CN215480983U - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- CN215480983U CN215480983U CN202121777844.9U CN202121777844U CN215480983U CN 215480983 U CN215480983 U CN 215480983U CN 202121777844 U CN202121777844 U CN 202121777844U CN 215480983 U CN215480983 U CN 215480983U
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Abstract
The utility model discloses a cell culture device. The cell culture device comprises a culture medium storage dish and a movable culture dish movably sleeved in the culture medium storage dish, and comprises a porous part and a non-porous part, wherein the porous part is positioned in the middle of the side wall. The cell culture device disclosed by the utility model obviously improves the efficiency of liquid change and exosome collection during cell culture, brings convenience for extraction of single-hole cells, discrimination of target cells during co-culture and the like, and has simpler working procedures, purer results and higher efficiency.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. Cell culture techniques are important and commonly used in cell biology research methods, and can be used for obtaining a large number of cells through cell culture and researching signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells, induced differentiation of stem cells, collection of exosomes and the like. At present, a culture dish with a plurality of culture grooves is often adopted for cell culture, the culture grooves are mutually independent, and when a large amount of cells are proliferated or differentiated and need to be changed, the steps of cell centrifugation or cell transfer are usually included, and the operation is complex.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the utility model provides a cell culture device which can realize quick liquid change and is simple and convenient to operate.
In a first aspect of the present invention, a cell culture apparatus is provided, which includes a culture medium storage dish and a movable culture dish movably sleeved in the culture medium storage dish, wherein a side wall of the movable culture dish includes a porous portion and a non-porous portion, and the porous portion is located in a middle portion of the side wall.
According to the cell culture device provided by the embodiment of the utility model, at least the following beneficial effects are achieved: the culture medium storage dish and the movable culture dish are matched, namely, one culture medium storage dish is matched with one movable culture dish, and the culture medium storage dish is applicable to culture of various cells. The specific process can be as follows: (1) when the cells are subjected to suspension culture, the culture medium storage dish and the movable culture dish are combined, the cells are planted in the movable culture dish, and when liquid is changed or cell exosomes are collected, a pipette can be directly adopted to suck along one side of the wall of the culture medium storage dish, or the movable culture dish is lifted out and placed in another clean culture medium storage dish, so that the method is simple and convenient, the cells do not need to be centrifuged to re-suspend and change the liquid, the damage and loss of the cells are reduced, the time and experimental consumables are saved, and the cost is saved; (2) when cells need co-culture, non-target cells can be planted in the culture medium storage dish, target cells are planted in the movable culture dish for culture, and if the target cells need to be collected, the movable culture dish only needs to be directly taken out.
Different from the traditional operation, the device obviously improves the collection efficiency of liquid changing and exosome during cell culture, brings convenience for the extraction of single-hole cells, the discrimination of target cells during co-culture and the like, and has simpler working procedures, purer results and higher efficiency. Meanwhile, if the cell growth or differentiation state needs to be observed in stages, one or more movable culture dishes can be directly taken away, the operation is convenient and fast, the pollution probability is reduced, the cost is reduced, great convenience is brought to scientific research personnel through the design, and the time is saved.
The culture medium storage dish and the movable culture dish in the utility model can be customized according to needs, and can also be obtained by modifying a multi-hole culture dish in the existing market (such as the existing model 6, 12, 24 and 48-hole culture dishes in the market).
In some embodiments of the utility model, the porous portion and the non-porous portion are of different materials.
In some embodiments of the present invention, the surface of the porous portion is provided with an anti-blocking layer.
In the above embodiment, in order to provide the porous portion with an anti-sticking function and prevent the porous portion from being blocked by excessive sticking of cells, an anti-blocking layer may be provided on the surface of the porous portion, and specifically, the anti-blocking layer may be applied to the surface of the porous portion.
In some embodiments of the utility model, the perforated section is a screen structure.
The filter screen structure has the function of anti-sticking, prevents the cells from excessively sticking to block the filter screen, and can be made of nylon. Meanwhile, the pore size of the filter screen structure can be set according to actual requirements, for example, when cell suspension culture is carried out, the filter screen structure only allows non-cell substances to pass between the cell culture dish and the culture medium storage dish; when the induction culture of the pluripotent stem cells to form embryoid bodies is carried out, the cell culture dish with the filter screen with larger pore diameter can be selected to remove the cells with small cell size or the cells with small sphere.
