CN103789398A - Soil bacteriostatic activity detection method and application thereof - Google Patents
Soil bacteriostatic activity detection method and application thereof Download PDFInfo
- Publication number
- CN103789398A CN103789398A CN201410088337.2A CN201410088337A CN103789398A CN 103789398 A CN103789398 A CN 103789398A CN 201410088337 A CN201410088337 A CN 201410088337A CN 103789398 A CN103789398 A CN 103789398A
- Authority
- CN
- China
- Prior art keywords
- soil
- measuring method
- rifle head
- liquid
- fungistasis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002689 soil Substances 0.000 title claims abstract description 117
- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title abstract description 6
- 239000000654 additive Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 26
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 9
- 230000000996 additive effect Effects 0.000 claims description 22
- 239000000284 extract Substances 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 17
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- 244000053095 fungal pathogen Species 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 241000228230 Aspergillus parasiticus Species 0.000 claims description 5
- 230000001717 pathogenic effect Effects 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 244000000000 soil microbiome Species 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a soil bacteriostatic activity detection method and the application thereof. The method comprises the steps that a certain amount of soil lixivium is obtained, a certain soil pathogenic bacterium is inoculated, micro-cultivation is carried out in the head of a pipette, by detecting the growth amount of pathogenic bacteria, soil bacteriostatic activity is detected, and the bacteriostatic capacity of the soil can be quantized in a macroscopic mode. Meanwhile, the detection method can be used for detecting the bacteriostatic activity of the soil in which soil additives are used, and accordingly soil additives with high soil bacteriostatic activity can be screened out.
Description
Technical field
The present invention relates to a kind of measuring method of fungistasis of soil activity, and in the application of screening in soil additive.
Background technology
There is widely antibacterial type soil (pressing down sick type soil) in occurring in nature, it is generally acknowledged that pathogenic bacteria can not surely grow in these soil, or can surely grow but endanger very little or be safe from harm.Antibacterial type soil generally can be divided into common antibacterial soil and special antibacterial soil, common antibacterial soil does not generally have transitivity, but special antibacterial soil has transitivity, can cultivate by certain agricultural measures, but but lack suitable method for the macro-level quantitative of fungistasis of soil activity always.
The meta-bolites of Soil Microorganism will act on plant, first these materials will be dissolved in the water of plant rhizosphere soil, therefore the bacteriostatic activity of soil extract can react the bacteriostatic activity of soil itself, so measure the bacteriostatic activity of soil extract, can quantize from macroscopic view the bacteriostasis of soil.Plant soil-borne diseases is mainly fungal disease, so soil extract can be to a certain extent for characterizing the bacteriostatic activity of soil to the inhibition ability of fungal diseases of plants.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, and a kind of measuring method of fungistasis of soil activity is provided
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of measuring method of fungistasis of soil activity, it is characterized in that, get a certain amount of soil extract, inoculate a certain pathogenic soil bacterium, in liquid-transfering gun rifle head, carry out micro-cultivation, measure by the increment to pathogenic bacteria, measure the bacteriostatic activity of soil.
Described measuring method specifically comprises the following steps:
(1) collection of soil extract: will add deionized water in soil to be determined; Hold over night; Collect the liquid of the leaching of soil, centrifugal, get supernatant liquor, carry out the filtration sterilization processing of supernatant liquor with millipore filtration, collect aseptic soil extract, for subsequent use;
(2) inoculation of pathogenic soil bacterium, cultivation: the dry practice head after weighing is carried out to sterilizing, soil extract is added in dry practice head, first wrap up with liquid-leakage preventing with parafilm film rifle head lower end, rifle head is put into glass test tube, add nutrient solution, pathogenic bacteria liquid, aseptic plastic lid on rifle head upper end cover, test tube is placed in bubble chamber and carries out moisturizing cultivation, and culture temperature is 28 ℃, and incubation time is 4 to 6d; Needle-penetration parafilm film for late stage of culture, carries out rifle head centrifugal, removes liquid, and mycelium is still retained in rifle head, removes after the parafilm film of rifle head lower end, and rifle head is weighed;
(3) repeating step (2), replaces with sterilized water by soil extract, as a control group;
(4) calculating of mycelium weight: the weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (2), it is poor to do, and draws cultivation group mycelium weight W2;
The weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (3), it is poor to do, and draws control group mycelium weight W1;
(5) calculating of fungistasis of soil activity:
Iw=（W1-W2）/W1*100%；
Wherein: Iw is fungistasis of soil activity.
