CN114252324A - Device is hatched to porous brain piece that facilitates use - Google Patents
Device is hatched to porous brain piece that facilitates use Download PDFInfo
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- CN114252324A CN114252324A CN202210194941.8A CN202210194941A CN114252324A CN 114252324 A CN114252324 A CN 114252324A CN 202210194941 A CN202210194941 A CN 202210194941A CN 114252324 A CN114252324 A CN 114252324A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention relates to a conveniently-used porous brain slice incubation device, which comprises an incubation device body; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage is communicated with the incubation chamber, and the oxygen passage is connected with an oxygen supply pipeline; the incubation chamber is internally provided with an incubation plate which is movably arranged inside the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for bearing brain slices/tissue slices is arranged below the incubation hole; the incubation chamber is internally provided with a separator which divides the incubation chamber into different spaces; a temperature control device is arranged outside the incubation device body, and the temperature control device still uses the principle of a water bath method; the temperature can be well kept by incubation under the water bath condition, and the temperature is controlled and heated uniformly; placing the brain slice/tissue slice in the incubation hole in such a way, providing oxygen for the brain slice/tissue slice by using the oxygen supply pipeline and the oxygen passage, and incubating; the isolation piece is arranged, so that the waste of incubation liquid during incubation of a single brain slice can be effectively avoided.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a convenient porous brain slice incubation device.
Background
The brain slice is a specimen commonly used in neuroscience research at present, and the method is that the brain tissue or spinal cord is completely taken out from the animal body, and is cut into 200-500 mu m slices in artificial cerebrospinal fluid. These sheets retain the activity and function of a portion of the neural tissue, and have the advantage of retaining a relatively intact neural circuit and extracellular environment compared to discrete or cultured cells; compared with the animal body, the method has the advantages of relatively simple and controllable influence factors, a plurality of brain slices are usually cut aiming at the interested brain area when the brain slices are prepared, then the brain slices are placed in the artificial cerebrospinal fluid for incubation, and the common method is to place all the brain slices in one incubation barrel for incubation. However, the overall shape of each brain area is not regular, and may change in a transition manner in different directions. Therefore, distinguishing which section of the whole brain area the cut brain slice is located in the experiment can help to know the function and shape difference of different sections of the brain area, and has important significance for overall understanding of the brain area of interest.
At present, most of the commonly used brain slice incubation barrels place all the cut brain slices together for incubation. Such incubation has the following drawbacks: 1. all brain slices are mixed together, when one brain slice is selected, other brain slices beside the brain slice are easily disturbed, and the disturbance disturbs the placement sequence of the original brain slices on one hand and further confuses the progressive hierarchical relationship of different brain slices; on the other hand, the disturbance can stimulate neurons and glial cells in brain slices, cause unnecessary activation of the neurons, and influence the activity and function of the neurons; 2. at present, the brain slice incubation temperature is mostly carried out under two conditions of 34 ℃ and room temperature, but at present, the temperature is mostly kept under the water bath condition, although the water bath temperature control is uniform in heating and convenient to observe, when the water bath temperature control is used, once the water level is lower than that of the incubator, the incubator can be inclined, so that the normal brain slice incubation is influenced; if a new incubation liquid is added, the temperature can float, the activity of the brain slice/tissue slice is influenced, and the experimental result is influenced; 3. the existing brain slice incubation structure is an instrument for incubating a plurality of brain slices, and when the brain slices are put together, the brain slices are easily overlapped to influence the activity; 4. when a single brain piece/tissue piece is incubated in the incubator, the incubation liquid is wasted.
Aiming at the problems, the invention provides the conveniently-used porous brain slice incubation device, which effectively ensures a proper incubation environment and simultaneously solves the problems that a plurality of incubations can be incubated together, and a single incubation can effectively save incubation liquid.
Disclosure of Invention
In order to overcome the problems presented in the background art, the present invention provides a convenient multi-well brain sheet incubating device.
A convenient to use porous brain piece incubation device, comprising an incubation device body; an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage is communicated with the incubation chamber, and the oxygen passage is connected with an oxygen supply pipeline; the incubation chamber is internally provided with an incubation plate which is movably arranged in the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for bearing brain slices/tissue slices is arranged below the incubation hole; arranging a partition piece in the incubation chamber, wherein the partition piece divides the interior of the incubation chamber into different spaces; a temperature control device is arranged outside the incubation device body, and the temperature control device still uses the principle of a water bath method; the temperature can be well kept by incubation under the water bath condition, and the temperature is controlled and heated uniformly, so that a proper temperature environment is ensured; placing the brain slice/tissue slice in the incubation hole in such a way, providing oxygen for the brain slice/tissue slice by using the oxygen supply pipeline and the oxygen passage, and incubating; the isolation piece is arranged, so that the waste of incubation liquid during incubation of a single brain slice can be effectively avoided.
