CN208218841U - A kind of high throughput Biocontrol microorganism screening plant - Google Patents
A kind of high throughput Biocontrol microorganism screening plant Download PDFInfo
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- CN208218841U CN208218841U CN201820552651.5U CN201820552651U CN208218841U CN 208218841 U CN208218841 U CN 208218841U CN 201820552651 U CN201820552651 U CN 201820552651U CN 208218841 U CN208218841 U CN 208218841U
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- culture dish
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- screening plant
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Abstract
The utility model discloses a kind of high-throughput Biocontrol microorganism screening plants, including culture dish, culture dish is equipped with ware lid, it further include the inoculation cup being vertically arranged in culture dish, being inoculated with cup includes the consistent main cup of height and sub-cup, the main cup is detachably connected with culture dish bottom, main cup outer wall top is equipped with a ring annular groove, change is equipped in annular groove, change outer wall is fixed with multiple horizon bars equally distributed in a ring, slot is equipped in the end of horizon bar, sub-cup is that multiple and sub-cup outer walls are horizontally fixed with connecting rod, and connecting rod is matched with slot；The outer wall of culture dish, main cup and sub-cup is equipped with graduation mark.Compared with prior art, the utility model is easy to operate, easily controllable inoculation cup placement position and is not easy to turn upside down, and especially can obviously accelerate test process to handling more test with a variety of Biocontrol microorganism ferment filtrates of single treatment；And sub-cup can be rotated around main cup, select sub-cup more convenient.
Description
Technical field
The utility model relates to Biocontrol microorganism ferment filtrate secondary screening experimental field, the especially micro- lifes of a kind of high-throughput biological and ecological methods to prevent plant disease, pests, and erosion
Object screening plant.
Background technique
Culture dish is common consumptive material in the screening of biocontrol microorganisms laboratory experiment, in the experiment of biocontrol microorganisms ferment filtrate secondary screening, often
Method has filter paper enzyme, agar diffusion method and Odontothrips loti.Regardless of any method, it is typically necessary first at culture dish bottom
Portion prepares the culture medium containing pathogenic microorganism spore using coating or mixing method, and filter paper is then put on culture medium, is dug
Hole or put Oxford cup, then by Biocontrol microorganism ferment filtrate drop to be screened on filter paper in adding hole or
It is added in Oxford cup, after static 10-20min, covers ware lid, be put into insulating box and cultivate, according to the size of inhibition zone after 3-5d
It is screened and is evaluated.But it is found in test, the ferment filtrate finite volume being added in filter paper enzyme, and be easy around
It overflows；Borehole operation difficulty is big in agar diffusion method, and aperture is inconsistent, and also duration wears culture medium digging；Though Odontothrips loti
So without borehole, can the amount of increasing volume ferment filtrate, but need oneself placement position, and in culture dish moving process
In be easy tipping.
Summary of the invention
The purpose of this utility model is the deficiencies in the prior art to be solved, and provides a kind of high-throughput Biocontrol microorganism
Screening plant.
In order to achieve the above objectives, the utility model is implemented according to following technical scheme:
A kind of high throughput Biocontrol microorganism screening plant, including culture dish, the culture dish are equipped with ware lid, further include vertical
The inoculation cup being set in culture dish, the inoculation cup include opening and the consistent main cup of height and sub-cup up and down, the master
Cup is detachably connected with culture dish bottom, and the main cup outer wall top is equipped with a ring annular groove, is equipped with change in annular groove, institute
It states change outer wall and is fixed with multiple horizon bars equally distributed in a ring, be equipped with slot, the pair in the end of the horizon bar
Cup is that multiple and sub-cup outer walls are horizontally fixed with connecting rod, and the connecting rod is matched with slot；The culture dish, main cup and
The outer wall of sub-cup is equipped with graduation mark.In the technical solution, main cup can be installed or removed quickly and easily in culture dish, also
Multiple sub-cups can be quickly connected by the horizon bar in the change on main cup, and sub-cup can also pass through the change of main cup outer wall
It is rotated around main cup.
