescence Methods 0.000 abstract description 2
- 238000010791 quenching Methods 0.000 abstract description 2
- 230000000171 quenching effect Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B9/00—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards
- F26B9/06—Machines or apparatus for drying solid materials or objects at rest or with only local agitation; Domestic airing cupboards in stationary drums or chambers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Optics & Photonics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to field of bioexperiment technology, and specially a kind of cleaning of immunohistochemical staining experiment, which is protected from light, dries box.Present invention cleaning, which is protected from light, dries box and includes：Shell, control panel are incubated cleaning device, airing device, moisturizing layer and battery plate layer；Wherein, shell is a rectangle box body, is incubated cleaning device, airing device, moisturizing layer and battery plate layer, may be contained in shell box body；Control panel is arranged at one side above shell box body；Above shell box body, it is adjacent with control panel, equipped with cover board, for covering box body；Control panel is equipped on ＆ off switch, temperature and humidity regulation button；Display screen is additionally provided with, for showing time, temperature and humidity.Present invention collection is protected from light, cleans, and dries three zones in one, can reduce the probability of fluorescent quenching, reduce the pollution of dust and prevent the dry and cracked of slice, thin piece, improve the clean-up performance of slice, thin piece, ensure the experiment effect of immunofluorescence.
Description
Technical field
The invention belongs to field of bioexperiment technology, and in particular to a kind of cleaning of immunohistochemical staining experiment is kept away
Light dries box.
Background technology
Immunohistochemical staining is most common experimental technique.Wherein, the whole process needs after secondary antibody are incubated to be protected from light.
At this stage, after being incubated secondary antibody, histotomy is placed on and is protected from light under can or platinum paper, this operation before especially being dried after mounting
Process is more inconvenient, because the operation dried can not only cause being quenched for fluorescence secondary antibody, also results in slice, thin piece and dries degree difference
（Some slice, thin pieces are dry and cracked, and some slice, thin pieces are still moist）And influence the mounting of slice, thin piece and the effect of dyeing.
Existing experiment material cannot be guaranteed to be incubated the effect that is protected from light after secondary antibody, cannot guarantee that the slice, thin piece dried it is clean,
Without dry and cracked, exactly there are it is such the problem of and defect, so a kind of technology is needed to improve the problem of such.
The content of the invention
It is protected from light it is an object of the invention to provide a kind of good cleaning of light-shading effect after incubation secondary antibody and dries box, to ensure to dry in the air
Dry slice, thin piece is clean, without dry and cracked.
The cleaning that the present invention designs, which is protected from light, dries box, including：Shell, control panel, incubation cleaning device, airing device,
Moisturizing layer and battery plate layer, structure are as shown in Figure 1.The shell is a rectangle box body, and the incubation cleaning device dries
Device, moisturizing layer and battery plate layer, may be contained in shell box body；Control panel is arranged at one side above shell box body；Shell box
Above body, it is adjacent with control panel, equipped with cover board 5, for covering box body.
The control panel is equipped on ＆ off switch, temperature and humidity regulation button etc..In addition, being additionally provided with display screen, it is used for
Show time, temperature and humidity.
The incubation cleaning device is arranged at the in vivo rear portion of box；Including sample cell, incubation slot and at least one rinse bath
（General 3）, both sample cell and incubation slot size are consistent, and sample cell is close together as in incubation slot during use；
Rinse bath is put into after sample cell is separated with incubation slot during cleaning；
The sample cell treats samples of incubation for placing；Incubation slot is used to store secondary antibody；During incubation, secondary antibody is put into incubation slot bottom
Portion, sample are put into sample cell, after sample cell is attached to incubation slot, cover cover board 5, you can start to be incubated；
For the rinse bath for holding cleaning solution PBS, lower part is equipped with osculum, and under non-working condition, osculum is sealed with rubber cap
It closes；
When needing cleaning, PBS will be full of in rinse bath, sample cell is moved to rinse bath, you can timing（Such as 8-10 minutes/
It is secondary, it is 2-3 times common）Cleaning.After having cleaned, PBS is discharged from osculum.
The airing device is protected from light layer 6 and moisturizing layer 7 and battery plate layer 8, and it is in vivo to be set in turn in box from top to bottom
Forepart；
The airing device includes several glass slides and a placing groove, and glass slide is detachable in placing groove；It can be incited somebody to action after patch
Glass slide is put into placing groove, shuts box cover 5, and adjustment time, humidity, temperature wait it to carry out mounting after drying（Generally room
Temperature, preferably humidity set to 1-25Br, 20 Br, preferably time 8-15 minute, 10 minutes）.
