CN105831018B - Insect automatic fine raises blood device - Google Patents
Insect automatic fine raises blood device Download PDFInfo
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- CN105831018B CN105831018B CN201610197846.8A CN201610197846A CN105831018B CN 105831018 B CN105831018 B CN 105831018B CN 201610197846 A CN201610197846 A CN 201610197846A CN 105831018 B CN105831018 B CN 105831018B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The present invention relates to laboratories to raise blood engineering device technique field, it is a kind of insect automatic fine feeding blood device, including raising blood shell, temperature adjustment pipeline, note blood channel and biological Membrane cover, temperature adjustment pipeline and note blood channel are equipped in blood shell raising, the both ends of temperature adjustment pipeline, which are respectively water filling port and discharge outlet and stretch out in, raises outside blood shell, and the partially sealed of temperature adjustment pipeline is enclosed in interior biological Membrane cover raising to have in blood shell;One end of note blood channel, which stretches out in, raises that blood shell is outer and be known as infusing blood mouth, and the other end sealing for infusing blood channel penetrates in biological Membrane cover and referred to as blood outlet.The present invention can form blood supply animal by biological Membrane cover, positioned at the blood sample deposited in haemocoele and suitable temperature simulation, enable hematophagus will be voluntarily by biological Membrane cover gettering site in depositing the blood sample in haemocoele, it can be fully entered in insect receiving bottle after the completion of it is sucked blood, it is easy to operate, it is easy to use, so that it is guaranteed that experimentation go on smoothly and the accuracy of experimental result.
Description
Technical field
The present invention relates to laboratories to raise blood engineering device technique field, is a kind of insect automatic fine feeding blood device.
Background technique
Hematophagus is a kind of very important medical insect, such as tick, mite, flea, lice are the plague, Hemorrhagic fever, black heat
The communication media or reservoir of a variety of arthropod-borne diseases such as disease, popular encephalitis B.Therefore, understand, grasp and further investigate this
Class hematophagus, it is either still all particularly significant in preventive medicine scope in field of biology.Wherein, the life of hematophagus
The reproduction of history and model of sucking blood is one of media biology and the important content of borne diseases prevention and control.These practical activities from
The observation to hematophagus drinking feature, research and analysis are not opened, be unable to do without effective drinking feature research method and are set
Standby instrument.It is best for using the blood source animal of hematophagus distinctive host as hematophagus in nature, but in reality
Practical activity in but there are many difficulties and inconvenience.
The experimental animal type of artificial feeding current first and breeding is extremely limited, wherein can be as the blood of hematophagus
Source animal is even more very rare, generally requires to capture a large amount of blood supply animals from nature when studying certain a kind of hematophagus,
A large amount of human and material resources, financial resources support had both been needed in this way, cannot guarantee that the health status of blood source animal, physical signs and had
Effect supply;Secondly in the research of hematophagus transmission, physiochemical indice, the pathogen capacity of blood supply animal are to need artificially may be used
Control in real time can monitor, and this requirement is very difficult when being blood supply blood source with living animal.Therefore, in reality
In hematophagus research process, development and the made feeding blood device of application are always the craving of vast researcher and wish
It hopes.However mostly to there is structure simple and crude for existing feeding blood device, cannot achieve automatic temperature-controlled, cumbersome, especially suck blood elder brother
The fixation of worm and release process are easily made a fault, thus the problems such as influencing experimental result.
Summary of the invention
The present invention provides a kind of insect automatic fines to raise blood device, overcomes the defects of the prior art, can be effective
It solves existing feeding blood device there are structures simple and crude, cannot achieve automatic temperature-controlled, cumbersome, the especially fixation of hematophagus
It easily makes a fault with release process, thus the problem of influencing experimental result.
The technical scheme is that realized by following measures: a kind of insect automatic fine feeding blood device, including raise
Blood shell, temperature adjustment pipeline, note blood channel and biological Membrane cover are equipped with temperature adjustment pipeline and note blood channel, temperature adjustment pipe raising in blood shell
The both ends in road, which are respectively water filling port and discharge outlet and stretch out in, raises outside blood shell, has raising in blood shell by the office of temperature adjustment pipeline
Portion's sealing is enclosed in interior biological Membrane cover;One end of note blood channel, which stretches out in, to be raised outside blood shell and is known as infusing blood mouth, and blood channel is infused
Other end sealing penetrate in biological Membrane cover and referred to as blood outlet;Raise blood shell and temperature adjustment pipeline intersection and with note blood vessel
The intersection in road is to be tightly connected, and is equipped on raising blood shell and takes chamber in insect and communicate and capable of opening and closing insect inlet and elder brother
Worm outlet, being equipped on raising blood shell can make insect take the ventilation that chamber is connected to outside atmosphere and can prevent hematophagus from escaping in
Sieve pore;Formed on the outside of temperature adjustment pipeline and on the outside of note blood channel and between biological Membrane cover inner cavity and deposit haemocoele, on the outside of temperature adjustment pipeline with
Insect, which is formed, between biological Membrane cover outside and feeding blood case inside on the outside of note blood channel takes chamber in.
