CN202744549U - Automatic solution replacing embryo culture dish - Google Patents
Automatic solution replacing embryo culture dish Download PDFInfo
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- CN202744549U CN202744549U CN 201220238021 CN201220238021U CN202744549U CN 202744549 U CN202744549 U CN 202744549U CN 201220238021 CN201220238021 CN 201220238021 CN 201220238021 U CN201220238021 U CN 201220238021U CN 202744549 U CN202744549 U CN 202744549U
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- embryo
- district
- embryo culture
- culture
- waste liquid
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Abstract
The utility model discloses an automatic solution replacing embryo culture dish which comprises a dish body and a dish cover sealing the opening of the dish body, and is characterized in that an embryo culture chamber is mounted on the inner side wall of the dish body in a suspended manner; the interior of the embryo culture chamber is divided into an embryo culture area and a waste solution discharge area; an overflow passage is arranged between the embryo culture area and the waste solution discharge area; the bottom of the overflow passage is higher than those of the embryo culture area and the waste solution discharge area; a through hole, through which external culture solution can be dripped into the embryo culture area for supplementation, is formed on the dish cover; and when the solution level of culture solution in the embryo culture area is higher than the bottom of the overflow passage, culture solution in the embryo culture area can flow to the waste solution discharge area through the overflow passage. The automatic solution replacing embryo culture dish can be matched with an automatic solution dripping pump, so as to provide embryo culture solution and replace waste solution at the same time automatically and quantificationally, and further provide nutrients required for embryonic development, and reduce harm caused by repeated moving for solution replacement during the embryo culture process.
Description
Technical field
The utility model relates to a kind of medical instruments, relates in particular to a kind of embryo from moving the liquid culture dish.
Background technology
Vitro culture is a key link in IVF (IVFET) technology, and the external environment of embryo growth directly affects growing of body early embryo, and then has influence on embryo's plantation success ratio.Embryo's vitro culture is the process of a complexity, and it all has strict requirement to the many factors in the growing environment such as Air quality, temperature, nutrient solution composition and pH value and light etc.Whether tube baby's success or not is depended on to a great extent can have a stable culture systems, and when stable culture environment has been arranged, the embryo can healthy growth and development, relatively improves embryo's availability.And in this stable system, embryo medium is being played the part of very important role, because it provides the embryo to divide needed nutrient when growing.Be rich in the embryo medium for needed each seed amino acid of fetal development, but the embryo is its physiology and constantly variation of metabolism generation in culturing process, therefore the ammonia that amino acid decomposes and embryo metabolism produces need the successional embryo medium that provides also to replace simultaneously waste liquid to the toxic effect of embryo.
Changing at present embryo medium does not have special method, and the simple exactly artificial imbibition device that passes through sucks nutrient solution.Each culture dish need to being taken out from incubator when changing nutrient solution just can be carried out afterwards, and such liquid process of changing has been destroyed stable culture systems virtually, and the embryo in the culturing process has been caused certain injury.
Summary of the invention
The purpose of this utility model is the defective that overcomes prior art, provide a kind of embryo from moving the liquid culture dish, the ware lid that comprises ware body and sealing ware body open-mouth, it is characterized in that: the outstanding embryo culture chamber of being equipped with of described ware body inner side-wall, indoor embryo culture district and the discharging of waste liquid district of being separated into of this embryo culture, be provided with overflow ducts between described embryo culture district and the discharging of waste liquid district, the bottom of this overflow ducts is higher than the bottom in described embryo culture district and discharging of waste liquid district;
Described ware lid is provided with for the external fresh medium of ware and replenishes the through hole that splashes into described embryo culture district, when the liquid level of nutrient solution in the described embryo culture district was higher than described overflow ducts bottom, the nutrient solution in the described embryo culture district flowed into described discharging of waste liquid district through overflow ducts automatically.
The sidewall in described discharging of waste liquid district is provided with and prevents waste reflux to the waste liquid flow pass in described embryo culture district, and the bottom of this waste liquid flow pass is lower than the bottom of described overflow ducts.
Baffle for separating in the middle of described embryo culture chamber is arranged on becomes described embryo culture district and discharging of waste liquid district, and the height of described dividing plate is lower than the height of other sidewall of described embryo culture chamber, but is higher than the height of the short dividing plate of described discharging of waste liquid district one side; The above space of described dividing plate apical margin consists of described overflow ducts, and the above space of described short dividing plate apical margin consists of the waste liquid flow pass in described discharging of waste liquid district.
