CN215799612U

CN215799612U - Embryo culture dish

Info

Publication number: CN215799612U
Application number: CN202120580631.0U
Authority: CN
Inventors: 董玲凤
Original assignee: Foshan Maternal and Child Health Care Hospital
Current assignee: Foshan Maternal and Child Health Care Hospital
Priority date: 2021-03-22
Filing date: 2021-03-22
Publication date: 2022-02-11
Anticipated expiration: 2031-03-22

Abstract

The utility model discloses an embryo culture dish, which comprises a culture dish body and a culture dish cover; the culture dish cover is connected with the upper part of the culture dish body; the culture dish body is provided with a liquid drop accommodating area; the liquid drop accommodating area comprises one or more culture modules, and the culture modules are provided with a plurality of culture holes; the side walls adjacent to the culture holes are provided with through holes, the through holes are used for communicating the culture holes, and the specification of the through holes is smaller than the size of embryos in the culture holes. The culture module can trace and score a single embryo during embryo collection culture, and is beneficial to selecting out embryos with more potential; besides, the efficiency of liquid drop preparation can be improved, and liquid drop identification marks can be conveniently identified; the embryo culture dish disclosed by the utility model is also suitable for other cells needing co-culture.

Description

Embryo culture dish

Technical Field

The utility model relates to the field of cell culture dishes, in particular to an embryo culture dish.

Background

In the assisted pregnancy cycle of the assisted tube infant, the early embryo formed after the combination of sperm and ovum needs to be cultured in vitro for 3-6 days (Day of aspiration is recorded as Day 0). The embryo in vitro culture method adopted by most of the current assisted reproductive centers comprises two stages, wherein the first stage is that each embryo is independently cultured and scored (Day1-Day3), and the first stage needs to make a culture drop of 20-50 mu L in a culture dish and put one embryo for culture; the second stage is a collective culture (Day3-Day6), which is a blastocyst culture stage, and is to select 2-6 individually cultured available Day3 embryos for collective culture, and a plurality of Day3 embryos are placed in a drop of 50-100 μ L for culture; a large number of documents show that embryo collective culture is beneficial to the formation of blastocysts, and probably the embryos secrete substances such as growth factors, cytokines, hormone molecules and the like after starting the expression of self genomes, so that the growth and development of adjacent embryos can be promoted, and therefore, the embryo collective culture mode can improve the blastocyst formation rate and the available blastocyst rate.

The culture dish mainly used by most of the assisted reproductive centers at present is a cylindrical culture dish with the diameter of 35mm and the height of 10mm, and the method comprises the following steps when liquid drops are prepared: firstly, marking the bottom of a culture dish for distinguishing different embryos; then sucking a proper amount of culture solution by using a Pasteur pipette, and dripping culture solution drops at each mark position; finally, 2.5mL of mineral oil was added, the dish lid was closed, carefully placed in a carbon dioxide incubator, and equilibrated overnight for use. The current culture dish can not trace back and individually score a single embryo in a blastocyst stage when carrying out embryo collective culture, thereby preventing an embryologist from collecting development information of the embryo in a blastocyst formation stage and being not beneficial to selecting the embryo with more potential; 2. during preparation, because of errors in manual operation, the sizes of all culture liquid drops are difficult to ensure to be consistent, and quality control in clinical work is not facilitated; 3. when the culture dish is moved, the liquid drops are easy to adhere to each other, so that the observation and the recording of the follow-up embryo are not facilitated; 4. the culture liquid drop is manufactured by using the conventional culture dish, the efficiency is low, the labor is consumed, the time is long, and the risk of the culture liquid being polluted is increased due to the long liquid preparation time.

It is seen that improvements and enhancements to the prior art are needed.

SUMMERY OF THE UTILITY MODEL

In view of the defects of the prior art, the utility model aims to provide an embryo culture dish, and aims to solve the problem that a single embryo cannot be traced in the process of embryo collection culture in the prior art.

In order to achieve the purpose, the utility model adopts the following technical scheme:

an embryo culture dish comprises a culture dish body and a culture dish cover; the culture dish cover is connected to the culture dish body in a covering mode; the culture dish body is provided with a liquid drop accommodating area; the liquid drop accommodating area comprises one or more culture modules, and the culture modules are provided with a plurality of culture holes; through holes are formed in the side walls, adjacent to each other, of the culture holes, and the specification of each through hole is smaller than that of an embryo in each culture hole.

