CN102812927B - Automatic blood feeder for insect and application method thereof - Google Patents
Automatic blood feeder for insect and application method thereof Download PDFInfo
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- CN102812927B CN102812927B CN201210207401.5A CN201210207401A CN102812927B CN 102812927 B CN102812927 B CN 102812927B CN 201210207401 A CN201210207401 A CN 201210207401A CN 102812927 B CN102812927 B CN 102812927B
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Abstract
The invention relates to an automatic blood sucking device for blood-sucking insects, in particular to an automatic blood feeder for an insect. The blood feeder comprises a constant-temperature blood supply body, an insect collecting funnel, a valve plate, a valve rod and a locking device; the constant-temperature blood supply body comprises a constant-temperature water tank and a constant-temperature plate, wherein the bottom of the constant-temperature water tank is fixedly provided with the constant-temperature plate, the bottom of the constant-temperature plate is provided with a blood groove with a downward opening, and a biological film is fixedly covered on the blood groove; the constant-temperature plate is hermetically located in a conical cavity at the upper part of the insect collecting funnel; the valve plate capable of allowing the insect to come in and go out after being moved upwards is arranged in the insect collecting funnel below the constant-temperature plate; an insect collecting cavity is formed between the bottom surface of the constant-temperature plate and the valve plate; and a plurality of ventilation holes are formed in the valve plate. The blood feeder disclosed by the invention achieves the automatic control of the temperature in the feeding process of the blood-sucking insects, enables the feeding, fixing and releasing of the blood-sucking insects to be more convenient, more efficiency and safer, and provides safety control to researches associated with harmful organisms and diseases of the harmful organisms by the adoption of the reliable and harmless blood resources, thereby having a wider application value.
Description
Technical field
The class insect that the present invention relates to the suck blood device of automatically sucking blood is that a kind of insect is automatically raised blood device.
Background technology
Hematophagus class is the very important medical insect of a class, as tick, mite, flea, lice etc., is communication media or the reservoir of the multiple arthropod-borne diseases such as the plague, Hemorrhagic fever, kala-azar, popular encephalitis B.Therefore, understand, grasp and further investigate this class hematophagus, no matter be in field of biology or all very important at preventive medicine category.Wherein, the life of hematophagus is to be one of important content of media biology and medium prevention and control of diseases with the reproduction of the model of sucking blood.These practical activities be unable to do without hematophagus suck blood observation, the research and analysis of activity, be unable to do without the effective active studies method and apparatus apparatus of sucking blood.With the distinctive host of hematophagus occurring in nature be best as the blood source animal of hematophagus, but but there is inconvenience and difficulty in actual practical activity, the one, the laboratory animal kind of current artificial feeding and breeding is very limited, wherein can be more rare as the animal in hematophagus blood source, in the time of a certain class hematophagus of research, often need to catch a large amount of blood supply animals from occurring in nature, so both need a large amount of human and material resources and the support of financial resources, can not ensure health status, physical signs and the effective supply of blood source animal; It two is in the research of hematophagus transmission, the physiochemical indice of blood supply animal, artificially controlled, Real-Time Monitoring of pathogeny carrying capacity, and this requirement is very difficult in the time being blood supply source with living animal.Therefore, in real hematophagus research process, development and using artificial manufacture to raise blood device be craving for of vast researcher always and wish.But there is no at present can automatically realize temperature control, hematophagus automatically simple and easy to do automatic insect fixing and that discharge raise blood device.
Summary of the invention
The invention provides a kind of insect automatically and raise blood device, overcome the deficiency of above-mentioned prior art, it can effectively solve to there is no at present can realize the problem that temperature control, hematophagus are automatically fixed and discharge automatically.
