CN110093273A - Culture module is imaged in embryo's time difference - Google Patents
Culture module is imaged in embryo's time difference Download PDFInfo
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- CN110093273A CN110093273A CN201910511314.0A CN201910511314A CN110093273A CN 110093273 A CN110093273 A CN 110093273A CN 201910511314 A CN201910511314 A CN 201910511314A CN 110093273 A CN110093273 A CN 110093273A
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 81
- 238000012258 culturing Methods 0.000 claims abstract description 84
- 235000014676 Phragmites communis Nutrition 0.000 claims description 25
- 238000012549 training Methods 0.000 claims description 17
- 238000005192 partition Methods 0.000 claims description 11
- 230000007246 mechanism Effects 0.000 claims description 9
- 230000005693 optoelectronics Effects 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 5
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- 239000000243 solution Substances 0.000 description 16
- 238000003384 imaging method Methods 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- 238000010586 diagram Methods 0.000 description 11
- 239000000523 sample Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
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- 238000013461 design Methods 0.000 description 5
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- 238000001139 pH measurement Methods 0.000 description 4
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- 238000009826 distribution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 238000012544 monitoring process Methods 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000013316 zoning Methods 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 241000976806 Genea <ascomycete fungus> Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 230000007812 deficiency Effects 0.000 description 1
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- 230000013020 embryo development Effects 0.000 description 1
- 210000003811 finger Anatomy 0.000 description 1
- 239000008246 gaseous mixture Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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Abstract
The invention discloses a kind of embryo's time differences to be imaged culture module, comprising: culturing room's ontology and is set to the intrinsic culture dish of culturing room；It include at least two culture ponds in culture dish, culture pond includes top pond body and lower part pond body, and the inner wall of top pond body is connected with the inner wall of lower part pond body, and the inclination angle of the inner wall of top pond body is less than the inclination angle of the inner wall of lower part pond body.The present invention is by being arranged more than two culture ponds, and user can distribute according to the reasonable quantity of culture embryo, to avoid the waste of unnecessary culture solution；Culture pond upper inside wall opposing lower portions inner wall is in obvious angle, there is apparent boundary characteristic, is easier to recognize and predict liquid level position during user's pipetting；Embryo's micropore includes upper hole and bottom outlet, is quickly positioned convenient for user to micropore area and embryo's importing, while being avoided that embryo undesirably falls off to portal and causing aliasing, more securely and reliably；Culture pond upper inside wall is designed in angle, reduces influence of the concave moon surface to optical imagery.
Description
Technical field
The present invention relates to biological sample Cultivating techniques field, in particular to culture module is imaged in a kind of embryo's time difference.
Background technique
With comprehensive relieving of national two child's policies, vitro fertilization-embryo implanting (IVF-ET) can treat as one
The Issues of Human Assisted Reproductive Technologies of infertility receives more concerns.However pregnancy rate it is lower be current assisted reproductive technology development
How one of bottleneck improves the availability of embryo, and obtaining higher Clinical Pregnancy Rate in becomes the research in supplementary reproduction field
Hot spot.
Embryo's time difference, which photographs, monitors culture systems, abbreviation time difference culture systems, is that the emerging clinical embryo that is applied to sieves
The characteristics of selecting evaluation method, no human interference training method and real time monitoring can carry out dynamic observation to embryo, utilize form
Kinetic parameter is evaluated, and the selection result is made more to objectify.Therefore time difference culture systems pass through time difference imaging technique, assessment
The potentiality of development of embryo filters out top quality embryo and transplants, to be effectively improved assisted reproductive technology clinical effectiveness, mentions
High implantation rate, Clinical Pregnancy Rate in shorten patient's successful pregnancies time.
Embryo's time difference, which is imaged in culture apparatus, generally can all be provided with Embryo Culture module, be formed in Embryo Culture module
There is a culturing room being avoided that, culture dish is imaged in setting embryo's time difference in culturing room, for carrying out Embryo Culture.
It is the mating dedicated embryo's carrier of time difference culture systems, generally plastics (such as polyester that culture dish, which is imaged, in embryo's time difference
Material) injection molding transparent configuration body, be made of ware body and ware lid, be placed in temperature, CO2 concentration by strict control culture
In environment.Multiple embryo's hole locations are generally arranged in culture dish bottom, and each hole location only accommodates single embryo, stores embryonic development in ware
Required culture solution.There is micro imaging system to clap at regular intervals embryo's image each in ware except culture environment
It takes the photograph and records, be fabricated to delay photograph album, for image algorithm identification and development evaluation.