In some preferred embodiments of the utility model, the screen structure is a first cell screen.
Through above-mentioned embodiment, first cell filter screen can be the cell sieve, according to actual need, sets up the aperture size of first cell filter screen.
In some preferred embodiments of the present invention, the mesh structure has a pore size in the range of 10 to 100 μm.
In some preferred embodiments of the utility model, the screen structure is made of nylon.
In some embodiments of the utility model, the removable culture dish is made of polystyrene or polycarbonate.
In some preferred embodiments of the present invention, the movable culture dish has an upper opening, and the top of the strainer structure is connected to a periphery of the upper opening of the movable culture dish.
In some preferred embodiments of the utility model, the bottom of the sieve structure is at a set distance from the bottom of the movable culture dish.
Through above-mentioned embodiment, set distance has between the bottom of filter screen structure and the bottom of activity culture dish, specifically means: the bottom of the filter screen structure is not directly and completely attached to the bottom of the movable culture dish, and a certain gap is formed.
In some more preferred embodiments of the utility model, the distance between the bottom of the sieve structure and the bottom of the movable culture dish is about 0.5 cm.
Through above-mentioned embodiment, the upper end opening of activity culture dish, the bottom is solid, begins from activity culture dish bottom and sets up the solid wall of 0.5cm high about from bottom to top, and remaining lateral wall is the filter screen structure. The pore size of the filter screen structure can be customized according to the cell size (10-100 μm), so that the cells are effectively prevented from falling into the culture medium storage dish.
In some embodiments of the utility model, the culture device further comprises a connecting plate, wherein a plurality of movable culture dishes are arranged, and two adjacent movable culture dishes are connected through the connecting plate.
Through above-mentioned embodiment, a plurality of continuous activity culture dishes are convenient for get a plurality of activity culture dishes simultaneously and trade liquid or observe the detection, improve scientific research personnel work efficiency. If the cell growth or differentiation state needs to be observed in stages, a plurality of movable culture dishes are directly taken away, the operation is convenient and fast, the probability of pollution is reduced, the cost is reduced, great convenience is brought to scientific research personnel, and the time is saved. The material of the connecting plate may be the same as that of the movable culture dish.
In some preferred embodiments of the present invention, the connection plate is disposed at a periphery of an upper end opening of the movable culture dish.
In some further preferred embodiments of the utility model, the bottom of the connection plate abuts against the upper end of the medium storage dish when the removable culture dish is positioned in the medium storage dish.
In some more preferred embodiments of the present invention, a plurality of the movable culture dishes are distributed in an array by a connecting plate, and the movable culture dishes at the end parts are provided with supporting ears.
In some more preferred embodiments of the present invention, a plurality of the movable culture dishes are arranged in a row by a connecting plate, and the movable culture dishes at the end are provided with supporting ears.
Through above-mentioned embodiment, can be as required with vertical or horizontal movable culture dish one-piece even, support the ear and set up on two movable culture dishes at the both ends of one row of movable culture dish, support the ear and not only can cooperate the culture medium to deposit the effect that the dish played the support to movable culture dish, support on culture medium deposits the dish or on other bearing structure, conveniently draw the movable culture dish in row moreover, reasonable in design to in the operation. The sizes of the movable culture dish and the culture medium storage dish can be consistent with the sizes and specifications of holes in the multi-hole culture dish in the market, and the movable culture dish is required to be sleeved in the culture medium storage dish.
In some more preferred embodiments of the present invention, a plurality of the culture medium storage dishes are provided, and each of the movable culture dishes is respectively sleeved in a single culture medium storage dish.
Through above-mentioned embodiment, as required, deposit the nimble subassembly that constitutes of ware and activity culture dish with the culture medium. The culture medium storage dish and the movable culture dish can be separated and combined with each other by utilizing a modular design, and are integrated after combination, the liquid change is different from the traditional operation (for example, suction is respectively carried out on one hole and one hole), the operation is simpler and more convenient, and particularly, the culture suspension culture liquid change is more convenient, the centrifugation is not needed any more, and the waste liquid can be directly transferred; and when the cell growth or differentiation state needs to be observed stage by stage, one or more movable culture dishes are directly taken away, so that the method is convenient and fast, the pollution probability is reduced, the cost is reduced, great convenience is brought to scientific research personnel, and the time is saved.
In some more preferred embodiments of the present invention, the support ears are provided at the periphery of the upper end opening of the movable culture dish.