Furthermore, a cover plate is arranged at the upper end of the incubation device body, and the cover plate covers the incubation device body to ensure the sealing performance of the incubation chamber and the oxygen passage.
Further, the first end of the oxygen passage is arranged at the lower end of the incubation chamber, the second end of the oxygen passage is arranged at the left end or the right end of the incubation chamber, and the oxygen passage and the incubation chamber form a closed-loop square; the arrangement can drive the flow of the incubation liquid, but the oxygen loop is not arranged on the incubation plate, so that the blowing-off force to the brain slice/tissue slice is prevented.
Further, the incubation net is made of nylon yarn so as to ensure normal incubation of brain slices/tissue slices; the incubation plate is made of acrylic materials.
Furthermore, a baffle strip is arranged on the inner side wall of the incubation chamber, and an incubation plate is placed at the upper end of the baffle strip; the shelves strip is used for the fixed position of hatching the board in hatching indoor, guarantees to hatch the stability of board. The stop strip is at a distance from the upper edge of the incubation chamber.
Furthermore, two or more than two incubation holes are arranged, a single brain slice/tissue slice is placed in a single incubation hole, and the brain slice/tissue slice is taken when the brain slice/tissue slice is taken. The arrangement of the plurality of incubation holes effectively solves the problems that the brain slices/tissue slices are mixed together, are easy to overlap, are poor in nutrition supply and the like at present, for example, when one brain slice is selected, the adjacent brain slices/tissue slices are easy to be disturbed.
Further, the isolation piece comprises a first partition plate positioned in the incubation chamber, a second partition plate positioned in the oxygen passage, and a support plate arranged on the inner wall of the oxygen passage; the first partition plate and the second partition plate are perpendicular to each other, the first partition plate partitions the interior of the incubation chamber, the second partition plate partitions the oxygen passage, and the incubation liquid is placed in the chamber for placing the brain slice/tissue slice; the incubation plate is provided with a through hole for accommodating the first partition plate; when the device is used, the second partition plate is placed at the upper end of the annular plate, the first partition plate is located in the incubation chamber, the through hole is aligned to the first partition plate, the incubation plate moves downwards, and the first partition plate partitions the incubation plate.
Furthermore, a third partition plate is arranged in the area where the brain piece/tissue piece is not planned to be incubated, and covers the incubation hole where the brain piece/tissue piece is not planned to be incubated.
Further, a temperature sensing piece is arranged in the oxygen passage and/or the incubation chamber, and the temperature of the incubation liquid is monitored in real time; the problem that only the temperature of the water bath solution can be monitored when the temperature is controlled by adopting a water bath method at present is solved, and more accurate temperature conditions are provided for the incubation of brain slices/tissue slices.
Further, the temperature control device comprises a first area for accommodating the incubation device body and the water bath solution, and a second area positioned below the first area, wherein the first area and the second area are separated by a separation baffle; a regulating part for regulating the water level of the water bath liquid in the first area is arranged in the second area; this kind of setting up of water line with adjustment water bath liquid can guarantee to be continuously taking brain piece/tissue piece after, it reduces to incubate indoor incubation liquid, can not satisfy the condition of hatching the requirement, satisfy the water bath demand through the mode that directly improves the water line of current water bath liquid this moment, avoid again to hatching indoor new incubation liquid that adds, temperature environment can change on the one hand, be unfavorable for the hatching of brain piece/tissue piece, on the other hand also can take place the disturbance to the position of brain piece/tissue piece that has placed, also be in order to avoid appearing the water bath water line and be less than the indoor incubation liquid of hatching simultaneously, the condition of slope takes place for the incubator, thereby influence the normal incubation of brain piece/tissue piece.
Further, the regulating member comprises: the movable plate is positioned in the second area, the support frame supports the movable plate, and the operating parts are arranged on the movable plate and the support frame; the lower ends of the two sides of the movable plate are provided with connecting plates, and the support frame is provided with connecting grooves for the connecting plates to extend into and move; the support frame is fixed in the second area; the operation part controls the connecting plate and the moving plate to move relative to the supporting frame, and the water bath liquid moves the upper part of the moving plate in the first area and the second area. The upper end of the movable plate is provided with an electric heating wire to ensure the temperature of the water bath liquid.