Here is the further optimization and/or improvements to invention technology described above scheme:
One of technical solution: above-mentioned feeding blood shell can in a tubular form and there are the opening being mutually communicated in left and right ends, and is raising blood shell
Closure has the top plug of energy plug in the left side opening of body, and the bottom plug for having energy plug is blocked in the right openings for raising blood shell, is pushed up
Plug and bottom plug are elastic plug;From top, plug is pierced by and is located at the outside of top plug for water filling port and note blood mouth, and discharge outlet is from bottom plug
It is pierced by and is located on the outside of bottom plug;It is sleeved on the outside of the middle section of temperature adjustment pipeline positioned at the intracorporal biological Membrane cover of blood shell is raised, and biomembrane
The left end sealing of set is lock on the outside of the right part of note blood channel and on the outside of the temperature adjustment pipeline of corresponding position, the right end of biological Membrane cover
Sealing is lock on the outside of the temperature adjustment pipeline of corresponding position;The side that chamber communicates can be taken in insect by being equipped on the side wall for raising blood shell
Outlet, is equipped with the corking that can be removably mounted, being distributed in corking can make insect take chamber in is connected to outside atmosphere on side outlet
And the ventilation sieve pore that can prevent hematophagus from escaping.
The two of technical solution: above-mentioned feeding blood shell can in a tubular form and there are the opening being mutually communicated in left and right ends, and is raising blood shell
The top plug for having energy plug is blocked in the left side opening of body, top plug is elastic plug;It is equipped in the right openings for raising blood shell
The corking that can be removably mounted, being distributed in corking, which can make insect take chamber in, is connected to outside atmosphere and can prevent hematophagus from escaping
Ventilation sieve pore;From the outside for filling in and being pierced by and being located at top plug is pushed up, temperature adjustment pipeline is in be open to the left for water filling port, discharge outlet and note blood mouth
U-tube shape, and the straight sections to link together with water filling port are known as filling the water section;It is set with positioned at the intracorporal biological Membrane cover of blood shell is raised
On the outside of the middle part of water filling section, and the left end sealing of biological Membrane cover lock onto the right part outside and corresponding position for infusing blood channel
It fills the water on the outside of section, the right end sealing of biological Membrane cover is lock on the outside of the water filling section of corresponding position.
The set that the two can be fettered to limit can be set on the outside of the temperature adjustment pipeline and note blood channel of above-mentioned corresponding top plug position
Pipe, bobbin seal is set in the plug of top, and the both ends of casing are piercing in the left and right sides of top plug respectively, in casing and temperature adjustment pipeline
And sealed colloid is marked in the gap of note blood channel.
Above-mentioned corking may include circle shape lid and gas-permeable nets, encloses in the middle part of shape lid and is equipped with gas-permeable nets, divide on gas-permeable nets
Being furnished with can make insect take the ventilation sieve pore that chamber is connected to outside atmosphere and can prevent hematophagus from escaping in, and gas-permeable nets are fine and closely woven thin,tough silk
Yarn or metallic sieve;Circle shape lid is buckled by end cap and is mounted on one with the right openings of corresponding side outlet or feeding blood shell
It rises.
Above-mentioned biology Membrane cover can be rat-tail biomembrane or artificial membrane;On the outside of the right part of note blood channel and corresponding position
Constraint is set with and the two is closely enclosed in interior rubber ring on the outside of the temperature adjustment pipeline set, and the left end of biological Membrane cover passes through circle shape card
Sealing shroud is bound round on the outside of rubber ring, is set with anti-skid washer between circle shape clip and biological Membrane cover;The right end of biological Membrane cover
Through circle shape clip sealing shroud on the outside of the temperature adjustment pipeline of corresponding position, it is set between circle shape clip and biological Membrane cover anti-
Skidding circle.
It is above-mentioned to may also include temperature automatically controlled water circle device, temperature control component, temperature control are equipped in temperature automatically controlled water circle device
Operation panel is equipped with water inlet and water outlet with water can be pumped out to the circulating pump to water outlet, temperature automatically controlled water circle device
Mouthful, water outlet is connected with water filling port by connecting tube, and water inlet is connected with discharge outlet by connecting tube.
The note blood connector that blood channel links together can be had and infuse by setting on above-mentioned note blood mouth, infused and be equipped with and can pull out in blood connector
Slotting note blood glue plug.
Structure of the invention is reasonable and compact, use the present invention can by biological Membrane cover, positioned at the blood sample deposited in haemocoele
This and suitable temperature simulation form blood supply animal, enable hematophagus will be voluntarily by biology Membrane cover gettering site in depositing haemocoele
Interior blood sample can all exit into insect receiving bottle by changing its local environment after the completion of it is sucked blood
It is interior, it is easy to operate, it is easy to use, so that it is guaranteed that experimentation go on smoothly and the accuracy of experimental result, pass through connection
Temperature automatically controlled water circle device can realize the automatic control of temperature, be further simplified operating process, improve conventional efficient, can also mention
The fidelity of high blood supply animal imitating.
