CN103505299A - Oral cavity in-site dental plaque collection device - Google Patents

Oral cavity in-site dental plaque collection device Download PDF

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Publication number
CN103505299A
CN103505299A CN201310445081.1A CN201310445081A CN103505299A CN 103505299 A CN103505299 A CN 103505299A CN 201310445081 A CN201310445081 A CN 201310445081A CN 103505299 A CN103505299 A CN 103505299A
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China
Prior art keywords
bacterial plaque
base
oral cavity
original position
catcher
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CN201310445081.1A
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Chinese (zh)
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CN103505299B (en
Inventor
梅李
周学东
赵志河
徐欣
方婕
曲雯雯
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Sichuan University
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Sichuan University
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  • Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)

Abstract

An oral cavity in-site dental plaque collection device comprises a dental plaque collection box and a base, wherein a cavity for accommodating a sample collecting material is arranged at the upper part of the dental plaque collection device, an embedded slot combined with the base is formed in the bottom of the dental plaque collection box, the upper part of the base is an embedded body matched with the embedded slot in the dental plaque collection box, the lower part of the base is a base body, the base body is connected with the embedded body through a cylinder, the embedded body on the base is inserted into the embedded slot in the collection box when the oral cavity in-site dental plaque collection device is in a use state, and a retention part is installed around the cylinder of the base. The oral cavity in-site dental plaque collection device can acquire the dental plaque with an integral three-dimensional space structure, so that the dental plaque can be collected in site.