In some embodiments of the utility model, the bottom of the medium reservoir plate is a set distance from the bottom of the removable culture plate.
Through the above embodiment, the distance between the bottom of the culture medium storage dish and the bottom of the movable culture dish is specifically: the bottom of the culture medium storage dish and the bottom of the movable culture dish are not directly and completely attached to each other, and a certain gap is formed between the bottom of the culture medium storage dish and the bottom of the movable culture dish, so that the movable culture dish and the culture medium storage dish can conveniently circulate substances and the cells in the culture medium storage dish can conveniently grow like cell co-culture.
In some preferred embodiments of the utility model, the bottom of the inner wall of the medium reservoir dish is at a set distance a > 0 from the bottom of the outer wall of the movable culture dish.
In some more preferred embodiments of the utility model, the distance between the bottom of the inner wall of the medium reservoir dish and the bottom of the outer wall of the mobile culture dish is about 1.5 mm.
In some embodiments of the utility model, the side wall of the medium reservoir plate is at a set distance from the side wall of the removable culture plate.
Through the above embodiment, the distance between the side wall of the culture medium storage dish and the side wall of the movable culture dish is specifically: the side wall of the culture medium storage dish and the side wall of the movable culture dish which are arranged in a set are not directly and completely attached, and a certain gap is formed, so that the material circulation between the movable culture dish and the culture medium storage dish is convenient for the cell growth in the culture medium storage dish during cell co-culture.
In some preferred embodiments of the utility model, the inner side wall of the medium storage dish is at a set distance b > 0 from the outer side wall of the movable culture dish.
In some more preferred embodiments of the utility model, the distance between the inner side wall of the medium reservoir vessel and the outer side wall of the mobile culture vessel is about 1 mm.
In some preferred embodiments of the utility model, the bottom of the medium storage dish is a set distance a, a > 0 from the bottom of the removable culture dish, the side wall of the medium storage dish is a set distance b, b > 0 from the side wall of the removable culture dish, and a and b are equal or different.
In some embodiments of the utility model, the diameter of the removable culture dish is 22mm and the diameter of the medium storage dish is 23 mm.
In some embodiments of the utility model, a plurality of the culture medium storage dishes are arranged, and the plurality of the culture medium storage dishes are communicated through the communication pipeline.
Through above-mentioned embodiment, the setting of intercommunication pipeline is convenient for a plurality of culture medium storage dishes and is unified to trade liquid or remove, the operation of being convenient for.
In some preferred embodiments of the present invention, the communication channel includes a plurality of connection pipes, and two adjacent culture medium storage dishes are communicated through the connection pipes, and two ends of the connection pipes are provided with a net structure.
Through the above embodiment, the connection pipe and the net structure are arranged and matched with each other, so that the material circulation of the culture medium storage dish is realized, and the circulated material is limited and selected due to the arrangement of the net structure. Wherein, the material of connecting pipe can be the same with the material of culture medium storage dish.
Thereby two adjacent culture medium deposit the ware and have network structure's connecting pipe intercommunication to form the integral type with both ends to network structure can select nylon material, prevents that the cell from excessively being stained with to glue and plugging up the filter screen, when the cell needs coculture, can deposit the culture in the ware with non-target cell kind at the culture medium.
In some more preferred embodiments of the utility model, the mesh structure is a second cell strainer.
Through above-mentioned embodiment, the second cell filter screen can be the cell sieve, according to actual need, sets up the aperture size of second cell filter screen.
In some more preferred embodiments of the present invention, the mesh structure has a pore size in the range of 10 to 100 μm.
In some more preferred embodiments of the utility model, the mesh structure is made of nylon.
In some embodiments of the utility model, the medium holding dish is made of polystyrene or polycarbonate.
In some preferred embodiments of the present invention, a liquid introducing groove is further included, and the liquid introducing groove is communicated with the communication pipeline.
Through above-mentioned embodiment, draw the cistern and communicate with the communicating pipe, be convenient for trade liquid or to the culture medium liquid feeding in the storage dish or from the culture medium imbibition in the storage dish, reasonable in design, easy operation.
In some embodiments of the utility model, a base is further included, and the medium storage dish is disposed within the base.
In some preferred embodiments of the present invention, the plurality of movable culture dishes are connected in an array by a connecting plate, the movable culture dishes at the ends are provided with supporting lugs, the supporting lugs are arranged on the periphery of the upper end opening of the movable culture dishes, and the supporting lugs abut against the top of the base when the movable culture dishes are respectively located in the culture medium storage dish.