Furthermore, slotted holes are arranged in the upper part and the lower part of the partition baffle in a penetrating way, so that the upper end space of the movable plate in the second area is communicated with the first area; when the water level line of the water bath liquid descends, the moving plate moves upwards, the water bath liquid moves upwards through the slotted hole, and the water level line is ensured; when the water line of the water bath solution is too high, the moving plate moves downwards, and the water bath solution moves upwards through the slotted hole.
Furthermore, a first connecting block is arranged above the separation baffle, and a second connecting block matched with the first connecting block is also arranged at the lower end of the incubation device body; the incubation device body is stably fixed in the first area by the aid of the first connecting block and the second connecting block, and the situation that the incubation device body moves upwards is avoided. The first connecting block is arranged into an inverted L shape or a T shape or other shapes with the upper parts capable of playing a blocking role, and the second connecting block is matched with the first connecting block in shape.
Furthermore, the operating part comprises a first connecting shaft arranged between the supporting frames on the two sides and a second connecting shaft arranged between the connecting plates on the two sides, a first rotating wheel is arranged on the outer side of the first connecting shaft, a second rotating wheel is arranged at the corresponding position on the outer side of the second connecting shaft, and the relative positions of the first rotating wheel and the second rotating wheel are fixed; the circle center of the first connecting shaft is not coincident with the circle center of the first rotating wheel, and the circle center of the second connecting shaft is not coincident with the circle center of the second rotating wheel; the first connecting shaft is movably connected with the support frame, and the second connecting shaft is movably connected with the connecting plate; a driving part is arranged on one side or both sides of the first connecting shaft; the first connecting shaft and the first rotating wheel are driven to rotate through the operation driving portion, the first rotating wheel and the second rotating wheel are driven to rotate through rotation, and the first rotating wheel and the second rotating wheel are eccentrically arranged relative to the first connecting shaft and the second connecting shaft respectively, so that the first rotating wheel and the second rotating wheel can drive the connecting plate and the moving plate to move up and down when rotating relatively. The first rotating wheel and the second rotating wheel have the same diameter.
Furthermore, two ends of the first rotating wheel or the second rotating wheel are provided with protruding rings which just accommodate the second rotating wheel or the first rotating wheel; the arrangement ensures the stability of the first rotating wheel and the second rotating wheel when the first rotating wheel and the second rotating wheel rotate mutually.
Furthermore, the first rotating wheel/the second rotating wheel are respectively provided with 2 wheels which are symmetrically arranged at two ends, so that the stability of the first connecting shaft and the second connecting shaft when the first connecting shaft and the second connecting shaft rotate is ensured.
Furthermore, a first gear is arranged on the outer side of the first connecting shaft, a second gear is arranged on the outer side of the second connecting shaft, and the first gear and the second gear are meshed with each other; the center of the first gear and the center of the first rotating wheel are horizontally mapped into a point, and the center of the second gear and the center of the second rotating wheel are horizontally mapped into a point; the self-locking effect is effectively realized by the arrangement of the first gear and the second gear.
Furthermore, the driving part is a rotating handle arranged on one side or two sides of the connecting shaft, and the manual rotating mode is convenient to control.
Or the driving part is a motor arranged on one side or two sides of the connecting shaft, and a fixing frame for supporting the motor is arranged on the outer side of the motor; a protection box with a protection function is arranged on one side or two sides of the temperature control device; the electric control mode is time-saving and labor-saving, and the automation is completed.
Furthermore, a control piece is arranged on the outer surface of the temperature control device, and the control piece is provided with a temperature adjusting button for controlling the temperature of the water bath, a constant temperature indicator lamp, a heating indicator lamp and a time display screen.
Further, the control part is also provided with an operation key for controlling the starting/closing of the motor, so that the operation is convenient.
The invention has the beneficial effects that: 1. by arranging the incubation plate with a plurality of incubation holes and the closed-loop type oral oxygen passage and the incubation chamber, the problems that the brain slices/tissue slices float and overlap, the oxygenation degree of incubation liquid is poor, the activity of cells in the in-vitro brain slices/tissue slices is fully maintained, the survival rate is improved, and a good experimental result is obtained; 2. the isolation piece is arranged in the incubation chamber, the incubation chamber is divided into two spaces, a unique separated double chamber can incubate samples from different sources at the same time, and can also perform different treatments on the samples from the same source, so that the problem that the conventional brain slice incubation device only has one incubation groove and cannot perform comparative observation tests on two experimental treatment conditions at the same time is solved; meanwhile, the problem of waste of incubation liquid when a single brain piece/tissue piece is incubated is also solved; 3. the incubation device body is provided with the temperature control device, and the liquid level height of the water bath liquid can be adjusted, so that the water level line of the water bath liquid outside the incubation device is stable, and the survival rate and the activity of the incubated diaper are improved; in addition, the temperature sensing piece is arranged in the incubation chamber and/or the oxygen passage, so that the temperature of the incubation liquid can be monitored, the problem that the existing water bath kettle only can monitor the temperature of water is solved, and the incubation liquid really influences the rewarming incubation effect.
