CN113519403A - Device for mouse sarcopenia modeling - Google Patents
Device for mouse sarcopenia modeling Download PDFInfo
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- CN113519403A CN113519403A CN202110766834.3A CN202110766834A CN113519403A CN 113519403 A CN113519403 A CN 113519403A CN 202110766834 A CN202110766834 A CN 202110766834A CN 113519403 A CN113519403 A CN 113519403A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
- A01K1/0356—Feeding or drinking devices associated with cages
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K5/00—Feeding devices for stock or game ; Feeding wagons; Feeding stacks
- A01K5/02—Automatic devices
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K7/00—Watering equipment for stock or game
- A01K7/02—Automatic devices ; Medication dispensers
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Abstract
The invention discloses a device for mouse sarcopenia modeling, which belongs to the field of mouse feeding and comprises a lower box body, an upper cover and a partition plate assembly; the partition plate assembly is arranged in the lower box body and is used for dividing the inner space of the lower box body into a plurality of mouse moving areas; the top of the mouse activity area is provided with a ventilation cover; the upper cover is covered on the top of the lower box body; the upper cover is provided with an air inlet and an air outlet. The device for the modeling of the sarcopenia of the mouse can limit the activity space of the mouse, is convenient to clean, automatically reads the water intake, the food intake and the activity of the mouse, eliminates the interference of other environmental conditions, and is convenient to model the sarcopenia of the mouse.
Description
Technical Field
The invention belongs to the field of mouse feeding, and particularly relates to a device for mouse sarcopenia modeling.
Background
Sarcopenia refers primarily to an age-related reduction in muscle mass, combined with a decrease in muscle strength and/or body function. With the progress of aging in society, sarcopenia has become a prominent problem affecting the health of the elderly, and research on the occurrence mechanism and prevention and treatment of sarcopenia is a research hotspot at present. The selection and establishment of the sarcopenia animal model are the basis for the development of related researches, and are particularly important in the research of sarcopenia of the elderly.
Due to the problems of complexity of aging, uncertainty of experimental intervention and the like, no animal modeling method similar to clinical sarcopenia pathology exists at present. At present, common mouse sarcopenia models are hind limb suspension method, joint position fixing method and the like, and the symptoms and pathology expressed by muscle attenuation are not consistent with those of sarcopenia of the old; the development of transgenic mice associated with sarcopenia and aging-accelerated model mice requires a longer period of time and the changes in the mouse muscle pathology can only partially mimic clinical sarcopenia.
The device aims to simulate an ideal model of skeletal muscle loss in human aging and simulate muscle mass reduction and muscle strength reduction caused by reduction of activity of old people. However, the conventional mouse breeding device cannot meet the requirement of experimental model building. A plurality of mice are usually bred in one breeding device, the internal space of the box body is relatively large, and the mice can easily train the muscles of the whole body by climbing the side wall of the breeding cage and the iron net for loading food materials and water bottles at the top, so that the requirement of muscle deficiency molding can not be met. Meanwhile, middle-aged and old mice bred by modeling are generally retired breeding mice, and male mice closed in the same cage position can have violent fighting behaviors to destroy the modeling environment.
Moreover, the existing rearing cages can not monitor the water intake, food intake and activity of each mouse, and are not beneficial to the collection and analysis of relevant experimental data. The excrement and urine of mouse and bedding material are mixed together, accumulate in the bottom of the box, all need take out the mouse when every clearance, and are very inconvenient.
