CN109009235B - Subgingival anaerobic flora collector and use method thereof - Google Patents
Subgingival anaerobic flora collector and use method thereof Download PDFInfo
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- CN109009235B CN109009235B CN201810652633.9A CN201810652633A CN109009235B CN 109009235 B CN109009235 B CN 109009235B CN 201810652633 A CN201810652633 A CN 201810652633A CN 109009235 B CN109009235 B CN 109009235B
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Abstract
The application discloses a subgingival anaerobic flora collector and a using method thereof. The subgingival anaerobic flora collector comprises a cylindrical shell of the collector, a large push rod with a thin needle tube-like structure, a small push rod with a collection wire, a sealing rubber ring, a collection wire, a plastic cover for sealing an outlet, protective gas, an inner ring spring, an outer ring spring and a top pin type telescopic mechanism; one end of the cylindrical shell of the collector is provided with a sealing rubber ring, and the other end of the cylindrical shell of the collector is provided with a plastic cover for sealing an outlet; the plastic cover of the sealing outlet is internally provided with a protection rubber film which is positioned at the bottom of the cylindrical shell of the collector and used for sealing the bottom of the cylindrical shell of the collector. The subgingival anaerobic flora collector provided by the application can ensure that subgingival anaerobic flora is completely protected by the protective gas in the collecting and transferring processes until inoculation, and is effectively combined with the depth range of detection obtained by initial examination, so that bleeding caused by excessive penetration of collecting wires is avoided, and flora collection is more accurate.
Description
Technical Field
The application relates to the technical field of oral medical appliances, in particular to a subgingival anaerobic flora collector and a method for completing collection by using the same.
Background
In the study of oral bacterial infection diseases such as periodontal disease, it is generally necessary to collect a bacterial population at a specific site, compare the bacterial population with a bacterial population at the same site in a normal person, and analyze the effect of changes in the number and composition properties of the bacterial population on the development of the disease. The frequent collecting part in clinical examination is periodontal pocket caused by periodontal disease, and the collecting method comprises paper tip method, standard ring method and inflatable sleeve method.
The paper tip method is to select paper tips of different types according to the width of periodontal pocket, insert the paper tips to the bottom of the pocket, take out the paper tips after 30 seconds, put the paper tips into a specimen bottle, transfer the specimen bottle to a laboratory for inoculation, and blow nitrogen locally in the bottle, and the paper tip method is mainly used for collecting non-attached subgingival bacterial groups. However, the paper tip is easily soaked and softened by gingival crevicular fluid, and the tip of the tooth Zhou Daigen with more periodontal pathogen group aggregation is difficult to reach.
The standard loop method is to make a collector similar to a bacterial inoculation loop with the loop diameter of 1mm by using orthodontic ligature wires, insert a small loop to the bottom of a periodontal pocket, collect bacterial flora and blow nitrogen locally. However, when the flora is collected in the molar area, the oral angle and the nitrogen blowing gas are required to be simultaneously pulled, so that the operation is inconvenient; and the manufacturing quality of the standard ring is relevant to doctors, and the diameters of the standard ring are required to be correspondingly adjusted aiming at gingival sulcus and periodontal pockets with different depths, so that the clinical time is occupied.
The inflatable sleeve method is to use an injection needle as a sleeve, wherein a metal wire needle core is arranged in the sleeve, barbs are formed at the tail ends of the wires and covered with calcium alginate, the sleeve is inserted into a periodontal pocket during sampling, and then the needle core is pushed out to collect bacterial groups in the pocket; and meanwhile, oxygen-free gas is introduced into the sleeve. However, the device is complicated to manufacture, and incorrect control of the distal needle core when extended can cause bleeding in the bag, increasing the risk of infection.
The existing collection method has the defect that the collection and transportation processes of anaerobic flora cannot be completely guaranteed under an anaerobic environment, and the anaerobic flora can be reduced in activity or even dead.
Disclosure of Invention
The application aims at: aiming at the problems, the subgingival anaerobic flora collector is provided, and can collect bacterial colonies conveniently and rapidly and completely ensure that the anaerobic flora is collected and transported in an anaerobic environment, which can lead to the reduction of activity and even death of certain anaerobic flora.
