CN212713575U - Brain slice incubation groove suitable for electrophysiological experiment - Google Patents
Brain slice incubation groove suitable for electrophysiological experiment Download PDFInfo
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- CN212713575U CN212713575U CN202021171675.XU CN202021171675U CN212713575U CN 212713575 U CN212713575 U CN 212713575U CN 202021171675 U CN202021171675 U CN 202021171675U CN 212713575 U CN212713575 U CN 212713575U
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Abstract
The utility model discloses a brain piece incubation groove suitable for electrophysiological experiment, including holding device and bearing frame, wherein: a hollow cavity is arranged in the container, a bearing frame is movably arranged in the hollow cavity, a bubble chamber is fixedly arranged at the side edge of the bearing frame, the bubble chamber is isolated from the bearing frame by a clapboard, an opening is fixedly arranged below the clapboard, the utility model is arranged by the bubble chamber, can limit the bubbles generated by the external mixed gas conveying device to rise in the bubble chamber and isolate the bubbles generated in the gas filling process, can prevent air bubbles from being retained below the section, reduce vibration caused by air bubble aggregation in the brain slice incubation process, ensure stable brain slice incubation, meanwhile, an opening fixedly arranged below the clapboard of the bubble chamber can be communicated with the whole incubation liquid, the incubation liquid is saturated by bubbles rising from the bottom of the bubble chamber, and provides power for the constant circulation of the incubation liquid, and realizes continuous circulation when the incubation liquid is fully saturated.
Description
Technical Field
The utility model belongs to the technical field of neuroscience, concretely relates to brain piece incubation groove suitable for electrophysiological experiment.
Background
Brain slices are a specimen commonly used in neuroscience research at present, by taking out the brain tissue or spinal cord intact from the animal body, cutting into slices of 200-500 μm in artificial cerebrospinal fluid, which retain the activity and function of part of the nerve tissue, and have the advantage of retaining relatively intact nerve circuits and extracellular environment compared with discrete or cultured cells.
In the neurological experiment, brain and spinal cord slices need to be pre-incubated and stored in an incubation groove before being transferred to a recording device, the incubation device is crucial to the maintenance of good physiological state and survival time of a tissue sample, and mixed gas (95% O) needs to be introduced into incubation liquid in the brain slice incubation process2+5％CO2) The rising bubbles can cause the specimen to vibrate and thereby affect tissue viability.
The existing design in the market is used for solving the problem, and two ideas of reducing the brain slice incubation space to avoid rising bubbles or collecting the bubbles into an air chamber through ventilation of a central cylinder and discharging the bubbles are adopted.
But the former comes at the expense of limited incubation space; the closed cylinder design of the latter has certain influence on the liquid circulation in the incubation device; meanwhile, the ceramic bubbler used in the latter is not convenient for cleaning and maintenance of the equipment because the ceramic bubbler needs to be fixed at the bottom of the cylinder and cannot be taken out.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a brain piece incubation groove suitable for electrophysiological experiment to solve foretell technical problem.
In order to achieve the above object, the utility model provides a following technical scheme: a brain slice incubation groove suitable for electrophysiological experiments comprises a container and a bearing frame, wherein:
a hollow cavity is arranged in the container, and a bearing frame is movably arranged in the hollow cavity;
a bubble chamber is fixedly arranged on the side edge of the bearing frame, the bubble chamber is isolated from the bearing frame through a partition plate, and an opening is fixedly arranged below the partition plate;
the bearing frame comprises a base and a support, and the base is fixedly connected with the support through a reinforcing block;
a plurality of mounting holes penetrating through the bracket are uniformly formed in the bracket, and a gauze is movably arranged on the inner wall of each mounting hole;
the mounting hole is internally detachably provided with a mounting ring through a gauze, and an incubation chamber is fixedly arranged inside the mounting ring.
Preferably, the partition board is designed to be of a U-shaped structure, and the bubble chamber is designed to be of a U-shaped structure.
Preferably, the container is a hollow cylindrical cavity structure with an opening at the upper end, the base is a cylindrical structure, the diameter of the base is the same as that of the support, the support is provided with a U-shaped groove corresponding to the bubble chamber, and the distance between the outer wall of the base and the inner wall of the container is 0.1cm-0.5 cm.
Preferably, a bubble stone is movably arranged in the bubble chamber, and the bubble stone is connected with an external mixed gas conveying device through an air duct.
Preferably, the receptacle height is greater than the carrier height.