Disclosure of Invention
The invention aims to provide a device for mouse sarcopenia modeling aiming at the defects, and solves the problems that no sarcopenia modeling device exists, the device is inconvenient to clean, no automatic monitoring system exists and the like. In order to achieve the purpose, the invention provides the following technical scheme:
the device for the hypomyofascitis modeling of the mice comprises a lower box body 1, an upper cover 2 and a clapboard component 3; the partition plate assembly 3 is arranged in the lower box body 1 and is used for dividing the inner space of the lower box body 1 into a plurality of mouse moving areas 4; the top of the mouse moving area 4 is provided with a ventilation cover 5; the upper cover 2 covers the top of the lower box body 1; the upper cover 2 is provided with an air inlet 6 and an air outlet 7. According to the structure, the partition plate component 3 divides the inner space of the lower box body 1 into the plurality of mouse moving areas 4, and one mouse is placed in each mouse moving area 4, so that the mice cannot be mixed together, the molding mice are prevented from fighting, and the molding environment is prevented from being damaged. 4 tops in mouse active area are equipped with ventilative lid 5, and ventilative lid 5 makes mouse active area 4 ventilate, and ventilative lid 5 restriction mouse up climbs in addition, ensures that the mouse just is fit for the mouse and turns round in a limited little space, prevents that the mouse from lifting the head and climbing, is different from a crowd of mice in a big space, and little space is convenient for little disease of mouse muscle and is made the mould. This device is placed in central animal room SPF level barrier system, and malleation air supply system is connected to air inlet 6 on the upper cover 2, and clean air gets into in the upper cover 2 from air inlet 6 to permeate through ventilative lid 5 and get into mouse active area 4, provide clean air for mouse active area 4, then 7 exhaust air in the gas outlet, ensure that the mouse is in a good living environment, avoid other germ to disturb little disease modeling of mouse flesh. The upper cover 2 is detachably connected with the lower box body 1 through a buckle.
Further, the lower case 1 includes a side case 8 and a lower cover 9; the lower cover 9 is arranged below the side shell 8 in an openable way; an isolation net 10 is arranged below the mouse active region 4; the separation net 10 and the side casing 8 are fixed. According to the structure, the side shell 8 is formed by connecting four side walls end to end, the upper part is connected with the upper cover 2, and the lower part is connected with the lower cover 9; the lower cover 9 and one side wall of the side shell 8 are hinged and buckled on the opposite side wall through a buckle; the mouse moves on the isolation net 10, the lower cover 9 is supported below the isolation net 10, padding is paved on the isolation net 10, and the padding is fresh wood shavings or corncobs; when the excrement of the mouse falls on the padding and needs to be cleaned, the padding and the excrement leak down from the isolation net 10 only by opening the lower cover 9, so that the padding can be quickly replaced and the excrement can be quickly treated without grabbing the mouse out.
Further, the diaphragm assembly 3 includes one longitudinal plate 11 and five transverse plates 12; the five transverse plates 12 are parallel to each other and are vertical to the longitudinal plate 11, so that the inner space of the lower box body 1 is divided into twelve mouse moving areas 4; each mouse active region 4 contacts the side walls of the side casing 8; the longitudinal plate 11 and the transverse plate 12 are both provided with communicating holes 13, so that the adjacent mouse moving areas 4 are communicated. According to the structure, the internal space of the lower box body 1 is divided into twelve mouse moving areas 4, and the modeling of twelve mice is carried out simultaneously, so that the requirements of most mouse experiments on the number of cases of an experimental group and a control group are met. Every mouse active region 4 all contacts the lateral wall of lateral shell 8, is convenient for drink water through the lateral wall and feeds and eat, avoids drinking water device, feeds and eats the device setting in mouse active region 4 to guarantee that mouse active region 4 is a less space, do benefit to the little disease of mouse and make the mould. The communicating holes 13 are formed in the longitudinal plate 11 and the transverse plate 12, so that the adjacent mouse moving areas 4 are communicated, communication between the adjacent mice is facilitated, and psychological obstacle of the model to the mice is avoided.
Furthermore, the top of each mouse active area 4 is independently provided with a ventilation cover 5; the air-permeable cover 5 is hinged with the top of the clapboard component 3; a barrier strip 14 is arranged on the inner wall of the side shell 8; the ventilation cover 5 is detachably connected with the barrier strip 14 through a buckle. According to the structure, the top of each mouse activity area 4 is independently provided with the air permeable cover 5, the air permeable cover 5 can be opened from the upper side and cannot be opened by a mouse below, and the corresponding mouse can be put in or taken out by opening the air permeable cover 5 corresponding to the mouse activity area 4, so that the mouse in other mouse activity areas 4 cannot run out.
Further, the device comprises a controller, a plurality of drinking devices 15, feeding devices 16 and behavior monitoring devices 17 which are in one-to-one correspondence with the mouse activity area 4; the drinking water device 15 is used for providing drinking water for the mice corresponding to the mouse active area 4; the feeding device 16 is used for providing food of the mouse corresponding to the mouse active area 4; the behavior monitoring device 17 is used for monitoring the activity of the mouse corresponding to the mouse activity zone 4.
