CN103421682B - Automatic liquid changing culture dish for embryo - Google Patents
Automatic liquid changing culture dish for embryo Download PDFInfo
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- CN103421682B CN103421682B CN201210164790.8A CN201210164790A CN103421682B CN 103421682 B CN103421682 B CN 103421682B CN 201210164790 A CN201210164790 A CN 201210164790A CN 103421682 B CN103421682 B CN 103421682B
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- embryo
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- embryo culture
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Abstract
The invention discloses an automatic liquid changing culture dish for embryos. The culture dish comprises a dish body and a cover for enclosing an opening of the dish body. The culture dish is characterized in that an inner side wall of the culture dish body is suspended with an embryo culture chamber; the embryo culture is divided into an embryo culture zone and a waste liquid discharge zone; an overflow channel is arranged between the embryo culture zone and the waste liquid discharge zone; the bottom of the overflow channel is higher than the bottoms of the embryo culture zone and the waste liquid discharge zone; the dish cover is provided with a through hole for dropping fresh nutrient solution from outside the dish into the embryo culture zone; and when the liquid level of the nutrient solution in the embryo culture zone is higher than the bottom of the overflow channel, the nutrient solution in the embryo culture zone automatically flows into the waste liquid waste liquid discharge zone through the overflow channel. The culture dish provided by the invention can cooperate with an automatic liquid dropping pump to automatically and quantificationally provide nutrient solution and at the same time replace the waste liquid, provide required nutrients during an embryonic development process and reduce damages caused by repeated moving and liquid changing.
Description
Technical field
The present invention relates to a kind of medical instruments, relate in particular to a kind of embryo from moving liquid culture dish.
Background technology
Vitro culture is a key link in IVF (IVFET) technology, and the external environment of embryo growth directly affects growing of body early embryo, and then has influence on embryo's plantation success ratio.Embryo's vitro culture is a complicated process, and it is to the many factors in growing environment, as Air quality, temperature, nutrient solution composition and pH value and light etc. all have strict requirement.Whether tube baby's success or not is depended on to a great extent can have a stable culture systems, and when there being stable culture environment, the growth and development that embryo can be healthy, relatively improves embryo's availability.And in this stable system, embryo medium is being played the part of very important role, because it provides embryo to divide needed nutrient while growth.In embryo medium, be rich in for the needed each seed amino acid of fetal development, but embryo is its physiology and constantly variation of metabolism generation in culturing process, the ammonia that amino acid decomposes and embryo metabolism produces, to the toxic effect of embryo, therefore needs the successional embryo medium that provides also to replace waste liquid simultaneously.
Changing at present embryo medium does not have special method, and the simple exactly artificial imbibition device that passes through sucks nutrient solution.Each culture dish need to being taken out from incubator when changing nutrient solution just can be carried out afterwards, and such liquid process of changing has been destroyed stable culture systems virtually, and the embryo in culturing process has been caused to certain injury.
Summary of the invention
The object of the invention is to overcome the defect of prior art, provide a kind of embryo from moving liquid culture dish,
Comprise the ware lid of ware body and sealing ware body open-mouth, it is characterized in that: the outstanding embryo culture chamber of being equipped with of described ware body inner side-wall, indoor embryo culture district and the discharging of waste liquid district of being separated into of this embryo culture, between described embryo culture district and discharging of waste liquid district, be provided with overflow ducts, the bottom of this overflow ducts is higher than the bottom in described embryo culture district and discharging of waste liquid district;
Described ware lid is provided with for the external fresh medium of ware and supplements the through hole that splashes into described embryo culture district, when the liquid level of nutrient solution in described embryo culture district is during higher than described overflow ducts bottom, the nutrient solution in described embryo culture district flows into described discharging of waste liquid district through overflow ducts automatically.
The sidewall in described discharging of waste liquid district is provided with and prevents the waste liquid flow pass of waste reflux to described embryo culture district, and the bottom of this waste liquid flow pass is lower than the bottom of described overflow ducts.
The baffle for separating of described embryo culture chamber in the middle of being arranged on becomes described embryo culture district and discharging of waste liquid district, and the height of described dividing plate is lower than the height of other sidewall of described embryo culture chamber, but higher than the height of the short dividing plate of described discharging of waste liquid district one side; The above space of described dividing plate apical margin forms described overflow ducts, and the above space of described short dividing plate apical margin forms the waste liquid flow pass in described discharging of waste liquid district.
The external liquid changing device liquid output syringe needle activity of the through hole of described ware lid and ware pegs graft to supplement at regular time and quantity fresh medium to described embryo culture district.
The outstanding plural embryo culture chamber of being equipped with of described ware body inner side-wall.