Further technical solution is, the culture dish bottom is fixed with cushion block, the cushion block upper surface open up it is fluted,
The bottom of the main cup is fixed with and the pin for being inserted into groove.It so, it is possible to facilitate and main cup be fixed in culture dish, make
After, also facilitates and take out main cup.
Further technical solution is that the slot of the horizontal boom end is rectangular channel.So, it is possible to prevent sub-cup around
Connecting rod radial rotating causes sub-cup to overturn.
Further technical solution is that the pin in the main bottom of a cup portion is set as two or more.It so, it is possible to make
Main cup, which is placed in culture dish, more to be consolidated, in addition, can prevent main cup from also and then rotating when rotating sub-cup.
Compared with prior art, the utility model is easy to operate, easily controllable inoculation cup placement position and is not easy to turn upside down, can
With a variety of Biocontrol microorganism ferment filtrates of single treatment, test especially more to processing can obviously accelerate test process；And
Sub-cup can be rotated around main cup, select sub-cup more convenient.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the main cup of the utility model and the connection schematic diagram of sub-cup.
Fig. 3 is the horizon bar of the utility model and the connection schematic diagram of connecting rod.
Specific embodiment
The utility model will be further described below with reference to the accompanying drawings and specific embodiments, in the signal of this utility model
Property embodiment and explanation be used to explain the utility model, but be not intended to limit the scope of the present invention.
Embodiment 1
As shown in Figure 1, a kind of high-throughput Biocontrol microorganism screening plant of the present embodiment, including culture dish 1, the culture
Ware 1 is equipped with ware lid 2, further includes the inoculation cup being vertically arranged in culture dish, and the inoculation cup includes equal opening and highly up and down
Consistent main cup 4 and sub-cup 6, the main cup 4 are detachably connected with 1 bottom of culture dish, and the main 4 outer wall top of cup is equipped with a circle
Annular groove 7 is equipped with change 8 in annular groove 7, and 8 outer wall of change is fixed with multiple horizon bars 9 equally distributed in a ring,
Slot 91 is equipped in the end of the horizon bar 9, the sub-cup 6 is that multiple and sub-cup 6 outer walls are horizontally fixed with connecting rod 10,
The connecting rod 10 is matched with slot 91；The outer wall of the culture dish 1, main cup 4 and sub-cup 6 is equipped with graduation mark 5.Such as Fig. 1
In, 1 bottom of culture dish is fixed with cushion block 3, and 3 upper surface of cushion block opens up fluted 31, and the bottom of the main cup 4 is fixed
Having can quickly and easily install or remove in culture dish, can also pass through with the pin 41 for being inserted into groove 31, main cup 4
The horizon bar 9 in change 8 on main cup 4 quickly connects multiple sub-cups 6, and sub-cup 6 can also pass through the change 8 of main 4 outer wall of cup
Rotated around main cup 4, facilitate according to the demand of test (such as strain fermentation filtrate type is more, when ferment filtrate concentration gradient is arranged,
Or when ferment filtrate access amount difference) carry out the use number of flexible choice sub-cup 6, selection diagram is as shown in figure 3, and rise solid
It is set for using, different numbers can also be indicated on the outer wall in sub-cup 6, as sub-cup 6 is set as eight in Fig. 3, and compile respectively
It number is that (1), (2), (3), (4), (5), (6), (7), (8) for record the group number of test make it be not easy confusion, rotation sub-cup 6
The sub-cup 6 of different numbers can be more easily chosen, certainly, the usage quantity of sub-cup 6 is not limited to the number indicated in Fig. 3
Amount.Therefore, a kind of high-throughput Biocontrol microorganism screening plant of the present embodiment, easy to operate, easily controllable inoculation cup puts position
It sets and is not easy to turn upside down, can be with a variety of Biocontrol microorganism ferment filtrates of single treatment, test especially more to processing can obviously add
Fast test process；And sub-cup can be rotated around main cup, select sub-cup more convenient.