Detailed description of the invention
Attached drawing 1 is the main view broken section schematic diagram of the embodiment of the present invention one.
Attached drawing 2 is the main view broken section schematic diagram of the embodiment of the present invention two.
Coding in attached drawing is respectively as follows: 1 to raise blood shell, and 2 be temperature adjustment pipeline, and 3 be note blood channel, and 4 be biological Membrane cover, and 5
It is discharge outlet for water filling port, 6,7 be note blood mouth, and 8 be blood outlet, and 9 take chamber in deposit haemocoele, 10 for insect, and 11 be top plug, and 12 are
Bottom plug, 13 be corking, and 14 be ventilation sieve pore, and 15 be casing, and 16 be sealed colloid, and 17 buckle for end cap, and 18 be rubber ring, and 19 are
Shape clip is enclosed, 20 be anti-skid washer, and 21 be temperature automatically controlled water circle device, and 22 be water inlet, and 23 be water outlet, and 24 be connection
Pipe, 25 be note blood connector, and 26 be note blood glue plug.
Specific embodiment
The present invention is not limited by the following examples, can determine according to the technique and scheme of the present invention with actual conditions specific
Embodiment.
In the present invention, for ease of description, the description of the relative positional relationship of each component is according to Figure of description 1
Butut mode be described, such as: front, rear, top, and bottom, left and right positional relationship be the Butut according to Figure of description
Direction determines.
Below with reference to examples and drawings, the invention will be further described:
As shown in attached drawing 1,2, it includes raising blood shell 1, temperature adjustment pipeline 2, note 3 and of blood channel which, which raises blood device,
Biological Membrane cover 4 is equipped with temperature adjustment pipeline 2 and note blood channel 3 raising in blood shell 1, the both ends of temperature adjustment pipeline 2 are respectively water filling port 5
With discharge outlet 6 and stretch out in raise blood shell 1 outside, raise blood shell 1 in have by temperature adjustment pipeline 2 it is partially sealed be enclosed in
Biological Membrane cover 4;One end of note blood channel 3, which stretches out in, raises outside blood shell 1 and is known as infusing blood mouth 7, the other end sealing of note blood channel 3
It penetrates in biological Membrane cover 4 and referred to as blood outlet 8;Raise the intersection of blood shell 1 and temperature adjustment pipeline 2 and the friendship with note blood channel 3
It is to be tightly connected at boundary, is equipped on raising blood shell 1 and takes chamber 10 in insect and communicate and capable of opening and closing insect inlet and insect
Outlet, being equipped on raising blood shell 1, which can make insect take chamber 10 in, is connected to outside atmosphere and can prevent changing for hematophagus escape
Air sifter hole 14;It is formed between 2 outside of temperature adjustment pipeline and 3 outside of note blood channel and 4 inner cavity of biological Membrane cover and deposits haemocoele 9, temperature adjustment pipe
Insect, which is formed, between 1 inside of on the outside of road 2 and 3 outside of note blood channel and 4 outside of biological Membrane cover and feeding blood shell takes chamber 10 in.Root
According to demand, insect inlet and insect outlet can be that perhaps insect inlet and ventilation sieve pore 14 are same place or insect to same place
Outlet is that perhaps three is same place to same place or three is respectively different location with ventilation sieve pore 14.When in use, may be used
The constant temperature water supply equipment at access energy sustainable supply constant temperature water source on water filling port 5, and discharge outlet 6 is connect in Sewage treatment container
On;First insect inlet is opened, and has the opening of the insect container of hematophagus to be tested to be directed at insect inlet storage, is passed through
Slight jitter, which fully enters all hematophaguses after insect is taken in chamber 10, can be completed hematophagus for insect inlet closing
Injection process;Then note blood instrument connection note blood mouth 7 can be injected blood sample to depositing in haemocoele 9, when depositing the blood in haemocoele 9
Liquid stops note blood operation after being full of, block note blood mouth 7 after note blood instrument is removed and complete note blood process;Again by constant temperature water supply
The supply water temperature of equipment is adjusted to demand temperature and starts, and the temperature that discharge outlet 6 is discharged is monitored that (it can be by draining
Thermometer is added at mouth 6 or at Sewage treatment container;Or discharge outlet 6 is connected directly in constant temperature water supply equipment, by right
The temperature of constant temperature water supply equipment reads the monitoring realized to 6 discharge temperature of discharge outlet), after up to the temperature, it meets experiment demand
Constant temperature water supply equipment can be closed, in this process, the warm water being filled in temperature adjustment pipeline 2 can be to depositing haemocoele 9 and insect takes chamber in
10 are heated and are kept the temperature, and will be simulated due to biological Membrane cover 4, positioned at the blood sample and suitable temperature deposited in haemocoele 9