Description

Oral cavity original position bacterial plaque catcher
Technical field
The invention belongs to Dental Plaque collection technique field, the device that particularly a kind of oral cavity original position bacterial plaque is collected.
Background technology
In oral cavity, exist natural bacteria, of a great variety, the current known 50 kinds of above different generas that have at least, it adheres to body surface formation and has aggressive, drug-fast bacterial community, is dental plaque.Dental plaque can be considered the microecological environment of antibacterial, and antibacterial grows, grows, breeds and becomes feeble and die and carries out therein complicated metabolic activity in this environment.Dental plaque can cause multiple oral disease, and as gingivitis, periodontitis, Chalk speckle, dental caries and halitosis etc., severe patient also can cause bacterial endocarditis.The correlational study of dental plaque becomes the most important thing of oral disease research undoubtedly, and obtaining of dental plaque is the primary problem solving in research work.
In existing dental plaque research work, obtain the method for dental plaque for adopting sterilized instrument scraping dental plaque sample, though can obtain dental plaque main component like this, but inevitably destroyed the original three-D space structure of dental plaque, had a strong impact on carrying out and experiment effect of experimentation.
Summary of the invention
The object of the invention is to overcome the Shortcomings of prior art, a kind of oral cavity original position bacterial plaque catcher is provided, to obtain the dental plaque that three-D space structure is complete, realize the original position of dental plaque and collect.
Oral cavity of the present invention original position bacterial plaque catcher, comprise bacterial plaque disposable box and base, described bacterial plaque disposable box top is for placing the cavity of sample collection material, bottom is provided with the embeded slot with base combination, the top of described base is the chimera mating with embeded slot in bacterial plaque disposable box, bottom is pedestal, between pedestal and chimera, by cylinder, joins;
During using state, chimera on base inserts in the embeded slot that bacterial plaque disposable box arranges, and around the cylinder of base, retention means is installed.
Above-mentioned oral cavity original position bacterial plaque catcher, the cavity of described placement sample collection material is the untight cavity in He Yi side, top.
Above-mentioned oral cavity original position bacterial plaque catcher, described bacterial plaque disposable box setting with embeded slot base combination be dovetail groove, described dovetail groove is blind-hole type dovetail groove, the chimeric shape and size of base setting and the shape and size of described dovetail groove match.
Above-mentioned oral cavity original position bacterial plaque catcher, the pedestal lower surface of described base is to recessed cambered surface, the radian of described cambered surface and the radian of facies buccalis dentis match.
Above-mentioned oral cavity original position bacterial plaque catcher, described retention means is that mouth cavity orthodontic divides tooth circle or mouth cavity orthodontic rustless steel ligature with rubber.
Above-mentioned oral cavity original position bacterial plaque catcher, its length L, width B, height h are respectively 4mm≤L≤10mm, 2mm≤B≤5mm, height 2mm≤h≤5mm.
Above-mentioned oral cavity original position bacterial plaque catcher, described base, bacterial plaque disposable box are made of the material with biocompatibility, preferably of rustless steel, make.The preferred global formation of manufacture method, the chimera, pedestal, the cylinder that make to form base are integrated, the each several part one-shot forming of bacterial plaque disposable box.
Above-mentioned oral cavity original position bacterial plaque catcher, the edge of described base, bacterial plaque disposable box need pass through processed, makes its smooth circle blunt, oral cavity is caused damage avoiding.
Oral cavity original position bacterial plaque catcher of the present invention using method in clinical experiment is as follows:
By the required sample collection material of experimentation (as hydroxy-apatite flag, enamel sheet etc.) with not being adhered in the cavity of placement sample collection material of bacterial plaque disposable box containing the bonding agent of antibacterial bacteriostatic composition, then the chimera on base is inserted in the embeded slot of bacterial plaque disposable box bottom setting, cylinder by retention means around base, base and bacterial plaque collecting cassette are fixed together, continue after by animal or human's body oral cavity routine every wet, the phosphoric acid acid etching facing to be sampled that is 37% by the volumetric concentration that oral cavity is used, with orthodontic bracket bonding agent by the buccal surface of the cambered surface of bacterial plaque catcher base and tooth bonding (as shown in Figure 9), complete the installation of bacterial plaque catcher of the present invention and sample collection material.
According to different experiments, design, if will again collect bacterial plaque, first in oral cavity, retention means is taken off, again the bacterial plaque disposable box that obtains bacterial plaque to be measured is taken off, then as stated above will be bonded with new bacterial plaque disposable box and the base combination and fixing of sample collection material, the bacterial plaque disposable box that only needs to change bacterial plaque catcher can be carried out original position bacterial plaque next time and be collected.
Tested Hou,Jiang oral cavity original position bacterial plaque catcher integral body and taken off from facing, if facing residual fraction bonding agent, available rubber cup etc. are removed bonding agent, polishing facing.
Compared with prior art, the present invention has following beneficial effect:
1, because oral cavity of the present invention original position bacterial plaque catcher can be fixed on human or animal's tooth, make oral plaque self-assembling formation and grow on the sample collection material in bacterial plaque disposable box, realizing the original position of bacterial plaque collects, thereby can obtain the dental plaque of the complete and self-sow form of three-D space structure, for the research of composition, three-D space structure and the follow-up intervention prophylactico-therapeutic measures etc. of Dental Plaque provides original reliable object of study.
2, because the base of oral cavity of the present invention original position bacterial plaque catcher and bacterial plaque disposable box are for removably connecting, when need repeatedly collecting bacterial plaque, experimentation only need take off the bacterial plaque disposable box that has obtained bacterial plaque to be measured, to after the bonding sample collection material of new bacterial plaque disposable box, collect with the fixing bacterial plaque next time of can carrying out of intraoral base, thereby not only reduced installation procedure, and can reduce use cost because of reusing of base.
3, oral cavity of the present invention original position bacterial plaque accumulator volumes is little, and the smooth of the edge circle is blunt, and has biocompatibility, thereby can be to not causing any interference animal or human's orthobiosis.
4, oral cavity of the present invention original position bacterial plaque collector structure is simple, is convenient to processing and fabricating.
Accompanying drawing explanation
Fig. 1 is the front view of the bacterial plaque disposable box of oral cavity of the present invention original position bacterial plaque catcher;
Fig. 2 is the top view of Fig. 1;
Fig. 3 is the left view of Fig. 1;
Fig. 4 is the front view of the base of oral cavity of the present invention original position bacterial plaque catcher;
Fig. 5 is left view or the right view of Fig. 4;
Fig. 6 is the front view of oral cavity of the present invention original position bacterial plaque catcher;
Fig. 7 is the left view of Fig. 6;
Fig. 8 is the right view of Fig. 6;
Fig. 9 is the using state figure of oral cavity of the present invention original position bacterial plaque catcher.
In figure, the cavity of 1-bacterial plaque disposable box, 11-placement sample collection material, 12-embeded slot, 2-base, 21-pedestal, 22-cylinder, 23-chimera, 3-retention means, 4-tooth, 5-oral cavity original position bacterial plaque catcher.
The specific embodiment
Below by embodiment, oral cavity of the present invention original position bacterial plaque catcher is described further.
Embodiment 1
In the present embodiment, the structure of described oral cavity original position bacterial plaque catcher, as shown in Fig. 1~8, comprises bacterial plaque disposable box 1 and base 2.Described bacterial plaque disposable box 1 top is the cavity 11 of the untight placement sample collection in He Yi side, top material, and bottom is provided with the embeded slot 12 with base combination, and described embeded slot is blind-hole type dovetail groove.The top of described base 2 for bacterial plaque disposable box on dovetail groove shape and the chimera 23 that matches of size, bottom is pedestal 21, pedestal lower surface is to recessed cambered surface, and the radian of this cambered surface and the radian of facies buccalis dentis match, and between pedestal 21 and chimera 23, by cylinder 22, joins.The material of bacterial plaque disposable box 1 and base 2 is rustless steel, respectively by casting global formation.
During using state, chimera 23 on base 2 inserts in the embeded slot 12 of bacterial plaque disposable box 1 setting, cylinder 22 around base is provided with retention means 3, described retention means is that mouth cavity orthodontic divides tooth circle with rubber, the length L of the oral cavity original position bacterial plaque catcher combining is 10mm, width B is 5mm, and height h is 5mm.
Embodiment 2
In the present embodiment, the difference of described oral cavity original position bacterial plaque catcher and embodiment 1 is: described retention means 3 is the rustless steel ligature of mouth cavity orthodontic with 0.22mm.The length L of the oral cavity original position bacterial plaque catcher combining is 4mm, and width B is 2mm, and height h is 2mm.