Further, the drinking device 15 comprises a bottle body 18 and a tube body 19; a plug 20 is fixed on the bottle body 18; the outer side wall of the side shell 8 is provided with a slot 21 corresponding to the plug 20; the plug 20 is inserted into the slot 21 to detachably fix the bottle body 18 on the outer side wall of the side shell 8; the tube 19 is located at the bottom of the bottle 18 and extends into the corresponding mouse active region 4. According to the structure, the water is filled in the bottle body 18, and the water can flow to the mouse moving area 4 through the pipe body 19, so that the mouse in the mouse moving area 4 can drink the water conveniently. The plug 20 is inserted into the slot 21 to detachably fix the bottle body 18 on the outer side wall of the side shell 8, so as to prevent the bottle body 18 from shaking randomly.
Further, the tube 19 comprises a bellows 22, a cannula 23, a first spring 24, a water-absorbing bead 25, a limiting ring 26, an outer edge ring 27 and an inner edge ring 28; the insertion pipe 23 penetrates through a first through hole 29 formed in the side wall of the side shell 8; a sealing sleeve 30 is arranged in the first through hole 29; the insertion pipe 23 is hermetically connected with the side wall of the side shell 8 through a sealing sleeve 30; an outer edge ring 27 and an inner edge ring 28 are arranged on the outer wall of the insertion pipe 23; the outer edge ring 27 blocks the outer side of the first through hole 29, and the inner edge ring 28 blocks the inner side of the first through hole 29; the outer end of the cannula 23 is connected with the bottom of the bottle body 18 through a corrugated pipe 22, and the inner end is provided with a conical port 31; the conical surface opening 31 gradually shrinks inwards; a limiting ring 26 is arranged on the inner wall of the insertion pipe 23; a first spring 24 is arranged between the limiting ring 26 and the water absorption bead 25; the first spring 24 is used for pushing the water absorption ball 25 on the conical surface opening 31; the water absorption beads 25 comprise washing cotton at the inner layer and a metal spherical shell wrapping the washing cotton; and a plurality of water holes are formed in the metal spherical shell. As can be seen from the above structure, the bottle body 18 is fixed on the outer side wall of the side casing 8, and the cannula 23 can be easily inserted into the sealing sleeve 30 of the first through hole 29 because the bellows 22 is flexible; because the sealing sleeve 30 is soft, the inner edge ring 28 on the cannula 23 can be plugged into the first through hole 29 under the action of applied force, while the outer edge ring 27 stops outside the first through hole 29, so that the cannula 23 cannot be plugged and pulled without applying a large force; the first spring 24 is used for pushing the water absorption bead 25 against the conical surface opening 31, the water absorption bead 25 cannot fall out because the conical surface opening 31 is gradually reduced inwards, when a mouse licks the water absorption bead 25, the water absorption bead 25 can be compressed inwards, so that water can be reserved in the mouth of the mouse, and when the mouse does not drink water, the water absorption bead blocks the conical surface opening 31, so that water loss is avoided, and the water drinking amount of the mouse can be accurately measured; the water in the cannula 23 can infiltrate the washing cotton through the water holes, so that the water on the surface of the water absorption bead 25 is kept, but the water cannot drip randomly, and the mouse can be guided to drink the water.
Further, a liquid level sensor 32 is arranged on the bottle body 18; the liquid level sensor 32 is used for monitoring the liquid level of the water in the bottle body 18; the level sensor 32 is electrically connected to the controller. According to the structure, the liquid level sensor 32 transmits the liquid level information before and after the monitoring time period to the controller, and the controller can accurately calculate the water intake of the mouse in the monitoring time period.