Common embryo's time difference, which cultivates, independent culture and shared training method, exists wherein independent training method separates embryo
In individual culture pond, the common culture pond for having straight hole and taper hole form sinks to having micropore, be used in culture bottom of pond portion middle
Single piece of embryo is stored, all Embryo Culture ponds are linearly distributed or array distribution, according to actual needs due to this training method
Embryo's quantity of culture adds culture solution, relatively saves.Shared training method, i.e., all embryos are in the same culture pond
Interior, culture bottom of pond portion sinking has multiple micropores, and is distributed in certain geometrical characteristic, and embryo is stored in each micropore.Two
Kind training method is to store culture solution, top oil overlay (such as mineral oil), to inhibit culture solution to volatilize in culture pond lower part.
Shared training method is with the serial time difference culture device of the EmbryoScope of Unisense FertiliTech company
Mating culture dish is typical case, added no matter how high the utilization rate in Embryo Culture hole is since all embryos are in the same cultivation region
The culture solution dosage entered is all, it is easy to culture solution dosage be caused to waste with respect to embryo's quantity redundancy bring.Australia
The culture dish for embryo samples development monitoring that Genea Limited announces equally is easy that there are the above drawbacks, in addition,
Its embryo's micropore is shallower, and by the design constraint of optical system, adjacent cells distance is especially close, not only imports embryo extremely to user
The operation of micropore brings certain difficulty, and needs especially careful in culture dish transfer process, crosses ambassador to avoid shaking amplitude
Embryo is detached from micropore, obscures mutually.
In addition, the time difference imaging culture dish culture hole there is a problem of it is more universal, i.e., all due to culture dish and culture solution
It is the transparent body, culture hole depth is again shallower, and user is difficult to accurately identify liquid level position in liquid relief, and the repeatability of artificial liquid feeding is difficult
With control, causes the culture solution of partial embryonic on the low side or waste is overflowed.
In addition, present Embryo Culture module there is also the closing lid precision of cover opening-closing mechanism is low, causes image quality to reduce
The problems such as, it is difficult to meet the use demand of user.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of embryo's time difference
Imaging culture module.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: culture module, packet is imaged in a kind of embryo's time difference
It includes: culturing room's ontology and being set to the intrinsic culture dish of the culturing room；
It include at least two culture ponds in the culture dish, the culture pond includes top pond body and lower part pond body, top
The inner wall of pond body is connected with the inner wall of lower part pond body, and the inclination angle of the inner wall of the top pond body is less than the interior of the lower part pond body
The inclination angle of wall, wherein the inclination angle refers to the inner wall of the top pond body or lower part pond body and the angle of horizontal direction.
Preferably, the culture dish includes ware body and ware lid, and the ware body includes that rounded portions and width are less than the circle
The rectangular portion of the diameter in shape portion, the side of the rectangular portion are in the inside of the rounded portions, and the rounded portions are stretched out in the other side；
Cultivation region is provided on the ware body, the culture pond is arranged in the cultivation region；
The cultivation region by the bottom plate and the cultivation region coaming plate that is upwardly extended from the bottom plate of the ware body around being formed, institute
State the recessed formation culture pond of bottom plate, rinse bath and serial number mark.
The culture pond includes top pond body and lower part pond body, and the inner wall of top pond body is connected with the inner wall of lower part pond body,
And the inclination angle of the inner wall of the top pond body is less than the inclination angle of the inner wall of the lower part pond body, wherein the inclination angle refers on described
The angle of the inner wall and horizontal direction of portion's pond body or lower part pond body.
Preferably, the bottom of the lower part pond body is provided at least one sample micropore, and the sample micropore includes upper
Hole and bottom outlet, the internal diameter in the upper hole are greater than the internal diameter of the bottom outlet, and the inner wall of the bottom outlet and vertical direction be not with being
0 ° of angle.
Preferably, the ware lid is for being fastened on the ware body comprising cover board and the ware extended downwardly from cover board
Lid coaming plate, when the ware lid is fastened on the ware body, the cultivation region coaming plate of the ware body is in the inside of the ware lid coaming plate.
Preferably, the ware lid further includes that hand-held piece and partition, the partition extend out from ware lid bottom surface, and described
Cover board is parallel, and the hand-held piece is vertically set on the partition.
Preferably, it is provided with boss at four vertex positions of the cover board lower wall, the ware lid is fastened on the ware
When on body, the cover board is supported on the cultivation region coaming plate by four boss.
Preferably, PH measurement zone, trade mark area, label area and holding area are additionally provided on the ware body.
Preferably, culturing room's ontology includes culturing room's platform and culturing room's upper cover, and the two constitutes closed sample
Culturing room；The ware slot for the culture dish to be arranged, the shape and the training of the ware slot are offered on culturing room's platform
The bottom shape for supporting ware matches, and the light tank for penetrating through culturing room's platform is offered among the ware slot.