One of technical scheme of the present invention realizes by following measures: a kind of insect is automatically raised blood device, comprises constant temperature donor, worm collecting bucket, valve plate, valve rod and locking device, constant temperature donor comprises constant temperature water tank and temperature-constant plate, the bottom of constant temperature water tank is fixed with temperature-constant plate, downward opening blood groove is arranged at the bottom of temperature-constant plate, on blood groove, be fixedly coated with biomembrane, temperature-constant plate seal receptacle is in the conical cavity on worm collecting bucket top, in worm collecting bucket below temperature-constant plate, being provided with can be for the valve plate of insect turnover after moving up, between temperature-constant plate bottom surface and valve plate, form collection worm chamber, on valve plate, be distributed with air-vent, the bottom of valve plate is installed with valve rod, the bottom of valve rod is installed with bibcock, the worm collecting bucket lower end of corresponding bibcock is installed with the locking device that can block bibcock, the water inlet pipe and the outlet pipe that communicate with constant temperature water tank inner chamber are arranged at the top of constant temperature water tank, on the temperature-constant plate of blood groove one end, there is the blood receiving hole communicating with blood groove, on the temperature-constant plate of the blood groove other end, there is the blood outlet hole communicating with blood groove, the upper end of blood receiving hole is installed with blood ascending pipe, the upper end of blood outlet hole is installed with transparent blood from overflowing pipe, the top of the top of blood ascending pipe and blood from overflowing pipe lays respectively at the outside of constant temperature water tank, device for detecting temperature is installed on constant temperature water tank.
The further optimization and/or improvements to foregoing invention technical scheme one below:
The bottom of above-mentioned temperature-constant plate can be set with blood film card, blood film card comprises snap ring, interior snap ring and outer snap ring, in the lower inner wall of snap ring, be fixed with interior snap ring, on the outer wall of the bottom of snap ring, be fixed with outer snap ring, wild card ring seal seat is on the top of worm collecting bucket, the internal diameter of snap ring is the 0.5mm to 1.0mm that is greater than temperature-constant plate external diameter, and the internal diameter of interior snap ring is the 2.0mm to 3.0mm that is less than temperature-constant plate external diameter, and the external diameter of outer snap ring is the 2.0mm to 3.0mm that is greater than temperature-constant plate external diameter.
On the bottom outer wall of above-mentioned constant temperature water tank, can have draw-in groove, biomembrane is fixed on draw-in groove by rope.
Above-mentionedly between valve rod and worm collecting bucket, steady rest can be installed, there is the circular hole that can move up and down for valve rod at the center of steady rest.
Above-mentioned blood groove can be bent.
Device for detecting temperature on above-mentioned constant temperature water tank can be thermometer, has thermometer installing hole in the upper end of constant temperature water tank, in thermometer installing hole, is installed with thermometer.
Above-mentioned locking device can comprise lock cylinder, and there is the opening locked groove that can pin bibcock the corresponding both sides, upper end of lock cylinder.
Two of technical scheme of the present invention realizes by following measures: a kind of automatic insect is raised the using method of blood device, carry out in the steps below: the first step, worm collecting bucket is carried out disinfection with the alcohol that percent by volume solution is 65% to 75%, to be that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate bottom through percent by volume solution, then constant temperature donor is held in the conical cavity on worm collecting bucket top, and fix with adhesive tape, prevent from coming off; Second step, by the constant temperature donor being fixedly connected with and worm collecting bucket with temperature-constant plate under mode be inverted on the table, promoting valve rod downwards makes valve plate in open mode, the insect that preprepared is placed in vial is poured in worm collecting bucket, careful vibration, insect is placed in collection worm chamber completely, and upper poppet-valve bar, locks bibcock in the opening locked groove in lock cylinder valve plate is closed; The 3rd step, the worm collecting bucket and the constant temperature donor that add insect are placed on fixed mount in upper mode with temperature-constant plate, fixed mount is can hold the conical section of worm collecting bucket, water pipe connects respectively constant temperature water tank, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer is installed on constant temperature water tank, use syringe in the slow careful injection blood groove of note blood mouth of blood ascending pipe, until can observe the blood face in blood from overflowing pipe preprepared new blood; The 4th step, opens peristaltic pump and supplies water to constant temperature water tank, observes thermometer to till temperature stabilization; The 5th step, raising after blood finishes, rotate bibcock, open valve plate, under worm collecting bucket, place receiving bottle, prepare to receive insect, close peristaltic pump, the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, open the blood in the cooling blood groove of peristaltic pump, make insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 6th step, after insect receives, constant temperature donor and worm collecting bucket are separated, and be placed in ceramic whiteware basin carefully comb choose the insect that is hidden in biomembrane, and all collect, comb has been chosen rear and has been extracted the whole blood in blood out with syringe, pulls down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor and worm collecting bucket are placed in to percent by volume solution.