On described ware body inner side-wall, be provided with T shape draw-in groove, the outdoor sidewall of described embryo culture convexes with the T shape card key that can peg graft with described T shape draw-in groove activity.
Described embryo culture chamber is taking described dividing plate as a sidewall, and it is highly 3/4ths of other face sidewall height, and described discharging of waste liquid district is taking described short dividing plate as a sidewall, and its height is lower than the height of described dividing plate.
Described ware lid inner-wall surface is to downward-extension one right cylinder, and described through hole runs through this right cylinder center; Described right cylinder both sides are extended with the lug for stationary cylinder.
Described embryo culture chamber is rectangular shape.
Compared with prior art, beneficial effect of the present invention is: needed nutrient in embryo development procedure is provided, reduce embryo in culturing process because the injury that mobile replacing nutrient solution repeatedly causes, the physiology machine that embryo can be met in body turns, improve the rate of forming of blastaea, also increased clinical pregnancy rate simultaneously.
Brief description of the drawings
With reference to accompanying drawing, the present invention will be better understood.Accompanying drawing is as follows:
Fig. 1 be embryo of the present invention from move liquid culture dish vertical view;
Fig. 2 be embryo of the present invention from move liquid culture dish along its A-A line sectional view;
Fig. 3 is that embryo of the present invention is from moving the vertical view of embryo culture chamber in liquid culture dish;
Fig. 4 be embryo of the present invention from move liquid culture dish ware body along its B-B line sectional view;
Fig. 5 is that embryo of the present invention is from the vertical view of moving the another kind of embodiment of liquid culture dish.
Embodiment
In order to make object of the present invention, technical scheme, beneficial effect clearer, below in conjunction with drawings and Examples, the present invention is described in further details.Should be appreciated that specific embodiment described herein, only for explaining the present invention, is not intended to limit the present invention.
Below in conjunction with specific embodiment, realization of the present invention is described in detail:
As depicted in figs. 1 and 2, a kind of embryo, from moving liquid culture dish, comprises ware lid 2 and the embryo culture chamber 11 of ware body 1, sealing ware body open-mouth.Embryo culture chamber 11 is divided into embryo culture district 112 and discharging of waste liquid district 113.Embryo culture chamber 11 is rectangular shape, is appreciated that embryo culture chamber also can adopt other shapes, as connected right cylinder etc.Between embryo culture district 112 and discharging of waste liquid district 113, be provided with overflow ducts 115, the bottom of this overflow ducts 115 is higher than the bottom in described embryo culture district 112 and discharging of waste liquid district 113.Overflow ducts 115 can be a circle or the passage of other shapes, can be also to form overflow ducts by the above space of dividing plate apical margin, is not limited to these embodiments, and the structure that can reach same effect all can.
Concrete, the dividing plate 111 in the middle of this embryo culture chamber 11 is arranged on is divided into embryo culture district 112 and discharging of waste liquid district 113, and discharging of waste liquid district 113 1 sides are provided with short dividing plate 114.The height of dividing plate 111 is lower than the height of embryo culture chamber 11 other sidewalls, but higher than the height of the short dividing plate 114 of described discharging of waste liquid district 113 1 sides.Embryo culture district 112 is taking 111 as sidewalls of described dividing plate, and preferred, it is highly 3/4ths of other face sidewall height, and discharging of waste liquid district 113 is taking 114 as sidewalls of described short dividing plate, and its height is lower than the height of described dividing plate 111.The dividing plate 111 above spaces of apical margin form overflow ducts 115, and the bottom of this overflow ducts 115 is higher than the bottom in embryo culture district 112 and discharging of waste liquid district 113.Short dividing plate 114 formation discharging of waste liquid districts 113, the above spaces of apical margin can prevent the waste liquid flow pass 116 of waste reflux to described embryo culture district 112, and the bottom of this waste liquid flow pass 116 is lower than the bottom of described overflow ducts 115.Described waste liquid flow pass 116 also can adopt other structures with identical function, as adopted the effigurate passage of cross section tool etc.
Embryo culture chamber 11 is outstanding to be contained on the inner side-wall of ware body 1.As shown in Figure 3 and Figure 4, on ware body 1 inner side-wall, be provided with vertical T shape draw-in groove 12, on 11 outer side walls of embryo culture chamber, convex with the T shape card key 117 that can peg graft with described T shape draw-in groove 12 activities.Be appreciated that embryo culture chamber 11 also can adopt other can conveniently fill the structure of getting and be plugged on ware body 1 sidewall, the mode activity that adopts dovetail-indent to match with dovetail card key as embryo culture chamber 11 is plugged on ware body 1.
