CN206502831U - A kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks - Google Patents
A kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks Download PDFInfo
- Publication number
- CN206502831U CN206502831U CN201720044020.8U CN201720044020U CN206502831U CN 206502831 U CN206502831 U CN 206502831U CN 201720044020 U CN201720044020 U CN 201720044020U CN 206502831 U CN206502831 U CN 206502831U
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- bottle
- culture
- orifice plate
- tissue block
- plate suspension
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Abstract
The utility model is related to a kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks, it is a horizontal offset flat shape bottle provided with blake bottle described in culture bottle, the offset flat shape bottle, which internally fills to be horizontally disposed with, an orifice plate suspension, the orifice plate suspension is linked into an integrated entity structure by two sides and two bottles of culture bottle, several circular grooves are evenly arranged with the orifice plate suspension, a diameter of 4mm of the circular groove, the depth of the circular groove is 4mm, it is 1mm that orifice plate, which is suspended in bottom surface with blake bottle floor height, the upper surface of orifice plate suspension is smooth, the culture bottle is provided with bottleneck in side, the bottleneck is provided with bottle cap.
Description
Technical field
The utility model belongs to a kind of experimental ware, particularly a kind of blake bottle for cultivating umbilical cord tissue cell.
Background technology
Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to be present in neonatal umbilical cord tissue
In a kind of versatile stem cell, it can be divided into many kinds of histocytes, with wide potential applicability in clinical practice.
Fresh and healthy umbilical cord is taken, after being rinsed well with PBS, blood vessel is removed with scissors tweezers, the Fahrenheit glue group of the inside is stripped out
Knit, gained tissue is fully shredded to 1mm3 sizes, add α-MEM nutrient solutions and be placed in 37 DEG C, 5% CO2 incubator cultures, training
Contain 10%FBS, 100U/ml penicillin, 100U/ml streptomysins in nutrient solution.After umbilical cord tissue culture 5-7 days, it is seen that have part thin
Born of the same parents climb out of around tissue block, and form is in tiny fusiformis, after one week, and cell starts rapid propagation, and it is thin that formation is differed in size
Born of the same parents' colony, after cell is covered with, is passed on 0.25% Trypsin Induced.After umbilical cord tissue block is adherent, culture a period of time, slowly
Snail-like movement goes out cell, adherent very crucial.
Current technological deficiency：Tissue block is added culture medium to add blake bottle together by prior art, and natural subsidence is adherent, and one is
There is certain randomness, adherent tissue block is few, two be to change liquid, or during observation, mobile blake bottle, tissue block is easy to come off
Fall.
Utility model content
The purpose of this utility model is a kind of umbilical cord tissue block adherent method culture fixation group of proposition in order to solve the above problems
The culture bottle of block is knitted, tissue block will be fixed on by the culture bottle of the umbilical cord tissue block adherent method culture fixing organization blocks
In groove, blake bottle is translated, tissue block will not fall off, and add the chance that tissue block grows cell, while process is simplified, subtracted
Few incubation time, it is easy to operate.
The purpose of this utility model is achieved through the following technical solutions：Umbilical cord tissue block adherent method culture fixation group
Knit the culture bottle of block, it is characterised in that：It is a horizontal offset flat shape bottle provided with blake bottle described in culture bottle, it is described inclined
Flat-shaped bottle, which internally fills to be horizontally disposed with, an orifice plate suspension, and the orifice plate suspension passes through two sides and two bottles of culture bottle
Link into an integrated entity structure, is evenly arranged with several circular grooves on the orifice plate suspension, the circular groove it is a diameter of
4mm, the depth of the circular groove is 4mm, and it is 1mm that orifice plate, which is suspended in bottom surface with blake bottle floor height, orifice plate suspension
Upper surface is smooth, and the culture bottle is provided with bottleneck in side, and the bottleneck is provided with bottle cap.
The utility model simplifies process, reduces incubation time, easy to operate；Tissue block is fixed in groove, put down
Blake bottle is moved, tissue block will not fall off, and considerably increase the chance that tissue block grows cell.