Three of technical scheme of the present invention realizes by following measures: a kind of automatic insect is raised the using method of blood device, carry out in the steps below: the first step, worm collecting bucket is carried out disinfection with the alcohol that percent by volume solution is 65% to 75%, by being that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate bottom through percent by volume solution, prevent biofilm detachment; Second step, water pipe connects respectively constant temperature water tank, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer is installed on constant temperature water tank, use syringe in the slow careful injection blood groove of note blood mouth of blood ascending pipe, until can observe the blood face in blood from overflowing pipe preprepared new blood; The 3rd step, is placed in constant temperature donor on disc-type bracing frame, constant temperature donor and disc-type bracing frame is placed in to insect and raises in cylinder, opens peristaltic pump and supplies water to constant temperature water tank, observes thermometer to till temperature stabilization; The 4th step, raising after blood finishes, closes peristaltic pump, and the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, opens the blood in the cooling blood groove of peristaltic pump, makes insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 5th step, take out constant temperature donor, constant temperature donor is placed in to ceramic whiteware basin carefully to be combed and chooses the insect that is hidden in biomembrane, and all collect, it is rear with the whole blood in syringe extraction blood that comb has been chosen, pull down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor is placed in to percent by volume solution.
The temperature that the present invention has realized during hematophagus is raised is controlled automatically, make the input, fixing and discharge easier, efficient and safety of hematophagus, and adopt reliable, innoxious blood source, provide safety guarantee to the research of being engaged in pest and disease thereof, there is using value comparatively widely.
Brief description of the drawings
Accompanying drawing 1 is the perspective view of the embodiment of the present invention one.
Accompanying drawing 2 is the perspective view of constant temperature water tank in accompanying drawing 1.
Accompanying drawing 3 is the main TV structure schematic diagram of constant temperature water tank in accompanying drawing 1.
Accompanying drawing 4 is the perspective view of blood film card in accompanying drawing 1.
Accompanying drawing 5 is that the master of temperature-constant plate in accompanying drawing 1 looks structure for amplifying schematic diagram.
What accompanying drawing 6 was temperature-constant plate in accompanying drawing 1 looks up structure for amplifying schematic diagram.
Accompanying drawing 7 is the mounting structure schematic diagram of blood film card and worm collecting bucket in accompanying drawing 1.
Accompanying drawing 8 is the mounting structure schematic diagram of valve body, valve rod and bibcock in accompanying drawing 1.
Accompanying drawing 9 is the structure for amplifying schematic diagram of locking device in accompanying drawing 1.
Accompanying drawing 10 is the structure for amplifying schematic diagram of steady rest in accompanying drawing 1.