Preferably, the rear end of culturing room's upper cover is connect by rotary gemel with culturing room's platform, the training
The bottom surface for supporting room upper cover is provided with annular groove, is filled with sealing strip in the annular groove, the front end of culturing room's ontology is also set up
There is open-and-close mechanism；
The open-and-close mechanism includes the baffle being arranged in the culturing room on lid, unlocking board, hinge axis, hinge seat, the
Two tension springs, latch hook, locating piece and the optoelectronic switch being arranged on culturing room's platform, reed, reed briquetting；
The latch hook is integrally L-shaped, is from top to bottom disposed with hinge hole, tension spring hole and hook along its length thereon
Head, the bottom of the gib head end are equipped with bevelling, and the latch hook is cooperated by hinge hole and hinge axis, is rotatably provided in affixed
In on the hinge seat of culturing room's upper cover；
One end of second tension spring is connect with tension spring hole, the other end and the column company being mounted in the culturing room on lid
It connects；
The locating piece is fixed on culturing room's upper cover bottom, is hollow structure, and the latch hook from top to bottom passes through described
The gib head of locating piece, the latch hook bottom exposes the locating piece.
Preferably, offer rectangular opening on culturing room's platform below the latch hook, on the rectangular opening with
Bevel edge is provided on the corresponding side wall of the bevelling of the gib head, the bevel edge bottom hollows out to form hook groove；
The reed is set to below the rectangular opening, L-shaped, and the short side of the reed is in below the gib head,
Long side one end of the reed is pushed down by reed briquetting, and the optoelectronic switch is set to the lower section of the short side of the reed；
It is additionally provided on the locating piece for cooperating the convex block being inserted into the rectangular opening, the two sides of the convex block are set
It is equipped with for the positioning straight flange with the cooperation of two inner vertical walls of the rectangular opening, it is described after the convex block plugs the rectangular opening
Positioning straight flange is in the bevel edge two sides of the rectangular opening.
Preferably, the bottom surface of culturing room's upper cover is provided with spring catch, and the spring catch includes guide, pin and pressure
Spring, the guide are cylinder, inside open up step circular hole, step circular hole include the great circle hole section from top to bottom set gradually and
Roundlet hole section；The lower end of the pin is bulb, is slidably arranged in the roundlet hole section, the upper end of the pin is provided with diameter
Greater than the rotary table rank of the roundlet hole section, the rotary table rank is slidably arranged in the great circle hole section；The pressure spring setting exists
In the great circle hole section, and lower end presses against on the rotary table rank；
The taper hole for the bulb cooperation insertion for the pin is offered on culturing room's platform, the taper hole and pin are total
Axis.
The beneficial effects of the present invention are: embryo's time difference of the invention is imaged in culture module, pass through the more than two trainings of setting
Pond is supported, user can distribute according to the reasonable quantity of culture embryo, to avoid the waste of unnecessary culture solution；In the present invention, training
Supporting pond upper inside wall opposing lower portions inner wall is in obvious angle, has apparent boundary characteristic, is easier during user's pipetting
Identification and prediction liquid level position；In the present invention, embryo's micropore includes upper hole and bottom outlet, quickly positioned convenient for user to micropore area and
Embryo imports, while being avoided that embryo undesirably falls off to portal and causing aliasing, more securely and reliably；In the present invention, culture pond
Upper inside wall is designed in angle, reduces influence of the concave moon surface to optical imagery.In the present invention, by being arranged on culturing room's ontology
Open-and-close mechanism makes capping position precision height in culturing room's ontology, can ensure that the reliably working of micro imaging system.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for embryo's time difference of the invention culture module being imaged；
Fig. 2 is the structural schematic diagram of culture dish of the invention；
Fig. 3 is the function zoning schematic diagram of ware body of the invention；
Fig. 4 is the structural schematic diagram of ware body of the invention；
Fig. 5 is the structural schematic diagram of ware lid of the invention；
Fig. 6 is the bottom substance schematic diagram of ware lid of the invention；
Fig. 7 is the schematic cross-sectional view of culture dish of the invention；
Fig. 8 is the structural schematic diagram of ware lid and the cooperation of ware body of the invention；
Fig. 9 is schematic diagram when culture dish of the invention uses；
Figure 10 is that culture module application is imaged when a specific embryo in embryo's time difference of the invention in a kind of embodiment
The structural schematic diagram of poor culture apparatus；
Figure 11 is the schematic cross-sectional view of culturing room's ontology of the invention；
Figure 12 is the partial enlargement structural representation in Figure 11 of the invention at A；
Figure 13 is the partial enlargement structural representation in Figure 11 of the invention at B；
Figure 14 is the structural schematic diagram of locating piece of the invention；
Figure 15 is the structural schematic diagram of rectangular opening of the invention.