Further, in described step (1), 60 parts of soil are divided and installed in three flowerpots, in each flowerpot, add 3 parts of deionized waters respectively, after leaving standstill, by the leaching liquid mixing in three flowerpots, centrifugal, get supernatant liquor, filtration sterilization, collects aseptic soil extract.
Wherein, time of repose is 12 hours, and centrifugal speed is 10000rpm.
Nutrient solution in described step (2) is fungus culture medium, and described pathogenic bacteria liquid is pathogenic fungi spore liquid.Preferably, described fungus culture medium is GY nutrient solution.
In described step (2), the volume ratio of soil extract, nutrient solution, pathogenic fungi spore liquid is 7:1:0.05, and in described step (2), the centrifugal speed of rifle head is 1000rpm, and centrifugation time is 40s.
Preferably, described pathogenic fungi spore liquid is Aspergillus parasiticus spore suspension.
Another object of the present invention is to, provide a kind of measuring method of fungistasis of soil activity in the application of screening in soil additive.
The technical scheme adopting is: utilize the measuring method of above-mentioned fungistasis of soil activity, measure the bacteriostatic activity of the soil of having used soil additive, to screen the active high soil additive of fungistasis of soil.
Further, comprise the steps:
(1) to using the soil of soil additive A, utilize the measuring method of described fungistasis of soil activity, measure the active Iw of its fungistasis of soil
a;
(2) to not using the soil of any soil additive, utilize measuring method as claimed in claim 1, measure the active Iw of its fungistasis of soil
0;
(3) work as Iw
ahigher than Iw
0time, the bacteriostatic activity that soil additive A can specific increase soil is described.
Advantage and positively effect that the present invention has are: measuring method of the present invention, utilize the micro-culture method of Tip-culture, detection by quantitative goes out the bacteriostatic activity of soil extract, realize the bacteriostasis that quantizes soil from macroscopic view, can judge various antibacterial soil, utilize this measuring method simultaneously, can judge accurately the enhancement of soil additive to fungistasis of soil ability.
Embodiment
Embodiment 1
Choose two kinds of soil additives: soil additive A, soil additive B.These two kinds of soil additives are applied to soil, form soil additive A group, soil additive B group, by the soil of not using any soil additive as a control group; Test is carried out under potted plant condition, and three groups of soil carries out bacteriostatic activity detection using after 30 days, 60 days, 90 days respectively, take Aspergillus parasiticus spore liquid as pathogenic fungi inoculation liquid.
1. the detection of fungistasis of soil activity;
Respectively the soil of three of three groups of soil time periods is carried out to the processing of following steps:
The collection of 1.1 soil extracts:
Soil to be determined 6kg is divided and installed in three flowerpots with pallet; In each flowerpot, water 300mL; After static placement 12h, collect the leach liquor in three pallets, mix; Leach liquor is carried out centrifugal, centrifugal speed is: 10000rpm.Get supernatant and the supernatant after centrifugal is carried out to filtration sterilization processing with millipore filtration, collect the aseptic soil extract after filtering, for subsequent use.
The inoculation of 1.2 pathogenic soil bacterium, cultivation:
First the soil extract after 700 μ L being filtered adds in the aseptic 5mL dry practice head that weighs up weight, and wrap up in case rifle head is put into the glass test tube on test-tube stand by leak-stopping liquid with parafilm film in advance rifle head lower end; Add 100 μ L GY nutrient solutions (GY nutrient solution is common glucose, yeast culture liquid, adds 5g/L yeast powder form by 20g/L glucose solution); Add the Aspergillus parasiticus spore suspension of 5 μ L, aseptic plastic cover on rifle head upper end cover; Test-tube stand is placed on to bottom to be covered with in the bubble chamber of wet gauze and to carry out moisturizing cultivation; Culture temperature is 28 ℃, and incubation time is 4 to 6d; Cultivate after date needle-penetration parafilm film, and centrifugal 40s removes the liquid of cultivating in rifle head under 1000rpm condition, now mycelium is retained in rifle head; Then remove the parafilm film of rifle head lower end with tweezers, and rifle head is weighed.