Further, the feeding device 16 comprises a displacement sensor 33, a food bar 34, a sleeve 35, a top cover and a second spring 37; the sleeve 35 penetrates through a second through hole 38 formed in the side wall of the side shell 8 and extends into the corresponding mouse moving area 4; a food bar 34 is arranged in the sleeve 35, and the food bar 34 extends out of the inner end of the sleeve 35; an openable top cover is arranged at the outer end of the sleeve 35, and an L-shaped baffle 39 is arranged at the inner end; a displacement sensor 33 is arranged on the top cover; a second spring 37 is arranged between the food bar 34 and the top cover; the second spring 37 is used for pushing the food bar 34 against the L-shaped stop 39; the L-shaped baffle 39 and the inner end of the sleeve 35 form a notch; the displacement sensor 33 is electrically connected to the controller. According to the structure, the inner wall of the sleeve 35 is cylindrical, the food bar 34 is also cylindrical, the food bar 34 extends out of the inner end of the sleeve 35, and the food bar 34 cannot fall off due to the action of the L-shaped baffle 39; because the L-shaped baffle 39 and the inner end of the sleeve 35 form a gap, a mouse can bite the food stick at the gap, when the food stick is completely bitten by the leakage part, the food stick 34 is continuously pushed out under the action of the second spring 37, so that the mouse can be continuously fed; the L-shaped stop 39 is an L-shaped plate; the displacement sensor 33 reads the displacement information of the food bar 34 in the monitoring time period and transmits the information to the controller, and the controller can accurately calculate the feeding amount of the mouse in the monitoring time period. The cover can be opened to facilitate insertion of the food bar 34 into the sleeve 35.
Further, the behavior monitoring device 17 includes a camera; the camera is positioned right above the corresponding mouse moving area 4 and is fixed on the upper cover 2; the camera is electrically connected with the controller; the ventilating cover 5 and the clapboard component 3 are made of transparent materials. According to the structure, the monitoring device 17 adopts a Smart series small animal behavior recording and analyzing system developed by Panlab corporation, and the system consists of a camera, a computer, data acquisition and analysis software and a data acquisition card. The computer, the data acquisition analysis software and the data acquisition card are used as a part of the controller of the device, the camera shoots the activity of the mouse, the data is transmitted to the controller, and the controller can obtain the activity of the mouse through calculation. The air permeable cover 5, the clapboard component 3, the lower box body 1 and the upper cover 2 are made of acrylic glass material or PVC plastic material.
The invention has the beneficial effects that:
the invention discloses a device for mouse sarcopenia modeling, which belongs to the field of mouse feeding and comprises a lower box body, an upper cover and a partition plate assembly; the partition plate assembly is arranged in the lower box body and is used for dividing the inner space of the lower box body into a plurality of mouse moving areas; the top of the mouse activity area is provided with a ventilation cover; the upper cover is covered on the top of the lower box body; the upper cover is provided with an air inlet and an air outlet. The device for the modeling of the sarcopenia of the mouse can limit the activity space of the mouse, is convenient to clean, automatically reads the water intake, the food intake and the activity of the mouse, eliminates the interference of other environmental conditions, and is convenient to model the sarcopenia of the mouse.
Drawings
FIG. 1 is a schematic top view of the present invention with the partition assembly broken away and the upper and venting covers removed;
FIG. 2 is a front view of the present invention in cross-section with the feeding set removed;
FIG. 3 is a schematic view of the front view of the present invention with the drinking device removed;
FIG. 4 is a schematic cross-sectional view of the tube of the present invention;
fig. 5 is a schematic view of a feeding device of the present invention;
in the drawings: 1-lower box body, 2-upper cover, 3-clapboard component, 4-mouse active area, 5-ventilating cover, 6-air inlet, 7-air outlet, 8-side shell, 9-lower cover, 10-isolation net, 11-longitudinal plate, 12-transverse plate, 13-communicating hole, 14-barrier strip, 15-drinking device, 16-feeding device, 17-behavior monitoring device, 18-bottle body, 19-tube body, 20-plug, 21-slot, 22-corrugated tube, 23-cannula, 24-first spring, 25-water-absorbing bead, 26-spacing ring, 27-outer edge ring, 28-inner edge ring, 29-first through hole, 30-sealing sleeve, 31-conical port, 32-liquid level sensor, 33-displacement sensor, 34-food bar, 35-sleeve, 37-second spring, 38-second through hole, 39-L-shaped stop.
Detailed Description
The present invention will be described in further detail below with reference to the drawings and the embodiments, but the present invention is not limited to the following examples.
