CN218674755U

CN218674755U - In vivo tissue incubation perfusion imaging recording device

Info

Publication number: CN218674755U
Application number: CN202222969433.0U
Authority: CN
Inventors: 欧阳雨林; 詹阳
Original assignee: Shenzhen Institute of Advanced Technology of CAS; Shenzhen Technology University
Current assignee: Shenzhen Institute of Advanced Technology of CAS; Shenzhen Technology University
Priority date: 2022-11-08
Filing date: 2022-11-08
Publication date: 2023-03-21
Anticipated expiration: 2032-11-08

Abstract

The utility model discloses a perfusion formation of image recorder is hatched to living tissue, this perfusion formation of image recorder is hatched to living tissue is including hatching the container, formation of image record spare, the tissue support plate, handle and pressure net, it prescribes a limit to the holding tank to hatch the container, be equipped with bearing structure on the lateral wall of holding tank, formation of image record spare is located the holding tank and supports on bearing structure, be equipped with mounting hole and fluid hole on the formation of image record spare, the tissue support plate is installed in the mounting hole, be equipped with a plurality of accommodation area that hold the living tissue on the tissue support plate, the handle is connected on formation of image record spare, and stretch out the holding tank setting, pressure net is used for pressing the living tissue on the tissue support plate. This perfusion formation of image recorder is hatched to living body tissue need not centre gripping living body tissue in order to realize the transfer of living body tissue in the use, reduces the damage to the living body tissue, promotes the accuracy of formation of image result to can place a plurality of living body tissues simultaneously in an experiment and image, promote experimental efficiency.

Description

In vivo tissue incubation perfusion imaging recording device

Technical Field

The utility model relates to a biological assay equipment technical field especially relates to a perfusion formation of image recorder is hatched to living tissue.

Background

The brain is the most complex organ of the human body, and the research method thereof is continuously updated and advanced. Direct observation of the brain in vivo is the most desirable method, but because of the tissue imaging depth and the limitations of real-time drug delivery in brain regions, in vivo brain slice experiments become the preferred alternative to observe cell dynamics, signal transmission, and detect the response of living cells to stimuli. At present, a double/multi-photon microscope adopts near-infrared ultrafast laser as an excitation light source, has small cytotoxicity, has deeper penetration to tissue samples, and is particularly suitable for real-time imaging of cranial nerve cells, glial cells and the like in living bodies and living brain slices in the field of neuroscience, so that the double-photon microscope and perfusion equipment are combined together to cooperate with a perfusion imaging recording groove to observe cell activity and signal distribution in experiments.

At present, the imaging groove and the incubation system which are commercialized in the market and matched with the experiment are separated, and only one sample can be placed, in the experimental process, the living brain slice is required to be firstly sucked from the incubation groove and transferred to the self-made imaging groove, and the moving process stimulates the living brain slice, so that the experimental result and efficiency are influenced. The current living brain slice experiment process is as follows: cutting a live brain slice, incubating in an incubation groove, transferring the live brain slice in the incubation groove to an imaging recording groove connected with a perfusion system, and placing the imaging recording groove into an imaging platform for imaging recording.

The method has the following defects that 1, the living brain slice is easy to cause stimulation and damage in the transfer process of an incubation groove and an imaging recording groove, thereby causing abnormal cell tissue state, tissue inflammation and the like; 2. only one sample can be placed in the imaging groove every time, and the time is consumed for replacing the sample after imaging is finished, so that the experiment time is long.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a perfusion formation of image recorder is hatched to living tissue, this perfusion formation of image recorder is hatched to living tissue does not need the transfer of centre gripping living tissue in order to realize the living tissue in the use, reduces the damage to the living tissue, promotes the accuracy of formation of image result to can place a plurality of living tissues simultaneously in an experiment and form images, promote experimental efficiency.