Blood supply animal is formed, hematophagus voluntarily will draw the blood sample for being located at and depositing in haemocoele 9 by biological Membrane cover 4, to the process
After, insect outlet can be opened at this time by injecting ice water into temperature adjustment pipeline 2 to change environment locating for hematophagus
Afterwards, by the outlet of insect receiving bottle bottleneck alignment insect, hematophagus can be made to dissociate to get off and fully enter from biological Membrane cover 4
In insect receiving bottle;After hematophagus receives, the temperature adjustment pipeline 2 in blood shell 1, note blood channel 3 and biomembrane will be raised
Set 4 takes out together, is placed in the insect carefully pruned off and be hidden in biological Membrane cover 4 in ceramic whiteware basin, all collects;It has pruned off
The whole blood in haemocoele 9 is deposited in the extraction of Cheng Houyong syringe, is removed biological Membrane cover 4, is all placed in clinical waste collection vessel
In, then will raise blood shell 1, temperature adjustment pipeline 2 and note blood channel 3 and be placed in percent by volume solution is to impregnate in 65% to 75% alcohol
Disinfection is in case next use.When present invention is particularly suitable for needing to feed specific objective insect;In addition, working as this hair
The bright blood for feeding is the blood infected with virus, bacterium or other pathogenic microorganisms, or the purpose insect infection of feeding
There are virus, bacterium or other pathogenic microorganisms, it should (the Chinese people be total by " pathogenic microorganism Laboratory biosafety management rules "
With state State Council Decree the 424th) requirement carried out in corresponding laboratory, and disinfection treatment is carried out in accordance with regulations.Using this hair
It is bright to form blood supply animal by biological Membrane cover 4, positioned at the blood sample deposited in haemocoele 9 and suitable temperature simulation, make to inhale
Blood insect voluntarily can will draw the blood sample for being located at and depositing in haemocoele 9 by biological Membrane cover 4, can be by changing after the completion of it is sucked blood
Becoming its local environment can all exit into insect receiving bottle, easy to operate, easy to use, so that it is guaranteed that testing
Journey go on smoothly and the accuracy of experimental result.
Can blood device be raised to above-mentioned insect automatic fine and made further optimization and/or improvements according to actual needs:
Embodiment one: as shown in Fig. 1, raising blood shell 1 in a tubular form and there is the opening being mutually communicated in left and right ends, and is raising blood
Closure has the top plug 11 of energy plug in the left side opening of shell 1, and the bottom for having energy plug is blocked in the right openings for raising blood shell 1
Plug 12, top plug 11 and bottom plug 12 are elastic plug;Water filling port 5 and note blood mouth 7 are pierced by from top plug 11 and are located at top plug 11
Outside, discharge outlet 6 are pierced by from bottom plug 12 and are located at 12 outside of bottom plug;Temperature adjustment is sleeved on positioned at the biological Membrane cover 4 raised in blood shell 1
On the outside of the middle section of pipeline 2, and the left end sealing of biological Membrane cover 4 lock onto the right part outside and corresponding position for infusing blood channel 3
2 outside of temperature adjustment pipeline, the right end sealing of biological Membrane cover 4 lock onto 2 outside of temperature adjustment pipeline of corresponding position;Raising blood shell 1
Side wall is equipped with can take the side outlet that chamber 10 communicates in insect, and the corking 13 that can be removably mounted, corking 13 are equipped on side outlet
On be distributed with can make insect take in chamber 10 be connected to outside atmosphere and can prevent hematophagus escape ventilation sieve pore 14.At this
In embodiment, insect inlet is integrated with ventilation consent, and when needing to carry out the injection process of hematophagus, corking 13 is beaten
It opens, corking 13 is covered again to injection process completion, when needing to carry out the collection process of hematophagus, bottom plug 12 is beaten
It opens, process completion to be collected again covers bottom plug 12;Make top plug 11 and bottom plug 12 is that elastic plug can ensure that feeding blood shell
Body 1 is to be tightly connected with the intersection of temperature adjustment pipeline 2 and with the intersection for infusing blood channel 3;Using in a tubular form and left and right two
End has the feeding blood shell 1 for the opening being mutually communicated that can make the present embodiment easy to disassemble, disinfection and reuse.According to demand, it pushes up
Non-resilient corking 13 can also be used in plug 11, when it uses non-resilient corking 13, outside and note of the top plug 11 with temperature adjustment pipeline 2
The outside of blood channel 3 is both needed to injection sealed colloid 16 with plugging gap, prevents hematophagus from escaping, while it can be with feeding blood shell
What 1 left side opening was connected through a screw thread is installed together to enhance its switching performance.