The first embodiment is as follows:
see figures 1-5. The device for the hypomyofascitis modeling of the mice comprises a lower box body 1, an upper cover 2 and a clapboard component 3; the partition plate assembly 3 is arranged in the lower box body 1 and is used for dividing the inner space of the lower box body 1 into a plurality of mouse moving areas 4; the top of the mouse moving area 4 is provided with a ventilation cover 5; the upper cover 2 covers the top of the lower box body 1; the upper cover 2 is provided with an air inlet 6 and an air outlet 7. According to the structure, the partition plate component 3 divides the inner space of the lower box body 1 into the plurality of mouse moving areas 4, and one mouse is placed in each mouse moving area 4, so that the mice cannot be mixed together, the molding mice are prevented from fighting, and the molding environment is prevented from being damaged. 4 tops in mouse active area are equipped with ventilative lid 5, and ventilative lid 5 makes mouse active area 4 ventilate, and ventilative lid 5 restriction mouse up climbs in addition, ensures that the mouse just is fit for the mouse and turns round in a limited little space, prevents that the mouse from lifting the head and climbing, is different from a crowd of mice in a big space, and little space is convenient for little disease of mouse muscle and is made the mould. This device is placed in central animal room SPF level barrier system, and malleation air supply system is connected to air inlet 6 on the upper cover 2, and clean air gets into in the upper cover 2 from air inlet 6 to permeate through ventilative lid 5 and get into mouse active area 4, provide clean air for mouse active area 4, then 7 exhaust air in the gas outlet, ensure that the mouse is in a good living environment, avoid other germ to disturb little disease modeling of mouse flesh. The upper cover 2 is detachably connected with the lower box body 1 through a buckle.
Example two:
see figures 1-5. On the basis of the first embodiment, the lower case 1 comprises a side shell 8 and a lower cover 9; the lower cover 9 is arranged below the side shell 8 in an openable way; an isolation net 10 is arranged below the mouse active region 4; the separation net 10 and the side casing 8 are fixed. According to the structure, the side shell 8 is formed by connecting four side walls end to end, the upper part is connected with the upper cover 2, and the lower part is connected with the lower cover 9; the lower cover 9 and one side wall of the side shell 8 are hinged and buckled on the opposite side wall through a buckle; the mouse moves on the isolation net 10, the lower cover 9 is supported below the isolation net 10, padding is paved on the isolation net 10, and the padding is fresh wood shavings or corncobs; when the excrement of the mouse falls on the padding and needs to be cleaned, the padding and the excrement leak down from the isolation net 10 only by opening the lower cover 9, so that the padding can be quickly replaced and the excrement can be quickly treated without grabbing the mouse out.
The diaphragm assembly 3 comprises a longitudinal plate 11 and five transverse plates 12; the five transverse plates 12 are parallel to each other and are vertical to the longitudinal plate 11, so that the inner space of the lower box body 1 is divided into twelve mouse moving areas 4; each mouse active region 4 contacts the side walls of the side casing 8; the longitudinal plate 11 and the transverse plate 12 are both provided with communicating holes 13, so that the adjacent mouse moving areas 4 are communicated. According to the structure, the internal space of the lower box body 1 is divided into twelve mouse moving areas 4, and the modeling of twelve mice is carried out simultaneously, so that the requirements of most mouse experiments on the number of cases of an experimental group and a control group are met. Every mouse active region 4 all contacts the lateral wall of lateral shell 8, is convenient for drink water through the lateral wall and feeds and eat, avoids drinking water device, feeds and eats the device setting in mouse active region 4 to guarantee that mouse active region 4 is a less space, do benefit to the little disease of mouse and make the mould. The communicating holes 13 are formed in the longitudinal plate 11 and the transverse plate 12, so that the adjacent mouse moving areas 4 are communicated, communication between the adjacent mice is facilitated, and psychological obstacle of the model to the mice is avoided.
The top of each mouse active area 4 is independently provided with a ventilation cover 5; the air-permeable cover 5 is hinged with the top of the clapboard component 3; a barrier strip 14 is arranged on the inner wall of the side shell 8; the ventilation cover 5 is detachably connected with the barrier strip 14 through a buckle. According to the structure, the top of each mouse activity area 4 is independently provided with the air permeable cover 5, the air permeable cover 5 can be opened from the upper side and cannot be opened by a mouse below, and the corresponding mouse can be put in or taken out by opening the air permeable cover 5 corresponding to the mouse activity area 4, so that the mouse in other mouse activity areas 4 cannot run out.
