CN103505299B - Oral cavity in-site dental plaque collection device - Google Patents
Oral cavity in-site dental plaque collection device Download PDFInfo
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- CN103505299B CN103505299B CN201310445081.1A CN201310445081A CN103505299B CN 103505299 B CN103505299 B CN 103505299B CN 201310445081 A CN201310445081 A CN 201310445081A CN 103505299 B CN103505299 B CN 103505299B
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- oral cavity
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- dental plaque
- plaque
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Abstract
A kind of oral cavity in-site dental plaque collection device, comprise bacterial plaque disposable box and base, described bacterial plaque disposable box top is the cavity placing sample collection material, bottom is provided with the embeded slot with base combination, the top of described base is the chimera mated with the embeded slot in bacterial plaque disposable box, bottom is pedestal, is connected between pedestal with chimera by cylinder; During using state, the chimera on base inserts in the embeded slot of disposable box setting, and the cylinder around base is provided with retention means.This oral cavity in-site dental plaque collection device can obtain the complete dental plaque of three-D space structure, and the original position realizing dental plaque is collected.
Description
Technical field
The invention belongs to Dental Plaque collection technique field, the device of particularly a kind of oral cavity original position bacterial plaque collection.
Background technology
There is natural bacteria in oral cavity, of a great variety, known at present have more than 50 kinds different generas at least, and it adheres to body surface formation and has aggressive, drug-fast bacterial community, is dental plaque.Dental plaque can be considered the microecological environment of antibacterial, and antibacterial grows in this environment, grows, breeds and becomes feeble and die and carries out complicated metabolic activity wherein.Dental plaque can cause multiple oral disease, and as gingivitis, periodontitis, Chalk speckle, dental caries and halitosis etc., severe patient also can cause bacterial endocarditis.The correlational study of dental plaque becomes the most important thing of oral disease research undoubtedly, and the acquisition of dental plaque is the primary problem solved in research work.
The method of dental plaque is obtained for adopting sterilized instrument scraping dental plaque sample in existing dental plaque research work, though dental plaque main component can be obtained like this, but inevitably destroy the original three-D space structure of dental plaque, have a strong impact on carrying out and experiment effect of experimentation.
Summary of the invention
The object of the invention is to the Shortcomings overcoming prior art, provide a kind of oral cavity in-site dental plaque collection device, to obtain the complete dental plaque of three-D space structure, the original position realizing dental plaque is collected.
Oral cavity in-site dental plaque collection device of the present invention, comprise bacterial plaque disposable box and base, described bacterial plaque disposable box top is the cavity placing sample collection material, bottom is provided with the embeded slot with base combination, the top of described base is the chimera mated with the embeded slot in bacterial plaque disposable box, bottom is pedestal, is connected between pedestal with chimera by cylinder;
During using state, the chimera on base inserts in the embeded slot of bacterial plaque disposable box setting, and the cylinder around base is provided with retention means.
Above-mentioned oral cavity in-site dental plaque collection device, the cavity of described placement sample collection material is the untight cavity in top and a side.
Above-mentioned oral cavity in-site dental plaque collection device, what described bacterial plaque disposable box was arranged is dovetail groove with the embeded slot of base combination, and described dovetail groove is blind-hole type dovetail groove, and the shape and size of the chimeric shape and size that base is arranged and described dovetail groove match.
Above-mentioned oral cavity in-site dental plaque collection device, the pedestal lower surface of described base is to recessed cambered surface, and the radian of described cambered surface and the radian of facies buccalis dentis match.
Above-mentioned oral cavity in-site dental plaque collection device, described retention means is that mouth cavity orthodontic rubber divides tooth circle or mouth cavity orthodontic rustless steel ligature.
Above-mentioned oral cavity in-site dental plaque collection device, its length L, width B, respective heights h are 4mm≤L≤10mm, 2mm≤B≤5mm, height 2mm≤h≤5mm.
Above-mentioned oral cavity in-site dental plaque collection device, described base, the bacterial plaque disposable box material with biocompatibility makes, and preferably makes of rustless steel.The preferred global formation of manufacture method, makes the chimera of composition base, pedestal, cylinder be integrated, each several part one-shot forming of bacterial plaque disposable box.
Above-mentioned oral cavity in-site dental plaque collection device, the edge of described base, bacterial plaque disposable box need through processed, make its smooth circle blunt, to avoid causing damage to oral cavity.