Claims (10)

1.Yi Zhong oral cavity original position bacterial plaque catcher, it is characterized in that comprising bacterial plaque disposable box (1) and base (2), described bacterial plaque disposable box (1) top is for placing the cavity (11) of sample collection material, bottom is provided with the embeded slot (12) with base combination, the top of described base (2) is the chimera (23) mating with embeded slot in bacterial plaque disposable box, bottom is pedestal (21), between pedestal (21) and chimera (23), by cylinder (22), joins;
During using state, chimera on base (2) inserts in the embeded slot (12) that bacterial plaque disposable box (1) arranges, and around the cylinder (22) of base, retention means (3) is installed.
2. oral cavity original position bacterial plaque catcher according to claim 1, the cavity (11) that it is characterized in that placing sample collection material is the untight cavity in He Yi side, top.
3. according to oral cavity original position bacterial plaque catcher described in claim 1 or 2, it is characterized in that the embeded slot (12) with base combination that bacterial plaque disposable box (1) arranges is dovetail groove, described dovetail groove is blind-hole type dovetail groove, and the shape and size of the chimera (23) that base (2) arranges and the shape and size of described dovetail groove match.
4. according to oral cavity original position bacterial plaque catcher described in claim 1 or 2, the pedestal lower surface that it is characterized in that base (2) is that the radian of described cambered surface and the radian of facies buccalis dentis match to recessed cambered surface.
5. oral cavity original position bacterial plaque catcher according to claim 3, the pedestal lower surface that it is characterized in that base (2) is for to recessed cambered surface, and the radian of described cambered surface and the radian of facies buccalis dentis match.
6. according to oral cavity original position bacterial plaque catcher described in claim 1 or 2, it is characterized in that retention means (3) divides tooth circle or mouth cavity orthodontic rustless steel ligature for mouth cavity orthodontic with rubber.
7. oral cavity original position bacterial plaque catcher according to claim 3, is characterized in that retention means (3) divides tooth circle or mouth cavity orthodontic rustless steel ligature for mouth cavity orthodontic with rubber.
8. oral cavity original position bacterial plaque catcher according to claim 4, is characterized in that retention means (3) divides tooth circle or mouth cavity orthodontic rustless steel ligature for mouth cavity orthodontic with rubber.
9. according to oral cavity original position bacterial plaque catcher described in claim 1 or 2, it is characterized in that chimera (23), pedestal (21) and the cylinder (22) of base is integrated.
10. according to oral cavity original position bacterial plaque catcher described in claim 1 or 2, it is characterized in that its length L, width B, height h is respectively 4mm≤L≤10mm, 2mm≤B≤5mm, height 2mm≤h≤5mm.
CN201310445081.1A 2013-09-26 2013-09-26 Oral cavity in-site dental plaque collection device Expired - Fee Related CN103505299B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105455856A (en) * 2015-12-26 2016-04-06 浙江大学 In-situ plaque biological membrane intraoral model and preparation method thereof
CN109009235A (en) * 2018-06-22 2018-12-18 四川大学 Anaerobic bacteria flora collector and application method under a kind of gum

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JP2003250515A (en) * 2002-03-01 2003-09-09 Ngk Insulators Ltd Reaction cell and method for using the same
US20070166819A1 (en) * 2006-01-16 2007-07-19 Reliance Life Sciences Pvt. Ltd. Device for culturing and transporting cells
CN202089986U (en) * 2011-06-10 2011-12-28 朴美花 Disposable fungus culture vessel

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FR2777903B1 (en) * 1998-04-24 2000-12-29 Millipore Sa METHOD FOR DETECTION OF MICROORGANISMS AND CASSETTE SUITABLE FOR IMPLEMENTING IT
FR2915487B1 (en) * 2007-04-26 2009-06-05 Millipore Corp ASSEMBLY AND METHOD FOR MICROBIOLOGICAL ANALYSIS
CN202744549U (en) * 2012-05-25 2013-02-20 曾勇 Automatic solution replacing embryo culture dish
CN203048954U (en) * 2013-02-05 2013-07-10 齐齐哈尔大学 Biological cell culturing apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003250515A (en) * 2002-03-01 2003-09-09 Ngk Insulators Ltd Reaction cell and method for using the same
US20070166819A1 (en) * 2006-01-16 2007-07-19 Reliance Life Sciences Pvt. Ltd. Device for culturing and transporting cells
CN202089986U (en) * 2011-06-10 2011-12-28 朴美花 Disposable fungus culture vessel

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105455856A (en) * 2015-12-26 2016-04-06 浙江大学 In-situ plaque biological membrane intraoral model and preparation method thereof
CN105455856B (en) * 2015-12-26 2018-01-26 浙江大学 Plaque bio-film intraoral model in situ and preparation method thereof
CN109009235A (en) * 2018-06-22 2018-12-18 四川大学 Anaerobic bacteria flora collector and application method under a kind of gum
CN109009235B (en) * 2018-06-22 2023-10-13 四川大学 Subgingival anaerobic flora collector and use method thereof

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