Drawings
FIG. 1 is a schematic view of the overall structure of the incubation device body according to the present invention;
FIG. 2 is a schematic view of the whole structure of the incubation device body without a cover plate;
FIG. 3 is a front perspective view of the body of the incubating device without a cover plate;
FIG. 4 is an enlarged schematic view of the upper end of the body of the incubation apparatus of the present invention;
FIG. 5 is a schematic top view of an incubation plate according to the present invention;
FIG. 6 is a schematic view of the bottom of the plate according to the present invention;
FIG. 7 is a perspective view of the whole structure of the incubation device body with a partition in the incubation chamber according to the present invention;
FIG. 8 is a sectional view of a partial structure of an incubation device body with a partition in an incubation chamber according to the present invention;
FIG. 9 is a schematic view of the structure of the incubation plate and spacer of the present invention;
FIG. 10 is a schematic structural view of a temperature control device disposed outside the body of the incubation device of the present invention;
FIG. 11 is a perspective view of a temperature control device disposed outside the body of the incubation device of the present invention;
FIG. 12 is an enlarged sectional view of the lower end of the temperature control device according to the present invention;
FIG. 13 is an enlarged schematic view of the lower end of the body of the incubation device and the adjustment member (the operation part is a rotary handle) according to the present invention;
FIG. 14 is a schematic structural view of the incubation device body and the temperature control device (the control element and the protection box are arranged outside the temperature control device) according to the present invention;
FIG. 15 is a schematic structural diagram of the adjusting member (the operation portion is provided as a motor) according to the present invention;
in the figure, 1, an incubation apparatus body; 11. an incubation chamber; 12. an oxygen pathway; 13. an oxygen supply line; 14. incubating the plate; 15. incubating the wells; 16. incubating the mesh; 17. a cover plate; 18. blocking strips; 19. a first partition plate; 110. a second partition plate; 111. a support plate; 112. a through hole; 113. a third partition plate; 114. a temperature sensing sheet; 2. a temperature control device; 21. a first region; 22. a second region; 23. a separation baffle; 24. moving the plate; 25. a support frame; 26. a connecting plate; 27. an electric heating wire; 28. a first connecting block; 29. a second connecting block; 210. a first connecting shaft; 211. a second connecting shaft; 212. rotating the first wheel; 213. a second rotating wheel; 214. a first gear; 215. a second gear; 216. rotating the handle; 217. a motor; 218. a fixed mount; 219. a protective case; 220. and (6) a control member.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below by specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and other advantages and effects of the present invention can be easily understood by those skilled in the art from the disclosure of the present specification. The present invention can be implemented or applied by other different specific embodiments, and the features in the following embodiments and embodiments can be combined with each other without conflict, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
Referring to fig. 1 to fig. 3, a convenient multi-hole brain slice incubating device in this embodiment includes an incubating device body 1; an incubation chamber 11 and an oxygen passage 12 are arranged in the incubation device body 1, the oxygen passage 12 is communicated with the incubation chamber 11, and the oxygen passage 12 is connected with an oxygen supply pipeline 13; an incubation plate 14 is arranged in the incubation chamber 11, and the incubation plate 14 is movably arranged in the incubation chamber 11; the incubation plate 14 is provided with at least one incubation hole 15, and an incubation net 16 for bearing brain slices/tissue slices is arranged below the incubation hole 15; a partition is arranged in the incubation chamber 11 and divides the inside of the incubation chamber 11 into different spaces; a temperature control device 2 is arranged outside the incubation device body 1, and the temperature control device 2 still uses the principle of a water bath method; the temperature can be well kept by incubation under the water bath condition, and the temperature is controlled and heated uniformly, so that a proper temperature environment is ensured; the brain slice/tissue slice is placed in the incubation hole 15 in this way, oxygen is provided for the brain slice/tissue slice by the oxygen supply pipeline 13 and the oxygen passage 12, and the brain slice/tissue slice is incubated; the isolation piece is arranged, so that the waste of incubation liquid during incubation of a single brain slice can be effectively avoided.