Description of symbols:
1-culturing room's ontology；2-culture dishes；3-ware bodies；4-ware lids；5-culture ponds；6-rinse baths；7-serial number marks
Show；20-cultivation regions；21-PH measurement zones；22-trade mark areas；23-label areas；24-holding areas；30-rounded portions；31-squares
Shape portion；32-bottom plates；33-cultivation region coaming plates；40-cover boards；41-ware lid coaming plates；42-hand-held pieces；43-partitions；44-is convex
Platform；50-top pond bodies；51-lower part pond bodies；52-sample micropores；53-upper holes；54-bottom outlets；55-overwrite medias；56—
Culture solution；
10-culturing room's platforms；11-culturing room's upper covers；12-heating elements, temperature sensor；13-insulating layers；14—
Air inlet；15-gas outlets；16-ware slots；17-hinges；100-rectangular openings；101-bevel edges；102-hook grooves；103-cones
Hole；110-sealing strips；111-columns；112-spring catch；113-pins；114-guidees；115-pressure springs；116-step circles
Hole；
8-open-and-close mechanisms；80-baffles；800-ribs；81-unlocking boards；82-hinge axis；83-hinge seats；84—
Second tension spring；85-latch hooks；86-locating pieces；87-optoelectronic switches；88-reeds；89-reed briquettings；850-gib heads；
851-bevellings；860-convex blocks；861-positioning straight flanges；
9-embryo's time difference culture apparatus；90-light sources；91-micro imaging systems；
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
As shown in figures 1-15, culture module is imaged in a kind of embryo's time difference of the present embodiment, comprising: culturing room's ontology 1 and sets
The culture dish 2 being placed in culturing room's ontology 1；
Culture dish 2 includes ware body 3 and ware lid 4, and ware body 3 includes the square of rounded portions 30 and width less than the diameter of rounded portions 30
Shape portion 31, the side of rectangular portion 31 are in the inside of rounded portions 30, and rounded portions 30 are stretched out in the other side.The general shape of ware body 3 by
Round and rectangle boolean sums, and by the broadside of ware body 3 outer circle and rectangle, cooperates pair of the ware slot 16 in culturing room's ontology 1
It answers feature that can uniquely determine placement location and angle, reduces the monitoring error in Embryo Culture hole.
By function zoning, referring to Fig. 3, cultivation region 20, PH measurement zone 21, trade mark area 22, label area are provided on ware body 3
23 and holding area 24.Cultivation region 20 is used to embryo operation and culture；PH measurement zone 21 stores embryo medium 56, is supervised by measurement
It controls culture environment and situation is changed to the PH of culture solution 56；22 customizable products logo of trade mark area, name of product etc.；Label area
23 need binding mark to have the label of patient information or the bar code of machine readable format, two dimensional code etc. according to user；3 hand of ware body
Holding area 24 allows user to shift and pick and place culture dish 2 by way of thumb and index finger grip.In a kind of preferred embodiment,
3 outer circle outer diameter of ware body is 55mm, and 3 holding area of ware body, 24 width is 32mm, and for 24 end face of holding area apart from center of circle 36mm, ware body 3 is high
Degree is 10mm.
Wherein, cultivation region 20 by the bottom plate 32 and the cultivation region coaming plate 33 that is upwardly extended from bottom plate 32 of ware body 3 around being formed,
The recessed formation culture pond 5 of bottom plate 32, rinse bath 6 and serial number mark 7；Culture pond 5 is for storing embryo and culture solution 56, culture pond
Caching or embryo's cleaning when the rinse bath 6 that 5 sides are arranged is used to prepare culture solution 56 or change liquid operation, serial number mark 7
Embryo is encoded convenient for user and quick indexing.
Wherein, the cultivation region 20 of ware body 3 includes at least two culture ponds 5, in a kind of preferred embodiment, including 4
Culture pond 5, cultivation region 20 can at most accommodate 16 pieces of embryos, and 16 pieces of embryo distributions are being pressed 4 independent trainings arranged in a straight line
It supports in pond 5, each culture pond 5 accommodates 4 pieces of embryos, has the advantage that, before user can determine the use of according to embryo's quantity
Several culture ponds 5, it is possible to reduce all embryos share the waste in training method to culture solution 56.For example, embryo's quantity be 9~
When 12, it is only necessary to preceding 3 culture ponds 5 are utilized, when embryo's quantity is 5~8, it is only necessary to utilize preceding 2 culture ponds 5.