The external liquid changing device liquid output syringe needle activity of the through hole of described ware lid and ware pegs graft to replenish at regular time and quantity fresh medium to described embryo culture district.
The outstanding plural embryo culture chamber of being equipped with of described ware body inner side-wall.
Be provided with T shape draw-in groove on the described ware body inner side-wall, the outdoor sidewall of described embryo culture convex with can with the movable T shape card key of pegging graft of described T shape draw-in groove.
Described embryo culture chamber is take described dividing plate as a sidewall, and it highly is 3/4ths of other face sidewall height, and described discharging of waste liquid district is take described short dividing plate as a sidewall, and it highly is lower than the height of described dividing plate.
Described ware lid inner-wall surface is to downward-extension one right cylinder, and described through hole runs through this right cylinder center; Described right cylinder both sides are extended with the lug for stationary cylinder.
Described embryo culture chamber is rectangular shape.
Compared with prior art, the beneficial effects of the utility model are: needed nutrient in the embryo development procedure is provided, reduce the embryo in culturing process because the injury that mobile replacing nutrient solution repeatedly causes, the physiology machine that the embryo can be met in the body turns, improve the rate of forming of blastaea, also increased clinical pregnancy rate simultaneously.
Description of drawings
With reference to accompanying drawing, the utility model will be better understood.Accompanying drawing is as follows:
Fig. 1 be the utility model embryo from move the liquid culture dish vertical view;
Fig. 2 be the utility model embryo from move the liquid culture dish along its A-A line sectional view;
Fig. 3 is that the utility model embryo is from moving the vertical view of embryo culture chamber in the liquid culture dish;
Fig. 4 be the utility model embryo from move liquid culture dish ware body along its B-B line sectional view;
Fig. 5 is that the utility model embryo is from the vertical view of moving the another kind of embodiment of liquid culture dish.
Embodiment
In order to make the purpose of this utility model, technical scheme, beneficial effect clearer, below in conjunction with drawings and Examples, the utility model is described in further details.Should be appreciated that specific embodiment described herein only is used for explaining the utility model, and be not used in restriction the utility model.
Below in conjunction with specific embodiment realization of the present utility model is described in detail:
As depicted in figs. 1 and 2, a kind of embryo comprises ware lid 2 and the embryo culture chamber 11 of ware body 1, sealing ware body open-mouth from moving the liquid culture dish.Embryo culture chamber 11 is divided into embryo culture district 112 and discharging of waste liquid district 113.Embryo culture chamber 11 is rectangular shape, is appreciated that the embryo culture chamber also can adopt other shapes, such as the right cylinder that links to each other etc.Be provided with overflow ducts 115 between embryo culture district 112 and the discharging of waste liquid district 113, the bottom of this overflow ducts 115 is higher than the bottom in described embryo culture district 112 and discharging of waste liquid district 113.Overflow ducts 115 can be a circle or the passage of other shapes, also can be to form overflow ducts by the above space of dividing plate apical margin, is not limited to these embodiments, and the structure that can reach same effect all can.
Concrete, the dividing plate 111 in the middle of this embryo culture chamber 11 is arranged on is divided into embryo culture district 112 and discharging of waste liquid district 113, and discharging of waste liquid district 113 1 sides are provided with short dividing plate 114.The height of dividing plate 111 is lower than the height of embryo culture chamber 11 other sidewalls, but is higher than the height of the short dividing plate 114 of described discharging of waste liquid district 113 1 sides.Embryo culture district 112 is take described dividing plate 111 as a sidewall, and preferred, it highly is 3/4ths of other face sidewall height, and discharging of waste liquid district 113 is take described short dividing plate 114 as a sidewall, and it highly is lower than the height of described dividing plate 111.The above space of dividing plate 111 apical margins consists of overflow ducts 115, and the bottom of this overflow ducts 115 is higher than the bottom in embryo culture district 112 and discharging of waste liquid district 113.Formation discharging of waste liquid district, the above space of short dividing plate 114 apical margins 113 can prevent waste reflux to the waste liquid flow pass 116 in described embryo culture district 112, and the bottom of this waste liquid flow pass 116 is lower than the bottom of described overflow ducts 115.Described waste liquid flow pass 116 also can adopt other structures with identical function, has the passage of definite shape etc. such as the employing cross section.