Ware lid 2 inner-wall surfaces are to downward-extension one right cylinder 22, and right cylinder 22 both sides are extended with the lug 23 for stationary cylinder.Ware lid 2 is provided with for the external fresh medium of ware and supplements the through hole 21 that splashes into embryo culture district 112, and through hole 21 runs through the central position of right cylinder 22.External quantitative automatic drip pump output syringe needle (attached not shown) activity of the through hole 21 of ware lid 2 and ware pegs graft to supplement fresh medium to embryo culture district 112 at regular time and quantity.When the liquid level of embryo culture district 112 interior nutrient solutions is during higher than overflow ducts 115 bottom, the nutrient solution in described embryo culture district 112 flows into discharging of waste liquid district 113 through overflow ducts 115 automatically.
To change the process of enchylema as follows from moving liquid culture dish to utilize embryo:
The embryo that needs are cultivated is placed in the embryo culture district 112 that fills nutrient solution, and now, the liquid level of embryo culture district 112 interior nutrient solutions is lower than the height of dividing plate 111.Embryo is covered to ware lid 2 from moving liquid culture dish, and the output syringe needle (attached not shown) of the quantitative automatic drip pump one end that is connected with nutrient solution is inserted in the through hole 21 on ware lid 2, make to export the syringe needle interior supplementary fresh nutrient solution to embryo culture district 112 at regular time and quantity by the automatic drip pump of adjustment quantitative.
By constantly splashing into fresh nutrient solution, the nutrient solution in embryo culture district 112 constantly increases.When accumulating to a certain amount of time, the nutrient solution in embryo culture district 112 automatically overflows across dividing plate 111 and flow in the discharging of waste liquid district 113 being adjacent.
Under normal circumstances, discharging of waste liquid district 113 waste liquid that discharge in splendid attire embryo culture district 112 completely.But under abnormal conditions, as situations such as quantitative automatic drip pump break down, waste liquid can overflow across waste liquid flow pass 116 and flow in ware body 1, avoid waste reflux to embryo culture district 112 interior infringement embryo.
Fig. 5 is that embryo of the present invention is from the another kind of embodiment of moving liquid culture dish, this embryo is basic identical from moving liquid culture dish structure from moving the embryo shown in liquid culture dish and Fig. 1 to Fig. 4, difference is, ware body 1 inner side-wall is outstanding is equipped with two embryo culture chambers 11.In figure, just schematically show two embryo culture chambers 11, in fact can hang and fill plural embryo culture chamber 11.Such benefit is, multiple embryos of a couple who cultivates can be placed on to an embryo and cultivate from moving in liquid culture dish, avoids confusion.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. an embryo is from moving liquid culture dish, comprise the ware lid (2) of ware body (1) and sealing ware body open-mouth, it is characterized in that: the outstanding embryo culture chamber (11) of being equipped with of described ware body (1) inner side-wall, in this embryo culture chamber (11), be separated into embryo culture district (112) and discharging of waste liquid district (113), between described embryo culture district (112) and discharging of waste liquid district (113), be provided with overflow ducts (115), the bottom of this overflow ducts (115) is higher than the bottom of described embryo culture district (112) and discharging of waste liquid district (113);
Described ware lid (2) is provided with for the external fresh medium of ware and supplements the through hole (21) that splashes into described embryo culture district (112), when the liquid level of nutrient solution in described embryo culture district (112) is during higher than described overflow ducts (115) bottom, the nutrient solution in described embryo culture district (112) flows into described discharging of waste liquid district (113) through overflow ducts (115) automatically;
Dividing plate (111) in the middle of described embryo culture chamber (11) is arranged on is separated into described embryo culture district (112) and discharging of waste liquid district (113), the height of described dividing plate (111) is lower than the height of other sidewall of described embryo culture chamber (11), but higher than the height of the short dividing plate (114) of described discharging of waste liquid district (113) one sides; The above space of described dividing plate (111) apical margin forms described overflow ducts (115).
2. embryo as claimed in claim 1 is from moving liquid culture dish, it is characterized in that: the sidewall in described discharging of waste liquid district (113) is provided with and prevents the waste liquid flow pass (116) of waste reflux to described embryo culture district (112), and the bottom of this waste liquid flow pass (116) is lower than the bottom of described overflow ducts (115).
3. embryo as claimed in claim 2, from moving liquid culture dish, is characterized in that: the above space of described short dividing plate (114) apical margin forms the waste liquid flow pass (116) in described discharging of waste liquid district (113).
4. embryo as claimed in claim 3, from moving liquid culture dish, is characterized in that: the external liquid changing device liquid output syringe needle activity of the through hole (21) of described ware lid (2) and ware pegs graft to supplement at regular time and quantity fresh medium to described embryo culture district (112).