Culture pond 5 includes top pond body 50 and lower part pond body 51, the inner wall of top pond body 50 and the inner wall of lower part pond body 51
It is connected, and the inclination angle of the inner wall of top pond body 50 is less than the inclination angle of the inner wall of lower part pond body 51, wherein inclination angle refers to top pond body 50
Or lower part pond body 51 inner wall and horizontal direction angle.Lower part pond body 51 is for storing embryo medium 56, top pond body 50
For covering volatilization prevention medium, wherein upper inside wall is connected with lower inner wall, and is in obvious angle relative to lower inner wall.One
In kind of preferred embodiment, 5 total depth of culture pond about 4mm, lower depth 3mm, top depth 1mm, upper and lower inner wall in angle about
50 degree, each culture pond 5 includes 4 pieces of embryos, can accommodate about 150~160 microlitres of 56 volume of culture solution.
Since 5 top and the bottom of culture pond are in obvious angle, the boundary of top and the bottom is than more visible, during user's pipetting
It is easy to recognize and predict that liquid level is made a response at a distance from boundary, and ahead of time.
Normally due to the influence of contact surface intermolecular force and surface tension of liquid, liquid will form with hole wall contact position
Apparent concave moon surface causes potentially to influence on optical imagery, such as when straight hole training method.The influence of concave surface is avoided, it can be with
By increasing 5 space of culture pond, make method of the embryo far from inner wall, but the overall dimension that will cause ware body 3 becomes larger, space benefit
It is low with rate.5 upper inside wall of culture pond is connected with lower inner wall in the present embodiment, and is in obvious angle relative to lower inner wall, separately
One beneficial effect is presence of the concave moon surface due to inclination angle of overwrite media 55 and 5 upper inside wall of culture pond, concave moon surface
Radius becomes larger, and deviates outward, preferably avoids the irradiated region of incident light, improves imaging effect, and 3 structure of ware body is more
It is compact.
The bottom of lower part pond body 51 is provided at least one sample micropore 52, and sample micropore 52 includes upper hole 53 and bottom outlet
54, the internal diameter in upper hole 53 is greater than the internal diameter of bottom outlet 54, and the inner wall of bottom outlet 54 and vertical direction have the not angle for 0 °.Bottom outlet
54 inner walls and vertical direction are in obvious angle.In a kind of preferred embodiment, sample micropore 52 is equally spaced by the interval 2mm,
Wherein upper 53 internal diameter of hole is 1.6mm, and depth 0.6mm, 54 bottom diameter of bottom outlet is 0.4mm, depth 0.6mm, inner wall and vertical side
To being in about 15 degree of angle, each junction is rounding off.Since upper 53 internal diameter of hole is larger, embryo is being imported or is being removed bottom by user
It first can quickly be positioned at supreme hole 53 when hole 54, the angulation design of 54 inner wall of bottom outlet and vertical direction also functions to and preferably draws
Lead effect.In addition, if accidentally operation error, embryo is general, and the bottom outlet 54 that can only detach enters 53rd area Shang Kong, and be unlikely into
Enter public area, cause the phenomenon that obscuring mutually, more securely and reliably.
Ware lid 4 is for being fastened on ware body 3 comprising cover board 40 and the ware lid coaming plate 41 extended downwardly from cover board 40, ware
When lid 4 is fastened on ware body 3, the cultivation region coaming plate 33 of ware body 3 is in the inside of ware lid coaming plate 41.
Ware lid 4 further includes that hand-held piece 42 and partition 43, partition 43 extend out from 4 bottom surface of ware lid, hand parallel with cover board 40
Piece 42 is held to be vertically set on partition 43.The effect of partition 43 be in user's grip ware lid 4, be isolated hand to coaming plate inside
Potential microbiological contamination is possible, promotes safety.
It is provided with boss 44 at cover board 40 lower wall, four vertex positions, when ware lid 4 is fastened on ware body 3, cover board 40 is logical
Four boss 44 are crossed to be supported on cultivation region coaming plate 33.Ware lid 4 is lifted by boss 44 everywhere, guarantees 40 lower wall of cover board and training
There are gaps between feeding 33 roof of area's coaming plate, allow culture gas to enter cultivation region 20 and swap.
The transparent configuration body of plastics (such as polystyrene material, polyester material) injection molding can be used in culture dish 2, meets note
Be moulded into type technological principle, tranverse sectional thickness about 1mm of structure or so, different location can be thicker or thinner to realize part
Feature needs.
In one embodiment, referring to Fig.1, be provided in culturing room's platform 10 and culturing room's upper cover 11 heating element with
Temperature sensor, and controlled by independent temperature, the aluminum alloy material of culturing room's upper cover 11 and platform using high thermal conductivity coefficient, back
Face is covered each by insulating layer 13 to reduce the external thermal loss of structure.Even if the temperature control design by redundancy guarantees that side goes out
In the case where existing failure, the other side can be worked normally, and be unlikely to cultivate indoor environment and deteriorated in a short time, be stopped in advance
The enough time shifts culture dish 2.Air inlet 14 and gas outlet 15 are also offered respectively on culturing room's platform 10 and upper lid.For supplying
The disengaging of CO2, O2 and N2 gaseous mixture.