The technical scheme adopted by the application is as follows:
the subgingival anaerobic flora collector comprises a cylindrical collector shell (1), a large push rod (2) with a thin needle tube-like structure, a small push rod (3) with a collection wire, a sealing rubber ring (4), a collection wire (5), a sealing outlet cover (6), protective gas (7), an inner ring spring (8 a), an outer ring spring (8 b) and a top pin type telescopic mechanism (9);
one end of the cylindrical shell (1) of the collector is provided with a sealing rubber ring (4), and the other end of the cylindrical shell is provided with a sealing outlet cover (6); a protection rubber film is arranged in the sealing outlet cover (6), and the sealing outlet cover (6) and the rubber film thereof are positioned at the bottom of the cylindrical shell (1) of the collector and are used for sealing the bottom of the cylindrical shell (1) of the collector;
one end of the large push rod (2) with the thin needle tube-like structure is provided with a first push disc (2 c), the other end of the large push rod (2) with the thin needle tube-like structure is provided with a needle head (2 a), and the large push rod (2) with the thin needle tube-like structure is arranged in the cylindrical shell (1) of the collector in a sliding manner, so that the large push rod (2) with the thin needle tube-like structure can reciprocate in the cylindrical shell (1) of the collector under the help of external force;
the small push rod (3) with the collection wire is fixedly provided with the collection wire (5) at one end, the second push plate (3 b) is arranged at the other end, the small push rod (3) with the collection wire is arranged in the large push rod (2) with the thin needle tube sample structure in a sliding mode, and the small push rod (3) with the collection wire can reciprocate in the large push rod (2) with the thin needle tube sample structure by means of the small push rod (3) with the collection wire under external force.
The outer walls of one ends of the needle head (2 a) and the large push rod (2) with the thin needle tube-like structure are provided with zero graduation marks (2 b) and a plurality of uniformly distributed black graduation marks (3 a), wherein the positions of the zero graduation marks (2 b) are horizontal positions of the fixed connection positions of the large push rod (2) and the needle head (2 a), and the horizontal positions of the fixed connection positions of the small push rod (3) and the acquisition wire (5) are also horizontal positions of the fixed connection positions.
After the sealing outlet cover (6) is opened, the needle head (2 a) can be pushed to penetrate out of the rubber membrane by the large push rod (2) with the thin needle tube-like structure.
A closed space is formed between the inner wall of the cylindrical shell (1) of the collector and the outer wall of the large push rod (2) with the thin needle tube-like structure, and protective gas (7) is filled into the closed space to ensure that subgingival anaerobic flora is completely protected by the protective gas in the collecting and transferring processes until the inoculating process. Further, the filled shielding gas (7) is nitrogen.
The novel needle tube type automatic pushing device is characterized in that a top pin type telescopic mechanism (9) is arranged between the large push rod (2) with the thin needle tube type structure and the small push rod (3) with the collection wire, the top pin type telescopic mechanism (9) is located inside the cylindrical shell (1) of the collector and is arranged at one end, close to the sealing rubber ring (4), on the outer wall of the small push rod (3) with the collection wire, of the large push rod (2) with the thin needle tube type structure and the small push rod (3) with the collection wire are locked in an extending state after being pushed out simultaneously through the top pin type telescopic mechanism (9), and the novel needle tube type automatic pushing device returns to an initial position after being pressed again.
The inner ring spring (8 a) is positioned at the inner side of the large push rod (2) with the thin needle tube-like structure, the lower end of the inner ring spring is fixedly connected with the inner wall of the large push rod (2) with the thin needle tube-like structure, and the upper end of the inner ring spring is fixedly connected with the outer wall of the small push rod (3) with the collection wire;
the outer ring spring (8 b) is positioned at the inner side of the cylindrical shell (1) of the collector, the upper end of the outer ring spring is fixedly connected with the large push rod (2) with the thin needle tube-like structure at the position of a zero scale mark (2 b), and the lower end of the outer ring spring is fixedly connected with the bottom end of the inner wall of the cylindrical shell (1) of the collector.