For realizing the above technical effect, the technical scheme of the utility model as follows:

the utility model discloses a perfusion formation of image recorder is hatched to living tissue, include: the incubation container defines an accommodating groove, a supporting structure is arranged on the side wall of the accommodating groove, and incubation liquid is contained in the accommodating groove; the imaging recording part is positioned in the accommodating groove and supported on the supporting structure, a mounting hole and a fluid hole are formed in the imaging recording part, and the mounting hole is communicated with external perfusion equipment; the tissue carrier plate is arranged in the mounting hole, the upper surface of the tissue carrier plate and the upper surface of the imaging recording member are arranged at intervals, the tissue carrier plate is provided with a plurality of accommodating areas which are arranged at intervals, and each accommodating area accommodates a living tissue; the lifting handle is connected to the imaging recording piece and extends out of the accommodating groove; a pressing net for pressing the living tissue on the tissue carrier plate.

In some embodiments, the supporting structure is a plate frame, and the plate frame is disposed on two opposite side walls of the accommodating groove.

In some specific embodiments, the plate frame includes a vertical portion connected to the side wall of the receiving groove, and a horizontal portion connected to one end of the vertical portion and supporting the image recording member.

In some embodiments, the mounting hole comprises a first hole and a second hole, the first hole is located above the second hole, the size of the first hole is smaller than that of the second hole, and the tissue carrier is adhered to the step surfaces of the first hole and the second hole.

In some specific embodiments, the cross-sectional area of the first aperture gradually decreases in a direction toward the second aperture.

In some embodiments, the pressing net comprises a frame and a plurality of pressing wires, and the plurality of pressing wires are arranged at intervals along the length direction or the width direction of the frame.

In some embodiments, the fluid holes are plural, and the plural fluid holes are arranged in plural rows and plural columns.

In some embodiments, the tissue carrier plate is provided with medical silica gel strips arranged at intervals along the length direction and the width direction of the tissue carrier plate, and the plurality of medical silica gel strips divide the surface of the tissue carrier plate into a plurality of accommodating areas.

In some embodiments, the living tissue incubation perfusion imaging recording device further comprises an air supplement device for supplementing gas required for living of the living tissue into the incubation container.

In some embodiments, the handles are two and are respectively connected to two opposite side walls of the imaging recording member.

The utility model discloses a perfusion formation of image recorder is hatched to live body tissue's beneficial effect: because the formation of image record piece passes through bearing structure and supports in incubating the container, and can put into incubating the container or take out from incubating the container through the handle, in actual work process, do not have the step of direct transfer live body tissue again except that put the live body tissue on the tissue support plate at first, reduce the damage to the live body tissue, promote the accuracy of formation of image result, and because have a plurality of accommodation area on the tissue support plate, a live body tissue can all be placed to every accommodation area, realized placing a plurality of live body tissues simultaneously and carried out the function of formation of image, promoted experimental efficiency.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

Fig. 1 is a schematic structural diagram of a living tissue incubation perfusion imaging recording device according to an embodiment of the present invention;

fig. 2 is a sectional view of the frame of the living tissue incubation perfusion imaging recorder according to the embodiment of the present invention;

fig. 3 is a schematic diagram of the matching structure of the imaging recording member and the tissue support plate of the living tissue incubation perfusion imaging recording device according to the embodiment of the present invention;

fig. 4 is a schematic structural diagram of a tissue support plate of a living tissue incubation perfusion imaging recording device according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of a pressing net of the living tissue incubation perfusion imaging recording device according to an embodiment of the present invention.

Reference numerals are as follows:

1. incubating the container;

2. an imaging recording member; 21. mounting holes; 211. a first hole; 212. a second hole; 22. a fluid aperture; 23. hanging holes;

3. organizing a carrier plate; 31. a housing area; 32. medical silica gel strips;

4. a handle;

5. pressing the net; 51. a frame; 52. pressing the filaments;

6. a plate frame; 61. a vertical portion; 62. a horizontal portion;

7. an air supply device.

Detailed Description

In order to make the technical problems, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further explained below by means of specific embodiments in conjunction with the accompanying drawings.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and for simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Furthermore, features defined as "first" and "second" may explicitly or implicitly include one or more of the features for distinguishing between descriptive features, non-sequential, non-trivial and non-trivial. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The specific structure of the living tissue incubation perfusion imaging recording device according to the embodiment of the present invention is described below with reference to fig. 1 to 5.