Embodiment two: as shown in Fig. 2, raising blood shell 1 in a tubular form and there is the opening being mutually communicated in left and right ends, and is raising blood
The top plug 11 for having energy plug is blocked in the left side opening of shell 1, top plug 11 is elastic plug;In the right openings for raising blood shell 1
On the corking 13 that can be removably mounted is installed, being distributed in corking 13, which can make insect take chamber 10 in, is connected to outside atmosphere and can prevent
The ventilation sieve pore 14 of hematophagus escape;Water filling port 5, discharge outlet 6 and note blood mouth 7 are pierced by from top plug 11 and are located at top plug 11
Outside, U-tube shape of the temperature adjustment pipeline 2 in opening to the left, and the straight sections to link together with water filling port 5 are known as filling the water section;Position
It is sleeved on the outside of the middle part of water filling section in raising the biological Membrane cover 4 in blood shell 1, and the left end sealing of biological Membrane cover 4 lock onto note
On the outside of the right part of blood channel 3 and on the outside of the water filling section of corresponding position, the right end sealing of biological Membrane cover 4 lock onto corresponding position
It fills the water on the outside of section.In the present embodiment, insect inlet and insect outlet and ventilation sieve pore 14 are integrated, when being inhaled
When the injection and collection process of blood insect, corking 13 is opened, again covers corking 13 to process completion;Make top plug 11
It can ensure that the intersection for raising blood shell 1 and temperature adjustment pipeline 2 for elastic plug and be that sealing connects with the intersection of note blood channel 3
It connects;Using in a tubular form and the left and right ends feeding blood shell 1 that has the opening being mutually communicated can make the present embodiment easy to disassemble, disinfection and
It reuses.According to demand, 11 non-resilient corking 13 can also be used in top plug, when it uses non-resilient corking 13, top plug 11 with
The outside of temperature adjustment pipeline 2 and the outside for infusing blood channel 3 are both needed to injection sealed colloid 16 with plugging gap, prevent hematophagus from escaping
Ease, while it can be installed together with what the left side opening for raising blood shell 1 was connected through a screw thread to enhance its switching performance.
As shown in attached drawing 1,2, the temperature adjustment pipeline 2 and note 3 outer sheath of blood channel for corresponding to top 11 positions of plug are equipped with can be by the two
The casing 15 of limit is fettered, 15 sealing shroud of casing is filled in 11 loaded on top, and the both ends of casing 15 are piercing in a left side for top plug 11 respectively
Right two sides are marked with sealed colloid 16 in the gap of casing 15 and temperature adjustment pipeline 2 and note blood channel 3.By adding casing 15
The dismounting of temperature adjustment pipeline 2 and note blood channel 3 can be made more convenient, casing 15 can be made to form it by filling sealed colloid 16 good
Good constraint limit, at the same can plugging gap, effectively prevent hematophagus to escape.
As shown in attached drawing 1,2, corking 13 includes circle shape lid and gas-permeable nets, encloses in the middle part of shape lid and is equipped with gas-permeable nets,
Being distributed on gas-permeable nets can make insect take the ventilation sieve pore 14 that chamber 10 is connected to outside atmosphere and can prevent hematophagus from escaping in,
Gas-permeable nets are fine and closely woven thin,tough silk yarn or metallic sieve;It encloses shape lid and passes through end cap buckle 17 and corresponding side outlet or feeding blood shell 1
Right openings are installed together.The connection between corking 13 and side outlet can be made more stable by 17 connection of end cap buckle, no
Easily slip.According to demand, circle shape lid can also be installed together with side outlet by plug-in type structure, and gas-permeable nets can be with circle shape lid
Body is installed together or is integrally formed;Other well-known techniques existing made of metal or non-metallic material can be used in gas-permeable nets.
As shown in attached drawing 1,2, biological Membrane cover 4 is rat-tail biomembrane or artificial membrane;On the outside of the right part of note blood channel 3
And constraint is set with the rubber ring 18 in being closely enclosed in the two on the outside of the temperature adjustment pipeline 2 of corresponding position, biological Membrane cover 4
Left end, mounted in 18 outside of rubber ring, is set with anti-by circle 19 sealing shroud of shape clip between circle shape clip 19 and biological Membrane cover 4
Skidding circle 20;The right end of biological Membrane cover 4, mounted in 2 outside of temperature adjustment pipeline of corresponding position, is being enclosed by circle 19 sealing shroud of shape clip
Anti-skid washer 20 is set between shape clip 19 and biological Membrane cover 4.It can make biomembrane by circle shape clip 19 and anti-skid washer 20
The installation of set 4 is more firm, is not easy to slip;Add rubber ring 18 can make biological Membrane cover 4 left end and note blood channel 3 right part and
Good leakproofness is formed between the temperature adjustment pipeline 2 of corresponding position, prevents sample blood from oozing out from interface.According to demand, right
Being equipped on the outside of the anti-skid washer 20 for 19 installation site of circle shape clip that left side should be located at can make to enclose the ring that shape clip 19 is embedded within
Connected in star, corresponding be equipped on the outside of the anti-skid washer 20 of 19 installation site of circle shape clip on right side can make to enclose the insertion of shape clip 19
Annular groove in it;Biological Membrane cover 4 can be tubular Membrane cover or be formed by single film turnup.