After a second pushing disc (3 b) of the small push rod (3) with the collection wire is pressed down, the outer ring spring (8 b) is compressed until the front end needle head (2 a) completely penetrates out of the rubber membrane, and then the second pushing disc (3 b) is continuously pressed until the inner ring spring (8 a) is completely compressed, and the collection wire (5) completely extends out of the needle head (2 a); after the hands are loosened, the inner ring spring (8 a) and the outer ring spring (8 b) are respectively restored to the natural state, and the needle head (2 a) and the collection wire (5) are retracted to the initial positions.
The collecting wire (5) is provided with a villus opening structure, and the sealing outlet cover (6) is internally provided with sealing glue with the thickness of 1 mm.
A method of harvesting using a subgingival anaerobic flora harvester of any one of claims 1-9, the method comprising the steps of:
firstly, a sealing outlet cover (6) at the front section of the collector is taken down, the body of the collector is held by hands, the outlet of a collection wire (5) is aligned with a gingival sulcus or periodontal pocket, a large push rod (2) with a thin needle tube-like structure is pushed, meanwhile, a needle head (2 a) is pushed out from a rubber membrane, and zero scale marks (2 b) and black scale marks (3 a) on the outer wall of the large push rod (2) with the thin needle tube-like structure are slowly revealed in the process of pushing out the needle head (2 a) by the large push rod (2) with the thin needle tube-like structure;
the needle head (2 a) is positioned by observing the exposed scale values during collection, anaerobic flora of gingival sulcus or periodontal pocket is collected when the exposed scale values reach a certain value, and the positioned needle head (2 a) is locked by the ejector pin type telescopic mechanism (9);
the small push rod (3) with the collection wire is continuously pushed, the collection wire (5) at the front end of the thin rod-shaped structure is driven to continuously extend out of the needle head (2 a) and extend into the gingival sulcus or periodontal pocket, and the depth of the collection wire extending into the sulcus or pocket is determined;
the villus structure on the collection silk (5) adsorbs the flora gathered in the ditch or the bag, and after 30 seconds, the small push rod (3) with the collection silk is pressed down by force and then loosened, the small push rod (3) with the collection silk is retracted first, then the large push rod (2) with the fine needle tube sample structure is retracted, and the outlet cover (6) is covered to complete the collection operation of the subgingival anaerobic flora.
In summary, due to the adoption of the technical scheme, the beneficial effects of the application are as follows:
1. the subgingival anaerobic flora collector is a finished product collector for providing an anaerobic environment, and ensures that subgingival anaerobic flora is completely protected by nitrogen in the collecting and transferring processes until inoculation.
2. The collector can quantitatively control the depth of the collection wire extending into gingival sulcus or periodontal pocket, and effectively combines with the detection depth range obtained by initial examination, thereby avoiding bleeding caused by excessive extending of the collection wire.
3. The collector can easily extend into the gingival sulcus or the root tip of the periodontal pocket bacteria cluster, so that the bacteria collection is more accurate.
4. The collector can save the collected flora in a short time, and ensure the quality of the flora.
Drawings
FIG. 1 is a schematic side view of a device according to an embodiment of the present application;
fig. 2 is a schematic diagram of a front view structure of a device according to an embodiment of the present application;
reference numerals in the drawings are described as follows: 1-collector cylindrical shell, 2-big push rod with fine needle tube sample structure, 3-small push rod with collection wire, 4-sealing rubber ring, 5-collection wire, 6-sealing outlet cover, 7-protective gas, 8 a-inner ring spring, 8 b-outer ring spring, 9-ejector pin type telescopic mechanism, 2 a-needle, 2 b-zero scale mark, 2 c-first push disc, 3 a-black scale mark and 3 b-second push disc.
Detailed Description
In order to make the technical solution of the present application better understood by those skilled in the art, the technical solution of the present application will be clearly and completely described in the following with reference to the accompanying drawings, and based on the embodiments of the present application, other similar embodiments obtained by those skilled in the art without making any inventive effort should be included in the scope of protection of the present application.