The utility model discloses a perfusion formation of image recorder is hatched to living tissue, as shown in fig. 1, the perfusion formation of image recorder is hatched to living tissue is including hatching container 1, formation of image record spare 2, tissue support plate 3, handle 4 and pressure net 5, it prescribes a limit to the holding tank to hatch container 1, be equipped with bearing structure on the lateral wall of holding tank, the splendid attire has hatching liquid in the holding tank, formation of image record spare 2 is located the holding tank and supports on bearing structure, be equipped with mounting hole 21 and fluid hole 22 on the formation of image record spare 2, mounting hole 21 and outside perfusion equipment intercommunication, tissue support plate 3 is installed in mounting hole 21, the upper surface of tissue support plate 3 sets up with the upper surface interval of formation of image record spare 2, be equipped with the regional 31 that holds that a plurality of partitions set up on the tissue support plate 3, a living tissue has been held in every holds regional 31, handle 4 is connected on formation of image record spare 2, and stretch out the holding tank setting, pressure net 5 is used for pressing living tissue on tissue 3.

It can be understood that, in the actual working process, a plurality of biopsy tissues which are sliced are respectively placed in a plurality of containing areas 31, then the pressing net 5 is pressed on the biopsy tissues, then the tissue support plate 3 loaded with the biopsy tissues is installed in the installation hole 21, after the placement of the biopsy tissues is completed, a liquid inlet pipe and a liquid outlet pipe which are arranged in an external perfusion device are connected to the side wall of the installation hole 21, the liquid inlet pipe and the liquid outlet pipe cannot touch the biopsy tissues, then the whole imaging recording piece 2 is placed on a supporting structure in the containing groove by the handle 4, so that the liquid in the containing groove can sink the biopsy tissues, after a period of incubation, the imaging recording piece 2 is taken out of the containing groove, the liquid pipeline of the external perfusion device is removed, and the imaging recording piece 2 is placed under an external imaging device for imaging. Therefore, in the using process, the step of directly transferring the movable body tissue is not needed except for initially placing the living body tissue on the tissue carrier plate 3, the damage to the living body tissue is reduced, the accuracy of the imaging result is improved, and because the tissue carrier plate 3 is provided with the plurality of accommodating areas 31, each accommodating area 31 can be used for placing one living body tissue, the function of simultaneously placing a plurality of living body tissues for imaging is realized, and the experimental efficiency is improved.

Preferably, the incubation container 1 is an acrylic box with an open upper end.

In some embodiments, as shown in fig. 2, the supporting structure is a plate frame 6, and the plate frame 6 is disposed on two opposite side walls of the receiving groove. From this, can change grillage 6 according to the size of a dimension of formation of image record 2 for the living body tissue incubation perfusion imaging recording device of this embodiment can use the formation of image record 2 of multiple size, thereby adjust the size of tissue carrier plate 3 according to actual need, in order to realize adjusting the number of the living body tissue of once only formation of image according to actual need, has promoted the user of living body tissue incubation perfusion imaging recording device and has used the satisfaction.

In some specific embodiments, as shown in fig. 2, the rack 6 includes a vertical portion 61 and a horizontal portion 62, the vertical portion 61 being connected to a side wall of the accommodating groove of the incubation container 1, and the horizontal portion 62 being connected to one end of the vertical portion 61 and supporting the image-forming recording member 2. Thereby, the connection stability of the plate frame 6 and the accommodating groove side wall can be ensured, and the plate frame 6 can be ensured to stably support the image forming recording member 2.

In some embodiments, as shown in fig. 3, the mounting hole 21 includes a first hole 211 and a second hole 212, the first hole 211 is located above the second hole 212, and the size of the first hole 211 is smaller than that of the second hole 212, and the tissue carrier 3 is adhered on the stepped surfaces of the first hole 211 and the second hole 212. Therefore, in the actual installation process, the tissue carrier plate 3 can be directly bonded on the step surface from the lower part, and the installation is very convenient.

In some specific embodiments, as shown in FIG. 3, the cross-sectional area of the first aperture 211 gradually decreases in a direction closer to the second aperture 212. It is understood that in practical operation, the liquid conduit of the external perfusion device is connected to the side wall of the first hole 211, and the first hole 211 is formed as a tapered hole to facilitate perfusion.