5. the embryo as described in claim 1 to 4 any one claim, from moving liquid culture dish, is characterized in that: the outstanding plural embryo culture chamber (11) of being equipped with of described ware body (1) inner side-wall.
6. the embryo as described in claim 1 to 4 any one claim is from moving liquid culture dish, it is characterized in that: on described ware body (1) inner side-wall, be provided with T shape draw-in groove (12), described embryo culture chamber (11) outer side wall convexes with the T shape card key (117) that can peg graft with described T shape draw-in groove (12) activity.
7. embryo as claimed in claim 3 is from moving liquid culture dish, it is characterized in that: described embryo culture district (112) is taking described dividing plate (111) as a sidewall, it is highly 3/4ths of other face sidewall height, described discharging of waste liquid district (113) is taking described short dividing plate (114) as a sidewall, and its height is lower than the height of described dividing plate (111).
8. the embryo as described in claim 1 to 4 any one claim, from moving liquid culture dish, is characterized in that: described ware lid (2) inner-wall surface is to downward-extension one right cylinder (22), and described through hole (21) runs through this right cylinder (22) central position; Described right cylinder (22) both sides are extended with the lug (23) for stationary cylinder (22).
9. the embryo as described in claim 1 to 4 any one claim, from moving liquid culture dish, is characterized in that: described embryo culture chamber (11) is rectangular shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210164790.8A CN103421682B (en) | 2012-05-25 | 2012-05-25 | Automatic liquid changing culture dish for embryo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210164790.8A CN103421682B (en) | 2012-05-25 | 2012-05-25 | Automatic liquid changing culture dish for embryo |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103421682A CN103421682A (en) | 2013-12-04 |
| CN103421682B true CN103421682B (en) | 2014-12-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210164790.8A Expired - Fee Related CN103421682B (en) | 2012-05-25 | 2012-05-25 | Automatic liquid changing culture dish for embryo |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109642200B (en) * | 2016-08-18 | 2023-04-04 | 普兰德有限两合公司 | Cell culture insert and device for culturing cells |
| CN110408540A (en) * | 2019-08-07 | 2019-11-05 | 中国科学院苏州生物医学工程技术研究所 | Embryo's changing liquid automatically culture dish |
| CN112662553B (en) * | 2020-12-29 | 2024-03-22 | 中国科学院苏州生物医学工程技术研究所 | Embryo culture device |
| CN112625907A (en) * | 2020-12-29 | 2021-04-09 | 中国科学院苏州生物医学工程技术研究所 | Embryo long-term culture system |
| CN112708554A (en) * | 2020-12-30 | 2021-04-27 | 刘仕兰 | Multi-functional intelligent cell culture dish |
| CN117660183B (en) * | 2024-01-31 | 2024-04-16 | 山东大学 | Embryo microfluidic dynamic culture dish and culture method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3660243A (en) * | 1970-03-16 | 1972-05-02 | North American Rockwell | Petri dish with compartment for sterilized spreading element |
| US3986935A (en) * | 1974-03-18 | 1976-10-19 | Miles Laboratories, Inc. | Biological chamber apparatus |
| CN2116698U (en) * | 1992-03-14 | 1992-09-23 | 谢明刚 | Dryness proof multi-function bacterium petri type dish |
| US5958762A (en) * | 1995-06-15 | 1999-09-28 | Chemodyne S.A. | Cell culture device |
| CN202744549U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Automatic solution replacing embryo culture dish |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007036611B4 (en) * | 2007-08-02 | 2015-10-08 | Deutsche Diabetes-Forschungsgesellschaft E.V. | Method and device for cultivating living cells |
-
2012
- 2012-05-25 CN CN201210164790.8A patent/CN103421682B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3660243A (en) * | 1970-03-16 | 1972-05-02 | North American Rockwell | Petri dish with compartment for sterilized spreading element |
| US3986935A (en) * | 1974-03-18 | 1976-10-19 | Miles Laboratories, Inc. | Biological chamber apparatus |
| CN2116698U (en) * | 1992-03-14 | 1992-09-23 | 谢明刚 | Dryness proof multi-function bacterium petri type dish |
| US5958762A (en) * | 1995-06-15 | 1999-09-28 | Chemodyne S.A. | Cell culture device |
| CN202744549U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Automatic solution replacing embryo culture dish |
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| CN103421682A (en) | 2013-12-04 |
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Owner name: ZHOU CANQUAN CAI JING CHEN WEIBIN Effective date: 20140515 |
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Effective date of registration: 20140515 Address after: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Applicant after: Zeng Yong Applicant after: Zhou Canquan Applicant after: Cai Jing Applicant after: Chen Weibin Address before: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Applicant before: Zeng Yong |
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