CN206858581U - A kind of brain slice in vitro incubation slot - Google Patents
A kind of brain slice in vitro incubation slot Download PDFInfo
- Publication number
- CN206858581U CN206858581U CN201720525905.XU CN201720525905U CN206858581U CN 206858581 U CN206858581 U CN 206858581U CN 201720525905 U CN201720525905 U CN 201720525905U CN 206858581 U CN206858581 U CN 206858581U
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- CN
- China
- Prior art keywords
- couveuse
- incubation
- door
- switch
- groove body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a kind of brain slice in vitro incubation slot, including being incubated groove body, rotor plate, screen pack, couveuse placing piece and couveuse barrel, the incubation slot body interior is provided with couveuse installing plate, the couveuse installing plate upper end is provided with couveuse, the couveuse is by couveuse placing piece, couveuse barrel, door rotary shaft, rotate door, door switch and couveuse main body composition, the incubation slot body side lower end is provided with water inlet pipe, the incubation groove body opposite side lower end is provided with water outlet pipe, the water inlet pipe and water outlet pipe are by connecting tube, conical flow guiding pipe and screen pack composition, the incubation groove body top is provided with incubation groove upper cap, the incubation groove upper cap is by rotor plate, fixed plate, rolling bearing, switch mount and switch form.The utility model can will be in vitro after brain piece be incubated, be effectively guaranteed the viability of cell, make that the research of brain tissue is more preferable, is more easily studied.
Description
Technical field
Incubation slot equipment technical field is the utility model is related to, specially a kind of brain slice in vitro incubation slot.
Background technology
Brain piece is a kind of sample commonly used in current Neuroscience Research, and method is completely from animal by brain tissue or spinal cord
Take out in vivo, be cut into 200-500 μm of thin slice in artificial cerebrospinal fluid, these thin slices remain partial nerve tissue activity and
Function, compared with discrete or culture cell, there is the advantages of retaining relatively complete neural circuitry and extracellular environment, preparing
Multiple brain pieces would generally be cut for brain area interested during brain piece, then brain piece is placed in artificial cerebrospinal fluid and incubated
To educate, common method is that all brain pieces are placed in the incubation slot of one to be incubated, however, present brain piece incubation slot,
Incubation that can only be one by one, it is therefore desirable to a kind of equipment that can be largely incubated.
Utility model content
The purpose of this utility model is to provide a kind of brain slice in vitro incubation slot, to solve what is proposed in above-mentioned background technology
Problem.
To achieve the above object, the utility model provides following technical scheme:A kind of brain slice in vitro incubation slot, including be incubated
Groove body, rotor plate, screen pack, couveuse placing piece and couveuse barrel, the incubation slot body interior are provided with incubation
Device installing plate, the couveuse installing plate lower end offer liquid flowing groove, and the couveuse installing plate upper end is provided with incubation
Device.
Wherein, the couveuse is by couveuse placing piece, couveuse barrel, door rotary shaft, rotation door, door switch and incubation
Device main body forms.
The incubation slot body side lower end is provided with water inlet pipe, and the incubation groove body opposite side lower end is provided with out
Pouring spout.
Wherein, the water inlet pipe and water outlet pipe form by connecting tube, conical flow guiding pipe and screen pack.
The incubation groove body top is provided with incubation groove upper cap.
Wherein, the incubation groove upper cap is made up of rotor plate, fixed plate, rolling bearing, switch mount and switch.
Preferably, the opening position for being incubated four angles in groove body bottom is mounted on support foot.
Preferably, bottom is provided with WR-200 temperature sensors among the incubation slot body interior.
Preferably, the couveuse placing piece is fixed on couveuse installing plate, is installed among the couveuse placing piece
There is couveuse main body.
Preferably, the couveuse barrel upper end side is provided with a rotary shaft, and rotation is provided with the door rotary shaft
Door, the rotation door top are provided with door switch.
Preferably, the screen pack is fixed on incubation slot body interior bottom, passes through taper on the outside of the screen pack
Mozzle connects connecting tube.
Preferably, the rotor plate is connected plate by rolling bearing, and rolling bearing is provided with two altogether, the rotation
Plate upper end is provided with switch mount, and the switch mount upper end is provided with switch.
Compared with prior art, the beneficial effects of the utility model are:The utility model scientific structure, reasonable, user
Just, efficiently, the utility model can conveniently carry out the incubation of multiple brain slice in vitro, can by water inlet pipe and water outlet pipe,
Increased WR-200 temperature sensors also in equipment, carry out the control of the humiture of inside, and this equipment can also pass through upper end
Groove upper cap is incubated, the brain piece for carrying out the incubation of inside is quickly picked and placeed, and makes the work of equipment easier and quick.