In some embodiments, as shown in fig. 5, the mesh press 5 includes a frame 51 and a plurality of press wires 52, and the plurality of press wires 52 are arranged at intervals along a length direction or a width direction of the frame 51. Thereby, the pressing wire 52 can press the living tissue against the tissue carrier plate 3, avoiding the living tissue from rising under the action of the buoyancy of the liquid during the incubation process.

In some embodiments, the fluid holes 22 are plural, and the plural fluid holes 22 are arranged in plural rows and plural columns. Thereby, the flow of the incubation liquid in the incubation container 1 is facilitated, and the stable incubation of the living tissue is ensured

In some embodiments, as shown in fig. 4, the tissue carrier plate 3 is provided with medical silicone strips 32 arranged at intervals along the length direction and the width direction thereof, and the plurality of medical silicone strips 32 divide the surface of the tissue carrier plate 3 into a plurality of accommodating areas 31. Therefore, in the process of ensuring that the tissue carrier plate 3 can contain a plurality of living tissues, the living tissues are prevented from interfering with each other.

In some embodiments, the living tissue incubation perfusion imaging recording device further comprises an air supplement device 7, the air supplement device 7 being adapted to supplement the gas required for living tissue survival towards the inside of the incubation container 1. This ensures that living tissue survives during incubation. Preferably, the air supply device 7 is an oxygen-increasing atomized air disc connected with an air bottle.

In some embodiments, the two handles 4 are connected to the two opposite side walls of the image recording member 2. Thereby, the stability when the user lifts up or down the imaging recording member 2 can be improved.

Example (b):

a living tissue incubation perfusion imaging recording device according to an embodiment of the present invention is described below with reference to fig. 1 to 5.

The living tissue incubation perfusion imaging recording device comprises an incubation container 1, an imaging recording piece 2, a tissue carrier plate 3, a lifting handle 4 and a pressing net 5, wherein the incubation container 1 defines a holding tank, and the length, the width and the height of the incubation container 1 are all 80mm. Be equipped with bearing structure on the lateral wall of holding tank, the splendid attire has incubation liquid in the holding tank, and bearing structure is grillage 6, and grillage 6 is established on two relative lateral walls that set up of holding tank. The plate frame 6 includes a vertical portion 61 and a horizontal portion 62, the vertical portion 61 is connected to the accommodating groove side wall, and the horizontal portion 62 is connected to one end of the vertical portion 61 and is used for supporting the image forming recording member 2. The length of the plate frame 6 is 50mm, and the widths of the vertical part 61 and the horizontal part 62 are both 10mm. The image-recording member 2 is located in the accommodating groove and supported on the horizontal portion 62,

the imaging recording member 2 is provided with a mounting hole 21, a fluid hole 22 and four hanging holes 23, and the length and the width of the imaging recording member 2 are both 70mm, and the thickness is 5mm. The aperture of the fluid hole 22 is 0.25mm, and is arranged in multiple rows and multiple columns, the mounting hole 21 is located in the middle of the imaging recording member 2, the mounting hole 21 includes a first hole 211 and a second hole 212, the first hole 211 is located above the second hole 212, the first hole 211 is a tapered hole, the maximum size of the first hole 211 is 45mm × 45mm, the minimum size is 40mm × 40mm, and the size of the second hole 212 is 45mm × 45mm. The tissue carrier plate 3 is a glass plate and is bonded to the step surfaces of the first hole 211 and the second hole 212. The tissue support plate 3 is divided into four separated containing areas 31 through medical silica gel with the thickness of 0.1mm, each containing area 31 contains a living tissue, the number of the handles 4 is two, each handle 4 is provided with two hooks, the hooks are matched with the hanging holes 23, the pressing net 5 comprises a frame 51 and a plurality of pressing wires 52, and the pressing wires 52 are arranged at intervals along the length direction or the width direction of the frame 51. The gas supplementing device 7 protrudes inside the housing groove and is used to supplement the gas required for the survival of the living tissue toward the inside of the incubation container 1.