Coding in accompanying drawing is respectively: 1 is constant temperature water tank, and 2 is temperature-constant plate, and 3 is blood groove, and 4 is worm collecting bucket, 5 is valve plate, and 6 is collection worm chamber, and 7 is air-vent, and 8 is valve rod, 9 is bibcock, and 10 is lock cylinder, and 11 is water inlet pipe, and 12 is outlet pipe, 13 is blood receiving hole, and 14 is blood outlet hole, and 15 is blood ascending pipe, and 16 is blood from overflowing pipe, 17 is snap ring, and 18 is interior snap ring, and 19 is outer snap ring, and 20 is draw-in groove, 21 is steady rest, and 22 is thermometer, and 23 is thermometer installing hole, and 24 is opening locked groove.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the invention will be further described:
Embodiment mono-: as shown in Fig. 1 to 10, this automatic insect is raised blood device and comprises constant temperature donor, worm collecting bucket, valve plate, valve rod and locking device, constant temperature donor comprises constant temperature water tank 1 and temperature-constant plate 2, the bottom of constant temperature water tank 1 is fixed with temperature-constant plate 2, downward opening blood groove 3 is arranged at the bottom of temperature-constant plate 2, on blood groove 3, be fixedly coated with biomembrane, temperature-constant plate 2 seal receptacles are in the conical cavity on worm collecting bucket 4 tops, in worm collecting bucket 4 below temperature-constant plate 2, being provided with can be for the valve plate 5 of insect turnover after moving up, between temperature-constant plate 2 bottom surfaces and valve plate 5, form collection worm chamber 6, on valve plate 5, be distributed with air-vent 7, the bottom of valve plate 5 is installed with valve rod 8, the bottom of valve rod 8 is installed with bibcock 9, worm collecting bucket 4 lower ends of corresponding bibcock 9 are installed with the locking device that can block bibcock 9, the water inlet pipe 11 and the outlet pipe 12 that communicate with constant temperature water tank 1 inner chamber are arranged at the top of constant temperature water tank 1, on the temperature-constant plate 2 of blood groove 3 one end, there is the blood receiving hole 13 communicating with blood groove 3, on the temperature-constant plate 2 of blood groove 3 other ends, there is the blood outlet hole 14 communicating with blood groove 3, the upper end of blood receiving hole 13 is installed with blood ascending pipe 15, the upper end of blood outlet hole 14 is installed with transparent blood from overflowing pipe 16, the top of the top of blood ascending pipe 15 and blood from overflowing pipe 16 lays respectively at the outside of constant temperature water tank 1, on constant temperature water tank 1, device for detecting temperature is installed.Biomembrane can be also animal skins film, between biomembrane and temperature-constant plate 2 bottoms, should fit tightly, and only leaves the cavity of blood groove 3, and the interior formation gas space of anti-hemostasis groove 3 visionary hope is rung blood supply effect; Blood is entered in blood groove 3 by blood ascending pipe 15, blood receiving hole 13, and can be interior mobile at blood groove 3, blood can be flowed out by blood outlet hole 14, blood from overflowing pipe 16, gas is discharged by blood from overflowing pipe 16 simultaneously, and the interior formation gas space of anti-hemostasis groove 3 visionary hope is rung blood supply effect; The blood supply situation that can also know in blood groove by observing blood from overflowing pipe 16; On the note blood mouth of blood ascending pipe 15, seal rubber plug is installed, facilitates injection syringe needle to insert note blood; Water inlet pipe 11 and outlet pipe 12 are positioned at the top of constant temperature water tank 1, ensure fully flowing of constant temperature water tank 1 interior water body, even water temperature; It is in order to prevent insect escape during raising blood to insect that temperature-constant plate 2 seal receptacles are provided with valve plate 5 in the top of worm collecting bucket 4 and worm collecting bucket 4.
Can according to actual needs, above-mentioned automatic insect be raised to blood device and make further optimization and/or improvements:
As shown in Fig. 1,4,7, the bottom of temperature-constant plate 2 is set with blood film card, blood film card comprises snap ring 17, interior snap ring 18 and outer snap ring 19, in the lower inner wall of snap ring 17, be fixed with interior snap ring 18, on the outer wall of the bottom of snap ring 17, be fixed with outer snap ring 19, outer snap ring 19 envelope seats are on the top of worm collecting bucket 4, the internal diameter of snap ring 17 is the 0.5mm to 1.0mm that is greater than temperature-constant plate 2 external diameters, snap ring 17 can closely be buckled in biomembrane on temperature-constant plate 2, and its function is to prevent blood from overflowing blood groove 3 and prevent from collecting the insect escape in worm chamber 6; The internal diameter of interior snap ring 18 is the 2.0mm to 3.0mm that is less than temperature-constant plate 2 external diameters, and in ensureing, snap ring 18 can make biomembrane closely be buckled on temperature-constant plate 2, and the insect that prevents from collecting in worm chamber 6 escapes; The external diameter of outer snap ring 19 is the 2.0mm to 3.0mm that is greater than temperature-constant plate 2 external diameters, ensures that outer snap ring 19 can fit tightly with the cone inwall on worm collecting bucket 4 tops, prevents the escape of insect.