The moisturizing layer 7 is being dried below layer 6, and size is similar to drying layer, and interior filling deionized water plays moisturizing work
With slice, thin piece being avoided to dry.Specifically, ultra-pure water is added in moisturizing layer, is allowed to suitable humidity.
The battery plate layer 8 is below moisturizing layer 7, and for heating moisturizing layer, principle is as shown in Figure 2.Battery plate layer can control
Set temperature.
The shell, is made of high-temperature resistance plastice, and surrounding coating is protected from light material, and formation is protected from light layer, can so reduce light
Fluorescence is quenched in line.
The cleaning that the present invention designs, which is protected from light, dries box, and manipulation flow during use is：
（1）；During incubation, secondary antibody is put into incubation trench bottom, sample is put into sample cell, after sample cell is attached to incubation slot, lid
Upper cover plate 5, you can start to be incubated；Condition：Room temperature, incubation time are 50-70 minutes；
（2）After incubation, cleaning PBS is poured into rinse bath, the sample cell Jing Guo incubation process is taken out, is put into rinse bath
It is interior, after cleaning 2-3 times, PBS is discharged from osculum；
（3）Adjustment temperature, humidity, generally room temperature, humidity set to 1-25Br, preferably 20 Br, time 8-15 minute, preferably
10 minutes.Ultra-pure water is added in into moisturizing layer, after patch, glass slide is put into and is protected from light layer；Airing；Mounting.
Control panel can add time setting according to demand.
The cleaning that the present invention designs is protected from light the advantages of drying box：
（1）Collection is protected from light, cleans, and dries three zones in one, not only reduces the probability of fluorescent quenching, and reduce dust
It pollutes and prevents the dry and cracked of slice, thin piece.
（2）The setting of the buttons such as time, temperature, humidity mitigates dry and cracked, the damage of slice, thin piece, improves the cleaning journey of slice, thin piece
Degree ensures the effect of immunostaining.
I.e. present invention cleaning, which is protected from light, dries box, can reduce being quenched for fluorescence secondary antibody, increase the cleannes of slice, thin piece, so as to reduce
The impurity of slice, thin piece mitigates the pollution of dust in air, avoids the dry and cracked damage of slice, thin piece, ensures the experiment effect of immunofluorescence.
Description of the drawings
Fig. 1 is protected from light for present invention cleaning dries box structural diagrams.
Fig. 2 is the electrical schematic diagram of battery plate layer in the present invention.
Fig. 3 compares figure for effect.Wherein,（a）Room temperature, nothing dry slice, thin piece under the conditions of being protected from light,（b）After ten minutes, sample is done
Wet difference influences immunostaining effect.
Figure label：1 is switch, and 2 be display screen, and 3 be temperature, humidity regulation button, and 4 be handle, and 5 be cover board（It is protected from light
Lid）, 6 is are protected from light layer, and 7 be moisturizing layer, and 8 be battery plate layer, and 9 be drainpipe, and 10 be rinse bath, and 11 be incubation slot, and 12 be sample
Slot.
Specific embodiment
The present invention is further described below by specific embodiment.
The cleaning that the present invention designs, which is protected from light, dries box, and shell sizes are：Long 30cm* wide 22cm* high 4cm.It is divided into control
Faceplate part is incubated cleaning part, dries part, moisturizing layer and battery plate layer.
（1）The box case, can be used plastic production.Box body coating is protected from light material, and formation is protected from light layer.
（2）Control panel：Long 30cm* wide 2cm, equipped on ＆ off switch 1, temperature and humidity regulation button 3.In addition, it is additionally provided with
Display screen 2, for showing time, temperature and humidity etc..Cover board 5 plays the role of closing, is protected from light, to make conveniently moving, on cover board 5
Equipped with handle 4.
（3）It is incubated cleaning part：Incubation slot 11 be diameter 1.5cm, depth 3cm, bottom arc.Sample cell 12 and incubation slot
11 is separable, and size is identical, and complete note closes, and secondary antibody is put into 11 bottom of incubation slot during incubation, sample is put into sample cell 12, by sample
After product slot is attached to incubation slot, covers cover board 5 and be directly incubated.Rinse bath 10 is three, and size is identical, long 3cm*
Wide 20cm* depths 3cm, bottom is equipped with osculum, and when not working, osculum rubber cap closure, when cleaning will be full of in 3 slots
Sample slot 12 is moved to fixed time cleaning after rinse bath 10, discharges PBS through drainpipe 9 from osculum after cleaning by PBS.
（4）Dry part：This part is made of several 1.5cm*3.5cm*0.1cm glass slides placing grooves（Detachably）, patch
Glass slide after piece can be put into this slot, shut cover board 5, adjustment time, humidity, temperature wait it to carry out mounting after drying.