Example 1
As shown in fig. 1-2, the subgingival anaerobic flora collector comprises a cylindrical shell 1 of the collector, a large push rod 2 with a fine needle tube-like structure, a small push rod 3 with a collection wire, a sealing rubber ring 4, a collection wire 5, a sealing outlet cover 6, and a protective gas 7, an inner ring spring 8a, an outer ring spring 8b and a top pin type telescopic mechanism 9 filled in the collector; the utility model discloses a high-speed harvester, including the cylindric shell 1 of collector, the cylindric shell 1 of collector one end is provided with sealed rubber band 4, and the other end is provided with the plastics sealed outlet cover 6 of taking the rubber membrane of sealed export, take big push rod 2 one end of thin needle tubing appearance structure to be provided with first push disc 2c, and the other end is equipped with syringe needle 2a, is equipped with zero scale mark 2b and evenly distributed's black scale mark 3a on the outer wall of syringe needle 2a and take big push rod 2 one end of thin needle tubing appearance structure, and the position of zero scale mark 2b is the horizontal position that big push rod 2 and syringe needle 2a fixed connection place are located, is the horizontal position that little push rod 3 and collection silk 5 fixed connection place are located simultaneously. The sealing outlet cover 6 is internally provided with a protection rubber film, and the sealing outlet cover 6 and the rubber film thereof are positioned at the bottom of the cylindrical shell 1 of the collector and used for sealing the bottom of the cylindrical shell 1 of the collector. After the sealing outlet cover 6 is opened, the large push rod 2 with a thin needle tube-like structure can push the needle head 2a to penetrate out of the rubber membrane; the large push rod 2 with the thin needle tube-like structure is arranged in the cylindrical shell 1 of the collector in a sliding way, so that the large push rod 2 with the thin needle tube-like structure can reciprocate in the cylindrical shell 1 of the collector under the help of external force; the small push rod 3 with the collection wire is fixedly provided with the collection wire 5 at one end and provided with the second push disc 3b at the other end, and the small push rod 3 with the collection wire is slidably arranged in the large push rod 2 with the thin needle tube sample structure and can reciprocate in the large push rod 2 with the thin needle tube sample structure by means of the small push rod 3 with the collection wire under external force. A closed space is formed between the inner wall of the cylindrical shell 1 of the collector and the outer wall of the large push rod 2 with the thin needle tube-like structure, and protective gas 7 is filled into the closed space to ensure that subgingival anaerobic flora is completely protected by the protective gas in the collecting and transferring processes until inoculation. In one embodiment the filling gas 7 is nitrogen.
The novel small push rod device is characterized in that a top pin type telescopic mechanism 9 is arranged between the large push rod 2 with the thin needle tube sample structure and the small push rod 3 with the collection wire, the top pin type telescopic mechanism 9 is located inside the cylindrical shell 1 of the collector and is arranged at one end, close to the sealing rubber ring 4, on the outer wall of the small push rod 3 with the collection wire, the large push rod 2 with the thin needle tube sample structure and the small push rod 3 with the collection wire are locked in an extending state after being pushed out simultaneously through the top pin type telescopic mechanism 9, and the novel small push rod device returns to the initial position after being pressed again.
The push pin type telescopic mechanism 9 is of an automatic locking structure similar to a ball pen, is arranged on the inner side of the large push rod 2 with the thin needle tube-like structure and the outer wall of the small push rod 3 with the collection wire and is close to the sealing rubber ring 4, the push pin type telescopic mechanism 9 consists of an indexing drum, a sliding claw sleeve and a spring, the front end of the sliding claw sleeve is provided with the sliding claw, the rear end of the indexing drum is provided with a plurality of indexing claws, deep grooves and shallow grooves are formed between the indexing claws, the two grooves are staggered, and the large push rod 2 with the thin needle tube-like structure and the small push rod 3 with the collection wire are locked in an extending state after being pushed out simultaneously through the push pin type telescopic mechanism 9 and are retracted after being pressed again.