As shown in Figure 2,3, on the bottom outer wall of constant temperature water tank 1, there is draw-in groove 20, biomembrane is fixed on draw-in groove 20 with rope.In order to be easily installed in dismounting, can biomembrane be fixed on draw-in groove 20 with rope.
As shown in Fig. 1,7,10, in order to strengthen the steadiness of valve rod 8 in worm collecting bucket 4, between valve rod 8 and worm collecting bucket 4, steady rest 21 is installed, there is the circular hole that can move up and down for valve rod 8 at steady rest 21 center.
As shown in Figure 5,6, raise blood area for what increase insect, blood groove 3 is bent.
As shown in Figure 1, 2, the device for detecting temperature on constant temperature water tank 1 is thermometer 22, in order to monitor real-time and easily the temperature of constant temperature water tank 1 interior water, has thermometer installing hole 23 in the upper end of constant temperature water tank 1.
As shown in Figure 9, locking device comprises lock cylinder 10, and there is the opening locked groove 24 that can pin bibcock the corresponding both sides, upper end of lock cylinder 10, by rotating bibcock 9, can realize locking and opening to bibcock 9.
Embodiment bis-: a kind of automatic insect is raised the using method of blood device, carry out in the steps below: the first step, be that 65% to 75% alcohol carries out disinfection to worm collecting bucket 4 with percent by volume solution, to be that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate 2 bottoms through percent by volume solution, then constant temperature donor is held in the conical cavity on worm collecting bucket 4 tops, and fix with adhesive tape, prevent from coming off; Second step, by the constant temperature donor being fixedly connected with and worm collecting bucket 4 with temperature-constant plate 2 under mode be inverted on the table, promoting valve rod 8 downwards makes valve plate 5 in open mode, the insect that preprepared is placed in vial is poured in worm collecting bucket 4, careful vibration, insect is placed in collection worm chamber completely, and upper poppet-valve bar 8, locks bibcock 9 in the opening locked groove 24 in lock cylinder 10 valve plate 5 is closed; The 3rd step, the worm collecting bucket 4 and the constant temperature donor that add insect are placed on fixed mount in upper mode with temperature-constant plate 2, fixed mount is can hold the conical section of worm collecting bucket 4, water pipe connects respectively constant temperature water tank 1, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer 22 is installed on constant temperature water tank 1, use syringe in the slow careful injection blood groove 3 of note blood mouth of blood ascending pipe 15, until can observe the blood face in blood from overflowing pipe 16 preprepared new blood; The 4th step, opens peristaltic pump and supplies water to constant temperature water tank 1, observes thermometer 22 to till temperature stabilization; The 5th step, raising after blood finishes, rotate bibcock 9, open valve plate 5, place receiving bottle 4 times at worm collecting bucket, prepare to receive insect, close peristaltic pump, the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, open the blood in the cooling blood groove 3 of peristaltic pump, make insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 6th step, after insect receives, constant temperature donor and worm collecting bucket 4 are separated, and be placed in ceramic whiteware basin carefully comb choose the insect that is hidden in biomembrane, and all collect, comb has been chosen rear and has been extracted the whole blood in blood groove 3 out with syringe, pulls down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor and worm collecting bucket 4 are placed in to percent by volume solution.In the time that needs are fed to specific objective insect, use the method.