Oral cavity in-site dental plaque collection device of the present invention using method in clinical experiment is as follows:
By sample collection material needed for experimentation (as hydroxy-apatite flag, enamel sheet etc.) be adhered in the cavity of placement sample collection material of bacterial plaque disposable box with the bonding agent not containing antibacterial bacteriostatic composition, then the chimera on base is inserted in the embeded slot arranged bottom bacterial plaque disposable box, with the cylinder of retention means around base, base and bacterial plaque collecting cassette are fixed together, continue after by conventional for animal or human's body oral cavity every wet, with the phosphoric acid acid etching facing to be sampled that the volumetric concentration that oral cavity uses is 37%, with orthodontic bracket bonding agent by bonding with the buccal surface of tooth for the cambered surface of bacterial plaque catcher base (as shown in Figure 9), namely the installation of bacterial plaque catcher of the present invention and sample collection material is completed.
Design according to different experiments, to again collect bacterial plaque, first in oral cavity, retention means is taken off, again the bacterial plaque disposable box obtaining bacterial plaque to be measured is taken off, then will be bonded with new bacterial plaque disposable box and the base combination of sample collection material as stated above and fix, the bacterial plaque disposable box namely only need changing bacterial plaque catcher can carry out original position bacterial plaque collection next time.
After having tested, oral cavity in-site dental plaque collection device entirety taken off from facing, if facing residual fraction bonding agent, bonding agent removed by available rubber cup etc., polishing facing.
Compared with prior art, the present invention has following beneficial effect:
1, because oral cavity in-site dental plaque collection device of the present invention can be fixed on the tooth of human or animal, make oral plaque self-assembling formation and be grown on the sample collection material in bacterial plaque disposable box, the original position realizing bacterial plaque is collected, thus the dental plaque of the complete and self-sow form of three-D space structure can be obtained, for the research of the composition of Dental Plaque, three-D space structure and follow-up intervention prophylactico-therapeutic measures etc. provides original reliable object of study.
2, because the base of oral cavity in-site dental plaque collection device of the present invention and bacterial plaque disposable box are for removably connecting, the bacterial plaque disposable box having obtained bacterial plaque to be measured only need be taken off when experimentation repeatedly need collect bacterial plaque, bacterial plaque next time can be carried out collect fixing with intraoral base after new bacterial plaque disposable box bonding sample collection material, thus not only reduce installation procedure, and can use cost be reduced because of reusing of base.
3, oral cavity in-site dental plaque collection device volume of the present invention is little, and the smooth of the edge circle is blunt, and has biocompatibility, thus can not cause any interference to the orthobiosis of animal or human.
4, oral cavity in-site dental plaque collection device structure of the present invention is simple, is convenient to processing and fabricating.
Accompanying drawing explanation
Fig. 1 is the front view of the bacterial plaque disposable box of oral cavity in-site dental plaque collection device of the present invention;
Fig. 2 is the top view of Fig. 1;
Fig. 3 is the left view of Fig. 1;
Fig. 4 is the front view of the base of oral cavity in-site dental plaque collection device of the present invention;
Fig. 5 is left view or the right view of Fig. 4;
Fig. 6 is the front view of oral cavity in-site dental plaque collection device of the present invention;
Fig. 7 is the left view of Fig. 6;
Fig. 8 is the right view of Fig. 6;
Fig. 9 is the using state figure of oral cavity in-site dental plaque collection device of the present invention.
In figure, cavity, 12-embeded slot, 2-base, 21-pedestal, 22-cylinder, 23-chimera, 3-retention means, 4-tooth, the 5-oral cavity in-site dental plaque collection device of 1-bacterial plaque disposable box, 11-placement sample collection material.
Detailed description of the invention
Below by embodiment, oral cavity in-site dental plaque collection device of the present invention is described further.
Embodiment 1
In the present embodiment, the structure of described oral cavity in-site dental plaque collection device, as shown in Fig. 1 ~ 8, comprises bacterial plaque disposable box 1 and base 2.Described bacterial plaque disposable box 1 top is the cavity 11 of the untight placement sample collection in top and side material, and bottom is provided with the embeded slot 12 with base combination, and described embeded slot is blind-hole type dovetail groove.The top of described base 2 is the chimera 23 matched with the dove tail shape in bacterial plaque disposable box and size, bottom is pedestal 21, pedestal lower surface is to recessed cambered surface, and the radian of this cambered surface and the radian of facies buccalis dentis match, and are connected between pedestal 21 with chimera 23 by cylinder 22.The material of bacterial plaque disposable box 1 and base 2 is rustless steel, respectively by casting global formation.
During using state, chimera 23 on base 2 inserts in the embeded slot 12 of bacterial plaque disposable box 1 setting, cylinder 22 around base is provided with retention means 3, described retention means is that mouth cavity orthodontic rubber divides tooth circle, the length L of the oral cavity in-site dental plaque collection device combined is 10mm, width B is 5mm, and height h is 5mm.