Example 2
Referring to fig. 1 to 6, on the basis of embodiment 1, the following specific settings are performed on the incubation device body 1 in this embodiment: a cover plate 17 is provided at the upper end of the incubator main body 1, and the cover plate 17 covers the incubator main body 1 to ensure the sealing property of the incubation chamber 11 and the oxygen passage 12. The shape of the incubator body 1 is not limited, and in the present embodiment, the incubator body 1 is formed in a square shape, but the incubator body 1 may be formed in a cylindrical shape or a shape that can be smoothly implemented. The shape of the incubation hole 15 is not limited, and the incubation hole 15 in this embodiment may be square, circular or other convenient shapes. The shapes of the incubation chamber 11 and the incubation plate 14 are matched, in this embodiment, the shapes of the incubation chamber 11 and the incubation plate 14 are both square, and the shapes of the incubation chamber 11 and the incubation plate 14 can also be circular or other shapes convenient to use, and are not limited thereto.
The first end of the oxygen passage 12 is arranged at the lower end of the incubation chamber 11, the second end of the oxygen passage 12 is arranged at the left end or the right end of the incubation chamber 11, and the oxygen passage 12 and the incubation chamber 11 form a closed-loop square shape; in the present embodiment, the oxygen supply line 13 is disposed at any section of the oxygen passage 12 parallel to the center line of the incubation chamber 11; when in use, the oxygen supply pipeline 13 is connected with an oxygen supply apparatus, and oxygen enters the oxygen passage 12 to move upwards and enters the incubation chamber 11 along the oxygen passage 12 to provide oxygen for the incubation of brain slices/tissue slices. This arrangement allows the flow of the incubation liquid, but the oxygen return path is not on the plate 14, preventing blow-off forces on the brain/tissue plate. A barrier strip 18 is arranged on the inner side wall of the incubation chamber 11, and the incubation plate 14 is placed at the upper end of the barrier strip; the stop strip is used to fix the position of the incubation plate 14 in the incubation chamber 11, so as to ensure the stability of the incubation plate 14. The stop strip is spaced from the upper edge of the incubation chamber 11.
Two or more than two incubation holes 15 are arranged, a single brain piece/tissue piece is placed in the single incubation hole 15, and the brain piece/tissue piece is taken when the brain piece/tissue piece is taken. The arrangement of the plurality of incubation holes 15 effectively solves the problems that the brain slices/tissue slices are mixed together, are easy to overlap, are not good in nutrition and the like at present, for example, when one brain slice is selected, the adjacent brain slices/tissue slices are easy to be disturbed. The incubation net 16 is made of nylon yarn to ensure normal incubation of brain slices/tissue slices; the incubation plate 14 is made of acrylic material.
The control member 220 is provided on the outer surface of the temperature control device 2, and the control member 220 is further provided with an operation key for controlling the motor 217 to be turned on/off for easy operation.
Example 3
Referring to fig. 7 to 9, on the basis of embodiment 2, the present embodiment specifically sets the spacers: the partition in this embodiment divides the inside of the incubation chamber 11 into different spaces. The partition comprises a first partition plate 19 positioned in the incubation chamber 11, a second partition plate 110 positioned in the oxygen passage 12, and a support plate 111 arranged on the inner wall of the oxygen passage 12; the first partition plate 19 and the second partition plate 110 are vertical to each other, the first partition plate 19 partitions the interior of the incubation chamber 11, the second partition plate 110 partitions the oxygen passage 12, and the incubation liquid is placed in a cavity for placing brain slices/tissue slices; the incubation plate 14 is provided with a through hole 112 for accommodating the first partition plate 19; when the incubator is used, the second partition plate 110 is placed at the upper end of the annular plate, the first partition plate 19 is positioned in the incubation chamber 11, the through hole 112 is aligned with the first partition plate 19, the incubation plate 14 moves downwards, and the first partition plate 19 partitions the incubation plate 14.
The third partition plate 113 is arranged in the area where the brain piece/tissue piece is not planned to be incubated, and covers the incubation hole 15 where the brain piece/tissue piece is not planned to be incubated. In this embodiment, the oxygen passage 12 is configured to be circular, the support plate 111 is configured to be an annular plate, the brain slice/tissue slice is incubated on the side away from the right side of the square-shaped oxygen passage 12, the partition plate two 110 is configured to be semicircular, the length of the partition plate one 19 at the upper end portion of the incubation plate 14 is smaller than the length at the lower end portion of the incubation plate 14, so that the incubation liquid can flow into the side on which the brain slice/tissue slice is incubated, and the partition plate three 113 blocks the incubation liquid from flowing into the incubation chamber 11 from the oxygen passage 12 and blocks the incubation liquid from flowing into the incubation chamber 11 from the incubation hole 15.
A temperature sensing piece 114 is arranged in the oxygen passage 12 and/or the incubation chamber 11, so that the temperature of the incubation liquid is monitored in real time; the problem that only the temperature of the water bath solution can be monitored when the temperature is controlled by adopting a water bath method at present is solved, and more accurate temperature conditions are provided for the incubation of brain slices/tissue slices.