Brief description of the drawings
Fig. 1 is main structure diagram of the present utility model;
Fig. 2 is overlooking the structure diagram of the present utility model;
Fig. 3 is water inlet pipe of the present utility model and water outlet pipe main structure diagram;
Fig. 4 is couveuse main structure diagram of the present utility model.
In figure:1- support foots;2- is incubated groove body;3- liquid flowing grooves;4- water inlet pipes;5-WR-200 TEMPs
Device;6- is incubated groove upper cap;7- couveuse installing plates;8- couveuses;9- water outlet pipes;10- rotor plates;11- fixed plates;12- turns
Dynamic bearing;13- switch mounts;14- is switched;15- connecting tubes;16- conical flow guiding pipes;17- screen packs;18- couveuses are put
Put part;19- couveuse barrels;20- door rotary shafts;21- rotates door;22- door switch;23- couveuse main bodys.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Refer to Fig. 1-4, a kind of embodiment provided by the utility model:A kind of brain slice in vitro incubation slot, including incubation slot
Main body 2, rotor plate 10, screen pack 17, couveuse placing piece 18 and couveuse barrel 19, it is incubated inside groove body 2 and is provided with
Couveuse 8 can be placed on upper end by couveuse installing plate 7, couveuse installing plate 7, picked and placeed brain piece sample more convenient, incubated
Educate the lower end of device installing plate 7 and offer liquid flowing groove 3, liquid flowing groove 3 can be flowed for liquid, to control inside
Temperature and humidity, the upper end of couveuse installing plate 7 are provided with couveuse 8, and couveuse 8 can deposit brain piece sample, and couveuse 8 is by incubating
Educate device placing piece 18, couveuse barrel 19, door rotary shaft 20, rotation door 21, door switch 22 and couveuse main body 23 to form, be incubated
The side lower end of groove body 2 is provided with water inlet pipe 4, is incubated the opposite side lower end of groove body 2 and is provided with water outlet pipe 9, water inlet pipe 4
Formed with water outlet pipe 9 by connecting tube 15, conical flow guiding pipe 16 and screen pack 17, incubation groove body 2 top, which is provided with, incubates
Educate groove upper cap 6, picking and placeing for brain piece sample can be facilitated by being incubated groove upper cap 6, be incubated groove upper cap 6 by rotor plate 10, fixed plate 11, turn
Dynamic bearing 12, switch mount 13 and the composition of switch 14.
The opening position for being incubated four angles in bottom of groove body 2 is mounted on support foot 1, is incubated the bosom bottom of groove body 2
End is provided with WR-200 temperature sensors 5, and WR-200 temperature sensors 5 can monitor the temperature and humidity of inside in real time, be incubated
Device placing piece 18 is fixed on couveuse installing plate 7, and couveuse main body 23, couveuse cylinder are provided among couveuse placing piece 18
The upper end side of wall 19 is provided with a rotary shaft 20, is provided with door rotary shaft 20 and rotates door 21, rotates the top of door 21 and is provided with door
Switch 22, screen pack 17, which is fixed on, is incubated the inner bottom of groove body 2, and screen pack 17 can be filtered liquid, be controlled
The clean-up performance of internal liquid, the outside of screen pack 17 connect connecting tube 15 by conical flow guiding pipe 16, and rotor plate 10 passes through
Rolling bearing 12 is connected plate 11, and rolling bearing 12 is provided with two altogether, and the upper end of rotor plate 10 is provided with switch mount 13,
The upper end of switch mount 13 is provided with switch 14.
Specifically used mode:In utility model works, user is first fixed on the table by equipment, and water inlet pipe is connected to
On water inlet pipe 4, then outlet pipe will be connected on water outlet pipe 9, you can start to be incubated work.
First, the rotation door 21 of couveuse main body 23 is rotated around door rotary shaft 20, puts brain piece tissue into incubation
Inside device barrel 19, then by rotate door 21 shut, finally, rotor plate 10 is opened, couveuse main body 23 is put into couveuse
Among couveuse placing piece 18 on installing plate 7, then rotor plate 10 shut, cultivated, inside liquid flowing groove 3
Liquid is flowed, and adjusts the temperature and humidity of inside, and so far, the workflow of whole equipment is completed.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and
And in the case of without departing substantially from spirit or essential attributes of the present utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new
The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained
All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation
Involved claim.