1.3 repeating steps (2), replace with sterilized water by soil extract, as a control group;
The calculating of 1.4 mycelium weights: the weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (2), it is poor to do, and draws cultivation group mycelium weight W2;
The weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (3), it is poor to do, and draws control group mycelium weight W1;
The calculating of 1.5 fungistasis of soil activity:
Iw=（W1-W2）/W1*100%。
2. experimental result
Carry out data processing take the pure culture of Aspergillus parasiticus spore as contrast, test-results is as following table:
3. experiment conclusion
From the result of experiment see use soil additive A group fungistasis of soil activity use 30 days, 60 days with 90 days measured quantity all higher than not using soil additive group and using soil additive B group.The bacteriostatic activity of this explanation specific increase soil of soil additive A energy under condition of pot.
Above preferred embodiment of the present invention is had been described in detail, but described content is only preferred embodiment of the present invention, can not be considered to for limiting practical range of the present invention.All equalization variation and improvement etc. of doing according to the present patent application scope, within all should still belonging to patent covering scope of the present invention.
Claims (10)
1. a measuring method for fungistasis of soil activity, is characterized in that, gets a certain amount of soil extract, inoculates a certain pathogenic soil bacterium, in liquid-transfering gun rifle head, carries out micro-cultivation, measures by the increment to pathogenic bacteria, measures the bacteriostatic activity of soil.
2. measuring method according to claim 1, is characterized in that: described measuring method specifically comprises the following steps:
(1) collection of soil extract: will add deionized water in soil to be determined, hold over night; Collect the liquid of the leaching of soil, centrifugal, get supernatant liquor, carry out the filtration sterilization processing of supernatant liquor with millipore filtration, collect aseptic soil extract, for subsequent use;
(2) inoculation of pathogenic soil bacterium, cultivation: the dry practice head after weighing is carried out to sterilizing, soil extract is added in dry practice head, first wrap up with liquid-leakage preventing with parafilm film rifle head lower end, rifle head is put into glass test tube, add nutrient solution, pathogenic bacteria liquid, aseptic plastic lid on rifle head upper end cover, test tube is placed in bubble chamber and carries out moisturizing cultivation, and culture temperature is 28 ℃, and incubation time is 4 to 6d; Needle-penetration parafilm film for late stage of culture, carries out rifle head centrifugal, removes liquid, and mycelium is still retained in rifle head, removes after the parafilm film of rifle head lower end, and rifle head is weighed;
(3) repeating step (2), replaces with sterilized water by soil extract, as a control group;
(4) calculating of mycelium weight: the weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (2), it is poor to do, and draws cultivation group mycelium weight W2;
The weight of dry practice head and the weight of cultivating rear rifle head before the cultivation weighing according to step (3), it is poor to do, and draws control group mycelium weight W1;
(5) calculating of fungistasis of soil activity:
Iw=（W1-W2）/W1*100%；
Wherein: Iw is fungistasis of soil activity.
3. measuring method according to claim 2, it is characterized in that: in described step (1), 60 parts of soil are divided and installed in three flowerpots, in each flowerpot, add 3 parts of deionized waters respectively, after leaving standstill, by the leaching liquid mixing in three flowerpots, centrifugal, get supernatant liquor, filtration sterilization, collects aseptic soil extract.
4. measuring method according to claim 3, is characterized in that: time of repose is 12 hours, and centrifugal speed is 10000rpm.
5. measuring method according to claim 2, is characterized in that: the nutrient solution in described step (2) is fungus culture medium, and described pathogenic bacteria liquid is pathogenic fungi spore liquid.