Further include temperature automatically controlled water circle device 21 as shown in attached drawing 1,2, is equipped with temperature in temperature automatically controlled water circle device 21
Control component, temperature actuated panel and water can be pumped out to the circulating pump in water outlet 23, be set on temperature automatically controlled water circle device 21
There are water inlet 22 and water outlet 23, water outlet 23 is connected with water filling port 5 by connecting tube 24, and water inlet 22 and discharge outlet 6 are logical
Connecting tube 24 is crossed to be connected.According to demand, circulating pump can be peristaltic pump or other existing well-known techniques;Temperature automatically controlled water circulation
Thermostat water bath or other existing well-known techniques can be used in device 21.Temperature adjustment pipeline 2 and temperature automatically controlled water can in this way recycled to fill
21 formation water circulation is set, convenient for temperature adjusting and temperature monitoring;In addition, can be incited somebody to action when needing to inject ice water into temperature adjustment pipeline 2
The temperature of temperature automatically controlled water circle device 21 is adjusted to preference temperature, or connecting tube 24 is immersed in the container for filling ice water, from
And it makes it through rapid cooling and rapidly changes hematophagus local environment.
As shown in attached drawing 1,2, note blood mouth 7 is equipped with the note blood connector 25 to link together with note blood channel 3, infuses blood connector
The note blood glue plug 26 of energy plug is installed in 25.Note blood operation can be made more convenient laborsaving by adding note blood connector 25.According to
Demand, note blood connector 25 can be installed together or be integrally formed with note blood channel 3.
The above technical features constitute embodiments of the present invention, can basis with stronger adaptability and implementation result
Actual needs increases and decreases non-essential technical characteristic, to meet the needs of different situations.
The use process of the embodiment of the present invention one: first opening corking 13, and storage is had hematophagus to be tested
The opening of insect container is directed at side outlet, and fully entering all hematophaguses by slight jitter will plug after insect is taken in chamber 10
Lid 13 covers the injection process that hematophagus can be completed;Then note blood instrument connection note blood mouth 7 can be injected to depositing in haemocoele 9
Blood sample stops note blood operation after depositing the blood in haemocoele 9 and being full of, and is by the note closure of blood mouth 7 after note blood instrument is removed
Complete note blood process;The supply water temperature of temperature automatically controlled water circle device 21 is adjusted to demand temperature again and is started, and by certainly
The temperature of dynamic temperature control water circle device 21 reads the monitoring realized to 6 discharge temperature of discharge outlet, until its satisfaction experiment of the temperature
Can be turned off after demand, in this process, the warm water being filled in temperature adjustment pipeline 2 can to deposit haemocoele 9 and insect take in chamber 10 into
Row heating and heat preservation, due to biological Membrane cover 4, will simulate positioned at the blood sample and suitable temperature deposited in haemocoele 9 and to be formed
Blood supply animal, hematophagus voluntarily will draw the blood sample for being located at and depositing in haemocoele 9 by biological Membrane cover 4, to the end of the process
Afterwards, the container of ice water can be filled by lowering the supply water temperature of temperature automatically controlled water circle device 21 or by the immersion of connecting tube 24
In, so that making to enter in temperature adjustment pipeline 2 is cold water, passes through rapid cooling and rapidly changes hematophagus local environment,
After opening bottom plug 12 at this time, insect receiving bottle bottleneck is aligned to the right openings for raising blood shell 1, hematophagus can be made from biology
Dissociate to get off on Membrane cover 4 and fully enter in insect receiving bottle;After hematophagus receives, the tune in blood shell 1 will be raised
Warm pipeline 2, note blood channel 3 and biological Membrane cover 4 take out together, are placed in ceramic whiteware basin carefully to prune off and are hidden in biological Membrane cover 4
Insect, all collect;The whole blood in haemocoele 9 is deposited with syringe extraction after the completion of pruning off, removes biological Membrane cover 4,
It is all placed in clinical waste collection vessel, then will raise blood shell 1, temperature adjustment pipeline 2 and note blood channel 3 and be placed in volume basis
Than solution for soaking disinfection in 65% to 75% alcohol in case next use.Present invention is particularly suitable for need to specific objective
When insect is fed;In addition, when blood of the present invention for feeding is infected with virus, bacterium or other pathogenic microorganisms
Blood, or the purpose insect infection of feeding have virus, bacterium or other pathogenic microorganisms, should be by " pathogenic microorganism laboratory is raw
Object safety management regulations " requirement of (Decree of the State Council of the People's Republic of China the 424th) carries out in corresponding laboratory, and presses
Provide the processing that carries out disinfection.