In some preferred embodiments of the present invention, the culture dish further comprises a culture dish cover, and the culture dish cover is installed in cooperation with the base.
In some preferred embodiments of the present invention, the culture dish further comprises a culture dish cover, and the culture dish cover is covered on the base.
In some more preferred embodiments of the present invention, the side wall of the base is stepped, and the culture dish cover covers the stepped layer in the middle of the base.
In some further preferred embodiments of the utility model, a side of the base adjacent to the medium reservoir vessel is higher than a side of the base remote from the medium reservoir vessel.
Drawings
The utility model is further described with reference to the following figures and examples, in which:
FIG. 1 is a schematic view showing the structure of a cell culture apparatus according to an embodiment of the present invention;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is a first schematic view of the structure of the removable culture dish part of the cell culture apparatus according to the embodiment of the present invention;
FIG. 4 is a second schematic structural view of a removable culture dish portion of the cell culture apparatus according to the embodiment of the present invention;
FIG. 5 is a first schematic view showing the structure of a medium storing dish part of the cell culture apparatus according to the embodiment of the present invention;
FIG. 6 is a second schematic view showing the structure of a culture medium storing dish part of the cell culture apparatus according to the embodiment of the present invention.
Reference numerals: 1. a culture medium storage dish; 11. a network structure; 2. a movable culture dish; 21. a screen structure; 22. a connecting plate; 23. a support ear; 3. a connecting pipe; 4. a liquid drainage tank; 5. a base; 6. and (5) closing the culture dish.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality is one or more, the meaning of a plurality is two or more, and the above, below, exceeding, etc. are understood as excluding the present numbers, and the above, below, within, etc. are understood as including the present numbers. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
In the description of the present invention, reference to the description of the terms "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Example 1
This example discloses a cell culture apparatus, as shown in FIGS. 1-6: deposit the movable culture dish 2 in dish 1 is deposited to culture medium including culture medium storage dish 1 and movable sleeve, and the lateral wall of movable culture dish 2 includes porose part and sclausura part, porose part is located the middle part of lateral wall, porose part are filter screen structure 21, and filter screen structure 21 is first cell filter screen, and filter screen structure 21's aperture scope is 10-100 mu m.
2 upper end openings of activity culture dish, filter screen structure 21 set up in the lateral wall of activity culture dish 2, and filter screen structure 21's top links to each other with the upper end open-ended periphery of activity culture dish 2, and the distance between the bottom of filter screen structure 21 and the bottom of activity culture dish 2 is 0.5 cm.
The cell culture device further comprises a connecting plate 22, the movable culture dishes 2 are arranged in a plurality of numbers, two adjacent movable culture dishes 2 are connected through the connecting plate 22, the connecting plate 22 is arranged on the periphery of an upper end opening of each movable culture dish 2, and when the movable culture dishes 2 are located in the culture medium storage dish 1, the bottom of the connecting plate 22 is abutted to the upper end of the culture medium storage dish 1. The plurality of movable culture dishes 2 are distributed in an array mode (specifically, distributed in an array mode) through the connecting plate 22, the movable culture dishes 2 positioned at the end parts are provided with supporting lugs 23, and the supporting lugs 23 are arranged on the periphery of an upper end opening of the movable culture dishes 2. The culture medium storage dish 1 is provided with a plurality of, and every activity culture dish 2 overlaps respectively to be located in single culture medium storage dish 1.
The distance between the bottom of the inner wall of the medium storage dish 1 and the bottom of the outer wall of the movable culture dish 2 is 1.5 mm.
The distance between the inner side wall of the culture medium storage dish 1 and the outer side wall of the movable culture dish 2 is 1 mm.
The cell culture device also comprises a communicating pipeline, and the culture medium storage dishes 1 are communicated with each other through the communicating pipeline. The communicating pipe comprises a plurality of connecting pipes 3, two adjacent culture medium storage dishes 1 are communicated through the connecting pipes 3, and two ends of each connecting pipe 3 are provided with a net-shaped structure 11. The mesh structure 11 is a second cell strainer. The pore size of the network 11 is in the range of 10-100 μm.
The cell culture device also comprises a liquid guiding groove 4, and the liquid guiding groove 4 is communicated with the communicating pipeline.