The inner ring spring 8a is positioned at the inner side of the large push rod 2 with the thin needle tube-like structure, the lower end of the inner ring spring is fixedly connected with the inner wall of the large push rod 2 with the thin needle tube-like structure, and the upper end of the inner ring spring is fixedly connected with the outer wall of the small push rod 3 with the collection wire; the outer ring spring 8b is positioned on the inner side of the cylindrical shell 1 of the collector, the upper end of the outer ring spring is fixedly connected with the large push rod 2 with the thin needle tube-like structure at the position of the zero scale mark 2b, and the lower end of the outer ring spring is fixedly connected with the bottom end of the inner wall of the cylindrical shell 1 of the collector.
When the device is used, after the second pushing disc 3b of the small push rod 3 with the collection wire is pressed, the outer ring spring 8b is compressed until the front end needle head 2a completely penetrates out of the rubber membrane, and then the second pushing disc 3b is continuously pressed until the inner ring spring 8a is completely compressed, and the collection wire 5 completely extends out of the needle head 2 a; after the hands are loosened, the inner ring spring 8a and the outer ring spring 8b are respectively restored to the natural state, and the needle head 2a and the collection wire 5 are retracted to the initial positions.
In one embodiment, the overall length of the cylindrical shell 1 of the collector is 20mm, the outer diameter is 4mm, the inner diameter is 3mm, the front end is sealed by a sealing outlet cover 6 with the thickness of 1mm and a rubber film thereof, and the other end is fixedly sealed by a sealing rubber ring 4 with the outer diameter of 3mm, the inner diameter of 2.5mm and the thickness of 1 mm;
in one embodiment, the total length of the large push rod 2 with the thin needle tube sample structure is 25mm, the outer diameter is 2.5mm, the inner diameter is 1.5mm, the front needle head 2a is 5mm, the needle point can be exposed outwards at the zero graduation line 2b of the shell after the front needle head is completely extended, the diameter of the first push disc 2c is 5mm, the part of the large push rod 2 with the thin needle tube sample structure, which extends into the cylindrical shell 1 of the collector, and the part of the small push rod 3 with the collection wire, which extends into the large push rod 2 with the thin needle tube sample structure, are respectively and fixedly sealed by a sealing rubber ring 4 consisting of two parts of the outer diameter of 3mm, the inner diameter of 2.5mm, the thickness of 1mm, the outer diameter of 1.5mm and the inner diameter of 1 mm;
in one embodiment, the total length of the small push rod 3 with the collection wire is 30mm, the diameter is 1mm, the front collection wire is 5mm long, the thin rod is provided with black graduation marks 3a, the mark line 0 is flush with the zero graduation mark 2b on the large push rod 2 with the thin needle tube-like structure when the small push rod 3 with the collection wire is not moved from 0 to 5mm, and the diameter of the second push disc 3b at one end of the small push rod 3 with the collection wire is 2.5mm; the junction of the small push rod 3 with the collection wire and the large push rod 2 with the thin needle tube sample structure is provided with a push pin type telescopic mechanism 9.
In one embodiment, the acquisition wire 5 is 5mm long with a villiated open structure thereon.
In one embodiment, the sealing outlet cover 6 has a 1mm thick sealant therein, so that the cylindrical housing 1 of the collector can be tightly covered.
Example 2
The embodiment is used for specifically explaining a use method of the collector obtained based on any one of the foregoing embodiments:
firstly, a sealing outlet cover 6 at the front section of the collector is taken down, the collector body is held by hand, the outlet of a collection wire 5 is aligned to a healthy gingival sulcus or a periodontal pocket, a large push rod 2 with a thin needle tube-like structure is pushed, meanwhile, a needle head 2a is pushed out from a rubber membrane, in the process of pushing the needle head 2a out by the large push rod 2 with the thin needle tube-like structure, zero scale marks 2b and other black scale marks 3a on the outer wall of the large push rod 2 with the thin needle tube-like structure are gradually revealed, during conventional collection, the needle head 2a is required to be positioned by observing the revealed scale values, the collection of anaerobic bacteria groups of the gingival sulcus or the periodontal pocket can be achieved by the commonly revealed scale values of 2mm, and then the positioned needle head 2a with 2mm is locked by a push pin type telescopic mechanism 9; continuously pushing the small push rod 3 with the collection wire to drive the collection wire 5 at the front end of the thin rod-shaped structure to continuously extend out of the needle head 2a and extend into a gingival sulcus or periodontal pocket, and determining the depth extending into the sulcus or pocket according to the scale on the small push rod 3 with the collection wire; the villus structure on the collection wire 5 adsorbs the bacteria group gathered in the ditch or bag, especially root tip, after 30 seconds, the small push rod 3 with the collection wire is pressed down with force and then loosened, the small push rod 3 with the collection wire is retracted first, then the large push rod 2 with the fine needle tube-like structure is retracted, and the outlet cover 6 is covered and sealed, thus completing the collection operation of the subgingival anaerobic bacteria group.