Example 4
Referring to fig. 10 to fig. 15, on the basis of embodiment 1, 2 or 3, the present embodiment specifically sets the temperature control device 2: the temperature control device 2 comprises a first area 21 for accommodating the incubation device body 1 and the water bath solution, and a second area 22 positioned below the first area 21, wherein the first area 21 and the second area 22 are separated by a separation baffle 23; a regulating part for regulating the water level of the water bath liquid in the first area 21 is arranged in the second area 22; the arrangement of the water line with the water bath liquid can ensure that after brain slices/tissue slices are continuously taken, the incubation liquid in the incubation chamber 11 is reduced, and the incubation requirement cannot be met, at the moment, the water line of the existing water bath liquid is directly increased to meet the water bath requirement, and the situation that new incubation liquid is added into the incubation chamber 11 again is avoided, on one hand, the temperature environment is changed, so that the brain slices/tissue slices are not incubated favorably, on the other hand, the positions of the placed brain slices/tissue slices are disturbed, and on the other hand, the situation that the water line of the water bath liquid is lower than the incubation liquid in the incubation chamber 11, and the incubator is inclined is avoided, so that the normal incubation of the brain slices/tissue slices is influenced.
The regulating part comprises: a moving plate 24 located in the second region 22, a support frame 25 supporting the moving plate 24, and an operating portion provided on the moving plate 24 and the support frame 25; the lower ends of the two sides of the moving plate 24 are provided with connecting plates 26, and the supporting frame 25 is provided with connecting grooves for the connecting plates 26 to extend into and move; the support 25 is fixed in the second region 22; the operation section controls the connection plate 26 and the moving plate 24 to move relative to the support frame 25, and the water bath liquid moves the upper surface of the moving plate 24 in the first region 21 and the second region 22. The upper end of the moving plate 24 is provided with an electric heating wire 27 to ensure the temperature of the water bath liquid.
Slotted holes are arranged on the upper part and the lower part of the partition baffle in a penetrating way, so that the space at the upper end of the moving plate 24 in the second area 22 is communicated with the first area 21; when the water level line of the water bath liquid descends, the moving plate 24 moves upwards, and the water bath liquid moves upwards through the slotted hole to ensure the water level line; when the water level of the bath solution is too high, the moving plate 24 moves down, and the bath solution moves up through the slot.
A first connecting block 28 is arranged above the separation baffle 23, and a second connecting block 29 matched with the separation baffle is also arranged at the lower end of the incubation device body 1; the first connecting block 28 and the second connecting block 29 are used for stably fixing the incubation device body 1 in the first area 21, so that the device body is prevented from moving upwards. The first connecting block 28 is arranged in an inverted L shape, a T shape or other shapes with the upper part capable of playing a blocking role, and the second connecting block 29 is matched with the first connecting block 28 in shape.
The operating part comprises a first connecting shaft 210 arranged between the support frames 25 on the two sides and a second connecting shaft 211 arranged between the connecting plates 26 on the two sides, a first rotating wheel 212 is arranged on the outer side of the first connecting shaft 210, a second rotating wheel 213 is arranged at the corresponding position on the outer side of the second connecting shaft 211, and the relative positions of the first rotating wheel 212 and the second rotating wheel 213 are fixed; the circle center of the first connecting shaft 210 does not coincide with the circle center of the first rotating wheel 212, and the circle center of the second connecting shaft 211 does not coincide with the circle center of the second rotating wheel 213; the first connecting shaft 210 is movably connected with the support frame 25, and the second connecting shaft 211 is movably connected with the connecting plate 26; a driving part is arranged on one side or both sides of the first connecting shaft 210; the first connecting shaft 210 and the first rotating wheel 212 are driven to rotate by operating the driving part, the second rotating wheel 213 and the second connecting shaft 211 are driven to rotate by the first rotating wheel 212 and the second rotating wheel 213, and the first rotating wheel 212 and the second rotating wheel 213 are eccentrically arranged relative to the first connecting shaft 210 and the second connecting shaft 211 respectively, so that the first rotating wheel 212 and the second rotating wheel 213 can drive the connecting plate 26 and the moving plate 24 to move up and down when rotating relatively. The first rotating wheel 212 and the second rotating wheel 213 have the same diameter.
Two ends of the first rotating wheel 212 or the second rotating wheel 213 are provided with convex rings which just accommodate the second rotating wheel 213 or the first rotating wheel 212; this arrangement ensures stability when the first rotating wheel 212 and the second rotating wheel 213 rotate with respect to each other. The number of the first rotating wheel 212/the number of the second rotating wheel 213 are 2 respectively and symmetrically arranged at two ends, so that the stability of the first connecting shaft 210 and the second connecting shaft 211 in rotation is ensured.