Cultivating module includes culturing room's platform and culturing room's upper cover, and the two constitutes closed sample culturing room；Culturing room is flat
The ware slot 16 for culture dish 2 to be arranged is offered on platform, the shape of ware slot 16 and the bottom shape of culture dish 2 match, and ware
The light tank of perforation culturing room's platform is offered among slot 16.
In one embodiment, the rear end of reference, 11-15, culturing room's upper cover 11 is flat by rotary gemel 17 and culturing room
Platform 10 connects, and the bottom surface of culturing room's upper cover 11 is provided with annular groove, is filled with sealing strip 110 in annular groove, cultivates the front end of module 1
It is additionally provided with open-and-close mechanism 8.Rotary gemel 17 is selected as permanent torsional moment hinge 17, and upper cover can stop at an arbitrary position after starting.
Open-and-close mechanism 8 uses lever principle, including baffle 80, the unlocking board 81, hinge being arranged in culturing room's upper cover 11
Axis 82, hinge seat 83, tension spring, latch hook 85, locating piece 86 and the optoelectronic switch 87 being arranged on culturing room's platform 10, reed 88,
Reed briquetting 89；
Latch hook 85 is whole L-shaped, is from top to bottom disposed with 17 hole of hinge, tension spring hole and gib head along its length thereon
850, the bottom of 850 end of gib head is equipped with bevelling 851, and latch hook 85 is cooperated by 17 hole of hinge and hinge axis 82, is rotatably arranged
On the hinge seat 83 for being fixed in culturing room's upper cover 11；
One end of second tension spring 84 is connect with tension spring hole, and the other end connects with the column 111 being mounted in culturing room's upper cover 11
It connects；
Locating piece 86 is fixed on 11 bottom of culturing room's upper cover, is hollow structure, and latch hook 85 from top to bottom passes through locating piece
86, the gib head 850 of 85 bottom of latch hook exposes locating piece 86.Locating piece 86 is fixed on 11 bottom of culturing room's upper cover, due to tension spring
Effect, 85 bottom of latch hook default can touch 86 inner wall of locating piece.Unlocking board 81 is fixedly connected with hinge axis 82, along shown by arrow side
To when stirring unlocking board 81, tension spring tension can be overcome, rotate 85 around hinge axis 82 of latch hook.Baffle 80 is fixed on culturing room's upper cover
11 tops, baffle 80 are on one side rib 800 (for example, can be opened by the way that fluting 801 is arranged on baffle 80 close to unlocking board 81
The back of slot 801 forms baffle), by the size design of unlocking board 81, unlocking board 81 easy to accomplish of stirring withstands rib 800
In the case of, the inner wall less than locating piece 86 is touched in 85 bottom of latch hook, to protect latch hook 85, while passing through unlocking board 81 and rib 800
Power effect, it is easy to culturing room's upper cover 11 is started, the purpose uncapped is played.
It in above-described embodiment, is in below latch hook 85 on culturing room's platform 10 and offers rectangular opening 100, on rectangular opening 100
Bevel edge 101 is provided on side wall corresponding with the bevelling 851 of gib head 850,101 bottom of bevel edge hollows out to form hook groove 102；Closing lid
When, under 850 bevelling 851 of gib head and 472 bevel edge 101 of square hole guiding and under tension spring effect, it can make in the gib head 850 of latch hook 85
Side very successfully be embedded in hook groove 102, under the pretightning force of sealing strip 110, gib head 850 can reliably with groove face contact.
Wherein, reed 88 is set to 100 lower section of rectangular opening, L-shaped, and the short side of reed 88 is in 850 lower section of gib head, spring
Long side one end of piece 88 is pushed down by reed briquetting 89, and optoelectronic switch 87 is set to the lower section of the short side of reed 88.88 one end of reed
It is pushed down by reed briquetting 89, horizontality is kept when the other end does not stress, can be bent downwardly by when lower pressure.Latch hook 85 when closing lid
Gib head 850 be embedded in hook groove 102,850 back of gib head can withstand 88 end of reed, make compressive strain under reed 88, reed 88 it is short
Side can trigger optoelectronic switch 87, to allow system identification to the folding condition of culturing room.
It is additionally provided on locating piece 86 for cooperating the convex block 860 being inserted into rectangular opening 100, four angles of convex block 860
The processing of bevelling 851 is done, it is only straight in positioning of the two sides of convex block 860 setting for the cooperation of two inner vertical walls of rectangular opening 100
Side 861, after convex block 860 plugs rectangular opening 100, positioning straight flange 861 is in 101 two sides of bevel edge of rectangular opening 100.Determine in closing lid
Position straight flange 861 can cooperate with the inner wall of square hole 472, play culturing room's upper cover 11 with respect to culturing room's platform 10 in transverse direction
Positioning action.