Embodiment 2
In the present embodiment, the difference of described oral cavity in-site dental plaque collection device and embodiment 1 is: described retention means 3 is the rustless steel ligature of mouth cavity orthodontic 0.22mm.The length L of the oral cavity in-site dental plaque collection device combined is 4mm, and width B is 2mm, and height h is 2mm.
Claims (7)
1. an oral cavity in-site dental plaque collection device, it is characterized in that comprising bacterial plaque disposable box (1) and base (2), described bacterial plaque disposable box (1) top is the cavity (11) placing sample collection material, bottom is provided with and the embeded slot of base combination (12), the top of described base (2) is the chimera (23) mated with the embeded slot in bacterial plaque disposable box, bottom is pedestal (21), connected by cylinder (22) between pedestal (21) with chimera (23), the pedestal lower surface of base (2) is to recessed cambered surface, the radian of described cambered surface and the radian of facies buccalis dentis match,
During using state, in the embeded slot (12) that chimera insertion bacterial plaque disposable box (1) on base (2) is arranged, cylinder (22) around base is provided with retention means (3), and base is bonded in human or animal's tooth buccal surface by pedestal lower surface is collected to realize oral cavity original position bacterial plaque.
2. oral cavity in-site dental plaque collection device according to claim 1, is characterized in that the cavity (11) placing sample collection material is the untight cavity in top and a side.
3. oral cavity in-site dental plaque collection device according to claim 1 or 2, what it is characterized in that bacterial plaque disposable box (1) arranges is dovetail groove with the embeded slot (12) of base combination, described dovetail groove is blind-hole type dovetail groove, and the shape and size of chimera (23) that base (2) is arranged and the shape and size of described dovetail groove match.
4. oral cavity in-site dental plaque collection device according to claim 1 or 2, is characterized in that retention means (3) divides tooth circle or mouth cavity orthodontic rustless steel ligature for mouth cavity orthodontic rubber.
5. oral cavity in-site dental plaque collection device according to claim 3, is characterized in that retention means (3) divides tooth circle or mouth cavity orthodontic rustless steel ligature for mouth cavity orthodontic rubber.
6. oral cavity in-site dental plaque collection device according to claim 1 or 2, is characterized in that the chimera (23) of base, pedestal (21) and cylinder (22) are integrated.
7. oral cavity in-site dental plaque collection device according to claim 1 or 2, is characterized in that its length L, width B, respective heights h are 4mm≤L≤10mm, 2mm≤B≤5mm, height 2mm≤h≤5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310445081.1A CN103505299B (en) | 2013-09-26 | 2013-09-26 | Oral cavity in-site dental plaque collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310445081.1A CN103505299B (en) | 2013-09-26 | 2013-09-26 | Oral cavity in-site dental plaque collection device |
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| Publication Number | Publication Date |
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| CN103505299A CN103505299A (en) | 2014-01-15 |
| CN103505299B true CN103505299B (en) | 2015-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310445081.1A Expired - Fee Related CN103505299B (en) | 2013-09-26 | 2013-09-26 | Oral cavity in-site dental plaque collection device |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105455856B (en) * | 2015-12-26 | 2018-01-26 | 浙江大学 | Plaque bio-film intraoral model in situ and preparation method thereof |
| CN109009235B (en) * | 2018-06-22 | 2023-10-13 | 四川大学 | Subgingival anaerobic flora collector and use method thereof |
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| EP0952211A1 (en) * | 1998-04-24 | 1999-10-27 | Millipore S.A. | Method for detecting micro-organisms and cartridge suitable for implementing it |
| CN101294135A (en) * | 2007-04-26 | 2008-10-29 | 米利波尔公司 | Microbiological analysis assembly and method |
| CN202744549U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Automatic solution replacing embryo culture dish |
| CN203048954U (en) * | 2013-02-05 | 2013-07-10 | 齐齐哈尔大学 | Biological cell culturing apparatus |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3892743B2 (en) * | 2002-03-01 | 2007-03-14 | 日本碍子株式会社 | Reaction cell and method of use thereof |
| ZA200602094B (en) * | 2006-01-16 | 2007-11-28 | Reliance Life Sciences Pvt Ltd | Device for culturing and transporting cells |
| CN202089986U (en) * | 2011-06-10 | 2011-12-28 | 朴美花 | Disposable fungus culture vessel |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0952211A1 (en) * | 1998-04-24 | 1999-10-27 | Millipore S.A. | Method for detecting micro-organisms and cartridge suitable for implementing it |
| CN101294135A (en) * | 2007-04-26 | 2008-10-29 | 米利波尔公司 | Microbiological analysis assembly and method |
| CN202744549U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Automatic solution replacing embryo culture dish |
| CN203048954U (en) * | 2013-02-05 | 2013-07-10 | 齐齐哈尔大学 | Biological cell culturing apparatus |
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| CN103505299A (en) | 2014-01-15 |
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