Claims (7)
1. a kind of brain slice in vitro incubation slot, including be incubated groove body (2), rotor plate (10), screen pack (17), couveuse and place
Part (18) and couveuse barrel (19), it is characterised in that:Couveuse installing plate (7) is installed inside the incubation groove body (2),
Couveuse installing plate (7) lower end offers liquid flowing groove (3), and couveuse installing plate (7) upper end is provided with incubation
Device (8);
Wherein, the couveuse (8) is by couveuse placing piece (18), couveuse barrel (19), door rotary shaft (20), rotation door
(21), door switch (22) and couveuse main body (23) composition;
Described incubation groove body (2) side lower end is provided with water inlet pipe (4), described incubation groove body (2) opposite side lower end peace
Equipped with water outlet pipe (9);
Wherein, the water inlet pipe (4) and water outlet pipe (9) are by connecting tube (15), conical flow guiding pipe (16) and screen pack
(17) form;
Described incubation groove body (2) top, which is provided with, is incubated groove upper cap (6);
Wherein, the incubation groove upper cap (6) is by rotor plate (10), fixed plate (11), rolling bearing (12), switch mount (13)
With switch (14) composition.
A kind of 2. brain slice in vitro incubation slot according to claim 1, it is characterised in that:Described incubation groove body (2) bottom
The opening position at four angles is mounted on support foot (1).
A kind of 3. brain slice in vitro incubation slot according to claim 1, it is characterised in that:The incubation groove body (2) is internal
Middle bottom is provided with WR-200 temperature sensors (5).
A kind of 4. brain slice in vitro incubation slot according to claim 1, it is characterised in that:The couveuse placing piece (18) is solid
It is scheduled on couveuse installing plate (7), couveuse main body (23) is installed among the couveuse placing piece (18).
A kind of 5. brain slice in vitro incubation slot according to claim 1, it is characterised in that:Couveuse barrel (19) upper end
Side is provided with a rotary shaft (20), is provided with the door rotary shaft (20) and rotates door (21), described rotation door (21) top
Door switch (22) is installed.
A kind of 6. brain slice in vitro incubation slot according to claim 1, it is characterised in that:The screen pack (17) is fixed on
Groove body (2) inner bottom is incubated, passes through conical flow guiding pipe (16) connection connecting tube (15) on the outside of the screen pack (17).
A kind of 7. brain slice in vitro incubation slot according to claim 1, it is characterised in that:The rotor plate (10) passes through rotation
Bearing (12) is connected plate (11), and rolling bearing (12) is provided with two altogether, and rotor plate (10) upper end is provided with switch
Installed part (13), switch mount (13) upper end are provided with switch (14).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720525905.XU CN206858581U (en) | 2017-05-12 | 2017-05-12 | A kind of brain slice in vitro incubation slot |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720525905.XU CN206858581U (en) | 2017-05-12 | 2017-05-12 | A kind of brain slice in vitro incubation slot |
Publications (1)
Publication Number | Publication Date |
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CN206858581U true CN206858581U (en) | 2018-01-09 |
Family
ID=60824020
Family Applications (1)
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CN201720525905.XU Expired - Fee Related CN206858581U (en) | 2017-05-12 | 2017-05-12 | A kind of brain slice in vitro incubation slot |
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CN (1) | CN206858581U (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108106912A (en) * | 2018-01-10 | 2018-06-01 | 复旦大学 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
CN109085029A (en) * | 2018-07-03 | 2018-12-25 | 复旦大学 | A kind of isolated preparation nutrient solution discontinuous flow device excluding outer signals interference |
CN110607235A (en) * | 2018-06-14 | 2019-12-24 | 复旦大学 | Electric heating constant-temperature isolated organ incubator |
CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
-
2017
- 2017-05-12 CN CN201720525905.XU patent/CN206858581U/en not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108106912A (en) * | 2018-01-10 | 2018-06-01 | 复旦大学 | Immunohistochemical staining experiment cleaning, which is protected from light, dries box |
CN110607235A (en) * | 2018-06-14 | 2019-12-24 | 复旦大学 | Electric heating constant-temperature isolated organ incubator |
CN109085029A (en) * | 2018-07-03 | 2018-12-25 | 复旦大学 | A kind of isolated preparation nutrient solution discontinuous flow device excluding outer signals interference |
CN109085029B (en) * | 2018-07-03 | 2020-11-10 | 复旦大学 | Isolated specimen nutrient solution intermittent flow device capable of eliminating external signal interference |
CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180109 Termination date: 20180512 |