After working long hours and wear due to the folding hinge 17 of culturing room's upper cover 11, oneself inter deterioration of folding hinge 17
There is gap, closing lid Hou Huishi culturing room upper cover 11 uncertainty occurs with respect to the position of culturing room's platform 10, makes 90 He of light source
Imaging device 21 deviates initial design position, and if serious, coaxial relation will receive destruction, and image quality is caused to reduce, and schemes
As that can not identify.It can the resulting structure problem by the scheme in following embodiment.
In a further embodiment, referring to Fig.1 3, the bottom surface of culturing room's upper cover 11 is provided with spring catch 112, spring catch
112 include guide 114, pin 113 and pressure spring 115, and guide 114 is cylinder, inside opens up step circular hole 116, step circular hole 116
Including the great circle hole section and roundlet hole section from top to bottom set gradually；The lower end of pin 113 is bulb, and small sircle hole is slidably arranged in
In section, the upper end of pin 113 is provided with the rotary table rank that diameter is greater than roundlet hole section, and rotary table rank is slidably arranged in great circle hole section；
Pressure spring 115 is arranged in great circle hole section, and lower end presses against on rotary table rank；Make pin 113 that there is certain initial pretightening force.
The taper hole 103 of the bulb cooperation insertion for supply and marketing 113, taper hole 103 and pin 113 are offered on culturing room's platform 10
Coaxial line.Spring catch 112 and taper hole 103 wear the influence for gap occur to eliminate hinge 17.
Spring catch 112 and taper hole 103 are respectively embedded in culturing room's upper cover 11 and culturing room's platform 10, and the two is coaxial
Line, the bulb of pin 113 and taper hole 103 play the role of guiding and positioning when closing lid, can limit culturing room's upper cover 11 and platform exists
Position at the point in horizontal plane is deviateed, and the influence in gap is eliminated.Pressure spring 115 can guarantee even if 17 gap of hinge increase the case where
Lower pin 113 can be engaged always with 103 column of taper hole.
The positioning of 103 column of spring catch 112 and taper hole can not limit the rotation offset of 11 opposed platforms of culturing room's upper cover, still
In conjunction with the located lateral function of locating piece 86 and square hole 472, the positional relationship of culturing room's upper cover 11 and platform can be uniquely determined,
Thoroughly eliminating the gap that hinge 17 is worn influences, and guarantees the reliability of image quality.
Embryo's time difference of the invention is imaged culture module and is primarily useful in embryo's time difference culture apparatus, incubates for embryo
It educates, light source and micro imaging system is generally also equipped in embryo's time difference culture apparatus, during shooting and record Embryo Culture
Time difference image.Referring to Fig.1 0, culture module application is imaged in embryo's time difference of the invention and cultivates dress in specific embryo's time difference
Set one of 9 signals.The embryo distribution of culture dish 2 at least two independent culture ponds 5, culture pond 5 by arranged in a straight line,
System structure is compact, and freedom of motion is few, by micro imaging system 91 be responsible for shooting and record Embryo Culture during when
Difference image, wherein light source 90 is located at 2 upside of culture dish, can independently move along X-axis, and micro-imaging equipment is located at 2 downside of culture dish,
It can independently move along X-axis and independently be moved with Z axis, the two is respectively moved along X-axis and positioned to sample to be tested region, is set by imaging
Standby Z axis mobile handoff imaging focal plane, then realizes the successively imaging to all samples.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on the figure or
Positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must
There must be specific orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (10)
1. culture module is imaged in a kind of embryo's time difference characterized by comprising culturing room's ontology and be set to culturing room's sheet
Intracorporal culture dish；
It include at least two culture ponds in the culture dish, the culture pond includes top pond body and lower part pond body, the top
The inner wall of pond body is connected with the inner wall of lower part pond body, and the inclination angle of the inner wall of the top pond body is less than the interior of the lower part pond body
The inclination angle of wall, wherein the inclination angle refers to the inner wall of the top pond body or lower part pond body and the angle of horizontal direction.
2. embryo's time difference according to claim 1 be imaged culture module, which is characterized in that the culture dish include ware body and
Ware lid, the ware body include the rectangular portion that rounded portions and width are less than the diameter of the rounded portions, at the side of the rectangular portion
In the inside of the rounded portions, the rounded portions are stretched out in the other side；
Cultivation region is provided on the ware body, the culture pond is arranged in the cultivation region；
The cultivation region by the bottom plate and the cultivation region coaming plate that is upwardly extended from the bottom plate of the ware body around being formed, the bottom
The recessed formation culture pond of plate, rinse bath and serial number mark.