Ware covers 2 inner-wall surfaces to downward-extension one right cylinder 22, and right cylinder 22 both sides are extended with the lug 23 for stationary cylinder.Ware lid 2 is provided with for the external fresh medium of ware and replenishes the through hole 21 that splashes into embryo culture district 112, and through hole 21 runs through the central position of right cylinder 22.The external quantitative automatic drip pump output syringe needle (not shown in the accompanying drawing) of the through hole 21 of ware lid 2 and ware is movable pegs graft 112 to replenish fresh mediums to the embryo culture district at regular time and quantity.When the liquid level of embryo culture district 112 interior nutrient solutions was higher than overflow ducts 115 bottom, the nutrient solution in the described embryo culture district 112 flowed into discharging of waste liquid district 113 through overflow ducts 115 automatically.
To change the process of enchylema as follows from moving the liquid culture dish to utilize the embryo:
The embryo that needs are cultivated is placed in the embryo culture district 112 that fills nutrient solution, and at this moment, the liquid level of embryo culture district 112 interior nutrient solutions is lower than the height of dividing plate 111.The embryo is covered ware lid 2 from moving the liquid culture dish, and the output syringe needle (not shown in the accompanying drawing) that will be connected with quantitative automatic drip pump one end of nutrient solution inserts in the through hole 21 on the ware lid 2, by the automatic drip pump of adjustment quantitative so that the output syringe needle 112 interiorly replenishes fresh nutrient solutions to the embryo culture district at regular time and quantity.
By constantly splashing into fresh nutrient solution, the nutrient solution in the embryo culture district 112 constantly increases.When accumulating to a certain amount of the time, the nutrient solution in embryo culture district 112 automatically overflows across dividing plate 111 and flow in the discharging of waste liquid district 113 that is adjacent.
Under normal circumstances, the complete waste liquid of splendid attire embryo culture district 112 discharging in discharging of waste liquid district 113.But under the abnormal conditions, such as situations such as quantitative automatic drip pump break down, waste liquid can overflow across waste liquid flow pass 116 and flow in the ware bodies 1, avoid waste reflux to the embryo culture district 112 interior infringement embryos.
Fig. 5 is that the utility model embryo is from the another kind of embodiment of moving the liquid culture dish, this embryo is basic identical from moving liquid culture dish structure to embryo shown in Figure 4 from moving liquid culture dish and Fig. 1, difference is that ware body 1 inner side-wall hangs two embryo culture chambers 11 are housed.Just schematically show two embryo culture chambers 11 among the figure, in fact can hang and fill plural embryo culture chamber 11.Such benefit is, can be placed on an embryo to a plurality of embryos of a couple who cultivates and cultivate from moving in the liquid culture dish, avoids confusion.
The above only is preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of within spirit of the present utility model and principle, doing, be equal to and replace and improvement etc., all should be included within the protection domain of the present utility model.
Claims (9)
1. an embryo is from moving the liquid culture dish, the ware lid (2) that comprises ware body (1) and sealing ware body open-mouth, it is characterized in that: the outstanding embryo culture chamber (11) of being equipped with of described ware body (1) inner side-wall, be separated into embryo culture district (112) and discharging of waste liquid district (113) in this embryo culture chamber (11), be provided with overflow ducts (115) between described embryo culture district (112) and discharging of waste liquid district (113), the bottom of this overflow ducts (115) is higher than the bottom of described embryo culture district (112) and discharging of waste liquid district (113);
Described ware lid (2) is provided with for the external fresh medium of ware and replenishes the through hole (21) that splashes into described embryo culture district (112), when the liquid level of nutrient solution in the described embryo culture district (112) was higher than described overflow ducts (115) bottom, the nutrient solution in the described embryo culture district (112) flowed into described discharging of waste liquid district (113) through overflow ducts (115) automatically.
2. embryo as claimed in claim 1 is from moving the liquid culture dish, it is characterized in that: the sidewall in described discharging of waste liquid district (113) is provided with and prevents waste reflux to the waste liquid flow pass (116) in described embryo culture district (112), and the bottom of this waste liquid flow pass (116) is lower than the bottom of described overflow ducts (115).