6. measuring method according to claim 5, is characterized in that: described fungus culture medium is GY nutrient solution.
7. measuring method according to claim 5, it is characterized in that: in described step (2), the volume ratio of soil extract, nutrient solution, pathogenic fungi spore liquid is 7:1:0.05, in described step (2), the centrifugal speed of rifle head is 1000rpm, and centrifugation time is 40s.
8. measuring method according to claim 7, is characterized in that: described pathogenic fungi spore liquid is Aspergillus parasiticus spore suspension.
9. an application for measuring method as claimed in claim 1, is characterized in that: utilize described measuring method, measure the bacteriostatic activity of the soil of having used soil additive, to screen the active high soil additive of fungistasis of soil.
10. application according to claim 9, is characterized in that, comprises the steps:
(1) to using the soil of soil additive A, utilize measuring method as claimed in claim 1, measure the active Iw of its fungistasis of soil
a;
(2) to not using the soil of any soil additive, utilize measuring method as claimed in claim 1, measure the active Iw of its fungistasis of soil
0;
(3) work as Iw
ahigher than Iw
0time, the bacteriostatic activity that soil additive A can specific increase soil is described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410088337.2A CN103789398A (en) | 2014-03-11 | 2014-03-11 | Soil bacteriostatic activity detection method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410088337.2A CN103789398A (en) | 2014-03-11 | 2014-03-11 | Soil bacteriostatic activity detection method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103789398A true CN103789398A (en) | 2014-05-14 |
Family
ID=50665452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410088337.2A Pending CN103789398A (en) | 2014-03-11 | 2014-03-11 | Soil bacteriostatic activity detection method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103789398A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106556639A (en) * | 2016-12-02 | 2017-04-05 | 临沂大学 | A kind of liquid of soil fungi phosphorus component31P NMR assay methods |
| CN108265100A (en) * | 2018-03-26 | 2018-07-10 | 成都知联汇科技有限公司 | A kind of assay method of bacteriostatic activity of biologic extract |
| CN108776222A (en) * | 2018-06-14 | 2018-11-09 | 吉林省农业科学院 | Gongzhuling mycin bacteriostatic activity detection method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070218522A1 (en) * | 2005-03-17 | 2007-09-20 | Phigenics Llc | Methods and compositions for rapidly detecting and quantifying viable legionella |
| CN101413876A (en) * | 2008-11-21 | 2009-04-22 | 天津科技大学 | Micropore plate biological detection method for rapidly detecting bacteriostatics activity |
-
2014
- 2014-03-11 CN CN201410088337.2A patent/CN103789398A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070218522A1 (en) * | 2005-03-17 | 2007-09-20 | Phigenics Llc | Methods and compositions for rapidly detecting and quantifying viable legionella |
| CN101413876A (en) * | 2008-11-21 | 2009-04-22 | 天津科技大学 | Micropore plate biological detection method for rapidly detecting bacteriostatics activity |
Non-Patent Citations (1)
| Title |
|---|
| 王琢: "黄曲霉毒素生防细菌的分子鉴定及其抑毒活性物质研究", 《中国优秀硕士学位论文全文数据库基础学辑》, no. 2, 15 February 2009 (2009-02-15) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106556639A (en) * | 2016-12-02 | 2017-04-05 | 临沂大学 | A kind of liquid of soil fungi phosphorus component31P NMR assay methods |
| CN108265100A (en) * | 2018-03-26 | 2018-07-10 | 成都知联汇科技有限公司 | A kind of assay method of bacteriostatic activity of biologic extract |
| CN108776222A (en) * | 2018-06-14 | 2018-11-09 | 吉林省农业科学院 | Gongzhuling mycin bacteriostatic activity detection method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104651239B (en) | A kind of promotion dendrobium devonianum seeds sprout the bacterial strain to form seedling and its application | |
| CN103468587A (en) | Fungi strain of Tulasnella and application thereof in promotion of germination of Cymbidium mannii seeds | |