The utility model discloses a technological effect and advantage: this brain piece incubation groove suitable for electrophysiological experiment:
1. the bubbles generated by the external mixed gas conveying device can be limited to rise in the bubble chamber through the arrangement of the bubble chamber, the bubbles generated in the air-entrapping process are isolated, the bubbles can be prevented from being retained below the slices, the vibration caused by bubble aggregation in the brain slice incubating process is reduced, the brain slices are stably incubated, meanwhile, the opening fixedly arranged below the partition plate of the bubble chamber can be communicated with the whole incubating liquid, the bubbles rising from the bottom of the bubble chamber saturate the incubating liquid, power is provided for the constant circulation of the incubating liquid, and the continuous circulation is realized while the incubating liquid is fully saturated;
2. the multiple incubation chambers are independently arranged, the limited volume in the incubation groove is fully utilized while the structure is kept stable, the brain slices are conveniently classified and placed, the disturbance is not easy to occur, samples from different sources can be incubated simultaneously, and the problem that the existing brain slice incubator is only provided with one incubation chamber and cannot perform comparative observation tests on two experimental treatment conditions simultaneously is solved
3. Simple structure through the container and bear the setting of frame, only need when using bear the frame put into the container can, convenient dismantlement and change, easily set up and disassemble cleanness.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is an exploded view of the present invention;
fig. 3 is a schematic structural diagram of the bearing frame of the present invention.
In the figure: 1-a container, 2-a bearing frame, 3-a bubble chamber, 4-a clapboard, 5-a bubble stone and 6-an air duct;
11-a hollow cavity;
21-base, 22-support, 23-reinforcing block;
221-mounting hole, 222-gauze, 223-mounting ring, 224-incubation chamber;
41-opening.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to fig. 1 to 3 of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a brain piece incubation groove suitable for electrophysiological experiment as shown in fig. 1-3, including container 1 and bearing frame 2, wherein:
the container 1 is inside to be provided with well cavity 11, just well cavity 11 internalization is provided with bears frame 2, divide into container 1 and bear 2 two parts through hatching the groove, during the use only need will bear frame 2 put into container 1 can, easily set up and disassemble cleanness, wherein hold container 1 is inside well cavity 11 can hold incubation liquid, container 1 can replace with 10cm glass crystallization ware on the market.
The utility model discloses when using, only need hug closely 1 inner wall of container with 5 top-down of air bubble stone and put into the bubble chamber 3 can, air bubble stone 5 is small-size air bubble stone, long about 12mm, high about 13mm, do not need additionally to fix air bubble stone 5, and the bubble that outside mist conveyor generated can be restricted to rise in air bubble chamber 3, air bubble chamber 3 can keep apart the bubble that the air entrainment in-process produced, can prevent that the bubble from being detained below the section, it rocks to reduce the liquid level that the bubble broke and produces, make stable hatching of brain piece, opening 41 of the fixed setting in 4 below of 3 baffles in air bubble chamber simultaneously can with whole hatching liquid intercommunication, and make the hatching liquid saturation from the bubble that rises in 3 bottoms in air bubble chamber, and the constancy of liquid provides power.
In the present invention, the reinforcing block 23 can leave a gap between the base 21 and the support 22, so that the bottom surface of the incubation chamber 224 has a sufficient gap, thereby ensuring that the oxygen-saturated incubation liquid can freely circulate in the incubation chamber 224.
Evenly be provided with a plurality of mounting holes 221 that run through support 22 on the support 22, just the activity of mounting hole 221 inner wall is provided with gauze 222 the utility model discloses well gauze 222 adopts the nylon materials setting.
The inside detachable mounting ring 223 that is provided with of gauze 222 of mounting hole 221, just the inside fixed room 224 that incubates that is provided with of mounting ring 223 the utility model discloses well room 224 of incubating sets up to 6, and the mounting ring 223 can set up or remove as required, and the classification of the different experimental samples of being convenient for is placed.
Only one brain slice is placed in each incubation chamber 224, and when one brain slice is selected, other adjacent brain slices are not disturbed, and neurons and glial cells in the brain slices are not stimulated to influence the activity and function of the neurons.
The utility model discloses can set up the collar 223 according to the experiment needs, put gauze 222 on mounting hole 221, then push down collar 223, can make collar 223 chucking, the reuse scissors with unnecessary gauze 222 pruning can.
Specifically, the partition plate 4 is designed to be of a U-shaped structure, and the bubble chamber 3 is designed to be of a U-shaped structure.
Specifically, the container 1 is a hollow cylindrical cavity structure with an open upper end, the base 21 is a cylindrical structure, the diameters of the base 21 and the support 22 are the same, the support 22 is provided with a U-shaped groove corresponding to the bubble chamber 3, the distance between the outer wall of the base 21 and the inner wall of the container 1 is 0.1cm-0.5cm, and the bearing frame 2 is convenient to place.