The cell culture device also comprises a base 5, and the culture medium storage dish 1 is arranged in the base 5; when the movable culture dish 2 is respectively positioned in the culture medium storage dish 1, the supporting lug 23 is abutted against the top of the base 5.
The cell culture device also comprises a culture dish cover 6, and the culture dish cover 6 is matched with the base 5. The culture dish cover 6 covers the base 5. The side wall of the base 5 is step-shaped, and the culture dish cover 6 covers the step layer surface in the middle of the base 5. The side of the base 5 close to the culture medium storage dish 1 is higher than the side of the base 5 far from the culture medium storage dish 1.
Wherein, the filter screen structure 21 and the net structure 11 are both made of nylon, and the movable culture dish 2 and the culture medium storage dish 1 are both made of polystyrene. The filter structure 21 and the mesh structure 11 have anti-adhesion function to prevent the filter from being blocked by the cells.
According to the cell culture device provided by the embodiment of the utility model, the culture medium storage dish and the movable culture dish are arranged in a matched manner, namely one culture medium storage dish is matched with one movable culture dish, so that the cell culture device is suitable for culturing various cells. The specific process can be as follows: (1) when the cells are subjected to suspension culture, the culture medium storage dish and the movable culture dish are combined, the cells are planted in the movable culture dish, and when liquid is changed or cell exosomes are collected, a pipette can be directly adopted to suck along one side of the wall of the culture medium storage dish, or the movable culture dish is lifted out and placed in another clean culture medium storage dish, so that the method is simple and convenient, the cells do not need to be centrifuged to re-suspend and change the liquid, the damage and loss of the cells are reduced, the time and experimental consumables are saved, and the cost is saved; (2) when cells need co-culture, non-target cells can be planted in the culture medium storage dish, target cells are planted in the movable culture dish for culture, and if the target cells need to be collected, the movable culture dish only needs to be directly taken out.
Different from the traditional operation, the device obviously improves the collection efficiency of liquid changing and exosome during cell culture, brings convenience for the extraction of single-hole cells, the discrimination of target cells during co-culture and the like, and has simpler working procedures, purer results and higher efficiency. Meanwhile, if the cell growth or differentiation state needs to be observed in stages, one or more movable culture dishes can be directly taken away, the operation is convenient and fast, the pollution probability is reduced, the cost is reduced, great convenience is brought to scientific research personnel through the design, and the time is saved.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.
Claims (10)
1. The cell culture device is characterized by comprising a culture medium storage dish and a movable culture dish, wherein the movable culture dish is movably sleeved in the culture medium storage dish, the side wall of the movable culture dish comprises a porous part and a non-porous part, and the porous part is positioned in the middle of the side wall.
2. The cell culture apparatus according to claim 1, wherein the porous portion and the non-porous portion are made of different materials.
3. The cell culture device according to claim 2, further comprising a plurality of the movable culture dishes, wherein two adjacent movable culture dishes are connected by the connecting plate.
4. The cell culture apparatus according to claim 3, wherein a plurality of the movable culture dishes are arranged in an array by a connecting plate, and the movable culture dishes at the end parts are provided with supporting lugs.
5. A cell culture apparatus according to claim 1, wherein the bottom of the medium storage vessel is a predetermined distance a, a > 0 from the bottom of the movable vessel, the side walls of the medium storage vessel are a predetermined distance b, b > 0 from the side walls of the movable vessel, and a and b are equal or different.
6. The cell culture apparatus according to claim 1, further comprising a plurality of communication channels, wherein the plurality of medium storage dishes are communicated with each other through the communication channels.
7. The cell culture apparatus according to claim 6, wherein the communication channel comprises a plurality of connection pipes, two adjacent culture medium storage dishes are communicated through the connection pipes, and two ends of the connection pipes are provided with a net structure.
8. The cell culture apparatus according to claim 6, further comprising a liquid introducing groove, the liquid introducing groove communicating with the communicating channel.
9. The cell culture apparatus of claim 1, further comprising a base, wherein the medium reservoir is disposed within the base.
10. The cell culture device according to claim 9, further comprising a culture dish lid, wherein the culture dish lid is installed in cooperation with the base.
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| CN114317256A (en) * | 2022-02-20 | 2022-04-12 | 重庆医科大学 | Medical science is culture dish for microbiology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114317256A (en) * | 2022-02-20 | 2022-04-12 | 重庆医科大学 | Medical science is culture dish for microbiology |
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