A first gear 214 is arranged on the outer side of the first connecting shaft 210, a second gear 215 is arranged on the outer side of the second connecting shaft 211, and the first gear 214 and the second gear 215 are meshed with each other; the circle center of the first gear 214 and the circle center of the first rotating wheel 212 are horizontally mapped into a point, and the circle center of the second gear 215 and the circle center of the second rotating wheel 213 are horizontally mapped into a point; the arrangement of the first gear 214 and the second gear 215 effectively achieves a self-locking effect.
The driving part is a rotating handle 216 arranged on one side or two sides of the first connecting shaft 210, and the manual rotating mode is convenient to control. Or the driving part is a motor 217 arranged on one side or both sides of the first connecting shaft 210, and a fixing frame 218 for supporting the motor 217 is arranged outside the motor 217; a protection box 219 playing a protection role is arranged on one side or both sides of the temperature control device 2; the electric control mode is time-saving and labor-saving, and the automation is completed.
The outer surface of the temperature control device 2 is provided with a control piece 220, and the control piece 220 is provided with a temperature adjusting button for controlling the temperature of the water bath, a constant temperature indicator lamp, a heating indicator lamp and a time display screen.
When the brain piece/tissue piece incubator is used, brain pieces/tissue pieces are gently and respectively placed on the incubation net 16 during incubation, the incubation device body 1 covers the box cover in a virtual mode and is placed in the temperature control device 2 which reaches the specified temperature, and incubation is started. The brain slice/tissue slice is frequently taken, the operation part moves the moving plate 24 to move upwards after the water level line of the incubation liquid is lowered, the water level line of the water bath liquid is raised, and the incubation is continued. Simple structure, easily unpick and wash, can change and incubate net 16 after every use, also can use the back carefully to wash with distilled water to soak with distilled water (dripping into a few drops of HCL), can use several times repeatedly, and needn't change and incubate net 16 every time.
The above description of the embodiments is only for the understanding of the present invention. It should be noted that modifications could be made to the invention without departing from the principle of the invention, which would also fall within the scope of the claims of the invention.
Claims (10)
1. A porous brain slice incubation device convenient to use comprises an incubation device body and is characterized in that an incubation chamber and an oxygen passage are arranged in the incubation device body, the oxygen passage is communicated with the incubation chamber, and the oxygen passage is connected with an oxygen supply pipeline; the incubation chamber is internally provided with an incubation plate which is movably arranged in the incubation chamber; the incubation plate is provided with at least one incubation hole, and an incubation net for bearing brain slices/tissue slices is arranged below the incubation hole; arranging a partition piece in the incubation chamber, wherein the partition piece divides the interior of the incubation chamber into different spaces; the temperature control device is arranged outside the incubation device body and still uses the principle of a water bath method.
2. The conveniently used multi-hole brain slice incubating device according to claim 1, wherein a cover plate is arranged at the upper end of the incubating device body; furthermore, the first end of the oxygen passage is arranged at the lower end of the incubation chamber, the second end of the oxygen passage is arranged at the left end or the right end of the incubation chamber, and the oxygen passage and the incubation chamber form a closed-loop square shape.
3. The multi-well brain piece incubation device for convenient use according to claim 1, wherein two or more incubation wells are provided, and a single brain piece/tissue piece is placed in a single incubation well.
4. The multi-hole brain piece incubation device convenient to use of any one of claims 1-3, wherein the partition comprises a first partition plate located in the incubation chamber, a second partition plate located in the oxygen passage, and a support plate arranged on the inner wall of the oxygen passage; the first partition plate and the second partition plate are perpendicular to each other, the first partition plate partitions the interior of the incubation chamber, the second partition plate partitions the oxygen passage, and the incubation liquid is placed in the chamber for placing the brain slice/tissue slice; the incubation plate is provided with a through hole for accommodating the first partition plate; furthermore, a third partition plate is arranged in the area where the brain piece/tissue piece is not planned to be incubated, and covers the incubation hole where the brain piece/tissue piece is not planned to be incubated.
5. The conveniently used multi-hole brain piece incubation device according to any of claims 1-3, wherein a temperature sensing piece is arranged in the oxygen passage and/or the incubation chamber to monitor the temperature of the incubation liquid in real time; the problem that only the temperature of the water bath solution can be monitored when the temperature is controlled by adopting a water bath method at present is solved, and more accurate temperature conditions are provided for the incubation of brain slices/tissue slices.