Example three:
see figures 1-5. On the basis of the second embodiment, the device further comprises a controller, a plurality of drinking devices 15, feeding devices 16 and behavior monitoring devices 17 which are in one-to-one correspondence with the mouse activity areas 4; the drinking water device 15 is used for providing drinking water for the mice corresponding to the mouse active area 4; the feeding device 16 is used for providing food of the mouse corresponding to the mouse active area 4; the behavior monitoring device 17 is used for monitoring the activity of the mouse corresponding to the mouse activity zone 4.
The drinking device 15 comprises a bottle body 18 and a pipe body 19; a plug 20 is fixed on the bottle body 18; the outer side wall of the side shell 8 is provided with a slot 21 corresponding to the plug 20; the plug 20 is inserted into the slot 21 to detachably fix the bottle body 18 on the outer side wall of the side shell 8; the tube 19 is located at the bottom of the bottle 18 and extends into the corresponding mouse active region 4. According to the structure, the water is filled in the bottle body 18, and the water can flow to the mouse moving area 4 through the pipe body 19, so that the mouse in the mouse moving area 4 can drink the water conveniently. The plug 20 is inserted into the slot 21 to detachably fix the bottle body 18 on the outer side wall of the side shell 8, so as to prevent the bottle body 18 from shaking randomly.
The pipe body 19 comprises a corrugated pipe 22, a cannula 23, a first spring 24, a water absorption bead 25, a limiting ring 26, an outer edge ring 27 and an inner edge ring 28; the insertion pipe 23 penetrates through a first through hole 29 formed in the side wall of the side shell 8; a sealing sleeve 30 is arranged in the first through hole 29; the insertion pipe 23 is hermetically connected with the side wall of the side shell 8 through a sealing sleeve 30; an outer edge ring 27 and an inner edge ring 28 are arranged on the outer wall of the insertion pipe 23; the outer edge ring 27 blocks the outer side of the first through hole 29, and the inner edge ring 28 blocks the inner side of the first through hole 29; the outer end of the cannula 23 is connected with the bottom of the bottle body 18 through a corrugated pipe 22, and the inner end is provided with a conical port 31; the conical surface opening 31 gradually shrinks inwards; a limiting ring 26 is arranged on the inner wall of the insertion pipe 23; a first spring 24 is arranged between the limiting ring 26 and the water absorption bead 25; the first spring 24 is used for pushing the water absorption ball 25 on the conical surface opening 31; the water absorption beads 25 comprise washing cotton at the inner layer and a metal spherical shell wrapping the washing cotton; and a plurality of water holes are formed in the metal spherical shell. As can be seen from the above structure, the bottle body 18 is fixed on the outer side wall of the side casing 8, and the cannula 23 can be easily inserted into the sealing sleeve 30 of the first through hole 29 because the bellows 22 is flexible; because the sealing sleeve 30 is soft, the inner edge ring 28 on the cannula 23 can be plugged into the first through hole 29 under the action of applied force, while the outer edge ring 27 stops outside the first through hole 29, so that the cannula 23 cannot be plugged and pulled without applying a large force; the first spring 24 is used for pushing the water absorption bead 25 against the conical surface opening 31, the water absorption bead 25 cannot fall out because the conical surface opening 31 is gradually reduced inwards, when a mouse licks the water absorption bead 25, the water absorption bead 25 can be compressed inwards, so that water can be reserved in the mouth of the mouse, and when the mouse does not drink water, the water absorption bead blocks the conical surface opening 31, so that water loss is avoided, and the water drinking amount of the mouse can be accurately measured; the water in the cannula 23 can infiltrate the washing cotton through the water holes, so that the water on the surface of the water absorption bead 25 is kept, but the water cannot drip randomly, and the mouse can be guided to drink the water.