（5）Moisturizing layer 7：The lower section for drying layer is placed in, size is similar to drying layer, and interior filling deionized water plays guarantor
Wet effect.
（6）Battery plate layer 8：It is as shown in Figure 2 that it heats moisturizing principle.Circuit board principle, when temperature sensor experiences temperature
Less than setting value, electromagnet can attract connecting valve to be above connected to heating, and temperature, which reaches setting value, will disconnect heating company
It connects, recovers to humidification.
Fig. 3 compares figure for effect.Wherein, a）23 DEG C of room temperature, humidity 34%, under conditions of 10 minutes time, glass slide is placed in
In environment；b）After ten minutes, the sample dry and wet on glass slide is different, and some samples are dry and cracked apparent, and some samples are still moist, this
As a result mounting progress is not only influenced, also influences coloration result.
Claims (3)
1. a kind of immunohistochemical staining experiment cleaning, which is protected from light, dries box, which is characterized in that including：Shell, control panel,
It is incubated cleaning device, airing device, moisturizing layer and battery plate layer；Wherein：The shell is a rectangle box body, described to be incubated cleaning
Device, airing device, moisturizing layer and battery plate layer, may be contained in shell box body；Control panel is arranged at one above shell box body
Side；Above shell box body, it is adjacent with control panel, equipped with cover board, for covering box body；
The control panel is equipped on ＆ off switch, temperature and humidity regulation button；In addition, be additionally provided with display screen, during for showing
Between, temperature and humidity；
The incubation cleaning device is arranged at the in vivo rear portion of box；Including sample cell, incubation slot and at least one rinse bath, sample
Both product slot and incubation slot sizes are consistent, and sample cell is close together as in incubation slot during use；Sample cell during cleaning
Rinse bath is put into after being separated with incubation slot；
The sample cell treats samples of incubation for placing；Incubation slot is used to store secondary antibody；
For the rinse bath for holding cleaning solution PBS, lower part is equipped with osculum, and under non-working condition, osculum is sealed with rubber cap
It closes；
The airing device is protected from light layer and moisturizing layer and battery plate layer, is set in turn in the in vivo forepart of box from top to bottom；
The airing device includes several glass slides and a placing groove, and glass slide is detachable in placing groove；
The moisturizing layer is being dried below layer, and size is similar to drying layer, and interior filling deionized water plays moisture-keeping function；
The battery plate layer is below moisturizing layer, for heating moisturizing layer.
2. immunohistochemical staining experiment cleaning according to claim 1, which is protected from light, dries box, which is characterized in that described
Shell is made of high-temperature resistance plastice, and surrounding coating is protected from light material, and formation is protected from light layer.
3. immunohistochemical staining experiment cleaning according to claim 1, which is protected from light, dries box, which is characterized in that uses
When manipulation flow be：
（1）；During incubation, secondary antibody is put into incubation trench bottom, sample is put into sample cell, after sample cell is attached to incubation slot, lid
Upper cover plate, you can start to be incubated；Condition：Room temperature, incubation time are 50-70 minutes；
（2）After incubation, cleaning PBS is poured into rinse bath, the sample cell Jing Guo incubation process is taken out, is put into rinse bath
It is interior, after cleaning 2-3 times, PBS is discharged from osculum；
（3）Adjust temperature, humidity：Room temperature, humidity set to 1-25Br, time 8-15 minute；Ultra-pure water is added in into moisturizing layer, patch
After piece, glass slide is put into and is protected from light layer；Airing；Mounting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810023307.1A CN108106912A (en) | 2018-01-10 | 2018-01-10 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810023307.1A CN108106912A (en) | 2018-01-10 | 2018-01-10 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108106912A true CN108106912A (en) | 2018-06-01 |
Family
ID=62219587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810023307.1A Pending CN108106912A (en) | 2018-01-10 | 2018-01-10 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108106912A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111307560A (en) * | 2020-03-05 | 2020-06-19 | 同济大学 | Automatic urine cell staining device and control system |
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| CN202066731U (en) * | 2011-03-11 | 2011-12-07 | 复旦大学 | Instrument used for slicing and staining biological samples of electron microscope |
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| CN203275168U (en) * | 2013-06-09 | 2013-11-06 | 中南大学 | Special dyeing box for western blotting |
| CN203350255U (en) * | 2013-07-02 | 2013-12-18 | 武汉三鹰生物技术有限公司 | Adjustable horizontal incubation box |
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Cited By (1)
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| CN111307560A (en) * | 2020-03-05 | 2020-06-19 | 同济大学 | Automatic urine cell staining device and control system |
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