3. culture module is imaged in embryo's time difference according to claim 2, which is characterized in that the bottom of the lower part pond body is set
It is equipped at least one sample micropore, the sample micropore includes upper hole and bottom outlet, and the internal diameter in the upper hole is greater than the bottom outlet
Internal diameter, and the inner wall of the bottom outlet and vertical direction have the not angle for 0 °.
4. culture module is imaged in embryo's time difference according to claim 2, which is characterized in that the ware lid is for being fastened on institute
It states on ware body comprising cover board and the ware lid coaming plate extended downwardly from cover board, it is described when the ware lid is fastened on the ware body
The cultivation region coaming plate of ware body is in the inside of the ware lid coaming plate.
5. culture module is imaged in embryo's time difference according to claim 4, which is characterized in that the ware lid further includes hand-held piece
And partition, the partition extend out from ware lid bottom surface, parallel with the cover board, the hand-held piece is vertically set on the partition
On；
Boss, when the ware lid is fastened on the ware body, the lid are provided at four vertex positions of the cover board lower wall
Plate is supported on the cultivation region coaming plate by four boss.
6. culture module is imaged in embryo's time difference according to claim 5, which is characterized in that be additionally provided with PH on the ware body
Measurement zone, trade mark area, label area and holding area.
7. culture module is imaged in embryo's time difference according to claim 1, which is characterized in that culturing room's ontology includes training
It supports room platform and culturing room's upper cover, the two constitutes closed sample culturing room；It offers on culturing room's platform for being arranged
The ware slot of the culture dish, the shape of the ware slot and the bottom shape of the culture dish match, and open among the ware slot
Equipped with the light tank for penetrating through culturing room's platform.
8. culture module is imaged in embryo's time difference according to claim 7, which is characterized in that the rear end of culturing room's upper cover
It is connect by rotary gemel with culturing room's platform, the bottom surface of culturing room's upper cover is provided with annular groove, in the annular groove
Filled with sealing strip, the front end of culturing room's ontology is additionally provided with open-and-close mechanism；
The open-and-close mechanism includes baffle, unlocking board, hinge axis, the hinge seat, the second drawing being arranged in the culturing room on lid
Spring, latch hook, locating piece and the optoelectronic switch being arranged on culturing room's platform, reed, reed briquetting；
The latch hook is integrally L-shaped, is from top to bottom disposed with hinge hole, tension spring hole and gib head, institute along its length thereon
The bottom for stating gib head end is equipped with bevelling, and the latch hook is cooperated by hinge hole and hinge axis, is rotatably provided in and is fixed in training
On the hinge seat for supporting room upper cover；
One end of second tension spring is connect with tension spring hole, and the other end is connect with the column being mounted in the culturing room on lid；
The locating piece is fixed on culturing room's upper cover bottom, is hollow structure, and the latch hook from top to bottom passes through the positioning
The gib head of block, the latch hook bottom exposes the locating piece.
9. culture module is imaged in embryo's time difference according to claim 8, which is characterized in that be on culturing room's platform
Rectangular opening is offered below the latch hook, is provided with bevel edge on side wall corresponding with the bevelling of the gib head on the rectangular opening,
The bevel edge bottom hollows out to form hook groove；
The reed is set to below the rectangular opening, L-shaped, and the short side of the reed is in below the gib head, described
Long side one end of reed is pushed down by reed briquetting, and the optoelectronic switch is set to the lower section of the short side of the reed；
It is additionally provided on the locating piece for cooperating the convex block being inserted into the rectangular opening, the two sides of the convex block are provided with
For the positioning straight flange with the cooperation of two inner vertical walls of the rectangular opening, after the convex block plugs the rectangular opening, the positioning
Straight flange is in the bevel edge two sides of the rectangular opening.
10. culture module is imaged in embryo's time difference according to claim 9, which is characterized in that the bottom of culturing room's upper cover
Face is provided with spring catch, and the spring catch includes guide, pin and pressure spring, and the guide is cylinder, inside opens up step circular hole,
Step circular hole includes the great circle hole section and roundlet hole section from top to bottom set gradually；The lower end of the pin is bulb, is slidably set
It sets in the roundlet hole section, the upper end of the pin is provided with the rotary table rank that diameter is greater than the roundlet hole section, the rotary table rank
Slidably it is arranged in the great circle hole section；The pressure spring is arranged in the great circle hole section, and lower end presses against the rotary table
On rank；
The taper hole for the bulb cooperation insertion for the pin is offered on culturing room's platform, the taper hole is coaxial with pin
Line.
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