Specifically, the height of the container 1 is greater than that of the bearing frame 2, so that the brain slices can be fully soaked by the incubation liquid, and the activity of the brain slices can be kept.
The working principle is as follows: the brain slice incubation groove suitable for the electrophysiological experiment has the specific operation steps that:
the method comprises the following steps: the gauze 222 and the mounting ring 223 are arranged on the bracket 22;
step two: placing the bearing frame 2 into the container 1;
step three: adding the incubation liquid required by the experiment into the container 1, and enabling the liquid level of the incubation liquid to be submerged through the gauze 2223mm-5 mm;
step four: the air stone 5 connected with the air supply device is tightly attached to the inner wall of the container 1 from top to bottom and is placed in the air bubble chamber 3, and the air volume of the air supply device is adjusted to enable air bubbles to uniformly overflow upwards;
step five: according to the temperature requirement of the experiment, the whole incubation tank can be placed into a small water bath for heating, and the material drawing operation can be started after the incubation liquid is saturated and the temperature is constant;
step six: after cutting off the brain tissue required by the experiment, gently sucking the brain tissue slice by using a suction pipe and placing the brain tissue slice on the gauze 222, flattening the brain tissue slice by using a small-size writing brush to enable the brain tissue slice to be attached to the gauze 222, and avoiding the brain tissue from being exposed in the air during the period;
when the brain slices need to be taken, the needed brain slices can be sucked out by a suction pipe, and the brain tissues can keep the activity for 6-12 hours in the incubation groove;
step seven: after the experiment, the air stones 5 are taken out and cleaned by alcohol or purified water, the rest tissues and the incubation liquid are discarded, the bearing frame 2 is taken out of the container 1 and cleaned by purified water, and the gauze 222 is taken down and replaced.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (5)
1. The utility model provides a brain piece incubation groove suitable for electrophysiological experiment, includes that splendid attire ware (1) and bear frame (2), its characterized in that:
a hollow cavity (11) is arranged in the container (1), and a bearing frame (2) is movably arranged in the hollow cavity (11);
a bubble chamber (3) is fixedly arranged on the side edge of the bearing frame (2), the bubble chamber (3) is isolated from the bearing frame (2) through a partition plate (4), and an opening (41) is fixedly arranged below the partition plate (4);
the bearing frame (2) comprises a base (21) and a support (22), and the base (21) is fixedly connected with the support (22) through a reinforcing block (23);
a plurality of mounting holes (221) penetrating through the support (22) are uniformly formed in the support (22), and a gauze (222) is movably arranged on the inner wall of each mounting hole (221);
the mounting hole (221) is internally detachably provided with a mounting ring (223) through a gauze (222), and the mounting ring (223) is internally and fixedly provided with an incubation chamber (224).
2. The brain slice incubation groove suitable for electrophysiological experiments, according to claim 1, wherein: the partition plate (4) is designed to be of a U-shaped structure, and the bubble chamber (3) is designed to be of a U-shaped structure.
3. The brain slice incubation groove suitable for electrophysiological experiments, according to claim 1, wherein: the container (1) is of a hollow cylindrical cavity structure with an opening at the upper end, and the base (21) is of a cylindrical structure;
the base (21) and the support (22) have the same diameter, and the support (22) is provided with a U-shaped groove corresponding to the bubble chamber (3);
the distance between the outer wall of the base (21) and the inner wall of the container (1) is 0.1cm-0.5 cm.
4. The brain slice incubation groove suitable for electrophysiological experiments, according to claim 1, wherein: and a bubble stone (5) is movably arranged in the bubble chamber (3), and the bubble stone (5) is connected with an external mixed gas conveying device through an air duct (6).
5. The brain slice incubation groove suitable for electrophysiological experiments, according to claim 1, wherein: the height of the container (1) is greater than that of the bearing frame (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021171675.XU CN212713575U (en) | 2020-06-22 | 2020-06-22 | Brain slice incubation groove suitable for electrophysiological experiment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021171675.XU CN212713575U (en) | 2020-06-22 | 2020-06-22 | Brain slice incubation groove suitable for electrophysiological experiment |
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| Publication Number | Publication Date |
|---|---|
| CN212713575U true CN212713575U (en) | 2021-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021171675.XU Active CN212713575U (en) | 2020-06-22 | 2020-06-22 | Brain slice incubation groove suitable for electrophysiological experiment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
-
2020
- 2020-06-22 CN CN202021171675.XU patent/CN212713575U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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