A liquid level sensor 32 is arranged on the bottle body 18; the liquid level sensor 32 is used for monitoring the liquid level of the water in the bottle body 18; the level sensor 32 is electrically connected to the controller. According to the structure, the liquid level sensor 32 transmits the liquid level information before and after the monitoring time period to the controller, and the controller can accurately calculate the water intake of the mouse in the monitoring time period.
The feeding device 16 comprises a displacement sensor 33, a food bar 34, a sleeve 35, a top cover and a second spring 37; the sleeve 35 penetrates through a second through hole 38 formed in the side wall of the side shell 8 and extends into the corresponding mouse moving area 4; a food bar 34 is arranged in the sleeve 35, and the food bar 34 extends out of the inner end of the sleeve 35; an openable top cover is arranged at the outer end of the sleeve 35, and an L-shaped baffle 39 is arranged at the inner end; a displacement sensor 33 is arranged on the top cover; a second spring 37 is arranged between the food bar 34 and the top cover; the second spring 37 is used for pushing the food bar 34 against the L-shaped stop 39; the L-shaped baffle 39 and the inner end of the sleeve 35 form a notch; the displacement sensor 33 is electrically connected to the controller. According to the structure, the inner wall of the sleeve 35 is cylindrical, the food bar 34 is also cylindrical, the food bar 34 extends out of the inner end of the sleeve 35, and the food bar 34 cannot fall off due to the action of the L-shaped baffle 39; because the L-shaped baffle 39 and the inner end of the sleeve 35 form a gap, a mouse can bite the food stick at the gap, when the food stick is completely bitten by the leakage part, the food stick 34 is continuously pushed out under the action of the second spring 37, so that the mouse can be continuously fed; the L-shaped stop 39 is an L-shaped plate; the displacement sensor 33 reads the displacement information of the food bar 34 in the monitoring time period and transmits the information to the controller, and the controller can accurately calculate the feeding amount of the mouse in the monitoring time period. The cover can be opened to facilitate insertion of the food bar 34 into the sleeve 35.
The behavior monitoring device 17 comprises a camera; the camera is positioned right above the corresponding mouse moving area 4 and is fixed on the upper cover 2; the camera is electrically connected with the controller; the ventilating cover 5 and the clapboard component 3 are made of transparent materials. According to the structure, the monitoring device 17 adopts a Smart series small animal behavior recording and analyzing system developed by Panlab corporation, and the system consists of a camera, a computer, data acquisition and analysis software and a data acquisition card. The computer, the data acquisition analysis software and the data acquisition card are used as a part of the controller of the device, the camera shoots the activity of the mouse, the data is transmitted to the controller, and the controller can obtain the activity of the mouse through calculation. The air permeable cover 5, the clapboard component 3, the lower box body 1 and the upper cover 2 are made of acrylic glass material or PVC plastic material.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A device that is used for little disease of mouse muscle to make mould which characterized in that: comprises a lower box body (1), an upper cover (2) and a clapboard component (3); the partition plate assembly (3) is arranged in the lower box body (1) and is used for dividing the inner space of the lower box body (1) into a plurality of mouse moving areas (4); the top of the mouse moving area (4) is provided with a ventilation cover (5); the upper cover (2) covers the top of the lower box body (1); an air inlet (6) and an air outlet (7) are arranged on the upper cover (2).
2. The device for sarcopenia modelling according to claim 1 wherein: the lower box body (1) comprises a side shell (8) and a lower cover (9); the lower cover (9) is arranged below the side shell (8) in an openable way; an isolation net (10) is arranged below the mouse active region (4); the isolation net (10) is fixed with the side shell (8).
3. The device for mouse sarcopenia modelling according to claim 2 wherein: the diaphragm assembly (3) comprises a longitudinal plate (11) and five transverse plates (12); the five transverse plates (12) are parallel to each other and are vertical to the longitudinal plate (11), so that the inner space of the lower box body (1) is divided into twelve mouse moving areas (4); each mouse active zone (4) contacts the side walls of the side casing (8); the longitudinal plate (11) and the transverse plate (12) are both provided with communicating holes (13) so that the adjacent mouse moving areas (4) are communicated.
4. The device for sarcopenia modelling according to claim 3 wherein: the top of each mouse moving area (4) is independently provided with a ventilation cover (5); the top of the ventilation cover (5) is hinged with the top of the clapboard component (3); a baffle strip (14) is arranged on the inner wall of the side shell (8); the ventilation cover (5) is detachably connected with the barrier strip (14) through a buckle.