Embodiment tri-: a kind of automatic insect is raised the using method of blood device, carry out in the steps below: the first step, be that 65% to 75% alcohol carries out disinfection to worm collecting bucket 4 with percent by volume solution, by being that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate 2 bottoms through percent by volume solution, prevent biofilm detachment; Second step, water pipe connects respectively constant temperature water tank 1, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer 22 is installed on constant temperature water tank 1, use syringe in the slow careful injection blood groove 3 of note blood mouth of blood ascending pipe 15, until can observe the blood face in blood from overflowing pipe 16 preprepared new blood; The 3rd step, is placed in constant temperature donor on disc-type bracing frame, constant temperature donor and disc-type bracing frame is placed in to insect and raises in cylinder, opens peristaltic pump and supplies water to constant temperature water tank 1, observes thermometer 22 to till temperature stabilization; The 4th step, raising after blood finishes, closes peristaltic pump, and the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, opens the blood in the cooling blood groove 3 of peristaltic pump, makes insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 5th step, take out constant temperature donor, constant temperature donor is placed in to ceramic whiteware basin carefully to be combed and chooses the insect that is hidden in biomembrane, and all collect, it is rear with the whole blood in syringe extraction blood groove 3 that comb has been chosen, pull down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor is placed in to percent by volume solution.The method is fed for insect in enormous quantities, in the time that needs carry out feeding of propagating insects in enormous quantities, only uses constant temperature donor.
Claims (12)
1. an automatic insect is raised the using method of blood device, it is characterized in that carrying out in the steps below: the first step, worm collecting bucket is carried out disinfection with the alcohol that percent by volume solution is 65% to 75%, to be that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate bottom through percent by volume solution, then constant temperature donor is held in the conical cavity on worm collecting bucket top, and fix with adhesive tape, prevent from coming off; Second step, by the constant temperature donor being fixedly connected with and worm collecting bucket with temperature-constant plate under mode be inverted on the table, promoting valve rod downwards makes valve plate in open mode, the insect that preprepared is placed in vial is poured in worm collecting bucket, careful vibration, insect is placed in collection worm chamber completely, and upper poppet-valve bar, locks bibcock in the opening locked groove in lock cylinder valve plate is closed; The 3rd step, the worm collecting bucket and the constant temperature donor that add insect are placed on fixed mount in upper mode with temperature-constant plate, fixed mount is can hold the conical section of worm collecting bucket, water pipe connects respectively constant temperature water tank, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer is installed on constant temperature water tank, use syringe in the slow careful injection blood groove of note blood mouth of blood ascending pipe, until can observe the blood face in blood from overflowing pipe preprepared new blood; The 4th step, opens peristaltic pump and supplies water to constant temperature water tank, observes thermometer to till temperature stabilization; The 5th step, raising after blood finishes, rotate bibcock, open valve plate, under worm collecting bucket, place receiving bottle, prepare to receive insect, close peristaltic pump, the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, open the blood in the cooling blood groove of peristaltic pump, make insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 6th step, after insect receives, constant temperature donor and worm collecting bucket are separated, and be placed in ceramic whiteware basin carefully comb choose the insect that is hidden in biomembrane, and all collect, comb has been chosen rear and has been extracted the whole blood in blood out with syringe, pulls down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor and worm collecting bucket are placed in to percent by volume solution; Wherein:
This automatic insect is raised blood device and comprises constant temperature donor, worm collecting bucket, valve plate, valve rod and locking device, constant temperature donor comprises constant temperature water tank and temperature-constant plate, the bottom of constant temperature water tank is fixed with temperature-constant plate, downward opening blood groove is arranged at the bottom of temperature-constant plate, on blood groove, be fixedly coated with biomembrane, temperature-constant plate seal receptacle is in the conical cavity on worm collecting bucket top, in worm collecting bucket below temperature-constant plate, being provided with can be for the valve plate of insect turnover after moving up, between temperature-constant plate bottom surface and valve plate, form collection worm chamber, on valve plate, be distributed with air-vent, the bottom of valve plate is installed with valve rod, the bottom of valve rod is installed with bibcock, the worm collecting bucket lower end of corresponding bibcock is installed with the locking device that can block bibcock, the water inlet pipe and the outlet pipe that communicate with constant temperature water tank inner chamber are arranged at the top of constant temperature water tank, on the temperature-constant plate of blood groove one end, there is the blood receiving hole communicating with blood groove, on the temperature-constant plate of the blood groove other end, there is the blood outlet hole communicating with blood groove, the upper end of blood receiving hole is installed with blood ascending pipe, the upper end of blood outlet hole is installed with transparent blood from overflowing pipe, the top of the top of blood ascending pipe and blood from overflowing pipe lays respectively at the outside of constant temperature water tank, device for detecting temperature is installed on constant temperature water tank.