Embodiment 2
As the improvement to embodiment 1, difference from example 1 is that, the slot 91 of 9 end of horizon bar is
Rectangular channel, the purpose designed in this way is: can prevent sub-cup 6 from causing sub-cup 6 to overturn around 10 radial rotating of connecting rod.
Embodiment 3
It is improved as the another kind to embodiment 1, difference from example 1 is that, the pin of main 4 bottom of cup
41 are set as two or more, and pin 41 is set as two as shown in figure 1, and the purpose designed in this way is: main cup 4 can be made to put
It sets and is more consolidated in culture dish 1, in addition, can prevent main cup 4 from also and then rotating when rotating sub-cup 6.
The high-throughput Biocontrol microorganism screening plant of the present embodiment in use, in order to improve experiment controllability and accuracy rate,
Culture medium is poured into culture dish 1 referring to the scale in culture dish 1, main cup 4 and sub-cup 6, and the body of culture medium is added in culture dish 1
Product, which is maintained an equal level with culture medium upper surface with main cup 4 and 6 lower openings of sub-cup, to be advisable, it is excessively high will affect antagonistic effect performance (or
Reduce the size of inhibition zone), actual measured value will be relatively low；Too low not to be bonded, ferment filtrate can leak out around from low side, have
Inhibition zone may be increased, actual measured value will be bigger than normal；Then can rotate sub-cup 6 adjust sub-cup 6 position after again to multiple
Strain fermentation filtrate is poured into sub-cup 6 respectively, after then only needing static 2-5min, ware lid 2 is covered, is put into insulating box and cultivates,
Size after 3-5d according to inhibition zone is screened and is evaluated.
The limitation that the technical solution of the utility model is not limited to the above specific embodiments, all skills according to the present utility model
The technology deformation that art scheme is made, each falls within the protection scope of the utility model.
Claims (4)
1. a kind of high throughput Biocontrol microorganism screening plant, including culture dish, the culture dish are equipped with ware lid, which is characterized in that
It further include the inoculation cup being vertically arranged in culture dish, the inoculation cup includes opening and the consistent main cup of height and pair up and down
Cup, the main cup are detachably connected with culture dish bottom, and the main cup outer wall top is equipped with a ring annular groove, assembly in annular groove
There is change, the change outer wall is fixed with multiple horizon bars equally distributed in a ring, is equipped with and inserts in the end of the horizon bar
Slot, the sub-cup are that multiple and sub-cup outer walls are horizontally fixed with connecting rod, and the connecting rod is matched with slot；The culture
The outer wall of ware, main cup and sub-cup is equipped with graduation mark.
2. high throughput Biocontrol microorganism screening plant according to claim 1, it is characterised in that: the culture dish bottom is solid
Surely there is a cushion block, the cushion block upper surface opens up fluted, and the bottom of the main cup is fixed with and the pin for being inserted into groove.
3. high throughput Biocontrol microorganism screening plant according to claim 1 or 2, it is characterised in that: the horizontal rod end
The slot in portion is rectangular channel.
4. high throughput Biocontrol microorganism screening plant according to claim 2, it is characterised in that: the main bottom of a cup portion inserts
Foot is set as two or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820552651.5U CN208218841U (en) | 2018-04-18 | 2018-04-18 | A kind of high throughput Biocontrol microorganism screening plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820552651.5U CN208218841U (en) | 2018-04-18 | 2018-04-18 | A kind of high throughput Biocontrol microorganism screening plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208218841U true CN208218841U (en) | 2018-12-11 |
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ID=64529707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820552651.5U Expired - Fee Related CN208218841U (en) | 2018-04-18 | 2018-04-18 | A kind of high throughput Biocontrol microorganism screening plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208218841U (en) |
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2018
- 2018-04-18 CN CN201820552651.5U patent/CN208218841U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181211 Termination date: 20210418 |