| CN106318875B (en) | Bidirectional artificial culture method of cordyceps sobolifera | |
| CN104805019B (en) | One plant of endogenetic fungus that can promote aleurite montana Nutrient Absorption | |
| CN103436448A (en) | Method for preserving strain of ustilaginoidea virens for long time | |
| CN107881132A (en) | A kind of the fixed nitrogen phosphorus decomposing Promoting bacteria that can be used for desert plant and its microbial inoculum are applied with it | |
| CN103436465B (en) | Alcaligenes faecalis strain | |
| CN103468588A (en) | Tulasnella strain and application thereof in promotion of germination of Dendrobium aphyllum seeds | |
| CN101381685A (en) | Preparation method of tobacco black shank inoculum | |
| CN104756754B (en) | A kind of cultural method of Antrodia camphorata fructification | |
| CN102787084A (en) | Bacillus methylotrophicus 4-L-16 for prevention and control of banana vascular wilt and its application | |
| CN103789398A (en) | Soil bacteriostatic activity detection method and application thereof | |
| CN101558766A (en) | Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof | |
| CN105420172B (en) | Microbial inoculum and the application of a kind of metribuzin pesticide residual degrading bacteria and its production | |
| CN102586143B (en) | Bacillus mojavensis and microorganism bacterium agent thereof for controlling cucumber downy mildew | |
| CN106613276A (en) | Artemisia apiacea seedling cultivating method and special trichoderma atroviride fertilizer thereof | |
| CN104403952B (en) | A kind of sclerotium oyster mushroom novel bacterial and its cultural method and application | |
| CN102696755A (en) | Method for improving efficacy of cryptococcus laurentii with control on penicilliosis and patulin of post-picked apples | |
| CN108103001A (en) | A kind of method that phytophthora infestans is promoted to generate a large amount of sporangiums | |
| CN105602857B (en) | A kind of optimization method that wild cicada fungus liquid spawn is manually cultivated | |
| Lian et al. | In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato | |
| CN106190898A (en) | A kind of industrialization liquid fermentation method of the bacillus cereus JZBC1 dissolving pond dinoflagellate | |
| CN106167777A (en) | A kind of cultural method of the bacillus amyloliquefaciens producing Substance | |
| CN203613177U (en) | Culture device applicable to microorganism separation and purification | |
| CN110373331A (en) | The Huperzia serrata endogenetic epiphyte of one plant of anti-botrytis cinerea and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: HARBIN INSTITUTE OF TECHNOLOGY (WEIHAI) Free format text: FORMER OWNER: XIAO WEI Effective date: 20140821 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yan Peisheng Inventor after: Faiz Mohamed Inventor after: Mohamed Aslam Buzda Inventor after: Xiao Wei Inventor after: Gong Na Inventor after: Chen Xun Inventor after: Wang Na Inventor after: OUYANG XINLAN Inventor after: Fei Xiaodan Inventor after: Wu Yan Inventor after: Liu Guoli Inventor before: Xiao Wei Inventor before: Mohamed Aslam Buzda Inventor before: Gong Na Inventor before: Chen Xun Inventor before: Wang Na Inventor before: OUYANG XINLAN Inventor before: Fei Xiaodan Inventor before: Wu Yan Inventor before: Liu Guoli Inventor before: Faiz Mohamed |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: XIAO WEI GONG NA CHEN XUN WANG NA OUYANG XINLAN FEI XIAODAN WU YAN LIU GUOLI FAIZ MOHAMED MOHAMED ASLAM BUZDAR TO: YAN PEISHENG XIAO WEI GONG NA CHEN XUN WANG NA OUYANG XINLAN FEI XIAODAN WU YAN LIU GUOLI FAIZ MOHAMED MOHAMED ASLAM BUZDAR |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20140821 Address after: 264209 Weihai Cultural Road West, Shandong, No. 2 Applicant after: Harbin Institute of Technology (Weihai) Address before: 264209 Harbin Institute of Technology, 2 West Wenhua Road, Shandong, Weihai Applicant before: Xiao Wei |
|
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140514 |