2. automatic insect according to claim 1 is raised the using method of blood device, and it is characterized in that has draw-in groove on the bottom outer wall of constant temperature water tank, and biomembrane is fixed on draw-in groove by rope.
3. automatic insect according to claim 1 and 2 is raised the using method of blood device, it is characterized in that, between valve rod and worm collecting bucket, steady rest is installed, and there is the circular hole that can move up and down for valve rod at the center of steady rest.
4. automatic insect according to claim 3 is raised the using method of blood device, it is characterized in that blood groove is bent.
5. automatic insect according to claim 4 is raised the using method of blood device, it is characterized in that the device for detecting temperature on constant temperature water tank is thermometer, has thermometer installing hole in the upper end of constant temperature water tank, in thermometer installing hole, is installed with thermometer.
6. automatic insect according to claim 5 is raised the using method of blood device, it is characterized in that locking device comprises lock cylinder, and there is the opening locked groove that can pin bibcock the corresponding both sides, upper end of lock cylinder.
7. an automatic insect is raised the using method of blood device, it is characterized in that carrying out in the steps below: the first step, worm collecting bucket is carried out disinfection with the alcohol that percent by volume solution is 65% to 75%, by being that the biomembrane that 65% to 75% alcohol disinfecting is crossed is closely fixed on temperature-constant plate bottom through percent by volume solution, prevent biofilm detachment; Second step, water pipe connects respectively constant temperature water tank, peristaltic pump and water bath, the temperature of water in water bath is adjusted to required temperature, thermometer is installed on constant temperature water tank, use syringe in the slow careful injection blood groove of note blood mouth of blood ascending pipe, until can observe the blood face in blood from overflowing pipe preprepared new blood; The 3rd step, is placed in constant temperature donor on disc-type bracing frame, constant temperature donor and disc-type bracing frame is placed in to insect and raises in cylinder, opens peristaltic pump and supplies water to constant temperature water tank, observes thermometer to till temperature stabilization; The 4th step, raising after blood finishes, closes peristaltic pump, and the water pipe that connects water bath one end is placed in to the large beaker that fills frozen water, opens the blood in the cooling blood groove of peristaltic pump, makes insect dissociate and get off to enter receiving bottle from biomembrane, until all receive; The 5th step, take out constant temperature donor, constant temperature donor is placed in to ceramic whiteware basin carefully to be combed and chooses the insect that is hidden in biomembrane, and all collect, it is rear with the whole blood in syringe extraction blood that comb has been chosen, pull down biomembrane, be positioned in clinical waste collection container, it is 65% to 75% alcohol soaking disinfection that constant temperature donor is placed in to percent by volume solution; Wherein:
This automatic insect is raised blood device and comprises constant temperature donor, worm collecting bucket, valve plate, valve rod and locking device, constant temperature donor comprises constant temperature water tank and temperature-constant plate, the bottom of constant temperature water tank is fixed with temperature-constant plate, downward opening blood groove is arranged at the bottom of temperature-constant plate, on blood groove, be fixedly coated with biomembrane, temperature-constant plate seal receptacle is in the conical cavity on worm collecting bucket top, in worm collecting bucket below temperature-constant plate, being provided with can be for the valve plate of insect turnover after moving up, between temperature-constant plate bottom surface and valve plate, form collection worm chamber, on valve plate, be distributed with air-vent, the bottom of valve plate is installed with valve rod, the bottom of valve rod is installed with bibcock, the worm collecting bucket lower end of corresponding bibcock is installed with the locking device that can block bibcock, the water inlet pipe and the outlet pipe that communicate with constant temperature water tank inner chamber are arranged at the top of constant temperature water tank, on the temperature-constant plate of blood groove one end, there is the blood receiving hole communicating with blood groove, on the temperature-constant plate of the blood groove other end, there is the blood outlet hole communicating with blood groove, the upper end of blood receiving hole is installed with blood ascending pipe, the upper end of blood outlet hole is installed with transparent blood from overflowing pipe, the top of the top of blood ascending pipe and blood from overflowing pipe lays respectively at the outside of constant temperature water tank, device for detecting temperature is installed on constant temperature water tank.