Claims (16)
1. a kind of insect automatic fine raises blood device, it is characterised in that including raising blood shell, temperature adjustment pipeline, note blood channel and biomembrane
Set, raise blood shell in be equipped with temperature adjustment pipeline and note blood channel, the both ends of temperature adjustment pipeline be respectively water filling port and discharge outlet and
It stretches out in and raises outside blood shell, the partially sealed of temperature adjustment pipeline is enclosed in interior biological Membrane cover raising to have in blood shell;Infuse blood vessel
The one end in road, which stretches out in, raises that blood shell is outer and be known as infusing blood mouth, and the other end sealing for infusing blood channel penetrates in biological Membrane cover and is known as
Blood outlet;Feeding blood shell is to be tightly connected with the intersection of temperature adjustment pipeline and with the intersection for infusing blood channel, is raising blood shell
Body, which is equipped with, takes that chamber communicates and capable of opening and closing insect inlet and insect export in insect, and insect can be made by being equipped on raising blood shell
Take the ventilation sieve pore that chamber is connected to outside atmosphere and can prevent hematophagus from escaping in;On the outside of temperature adjustment pipeline and on the outside of note blood channel
And formation is deposited haemocoele, temperature adjustment pipeline outside and note blood channel outside and biology Membrane cover outside and is raised between biology Membrane cover inner cavity
Insect is formed between blood case inside takes chamber in.
2. insect automatic fine according to claim 1 raises blood device, it is characterised in that raise blood shell in a tubular form and left and right two
Holding has the opening being mutually communicated, and blocking in the left side opening for raising blood shell has the top of energy plug to fill in, on the right side for raising blood shell
The bottom plug for having energy plug is blocked in opening, top plug and bottom plug are elastic plug;From top, plug is pierced by simultaneously for water filling port and note blood mouth
Positioned at the outside of top plug, discharge outlet is pierced by from bottom plug and is located on the outside of bottom plug;It is sleeved on positioned at the intracorporal biological Membrane cover of blood shell is raised
On the outside of the middle section of temperature adjustment pipeline, and the left end sealing of biological Membrane cover lock onto the right part outside and corresponding position for infusing blood channel
On the outside of temperature adjustment pipeline, the right end sealing of biological Membrane cover is lock on the outside of the temperature adjustment pipeline of corresponding position;In the side wall for raising blood shell
The side outlet that chamber communicates can be taken in insect by being equipped with, and the corking that can be removably mounted is equipped on side outlet, is distributed in corking
Insect can be made to take the ventilation sieve pore that chamber is connected to outside atmosphere and can prevent hematophagus from escaping in.
3. insect automatic fine according to claim 1 raises blood device, it is characterised in that raise blood shell in a tubular form and left and right two
Holding has the opening being mutually communicated, and blocking in the left side opening for raising blood shell has the top of energy plug to fill in, and top plug is elastic plug;?
It raises and the corking that can be removably mounted is installed in the right openings of blood shell, being distributed in corking can make insect take chamber and outside atmosphere in
Connection and the ventilation sieve pore that can prevent hematophagus from escaping;From top, plug is pierced by and is located at top for water filling port, discharge outlet and note blood mouth
The outside of plug, U-tube shape of the temperature adjustment pipeline in opening to the left, and the straight sections to link together with water filling port are known as filling the water section;
It is sleeved on the outside of the middle part of water filling section positioned at the intracorporal biological Membrane cover of blood shell is raised, and the left end sealing of biological Membrane cover lock onto note blood
On the outside of the right part of pipeline and on the outside of the water filling section of corresponding position, the right end sealing of biological Membrane cover lock onto the water filling of corresponding position
Section outside.
4. insect automatic fine according to claim 2 or 3 raises blood device, it is characterised in that the temperature adjustment pipe of corresponding top plug position
Road and note blood channel outer sheath are equipped with the casing that the two can be fettered to limit, and bobbin seal is set in the plug of top, and the two of casing
End is piercing in the left and right sides of top plug respectively, is marked with sealed colloid in the gap of casing and temperature adjustment pipeline and note blood channel.
5. insect automatic fine according to claim 2 or 3 raises blood device, it is characterised in that corking is including circle shape lid and thoroughly
Gas net, enclose shape lid in the middle part of gas-permeable nets are installed, be distributed on gas-permeable nets can make insect take in chamber be connected to outside atmosphere and
The ventilation sieve pore that can prevent hematophagus from escaping, gas-permeable nets are fine and closely woven thin,tough silk yarn or metallic sieve;Shape lid is enclosed to buckle by end cap
It is installed together with corresponding side outlet or the right openings for raising blood shell.
6. insect automatic fine according to claim 4 raises blood device, it is characterised in that corking includes circle shape lid and breathes freely
Net encloses in the middle part of shape lid and is equipped with gas-permeable nets, and being distributed on gas-permeable nets can make insect take chamber in is connected to and energy with outside atmosphere
The ventilation sieve pore for preventing hematophagus from escaping, gas-permeable nets are fine and closely woven thin,tough silk yarn or metallic sieve;Enclose shape lid by end cap buckle and
Corresponding side outlet or the right openings for raising blood shell are installed together.