The application is not limited to the specific embodiments described above. The application extends to any novel one, or any novel combination, of the features disclosed in this specification, as well as to any novel one, or any novel combination, of the steps of the method or process disclosed.
All of the features disclosed in this specification, or all of the steps in a method or process disclosed, may be combined in any combination, except for mutually exclusive features and/or steps.
This specification includes any feature disclosed in any accompanying claims and abstract which may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise. That is, each feature is one example only of a generic series of equivalent or similar features, unless expressly stated otherwise.
Claims (10)
1. The subgingival anaerobic flora collector is characterized by comprising a cylindrical collector shell (1), a large push rod (2) with a thin needle tube-like structure, a small push rod (3) with a collection wire, a sealing rubber ring (4), a collection wire (5), a sealing outlet cover (6), protective gas (7), an inner ring spring (8 a), an outer ring spring (8 b) and a top pin type telescopic mechanism (9); the inner ring spring (8 a) is positioned at the inner side of the large push rod (2) with the thin needle tube-like structure, and the outer ring spring (8 b) is positioned at the inner side of the cylindrical shell (1) of the collector;
one end of the cylindrical shell (1) of the collector is provided with a sealing rubber ring (4), and the other end of the cylindrical shell is provided with a sealing outlet cover (6); a protection rubber film is arranged in the sealing outlet cover (6), and the sealing outlet cover (6) and the rubber film thereof are positioned at the bottom of the cylindrical shell (1) of the collector and are used for sealing the bottom of the cylindrical shell (1) of the collector;
a closed space is formed between the inner wall of the cylindrical shell (1) of the collector and the outer wall of the large push rod (2) with the thin needle tube-like structure, and protective gas (7) is filled in the closed space; a top pin type telescopic mechanism (9) is arranged between the large push rod (2) with the thin needle tube sample structure and the small push rod (3) with the collection wire;
one end of the large push rod (2) with the thin needle tube-like structure is provided with a first push disc (2 c), the other end of the large push rod (2) with the thin needle tube-like structure is provided with a needle head (2 a), and the large push rod (2) with the thin needle tube-like structure is arranged in the cylindrical shell (1) of the collector in a sliding manner, so that the large push rod (2) with the thin needle tube-like structure can reciprocate in the cylindrical shell (1) of the collector under the help of external force;
the small push rod (3) with the collection wire is fixedly provided with the collection wire (5) at one end, the second push plate (3 b) is arranged at the other end, the small push rod (3) with the collection wire is arranged in the large push rod (2) with the thin needle tube sample structure in a sliding mode, and the small push rod (3) with the collection wire can reciprocate in the large push rod (2) with the thin needle tube sample structure by means of the small push rod (3) with the collection wire under external force.
2. The subgingival anaerobic flora collector of claim 1, wherein zero graduation marks (2 b) and a plurality of uniformly distributed black graduation marks (3 a) are arranged on the outer wall of one end of the needle head (2 a) and the large push rod (2) with the thin needle tube-like structure, and the position of the zero graduation marks (2 b) is the horizontal position of the fixed connection part of the large push rod (2) and the needle head (2 a) and the horizontal position of the fixed connection part of the small push rod (3) and the collecting wire (5).
3. A subgingival anaerobic flora harvester according to claim 1, in which the needle (2 a) is pushed out of the rubber membrane by a large push rod (2) with a thin needle-like structure after opening the sealing outlet cover (6).