The embryo culture dish is characterized in that the culture module is provided with four culture holes which are arranged in a matrix.

The embryo culture dish is characterized in that the culture module is provided with four culture holes, the culture holes are arranged in a matrix mode, and two culture holes are distributed in each row.

The embryo culture dish, wherein, the lateral wall is the screen cloth, the through-hole is the quad slit.

The embryo culture dish is characterized in that the length and the width of the square hole are both less than 100 mu m.

The embryo culture dish is characterized in that the bottom of the culture hole is a square with the side length of 0.5cm and the height of 0.3 cm.

The embryo culture dish is characterized in that the culture dish body is provided with two culture modules which are tightly connected.

The embryo culture dish is a cuboid.

Has the advantages that:

the utility model provides an embryo culture dish which is provided with a liquid drop accommodating area, wherein at least two culture modules are distributed in the liquid drop accommodating area, at least two communicated culture holes are formed in the culture modules, the culture holes are used for culturing a single embryo, and culture liquids in different culture holes can flow mutually through the through holes, so that the embryo collective culture can be realized, the single embryo can be traced and scored, and the embryo with higher potential can be selected; besides, the arrangement of the liquid drop accommodating area can improve the liquid drop preparation efficiency and facilitate the identification and marking of the liquid drops.

Drawings

FIG. 1 is a schematic structural diagram of an embryo culture dish according to the present invention.

Description of the main element symbols: culture dish lid 1, culture dish body 2, culture module 21, culture hole 211, lateral wall 212.

Detailed Description

The utility model provides an embryo culture dish, and in order to make the purpose, technical scheme and effect of the utility model clearer and clearer, the utility model is further described in detail by referring to the attached drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

Referring to FIG. 1, the present invention provides an embryo culture dish.

The embryo culture dish comprises a culture dish body 2 and a culture dish cover 1; the culture dish cover 1 is connected to the culture dish body 2 in a covering mode; the culture dish body 2 is provided with a liquid drop accommodating area; the liquid drop accommodation area comprises one or more culture modules 21, and the culture modules 21 are provided with a plurality of culture holes 211; through holes are arranged on the adjacent side walls 212 among the plurality of culture holes 211, and the specification of the through holes is smaller than the size of embryos in the culture holes 211.

The embryo culture dish is not only suitable for co-culture of embryos in different development stages, but also suitable for other cells needing co-culture.

When the embryo culture dish is used for embryo culture, single day1 embryo can be respectively placed in the culture hole 211, and when the embryo is cultured to day3, the culture solution suitable for the development of day3-day7 embryo is replaced. The side wall 212 can prevent the embryos from being close to each other and being adhered together, and the embryos are provided with the independent culture holes 211, so that a culture worker can continuously observe the development condition of the embryos in the blastocyst stage to acquire more beneficial data. The size of the through holes on the side wall 212 is controlled to allow only substances such as growth factors secreted by the culture medium and embryos to pass through, and the embryos cannot be transferred to other culture holes 211 through the through holes, so that the effect of embryo collection culture is realized, and the problems that the embryos cannot be distinguished, traced and individually scored in the past during collection culture are solved. The embryo culture dish can also be used for preparing culture liquid drops in advance, and compared with the method of independently preparing the culture liquid drops each time, the method has higher efficiency of preparing the culture liquid drops in advance in batches. Preferably, the embryo culture dish can be made of transparent materials, the change and the state of the color of the culture solution can be observed from the outside, whether the culture solution needs to be replaced or is polluted or not is judged, the culture dish cover 1 does not need to be uncovered when the embryo state is observed by using a microscope, and the pollution risk is reduced.

Four culture holes 211 are arranged in the culture module 21, and the culture holes 211 are arranged in a matrix, preferably, two culture holes are arranged in each row. Each of the culture wells 211 has two sides adjacent to the other culture wells 211 to increase the circulation of the culture solution. Since only a thin sidewall 212 is formed between the culture wells 211, the growth factors, hormone factors, etc. secreted from the embryos in the culture wells 211 can be rapidly diffused into the culture wells 211 at the opposite corners. In the current research, one droplet (80-100 μ L) is used for culturing 4-5 day3 embryos in a collective culture mode, and the optimal blastocyst formation rate, pregnancy rate and the like are achieved.