7. raising blood device according to insect automatic fine described in Claims 2 or 3 or 6, it is characterised in that biological Membrane cover is raw for rat-tail
Object film or artificial membrane;It fetters on the outside of the right part of note blood channel and on the outside of the temperature adjustment pipeline of corresponding position and is set with two
Person is closely enclosed in interior rubber ring, and the left end of biological Membrane cover is mounted on the outside of rubber ring by circle shape clip sealing shroud, in circle shape
Anti-skid washer is set between clip and biological Membrane cover;The right end of biological Membrane cover is mounted in corresponding position by circle shape clip sealing shroud
Temperature adjustment pipeline on the outside of, be set with anti-skid washer between circle shape clip and biological Membrane cover.
8. insect automatic fine according to claim 4 raises blood device, it is characterised in that biological Membrane cover be rat-tail biomembrane or
Artificial membrane;Constraint is set with the two is close on the outside of the right part of note blood channel and on the outside of the temperature adjustment pipeline of corresponding position
Be enclosed in interior rubber ring, the left end of biological Membrane cover by circle shape clip sealing shroud on the outside of rubber ring, circle shape clip with
Anti-skid washer is set between biological Membrane cover;The right end of biological Membrane cover is mounted in the temperature adjustment of corresponding position by circle shape clip sealing shroud
On the outside of pipeline, anti-skid washer is set between circle shape clip and biological Membrane cover.
9. insect automatic fine according to claim 5 raises blood device, it is characterised in that biological Membrane cover be rat-tail biomembrane or
Artificial membrane;Constraint is set with the two is close on the outside of the right part of note blood channel and on the outside of the temperature adjustment pipeline of corresponding position
Be enclosed in interior rubber ring, the left end of biological Membrane cover by circle shape clip sealing shroud on the outside of rubber ring, circle shape clip with
Anti-skid washer is set between biological Membrane cover;The right end of biological Membrane cover is mounted in the temperature adjustment of corresponding position by circle shape clip sealing shroud
On the outside of pipeline, anti-skid washer is set between circle shape clip and biological Membrane cover.
10. insect automatic fine according to claim 1 or 2 or 3 raises blood device, it is characterised in that further include temperature automatically controlled water
Circulator, temperature automatically controlled water circle device is interior to be equipped with temperature control component, temperature actuated panel and can pump out water to water outlet
Circulating pump, temperature automatically controlled water circle device is equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube
Logical, water inlet is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note to link together with note blood channel
Blood connector infuses the note blood glue plug that energy plug is equipped in blood connector.
11. insect automatic fine according to claim 4 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
12. insect automatic fine according to claim 5 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
13. insect automatic fine according to claim 6 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
14. insect automatic fine according to claim 7 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
15. insect automatic fine according to claim 8 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
16. insect automatic fine according to claim 9 raises blood device, it is characterised in that further include temperature automatically controlled water circulation dress
It sets, temperature control component, temperature actuated panel are equipped in temperature automatically controlled water circle device and water can be pumped out to the circulation to water outlet
Pump, temperature automatically controlled water circle device are equipped with inlet and outlet, and water outlet is connected with water filling port by connecting tube, intake
Mouth is connected with discharge outlet by connecting tube;Or/and note blood mouth is equipped with the note blood connector to link together with note blood channel,
The note blood glue plug of energy plug is installed in note blood connector.
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CN201610197846.8A CN105831018B (en) | 2016-03-31 | 2016-03-31 | Insect automatic fine raises blood device |
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CN201610197846.8A CN105831018B (en) | 2016-03-31 | 2016-03-31 | Insect automatic fine raises blood device |
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CN105831018A CN105831018A (en) | 2016-08-10 |
CN105831018B true CN105831018B (en) | 2019-02-26 |
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CN201610197846.8A Expired - Fee Related CN105831018B (en) | 2016-03-31 | 2016-03-31 | Insect automatic fine raises blood device |
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CN113409667B (en) * | 2021-06-21 | 2022-07-05 | 中国人民解放军陆军军医大学 | Device for simulating aortic blood spraying |
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US4850305A (en) * | 1987-12-02 | 1989-07-25 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
US5133289A (en) * | 1991-09-05 | 1992-07-28 | Cornell Research Foundation, Inc. | Artificial system and method for breeding fleas |
CN2393336Y (en) * | 1999-08-26 | 2000-08-30 | 中国人民解放军军事医学科学院微生物流行病研究所 | Blood supplier for bloodsuckers |
CN102812927B (en) * | 2012-06-21 | 2014-07-16 | 新疆维吾尔自治区疾病预防控制中心 | Automatic blood feeder for insect and application method thereof |
CN202773796U (en) * | 2012-09-19 | 2013-03-13 | 江苏省血吸虫病防治研究所 | Artificial blood feeding system for adult mosquitoes |
CN203646340U (en) * | 2013-12-13 | 2014-06-18 | 广州沃巴克生物科技有限公司 | Adult mosquito raising device |
CN205671280U (en) * | 2016-03-31 | 2016-11-09 | 新疆维吾尔自治区疾病预防控制中心 | Insect automatic fine raises blood device |
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