3. embryo as claimed in claim 2 is from moving the liquid culture dish, it is characterized in that: the dividing plate (111) in the middle of described embryo culture chamber (11) is arranged on is separated into described embryo culture district (112) and discharging of waste liquid district (113), the height of described dividing plate (111) is lower than the height of other sidewall of described embryo culture chamber (11), but is higher than the height of the short dividing plate (114) of described discharging of waste liquid district (113) one sides; The above space of described dividing plate (111) apical margin consists of described overflow ducts (115), and the above space of described short dividing plate (114) apical margin consists of the waste liquid flow pass (116) in described discharging of waste liquid district (113).
4. embryo as claimed in claim 3 is characterized in that from moving the liquid culture dish: the external liquid changing device liquid output syringe needle activity of the through hole (21) of described ware lid (2) and ware pegs graft to replenish at regular time and quantity fresh medium to described embryo culture district (112).
5. certainly move the liquid culture dish such as the described embryo of each claim of claim 1 to 4, it is characterized in that: the outstanding plural embryo culture chamber (11) of being equipped with of described ware body (1) inner side-wall.
6. certainly move the liquid culture dish such as the described embryo of each claim of claim 1 to 4, it is characterized in that: be provided with T shape draw-in groove (12) on described ware body (1) inner side-wall, described embryo culture chamber (11) outer side wall convex with can with the movable T shape card key (117) of pegging graft of described T shape draw-in groove (12).
7. embryo as claimed in claim 3 is from moving the liquid culture dish, it is characterized in that: described embryo culture district (112) is take described dividing plate (111) as a sidewall, it highly is 3/4ths of other face sidewall height, described discharging of waste liquid district (113) is take described short dividing plate (114) as a sidewall, and it highly is lower than the height of described dividing plate (111).
8. certainly move the liquid culture dish such as the described embryo of each claim of claim 1 to 4, it is characterized in that: described ware lid (2) inner-wall surface is to downward-extension one right cylinder (22), and described through hole (21) runs through this right cylinder (22) central position; Described right cylinder (22) both sides are extended with the lug (23) for stationary cylinder (22).
9. certainly move the liquid culture dish such as the described embryo of each claim of claim 1 to 4, it is characterized in that: described embryo culture chamber (11) is rectangular shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220238021 CN202744549U (en) | 2012-05-25 | 2012-05-25 | Automatic solution replacing embryo culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220238021 CN202744549U (en) | 2012-05-25 | 2012-05-25 | Automatic solution replacing embryo culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202744549U true CN202744549U (en) | 2013-02-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220238021 Expired - Lifetime CN202744549U (en) | 2012-05-25 | 2012-05-25 | Automatic solution replacing embryo culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202744549U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421682A (en) * | 2012-05-25 | 2013-12-04 | 曾勇 | Automatic liquid changing culture dish for embryo |
| CN103505299B (en) * | 2013-09-26 | 2015-08-12 | 四川大学 | Oral cavity in-site dental plaque collection device |
| CN110093273A (en) * | 2019-06-13 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Culture module is imaged in embryo's time difference |
-
2012
- 2012-05-25 CN CN 201220238021 patent/CN202744549U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421682A (en) * | 2012-05-25 | 2013-12-04 | 曾勇 | Automatic liquid changing culture dish for embryo |
| CN103421682B (en) * | 2012-05-25 | 2014-12-10 | 曾勇 | Automatic liquid changing culture dish for embryo |
| CN103505299B (en) * | 2013-09-26 | 2015-08-12 | 四川大学 | Oral cavity in-site dental plaque collection device |
| CN110093273A (en) * | 2019-06-13 | 2019-08-06 | 中国科学院苏州生物医学工程技术研究所 | Culture module is imaged in embryo's time difference |
| CN110093273B (en) * | 2019-06-13 | 2023-12-05 | 济南国科医工科技发展有限公司 | Embryo time difference imaging culture module |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: ZHOU CANQUAN CAI JING CHEN WEIBIN Effective date: 20130425 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130425 Address after: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Patentee after: Zeng Yong Patentee after: Zhou Canquan Patentee after: Cai Jing Patentee after: Chen Weibin Address before: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Patentee before: Zeng Yong |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20130220 Effective date of abandoning: 20141210 |
|
| RGAV | Abandon patent right to avoid regrant |