6. The multi-hole brain piece incubation device convenient to use of claim 1, wherein the temperature control device comprises a first area for accommodating the incubation device body and the water bath solution, and a second area positioned below the first area, and the first area and the second area are separated by a separation baffle; a regulating part for regulating the water level of the water bath liquid in the first area is arranged in the second area; this kind of setting up of water line with adjustment water bath liquid can guarantee to be continuously taking brain piece/tissue piece after, it reduces to incubate indoor incubation liquid, can not satisfy the condition of hatching the requirement, satisfy the water bath demand through the mode that directly improves the water line of current water bath liquid this moment, avoid again to hatching indoor new incubation liquid that adds, temperature environment can change on the one hand, be unfavorable for the hatching of brain piece/tissue piece, on the other hand also can take place the disturbance to the position of brain piece/tissue piece that has placed, also be in order to avoid appearing the water bath water line and be less than the indoor incubation liquid of hatching simultaneously, the condition of slope takes place for the incubator, thereby influence the normal incubation of brain piece/tissue piece.
7. The multi-well brain piece incubation device for convenient use according to claim 6, wherein the adjustment member comprises: the movable plate is positioned in the second area, the support frame supports the movable plate, and the operating parts are arranged on the movable plate and the support frame; the lower ends of the two sides of the movable plate are provided with connecting plates, and the support frame is provided with connecting grooves for the connecting plates to extend into and move; the support frame is fixed in the second area; the operation part controls the connecting plate and the moving plate to move relative to the support frame, and the water bath liquid moves the upper part of the moving plate in the first area and the second area; furthermore, the upper end of the movable plate is provided with an electric heating wire to ensure the temperature of the water bath liquid.
8. The multi-hole brain piece incubation device convenient to use of claim 6, wherein slotted holes are arranged on the upper part and the lower part of the dividing baffle plate in a penetrating way, so that the space at the upper end of the moving plate in the second area is communicated with the first area; when the water level line of the water bath liquid descends, the moving plate moves upwards, the water bath liquid moves upwards through the slotted hole, and the water level line is ensured; when the water line of the water bath liquid is too high, the moving plate moves downwards, and the water bath liquid moves upwards through the slotted hole; furthermore, a first connecting block is arranged above the separation baffle, and a second connecting block matched with the first connecting block is also arranged at the lower end of the incubation device body; the incubation device body is stably fixed in the first area by the aid of the first connecting block and the second connecting block, and the situation that the incubation device body moves upwards is avoided.
9. The conveniently-used porous brain slice incubating device according to claim 7, wherein the operating part comprises a first connecting shaft arranged between the supporting frames at two sides and a second connecting shaft arranged between the connecting frames at two sides, a first rotating wheel is arranged at the outer side of the first connecting shaft, a second rotating wheel is arranged at the corresponding position at the outer side of the second connecting shaft, and the relative positions of the first rotating wheel and the second rotating wheel are fixed; the circle center of the first connecting shaft is not coincident with the circle center of the first rotating wheel, and the circle center of the second connecting shaft is not coincident with the circle center of the second rotating wheel; the first connecting shaft is movably connected with the support frame, and the second connecting shaft is movably connected with the connecting plate; a driving part is arranged on one side or both sides of the first connecting shaft; the first connecting shaft and the first rotating wheel are driven to rotate through the operation driving portion, the first rotating wheel and the second rotating wheel are driven to rotate through rotation, and the first rotating wheel and the second rotating wheel are eccentrically arranged relative to the first connecting shaft and the second connecting shaft respectively, so that the first rotating wheel and the second rotating wheel can drive the connecting plate and the moving plate to move up and down when rotating relatively.
10. The conveniently-used porous brain slice incubating device according to claim 9, wherein a first gear is arranged on the outer side of the first connecting shaft, a second gear is arranged on the outer side of the second connecting shaft, and the first gear and the second gear are meshed with each other; the center of the first gear and the center of the first rotating wheel are horizontally mapped into a point, and the center of the second gear and the center of the second rotating wheel are horizontally mapped into a point; the self-locking effect is effectively realized by the arrangement of the first gear and the second gear.
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| 尹雄章: "表没食子儿茶素对小鼠离体脑片及氧糖剥夺损伤HT-22细胞系的保护作用", 《医药导报》 * |
| 汤莹莹等: "MMP-9参与NOTCH3基因R544C点突变小鼠脑损伤机制研究", 《中国优秀硕士学位论文全文数据库 (医药卫生科技辑)》 * |
| 范思远: "谷红注射液抗脑缺血的药效学与作用机制研究", 《中国博士学位论文全文数据库 (医药卫生科技辑)》 * |
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