Brief description of the drawings
Fig. 1 is overall structure diagram of the present utility model；
Fig. 2 is orifice plate suspension frame structure schematic diagram of the present utility model；
Embodiment
The utility model is further described with reference to the accompanying drawings and examples：
Embodiment：Referring to accompanying drawing 1,2, the culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks, provided with training
Bottle 1 is supported, the blake bottle is a horizontal offset flat shape bottle, and the offset flat shape bottle, which internally fills to be horizontally disposed with, an orifice plate
Suspension 2, the orifice plate suspension is linked into an integrated entity structure by two sides and two bottles of culture bottle, on the orifice plate suspension
It is even to be provided with several circular grooves 3, a diameter of 4mm of the circular groove, the depth of the circular groove is 4mm, orifice plate
It is 1mm that bottom surface, which is suspended in, with blake bottle floor height, and the upper surface of orifice plate suspension is smooth, and the culture bottle is in side
Provided with bottleneck 4, the bottleneck is provided with bottle cap 5.
On blake bottle I draw black line region be orifice plate region, orifice plate be similar to 96 orifice plates, aperture 4mm, height 4mm, away from
From blake bottle bottom surface 1mm, orifice plate upper surface is smooth, and orifice plate both sides and blake bottle side wall are integral, are associated on the wall of side.
This is a special blake bottle.During culture, tissue block is cut into 2-4mm blocks grain first, then with the pipette for the tip that fractures
Draw block grain to add on blake bottle orifice plate, then spread tissue block in hole with pipette or cell scraper.Nutrient solution is added again
To the blake bottle bottom surface near from bottleneck.Blake bottle lies in culture in CO2gas incubator.Because orifice plate is apart from blake bottle bottom
There is a 1mm distance in face, and cell grows rear adherent in blake bottle bottom surface from tissue block, and tissue block is in hole, without floating, hole
Plate and blake bottle one, orifice plate will not be moved.A certain distance, and orifice plate height are arranged at orifice plate region distance blake bottle bottom
It is very big apart from blake bottle upper table space of planes, it is easy to pipette is reached into bottom and draws liquid, culture is not influenceed.
Finally it should be noted that：Obviously, above-described embodiment is only intended to clearly illustrate the utility model example,
And the not restriction to embodiment.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.And thus
Among the obvious changes or variations extended out is still in protection domain of the present utility model.
Claims (1)
1. a kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks, it is characterised in that：Provided with culture bottle
(1), the blake bottle is a horizontal offset flat shape bottle, and the offset flat shape bottle, which internally fills to be horizontally disposed with, an orifice plate suspension
(2), the orifice plate suspension is linked into an integrated entity structure by two sides and two bottles of culture bottle, uniform on the orifice plate suspension
It is provided with several circular grooves (3), a diameter of 4mm of the circular groove, the depth of the circular groove is 4mm, orifice plate
It is 1mm that bottom surface, which is suspended in, with blake bottle floor height, and the upper surface of orifice plate suspension is smooth, and the culture bottle is in side
Provided with bottleneck (4), the bottleneck is provided with bottle cap (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720044020.8U CN206502831U (en) | 2017-01-16 | 2017-01-16 | A kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720044020.8U CN206502831U (en) | 2017-01-16 | 2017-01-16 | A kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206502831U true CN206502831U (en) | 2017-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720044020.8U Expired - Fee Related CN206502831U (en) | 2017-01-16 | 2017-01-16 | A kind of culture bottle of umbilical cord tissue block adherent method culture fixing organization blocks |
Country Status (1)
| Country | Link |
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| CN (1) | CN206502831U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112266875A (en) * | 2020-09-25 | 2021-01-26 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
-
2017
- 2017-01-16 CN CN201720044020.8U patent/CN206502831U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112266875A (en) * | 2020-09-25 | 2021-01-26 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
| CN112266875B (en) * | 2020-09-25 | 2021-10-29 | 生物岛实验室 | Cell culture container and method for culturing primary cells |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170919 Termination date: 20200116 |
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| CF01 | Termination of patent right due to non-payment of annual fee |