Preferably, the sidewall 212 is a screen, and the through holes are square holes. A plurality of through holes are uniformly distributed on the screen mesh, so that various nutrient components in the culture solution and substances such as growth factors secreted by embryos can be fully circulated; adopt the quad slit can arrange on the screen cloth inseparabler, and the shared area in interval between through-hole and the through-hole is minimum, can have bigger flow area.

Further, the length and the width of the square hole are both smaller than 100 μm. The average diameter of the ovum is about 100-150 μm, therefore, the length and width of the opening of the square hole are both less than 100 μm, which can avoid the ovum passing through, and the ovum with the diameter less than 100 μm in the culture can be generally considered as abnormal ovum and eliminated. Preferably, the square holes are square, the side length is 80 μm, the larger the opening of the through hole is, the smaller the number of the through holes arranged on the screen is, the smaller the occupied area between the through holes is, and the larger the corresponding area for the culture solution to flow through is.

Preferably, the bottom of the culture well 211 is a square with a side of 0.5cm and a height of 0.3 cm. In the process of aggregate culture, 20-30 μ L of culture solution needs to be added into each culture hole 211, and the total amount of the culture solution needed to be added corresponding to one culture module can be calculated according to the total number of the culture holes 211, namely the amount needed for making one droplet. In this example, each culture well 211 had a capacity of 75. mu.L and a bottom area of 0.25cm²After the culture solution is added, the depth of the liquid drop is proper, the bottom area of the culture hole 211 cannot be too small, so that the insertion operation of a suction tube, a gun head and the like is facilitated, the bottom area of the culture hole 211 cannot be too large, and the embryo in the culture hole 211 can be easily and quickly found by observing under a microscope.

A plurality of liquid drops can be prepared in one culture dish body 2, in the embodiment, two culture modules 21 are arranged in the middle of the culture dish body 2, and the two culture modules 21 are tightly connected. In the case of collective culture, a plurality of embryos meeting the requirements can be divided into two groups for culture, and the embryos with more potential can be selected. The two culture modules 21 are tightly connected, so that the visual field can be changed as soon as possible during the observation by a microscope, and the efficiency is improved. When a contrast experiment is carried out, the control group and the experimental group can be observed and photographed under the same visual field, and the difference between the control group and the experimental group can be seen more intuitively.

Preferably, the embryo culture dish is a cuboid. Adopt the cuboid design, better hold between the fingers when external manual operation, when observing under the microscope, can be better fix on the objective table. When putting, the setting of cuboid can be more abundant utilize the space, puts more neatly.

It should be understood that the technical solutions and the inventive concepts according to the present invention may be equally replaced or changed by those skilled in the art, and all such changes or substitutions should fall within the protection scope of the appended claims.

Claims

1. An embryo culture dish is characterized by comprising a culture dish body and a culture dish cover; the culture dish cover is connected to the culture dish body in a covering mode; a liquid drop accommodating area is arranged in the culture dish body; the liquid drop accommodating area comprises one or more culture modules, and the culture modules are provided with a plurality of culture holes; through holes are formed in the side walls, adjacent to each other, of the culture holes, and the specification of each through hole is smaller than that of an embryo in each culture hole.

2. An embryo culture dish according to claim 1, wherein the culture module is provided with four culture wells, and the culture wells are arranged in a matrix.

3. An embryo culture dish according to claim 2, wherein the culture module is provided with four culture wells, the culture wells being arranged in a matrix, two in each row.

4. The embryo culture dish according to claim 1, wherein the side wall is a screen and the through hole is a square hole.

5. An embryo culture dish according to claim 4, wherein the square hole has a length and width of less than 100 μm.

6. An embryo culture dish according to claim 1, wherein the bottom of the culture well is a square with a side of 0.5cm and a height of 0.3 cm.

7. An embryo culture dish according to claim 1 wherein the body of the culture dish is provided with two culture modules which are closely connected.

8. The embryo culture dish according to claim 1, wherein the embryo culture dish is a rectangular parallelepiped.