5. The device for sarcopenia modelling according to claim 3 wherein: the mouse feeding device also comprises a controller, a plurality of drinking devices (15), feeding devices (16) and behavior monitoring devices (17), wherein the drinking devices (15), the feeding devices and the behavior monitoring devices are in one-to-one correspondence with the mouse moving areas (4); the drinking device (15) is used for providing drinking water for the mice corresponding to the mouse active area (4); the feeding device (16) is used for providing food of the mice corresponding to the mouse active area (4); the behavior monitoring device (17) is used for monitoring the activity degree of the mouse corresponding to the mouse activity area (4).
6. The device for hypomyogenesis in mice according to claim 5, wherein: the drinking device (15) comprises a bottle body (18) and a pipe body (19); a plug (20) is fixed on the bottle body (18); the outer side wall of the side shell (8) is provided with a slot (21) corresponding to the plug (20); the plug (20) is inserted into the slot (21) to detachably fix the bottle body (18) on the outer side wall of the side shell (8); the tube body (19) is positioned at the bottom of the bottle body (18) and extends into the corresponding mouse moving area (4).
7. The device for hypomyogenesis in mice according to claim 6, wherein: the pipe body (19) comprises a corrugated pipe (22), an insertion pipe (23), a first spring (24), water absorption beads (25), a limiting ring (26), an outer edge ring (27) and an inner edge ring (28); the insertion pipe (23) penetrates through a first through hole (29) formed in the side wall of the side shell (8); a sealing sleeve (30) is arranged in the first through hole (29); the insertion pipe (23) is hermetically connected with the side wall of the side shell (8) through a sealing sleeve (30); an outer edge ring (27) and an inner edge ring (28) are arranged on the outer wall of the insertion pipe (23); the outer edge ring (27) is blocked outside the first through hole (29), and the inner edge ring (28) is blocked inside the first through hole (29); the outer end of the cannula (23) is connected with the bottom of the bottle body (18) through a corrugated pipe (22), and the inner end of the cannula is provided with a conical surface opening (31); the conical surface opening (31) gradually shrinks inwards; a limiting ring (26) is arranged on the inner wall of the insertion pipe (23); a first spring (24) is arranged between the limiting ring (26) and the water absorption bead (25); the first spring (24) is used for propping the water absorption bead (25) on the conical surface opening (31); the water absorption beads (25) comprise washing cotton at the inner layer and a metal spherical shell wrapping the washing cotton; and a plurality of water holes are formed in the metal spherical shell.
8. The device for sarcopenia modelling according to claim 7 wherein: a liquid level sensor (32) is arranged on the bottle body (18); the liquid level sensor (32) is used for monitoring the liquid level of water in the bottle body (18); the liquid level sensor (32) is electrically connected with the controller.
9. The device for hypomyogenesis in mice according to claim 5, wherein: the feeding device (16) comprises a displacement sensor (33), a food bar (34), a sleeve (35), a top cover and a second spring (37); the sleeve (35) penetrates through a second through hole (38) formed in the side wall of the side shell (8) and extends into the corresponding mouse moving area (4); a food bar (34) is arranged in the sleeve (35), and the food bar (34) extends out of the inner end of the sleeve (35); an openable top cover is arranged at the outer end of the sleeve (35), and an L-shaped stop (39) is arranged at the inner end of the sleeve; a displacement sensor (33) is arranged on the top cover; a second spring (37) is arranged between the food bar (34) and the top cover; the second spring (37) is used for pushing the food bar (34) against the L-shaped stop (39); the L-shaped baffle (39) and the inner end of the sleeve (35) form a notch; the displacement sensor (33) is electrically connected with the controller.
10. The device for hypomyogenesis in mice according to claim 5, wherein: the behavior monitoring device (17) comprises a camera; the camera is positioned right above the corresponding mouse moving area (4) and is fixed on the upper cover (2); the camera is electrically connected with the controller; the ventilating cover (5) and the clapboard component (3) are made of transparent materials.
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| CN113519403B (en) | 2022-09-27 |
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