4. A subgingival anaerobic flora harvester according to claim 1, wherein subgingival anaerobic flora is ensured to be completely protected by a protective gas during harvesting and transport until inoculation.
5. A subgingival anaerobic flora harvester according to claim 4, in which the filled shielding gas (7) is nitrogen.
6. A subgingival anaerobic flora harvester according to claim 1, characterized in that the ejector pin type telescopic mechanism (9) is located inside the cylindrical housing (1) of the harvester, and is mounted on one end of the outer wall of the small push rod (3) with the collection wire, which is close to the sealing rubber ring (4), and the large push rod (2) with the thin needle tube type structure and the small push rod (3) with the collection wire are locked in an extending state after being pushed out simultaneously by the ejector pin type telescopic mechanism (9), and are retracted to the initial position after being pressed again.
7. A subgingival anaerobic flora collector according to claim 2, characterized in that the lower end of the inner ring spring (8 a) is fixedly connected with the inner wall of the large push rod (2) with the thin needle tube-like structure, and the upper end is fixedly connected with the outer wall of the small push rod (3) with the collection wire;
the upper end of the outer ring spring (8 b) is fixedly connected with the large push rod (2) with the thin needle tube-like structure at the position of the zero scale mark (2 b), and the lower end of the outer ring spring is fixedly connected with the bottom end of the inner wall of the cylindrical shell (1) of the collector.
8. A subgingival anaerobic flora harvester according to claim 7, in which after pressing the second push plate (3 b) of the small push rod (3) with the harvesting wire, the outer ring spring (8 b) is compressed until the front needle (2 a) is completely penetrated out of the rubber membrane, and then the second push plate (3 b) is continuously pressed until the inner ring spring (8 a) is completely compressed, and the harvesting wire (5) is completely extended out of the needle (2 a); after the hands are loosened, the inner ring spring (8 a) and the outer ring spring (8 b) are respectively restored to the natural state, and the needle head (2 a) and the collection wire (5) are retracted to the initial positions.
9. A subgingival anaerobic flora harvester according to claim 8, wherein said harvesting filaments (5) have a villiated open structure, and said sealed outlet cover (6) has a 1mm thick sealant therein.
10. A method of harvesting using a subgingival anaerobic flora harvester of any one of claims 1-9, comprising the steps of:
firstly, a sealed outlet cover (6) at the front section of the collector is taken down, the body of the collector is held by hands, the outlet of a collection wire (5) is aligned with a gingival sulcus or periodontal pocket, a large push rod (2) with a thin needle tube-like structure is pushed, a needle head (2 a) is pushed out from a rubber membrane, and zero scale marks (2 b) and black scale marks (3 a) on the outer wall of the large push rod (2) with the thin needle tube-like structure are slowly exposed in the process of pushing out the needle head (2 a) by the large push rod (2) with the thin needle tube-like structure;
the needle head (2 a) is positioned by observing the exposed scale values during collection, anaerobic flora of gingival sulcus or periodontal pocket is collected when the exposed scale values reach a certain value, and the positioned needle head (2 a) is locked by the ejector pin type telescopic mechanism (9);
the small push rod (3) with the collection wire is continuously pushed, the collection wire (5) at the front end of the thin rod-shaped structure is driven to continuously extend out of the needle head (2 a) and extend into the gingival sulcus or periodontal pocket, and the depth of the collection wire extending into the sulcus or pocket is determined;
the villus structure on the collection silk (5) adsorbs the flora gathered in the ditch or the bag, and after 30 seconds, the small push rod (3) with the collection silk is pressed down by force and then loosened, the small push rod (3) with the collection silk is retracted first, then the large push rod (2) with the fine needle tube sample structure is retracted, and the outlet cover (6) is covered to complete the collection operation of the subgingival anaerobic flora.
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| CN201810652633.9A CN109009235B (en) | 2018-06-22 | 2018-06-22 | Subgingival anaerobic flora collector and use method thereof |
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| CN109009235B true CN109009235B (en) | 2023-10-13 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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