The using flow of the living tissue incubation perfusion imaging recording device of the embodiment is as follows:

placing the imaging record 2 with the tissue carrier plate 3 mounted thereon into the incubation container 1 using the handle 4; specifically, the tissue carrier plate 3 is bonded to the step faces of the first hole 211 and the second hole 212, and then the imaging recording member 2 is lifted up using the handle 4, placed in the incubation container 1, and stopped against the plate frame 6.

Placing a plurality of living tissues into a plurality of placing accommodation areas 31 of the tissue carrier plate 3;

after incubating for a specified time, pressing the pressing net on the living tissue;

the image recording member 2 is taken out and put under an external image forming apparatus for image recording using the handle 4.

In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The above description is only for the preferred embodiment of the present invention, and for those skilled in the art, there are variations on the detailed description and the application scope according to the idea of the present invention, and the content of the description should not be construed as a limitation to the present invention.

Claims

1. A living tissue incubation perfusion imaging recording device, comprising:

the incubation container (1) defines an accommodating groove, a supporting structure is arranged on the side wall of the accommodating groove, and incubation liquid is contained in the accommodating groove;

the imaging recording piece (2) is positioned in the accommodating groove and supported on the supporting structure, a mounting hole (21) and a fluid hole (22) are formed in the imaging recording piece (2), and the mounting hole (21) is communicated with external perfusion equipment;

the tissue carrier plate (3), the tissue carrier plate (3) is installed in the installation hole (21), the upper surface of the tissue carrier plate (3) and the upper surface of the imaging recording member (2) are arranged at intervals, a plurality of accommodating areas (31) which are arranged at intervals are arranged on the tissue carrier plate (3), and a living tissue is accommodated in each accommodating area (31);

the lifting handle (4) is connected to the imaging recording piece (2) and extends out of the accommodating groove;

a pressing net (5), wherein the pressing net (5) is used for pressing the living tissue on the tissue carrier plate (3).

2. A living tissue incubation perfusion imaging recording device according to claim 1, wherein the supporting structure is a plate frame (6), and the plate frame (6) is arranged on two side walls of the accommodating groove which are oppositely arranged.

3. A living tissue incubation perfusion imaging recording device according to claim 2, characterized in that the ledge (6) comprises a vertical portion (61) and a horizontal portion (62), the vertical portion (61) being connected to the side wall of the receiving groove, the horizontal portion (62) being connected at one end of the vertical portion (61) and being adapted to support the imaging recording member (2).

4. A living tissue incubation perfusion imaging recording device according to any one of claims 1-3, wherein the mounting hole (21) comprises a first hole (211) and a second hole (212), the first hole (211) is located above the second hole (212), the size of the first hole (211) is smaller than that of the second hole (212), and the tissue carrier plate (3) is adhered on the stepped surface of the first hole (211) and the second hole (212).

5. Living tissue incubation perfusion imaging recording device according to claim 4, wherein the cross-sectional area of the first aperture (211) decreases gradually in a direction close to the second aperture (212).

6. Living tissue incubation perfusion imaging recording device according to any one of claims 1-3, wherein the pressing net (5) comprises a frame (51) and a plurality of pressing wires (52), the plurality of pressing wires (52) are arranged at intervals along the length direction or the width direction of the frame (51).

7. The living tissue incubation perfusion imaging recording device according to any one of claims 1-3, wherein the fluid apertures (22) are plural, and the plural fluid apertures (22) are arranged in plural rows and plural columns.

8. A living tissue incubation perfusion imaging recording device according to any one of claims 1-3, characterized in that medical silica gel strips (32) are arranged on the tissue carrier plate (3) at intervals along the length direction and the width direction of the tissue carrier plate, and the plurality of medical silica gel strips (32) divide the surface of the tissue carrier plate (3) into a plurality of accommodating areas (31).

9. Living tissue incubation perfusion imaging recording device according to any of claims 1-3, characterized in that it further comprises an air supply device (7), the air supply device (7) being adapted to supply a gas required for the survival of the living tissue towards the inside of the incubation container (1).

10. Living tissue incubation perfusion imaging recording device according to any one of claims 1-3, wherein the handles (4) are two and are respectively connected to two oppositely arranged side walls of the imaging recording member (2).