8. automatic insect according to claim 7 is raised the using method of blood device, and it is characterized in that has draw-in groove on the bottom outer wall of constant temperature water tank, and biomembrane is fixed on draw-in groove by rope.
9. raise the using method of blood device according to the automatic insect described in claim 7 or 8, it is characterized in that, between valve rod and worm collecting bucket, steady rest is installed, there is the circular hole moving up and down for valve rod at the center of steady rest.
10. automatic insect according to claim 9 is raised the using method of blood device, it is characterized in that blood groove is bent.
11. automatic insects according to claim 10 are raised the using method of blood device, it is characterized in that the device for detecting temperature on constant temperature water tank is thermometer, have thermometer installing hole in the upper end of constant temperature water tank, in thermometer installing hole, are installed with thermometer.
12. automatic insects according to claim 11 are raised the using method of blood device, it is characterized in that locking device comprises lock cylinder, and there is the opening locked groove that can pin bibcock the corresponding both sides, upper end of lock cylinder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210207401.5A CN102812927B (en) | 2012-06-21 | 2012-06-21 | Automatic blood feeder for insect and application method thereof |
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| CN201210207401.5A CN102812927B (en) | 2012-06-21 | 2012-06-21 | Automatic blood feeder for insect and application method thereof |
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| CN102812927B true CN102812927B (en) | 2014-07-16 |
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| CN104719260A (en) * | 2015-03-03 | 2015-06-24 | 常州市疾病预防控制中心 | Water supply device for breeding insects |
| US10888071B2 (en) * | 2016-02-23 | 2021-01-12 | The United States Of America, As Represented By The Secretary Of Agriculture | In vitro parasite feeding system |
| CN105831018B (en) * | 2016-03-31 | 2019-02-26 | 新疆维吾尔自治区疾病预防控制中心 | Insect automatic fine raises blood device |
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| US4850305A (en) * | 1987-12-02 | 1989-07-25 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| EP0396412A1 (en) * | 1989-05-04 | 1990-11-07 | Biological Crop Protection Limited | Pest control |
| US5133289A (en) * | 1991-09-05 | 1992-07-28 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| CN2393336Y (en) * | 1999-08-26 | 2000-08-30 | 中国人民解放军军事医学科学院微生物流行病研究所 | Blood supplier for bloodsuckers |
| EP1492402B1 (en) * | 2002-04-05 | 2008-08-20 | Sanaria Inc. | Apparatuses and methods for the production of haematophagous organisms and parasites suitable for vaccine production |
| CN201797872U (en) * | 2010-09-03 | 2011-04-20 | 朱国鼎 | System for feeding blood to adult mosquitoes manually |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040069235A1 (en) * | 2002-09-27 | 2004-04-15 | Rasa Cordelia G. | Flea feeding apparatus |
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- 2012-06-21 CN CN201210207401.5A patent/CN102812927B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4850305A (en) * | 1987-12-02 | 1989-07-25 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| EP0396412A1 (en) * | 1989-05-04 | 1990-11-07 | Biological Crop Protection Limited | Pest control |
| US5133289A (en) * | 1991-09-05 | 1992-07-28 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
| CN2393336Y (en) * | 1999-08-26 | 2000-08-30 | 中国人民解放军军事医学科学院微生物流行病研究所 | Blood supplier for bloodsuckers |
| EP1492402B1 (en) * | 2002-04-05 | 2008-08-20 | Sanaria Inc. | Apparatuses and methods for the production of haematophagous organisms and parasites suitable for vaccine production |
| CN201797872U (en) * | 2010-09-03 | 2011-04-20 | 朱国鼎 | System for feeding blood to adult mosquitoes manually |
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| CN102812927A (en) | 2012-12-12 |
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